CN114885948B - Nematicidal composition for preventing and treating taro root knot nematode disease - Google Patents
Nematicidal composition for preventing and treating taro root knot nematode disease Download PDFInfo
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- CN114885948B CN114885948B CN202210695406.0A CN202210695406A CN114885948B CN 114885948 B CN114885948 B CN 114885948B CN 202210695406 A CN202210695406 A CN 202210695406A CN 114885948 B CN114885948 B CN 114885948B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention relates to the technical field of pesticides, and particularly relates to a nematode killing composition for preventing and treating taro root knot nematode disease. The effective components of the nematicidal composition for preventing and treating taro root knot nematode disease are compounded by lentinan, cyflumetofen, sulfoxaflor or thiocyclam; wherein the mass ratio of the lentinan to the cyflumetofen, the sulfoxaflor or the thiocyclam is 1-100:100-1. The prevention and control of the meloidogyne incognita is shown as a synergistic effect after the lentinan, the cyflumetofen, the sulfoxaflor or the thiocyclam in the nematicidal composition are compounded, compared with a single effective component, the prevention and control effect of the meloidogyne incognita can be effectively improved, and a support can be provided for research and development of a compound agent for preventing and controlling the meloidogyne incognita.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a nematode killing composition for preventing and treating taro root knot nematode disease.
Background
Plant nematode disease is an important disease species in world agricultural production, with losses caused by root-knot nematodes accounting for over 50%. The root-knot nematode has wide host, can infect more than 3000 plants, contains most crops, and generally can cause the yield reduction of the crops by 10-20 percent, and can reach more than 75 percent in severe cases. More than 90% of plant root knot nematode diseases are reported to be caused by 4 nematodes, such as southern root knot nematode (Meloidogyne incognita), northern root knot nematode (Meloidogyne hapla), peanut root knot nematode (Meloidogyne arenaria), and Java root knot nematode (Meloidogyne javanica). The root-knot nematode exists mainly in soil with the depth of 5-30cm, destroys the root system structure of plants by invading wounds and forming nodose root knots, absorbs nutrient substances, influences the normal metabolic activity of the plants, and causes the consistent infection of other pathogenic bacteria to cause the reduction and even death of the growth vigor of the plants, thereby reducing the yield of crops and even preventing the crops from being harvested.
The taro is damaged by root-knot nematodes in the planting process, and the yield and the quality of the taro are influenced. At present, the chemical agent is a main means for preventing and treating the meloidogyne hapla disease of the taro field, and has the advantages of quick acting, stability, lower cost and the like. Due to the accumulation of drug resistance of the root-knot nematodes, the single chemical agent has effective drug effect under the conventional dosage, so that the research and development of a novel compound agent for preventing and treating the root-knot nematode disease are necessary.
Lentinan (Lentinan) is an effective active ingredient extracted from fruiting body of high-quality Lentinus edodes, and is an immunopotentiator. Clinical and pharmacological research shows that lentinan has the functions of resisting virus, resisting tumor, regulating immunity, stimulating the formation of interferon, etc. Application number CN201510172220.7 discloses a nematicidal and disease-resistant composition and application thereof, and specifically discloses a combination of fulvene linear sulfone and lentinan at 80:1-1:80, the co-toxicity coefficient CTC of the cucumber root-knot nematode combined toxicity test is more than 120, and the synergistic effect is realized.
The inventor discovers that the compound of lentinan and cyflumetofen, sulfoxaflor or thiocyclam shows synergistic effect on the control of root-knot nematodes through indoor tests.
At present, no report related to the compounding of lentinan and cyflumetofen, sulfoxaflor or thiocyclam is found.
Disclosure of Invention
The invention aims to provide a nematicidal composition for preventing and treating taro root-knot nematode, wherein two effective components of the nematicidal composition show a synergistic effect on the prevention and treatment of the root-knot nematode, can improve the prevention and treatment effect on the root-knot nematode, effectively prevent and treat the taro root-knot nematode, and can provide support for research and development of a compound agent for preventing and treating the root-knot nematode.
