CN111296444A

CN111296444A - Insecticidal composition containing methoprene compound and application thereof

Info

Publication number: CN111296444A
Application number: CN201811514896.XA
Authority: CN
Inventors: 姚志牛; 潘利东
Original assignee: Jiangsu Work Is At Bio Tech Ltd
Current assignee: Jiangsu Work Is At Bio Tech Ltd
Priority date: 2018-12-12
Filing date: 2018-12-12
Publication date: 2020-06-19

Abstract

The invention relates to an insecticidal composition containing methoprene compounds and application thereof, wherein the composition comprises an active component A and an active component B, the active component A is selected from one of (S) -methoprene and methoprene, and the active component B is selected from one of indoxacarb, metaflumizone, spinosad, spinetoram and chlorfenapyr in a weight ratio of 80: 1-1: 80. The two active components in the composition have large difference in action mechanism, have obvious synergistic effect on pests such as mosquitoes, hyphantria cunea, locusts, red imported fire ants and the like after being compounded, and have high production and application values.

Description

Insecticidal composition containing methoprene compound and application thereof

Technical Field

The invention relates to an insecticidal composition, in particular to an insecticidal composition containing methoprene compounds and application thereof, and particularly relates to an insecticidal composition used for preventing and controlling biting insects, sucking insects and chewing insects.

Background

The invention belongs to the technical field of pest control, and also belongs to the technical field of sting/sucking insects and chewing insects control. The active compositions of the present invention are useful for controlling biting/sucking insects and chewing insects.

CN201510874155.2 relates to a long-acting, low-toxicity, highly effective insecticidal composition comprising methoprene and at least one nicotinic insecticide for controlling termites, hygiene, storage or crop pests, however, the insecticidal effect of the composition is general.

CN201510881450.0 relates to a safe, environment-friendly and highly effective insecticidal composition and its use, the invention comprises methoprene and at least one pyrethroid insecticide for controlling agricultural pests, controlling sanitary pests, treating animal parasitic infection and killing storage pests, however, in production practice, the control effect and the lasting effect of the composition are general.

CN201210095190.0 relates to an ultra-low volume liquid containing metaflumizone and insect growth regulator insecticide, wherein the insect growth regulator is selected from chlorfluazuron, hexaflumuron, chlorbenzuron, diflubenzuron, buprofezin, fenoxycarb, tebufenozide and the like, and the ultra-low volume liquid is used for preventing and controlling crop pests such as rice planthopper, chilo suppressalis, peach fruit borer, thrips and the like.

Methoprene (Cas: 40596-69-8) and (S) -Methoprene (S-Methoprene, Cas:65733-16-6) belong to juvenile hormone analogues (IRAC 7A), inhibit the growth and development of pests, interfere the desquamation of early-aged larvae, inhibit the larvae from becoming adults, cause malformed pupae or adults, and cause the death or the anogenital capacity of the adults. The methoprene has high activity to many pests of lepidoptera, coleoptera, diptera, hymenoptera and the like, such as fall webworms, pine caterpillars, flies (larvae), cockroaches, fleas, red imported fire ants and the like. The methoprene and the (S) -methoprene have poor activity on adult pests, slow drug effect and high ovicidal activity. Methoprene and (S) -methoprene, which are insect growth regulators, have low mammalian toxicity and low environmental impact, have acquired EPA exemption from food residue requirements in the United states.

Indoxacarb (Indoxacarb, Cas:114171-61-9), Metaflumizone (Metaflumizone, Cas:139968-49-3), Chlorfenapyr (Chlorfenapyr, Cas:122453-73-0), Spinosad (Cas: 168316-95-8) and Spinetoram (Cas: 187166-40-1) act on nerve/muscle/breath, and have the advantages of quick drug effect, poor ovicidal activity and easy generation of resistance after long-term use; the active compound has low toxicity, and can be applied to the non-agricultural fields of sanitation, forestry and the like for preventing and treating pests. The prior art does not disclose the combination of methoprene with these insecticides and the applicants have surprisingly found that these two classes of insecticidal active ingredients when combined have excellent synergistic effects.

