CN114041462B - Application of propionamide in preparation of nematocide - Google Patents
Application of propionamide in preparation of nematocide Download PDFInfo
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- CN114041462B CN114041462B CN202111317617.2A CN202111317617A CN114041462B CN 114041462 B CN114041462 B CN 114041462B CN 202111317617 A CN202111317617 A CN 202111317617A CN 114041462 B CN114041462 B CN 114041462B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
- A01N37/04—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof polybasic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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Abstract
The invention relates to the technical field of agricultural microbiology, in particular to application of propionamide in preparing nematicidal preparation, which finds that propionamide has better insecticidal activity to nematodes through determination of lethality of meloidogyne incognita, meloidogyne javanica and soybean cyst nematodes, and LC of propionamide to meloidogyne incognita, meloidogyne javanica and soybean cyst nematodes 50 The values are 22.87 mu g/mL, 107.02 mu g/mL and 46.09 mu g/mL respectively, after the complex preparation is compounded with betaine, the compound preparation is used for the LC of meloidogyne incognita 50 The minimum is 13.92 mu g/mL, and after the compound preparation is compounded with methyl malonic acid, the compound preparation has LC for meloidogyne incognita 50 The lowest value was 14.48. Mu.g/mL. The preparation provides a new resource for the research and development of a novel plant nematode killing preparation.
Description
Technical Field
The invention belongs to the technical field of agricultural microbiology, and particularly relates to an application of propionamide in preparing a nematicidal preparation.
Background
More than 200 species of 5000 nematodes have been found to be able to parasitize plants. Of these, the root-knot nematodes (melodogyne spp.) and cyst nematodes (Heterodera spp. And Globodera spp.).
Root-knot nematodes can parasitize almost all vascular bundles, of which southern root-knot nematodes (m.incognita), northern root-knot nematodes (m.hapla), javanica (m.javanica) and arachis hypogaea (m.arenaria) are the most harmful, and in recent years, the harm of elephantopus japonicus root-knot nematodes (m.enterolobii) is also increasing day by day. Female root-knot nematodes lay eggs in egg masses consisting of protective colloid matrixes, first instar larvae formed by embryonic development can molt in the eggs to become second instar larvae with infection capacity, the second instar larvae can migrate in soil to find hosts, once roots of the hosts are found, the first instar larvae can dig into plant bodies, the plants are induced to form a plurality of giant cells at feeding sites, then the second instar larvae are molted for three times to develop into adults with reproductive capacity, and the plants also generate root knots. Therefore, the control window period of the root knot nematode disease is mainly focused on the period that second-instar nematodes migrate in soil to search for hosts, and once the nematodes enter the hosts, artificial measures can hardly be taken to kill the nematodes and simultaneously protect plants from being damaged. The control window period is short, generally within two weeks, which also increases the difficulty of root-knot nematode control.
In view of the characteristics of the life history of root-knot nematodes, china advocates a plant protection policy of 'prevention is the main and comprehensive control' for plant nematode diseases. The existing control methods for root-knot nematodes mainly comprise agricultural control, physical control, chemical control, biological control and the like. Wherein the efficiency of agricultural control and physical control is lower, which does not meet the requirement of modern agricultural development; chemical control is the main control means at present, but part of chemical medicines are not friendly to the environment, and the resistance problem of the nematodes is becoming serious day by day at present; the biological control is to utilize natural enemies of nematodes or biological natural products with nematicidal activity to control plant nematodes. For example, abamectin has been widely used as a field nematicide; paecilomyces lilacinus can effectively inhibit nematode eggs and a corresponding product is developed; other biocontrol bacteria, such as Arthrobacter oligospora, bacillus, pseudomonas, and other related defense line products are also under development.
The propionamide (CAS number: 79-05-0) is a small molecular substance with the molecular formula of C 3 H 7 NO, physical properties dissolved in water, ethanol, ether and chloroform. Propionamide may be obtained by reacting propionic acid with ammonia.
The application discovers that propionamide has nematicidal activity for the first time.
