CN116602308A - Bactericide composition for preventing and treating bacterial wilt of momordica grosvenori - Google Patents
Bactericide composition for preventing and treating bacterial wilt of momordica grosvenori Download PDFInfo
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- CN116602308A CN116602308A CN202310633692.2A CN202310633692A CN116602308A CN 116602308 A CN116602308 A CN 116602308A CN 202310633692 A CN202310633692 A CN 202310633692A CN 116602308 A CN116602308 A CN 116602308A
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- aureonucleomycin
- bacterial wilt
- momordica grosvenori
- bactericide composition
- preventing
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- 230000001580 bacterial effect Effects 0.000 title claims abstract description 25
- 239000000203 mixture Substances 0.000 title claims abstract description 21
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title claims abstract description 18
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 17
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 17
- 241001409321 Siraitia grosvenorii Species 0.000 title claims abstract 7
- WHHIPMZEDGBUCC-UHFFFAOYSA-N probenazole Chemical compound C1=CC=C2C(OCC=C)=NS(=O)(=O)C2=C1 WHHIPMZEDGBUCC-UHFFFAOYSA-N 0.000 claims abstract description 13
- FXVNBZGTAWLLNE-UHFFFAOYSA-N 1,3-thiazole;zinc Chemical compound [Zn].C1=CSC=N1 FXVNBZGTAWLLNE-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000013329 compounding Methods 0.000 claims abstract description 4
- 241000589771 Ralstonia solanacearum Species 0.000 claims description 12
- 208000035143 Bacterial infection Diseases 0.000 claims description 4
- 208000022362 bacterial infectious disease Diseases 0.000 claims description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 9
- 244000052769 pathogen Species 0.000 abstract description 8
- 239000000575 pesticide Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 239000004480 active ingredient Substances 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 12
- 244000185386 Thladiantha grosvenorii Species 0.000 description 11
- 239000003814 drug Substances 0.000 description 8
- 230000004071 biological effect Effects 0.000 description 7
- IOJUPLGTWVMSFF-UHFFFAOYSA-N cyclobenzothiazole Natural products C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- -1 allyl benzothiazole Chemical compound 0.000 description 6
- 239000002609 medium Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 206010059866 Drug resistance Diseases 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 239000013043 chemical agent Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 235000009815 Momordica Nutrition 0.000 description 2
- 241000218984 Momordica Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000447 pesticide residue Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241000186984 Kitasatospora aureofaciens Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000002888 effect on disease Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating bacterial wilt of momordica grosvenori. A bactericide composition for preventing and treating the bacterial wilt of momordica grosvenori is prepared by compounding aureonucleomycin and shenzinmycin, probenazole or zinc thiazole. After the two active ingredients are compounded, the bactericide composition has a synergistic effect on target bacterial wilt pathogens of momordica grosvenori, and can improve the control effect on the target bacterial wilt pathogens.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericide composition for preventing and treating bacterial wilt of momordica grosvenori.
Background
Bacterial wilt of momordica grosvenori is a bacterial disease caused by infection of ralstonia solanacearum (Ralstonia solanacearum), which is mainly the base and stem of harmful plants. In the early stage of disease, the basal epidermis of the plant is rough and easy to crack, the stem vascular bundles turn brown, and the diseased leaves have the phenomenon of fading green. The disease plant is first top leaf withered to droop, then the whole leaf withers, the disease course develops rapidly, and the disease plant dies in a few days. The bacterial wilt of the momordica grosvenori can be prevented by spraying the chemical agent, but the single chemical agent is used for a long time, so that the problems of drug resistance, pesticide residue, environmental pollution and the like are easily caused, and meanwhile, the prevention and control effect on diseases is reduced. The compound formula can delay the generation and development of the drug resistance of diseases and improve the control effect on the diseases, so that the screening of the compound formula with the synergistic effect on the bacterial wilt of the momordica grosvenori has great significance.
The aureonucleomycin is a nucleoside antibiotic containing adenine and a special undecanose skeleton, is separated from fermentation liquor of streptomyces aureofaciens SP-371 by Shanghai city pesticide institute, and has good control effect on bacterial diseases such as citrus canker, rice bacterial leaf blight and the like. The structural formula of aureonuclycin is as follows:
the inventor screens out the compound formulation of aureonucleomycin and shenqimycin, probenazole or thiazole zinc through a large number of indoor tests, and the related compound formulation has synergism on targets in a certain range. No relevant report is currently seen in this regard.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a bactericide composition for preventing and treating the bacterial wilt of momordica grosvenori, so as to solve the problem of long-term use of a single chemical agent.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a bactericide composition for preventing and treating the bacterial wilt of momordica grosvenori is prepared by compounding aureonucleomycin and shenzinmycin, probenazole or zinc thiazole.
More specifically, the mass ratio of the aureonucleomycin to the shenzinomycin is 1-20:20-1.
More specifically, the mass ratio of the aureonucleomycin to the probenazole is 1-8:30-1.
More specifically, the mass ratio of the aureonucleomycin to the zinc thiazole is 1-5:9-1.