Disclosure of Invention

The invention provides an efficient and environment-friendly insecticidal composition for preventing and controlling biting/sucking insects and chewing insects.

The invention also aims to provide a preparation formula and application of the composition.

The above object of the present invention is achieved by the following technical solutions:

an insecticidal composition characterized by: the composition comprises an active component A and an active component B, wherein the active component A is selected from one of (S) -methoprene and methoprene, and the active component B is selected from one of indoxacarb, metaflumizone, spinosad, spinetoram and chlorfenapyr in a weight ratio of 80: 1-1: 80.

Preferably, the weight ratio of the two is 20: 1-1: 20; further preferably 10:1 to 1: 10; still more preferably 5:1 to 1: 5.

The invention further provides an insecticide preparation, which is characterized by comprising the insecticide composition and a suitable pesticide auxiliary agent, wherein the insecticide composition accounts for 0.01-90 wt% of the preparation. Preferably 0.1 to 80 wt%.

The insecticidal composition is prepared into various formulations according to a conventional method, including but not limited to suspending agents, missible oil, aqueous emulsion, micro-emulsion, oil suspending agents, microcapsule suspension-suspending agents, soluble solutions, wettable powder, water dispersible granules, baits, powder and aerosol. The appropriate dosage form is selected according to actual conditions.

The auxiliary components can be wetting agent, dispersant, emulsifier, disintegrant, antifreezing agent, attractant, preservative, defoaming agent, thickening agent, acid-base regulator, binder, solvent, granule carrier, bait, filler, etc.

The wetting agent includes, but is not limited to, one or more of fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, alkyl sulfate, naphthalene sulfonate, alkyl succinate sulfonate and alkylphenol polyoxyethylene ether formaldehyde condensate sulfonate;

the dispersant includes but IS not limited to one or more of polyether phosphate and its salt, alkylphenol polyoxyethylene ether formaldehyde condensate sulfonate, polycarboxylate, block polyether, phenethylphenol polyoxyethylene ether phosphate triethanolamine salt, naphthalene sulfonate formaldehyde condensate, lignosulfonate, nekal, NNO, MF and IS dispersing;

the emulsifier includes but is not limited to one or more of polyether phosphate, T-60, calcium dodecylbenzene sulfonate, ethylene oxide propylene oxide block copolymer, fatty alcohol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene ether, calcium alkyl benzene sulfonate and block polyether;

the disintegrant includes, but is not limited to, one or more of ammonium sulfate, sodium sulfate, glucose, corn starch, ammonium chloride, bentonite, urea, and sodium bicarbonate;

the antifreeze agent comprises but is not limited to one or more of ethylene glycol, propylene glycol, glycerol, sodium chloride, urea, cellosolve, sorbitol, polyvinylpyrrolidone and calcium chloride;

the attractant comprises but is not limited to animal and vegetable oil, lard, soybean oil, peanut oil, rapeseed oil, sesame oil, corn oil, cottonseed oil and wheat germ oil; saccharides, glucose, sucrose, fructose, brown sugar, and honey;

the preservative includes but is not limited to one or more of sodium benzoate, potassium sorbate, sodium dehydroacetate, sodium diacetate, cason, paraben or their sodium salts, bronopol, pentanediol and sodium salicylate;

the antifoaming agent includes, but is not limited to, one or more of silicone compounds, lower alcohols, silicone oils, phosphate esters, and fatty acids and fatty acid esters;

the thickening agent includes, but is not limited to, one or more of xanthan gum, fumed SiO2, bentonite, aluminum stearate, xanthan gum powder, polyvinyl alcohol, modified cellulose, magnesium aluminum silicate, gum arabic, gum, gelatin, guar gum, alginic acid, agar, and tall oil;

the pH modifier includes, but is not limited to, one or more of citric acid, oxalic acid, ammonium chloride, tartaric acid and succinic acid;

the solvent includes but is not limited to one or more of C6-C30 isoparaffin or dearomatization solvent oil, methyl laurate, diethyl maleate, tributyl phosphate and other low-toxicity tasteless monoesters, diester or triester solvents, diisooctyl phthalate, trans-2-hexenol, diesel oil, diisopropyl diacid, dimethyl phthalate, vegetable oil, engine oil and dimethyl sulfoxide;