In order to achieve the purpose, the invention provides the following technical scheme:
the effective components of the nematicidal composition for preventing and treating taro root knot nematode disease are compounded by lentinan, cyflumetofen, sulfoxaflor or thiocyclam; wherein the mass ratio of the lentinan to the cyflumetofen, the sulfoxaflor or the thiocyclam is 1-100:100-1.
Preferably, the mass ratio of the lentinan to the cyflumetofen is 1-60:27-1.
Preferably, the mass ratio of the lentinan to the sulfoxaflor is 1:30-5.
Preferably, the mass ratio of the lentinan to the thiocyclam is 1-7:4-1.
Compared with the prior art, the invention has the following beneficial effects:
the composition for killing nematodes has a synergistic effect on the control of southern root-knot nematodes after compounding lentinan, cyflumetofen, sulfoxaflor or thiocyclam, can effectively improve the control effect on taro root-knot nematodes compared with a single active ingredient, and can provide support for research and development of a compound agent for controlling taro root-knot nematodes.
Detailed Description
The present invention will be better understood from the following examples, which are given for illustrative purposes only and should not be construed as limiting the invention as detailed in the claims.
Examples: indoor bioactivity test of lentinan compound
1. Test subjects: meloidogyne incognita (Meloidogyne incognita)
2. Reagent for testing
96% lentinan active compound (Shanghai Yuan leaf Biotech Co., ltd.)
95% cyflumetofen original drug (Shanghai Yuan leaf biotechnology limited)
99% sulfoxaflor (Jiangsu Yiheng pharmaceutical Co., ltd.)
90% thiocyclam original drug (Heilongjiang Jixiang agriculture Co., ltd.)
Dissolving the raw medicine with dimethyl sulfoxide to prepare single-dose mother liquor, diluting with 0.1% Tween-80 aqueous solution, setting multiple groups of ratios, setting 7 gradient mass concentrations for each single dose and each group of ratio mixture according to an equal ratio method, and setting 50mL of liquid medicine with each mass concentration for later use.
3. The test method comprises the following steps: (refer to NT/Y1833.1-2009 Standard of indoor bioassay of pesticides test rules nematocide part 1 test of nematode inhibition on plant pathogenic nematodes)
And (3) selecting eggs of root-knot nematodes from the roots of taros, cleaning the eggs with clear water, placing the eggs on wet filter paper of a culture dish, and incubating the eggs at 25 ℃ to obtain the second-instar larvae with consistent instars. Eluting the cultured root-knot nematodes with water, filtering, centrifuging at 1000r/min for 2min, discarding the supernatant, adding water, centrifuging again, and finally resuspending the root-knot nematodes with water to 400 heads/mL for later use. And sequentially sucking 3mL of liquid medicine into the test tubes from low concentration to high concentration by using a pipette, and then sucking the prepared equal-amount nematode suspension into the test tubes to uniformly mix the liquid medicine and the nematode suspension in equal amount. Moving a certain volume of the mixed solution into the small hole of the biochemical test plate with 24 holes by a liquid moving machine, covering the small hole, and culturing at the constant temperature of 25 ℃ for 24 hours. Each treatment was repeated 4 times, and treatments containing dimethyl sulfoxide and 0.1% tween-80 in water were used as controls. 1mL of mixed solution is taken from each process, the death condition of the nematodes is observed under an anatomical lens, the number of the nematodes is not less than 100 when the nematodes are observed repeatedly, and the number of the total nematodes and the number of the dead nematodes are recorded. The determination standard of nematode death is as follows: the nematodes are stiff and still cannot move in a bending manner when touched by a hair pin or a bamboo pin. Mortality and corrected mortality for each treatment were calculated from the survey data.
In the above formula: p- -mortality in%; k- -number of dead insects; n- -total number of treated worms.
In the above formula: p 1 Corrected mortality in%; p t Treatment mortality in%; p is 0 Control mortality in%.
