CN114847297B - Pesticide composition for preventing and treating rice diseases - Google Patents

Pesticide composition for preventing and treating rice diseases Download PDF

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Publication number
CN114847297B
CN114847297B CN202210324204.5A CN202210324204A CN114847297B CN 114847297 B CN114847297 B CN 114847297B CN 202210324204 A CN202210324204 A CN 202210324204A CN 114847297 B CN114847297 B CN 114847297B
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pyraclostrobin
parthenolide
rice
pesticide composition
preventing
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CN114847297A (en
Inventor
禹震洋
黄秋榕
张德平
林繁东
林纬
张得平
唐德剑
潘忠银
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Jiangsu Hwanon Biochemistry Co ltd
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Zhengzhou Fudao Ecological Agriculture Technology Co ltd
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Priority to CN202310676620.6A priority Critical patent/CN116616302A/en
Priority to CN202210324204.5A priority patent/CN114847297B/en
Priority to CN202310676618.9A priority patent/CN116671520A/en
Priority to CN202310676616.XA priority patent/CN116616305A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating rice diseases. The pesticide composition for preventing and treating rice diseases is prepared by compounding pyraclostrobin, enestroburin, pyraclostrobin or thifluzamide and parthenolide in a mass ratio of 1-200:200-1. When the active ingredients of the pyraclostrobin, the enestroburin, the pyraclostrobin or the thifluzamide and the parthenolide are compounded, the co-toxicity coefficient is more than 120 within a certain mass ratio range, and the synergistic effect is shown. Based on the above, the pesticide composition can effectively control rice sheath blight, can improve the control effect on rice sheath blight compared with a single medicament, is beneficial to reducing the dosage and the pollution of pesticides to the environment, and can provide support for developing novel pesticides.

