CN116250546A - Composition for controlling grassland locust insect pests - Google Patents
Composition for controlling grassland locust insect pests Download PDFInfo
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- CN116250546A CN116250546A CN202211700337.4A CN202211700337A CN116250546A CN 116250546 A CN116250546 A CN 116250546A CN 202211700337 A CN202211700337 A CN 202211700337A CN 116250546 A CN116250546 A CN 116250546A
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- parts
- destruxin
- composition
- locust
- spore powder
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- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 37
- 239000000203 mixture Substances 0.000 title claims abstract description 30
- 241000238631 Hexapoda Species 0.000 title abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 32
- 239000005660 Abamectin Substances 0.000 claims abstract description 28
- 239000000284 extract Substances 0.000 claims abstract description 26
- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 claims abstract description 21
- 229950008167 abamectin Drugs 0.000 claims abstract description 21
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 241000208250 Calotropis gigantea Species 0.000 claims abstract 8
- 241000223250 Metarhizium anisopliae Species 0.000 claims description 19
- 239000004480 active ingredient Substances 0.000 claims description 7
- 239000012530 fluid Substances 0.000 claims description 6
- RRZXIRBKKLTSOM-XPNPUAGNSA-N avermectin B1a Chemical compound C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 RRZXIRBKKLTSOM-XPNPUAGNSA-N 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 10
- 239000000575 pesticide Substances 0.000 abstract description 9
- 244000241257 Cucumis melo Species 0.000 abstract description 5
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 abstract description 5
- 244000144977 poultry Species 0.000 abstract description 5
- 230000002195 synergetic effect Effects 0.000 abstract description 5
- 238000013329 compounding Methods 0.000 abstract description 2
- 230000002147 killing effect Effects 0.000 abstract description 2
- 241001247986 Calotropis procera Species 0.000 description 21
- 241000238814 Orthoptera Species 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 230000002265 prevention Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- NXCPMSUBVRGTSE-UHFFFAOYSA-N 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-1-one Chemical compound COC1=CC(C(=O)CCO)=CC=C1O NXCPMSUBVRGTSE-UHFFFAOYSA-N 0.000 description 2
- RGHHSNMVTDWUBI-UHFFFAOYSA-N 4-hydroxybenzaldehyde Chemical compound OC1=CC=C(C=O)C=C1 RGHHSNMVTDWUBI-UHFFFAOYSA-N 0.000 description 2
- 241000254032 Acrididae Species 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 2
- 241000208327 Apocynaceae Species 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000243190 Microsporidia Species 0.000 description 2
- ZQMCMXRXLGCJJJ-MRVPVSSYSA-N O=C(OC)[C@H](CO)c1cc(OC)c(O)cc1 Chemical compound O=C(OC)[C@H](CO)c1cc(OC)c(O)cc1 ZQMCMXRXLGCJJJ-MRVPVSSYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 239000000463 material Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- UTXNRISXYKZJTH-UHFFFAOYSA-N 2,3-Dihydroxy-1-(4-hydroxy-3-methoxyphenyl)-1-propanone Chemical compound COC1=CC(C(=O)C(O)CO)=CC=C1O UTXNRISXYKZJTH-UHFFFAOYSA-N 0.000 description 1
- REZVXIYYURPOSL-UHFFFAOYSA-N 9alpha-hydroxypinoresinol Natural products C1=C(O)C(OC)=CC(C2C3C(O)OC(C3CO2)C=2C=C(OC)C(O)=CC=2)=C1 REZVXIYYURPOSL-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- GIDCYTNGBLUMQG-MRVPVSSYSA-N C-veratroylglycol Natural products OC[C@@H](O)C(=O)c1ccc(O)cc1 GIDCYTNGBLUMQG-MRVPVSSYSA-N 0.000 description 1
- 241000227752 Chaetoceros Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000005949 Malathion Substances 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 235000003283 Pachira macrocarpa Nutrition 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000270708 Testudinidae Species 0.000 description 1
- 240000001085 Trapa natans Species 0.000 description 1
- 235000014364 Trapa natans Nutrition 0.000 description 1
- JPBXWMPAUUZPQK-UHFFFAOYSA-N Veratroylaethylenglykol Natural products COC1=CC=C(C(=O)C(O)CO)C=C1OC JPBXWMPAUUZPQK-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