More specifically, the mass ratio of the aureonucleomycin to the zinc thiazole is 5:1.
the invention also provides application of the pesticide composition in preventing and treating the bacterial wilt of the momordica grosvenori.
More specifically, the bacterial wilt of the momordica grosvenori is caused by bacterial infection of Ralstonia solanacearum.
Compared with the prior art, the invention has the following beneficial effects:
(1) After the two active ingredients are compounded, the bactericide composition has a synergistic effect on target bacterial wilt pathogens of momordica grosvenori, and can improve the control effect on the target bacterial wilt pathogens.
(2) The two active ingredients of the bactericide composition have different action mechanisms, and can delay the generation and development of drug resistance of pathogenic bacteria.
(3) The bactericide composition can reduce the application dosage of pesticides, reduce the disease control cost, reduce pesticide residues and reduce environmental pollution.
Detailed Description
The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: indoor biological activity test of aureonuclycin after compounding
1. Test pathogen: and (5) separating and purifying a bacterial wilt sample of the grosvenor momordica in the grosvenor momordica plantation in the Guangxi province by using Ralstonia solanacearum (Ralstonia solanacearum).
Culturing test bacteria in a bacterial incubator at 30deg.C for 48 hr, picking single colony in 30mLNB medium, shake culturing at 30deg.C for 120r/min until test bacteria reach logarithmic phase, and regulating bacterial suspension concentration to 1×10 with sterile water 7 cfu/mL for use.
2. Culture medium
NA medium: 6g of polypeptone, 1g of yeast powder, 3g of beef extract, 15g of sucrose and 17g of agar, adding distilled water to a volume of 1L, and adjusting the pH to 7.0 by using 10mol/L NaOH.
NB medium: 6g of polypeptone, 1g of yeast powder, 3g of beef extract and 15g of sucrose, adding distilled water to a volume of 1L, and adjusting the pH to 7.0 by using 10mol/L NaOH.
3. Test agent
94% of aureonucleomycin technical, 95% of shenzinmycin technical, 95% of probenazole technical and 95% of zinc thiazole technical are all sold in the market.
Dissolving the test agent with dimethyl sulfoxide, and diluting with tween-80 water solution with mass fraction of 0.1% to obtain single-agent mother solution. And setting a plurality of groups of proportions, and setting 5 series of mass concentration gradients for each single dose and each mixed dose, wherein each mass concentration of the liquid medicine is 50mL for standby.
4. Test method (refer to NT/T1156.16-2008 pesticide laboratory biological assay test criteria section 16. Turbidity method for inhibition of bacterial growth test)
Under aseptic conditions, the liquid medicine is quantitatively sucked from low concentration to high concentration in sequence and added into 30mL of NB medium, and a treatment of adding sterile water is used as a blank control, and 4 replicates are arranged for each treatment. Then, 100. Mu.L of the test pathogen suspension was inoculated into each treated NB medium, and the culture was performed under shaking at 30℃and 120 r/min.
And respectively measuring the turbidity of each treatment before starting culture, and when the blank control treatment test pathogen reaches the logarithmic growth phase, respectively measuring the turbidity of each treatment again, and calculating the growth inhibition rate of the test pathogen.
4. Data analysis: performing linear regression analysis with the logarithmic value of the concentration of the drug as x and the corresponding value of the growth inhibition rate as y, and calculating the EC of each treatment drug 50 And the co-toxicity coefficient (CTC value) of the mixture was calculated according to the grand cloud Pei method.
And evaluating the synergy of the mixed medicaments according to the co-toxicity coefficient (CTC), wherein CTC is less than or equal to 80 and is antagonistic, CTC is less than or equal to 80 and is less than 120 and is additive, and CTC is more than or equal to 120 and is synergistic. The experimental results are shown in tables 1-3.
TABLE 1 determination of indoor biological Activity of aureonuclein and Shenqimycin Complex against Ralstonia solanacearum
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Aureonucleomycin | 6.4135 | 100.0000 | -- | -- |
| Shenqimycin | 20.4526 | 31.3579 | -- | -- |
| Aureonucleomycin 1: shenqimycin 20 | 14.7677 | 43.4292 | 34.6265 | 125.4218 |
| Aureonucleomycin 1: shenqimycin 15 | 12.6082 | 50.8677 | 35.6480 | 142.6944 |
| Aureonucleomycin 1: shenqimycin 10 | 10.0170 | 64.0262 | 37.5981 | 170.2911 |
| Aureonucleomycin 1: shenqimycin 5 | 7.4664 | 85.8982 | 42.7982 | 200.7049 |
| Aureonucleomycin 1: shenqimycin 1 | 5.9961 | 106.9612 | 65.6789 | 162.8546 |
| Aureonuclycin 5: shenqimycin 1 | 3.8876 | 164.9732 | 88.5596 | 186.2849 |
| Aureonuclycin 10: shenqimycin 1 | 4.2025 | 152.6115 | 93.7598 | 162.7686 |
| Aureonuclycin 15: shenqimycin 1 | 5.2281 | 122.6736 | 95.7099 | 128.1724 |
| Aureonuclycin 20: shenqimycin 1 | 5.4374 | 117.9516 | 96.7313 | 121.9373 |
As can be seen from Table 1, the mass ratio of aureonucleomycin to shenzinomycin is 1-20: in the range of 20-1, the co-toxicity coefficient is more than 120, namely the biological activity of the aureonucleomycin and the shenzinomycin to the ralstonia solanacearum after being compounded shows a synergistic effect.
