CN116349576A - Seedling substrate - Google Patents
Seedling substrate Download PDFInfo
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- CN116349576A CN116349576A CN202310366799.5A CN202310366799A CN116349576A CN 116349576 A CN116349576 A CN 116349576A CN 202310366799 A CN202310366799 A CN 202310366799A CN 116349576 A CN116349576 A CN 116349576A
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- 239000000758 substrate Substances 0.000 title claims abstract description 34
- 239000005783 Fluopyram Substances 0.000 claims abstract description 27
- KVDJTXBXMWJJEF-UHFFFAOYSA-N fluopyram Chemical compound ClC1=CC(C(F)(F)F)=CN=C1CCNC(=O)C1=CC=CC=C1C(F)(F)F KVDJTXBXMWJJEF-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000002156 mixing Methods 0.000 claims abstract description 19
- 239000003337 fertilizer Substances 0.000 claims abstract description 16
- 239000011159 matrix material Substances 0.000 claims abstract description 15
- 239000002689 soil Substances 0.000 claims abstract description 13
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 12
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 8
- 239000003415 peat Substances 0.000 claims abstract description 7
- 239000010451 perlite Substances 0.000 claims abstract description 7
- 235000019362 perlite Nutrition 0.000 claims abstract description 7
- 239000010455 vermiculite Substances 0.000 claims abstract description 7
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 7
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 7
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 6
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 6
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910052939 potassium sulfate Inorganic materials 0.000 claims abstract description 6
- 235000011151 potassium sulphates Nutrition 0.000 claims abstract description 6
- 239000003124 biologic agent Substances 0.000 claims abstract description 4
- 239000003814 drug Substances 0.000 claims description 14
- 241000223924 Eimeria Species 0.000 claims description 8
- 229940079593 drug Drugs 0.000 claims description 7
- 241000193764 Brevibacillus brevis Species 0.000 claims description 5
- 239000000375 suspending agent Substances 0.000 claims description 4
- 241000243786 Meloidogyne incognita Species 0.000 abstract description 20
- 230000012010 growth Effects 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 241000244206 Nematoda Species 0.000 description 14
- 238000012360 testing method Methods 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 240000008067 Cucumis sativus Species 0.000 description 4
- 241000243785 Meloidogyne javanica Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 235000009849 Cucumis sativus Nutrition 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 235000001231 Streptopus amplexifolius Nutrition 0.000 description 3
- 244000305550 Streptopus amplexifolius Species 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- XLROVYAPLOFLNU-UHFFFAOYSA-N protactinium atom Chemical compound [Pa] XLROVYAPLOFLNU-UHFFFAOYSA-N 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 description 1
- 239000005660 Abamectin Substances 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 239000005959 Fosthiazate Substances 0.000 description 1
- 241001143352 Meloidogyne Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 229950008167 abamectin Drugs 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 235000020299 breve Nutrition 0.000 description 1
- JLQUFIHWVLZVTJ-UHFFFAOYSA-N carbosulfan Chemical compound CCCCN(CCCC)SN(C)C(=O)OC1=CC=CC2=C1OC(C)(C)C2 JLQUFIHWVLZVTJ-UHFFFAOYSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- CXEGAUYXQAKHKJ-NSBHKLITSA-N emamectin B1a Chemical compound C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](NC)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 CXEGAUYXQAKHKJ-NSBHKLITSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- DUFVKSUJRWYZQP-UHFFFAOYSA-N fosthiazate Chemical compound CCC(C)SP(=O)(OCC)N1CCSC1=O DUFVKSUJRWYZQP-UHFFFAOYSA-N 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of seedling raising substrates, and particularly relates to a seedling raising substrate. The seedling substrate is prepared by mixing the following components in percentage by mass: 90-95% of matrix component, 0.5-0.75% of biological microbial inoculum component and the balance of fertilizer component; wherein, the matrix components are composed of vermiculite, perlite, peat soil and coconut coir according to the weight ratio of 4:1:1:2, mixing the materials according to the mass ratio; the biological agent is prepared by mixing Equilibrium brevifolium and fluopyram; the fertilizer comprises the following components of potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate according to the proportion of 8:4:3 by mass ratio. The seedling substrate provided by the invention consists of three parts, namely a substrate component, a biological microbial inoculum and a fertilizer component, wherein the substrate component can provide an excellent growth environment for crop seedlings, the fertilizer component can provide nutrients for the crop seedlings, and the biological microbial inoculum can effectively defend the influence of the meloidogyne incognita on the crop seedlings, so that the survival and healthy growth of the crop seedlings can be ensured.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a seedling substrate.