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- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- JLQUFIHWVLZVTJ-UHFFFAOYSA-N carbosulfan Chemical compound CCCCN(CCCC)SN(C)C(=O)OC1=CC=CC2=C1OC(C)(C)C2 JLQUFIHWVLZVTJ-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
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- 239000010779 crude oil Substances 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- JXSJBGJIGXNWCI-UHFFFAOYSA-N diethyl 2-[(dimethoxyphosphorothioyl)thio]succinate Chemical compound CCOC(=O)CC(SP(=S)(OC)OC)C(=O)OCC JXSJBGJIGXNWCI-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000000967 entomopathogenic effect Effects 0.000 description 1
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 229940041033 macrolides Drugs 0.000 description 1
- 229960000453 malathion Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007830 nerve conduction Effects 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- CSQGINGXEBQJPA-UHFFFAOYSA-N omega-hydroxypropioguaiacone Natural products COC1=CC(CC(=O)CO)=CC=C1O CSQGINGXEBQJPA-UHFFFAOYSA-N 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009165 saligot Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Dentistry (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Virology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Insects & Arthropods (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticide compounding, and relates to a composition for grassland locust pest control, which comprises, by mass, 155-480 parts of destruxin spore powder, 130-350 parts of abamectin and 8-27 parts of root extract of Calotropis gigantea; the destruxin spore powder is derived from destruxin IPPMHBHC-7, and the preservation number of the destruxin IPPMHBHC-7 is CGMCC No.15999. After the destruxin spore powder, abamectin and the root extract of the white flower cantaloupe are compounded, the invention finds that the destruxin spore powder, abamectin and white flower cantaloupe are excellent in locust control and show a synergistic effect. The composition provided by the invention has high trapping and killing effects and good control effects, and is suitable for controlling grassland locust and insect pests by matching with a grassland locust control method of poultry.
Description
Technical Field
The invention belongs to the technical field of pesticide compounding, and relates to a composition for grassland locust pest control.
Background
The grassland is the land ecological system with the largest area in China, and is an important material foundation for the development of animal husbandry and basic production and living data for the survival of farmers and herding citizens. In recent years, insect pests are gradually one of the most frequent and serious biological disasters on grasslands under the influence of global climate change, unreasonable utilization and other factors. Among them, the most serious grasshopper disaster, flood disaster, drought disaster and three natural disasters are called as the same. The grassland pests gnaw a large amount of pasture, seriously destroy grassland vegetation, and seriously threaten national ecological safety, animal husbandry production and farmer and herd life. Specific species of grassland locust include: bai Wenwei locusts, willd's locust, japanese water chestnut, asiatic grasshopper, etc.
The metarhizium anisopliae (Metarhizium anisopliae) is an important entomopathogenic fungus, and the preparation taking the metarhizium anisopliae as a main bioactive component has the advantages of no residue, easy production, long lasting period, strong pathogenicity, good application effect, safety to non-target organisms and the like. Metarhizium Anisopliae has been widely used for biological pest control since the first control of australia tortoise (Anisopliae aus-triac). In grassland insect pest prevention and control work, the metarhizium anisopliae is mainly used for grassland locust prevention and control. CN109401981a discloses metarhizium anisopliae IPPMHBHC-7 and application thereof, metarhizium anisopliae (Metarhizium anisopliae) IPPMHBHC-7 has a preservation number of CGMCC No.15999, and has good half-killing time and stiff insect rate for Asiatic dolly locusts and Chaetoceros mandshurica.