the granule carrier comprises but is not limited to one or more of attapulgite particles, volcanic rock particles, red brick particles, coal gangue particles, medical stone particles, zeolite particles, corncob particles and walnut shell particles with better adsorption performance;

the bait comprises but is not limited to one or more of wheat flour, soybean flour, corn flour, potato flour, biscuit flour, grain flour and peanut flour;

the filler includes but is not limited to one or more of kaolin, white carbon black, china clay, bentonite, attapulgite, diatomite, white carbon black and light calcium carbonate;

the preparation prepared from the composition is used directly or after being diluted by a solvent, and the application method is selected according to the properties of the composition according to the purpose to be achieved and the environment, and the pests or the habitats thereof are treated by spraying, ultralow volume, retention spraying, hot smoke, spraying, powder spraying, broadcasting, feeding, watering and the like.

The present invention relates to insecticidal compositions suitable for controlling arthropods, more preferably biting and sucking insects, chewing insects and arachnids acarids.

The biting and sucking insects include, but are not limited to, mosquitoes (e.g., aedes aegypti, aedes albopictus, aedes assailing, culex fatigues, culex pallens, culex haranthis, culex pipiens, culex murraya, anopheles gambiae, anopheles arabica, anopheles nigra, anopheles minutiae, anopheles macrolepsis, anopheles schoenopsis sinensis, anopheles hygrophila, etc.); flies (houseflies, summer toilet flies, tsetse flies, cattle flies, maggot-causing flies, cattle flies, blowflies, dermatoglyphus, gastrointestinal flies, trypanosoma mansoni, etc.); biting midges (e.g., Culicoides), gnats (e.g., gnat), sand flies (e.g., sand flies); fleas (e.g., manfleas, xenorhabdus, ctenocephalides canis, ctenocephalides felis, etc.); lice (e.g., pediculus humanus, lice of swine, lice of sheep and livestock, etc.); bed bugs (e.g., cimex fusca, rhynchophorus ferrugineus, etc.).

Preferably, the biting and sucking insects are: mosquitoes, fleas and bed bugs.

The chewing insects include but are not limited to cockroaches (such as german cockroach, periplaneta americana, australia cockroach, brown cockroach, black chest cockroach, etc.); ants (such as black ant, little yellow ant, etc.); solenopsis invicta, termite; grassland pest locusts (locusts migratoria of east asia, locusts migratoria of tibetan, etc.); forestry pests (lepidoptera larvae), fall moths, pine caterpillars (e.g., pine caterpillar, larch caterpillar, masson pine caterpillar, etc.), poisonous moths (e.g., gypsy moth, willow moth, etc.), boat moths (e.g., poplars canula moth, poplarch boat moth, etc.), chi moths (e.g., wood inchworm, large bridgeworm, pear chi moth, etc.); beetles (such as grain elephant, yellow meal worm, hamamelis, medicinal material A, furniture larch, domestic longicorn, etc.); moth larvae (such as tobacco meal moth, Indian corn borer, bagel corn moth, and felty moth).

Preferably, the chewing insects are: red imported fire ant, fall webworm and boat moth.

Compared with the prior art, the invention has the beneficial effects that:

(1) the insect growth regulator is compounded with the insecticide acting on nerve/muscle/breath, has complementary advantages, can effectively control the insect state of each stage of the whole life cycle of pests, reduces the using amount of the insecticide and delays the drug resistance.

(2) The active components of the invention have large difference of action mechanism, and have obvious synergistic effect on pests such as mosquitoes, fall webworms, locusts, red imported fire ants and the like after being compounded.

(3) The insecticidal composition of the invention has low toxicity, high efficiency and environmental protection, and is suitable for preventing and treating pests in the non-agricultural fields of sanitation, forestry, grassland and the like.

Detailed Description

The present invention is further illustrated by the following examples, which are not intended to limit the invention to these embodiments. It will be appreciated by those skilled in the art that the present invention encompasses all alternatives, modifications and equivalents as may be included within the scope of the claims.

