CN116326585A - Nematicide composition for improving prevention and control effect of taro root knot nematode disease - Google Patents
Nematicide composition for improving prevention and control effect of taro root knot nematode disease Download PDFInfo
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- CN116326585A CN116326585A CN202310249496.5A CN202310249496A CN116326585A CN 116326585 A CN116326585 A CN 116326585A CN 202310249496 A CN202310249496 A CN 202310249496A CN 116326585 A CN116326585 A CN 116326585A
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- knot nematode
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- lentinan
- taro
- sulfoxaflor
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- 241000243785 Meloidogyne javanica Species 0.000 title claims abstract description 36
- 239000000203 mixture Substances 0.000 title claims abstract description 24
- 244000205754 Colocasia esculenta Species 0.000 title claims abstract description 20
- 235000006481 Colocasia esculenta Nutrition 0.000 title claims abstract description 20
- 201000010099 disease Diseases 0.000 title claims abstract description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 13
- 230000000694 effects Effects 0.000 title claims abstract description 13
- 239000005645 nematicide Substances 0.000 title claims abstract description 13
- 230000002265 prevention Effects 0.000 title claims abstract description 11
- 229920001491 Lentinan Polymers 0.000 claims abstract description 29
- 229940115286 lentinan Drugs 0.000 claims abstract description 29
- 239000005934 Sulfoxaflor Substances 0.000 claims abstract description 17
- ZVQOOHYFBIDMTQ-UHFFFAOYSA-N [methyl(oxido){1-[6-(trifluoromethyl)pyridin-3-yl]ethyl}-lambda(6)-sulfanylidene]cyanamide Chemical compound N#CN=S(C)(=O)C(C)C1=CC=C(C(F)(F)F)N=C1 ZVQOOHYFBIDMTQ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000011282 treatment Methods 0.000 claims abstract description 17
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- 230000001069 nematicidal effect Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 abstract description 14
- 230000002195 synergetic effect Effects 0.000 abstract description 9
- 150000001875 compounds Chemical class 0.000 abstract description 5
- 239000000575 pesticide Substances 0.000 abstract description 4
- 238000012827 research and development Methods 0.000 abstract description 4
- AWSZRJQNBMEZOI-UHFFFAOYSA-N 2-methoxyethyl 2-(4-tert-butylphenyl)-2-cyano-3-oxo-3-[2-(trifluoromethyl)phenyl]propanoate Chemical compound C=1C=C(C(C)(C)C)C=CC=1C(C#N)(C(=O)OCCOC)C(=O)C1=CC=CC=C1C(F)(F)F AWSZRJQNBMEZOI-UHFFFAOYSA-N 0.000 description 11
- 239000005655 Cyflumetofen Substances 0.000 description 11
- 241000244206 Nematoda Species 0.000 description 11
- DNVLJEWNNDHELH-UHFFFAOYSA-N thiocyclam Chemical compound CN(C)C1CSSSC1 DNVLJEWNNDHELH-UHFFFAOYSA-N 0.000 description 10
- 241000243786 Meloidogyne incognita Species 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 231100000419 toxicity Toxicity 0.000 description 7
- 230000001988 toxicity Effects 0.000 description 7
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- 239000003814 drug Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 2
- 240000000599 Lentinula edodes Species 0.000 description 2
- 235000001715 Lentinula edodes Nutrition 0.000 description 2
- 241001143352 Meloidogyne Species 0.000 description 2
- 241000243784 Meloidogyne arenaria Species 0.000 description 2
- 241000243787 Meloidogyne hapla Species 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- ZXQYGBMAQZUVMI-UNOMPAQXSA-N cyhalothrin Chemical compound CC1(C)C(\C=C(/Cl)C(F)(F)F)C1C(=O)OC(C#N)C1=CC=CC(OC=2C=CC=CC=2)=C1 ZXQYGBMAQZUVMI-UNOMPAQXSA-N 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
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- 206010052428 Wound Diseases 0.000 description 1
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- 238000012258 culturing Methods 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
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- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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- 150000004676 glycans Chemical class 0.000 description 1
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- 230000000091 immunopotentiator Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
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- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of pesticides, in particular to a nematicide composition for improving the prevention and treatment effect of taro root knot nematode disease. The nematicide composition for improving the prevention and treatment effect of the taro root knot nematode disease comprises the following effective components of lentinan and sulfoxaflor; wherein the mass ratio of the lentinan to the sulfoxaflor is 1-100:100-1. The lentinan and the sulfoxaflor in the nematicide composition have synergistic effect on controlling the root-knot nematode of the south, and compared with a single active ingredient, the nematicide composition can effectively improve the control effect on the root-knot nematode of the taro and can provide support for research and development of a compound agent for controlling the root-knot nematode of the taro.
Description
Technical Field
The invention relates to the technical field of pesticides, in particular to a nematicide composition for improving the prevention and treatment effect of taro root knot nematode disease.
