CN116602307A - Pesticide composition for preventing and controlling root-knot nematode of momordica grosvenori - Google Patents
Pesticide composition for preventing and controlling root-knot nematode of momordica grosvenori Download PDFInfo
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- 241000243785 Meloidogyne javanica Species 0.000 title claims abstract description 28
- 239000000203 mixture Substances 0.000 title claims abstract description 24
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title claims abstract description 19
- 239000000575 pesticide Substances 0.000 title claims abstract description 19
- 230000003405 preventing effect Effects 0.000 title claims abstract description 14
- 241001409321 Siraitia grosvenorii Species 0.000 title claims abstract 5
- AZSNMRSAGSSBNP-UHFFFAOYSA-N 22,23-dihydroavermectin B1a Natural products C1CC(C)C(C(C)CC)OC21OC(CC=C(C)C(OC1OC(C)C(OC3OC(C)C(O)C(OC)C3)C(OC)C1)C(C)C=CC=C1C3(C(C(=O)O4)C=C(C)C(O)C3OC1)O)CC4C2 AZSNMRSAGSSBNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 229960002418 ivermectin Drugs 0.000 claims abstract description 35
- SPBDXSGPUHCETR-JFUDTMANSA-N 8883yp2r6d Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O[C@@H]([C@@H](C)CC4)C(C)C)O3)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1C[C@H](C)[C@@H]([C@@H](C)CC)O[C@@]21O[C@H](C\C=C(C)\[C@@H](O[C@@H]1O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C1)[C@@H](C)\C=C\C=C/1[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\1)O)C[C@H]4C2 SPBDXSGPUHCETR-JFUDTMANSA-N 0.000 claims abstract description 33
- 239000005825 Prothioconazole Substances 0.000 claims abstract description 17
- MNHVNIJQQRJYDH-UHFFFAOYSA-N 2-[2-(1-chlorocyclopropyl)-3-(2-chlorophenyl)-2-hydroxypropyl]-1,2-dihydro-1,2,4-triazole-3-thione Chemical compound N1=CNC(=S)N1CC(C1(Cl)CC1)(O)CC1=CC=CC=C1Cl MNHVNIJQQRJYDH-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000005867 Iprodione Substances 0.000 claims abstract description 16
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 claims abstract description 14
- CWFOCCVIPCEQCK-UHFFFAOYSA-N chlorfenapyr Chemical compound BrC1=C(C(F)(F)F)N(COCC)C(C=2C=CC(Cl)=CC=2)=C1C#N CWFOCCVIPCEQCK-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241000243786 Meloidogyne incognita Species 0.000 abstract description 17
- 239000003814 drug Substances 0.000 abstract description 10
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- 238000013329 compounding Methods 0.000 abstract description 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 206010059866 Drug resistance Diseases 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
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- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- AZSNMRSAGSSBNP-XPNPUAGNSA-N 22,23-dihydroavermectin B1a Chemical compound C1C[C@H](C)[C@@H]([C@@H](C)CC)O[C@@]21O[C@H](C\C=C(C)\[C@@H](O[C@@H]1O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C1)[C@@H](C)\C=C\C=C/1[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\1)O)C[C@H]4C2 AZSNMRSAGSSBNP-XPNPUAGNSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
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- -1 iprodione compound Chemical class 0.000 description 1
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- 239000005645 nematicide Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling Siraitia grosvenorii root-knot nematodes. The pesticide composition for preventing and controlling the root-knot nematode of the momordica grosvenori is prepared by compounding ivermectin and chlorfenapyr, iprodione or prothioconazole. When the effective components of the nematicidal composition are compounded, the co-toxicity coefficient of the nematicidal composition for the second-instar larvae of the meloidogyne incognita is more than 120, the synergistic effect is shown, the control effect on the meloidogyne incognita can be improved, and further, the support can be provided for developing and developing high-efficiency medicaments for controlling the meloidogyne incognita.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling Siraitia grosvenorii root-knot nematodes.
Background
Fructus Siraitiae Grosvenorii fruit has effects of clearing heat and detoxicating, moistening lung and relieving asthma, eliminating phlegm and relieving cough, refreshing and promoting fluid production, and has certain effects of preventing and relieving diabetes, treating coronary heart disease, etc. For a long time, in the planting process, the root of the momordica grosvenori is easy to be affected by root knot nematode, so that growths of momordica grosvenori plants and fruits are poor, the result quantity is greatly reduced, and the economic benefit of planting the momordica grosvenori is influenced. Researches report that the incidence rate of the root-knot nematodes of the momordica grosvenori reaches more than 86% under the condition that nematicides are not used, and after the momordica grosvenori plants are affected by the root-knot nematodes, soil-borne diseases such as fusarium wilt, root rot and the like are serious, so that compound invasion is caused. The yield of the momordica grosvenori can be reduced by 60-70% under the influence of the invasion of the root knot nematode disease, and the development of the momordica grosvenori industry is severely restricted.