Bait example 1: (S) -methoprene bait (0.1%)

The bait agent of the embodiment takes the methoprene as an active ingredient, the soybean meal as bait, a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) as a solenopsis invicta attractant, and the potassium sorbate as a preservative, wherein the active ingredient (S) -methoprene accounts for 0.1 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent by weight.

Bait example 2: metaflumizone bait (0.1%)

The bait agent takes metaflumizone as an active ingredient, soybean meal as bait, a mixture (the mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as a solenopsis invicta attractant, and potassium sorbate as a preservative, wherein the metaflumizone accounts for 0.1 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent by weight.

Bait example 3: (S) -methoprene-metaflumizone bait (0.1% + 0.09%)

In the bait agent, the (S) -methoprene and the metaflumizone are used as active ingredients, the soybean meal is used as bait, a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) is used as an attractant for the solenopsis invicta, and the potassium sorbate is used as a preservative, wherein the active ingredients comprise, by weight, 0.09% of the (S) -methoprene, 0.01% of the metaflumizone, 64.4% of the bait soybean meal, 35% of the attractant mixture and 0.5% of the potassium sorbate as a preservative.

Bait example 4: (S) -methoprene-metaflumizone bait (0.1% + 0.08%)

In the bait agent, the (S) -methoprene and the metaflumizone are used as active ingredients, the soybean meal is used as bait, a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) is used as an attractant for the solenopsis invicta, and the potassium sorbate is used as a preservative, wherein the active ingredients comprise, by weight, 0.08% of the (S) -methoprene, 0.02% of the metaflumizone, 64.4% of the bait soybean meal, 35% of the attractant mixture and 0.5% of the potassium sorbate as a preservative.

Bait example 5: (S) -methoprene-metaflumizone bait (0.1% + 0.05%)

In the bait agent, the (S) -methoprene and the metaflumizone are used as active ingredients, the soybean meal is used as bait, a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) is used as an attractant for the solenopsis invicta, and the potassium sorbate is used as a preservative, wherein the active ingredients comprise, by weight, 0.05% of the (S) -methoprene, 0.05% of the metaflumizone, 64.4% of the bait soybean meal, 35% of the attractant mixture and 0.5% of the potassium sorbate as a preservative.

Bait example 6: (S) -methoprene-metaflumizone bait (0.1% + 0.02%)

In the bait agent, the (S) -methoprene and the metaflumizone are used as active ingredients, the soybean meal is used as bait, a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) is used as an attractant for the solenopsis invicta, and the potassium sorbate is used as a preservative, wherein the active ingredients comprise, by weight, 0.02% of the (S) -methoprene, 0.08% of the metaflumizone, 64.4% of the bait soybean meal, 35% of the attractant mixture and 0.5% of the potassium sorbate as a preservative.

Bait example 7: (S) -methoprene-metaflumizone bait (0.1% + 0.01%)

In the bait agent, the (S) -methoprene and the metaflumizone are used as active ingredients, the soybean meal is used as bait, a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) is used as an attractant for the solenopsis invicta, and the potassium sorbate is used as a preservative, wherein the active ingredients comprise, by weight, 0.01% of the (S) -methoprene, 0.09% of the metaflumizone, 64.4% of the bait soybean meal, 35% of the attractant mixture and 0.5% of the potassium sorbate as a preservative.

Bait example 8: spinetoram bait (0.1%)

The bait agent of the embodiment takes the spinetoram as an active ingredient, adopts the soybean meal as bait, adopts a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) as a solenopsis invicta attractant, and adopts the potassium sorbate as a preservative, wherein the spinetoram bait agent accounts for 0.1 percent, the soybean meal of the bait accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the potassium sorbate as a preservative accounts for 0.5 percent in percentage by weight.

Bait example 9: (S) -methoprene-spinetoram bait (0.1% + 0.095%)

The bait agent of the embodiment takes the methoprene and the spinetoram as active ingredients, the soybean meal as bait, a mixture (mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as an attractant for solenopsis invicta, and the potassium sorbate as a preservative, wherein the active ingredients (S) -methoprene account for 0.095 percent, the spinetoram accounts for 0.005 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent by weight.