Avermectins (AVMs), which are the most interesting insecticidal agents at home and abroad, are sixteen-membered ring macrolides, were developed successfully by Merck corporation in the United states at the earliest, and can inhibit invertebrate nerve conduction substances to cause paralysis and death of insects. With the increase of the application amount of chemical pesticides and the increase of the prevention frequency, the locusts can generate drug resistance, so that the prevention effect is reduced. On the premise of continuously enhancing the ecological environment protection, various biopesticides are also used for grassland locust control, and play an important role in protecting the biological diversity of the grassland ecological system and improving the natural disaster resistance of the ecological system.
Calotropis gigantea Calotropisprocera (Aiton) W.T.Aiton is a plant of Calotropis gigantea of Asclepiadaceae. The prior literature has 8 compounds separated from the root of the plant Calotropis gigantea of the Asclepiadaceae, which are identified as 1-O-methyl-guaiacyl glyco-hol, omega-hydroxypropioguaiacone, C-Veratroylglycol, ficusol, (+) -eugenol, 9 alpha-hydroxy pinoresinol, p-hydroxybenzaldehyde and niacinamide by spectral analysis, which are all first separated from the compounds.
The main methods adopted by locust control at present are chemical control and biological control, and in the practical application process, a strategy that a plurality of control means are commonly used is often adopted. The chemical prevention and treatment means mainly comprise: spraying chemical agents such as carbosulfan, malathion crude oil and the like or trapping by using poison bait. The biological control mainly comprises the following measures: (1) In the locust disaster area, high-efficiency low-toxicity pesticides and biological pesticides are used for protecting predatory natural enemies in the locust area. Can also be used for preventing and controlling fowl such as chicken, duck, goose, etc. by breeding on hillside. (2) Large area application of microsporidia. The method has certain defects, has certain toxicity in chemical control and can pollute the environment; although the biological control does not cause pollution, the searching of effective biological pesticides is very difficult, and the method for stocking poultry is not preferable in some grassland areas with severe natural environments; the large area application of microsporidia may kill some beneficial natural enemies of pests, causing ecological imbalance. Thus, it is desirable to provide a composition for grassland locust pest control that combines the advantages of chemical control means and biological control, improves the grassland locust pest control effect, and reduces the interaction between chemical control and biological control.
Disclosure of Invention
The invention aims to provide a composition for grassland locust pest control, which combines the advantages of chemical control means and biological control, improves the grassland locust pest control effect, and reduces the interaction between chemical control and biological control.
Based on this, the present invention provides a composition for grassland locust pest control to meet this need in the art. The active ingredients of the composition comprise, by mass: 155-480 parts of destruxin spore powder, 130-350 parts of abamectin and 8-27 parts of root extract of white flower calotropis gigantea; the destruxin spore powder is derived from destruxin IPPMHBHC-7, and the preservation number of the destruxin IPPMHBHC-7 is CGMCC No.15999.
Those skilled in the art easily know that the pesticide effect of the compound pesticide is improved, the occurrence of drug resistance is avoided, the insecticidal spectrum is expanded, and a plurality of pesticides are combined or used in combination, but whether the insecticidal effect after the combined or combined use of the pesticides is synergistic or not is difficult to expect because the inherent mechanism of each component is unknown. The inventor team of the patent researches through intensive experiments, and comprehensively evaluates the effect shown by toxicity measurement, and the composition provided by the invention shows excellent control effect on locusts and is better than the use effect of single dose.
In a preferred embodiment, the composition for grassland locust pest control provided by the invention comprises, by mass, 270-390 parts of destruxin spore powder, 215-290 parts of abamectin and 16-20 parts of root extract of Calotropis gigantea.
In a more preferred embodiment, the composition for grassland locust pest control provided by the invention comprises the active ingredients of 370 parts of destruxin spore powder, 240 parts of avermectin and 17 parts of root extract of calotropis gigantea.
In a more preferred embodiment, the composition for grassland locust pest control provided by the invention comprises, by mass, 367 parts of destruxin spore powder, 279 parts of avermectin and 17 parts of root extract of Calotropis gigantea.