4. And (3) data analysis: using DPS software to perform regression analysis on the log values of each treatment agent concentration and the corrected mortality probability value of each treatment, and calculating LC of each treatment agent 50 And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.
In the above formula: ATI- -the observed virulence index of the combination; s- -LC of Standard drug 50 In mg/L; m-LC of test agent 50 The unit is mg/L.
TTI＝TI A ×P A +TI B ×P B
In the above formula: TTI- -theoretical toxicological index of the compound; TI A -the virulence index of the agent a; p A -the percentage of agent a in the mixture in percent (%); TI B -virulence index of the B agent; p B The percentage of the agent B in the mixture is given in percentage (%).
In the above formula: CTC-co-toxicity coefficient; ATI- -measured virulence index of the mixture; TTI- -mixture theory virulence index.
5. Evaluation of drug efficacy
And evaluating the synergism of the medicament according to the calculated co-toxicity coefficient (CTC), wherein the antagonism is the CTC of less than or equal to 80, the additivity is the CTC of more than 80 and less than 120, and the synergism is the CTC of more than or equal to 120, and the results are shown in tables 1-3.
TABLE 1 measurement result of toxicity of compounded lentinan and cyflumetofen to Meloidogyne incognita
| Medicament | Mass ratio of | LC 50 (mg/L) | ATI | TTI | CTC |
| Lentinan | -- | 63.23 | 100.00 | -- | -- |
| Cyflumetofen | -- | 7.59 | 833.07 | -- | -- |
| Lentinan: cyflumetofen | 1：27 | 6.25 | 1011.68 | 806.89 | 125.38 |
| Lentinan: cyflumetofen | 1：15 | 4.41 | 1433.79 | 787.25 | 182.13 |
| Lentinan: cyflumetofen | 1：7 | 3.87 | 1633.85 | 741.44 | 220.36 |
| Lentinan: cyflumetofen | 1：5 | 6.59 | 959.48 | 710.89 | 134.97 |
| Lentinan: cyflumetofen | 1：1 | 9.01 | 701.78 | 466.53 | 150.42 |
| Lentinan: cyflumetofen | 5：1 | 10.82 | 584.38 | 222.18 | 263.02 |
| Lentinan: cyflumetofen | 7：1 | 13.83 | 457.19 | 191.63 | 238.58 |
| Lentinan: cyflumetofen | 15：1 | 24.74 | 255.58 | 145.82 | 175.27 |
| Lentinan: tetrafluminellaEsters | 30：1 | 27.17 | 232.72 | 123.65 | 188.21 |
| Lentinan: cyflumetofen | 60：1 | 42.70 | 148.08 | 112.02 | 132.19 |
As can be seen from Table 1, in the range of 1-60:27-1, the co-toxicity coefficient of the compounded lentinan and cyflumetofen to the meloidogyne incognita is more than 120, and the synergistic effect is shown.
TABLE 2 virulence determination results of the combination of lentinan and sulfoxaflor against Meloidogyne incognita
| Medicament | Mass ratio of | LC 50 (mg/L) | ATI | TTI | CTC |
| Lentinan | -- | 63.23 | 100.00 | -- | -- |
| Sulfoxaflor | -- | 6.62 | 955.14 | -- | -- |
| Lentinan: sulfoxaflor | 1：30 | 3.01 | 2100.66 | 927.55 | 226.47 |
| Lentinan: sulfoxaflor | 1：15 | 5.56 | 1137.23 | 901.69 | 126.12 |
| Lentinan: sulfoxaflor | 1：7 | 2.65 | 2386.04 | 848.24 | 281.29 |
| Mushroom (Lentinus edodes)Polysaccharide: sulfoxaflor | 1：5 | 4.83 | 1309.11 | 812.61 | 161.10 |
As can be seen from table 1, in 1: within the mass ratio range of 30-5, the co-toxicity coefficient of the compounded lentinan and sulfoxaflor to Meloidogyne incognita is more than 120, and the synergistic effect is shown.