Bait example 10: (S) -methoprene-spinetoram bait (0.1% + 0.0875% + 0.0125%)

The bait agent of the embodiment takes the methoprene and the spinetoram as active ingredients, adopts the soybean meal as bait, adopts a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) as a solenopsis invicta attractant, and adopts the potassium sorbate as a preservative, wherein the active ingredient (S) -methoprene accounts for 0.0875 percent, the spinetoram accounts for 0.0125 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent by weight.

Bait example 11: (S) -methoprene-spinetoram bait (0.1% + 0.05%)

The bait agent of the embodiment takes the methoprene and the spinetoram as active ingredients, adopts the soybean meal as bait, adopts a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) as a solenopsis invicta attractant, and adopts the potassium sorbate as a preservative, wherein the active ingredient (S) -methoprene accounts for 0.05 percent, the spinetoram accounts for 0.05 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent by weight.

Bait example 12: (S) -methoprene-spinetoram bait (0.1% + 0.025%)

The bait agent of the embodiment takes the methoprene and the spinetoram as active ingredients, the soybean meal as bait, a mixture (mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as an attractant for the solenopsis invicta, and the potassium sorbate as a preservative, wherein the active ingredient (S) -methoprene accounts for 0.025 percent, the spinetoram accounts for 0.075 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent by weight.

Bait example 13: (S) -methoprene-spinetoram bait (0.1% + 0.01%)

The bait agent of the embodiment takes the methoprene and the spinetoram as active ingredients, adopts the soybean meal as bait, adopts a mixture of honey, peanut oil and cane sugar (the mixing ratio is 1:0.2:2) as a solenopsis invicta attractant, and adopts the potassium sorbate as a preservative, wherein the active ingredient (S) -methoprene accounts for 0.01 percent, the spinetoram accounts for 0.09 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent by weight.

Bait example 14: methoprene bait (0.1%)

The bait agent of the embodiment takes the methoprene as an active ingredient, the soybean meal as bait, a mixture (mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as an attractant for the solenopsis invicta, and the potassium sorbate as a preservative, wherein the active ingredient comprises 0.1% of the methoprene, 64.4% of the bait soybean meal, 35% of the attractant mixture and 0.5% of the preservative potassium sorbate in percentage by weight.

Bait example 15: indoxacarb bait (0.1%)

The bait agent of the embodiment takes the indoxacarb as an active ingredient, the soybean meal as bait, a mixture (the mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as a solenopsis invicta attractant, and potassium sorbate as a preservative, wherein the indoxacarb accounts for 0.1 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative potassium sorbate accounts for 0.5 percent in percentage by weight.

Bait example 16: bait of methoprene and indoxacarb (0.1% + 0.08% + 0.02%)

The bait agent of the embodiment takes the methoprene and the indoxacarb as active ingredients, the soybean meal as bait, a mixture (mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as an attractant for solenopsis invicta, and the potassium sorbate as a preservative, wherein the active ingredients comprise, by weight, 0.08% of the methoprene, 0.02% of the indoxacarb, 64.4% of the bait soybean meal, 35% of the attractant mixture, and 0.5% of the preservative potassium sorbate.

Bait example 17: bait of methoprene and indoxacarb (0.1% + 0.02% + 0.08%)

The bait agent of the embodiment takes the methoprene and the indoxacarb as active ingredients, the soybean meal as bait, a mixture (mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as an attractant for solenopsis invicta, and the potassium sorbate as a preservative, wherein the active ingredients comprise, by weight, the methoprene 0.02%, the indoxacarb 0.08%, the bait soybean meal 64.4%, the attractant mixture 35% and the preservative potassium sorbate 0.5%.

Bait example 18: chlorfenapyr bait (0.1%)

The bait agent of the embodiment takes the chlorfenapyr as an active ingredient, the soybean meal as bait, a mixture (the mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as a solenopsis invicta attractant, and the potassium sorbate as a preservative, wherein the active ingredient, namely the chlorfenapyr accounts for 0.1 percent, the bait soybean meal accounts for 64.4 percent, the attractant mixture accounts for 35 percent, and the preservative, namely the potassium sorbate accounts for 0.5 percent in percentage by weight.