Background
Plant nematode disease is an important disease species in world agricultural production, with losses caused by root-knot nematodes accounting for over 50%. The root knot nematode hosts are wide, can infect more than 3000 plants, including most crops, can generally cause the crop to reduce the yield by 10-20%, and more than 75% in serious cases. More than 90% of plant root knot nematode disease is reported to be caused by 4 nematodes, such as southern root knot nematodes (Meloidogyne incognita), northern root knot nematodes (Meloidogyne hapla), peanut root knot nematodes (Meloidogyne arenaria) and java root knot nematodes (meloidogyne javanica). Root-knot nematodes mainly exist in soil with the depth of 5-30cm, destroy plant root system structures by invading wounds and forming tumor-shaped root knots, absorb nutrient substances, influence normal metabolic activities of plants, cause the coincidence of infection of other pathogenic bacteria, and weaken or even die plant growth vigor, so that crops are reduced in yield and even are in failure.
The taro is also affected by root-knot nematodes in the taro planting process, and the yield and quality of the taros are affected. The chemical agent is a main means for preventing and controlling the root-knot nematode disease in the taro field at present, and has the advantages of quick acting, stability, low cost and the like. Because the drug resistance of the root-knot nematode is accumulated, the drug effect of a single chemical agent is effective under the conventional dosage, and therefore, the research and development of a novel compound agent for preventing and treating the root-knot nematode is necessary.
Lentinan (Lentinan) is an effective active ingredient extracted from fruiting body of Lentinus Edodes of good quality, and is an immunopotentiator. Clinical and pharmacological studies show that lentinan has antiviral, antitumor, immunity regulating and interferon formation stimulating effects. Application number CN201510172220.7 discloses a nematicidal disease-resistant composition and application thereof, and specifically discloses that fluoroolefin sulfone and lentinan are compounded in an amount of 80:1-1:80 has synergistic effect on the co-toxicity coefficient CTC of greater than 120 in the ratio range of cucumber root-knot nematode combined toxicity measurement.
The inventor finds that the lentinan and cyflumetofen, sulfoxaflor or thiocyclam have synergistic effect on preventing and controlling root-knot nematodes when being compounded through indoor experiments.
At present, no report on the compounding of lentinan and cyflumetofen, sulfoxaflor or thiocyclam is found.
Disclosure of Invention
The invention aims to provide the nematicide composition for improving the prevention and treatment effect of the taro root-knot nematode, which has synergistic effect on the prevention and treatment of the root-knot nematode when two active ingredients are compounded, can improve the prevention and treatment effect on the root-knot nematode, effectively prevent and treat the taro root-knot nematode, and can provide support for research and development of a compound agent for preventing and treating the root-knot nematode.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the nematicide composition for improving the prevention and treatment effect of the taro root knot nematode disease comprises the following active ingredients of lentinan and cyflumetofen or sulfoxaflor; wherein the mass ratio of the lentinan to the cyflumetofen, the sulfoxaflor or the thiocyclam is 1-100:100-1.
Preferably, the mass ratio of the lentinan to the cyflumetofen is 1-60:27-1.
Preferably, the mass ratio of the lentinan to the sulfoxaflor is 1:30-5.
Preferably, the mass ratio of the lentinan to the thiocyclam is 1-7:4-1.
Compared with the prior art, the invention has the following beneficial effects:
after being compounded with cyflumetofen, sulfoxaflor or thiocyclam, the lentinan and the nematicide composition of the invention have synergistic effect on controlling the root-knot nematode of south, and compared with single active ingredients, the nematicide composition of the invention can effectively improve the control effect on the root-knot nematode of taros and can provide support for research and development of a compound agent for controlling the root-knot nematode of taros.
Detailed Description
The invention will be better understood from the following examples, which are described only for illustration of the invention and should not be construed as limiting the invention as detailed in the claims.
Examples: indoor bioactivity test of lentinan compound
1. Test object: nannon root-knot nematode (Meloidogyne incognita)
2. Test agent
96% Lentinus Edodes polysaccharide powder (Shanghai Yuan Ye Biotechnology Co., ltd.)
95% cyflumetofen (Shanghai Yuan Ye Biotechnology Co., ltd.)
99% sulfoxaflor (Jiangsu Yiheng pharmaceutical Co., ltd.)
90% thiocyclam (Heilongjiang Ji Xiangnong chemical Co., ltd.)
The raw materials are dissolved by dimethyl sulfoxide to prepare single-dose mother liquor, then the mother liquor is diluted by 0.1% Tween-80 aqueous solution, a plurality of groups of proportions are arranged, 7 gradient mass concentrations of each single dose and each group of proportion mixture are respectively arranged according to an equal ratio method, and each mass concentration liquid medicine is 50mL for standby.