Disclosure of Invention
The invention aims to provide application of propionamide in preparing nematocidal preparations, in particular application in root-knot nematode pesticides and/or cyst nematode pesticides. The molecular formula of the propionamide is C 3 H 7 NO, molecular weight 73.09. The micromolecule substance has nematicidal activity, and the chemical formula is as follows:
in order to achieve the purpose, the invention adopts the following technical measures:
the application of propionamide in preparing nematocide, the application process includes taking propionamide as main effective component or preparing nematocide preparation from one of main effective components;
in the above application, preferably, the nematode is meloidogyne incognita, meloidogyne javanica or soybean cyst nematode.
In the above application, preferably, the nematicidal agent is prepared by compounding propionamide and betaine or methyl malonic acid, wherein the mass ratio of the propionamide to the betaine or methyl malonic acid is 1-10.
Compared with the prior art, the invention has the following characteristics:
the invention reports the nematicidal activity of the propionamide for the first time, and the preparation provides a new resource for the research and development of a novel plant nematicidal preparation.
The nematicide prepared from the propionamide has the advantages of high efficiency, low toxicity and good persistence, and has prevention effect on a plurality of plant parasitic nematodes with serious harm.
After the propionamide and the betaine or the methylmalonic acid are compounded, the insecticidal capacity of killing nematodes is remarkably improved, and the propionamide and the betaine or the methylmalonic acid have a synergistic effect.
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FIG. 1 is a graph showing the effect of propionamide in killing Meloidogyne incognita;
in the figure, A is the effect of 200 mu g/mL of propionamide on nematodes for 48 h; b is nematode in the blank.
Detailed Description
The experimental procedures in the following examples are reported to be routine in microbiology unless otherwise specified, and the reagents or materials, unless otherwise specified, are commercially available.
Example 1:
the application of propionamide in preparing a preparation for killing meloidogyne incognita comprises the following steps:
picking nematode egg blocks from tomato roots infected with meloidogyne incognita, placing the egg blocks in a culture dish containing 15mL of sterile water, incubating in darkness at 20 ℃, and observing the incubation condition of the meloidogyne incognita. The nematodes were collected by centrifugation and the nematode concentration was calculated.
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The propionamide of the company is prepared into a 1mg/mL aqueous solution, added into a 96-well plate, and added with sterile water to ensure that the final concentration of the propionamide is 200, 100, 50, 25, 12.5,6.25 mu g/mL in sequence, 30-40 nematodes are added into each well, the total system of the bioassay is 100 mu L, and sterile water is used as a negative control, and each group is repeated for 3 times. And (3) performing microscopic examination to count the mortality of the nematodes after culturing in an incubator at 20 ℃ for 48 hours in the dark, and adding 1 mu L of 5M NaOH solution into a 96-well plate before microscopic examination, wherein dead nematodes are obtained when the nematodes are stiff and straight, and live nematodes are obtained when the nematodes are bent and twisted as shown in figure 1. The activity of propionamide against Meloidogyne incognita is shown in Table 1, LC 50 It was 22.87. Mu.g/mL.
TABLE 1 insecticidal Activity of propionamides against Meloidogyne incognita
Using the above method, applicants have tested the reported insecticidal activity of the nematicidal agents betaine and methylmalonic acid, tested the LC of Meloidogyne incognita by betaine and methylmalonic acid 50 124.54. Mu.g/mL and 28.04. Mu.g/mL, respectively.
Example 2:
the application of propionamide in preparing a preparation for killing meloidogyne javanica comprises the following steps:
picking nematode egg blocks from the roots of tomatoes infected with the meloidogyne javanica, placing the egg blocks in a culture dish containing 15mL of sterile water, incubating the egg blocks in darkness at 20 ℃, and observing the incubation condition of the meloidogyne javanica. The nematodes were collected by centrifugation and the nematode concentration was calculated.
Adding 1mg/mL of propionamide aqueous solution into a 96-well plate to ensure that the final concentration of propionamide is 400, 200, 100, 50, 25 and 12.5 mu g/mL in sequence, adding 30-40 nematodes into each well, wherein the total weight of the bioassay system is 100 mu L, and taking sterile water as a negative control, and repeating each group for 3 times. Performing dark culture in 20 deg.C incubator for 48 hr, performing microscopic examination to obtain statistic of nematode mortality, and determining the activity of propionamide on Meloidogyne javanicus as shown in Table 2, wherein LC 50 It was 107.02. Mu.g/mL.