Bait example 19: bait of methoprene and chlorfenapyr (0.1% + 0.075% + 0.025%)

The bait agent of the embodiment takes the methoprene and the chlorfenapyr as active ingredients, the soybean meal as bait, a mixture (mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as an attractant for the solenopsis invicta, and the potassium sorbate as a preservative, wherein the active ingredients comprise 0.075 percent of the methoprene, 0.025 percent of the indoxacarb, 64.4 percent of the soybean meal bait, 35 percent of the attractant mixture and 0.5 percent of the preservative potassium sorbate in percentage by weight.

Bait example 20: bait of methoprene and chlorfenapyr (0.1% ═ 0.025% + 0.075%)

The bait agent of the embodiment takes the methoprene and the chlorfenapyr as active ingredients, the soybean meal as bait, a mixture (mixing ratio is 1:0.2:2) of honey, peanut oil and cane sugar as an attractant for the solenopsis invicta, and the potassium sorbate as a preservative, wherein the active ingredients comprise 0.025 percent of the methoprene, 0.075 percent of the indoxacarb, 64.4 percent of the soybean meal bait, 35 percent of the attractant mixture and 0.5 percent of the preservative potassium sorbate in percentage by weight.

Application example 1: laboratory test of culex pipiens pallens larvae

Test subjects: culex pipiens pallens, larvae of 3 years old, indoor sensitive strain.

Test agents: respectively weighing (S) -methoprene, pleocidin and indoxacarb raw medicines, adding a proper amount of acetone for dissolving, adding distilled water to prepare mother liquor with required concentration, and mixing the mother liquor according to a certain proportion to prepare mixed mother liquor.

The test method comprises the following steps: reference pesticide registration sanitary insecticide indoor efficacy test and evaluation part 5: the mosquito larva control agent NY/T1151.5-2014 is prepared by taking a white porcelain basin with the diameter of 30cm and the depth of 10cm, putting clear water with the depth of 8cm into the basin, putting 50 larvae of healthy and pale culex 3 years old into each basin, adding the prepared agent according to the dosage according to the water area in the basin, observing the death and pupation conditions of the larvae every day, and taking the pupae out to observe the eclosion condition of the larvae. Three replicates were used and a blank control was set.

And (3) test results: in the table 1, the (S) -methoprene is compounded with the spinosad/indoxacarb, and by utilizing different action mechanisms, the composition has killing effects on both larvae and pupae of culex pipiens pallens, and the prevention and treatment rate of the compounded preparation is higher than that of a single preparation under the same dosage.

TABLE 1(S) -methoprene compounded mixture killing effect on culex pipiens pallens larvae and pupae

Application example 2: laboratory test of Aedes albopictus larvae

Test subjects: aedes albopictus, 3-instar larvae, indoor sensitive strain.

Test agents: weighing raw medicines of methoprene, spinetoram, metaflumizone and chlorfenapyr respectively, adding a proper amount of acetone for dissolving, adding distilled water for preparing mother liquor with required concentration, and mixing the mother liquor according to a certain proportion to prepare mixed mother liquor.

The test method comprises the following steps: reference pesticide registration sanitary insecticide indoor efficacy test and evaluation part 5: the mosquito larva control agent NY/T1151.5-2014 is prepared by taking a white porcelain basin with the diameter of 30cm and the depth of 10cm, putting clear water with the depth of 8cm into the basin, putting 50 healthy aedes albopictus larvae into each basin, adding the prepared agent according to the dosage according to the water area in the basin, observing the death and pupation conditions of the larvae every day, and taking out the pupae to observe the eclosion condition of the larvae. Three replicates were used and a blank control was set.

And (3) test results: as shown in Table 2, the combination of the methoprene and the spinetoram/the metaflumizone/the chlorfenapyr has killing effects on larvae and pupae of aedes albopictus by utilizing different action mechanisms, and the prevention and treatment rate of the compound preparation is higher than that of a single agent under the same dosage.

TABLE 2 killing effect of the methoprene compound mixture on larvae and pupae of Aedes albopictus

Application example 3: bed bug laboratory test

Test subjects: temperature bed bugs, eggs.