In Guangxi, the Siraitia grosvenorii root-knot nematodes are mainly southern root-knot nematodes (Meloidogyne incognita) and Java root-knot nematodes (Meloidogyne javanica), wherein southern root-knot nematodes are dominant populations. The chemical agent is an effective and convenient way for preventing and controlling the root-knot nematode, but the drug resistance of pests is rapidly accumulated due to the unreasonable use of a single chemical agent for a long time, so that the preventing and controlling effect of the chemical agent on the pests is reduced. The screening of the compound formula with synergistic effect on the root-knot nematodes can provide support for developing and developing efficient medicaments for preventing and treating the root-knot nematodes.
Ivermectin, alias: ivermectin, CAS:70288-86-7, molecular formula: c (C) 48 H 74 O 14 The structure is as follows
The inventor finds that when ivermectin and chlorfenapyr, iprodione or prothioconazole are compounded through indoor experiments, the ivermectin and iprodione compound pesticide has a synergistic effect on southern root-knot nematodes, and can provide support for developing and developing high-efficiency medicaments for preventing and treating the root-knot nematodes.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a pesticide composition for preventing and controlling the root-knot nematode of Momordica grosvenori, which solves the problem that the prevention and control effect of a chemical agent on pests is reduced because the drug resistance of the pests is rapidly accumulated due to unreasonable use of the single chemical agent for a long time.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the pesticide composition for preventing and controlling the root-knot nematode of the momordica grosvenori is prepared by compounding ivermectin and chlorfenapyr, iprodione or prothioconazole.
Further, the mass ratio of the ivermectin to the chlorfenapyr is 1-13:22-1.
Further, the mass ratio of ivermectin to iprodione is 1:15-1.
Further, the mass ratio of the ivermectin to the prothioconazole is 1-10:16-1.
The invention also provides application of the pesticide composition in preventing and controlling the root-knot nematode of the momordica grosvenori.
Compared with the prior art, the invention has the following beneficial effects:
(1) When the effective components of the nematicidal composition are compounded, the co-toxicity coefficient of the nematicidal composition for the second-instar larvae of the meloidogyne incognita is more than 120, the synergistic effect is shown, the control effect on the meloidogyne incognita can be improved, and further, the support can be provided for developing and developing high-efficiency medicaments for controlling the meloidogyne incognita.
(2) The nematicidal composition has different nematicidal action mechanisms and can delay the development of the drug resistance of the root-knot nematodes.
Detailed Description
The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: indoor biological Activity assay for root knot nematode
1. Test object: root-knot nematode of south
2. Test agent
94% of ivermectin technical, 97% of chlorfenapyr technical, 96% of iprodione technical and 96% of prothioconazole technical are all sold in the market.
The raw materials are dissolved by dimethyl sulfoxide to prepare single-dose mother liquor, then the mother liquor is diluted by 0.1% Tween-80 aqueous solution, a plurality of groups of proportions are arranged, 5 gradient mass concentrations of each single dose and each group of proportion mixture are arranged according to an equal ratio method, and each mass concentration liquid medicine is 50mL for standby.
3. The test method comprises the following steps: (reference to NT/Y1833.1-2009 methods for testing nematode dipping in the pesticide section 1 method for inhibiting plant pathogenic nematodes by means of biological assay in pesticide chambers)
Picking eggs of root knot nematodes from roots of momordica grosvenori, cleaning with clear water, placing on wet filter paper of a culture dish, and incubating at 25 ℃ to obtain second-instar larvae with consistent instar; eluting the cultured root-knot nematode with water, filtering, centrifuging at 1000r/min for 2min, discarding supernatant, adding water, centrifuging again, and finally re-suspending the root-knot nematode with water to 150 head/mL for use. 3mL of liquid medicine is sucked by a pipette and added into a test tube, then 3mL of nematode suspension is sucked and added into the test tube, and the liquid medicine and the nematode suspension are uniformly mixed in equal quantity; transferring a certain volume of the mixed solution into the small holes of a 24-hole biochemical test plate by using a liquid transfer device, covering, and culturing for 24 hours at a constant temperature of 25 ℃; a blank was also prepared from a 0.1% Tween-80 aqueous solution.
Taking 1mL of mixed solution from each treatment, observing the death condition of nematodes under an dissecting lens, and recording the number of bus worms and the number of dead nematodes for investigation when the number of nematodes observed per repetition is not less than 100. Wherein the criterion for nematode death is: the nematodes are stiff and cannot bend when touched by a hairline needle or a bamboo filament needle; from the survey data, mortality and corrected mortality for each treatment were calculated.
