CN117099789A - Pesticide composition for preventing and treating wheat stem-based rot - Google Patents
Pesticide composition for preventing and treating wheat stem-based rot Download PDFInfo
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- 241000209140 Triticum Species 0.000 title claims abstract description 32
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 32
- 239000000575 pesticide Substances 0.000 title claims abstract description 21
- 239000000203 mixture Substances 0.000 title claims abstract description 18
- VVOAZFWZEDHOOU-UHFFFAOYSA-N honokiol Natural products OC1=CC=C(CC=C)C=C1C1=CC(CC=C)=CC=C1O VVOAZFWZEDHOOU-UHFFFAOYSA-N 0.000 claims abstract description 26
- BYTORXDZJWWIKR-UHFFFAOYSA-N Hinokiol Natural products CC(C)c1cc2CCC3C(C)(CO)C(O)CCC3(C)c2cc1O BYTORXDZJWWIKR-UHFFFAOYSA-N 0.000 claims abstract description 16
- FVYXIJYOAGAUQK-UHFFFAOYSA-N honokiol Chemical compound C1=C(CC=C)C(O)=CC=C1C1=CC(CC=C)=CC=C1O FVYXIJYOAGAUQK-UHFFFAOYSA-N 0.000 claims abstract description 14
- HPUXDMUGCAWDFW-UHFFFAOYSA-N Osthole Natural products COc1ccc2CCC(=O)Oc2c1C=CC(=O)C HPUXDMUGCAWDFW-UHFFFAOYSA-N 0.000 claims abstract description 13
- MBRLOUHOWLUMFF-UHFFFAOYSA-N osthole Chemical compound C1=CC(=O)OC2=C(CC=C(C)C)C(OC)=CC=C21 MBRLOUHOWLUMFF-UHFFFAOYSA-N 0.000 claims abstract description 13
- JWUCHKBSVLQQCO-UHFFFAOYSA-N 1-(2-fluorophenyl)-1-(4-fluorophenyl)-2-(1H-1,2,4-triazol-1-yl)ethanol Chemical compound C=1C=C(F)C=CC=1C(C=1C(=CC=CC=1)F)(O)CN1C=NC=N1 JWUCHKBSVLQQCO-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000005787 Flutriafol Substances 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 4
- 239000004480 active ingredient Substances 0.000 claims abstract description 3
- 241001451172 Fusarium pseudograminearum Species 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 9
- 238000011161 development Methods 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 239000003795 chemical substances by application Substances 0.000 abstract description 3
- 239000003814 drug Substances 0.000 description 11
- 229940079593 drug Drugs 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- 244000052616 bacterial pathogen Species 0.000 description 7
- 239000013043 chemical agent Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000013329 compounding Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000000417 fungicide Substances 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 206010059866 Drug resistance Diseases 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- RFHAOTPXVQNOHP-UHFFFAOYSA-N fluconazole Chemical compound C1=NC=NN1CC(C=1C(=CC(F)=CC=1)F)(O)CN1C=NC=N1 RFHAOTPXVQNOHP-UHFFFAOYSA-N 0.000 description 2
- 229960004884 fluconazole Drugs 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- -1 triazole sterol Chemical class 0.000 description 2
- ZMYFCFLJBGAQRS-IRXDYDNUSA-N (2R,3S)-epoxiconazole Chemical compound C1=CC(F)=CC=C1[C@@]1(CN2N=CN=C2)[C@H](C=2C(=CC=CC=2)Cl)O1 ZMYFCFLJBGAQRS-IRXDYDNUSA-N 0.000 description 1
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 description 1
- MNHVNIJQQRJYDH-UHFFFAOYSA-N 2-[2-(1-chlorocyclopropyl)-3-(2-chlorophenyl)-2-hydroxypropyl]-1,2-dihydro-1,2,4-triazole-3-thione Chemical compound N1=CNC(=S)N1CC(C1(Cl)CC1)(O)CC1=CC=CC=C1Cl MNHVNIJQQRJYDH-UHFFFAOYSA-N 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 239000005767 Epoxiconazole Substances 0.000 description 1
