CN116355898A - miRNA-133在调节绵羊胚胎毛囊发育中的应用 - Google Patents
miRNA-133在调节绵羊胚胎毛囊发育中的应用 Download PDFInfo
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- CN116355898A CN116355898A CN202211217743.5A CN202211217743A CN116355898A CN 116355898 A CN116355898 A CN 116355898A CN 202211217743 A CN202211217743 A CN 202211217743A CN 116355898 A CN116355898 A CN 116355898A
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Abstract
Description
miR-133 mimic | mimic-NC | miR-133 inhibitor | inhibitor-NC | |
24h | 0.141 | 0.161 | 0.157 | 0.161 |
48h | 0.168 | 0.172 | 0.171 | 0.159 |
72h | 0.219 | 0.246 | 0.226 | 0.231 |
96h | 0.357 | 0.421 | 0.383 | 0.349 |
5%浓缩胶 | For 1gel | For 2gel |
ddH2O | 1.4mL | 2.7mL |
0.5M Tris pH6.8 | 250μL | 500μL |
30% Acrylamide | 330μL | 670μL |
10% SDS | 20μL | 40μL |
10% APS | 20μL | 40μL |
TMEM | 2μL | 4μL |
10%分离胶 | For 1gel | For 2gel |
ddH2O | 1.9 mL | 4.0 mL |
1.5M Tris pH8.8 | 1.3 mL | 2.5 mL |
30% Acrylamide | 1.7 mL | 3.3 mL |
10% SDS | 50μL | 100μL |
10% APS | 50μL | 100μL |
TMEM | 2μL | 4μL |
Claims (10)
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CN202210747140.XA CN114891796A (zh) | 2022-06-28 | 2022-06-28 | miRNA-133在调节绵羊胚胎毛囊发育中的应用 |
CN202210747140X | 2022-06-28 |
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CN116355898A true CN116355898A (zh) | 2023-06-30 |
CN116355898B CN116355898B (zh) | 2024-05-14 |
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CN202211217743.5A Active CN116355898B (zh) | 2022-06-28 | 2022-09-30 | miRNA-133在调节绵羊胚胎毛囊发育中的应用 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116970610A (zh) * | 2023-09-21 | 2023-10-31 | 浙江大学海南研究院 | 一种chi-miR-335-5p的新用途及其调控靶基因DKK1表达的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040247573A1 (en) * | 2001-12-18 | 2004-12-09 | Jung-Chul Kim | Dermal replacement prepared from mesenchymal cells of hair follicle |
US20060185027A1 (en) * | 2004-12-23 | 2006-08-17 | David Bartel | Systems and methods for identifying miRNA targets and for altering miRNA and target expression |
JP2014223047A (ja) * | 2013-05-14 | 2014-12-04 | 杏林製薬株式会社 | アポトーシス抵抗性特発性肺線維症モデル |
CN111154878A (zh) * | 2020-02-14 | 2020-05-15 | 北京粒基生物科技有限公司 | 一组miRNA在制备作为用于早期乳腺癌筛查的标志物中的应用 |
CN113186306A (zh) * | 2021-06-11 | 2021-07-30 | 内蒙古农业大学 | 一种与羊的皮肤毛囊成熟相关的miRNA及应用 |
CN113913532A (zh) * | 2021-10-25 | 2022-01-11 | 扬州大学 | 一种与湖羊毛乳头细胞增殖相关的miRNA标志物及其检测引物和应用 |
-
2022
- 2022-06-28 CN CN202210747140.XA patent/CN114891796A/zh active Pending
- 2022-09-30 CN CN202211217743.5A patent/CN116355898B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040247573A1 (en) * | 2001-12-18 | 2004-12-09 | Jung-Chul Kim | Dermal replacement prepared from mesenchymal cells of hair follicle |
US20060185027A1 (en) * | 2004-12-23 | 2006-08-17 | David Bartel | Systems and methods for identifying miRNA targets and for altering miRNA and target expression |
JP2014223047A (ja) * | 2013-05-14 | 2014-12-04 | 杏林製薬株式会社 | アポトーシス抵抗性特発性肺線維症モデル |
CN111154878A (zh) * | 2020-02-14 | 2020-05-15 | 北京粒基生物科技有限公司 | 一组miRNA在制备作为用于早期乳腺癌筛查的标志物中的应用 |
CN113186306A (zh) * | 2021-06-11 | 2021-07-30 | 内蒙古农业大学 | 一种与羊的皮肤毛囊成熟相关的miRNA及应用 |
CN113913532A (zh) * | 2021-10-25 | 2022-01-11 | 扬州大学 | 一种与湖羊毛乳头细胞增殖相关的miRNA标志物及其检测引物和应用 |
Non-Patent Citations (6)
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116970610A (zh) * | 2023-09-21 | 2023-10-31 | 浙江大学海南研究院 | 一种chi-miR-335-5p的新用途及其调控靶基因DKK1表达的方法 |
CN116970610B (zh) * | 2023-09-21 | 2023-12-29 | 浙江大学海南研究院 | 一种chi-miR-335-5p的新用途及其调控靶基因DKK1表达的方法 |
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CN114891796A (zh) | 2022-08-12 |
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