In the above formula: p- -mortality in units of; k- -number of dead insects; n- -total number of insects treated.
In the above formula: p (P) 1 -correct mortality in units of; p (P) t -mortality rate in units of treatment; p (P) 0 Blank mortality in%.
4. Data analysis: regression analysis of the log concentration values of each treatment agent and the corrected mortality probability values of each treatment was performed using DPS software to calculate LC of each treatment agent 50 And calculating co-toxicity coefficient (CTC value) of the mixture according to grand cloud Pei method)。
5. Results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-3.
TABLE 1 indoor biological Activity of ivermectin and chlorfenapyr combination against Nannoophorite
As can be seen from Table 1, at 1-13:22-1, the co-toxicity coefficient of ivermectin and chlorfenapyr to the second instar root-knot nematode is more than 120, and the synergistic effect is shown.
TABLE 2 indoor biological Activity of ivermectin and iprodione combinations against meloidogyne incognita
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Ivermectin | 5.5631 | 100.0000 | -- | -- |
| Iprodione | 17.8587 | 31.1506 | -- | -- |
| Ivermectin 1: iprodione 15 | 12.8091 | 43.4308 | 35.4537 | 122.5001 |
| Ivermectin 1: iprodione 10 | 9.6218 | 57.8177 | 37.4097 | 154.5527 |
| Ivermectin 1: iprodione 5 | 8.8057 | 63.1761 | 42.6255 | 148.2119 |
| Ivermectin 1: iprodione 3 | 9.3113 | 59.7457 | 48.3630 | 123.5360 |
| Ivermectin 1: iprodione 1 | 7.0091 | 79.3697 | 65.5753 | 121.0359 |
As can be seen from table 2, at 1: within the mass ratio of 15-1, the co-toxicity coefficient of ivermectin and iprodione to the second age of the meloidogyne incognita is more than 120, and the synergistic effect is shown.
TABLE 3 indoor biological Activity of ivermectin and prothioconazole combination against meloidogyne incognita
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Ivermectin | 5.5631 | 100.0000 | -- | -- |
| Prothioconazole | 9.0624 | 61.3866 | -- | -- |
| Ivermectin 1: propylthioMyclobutanil 16 | 6.2225 | 89.4030 | 63.6580 | 140.4427 |
| Ivermectin 1: prothioconazole 12 | 3.8079 | 146.0936 | 64.3569 | 227.0055 |
| Ivermectin 1: prothioconazole 8 | 5.5491 | 100.2523 | 65.6770 | 152.6445 |
| Ivermectin 1: prothioconazole 4 | 6.1007 | 91.1879 | 69.1093 | 131.9474 |
| Ivermectin 1: prothioconazole 1 | 5.0091 | 111.0599 | 80.6933 | 137.6321 |
| Ivermectin 4: prothioconazole 1 | 4.1747 | 133.2575 | 92.2773 | 144.4098 |
| Ivermectin 8: prothioconazole 1 | 3.3767 | 164.7496 | 95.7096 | 172.1348 |
| Ivermectin 10: prothioconazole 1 | 1.9862 | 280.0876 | 96.4897 | 290.2772 |
As can be seen from Table 3, at 1-10: within the mass ratio of 16-1, the co-toxicity coefficient of ivermectin and prothioconazole to the second instar of the meloidogyne incognita is more than 120, and the synergistic effect is shown.
In conclusion, when the effective components of the nematicidal composition are compounded, the co-toxicity coefficient of the nematicidal composition for the second-instar larvae of the meloidogyne incognita is more than 120, and the nematicidal composition has a synergistic effect, can improve the control effect of the meloidogyne incognita, and further can provide support for developing and developing efficient medicaments for controlling the meloidogyne incognita.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (5)
1. The pesticide composition for preventing and controlling the root-knot nematode of the momordica grosvenori is characterized in that the effective components of the pesticide composition are compounded by ivermectin and chlorfenapyr, iprodione or prothioconazole.
2. The pesticide composition as set forth in claim 1, wherein the mass ratio of ivermectin to chlorfenapyr is 1-13:22-1.
3. The pesticide composition as set forth in claim 1, wherein the mass ratio of ivermectin to iprodione is 1:15-1.
4. A pesticide composition as set forth in claim 1, characterized in that the mass ratio of ivermectin to prothioconazole is 1-10:16-1.
5. Use of a pesticide composition as set forth in any one of claims 1 to 4 for controlling root-knot nematodes in momordica grosvenori.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310633608.7A CN116602307A (en) | 2023-05-31 | 2023-05-31 | Pesticide composition for preventing and controlling root-knot nematode of momordica grosvenori |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310633608.7A CN116602307A (en) | 2023-05-31 | 2023-05-31 | Pesticide composition for preventing and controlling root-knot nematode of momordica grosvenori |
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