Reagent to be tested: respectively dissolving (S) -methoprene, pleocidin, metaflumizone and chlorfenapyr with absolute ethyl alcohol, and diluting with water to prepare the required mother liquor.

The test method comprises the following steps: preparing the mother liquor into a series of concentrations by adopting an immersion method; transferring the eggs to a beaker with liquid medicine, treating the eggs with the liquid medicine, and transferring the eggs to a normal condition for culture; repeating for 3 times, and setting clear water control, wherein each cup contains 20 eggs; and (5) surveying and recording the number of hatched eggs and the number of dead eggs, and calculating the egg death rate.

The evaluation method comprises the following steps: the method is one of the classical methods for evaluating the effect of the mixture by adopting a Colby formula. The Colby formula is as follows: e ═ X + Y- (X × Y)/100,

wherein X is the mortality in m ppm of active compound A, expressed as a percentage of the untreated control; y is the mortality rate at a concentration of n ppm of active compound B, expressed as a percentage of the untreated control group, and E is the mortality rate when active compounds A and B are applied at an application rate of m + nppm, expressed as a percentage of the untreated control group.

If the actual mortality rate of the target after the two active ingredients are mixed at a certain concentration is greater than the theoretical mortality rate E, the two active ingredients are judged to have synergistic effect when being mixed at a set concentration, otherwise, the two active ingredients are antagonistic.

And (3) test results: see table 3, (S) -methoprene/methoprene compounded with spinosad/metaflumizone/chlorfenapyr has a synergistic effect on temperature bug eggs.

TABLE 3 killing effect of (S) -methoprene/methoprene compound mixture on temperature stink eggs

Application example 4: indoor test for fleas

Test subjects: cyperus bigelovii, ovum.

Reagent to be tested: dissolving (S) -methoprene, indoxacarb and ethidium pesticide with absolute ethyl alcohol respectively, and diluting with water to prepare the required mother liquor.

The evaluation method comprises the following steps: the method is one of the classical methods for evaluating the effect of the mixture by adopting a Colby formula.

And (3) test results: see table 4, the combination of (S) -methoprene/methoprene with indoxacarb/spinetoram has a synergistic effect on eggs of paddlefish.

TABLE 4 killing effect of (S) -methoprene/methoprene compound mixture on eggs of Trichoplusia bigelovii

Application example 5: laboratory test of fall webworm

Test subjects: fall webworm, 3 instar larva.

Reagent to be tested: respectively dissolving (S) -methoprene, spinosad, metaflumizone and chlorfenapyr with absolute ethyl alcohol, and preparing the required mother liquor by using an aqueous solution containing 0.1% of tween 80.

The test method comprises the following steps: preparing the mother liquor into a series of concentrations by adopting an immersion method; soaking fresh poplar leaves which are not polluted by the pesticide in the liquid medicine to be tested for 5s, taking out the poplar leaves, drying the poplar leaves in the shade, wrapping petioles with absorbent cotton, soaking the cotton leaves in clear water, putting the cotton leaves into a plastic cup, testing insects for 15 times in each cup, repeating the steps for 3 times, and establishing clear water contrast; the eggs are placed under the conditions of humidity of 75%, temperature of 24 ℃ and illumination of 14:10(L: D) for feeding, and the number of dead insects and the total number of insects are investigated and recorded after 72 hours.

Performing statistical analysis by POLO statistical analysis software to calculate LC of each medicine₅₀And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.

Measured toxicity index (ATI) ═ standard medicament LC₅₀Test agent LC₅₀)×100。

Theoretical virulence index (TTI) ═ a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture.

Co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].

According to NY/T1154.7-2006 pesticide combination division standard: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80 < co-toxicity coefficient (CTC) < 120 showed additive effects.

And (3) test results: in the table 5, the synergistic effect is shown when the ratio of (S) -methoprene to spinosad is 20: 1-1: 80, and the co-toxicity coefficient is the highest and exceeds 184 when the ratio is 5:1-1: 5; when the ratio of the (S) -methoprene to the metaflumizone is 10:1-1:10, the co-toxicity coefficients are all more than 120, and the synergistic effect is shown, and when the ratio is 5:1-1:5, the co-toxicity coefficients are higher than 168-191; when the ratio of the (S) -methoprene to the chlorfenapyr is 20: 1-1: 20, the co-toxicity coefficient is more than 120, the synergistic effect is shown, and when the ratio is 5:1-1:5, the co-toxicity coefficient is higher than 147-172.