- 241000223194 Fusarium culmorum Species 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 239000005825 Prothioconazole Substances 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 239000005839 Tebuconazole Substances 0.000 description 1
- 239000005842 Thiophanate-methyl Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000017858 demethylation Effects 0.000 description 1
- 238000010520 demethylation reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008686 ergosterol biosynthesis Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000011885 synergistic combination Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical compound COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
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- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
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- Health & Medical Sciences (AREA)
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Abstract
本发明属于农药技术领域，具体涉及一种防治小麦茎基腐病的农药组合物。一种防治小麦茎基腐病的农药组合物，所述农药组合物的有效成分由粉唑醇与厚朴酚或蛇床子素二元复配而成。本发明组合物中粉唑醇与厚朴酚或蛇床子素进行复配时，对引起小麦茎基腐病的假禾谷镰刀菌的共毒系数大于120，表现为协同增效作用，可以提高对小麦茎基腐病的防治效果，可以为开发防治小麦茎基腐病的新型药剂提供支持。The invention belongs to the technical field of pesticides, and specifically relates to a pesticide composition for preventing and treating wheat stem rot. A pesticide composition for preventing and treating wheat stem rot. The active ingredient of the pesticide composition is a binary compound of flutriafol and honokiol or osthole. When the composition of the present invention is compounded with magnolol or osthole, the co-toxicity coefficient against Fusarium pseudograminearum, which causes wheat stem rot, is greater than 120, showing a synergistic effect and can improve The control effect on wheat stem rot can provide support for the development of new agents to prevent and control wheat stem rot.
Description
技术领域Technical field
本发明属于农药技术领域，具体涉及一种防治小麦茎基腐病的农药组合物。The invention belongs to the technical field of pesticides, and specifically relates to a pesticide composition for preventing and treating wheat stem rot.
背景技术Background technique
小麦茎基腐病是一种典型的土传病害，其病原比较复杂：包括黄色镰刀菌(Fusarium culmorum)、禾谷镰刀菌(Fusarium graminearum)和假禾谷镰刀菌(Fusariumpesudogramineaqum)等多种镰刀菌及其他一些病原菌，其中以假禾谷镰刀菌(F.pesudogramineaqum)为主的镰刀菌是中国黄淮麦区小麦茎基腐病的优势病原菌。Wheat stem rot is a typical soil-borne disease, and its pathogens are relatively complex: including Fusarium culmorum, Fusarium graminearum and Fusariumpesudogramineaqum, etc. and some other pathogenic bacteria, among which F. pesudogramineaqum is the dominant pathogen of wheat stem rot in the Huanghuai wheat region of China.
小麦茎基腐病从小麦分蘖期到成熟期均可发生，且在各小麦产区均有分布，严重影响小麦的产量。近年来，由于种植方式的转变及秸秆还田技术的大面积推广，病原菌在田间不断积累，使得我国小麦主产区的小麦茎基腐病频繁发生，且呈现出病害加重的趋势。Wheat stem rot can occur from the wheat tillering stage to the maturity stage, and is distributed in various wheat-producing areas, seriously affecting wheat yields. In recent years, due to changes in planting methods and the large-scale promotion of straw returning technology, pathogenic bacteria have continued to accumulate in the fields, causing frequent occurrence of wheat stem rot in my country's main wheat-producing areas, and showing a trend of worsening disease.