Description

Pesticide composition for preventing and treating rice diseases
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating rice diseases.
Background
Rhizoctonia solani is a fungal disease caused by Rhizoctonia solani (Rhizoctonia solani), and is one of the important rice diseases in the world. The rice sheath blight disease can occur in the whole growth period of the rice, generally, the disease is heaviest from the beginning of the tillering period to the beginning and the end of heading, leaf sheaths and leaves are endangered, the scion and the stalk are endangered when serious, the disease causes the reduction of the seed setting rate of the rice, the thousand grain weight is reduced, and the yield of the rice is seriously influenced.
The common medicaments for preventing and treating rice sheath blight disease in agricultural production include validamycin, triazolone, hexaconazole and the like, but the long-time use of a single medicament for preventing and treating diseases greatly increases the risk of pathogen resistance, and causes a series of problems of annual decline of prevention effect, pathogen resistance synergism, ecological environment pollution and the like.
The pesticide mixed use is one of effective measures for delaying the drug resistance of agricultural pests. Different pesticides are compounded, so that the generation of pesticide resistance of pests can be delayed, the control effect is improved, the dosage is reduced, and the pollution of the pesticides to the environment is reduced.
CN201610138008.3 discloses a preparation method and application of a pesticide bactericide containing parthenolide, and in particular discloses a preparation method and application of parthenolide to various pathogenic bacteria such as sheath blight germ, sclerotinia rot of rice, sheath blight germ of wheat, small spot germ of corn, rice blast germ, bacterial leaf blight germ of rice, bacterial leaf spot germ of rice and the like, wherein the parthenolide has good efficacy to EC of sheath blight germ of rice 50 36.83mg/L.
At present, no report on the compounding of pyraclostrobin, enestroburin, pyraclostrobin or thifluzamide and parthenolide is found.
Disclosure of Invention
The invention aims to provide a pesticide composition for preventing and treating rice diseases, which solves the problems of reduced prevention effect, enhanced drug resistance, ecological environment pollution and the like caused by long-time use of a single medicament.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the pesticide composition for preventing and treating rice diseases is prepared by compounding pyraclostrobin, enestroburin, pyraclostrobin or thifluzamide and parthenolide in a mass ratio of 1-200:200-1.
Preferably, the mass ratio of the pyraclostrobin to the parthenolide is 1:35-1.
Preferably, the mass ratio of the enestroburin to the parthenolide is 1-5:20-1.
Preferably, the mass ratio of the pyraclostrobin to the parthenolide is 1-30:10-1.
Preferably, the mass ratio of the thifluzamide to the parthenolide is 1-10:45-1.
Preferably, the rice disease is rice sheath blight.
Compared with the prior art, the invention has the following beneficial effects:
when the active ingredients of the pyraclostrobin, the enestroburin, the pyraclostrobin or the thifluzamide and the parthenolide are compounded, the co-toxicity coefficient is more than 120 within a certain mass ratio range, and the synergistic effect is shown. Based on the above, the pesticide composition can effectively control rice sheath blight, can improve the control effect on rice sheath blight compared with a single medicament, is beneficial to reducing the dosage and the pollution of pesticides to the environment, and can provide support for developing novel pesticides.
Detailed Description
The invention will be better understood from the following examples. However, it will be readily appreciated by those skilled in the art that the description of the embodiments is provided for illustration only and should not limit the invention as described in detail in the claims.
Examples: screening of rice sheath blight disease control agent
1. Test strain: rhizoctonia solani (Rhizoctonia solani) was isolated and purified in laboratory from a contaminated rice plant in suburban rice fields in Zhengzhou Henan and stored on PDA medium. ( PDA culture medium formula: peeled potato 200g, sucrose 20g, agar 18g, water 1L, natural pH )
2. Test agent: 98% pyraclostrobin (Canon Black Dragon pesticide Co., ltd.), 90% enestroburin (Shenyang Co., ltd.), 99% pyraclostrobin (Tianjin Alta technologies Co., ltd.), 96% thifluzamide (Zhejiang Tianfeng biosciences Co., ltd.), 98% parthenolide (Shanghai Altin biosciences Co., ltd.).
Dissolving the test reagent with dimethyl sulfoxide solvent, diluting with 0.1% Tween-80 aqueous solution to obtain single-dose mother solution, and setting multiple groups of ratios, wherein each single dose and the ratio mixture have 5 mass concentration gradients according to an equal ratio method.
3. Test method (refer to "pesticide laboratory biological assay test criteria Bactericide part 2: petri dish method for inhibiting growth of pathogenic fungi" Petri dish method)
Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1.5mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, shaking uniformly, and pouring into 3 culture dishes with the diameter of 9cm in equal quantity to prepare a medicine-containing flat plate with the corresponding concentration; treatments with only 0.1% tween-80 in water were also included as blank, and 3 replicates were set for each treatment.
Cutting bacterial cake at the colony edge of the tested bacterial strain by using a puncher with the diameter of 5mm, inoculating the bacterial cake to the centers of a medicine-containing flat plate and a blank control flat plate, covering a dish cover, and then placing the dish cover at the constant temperature of 25 ℃ for culture. After 48h, colony growth diameter was measured by crisscross method and the inhibition of hypha growth by different treatments was calculated.
4. Data analysis: performing data statistical analysis by DPS software, and linearly regressing with the value of the reagent concentration logarithmic value x and the value of the corresponding hypha growth inhibition rate numerical value y to obtain a virulence regression equation and the virulence EC of the reagent on the target pathogen 50 Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.
Measured virulence index (ATI) = (standard agent EC 50/test agent EC 50) ×100;
theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture;
co-toxicity coefficient (CTC) = [ actual drug susceptibility index (ATI)/theoretical drug susceptibility index (TTI) ] x 100.
5. Measurement results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-4.
TABLE 1 toxicity determination results of pyraclostrobin and parthenolide compounded Rhizoctonia solani
As can be seen from table 1, at 1:35-1, wherein the co-toxicity coefficient of the pyraclostrobin and the parthenolide after being compounded is greater than 120, and the synergistic effect is shown.
TABLE 2 toxicity determination results of enestroburin and parthenolide compounded Rhizoctonia solani
As can be seen from table 2, at 1-5: within the mass ratio range of 20-1, the co-toxicity coefficient of the compounded enestroburin and the compounded parthenolide to the rhizoctonia solani is more than 120, and the synergistic effect is shown.
TABLE 3 toxicity determination results of pyraclostrobin and parthenolide compounded Rhizoctonia solani
As can be seen from Table 3, at 1-30: within the mass ratio range of 10-1, the co-toxicity coefficient of the pyraclostrobin and the parthenolide after being compounded is greater than 120, and the synergistic effect is shown.
TABLE 4 toxicity determination results of thifluzamide and parthenolide compounded Rhizoctonia solani
As can be seen from Table 4, at 1-10: within the mass ratio range of 45-1, the co-toxicity coefficient of the thifluzamide and the parthenolide after being compounded is greater than 120, and the synergistic effect is shown.
In conclusion, when pyraclostrobin, enestroburin, pyraclostrobin or thifluzamide and parthenolide are compounded, the co-toxicity coefficient is more than 120 within a certain mass ratio range, and the synergistic effect is shown. Based on the above, the pesticide composition can effectively control rice sheath blight, can improve the control effect on rice sheath blight compared with a single medicament, is beneficial to reducing the dosage and the pollution of pesticides to the environment, and can provide support for developing novel pesticides.
The foregoing is merely a preferred embodiment of the present invention, and it will be apparent to those skilled in the art that modifications may be made without departing from the scope of the invention.