Background
The seedling substrate can be used for seedling raising of crops such as cucumbers, but the seedling raising of the crops such as conventional cucumbers is easily damaged by the meloidogyne incognita. The southern root-knot nematode damages the plant root system structure mainly through invading wounds and forming tumor-shaped root knots, influences the normal metabolic activity of plants, and causes the coincidence of infection of other pathogenic bacteria, so that the plant growth vigor is weakened or even dead, and the yield of crops is reduced or even is dead.
However, many seedling substrates only provide nutrients and normal environment for crop seedlings, and do not have the hazard of preventing meloidogyne incognita. If a proper amount of pesticide components for killing the meloidogyne incognita are added into the seedling raising matrix, the meloidogyne incognita can be prevented and controlled to a certain extent, the survival and normal growth of crop seedlings such as cucumbers are ensured, and the smooth development of seedling raising work is facilitated. In the prior art, the medicines for preventing and controlling the meloidogyne incognita are multiple, such as abamectin, emamectin benzoate, fosthiazate, carbosulfan and the like, but the medicine effect of a single medicine is limited under the conventional dosage due to the accumulation of the medicine resistance of the meloidogyne incognita, so that the use of a compound preparation or a mixed preparation for preventing and controlling the meloidogyne incognita is necessary. At present, no report is known about the compounding of the addition of the Eimeria brevis and the fluopyram in the seedling culture matrix.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a seedling substrate which can effectively defend the influence of meloidogyne incognita on crop seedlings, and further can ensure the normal growth of the crop seedlings.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the seedling substrate is prepared by mixing the following components in percentage by mass: 90-95% of matrix component, 0.5-0.75% of biological microbial inoculum component and the balance of fertilizer component; wherein, the matrix components are composed of vermiculite, perlite, peat soil and coconut coir according to the weight ratio of 4:1:1:2, mixing the materials according to the mass ratio;
the biological agent is prepared by mixing Equilibrium brevifolium and fluopyram;
the fertilizer comprises the following components of potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate according to the proportion of 8:4:3 by mass ratio.
Preferably, the Emamacter brevis is 300 hundred megaspores/gram of Emamacter brevis parent drug; the fluopyram suspension agent comprises 41.7% of fluopyram by mass fraction.
Preferably, the mass ratio of the Eimeria brevis to the fluopyram is 1-7:19-1.
Preferably, the mass ratio of the Eimeria brevis to the fluopyram is 1:7.
compared with the prior art, the invention has the following beneficial effects:
(1) The seedling substrate provided by the invention consists of three parts, namely a substrate component, a biological microbial inoculum and a fertilizer component, wherein the substrate component can provide an excellent growth environment for crop seedlings, the fertilizer component can provide nutrients for the crop seedlings, and the biological microbial inoculum can effectively defend the influence of the meloidogyne incognita on the crop seedlings, so that the survival and healthy growth of the crop seedlings can be ensured.
(2) The biological microbial inoculum is prepared by mixing the Equilibrium brevium and fluopyram, and the biological microbial inoculum has synergistic effect on the southern root-knot nematodes in a certain mass ratio range during the mixing of the Equilibrium brevium and fluopyram, so that the control effect on the southern root-knot nematodes can be improved.
Detailed Description
The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
In the following examples, vermiculite, perlite, peat soil and coir are commercially available common seedling substrate materials, and have no special requirements.
Example 1: indoor biological activity of mixed of Equilibrium breve and fluopyram
1. Test object: nannon root-knot nematode (Meloidogyne incognita)
2. Test agent
300 hundred million spores/gram of Equilibrium mother medicine (Zhenjiang Runyu Biotechnology development Co., ltd., accession number: PD 20130367)
41.7% fluopyram suspension (Bayer Co., ltd.; accession number: PD 20121664)
Preparing 1000mg/L (mass fraction of the parent drug of the Equilibrium brevicum is 100%) of the effective components of the Equilibrium brevicum and the fluopyram, and then setting a plurality of groups of proportions, wherein each single dose and the proportion mixture are diluted into 5 mass concentration gradients by using a tween-80 aqueous solution with mass fraction of 0.1% for later use.