TABLE 2 determination of indoor biological Activity of aureonuclycin and probenazole complex against Ralstonia solanacearum
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Aureonucleomycin | 6.4135 | 100.0000 | -- | -- |
| Allyl benzothiazole | 11.0854 | 57.8554 | -- | -- |
| Aureonucleomycin 1: allyl benzothiazole 30 | 6.0272 | 106.4093 | 59.2149 | 179.7002 |
| Aureonucleomycin 1: allyl benzothiazole 20 | 8.3137 | 77.1438 | 59.8623 | 128.8687 |
| Aureonucleomycin 1: allyl benzothiazole 10 | 4.9281 | 130.1414 | 61.6867 | 210.9716 |
| Aureonucleomycin 1: probenazole 5 | 7.1248 | 90.0166 | 64.8795 | 138.7443 |
| Aureonucleomycin 1: allyl benzothiazole 1 | 6.6322 | 96.7025 | 78.9277 | 122.5203 |
| Aureonuclycin 5: allyl benzothiazole 1 | 4.3695 | 146.7788 | 92.9759 | 157.8676 |
| Aureonuclycin 8: allyl benzothiazole 1 | 5.5248 | 116.0857 | 95.3173 | 121.7887 |
As can be seen from Table 2, the mass ratio of aureonucleomycin to probenazole is 1-8: within the range of 30-1, the co-toxicity coefficient is larger than 120, namely the biological activity of the aureonucleomycin and the probenazole after being compounded on the ralstonia solanacearum shows a synergistic effect.
TABLE 3 determination of indoor biological Activity of aureonuclein and Shenqimycin Complex against Ralstonia solanacearum
| Medicament name and ratio | EC 50 (mg/L) | ATI | TTI | CTC |
| Aureonucleomycin | 6.4135 | 100.0000 | -- | -- |
| Shenqimycin | 2.7347 | 234.5230 | -- | -- |
| Aureonucleomycin 1: shenqimycin 9 | 1.0547 | 608.0876 | 221.0707 | 275.0648 |
| Aureonucleomycin 1: shenqimycin 5 | 0.8023 | 799.3893 | 212.1025 | 376.8882 |
| Aureonucleomycin 1: shenqimycin 3 | 2.1448 | 299.0256 | 200.8922 | 148.8487 |
| Aureonucleomycin 1: shenqimycin 1 | 3.1124 | 206.0628 | 167.2615 | 123.1980 |
| Aureonuclycin 3: shenqimycin 1 | 1.8781 | 341.4887 | 133.6307 | 255.5465 |
| Aureonuclycin 5: shenqimycin 1 | 1.2156 | 527.5995 | 122.4205 | 430.9732 |
As can be seen from Table 3, the mass ratio of aureonucleomycin to shenzinomycin is 1-5: in the range of 9-1, the co-toxicity coefficient is more than 120, namely the biological activity of the aureonucleomycin and the shenzinomycin to the ralstonia solanacearum after being compounded shows synergistic effect, especially when the mass ratio is 5: in the 1, the co-toxicity coefficient reaches 430.9732, and the synergistic effect is most remarkable.
In conclusion, when the aureonucleomycin, the shenqimycin, the probenazole or the zinc thiazole are compounded within a certain mass ratio range, the co-toxicity coefficients of the aureonucleomycin and the Shenqimycin, the probenazole or the zinc thiazole are higher than 120, the synergistic effect is shown, the prevention and treatment effect on the bacterial wilt of the momordica grosvenori can be improved, and the drug resistance is not easy to generate.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (7)
1. The bactericide composition for preventing and treating the bacterial wilt of the momordica grosvenori is characterized by being prepared by compounding aureonucleomycin, shenzinmycin, probenazole or zinc thiazole.
2. The bactericide composition according to claim 1, characterized in that the mass ratio of aureonucleomycin and shenzinomycin is 1-20:20-1.
3. The bactericide composition according to claim 1, characterized in that the mass ratio of aureonucleomycin and probenazole is 1-8:30-1.
4. The bactericide composition according to claim 1, characterized in that the mass ratio of aureonucleomycin to zinc thiazole is 1-5:9-1.
5. The bactericide composition according to claim 1, characterized in that the mass ratio of aureonucleomycin to zinc thiazole is 5:1.
6. use of the bactericide composition according to any one of claims 1-5 for controlling bacterial wilt of momordica grosvenori.
7. The use according to claim 6, wherein said bacterial wilt of momordica grosvenori is caused by bacterial infection with ralstonia solanacearum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310633692.2A CN116602308A (en) | 2023-05-31 | 2023-05-31 | Bactericide composition for preventing and treating bacterial wilt of momordica grosvenori |
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| CN202310633692.2A CN116602308A (en) | 2023-05-31 | 2023-05-31 | Bactericide composition for preventing and treating bacterial wilt of momordica grosvenori |
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