3. The test method comprises the following steps: (reference to NT/Y1833.1-2009 methods for testing nematode dipping in the pesticide section 1 method for inhibiting plant pathogenic nematodes by means of biological assay in pesticide chambers)
And (3) picking eggs of the root knot nematodes from the roots of the taros, cleaning with clear water, placing the eggs on wet filter paper of a culture dish, and incubating at 25 ℃ to obtain second-instar larvae with consistent instar. Eluting the cultured root-knot nematode with water, filtering, centrifuging at 1000r/min for 2min, discarding supernatant, adding water, centrifuging again, and finally re-suspending the root-knot nematode with water to 400 head/mL for use. Sequentially sucking 3mL of liquid medicine from low concentration to high concentration by a pipette, respectively adding the liquid medicine into a test tube, and then sucking the prepared equivalent nematode suspension into the test tube to uniformly mix the liquid medicine and the nematode suspension in equivalent. And (3) transferring a certain volume of the mixed solution into the small holes of the 24-hole biochemical test plate by using a pipette, covering, and culturing for 24 hours at the constant temperature of 25 ℃. Each treatment was repeated 4 times with treatments containing dimethyl sulfoxide and 0.1% tween-80 in water as controls. Taking 1mL of mixed solution from each treatment, observing the death condition of nematodes under an dissecting lens, and recording the number of bus worms and the number of dead nematodes for investigation when the number of nematodes observed per repetition is not less than 100. Wherein the criterion for nematode death is: nematodes are stiff and cannot flex by touching with hairpins or bamboo pins. From the survey data, mortality and corrected mortality for each treatment were calculated.
In the above formula: p- -mortality in units of; k- -number of dead insects; n- -total number of insects treated.
In the above formula: p (P) 1 -correct mortality in units of; p (P) t -mortality rate in units of treatment; p (P) 0 Control mortality in%.
4. Data analysis: regression analysis of the log concentration values of each treatment agent and the corrected mortality probability values of each treatment was performed using DPS software to calculate LC of each treatment agent 50 And the co-toxicity coefficient (CTC value) of the mixture was calculated according to the grand cloud Pei method.
In the above formula: ati—the measured virulence index of the mixture; LC of S-standard agent 50 The unit is mg/L; m- -LC of test agent 50 The unit is mg/L.
TTI＝TI A ×P A +TI B ×P B
In the above formula: TTI- -theoretical toxicological index of the mixture; TI (TI) A -a toxicity index of the agent; p (P) A - -A the percentage of agent in the blend in percent (%); TI (TI) B -a toxicity index of the agent; p (P) B The percentage content of the agent in the mixture is shown as percentage (%).
In the above formula: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—the theoretical toxicity index of the mixture.
5. Evaluation of drug efficacy
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-3.
TABLE 1 toxicity determination results of lentinan and cyflumetofen in combination on meloidogyne incognita
As can be seen from Table 1, at 1-60: in the mass ratio range of 27-1, the co-toxicity coefficient of the lentinan and the cyflumetofen to the meloidogyne incognita is more than 120 after the lentinan and the cyflumetofen are compounded, and the synergistic effect is shown.
TABLE 2 toxicity measurement results of lentinan and sulfoxaflor compounded on meloidogyne incognita
As can be seen from table 2, at 1: within the mass ratio range of 30-5, the co-toxicity coefficient of the lentinan and the sulfoxaflor to the meloidogyne incoordinates is more than 120 after the lentinan and the sulfoxaflor are compounded, and the synergistic effect is shown.
TABLE 3 toxicity determination results of lentinan and thiocyclam combination on meloidogyne incognita
As can be seen from table 3, at 1-7:4-1, and the co-toxicity coefficient of the lentinan and the thiocyclam to the meloidogyne incoordinates is more than 120 after the lentinan and the thiocyclam are compounded, thus the synergistic effect is shown.
In conclusion, after the lentinan is compounded with the cyflumetofen, the sulfoxaflor or the thiocyclam, the biological activities of the cyhalothrin and the cyhalothrin are synergistic, and compared with a single effective component, the control effect on the taro root knot nematode can be effectively improved.
The foregoing is merely illustrative of the preferred embodiments of this invention and modifications, obvious to those skilled in the art, may be made without departing from the principles of this invention and are within the scope of this invention.
Claims (2)
1. The nematicide composition for improving the prevention and treatment effect of the taro root knot nematode disease is characterized in that the active ingredients of the nematicide composition are compounded by lentinan and sulfoxaflor; wherein the mass ratio of the lentinan to the sulfoxaflor is 1-100:100-1.
2. The nematicidal composition for improving prevention and treatment effects of taro root knot nematode disease according to claim 1, wherein the mass ratio of lentinan to sulfoxaflor is 1:30-5.
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| CN202310249496.5A CN116326585B (en) | 2022-06-20 | 2022-06-20 | Nematicide composition for improving prevention and control effect of taro root knot nematode disease |
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| CN202310249496.5A CN116326585B (en) | 2022-06-20 | 2022-06-20 | Nematicide composition for improving prevention and control effect of taro root knot nematode disease |
| CN202210695406.0A CN114885948B (en) | 2022-06-20 | 2022-06-20 | Nematicidal composition for preventing and treating taro root knot nematode disease |
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| CN106973937B (en) * | 2017-05-12 | 2019-12-31 | 广东真格生物科技有限公司 | Nematicidal composition containing cyflumetofen |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN114885948A (en) | 2022-08-12 |
| CN116138257B (en) | 2024-05-03 |
| CN116326585B (en) | 2024-05-07 |
| CN116138257A (en) | 2023-05-23 |
| CN114885948B (en) | 2023-04-07 |
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