TABLE 3 virulence determination results of Meloidogyne incognita by compounding lentinan and thiocyclam
| Medicament | Mass ratio of | LC 50 (mg/L) | ATI | TTI | CTC |
| Lentinan | -- | 63.23 | 100.00 | -- | -- |
| Insecticidal ring | -- | 18.92 | 334.20 | -- | -- |
| Lentinan: insecticidal ring | 1：4 | 16.53 | 382.52 | 287.36 | 133.12 |
| Lentinan: insecticidal ring | 1：2 | 13.80 | 458.19 | 256.13 | 178.89 |
| Lentinan: insecticidal ring | 1：1 | 21.93 | 288.33 | 217.10 | 132.81 |
| Lentinan: insecticidal ring | 3：1 | 15.34 | 412.19 | 158.55 | 259.98 |
| Lentinan: killing-out deviceInsect ring | 5：1 | 22.72 | 278.30 | 139.03 | 200.17 |
| Lentinan: insecticidal ring | 7：1 | 14.58 | 433.68 | 129.27 | 335.47 |
As can be seen from Table 4, in the range of 1-7:4-1, the co-toxicity coefficient of the compounded lentinan and thiocyclam to the meloidogyne incognita is more than 120, and the synergistic effect is shown.
In conclusion, the biological activity of the lentinan compounded with the cyflumetofen, the sulfoxaflor or the thiocyclam shows a synergistic effect on the meloidogyne incognita, and compared with a single effective component, the composition can effectively improve the control effect on the taro meloidogyne incognita.
The foregoing is only a preferred embodiment of the present invention and modifications, which are within the scope of the invention, will be apparent to those skilled in the art without departing from the principles of the invention.
Claims (1)
1. A nematicidal composition for preventing and treating taro root knot nematode disease is characterized in that the effective component is compounded by lentinan and cyflumetofen; the mass ratio of the lentinan to the cyflumetofen is 1-60:27-1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310249496.5A CN116326585B (en) | 2022-06-20 | 2022-06-20 | Nematicide composition for improving prevention and control effect of taro root knot nematode disease |
| CN202310249497.XA CN116138257B (en) | 2022-06-20 | 2022-06-20 | Nematicide composition containing lentinan for preventing and treating taro root knot nematode disease |
| CN202210695406.0A CN114885948B (en) | 2022-06-20 | 2022-06-20 | Nematicidal composition for preventing and treating taro root knot nematode disease |
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| CN202310249497.XA Division CN116138257B (en) | 2022-06-20 | 2022-06-20 | Nematicide composition containing lentinan for preventing and treating taro root knot nematode disease |
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| CN106135230B (en) * | 2015-04-13 | 2018-05-15 | 深圳诺普信农化股份有限公司 | A kind of nematicidal disease-resistant composition and its application |
| CN104770389B (en) * | 2015-04-16 | 2018-02-16 | 广东中迅农科股份有限公司 | A kind of composition pesticide for being used to prevent and treat root-knot nematode |
| CN106912494A (en) * | 2017-03-14 | 2017-07-04 | 山东省联合农药工业有限公司 | A kind of plant vaccine of phosphorous worm prestige and sandworm toxin insecticide and preparation method thereof |
| CN106973937B (en) * | 2017-05-12 | 2019-12-31 | 广东真格生物科技有限公司 | Nematicidal composition containing cyflumetofen |
| CN110384096A (en) * | 2019-08-06 | 2019-10-29 | 京博农化科技有限公司 | A kind of composition pesticide containing flonicamid and lentinan |
| CN111296449B (en) * | 2020-03-13 | 2021-08-27 | 杭州市农业科学研究院 | Application of stropharia rugoso-annulata polysaccharide, resistance inducer and grape cultivation method |
| CN114868761B (en) * | 2022-04-11 | 2023-05-30 | 江西新龙生物科技股份有限公司 | Bactericide for preventing and treating citrus yellow dragon disease and application thereof |
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