3. The test method comprises the following steps: (reference to NT/Y1833.1-2009 methods for testing nematode dipping in the pesticide section 1 method for inhibiting plant pathogenic nematodes by means of biological assay in pesticide chambers)
3.1 picking eggs of the root knot nematodes from cucumber roots, cleaning with clear water, placing the eggs on wet filter paper of a culture dish, and incubating at 25 ℃ to obtain second-instar larvae with consistent instar; eluting the cultured root-knot nematode with water, filtering, centrifuging at 1000r/min for 2min, discarding supernatant, adding water, centrifuging again, and finally re-suspending the root-knot nematode with water to 200 head/mL for use.
3.2, sequentially sucking 3mL of liquid medicine from low concentration to high concentration by a pipette, respectively adding the liquid medicine into a test tube, then sucking 3mL of prepared equivalent nematode suspension, and adding the prepared equivalent nematode suspension into the test tube, so that the liquid medicine and the nematode suspension are uniformly mixed in equivalent; transferring a certain volume of the mixed solution into the small holes of a 24-hole biochemical test plate by using a liquid transfer device, covering, and culturing for 24 hours at a constant temperature of 25 ℃; the treatment without the agent was also set as a blank.
3.3 taking 1mL of the mixed solution from each treatment, observing the death condition of the nematodes under an dissecting mirror, recording the number of the bus worms and the number of the dead nematodes for investigation every time the number of the nematodes is repeatedly observed, wherein the judgment standard of the death of the nematodes is that the nematodes are stiff, and the nematodes cannot bend when touched by a hairline needle or a bamboo filament needle. From the survey data, mortality and corrected mortality for each treatment were calculated.
In the above formula: p- -mortality in units of; k- -number of dead insects; n- -total number of insects treated.
In the above formula: p (P) 1 -correct mortality in units of; p (P) t -mortality rate in units of treatment; p (P) 0 Blank mortality in%.
4. Data analysis: regression analysis of the log concentration values of each treatment agent and the corrected mortality probability values of each treatment was performed using DPS software to calculate LC of each treatment agent 50 And co-toxicity coefficient (CTC) was calculated according to grand cloud pei method.
5. Evaluation of drug efficacy
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in Table 1.
Table 1 indoor biological Activity of Eimeria brevis and Fluopyram in combination against Meloidogyne incorporae
As shown in table 1, after the active ingredients of the bacillus brevis and the fluopyram are mixed, the mass ratio is 1: the co-toxicity coefficient of the compound is greater than 120 for the meloidogyne incognita at 19-1, and the compound shows a synergistic effect, especially when the mass ratio is 1: the synergy is most remarkable at 7. In addition, when the mass ratio is 5-7:1, the co-toxicity coefficient of the meloidogyne incognita is more than 80 and less than 120, and the sum is shown as an additive effect.
Example 2
The seedling substrate is prepared by mixing the following components in percentage by mass: 94% of matrix component, 0.5% of biological microbial inoculum component and the balance of fertilizer component; wherein, the matrix components are composed of vermiculite, perlite, peat soil and coconut coir according to the weight ratio of 4:1:1:2, mixing the materials according to the mass ratio;
the biological agent is prepared by mixing Equilibrium brevifolium and fluopyram; the bacillus brevis is 300 hundred million spores/gram of bacillus brevis parent drug, and the fluopyram is 41.7% of fluopyram suspending agent by mass fraction; the mass ratio of the Eimeria brevis to the fluopyram is 1:7, preparing a base material;
the fertilizer comprises the following components of potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate according to the proportion of 8:4:3 by mass ratio.
Comparative example 1
The seedling substrate is prepared by mixing the following components in percentage by mass: 94% of matrix component, 0.5% of Equilibrium brevis and the balance of fertilizer component; wherein, the matrix components are composed of vermiculite, perlite, peat soil and coconut coir according to the weight ratio of 4:1:1:2, mixing the materials according to the mass ratio; the Equilibrium is 300 hundred million spores/gram of Equilibrium brevicum parent drug; the fertilizer comprises the following components of potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate according to the proportion of 8:4:3 by mass ratio.