TABLE 2 insecticidal Activity of propionamides against meloidogyne javanica
Example 3:
the application of propionamide in preparing a preparation for killing soybean cyst nematodes comprises the following steps:
picking nematode cysts from soybean roots infected with soybean cyst nematodes (Heterodera glycines), scratching the cysts to release eggs, placing the cysts in a culture dish containing 15mL of sterile water, incubating in the dark at 20 ℃, and observing the incubation condition of the root-knot nematodes. The nematodes were collected by centrifugation and the nematode concentration was calculated.
Adding 1mg/mL of propionamide aqueous solution into a 96-well plate to ensure that the final concentration of propionamide is 400, 200, 100, 50, 25 and 12.5 mu g/mL in sequence, adding 30-40 nematodes into each well, wherein the total weight of the bioassay system is 100 mu L, and taking sterile water as a negative control, and repeating each group for 3 times. Performing microscopic examination to count the mortality of nematodes after culturing in 20 deg.C incubator for 48 hr, wherein the activity of propionamide on soybean cyst nematodes is shown in Table 3, and LC 50 It was 46.09. Mu.g/mL.
TABLE 3 insecticidal Activity of propionamide against Soybean cyst nematodes
Example 4:
the application of propionamide in preparing a preparation for killing putrescent stem nematodes comprises the following steps:
mixed age nematodes are migrated into water by the tray method from sweet potatoes infected with the nematode canker (Ditylenchus destructor, another plant nematode that is harmful to potatoes, sweet potatoes). The nematodes were collected by centrifugation and the nematode concentration was calculated.
Adding 1mg/mL of propionamide aqueous solution into a 96-well plate to ensure that the final concentration of propionamide is 800, 400, 200, 100, 50 and 25 mu g/mL in sequence, adding 30-40 head nematodes into each well, wherein the total weight of the assay is 100 mu L, and taking sterile water as a negative control, and repeating each group for 3 times. Performing microscopic examination to count the mortality rate of nematodes after culturing in 20 deg.C incubator for 48 hr, wherein the activity of propionamide on rot stem nematodes is shown in Table 4, and LC 50 167.38mg/mL, the activity is lower.
TABLE 4 insecticidal Activity of propionamides against rot stem nematodes
Example 5:
the application of propionamide in preparing a caenorhabditis elegans killing preparation comprises the following application processes:
nematodes were eluted from a petri dish containing larvae of 4 th-instar Caenorhabditis elegans (Caenorhabditis elegans) using M9 buffer, collected by centrifugation, and the nematode concentration was calculated.
Adding 500 mu g/mL of propionamide aqueous solution into a 96-well plate, adding sterile water to ensure that the final concentration of propionamide is 400, 200, 100, 50, 25 and 12.5 mu g/mL in sequence, adding 30-40 nematodes into each well, wherein the total system of the bioassay is 100 mu L, and taking the sterile water as a negative control, and repeating each group for 3 times. After being placed in an incubator at 20 ℃ for dark culture for 48 hours, the mortality of the nematode is counted by microscopic examination, the activity of the propionamide to the caenorhabditis elegans is shown in Table 5, and the LC50 is 21760.91mg/mL.
TABLE 5 insecticidal Activity of propionamides against caenorhabditis elegans
Example 6:
the application of propionamide compounded betaine in preparing a preparation for killing meloidogyne incognita comprises the following application processes:
picking nematode egg blocks from tomato roots infected with meloidogyne incognita, placing the egg blocks in a culture dish containing 15mL of sterile water, incubating in darkness at 20 ℃, and observing the incubation condition of the meloidogyne incognita. The nematodes were collected by centrifugation and the nematode concentration was calculated.