目前尚未选育出能够有效抵抗小麦茎基腐病的抗病小麦品种，在农业生产上对其仍以化学药剂防控为主，常用的化学药剂有：甲基硫菌灵、多菌灵、丙硫菌唑、戊唑醇、氟环唑和氰烯菌酯等。化学药剂具有见效快、防治效果好等优点，但长期施用单一化学药剂容易引起病原菌抗药性产生和发展，据报道小麦茎基腐病病原菌已对现有多种杀菌剂表现出不同程度的抗药性，使得对其的防治效果逐渐降低。而盲目大量农药剂量的方法，虽然短时间内提高防效，但这样会加重抗药性的发展。因此，开发新型化学药剂很有必要。At present, no disease-resistant wheat varieties have been bred that can effectively resist wheat stem rot. In agricultural production, chemical agents are still the main method of prevention and control. Commonly used chemical agents include: thiophanate-methyl, carbendazim, Prothioconazole, tebuconazole, epoxiconazole and cyanostrobin, etc. Chemical agents have the advantages of quick effects and good control effects, but long-term application of a single chemical agent can easily cause the emergence and development of resistance in pathogenic bacteria. It is reported that the pathogenic bacteria of wheat stem rot have shown varying degrees of resistance to a variety of existing fungicides. , causing its control effect to gradually decrease. The method of blindly dosing large amounts of pesticides can improve the control effect in a short period of time, but this will aggravate the development of resistance. Therefore, it is necessary to develop new chemical agents.
将不同农药品种进行复配筛选具有增效作用的组合，可以减少对环境的影响，同时还可以延缓病原菌抗药性的发展，是防治病害和开发新型药剂的经济有效手段。粉唑醇是先正达集团股份有限公司开发的三唑类甾醇脱甲基化抑制剂类杀菌剂。粉唑醇可有效抑制麦角甾醇的生物合成，并造成真菌细胞壁的破裂，具有广谱的抑菌活性和较强的内吸性，且对担子菌和子囊菌引起的许多真菌病害均具有良好的保护和治疗作用。Compounding different pesticide varieties to screen for synergistic combinations can reduce the impact on the environment and at the same time delay the development of drug resistance in pathogenic bacteria. It is an economical and effective means to prevent and treat diseases and develop new pharmaceuticals. Triazole is a triazole sterol demethylation inhibitor fungicide developed by Syngenta Group Co., Ltd. Fluconazole can effectively inhibit the biosynthesis of ergosterol and cause the rupture of fungal cell walls. It has broad-spectrum antibacterial activity and strong systemic properties, and has good effects on many fungal diseases caused by basidiomycetes and ascomycetes. Protective and therapeutic effects.
发明人通过室内试验发现粉唑醇与厚朴酚或蛇床子素进行二元复配时，对引起小麦茎基腐病的假禾谷镰刀菌具有增效作用，目前，还尚未见有这方面复配的相关报道。Through indoor experiments, the inventor found that when triconazole is combined with magnolol or osthole in a binary compound, it has a synergistic effect on Fusarium pseudograminearum, which causes wheat stem rot. At present, there is no such effect. Relevant reports on compounding.
公开于该背景技术部分的信息仅仅旨在增加对本发明的总体背景的理解，而不应当被视为承认或以任何形式暗示该信息构成已为本领域一般技术人员所公知的现有技术。The information disclosed in this Background section is merely intended to enhance an understanding of the general background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art that is already known to a person of ordinary skill in the art.
发明内容Contents of the invention
本发明的目的在于提供一种防治小麦茎基腐病的农药组合物，以解决长期施用单一化学药剂存在的问题。The object of the present invention is to provide a pesticide composition for preventing and treating wheat stem rot, so as to solve the problem of long-term application of a single chemical agent.
为实现上述目的，本发明提供了如下技术方案：In order to achieve the above objects, the present invention provides the following technical solutions:
一种防治小麦茎基腐病的农药组合物，所述农药组合物的有效成分由粉唑醇与厚朴酚或蛇床子素二元复配而成。A pesticide composition for preventing and treating wheat stem rot. The active ingredient of the pesticide composition is composed of a binary compound of flutriafol and honokiol or osthole.
更为具体的，所述粉唑醇与厚朴酚的质量比为1-9：9-1。More specifically, the mass ratio of the fluconazole and magnolol is 1-9:9-1.
更为具体的，所述粉唑醇与蛇床子素的质量比为1-17：27-1。More specifically, the mass ratio of fentriafol and osthole is 1-17:27-1.
本发明还提供了所述的农药组合物在防控小麦茎基腐病中的用途。The invention also provides the use of the pesticide composition in preventing and controlling wheat stem rot.