In a more preferred embodiment, the composition for grassland locust pest control provided by the invention comprises, by mass, 280 parts of destruxin spore powder, 220 parts of abamectin and 19 parts of root extract of Calotropis gigantea.
Further, in the composition for grassland locust pest control provided by the invention, the concentration of the metarhizium anisopliae spore powder is 1 multiplied by 10 8 -5×10 8 Each/g.
Further, in the composition for grassland locust pest control provided by the invention, the root extract of the Calotropis gigantea adopts supercritical CO from the dry root of the Calotropis gigantea 2 Extracting fluid to obtain; the supercritical CO 2 The pressure of the fluid extraction was 28MPa and the temperature was 53 ℃.
Still another object of the present invention is the use of a composition for grassland locust pest control in locust pest control. In particular, the grassland locust comprises an asian small car locust. In addition, the composition for grassland locust pest control provided by the invention is suitable for controlling grassland locust pests by matching with a method for controlling grassland locust by using poultry. The method for controlling the grassland by using the poultry is exemplified by releasing the grassland, raising the chicken and the duck in the period of the locusts and preventing the locusts in the grassland locusts generation area, and increasing the economic income of the herder while preventing and controlling the grassland locusts.
Compared with the prior art, the composition of the invention has at least the following advantages or beneficial effects:
after the destruxin spore powder, abamectin and the root extract of the white flower cantaloupe are compounded, the invention finds that the destruxin spore powder, abamectin and white flower cantaloupe are excellent in locust control and show a synergistic effect. The composition provided by the invention has high trapping and killing effects and good control effects, and is suitable for controlling grassland locust and insect pests by matching with a grassland locust control method of poultry.
Detailed Description
The following describes the technical aspects of the present invention with reference to examples, but the present invention is not limited to the following examples.
The experimental methods and the detection methods in the following embodiments are all conventional methods unless otherwise specified; the medicaments and materials are commercially available unless specified; the index data are all conventional measurement methods unless specified.
The present example provides an indoor toxicity test of a composition for grassland locust pest control.
Preparation of the metarhizium anisopliae spore powder: the metarhizium anisopliae IPPMHBHC-7 (with the preservation number of CGMCC No. 15999) is inoculated on PDA solid culture medium (200 g of potato juice, 20g of agar, 20g of sucrose, 5g of yeast powder, water supplementing and volume fixing to 1000mL, and high-pressure sterilization at 121 ℃ for 25 min) and cultured until spore is produced, bacterial colony and spore form. The spore concentration was 2.5X10 8 The number of the metarhizium anisopliae spores per gram.
Avermectin stock solution (B) 1a > 96.5%) from Zhejiang province, biochemical Co., ltd.
Root extract of Calotropis gigantea is prepared from dried root of Calotropis gigantea Calotropis procera (Aiton) W.T.Aiton by supercritical CO 2 Fluid extraction, supercritical CO 2 The pressure of the fluid extraction was 28MPa and the temperature was 53 ℃.
Single dose group 1: spore concentration was 2.5X10 8 Metarhizium anisopliae spore powder per gram;
single dose group 2: abamectin;
single dose group 3: root extract of Calotropis gigantea;
test group 1: 155 parts of metarhizium anisopliae spore powder, 130 parts of abamectin and 8 parts of root extract of white flower calotropis gigantea;
test group 2: 270 parts of destruxin spore powder, 215 parts of abamectin and 16 parts of root extract of white flower calotropis gigantea;
test group 3: 370 parts of metarhizium anisopliae spore powder, 240 parts of abamectin and 17 parts of root extract of white flower calotropis gigantea;
test group 4: 367 parts of metarhizium anisopliae spore powder, 279 parts of abamectin and 17 parts of root extract of white flower calotropis gigantea;
test group 5: 280 parts of metarhizium anisopliae spore powder, 220 parts of abamectin and 19 parts of root extract of white flower calotropis gigantea;
test group 6: 390 parts of destruxin spore powder, 290 parts of abamectin and 20 parts of root extract of white flower calotropis gigantea;
test group 7: 480 parts of destruxin spore powder, 350 parts of abamectin and 27 parts of root extract of white flower calotropis gigantea.