Adding 1mg/mL of a propionamide and betaine compound aqueous solution (the mass ratio is 1:1) into a 96-well plate, adding sterile water to enable the final concentration of the compound solution to be 200, 100, 50, 25 and 12.5,6.25 mu g/mL in sequence, adding 30-40 nematodes into each hole, taking the biological test total system as 100 mu L, taking the sterile water as negative control, and repeating each group for 3 times. Performing microscopic examination to count the mortality of nematodes after culturing in an incubator at 20 ℃ for 48h in the dark, and treating the southern root knot with a propionamide and betaine compound aqueous solution (the mass ratio is 1:1)Nematode Activity as shown in Table 6, LC 50 The activity of the two substances to the root-knot nematode is shown as synergistic effect after being compounded with each other, and the content of the two substances is 13.92 mu g/mL.
TABLE 6 insecticidal Activity of propionamide Complex betaine against Meloidogyne incognita
According to the method, the LC of the propionamide and betaine compounded aqueous solution (the mass ratio is 5:1) to the meloidogyne incognita is measured 50 Is 22.04 mu g/mL, and the LC of the propionamide and betaine compound aqueous solution (the mass ratio is 1:5) to the meloidogyne incognita 50 Is 45.61 mu g/mL, and the LC of a propionamide and betaine compound aqueous solution (the mass ratio is 10 50 20.07 mu g/mL, and the LC of a propionamide and betaine compound aqueous solution (mass ratio of 1 50 The activity of the two substances to the root-knot nematode is shown as synergistic effect after the two substances are compounded according to the proportion.
Example 7:
the application of propionamide compounded with methylmalonic acid in preparing the preparation for killing meloidogyne incognita comprises the following application processes:
picking nematode egg blocks from tomato roots infected with meloidogyne incognita, placing the egg blocks in a culture dish containing 15mL of sterile water, incubating in darkness at 20 ℃, and observing the incubation condition of the meloidogyne incognita. The nematodes were collected by centrifugation and the nematode concentration was calculated.
Adding 1mg/mL of propionamide and methylmalonic acid compound aqueous solution (the mass ratio is 1:1) into a 96-well plate, adding sterile water to enable the final concentration of the compound solution to be 200, 100, 50, 25 and 12.5,6.25 mu g/mL in sequence, adding 30-40 nematodes into each well, taking the biological total system as 100 mu L, taking the sterile water as negative control, and repeating each group for 3 times. Performing microscopic examination to count the mortality of the nematodes after culturing in an incubator at 20 ℃ for 48h in the dark, wherein the activity of the propionamide and methylmalonic acid compound aqueous solution (the mass ratio is 1:1) on the meloidogyne incognita is shown in Table 7, and LC 50 22.56 mu g/mL, and the activity of the two substances on the root-knot nematode shows synergy after being compoundedAnd (5) synergism is achieved.
TABLE 7 insecticidal Activity of propionamide Complex Methylmalonic acid against Meloidogyne incognita
According to the method, the LC of the propionamide and methyl malonic acid compound aqueous solution (the mass ratio is 5:1) to meloidogyne incognita is measured 50 Is 18.20 mu g/mL, and the LC of the compounded aqueous solution of the propionamide and the methylmalonic acid (the mass ratio is 1:5) to the meloidogyne incognita 50 Is 21.44 mu g/mL, the LC of the compound aqueous solution of propionamide and methylmalonic acid (the mass ratio is 10 50 14.48 mu g/mL, and the LC of a compounded aqueous solution of propionamide and methylmalonic acid (the mass ratio is 1 50 The activity of the two substances to the root-knot nematode is shown as synergistic effect after the two substances are compounded according to the proportion.
Claims (3)
1. The application of propionamide in preparing nematode pesticide is disclosed, and the nematode is Meloidogyne incognita, meloidogyne javanicus and Soy cyst nematode.
2. The nematode pesticide consists of propionamide and betaine, and the mass ratio is 1 to 10.
3. The nematode pesticide consists of propionamide and methylmalonic acid, and the mass ratio is 1 to 10.
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| CN107136074A (en) * | 2017-05-17 | 2017-09-08 | 中国农业大学 | Potassium diformate prevents and treats the new application of Meloidogyne incognita |
| CN108935466B (en) * | 2018-07-11 | 2020-06-09 | 湖北省生物农药工程研究中心 | Application of methylmalonic acid in preparation of nematode pesticide |
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