与现有技术相比，本发明具有如下有益效果：Compared with the prior art, the present invention has the following beneficial effects:
(1)本发明组合物中粉唑醇与厚朴酚或蛇床子素复配时，对引起小麦茎基腐病的假禾谷镰刀菌的共毒系数大于120，表现为协同增效作用，可以提高对小麦茎基腐病的防治效果，可以为开发防治小麦茎基腐病的新型药剂提供支持。(1) In the composition of the present invention, when triconazole is combined with honokiol or osthole, the co-toxicity coefficient against Fusarium pseudograminearum, which causes wheat stem rot, is greater than 120, showing a synergistic effect. It can improve the control effect of wheat stem rot and provide support for the development of new agents for the prevention and treatment of wheat stem rot.
(2)本发明组合物中粉唑醇与厚朴酚或蛇床子素复配时，可以有效延缓病原菌抗药性的发展，基于此可以减少农药施用剂量，降低防治成本和环境污染。(2) When compounded with magnolol or osthole in the composition of the present invention, flutriafol can effectively delay the development of drug resistance of pathogenic bacteria. Based on this, the dosage of pesticides can be reduced, and the cost of prevention and control and environmental pollution can be reduced.
具体实施方式Detailed ways
下面结合对本发明专利的技术方案进行清楚、完整的描述，显然，所描述的实施例是本发明的一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域所属的技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。The following is a clear and complete description of the technical solution of the patent of the present invention. Obviously, the described embodiments are some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts fall within the scope of protection of the present invention.
实施例：室内生物活性试验 Example : Indoor biological activity test
1.供试菌株1. Test strains
假禾谷镰刀菌(F.pesudogramineaqum)。采集染病麦株，在实验室内分离得到假禾谷镰刀菌，保存于PDA培养基上。F. pesudogramineaqum. Infected wheat strains were collected, F. pseudograminearum was isolated in the laboratory, and stored on PDA culture medium.
2.供试药剂2. Test reagents
95％粉唑醇原药、95％厚朴酚原药、98％蛇床子素原药，以上药剂均为市售。95% of the original drug of phenazole, 95% of the original drug of honokiol, and 98% of the original drug of osthole. The above drugs are all commercially available.
将供试药剂先用二甲基亚砜溶解，再用0.1％吐温-80水溶液稀释，配制成单剂母液，然后设置多组配比，各单剂母液和配比混剂均按等比方法设置5个质量浓度梯度。Dissolve the test reagent first with dimethyl sulfoxide, then dilute it with 0.1% Tween-80 aqueous solution, and prepare a single-dose mother liquor. Then set multiple groups of proportions. Each single-dose mother liquor and proportioned mixture are in equal proportions. The method sets up 5 mass concentration gradients.
3.试验方法(参考《NY/T 1156.2-2006农药室内生物测定准则杀菌剂第2部分：抑制病原真菌菌丝生长试验平皿法》)。3. Test method (refer to "NY/T 1156.2-2006 Pesticide Indoor Bioassay Guidelines for Fungicides Part 2: Inhibition of Pathogenic Fungal Mycelial Growth Test Plate Method").
将预先融化的45mL PDA培养基加入无菌锥形瓶中，冷却至50℃左右时，定量吸取5mL药液加入上述锥形瓶中，充分摇匀后倒入多个直径为9cm的培养皿中，每个培养皿10mL，冷却后形成相应浓度的含药平板；同时设不含药剂的处理作空白对照，每个质量浓度药液设置5个重复。Add 45 mL of pre-melted PDA culture medium into a sterile conical flask. When cooled to about 50°C, quantitatively add 5 mL of the drug solution into the above-mentioned conical flask. Shake well and pour into multiple petri dishes with a diameter of 9 cm. , each petri dish is 10 mL, and after cooling, a drug-containing plate of corresponding concentration is formed; at the same time, a treatment without the drug is set as a blank control, and 5 repetitions are set for each mass concentration of the drug solution.