Comparative example 2
The seedling substrate is prepared by mixing the following components in percentage by mass: 94% of matrix component, 0.5% of fluopyram and the balance of fertilizer component; wherein, the matrix components are composed of vermiculite, perlite, peat soil and coconut coir according to the weight ratio of 4:1:1:2, mixing the materials according to the mass ratio; the fluopyram is a fluopyram suspending agent with the mass fraction of 41.7%; the fertilizer comprises the following components of potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate according to the proportion of 8:4:3 by mass ratio.
Influence of different seedling substrates on cucumber root knot nematode disease
1. Test seedling
Cucumber seedlings of Zhongnong No. 6, 2-3 true leaf stage.
2. Seedling substrate for test
Seedling substrates of example 2, comparative example 1 and comparative example 2.
3. Test method
3.1 grafting the meloidogyne incognita into the seedling substrate to be tested, so that the quantity of the meloidogyne incognita in each 100g seedling substrate is 300-350
3.2 the test seedling substrate was placed in plastic pots (7 cm. Times.7 cm), 200 g/pot, and then the test seedlings were transplanted into the plastic pots, 1 plant per pot. 15 replicates were set for each test seedling substrate.
3.3 after culturing for 30d in a conventional manner, the root knot count was measured and the individual root knot count was calculated.
4. Determination method for meloidogyne incognita in soil
Fully and uniformly mixing soil samples, taking 100g, placing the soil samples in a plastic barrel, adding 1000mL of water, stirring with force to disperse soil blocks, allowing upper liquid to pass through a 160-mesh to 400-mesh sieve sequentially after the layers fall for 1min, continuously flushing with water during the period, collecting residues on the 400-mesh sieve, washing with clear water, fixing the volume to 50mL, sucking 1mL in a culture dish, and observing under a microscope. And calculating the individual number of the line insects in each 100 soil.
5. Test results
The results are shown in Table 2.
TABLE 2 Effect of different seedling substrates on cucumber root knot nematode disease
| Seedling substrate for test | Quantity of matrix nematodes for seedling before transplanting (bars/100 g soil) | Number of root knots of individual plants after 30d |
| Example 2 | 341 | 37.67 |
| Comparative example 1 | 329 | 91.00 |
| Comparative example 2 | 334 | 72.40 |
As shown in Table 2, compared with the single use of fluopyram or Eimeria brevis, the seedling substrate of the invention can effectively defend the effect of the meloidogyne incognita on the seedlings of crops.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (4)
1. The seedling substrate is characterized by being prepared by mixing the following components in percentage by mass: 90-95% of matrix component, 0.5-0.75% of biological microbial inoculum component and the balance of fertilizer component; wherein, the matrix components are composed of vermiculite, perlite, peat soil and coconut coir according to the weight ratio of 4:1:1:2, mixing the materials according to the mass ratio;
the biological agent is prepared by mixing Equilibrium brevifolium and fluopyram;
the fertilizer comprises the following components of potassium dihydrogen phosphate, potassium sulfate and magnesium sulfate according to the proportion of 8:4:3 by mass ratio.
2. The seedling raising substrate of claim 1, wherein the stationary bacillus brevis is 300 hundred megaspores/gram of stationary bacillus brevis parent drug; the fluopyram suspension agent comprises 41.7% of fluopyram by mass fraction.
3. The seedling raising substrate as claimed in claim 1, wherein the mass ratio of the Eimeria brevis to the fluopyram is 1-7:19-1.
4. The seedling raising substrate as set forth in claim 1, wherein the mass ratio of the Eimeria brevis to the fluopyram is 1:7.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310366799.5A CN116349576A (en) | 2023-04-07 | 2023-04-07 | Seedling substrate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310366799.5A CN116349576A (en) | 2023-04-07 | 2023-04-07 | Seedling substrate |
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| CN116349576A true CN116349576A (en) | 2023-06-30 |
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| CN202310366799.5A Pending CN116349576A (en) | 2023-04-07 | 2023-04-07 | Seedling substrate |
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