TABLE 5 indoor joint virulence determination of (S) -methoprene compounded mixture against fall webworm

Application example 6: poplar and small boat moth indoor test

Test subjects: poplar canna, 3 instar larva.

Reagent to be tested: respectively dissolving the raw pesticide methoprene, spinetoram and indoxacarb with absolute ethyl alcohol, and preparing the required mother liquor by using an aqueous solution containing 0.1% of tween 80.

And (3) test results: as shown in Table 6, when the ratio of the methoprene to the spinetoram is 20: 1-1: 80, the co-toxicity coefficients are all more than 120, and the synergistic effect is shown, and when the ratio is 5:1-1:5, the co-toxicity coefficients are higher to 173-191; when the ratio of the methoprene to the indoxacarb is 20: 1-1: 20, the co-toxicity coefficient is more than 120, and the synergistic effect is shown, and when the ratio is 5:1-1:5, the co-toxicity coefficient is higher to 194-219.

TABLE 6 indoor joint toxicity determination of methoprene compounded mixture to Populus makinoi

Application example 7: in situ solenopsis invicta control test

Test field: quanzhou Jinjiang inner hole town.

The control object is: and (4) red imported fire ants.

Test agents: bait formulations examples 1-20, blank bait was used as a control formulation.

The test method comprises the following steps: reference pesticide field efficacy test criteria (second) section 149: the pesticide is used for preventing and controlling red imported fire ants GB/T7980.149-2009: the test had 20 treatments (bait examples 1-20) with blank bait as a control agent, repeated 3 times, each treatment preceded by a 3-5 meter isolation zone; the ground surface temperature is 20-35 ℃, the pesticide is applied when the ground surface is dry, the pesticide is annularly spread in a range of 30-100 cm away from the ant nest, the dosage of the preparation is 20 g/nest, and the preparation needs to be applied when raining within 2 days after the pesticide is applied; investigating the density of 4 workers before application, 7 days, 30 days and 45 days after application (adopting fresh ham sausage piece trapping method); and (3) calculating the prevention effect of the worker ants, wherein the calculation formula is as follows:

the termite control effect (%) is (1-average termite number after drug trapping/average termite number after drug trapping) x 100.

And (3) test results: in table 7, under the same control dosage, the (S) -methoprene/methoprene compound solenopsis invicta bait has a higher control rate and a longer effective period than a single dose.

TABLE 7(S) -methoprene/methoprene composite bait for controlling solenopsis invicta on site

The insecticidal composition containing methoprene and the application thereof have been described by specific examples, and other objects can be achieved by appropriate changes of raw materials and process conditions without departing from the invention, which is obvious for those skilled in the art and is considered to be included in the scope of the invention.

Claims

1. An insecticidal composition characterized by: the composition comprises an active component A and an active component B, wherein the active component A is selected from one of (S) -methoprene and methoprene, and the active component B is selected from one of indoxacarb, metaflumizone, spinosad, spinetoram and chlorfenapyr in a weight ratio of 80: 1-1: 80.

2. The insecticidal composition of claim 1, wherein: the weight ratio of the two is 20: 1-1: 20.

3. The insecticidal composition of claim 1, wherein: the weight ratio of the two is 5:1-1: 5.

4. An insecticidal formulation comprising an insecticidal composition according to any one of claims 1 to 3 and a pesticidally acceptable adjuvant, wherein the insecticidal composition comprises from 0.01 to 90% by weight of the formulation.

5. Use of an insecticidal composition according to any one of claims 1 to 3 or claim 4 for controlling biting and sucking insects, chewing insects and arachnid acarids.

6. The use of claim 7, said biting and sucking insects being: mosquitoes, fleas or bed bugs.

7. The use of claim 7, the chewing insect being: red imported fire ants, fall webworm or boat moth.