用打孔器在供试菌株菌落边缘切取直径为5mm的菌饼，接种于含药平板和空白对照平板中央，盖上皿盖后置于30℃恒温箱培养72h，然后用十字交叉法测量菌落直径，计算不同处理对菌丝生长抑制率。Use a hole punch to cut a 5 mm diameter bacterial cake from the edge of the colony of the test strain, inoculate it into the center of the drug-containing plate and the blank control plate, cover the plate and place it in a 30°C incubator for 72 hours, and then measure the colonies using the cross-cross method. diameter, and calculate the inhibition rate of mycelium growth by different treatments.
4.数据分析：采用DPS软件进行数据统计分析，以杀菌剂浓度对数值为x，对应的菌丝生长抑制率几率值为y进行线性回归，得出毒力回归方程及药剂对靶标病菌的毒力EC50值，并根据孙云沛法计算共毒系数(CTC)。4. Data analysis: DPS software is used for statistical analysis of data. Using the logarithmic value of the fungicide concentration as The force EC 50 value was calculated, and the cotoxicity coefficient (CTC) was calculated according to the Sun Yunpei method.
5.测定结果5. Measurement results
根据计算的共毒系数(CTC)评价药剂的增效作用，CTC≤80为拮抗作用，80＜CTC＜120为相加作用，CTC≥120为增效作用，结果见表1-2。The synergistic effect of the agent is evaluated according to the calculated co-toxicity coefficient (CTC). CTC ≤ 80 is an antagonistic effect, 80 < CTC < 120 is an additive effect, and CTC ≥ 120 is a synergistic effect. The results are shown in Table 1-2.
表1粉唑醇和厚朴酚复配对假禾谷镰刀菌的室内生物活性Table 1 Indoor biological activity of flutriafol and honokiol compound against Fusarium pseudograminearum
由表1可知，粉唑醇和厚朴酚复配后，在质量比为1-9：9-1内对假禾谷镰刀菌的共毒系数均大于120，表现为增效作用。It can be seen from Table 1 that after compounding of flutriafol and honokiol, the co-toxicity coefficients against Fusarium pseudograminearum within the mass ratio of 1-9:9-1 are both greater than 120, showing a synergistic effect.
表2粉唑醇和蛇床子素复配对假禾谷镰刀菌的室内生物活性Table 2 Indoor biological activity of the combination of fentrileol and osthole against Fusarium pseudograminearum
由表2可知，粉唑醇和蛇床子素复配后，在质量比为1-17：27-1内对假禾谷镰刀菌的共毒系数均大于120，表现为增效作用。It can be seen from Table 2 that after the compounding of flutriafol and osthole, the co-toxicity coefficient against Fusarium pseudograminearum is greater than 120 within the mass ratio of 1-17:27-1, showing a synergistic effect.
综上所述，粉唑醇与厚朴酚或蛇床子素复配时，对引起小麦茎基腐病的假禾谷镰刀菌具有增效作用，可以提高小麦茎基腐病的防治效果，延缓病原菌抗药性的发展，基于此可以减少农药施用剂量，降低防治成本和环境污染。To sum up, when compounded with magnolol or osthole, flutriafol has a synergistic effect on Fusarium pseudograminearum, which causes wheat stem rot, and can improve the control effect of wheat stem rot and delay the development of wheat stem rot. The development of drug resistance of pathogenic bacteria can reduce pesticide application dosage, reduce prevention and control costs and environmental pollution.
前述对本发明的具体示例性实施方案的描述是为了说明和例证的目的。这些描述并非想将本发明限定为所公开的精确形式，并且很显然，根据上述教导，可以进行很多改变和变化。对示例性实施例进行选择和描述的目的在于解释本发明的特定原理及其实际应用，从而使得本领域的技术人员能够实现并利用本发明的各种不同的示例性实施方案以及各种不同的选择和改变。本发明的范围意在由权利要求书及其等同形式所限定。The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and illustration. These descriptions are not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teachings. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical applications, thereby enabling others skilled in the art to make and utilize various exemplary embodiments of the invention and various different applications. Choice and change. The scope of the invention is intended to be defined by the claims and their equivalents.
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