The virulence effect of the 10 test agents on Asiatic car locusts was determined by the stomach toxicity method. Asian dolly locust (oedaleuseuse decorusasatisicusb.bienko): the 2-3-year-old locusts are collected in inner Mongolian grassland by a net sweeping method. The active ingredients of each test group were diluted to appropriate concentrations with sterile water, 6 test concentrations were set up, 20 Asian vehicle locusts at 3 years of age per concentration, 3 replicates were set up, and blank control was with sterile water. Placing the locusts after oral administration treatment in a special gauze cage, checking after 48h, counting dead insects, performing statistical analysis, and adopting statistical analysis software SPSS 22.0 to obtain LC by correcting mortality value 50 And co-toxicity coefficients. When the co-toxicity coefficient (CTC) is greater than 120, the effective substances have synergistic effect, the co-toxicity coefficient (CTC) is between 80 and 120, the effective substances have additive effect, and the co-toxicity coefficient (CTC) is less than 80, and the effective substances have antagonistic effect. The test results are shown in Table 1.
TABLE 1 cotoxicity coefficient of compositions for grassland locust pest control
As shown in Table 1, after the root extracts of the destruxins of Metarrhizium anisopliae spore powder, the abamectin and the Calotropis gigantea are compounded, the invention has excellent locust control and synergistic effect. Wherein, the co-toxicity coefficient is 124.91-157.88, which shows that the root extracts of the active substances of the destruxin spore powder, the abamectin and the white flower cantaloupe have synergism on locust control after being compounded.
The present invention may be better implemented as described above, and the above examples are merely illustrative of preferred embodiments of the present invention and not intended to limit the scope of the present invention, and various changes and modifications made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection defined by the present invention without departing from the spirit of the design of the present invention.
Claims (8)
1. The composition for grassland locust pest control is characterized by comprising the following active ingredients in parts by mass: 155-480 parts of destruxin spore powder, 130-350 parts of abamectin and 8-27 parts of root extract of white flower calotropis gigantea; the destruxin spore powder is derived from destruxin IPPMHBHC-7, and the preservation number of the destruxin IPPMHBHC-7 is CGMCC No.15999.
2. The composition for grassland locust pest control according to claim 1, wherein the active ingredients comprise, by mass, 270-390 parts of destruxin spore powder, 215-290 parts of abamectin and 16-20 parts of root extract of calotropis gigantea.
3. The composition for grassland locust pest control according to claim 2, wherein the active ingredients comprise, in parts by mass, 370 parts of destruxin spore powder, 240 parts of abamectin and 17 parts of root extract of calotropis gigantea.
4. The composition for grassland locust pest control according to claim 2, wherein the active ingredients comprise, in parts by mass, 367 parts of destruxin spore powder, 279 parts of avermectin and 17 parts of root extract of calotropis gigantea.
5. The composition for grassland locust pest control according to claim 2, wherein the effective components comprise, in parts by mass, 280 parts of destruxins of Metarrhizium anisopliae spore powder, 220 parts of avermectin and 19 parts of root extract of Calotropis gigantea.
6. According to claimThe composition for grassland locust pest control as recited in claim 1, wherein the concentration of the destruxin spore powder is 1X 10 8 -5×10 8 Each/g.
7. A composition for grassland locust pest control as claimed in claim 1, wherein the root extract of the calotropis gigantea is prepared from dry root of calotropis gigantea by supercritical CO 2 Extracting fluid to obtain; the supercritical CO 2 The pressure of the fluid extraction was 28MPa and the temperature was 53 ℃.
8. Use of the composition for grassland locust pest control as claimed in any of claims 1 to 7 for locust pest control.
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