Claims (2)

1. The pesticide composition for preventing and treating rice diseases is characterized in that the effective components of the pesticide composition are compounded by pyraclostrobin and parthenolide, and the mass ratio of the pyraclostrobin to the parthenolide is 1:35-1.
2. The pesticidal composition for controlling a rice disease according to claim 1, wherein the rice disease is rice sheath blight.
CN202210324204.5A 2022-03-28 2022-03-28 Pesticide composition for preventing and treating rice diseases Active CN114847297B (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN202310676620.6A CN116616302A (en) 2022-03-28 2022-03-28 Bactericidal active agent for resisting field diseases
CN202210324204.5A CN114847297B (en) 2022-03-28 2022-03-28 Pesticide composition for preventing and treating rice diseases
CN202310676618.9A CN116671520A (en) 2022-03-28 2022-03-28 Low-residue environment-friendly bactericide for resisting field diseases
CN202310676616.XA CN116616305A (en) 2022-03-28 2022-03-28 High-activity bactericide for preventing and treating field diseases

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CN202210324204.5A CN114847297B (en) 2022-03-28 2022-03-28 Pesticide composition for preventing and treating rice diseases

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CN202310676620.6A Division CN116616302A (en) 2022-03-28 2022-03-28 Bactericidal active agent for resisting field diseases
CN202310676618.9A Division CN116671520A (en) 2022-03-28 2022-03-28 Low-residue environment-friendly bactericide for resisting field diseases

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CN202310676618.9A Pending CN116671520A (en) 2022-03-28 2022-03-28 Low-residue environment-friendly bactericide for resisting field diseases
CN202310676620.6A Pending CN116616302A (en) 2022-03-28 2022-03-28 Bactericidal active agent for resisting field diseases

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082321A (en) * 2014-06-09 2014-10-08 武汉科诺生物科技股份有限公司 A sterilization composition and applications thereof in rice disease control
CN105815326A (en) * 2016-03-11 2016-08-03 江苏省中国科学院植物研究所 Preparation method and use of agricultural bactericide containing parthenolide
CN113615700A (en) * 2020-05-09 2021-11-09 江苏省中国科学院植物研究所 Application of parthenolide derivative in prevention and treatment of bacterial blight of rice

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082321A (en) * 2014-06-09 2014-10-08 武汉科诺生物科技股份有限公司 A sterilization composition and applications thereof in rice disease control
CN105815326A (en) * 2016-03-11 2016-08-03 江苏省中国科学院植物研究所 Preparation method and use of agricultural bactericide containing parthenolide
CN113615700A (en) * 2020-05-09 2021-11-09 江苏省中国科学院植物研究所 Application of parthenolide derivative in prevention and treatment of bacterial blight of rice

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CN116616302A (en) 2023-08-22
CN116616305A (en) 2023-08-22
CN114847297A (en) 2022-08-05

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Address after: No. 28 Beiguan Road, Dongtai City, Yancheng City, Jiangsu Province, 224000

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Address before: 450003 east of the 10th floor of the Yellow River hydrological forecasting research building, No. 30, Shuike Road, Jinshui District, Zhengzhou City, Henan Province

Patentee before: Zhengzhou Fudao Ecological Agriculture Technology Co.,Ltd.

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