CN104273126B - A kind of bactericidal composition preventing and treating rice sheath blight disease - Google Patents

A kind of bactericidal composition preventing and treating rice sheath blight disease Download PDF

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Publication number
CN104273126B
CN104273126B CN201410512210.9A CN201410512210A CN104273126B CN 104273126 B CN104273126 B CN 104273126B CN 201410512210 A CN201410512210 A CN 201410512210A CN 104273126 B CN104273126 B CN 104273126B
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oligosaccharide
amino
flutolanil
sheath blight
rice sheath
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CN104273126A (en
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吴利民
陆宁海
霍云凤
杨蕊
郎剑锋
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Henan Institute of Science and Technology
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Henan Institute of Science and Technology
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Abstract

The present invention relates to a kind of bactericidal composition preventing and treating rice sheath blight disease, flutolanil is 20:1~1:5 with the mass ratio of amino-oligosaccharide.The described bactericidal composition containing flutolanil Yu amino-oligosaccharide, wherein effective active component content accounts for the 5~30% of gross weight, preferably 5~25%.Flutolanil used in the present invention belongs to two series bactericidal agents that the mechanism of action is different with amino-oligosaccharide, both will not produce antagonism at mutually mixture, in certain proportion, mixed synergies is notable, drug effect can be greatly improved, reduce Pesticide use amount and drug cost, the sheath blight fungus of the generation of jingganmycin is also had preferable prevention effect.

Description

A kind of bactericidal composition preventing and treating rice sheath blight disease
Technical field
The present invention relates to a kind of pesticide synergistic compositions, a kind of bactericidal composition preventing and treating rice sheath blight disease.
Background technology
Flutolanil, chemical name: 3'-isopropoxy-2-(trifluoromethyl) benzanilide, is Nihon Nihyaku Co., Ltd's exploitation New benzoyl for aniline fungicides, be the new medicament preventing and treating rice sheath blight disease at present, there is good drug efficacy, the preventing and treating time is long, Energy Oryza sativa L. improves the feature of setting percentage.
Amino-oligosaccharide, chemical name is: low poly-D glucosamine, and the one that this medicine microorganism belonging to genus metabolism is extracted has anti- The antibacterial of sick effect, the growth to some pathogenic bacteria has inhibitory action, and as affected fungus spore germination, induction hypha form occurs Variation, the intracellular biochemical reaction of mycelia changes, and induction plant produces the mechanism of disease resistance and mainly excites plant gene table Reach, produce and there are the chitinase of antibacterial action, glucanase, guarantor's element and PR albumen etc.;There is the gene table of suppression pathogenic bacteria simultaneously Reaching, make the Physiology and biochemistry of mycelia morph, growth is suppressed, simultaneously can also stimulating growth.Amino-oligosaccharide is used at present The preventing and treating of rice sheath blight disease has been reported.As Beiliu City, Guangxi plant protection unit, Guangxi District plant protection master station combine " the 5% amino widow delivered The sugar element water preparation study on prevention to late rice banded sclerotial blight " result shows: 5% amino-oligosaccharide AS can promote germination neat, Seedling growth, tiller and rice shoot root growth, reduce seedling-slowing stage and turn green the time, be 66.5% to rice sheath blight disease controlling effect, Effect of increasing production reaches 10.6%.
Banded sclerotial blight is one of three big diseases of Oryza sativa L., and in South Rice Region of China, harm is particularly acute, and this disease makes Oryza sativa L. not ear, or The hollow kernels of heading is more, and mass of 1000 kernel reduces, and the general underproduction 5%~10%, seriously up to 50%~70%.For many years, rice sheath blight disease is long Phase relies on the single medicament such as jingganmycin, thiophanate-methyl to prevent and treat, and causes pathogenic bacteria to produce bigger Drug resistance.Base of the present invention Characteristic in flutolanil and amino-oligosaccharide further study show that, the mixed agent of flutolanil and amino-oligosaccharide is used for rice banded sclerotial blight Sick preventing and treating, has obvious potentiation, can reduce Pesticide use amount, have preventive effect higher, the advantage that mu is the lowest.
And there is presently no and flutolanil, amino-oligosaccharide are carried out mixture potentiation, for the relevant report of rice sheath blight disease controlling.
Summary of the invention
The technical problem to be solved is, it is provided that it is notable that one prevents and treats rice sheath blight disease potentiation, preventive effect is higher, Cost is lower and can prevent disease from developing immunity to drugs or the pesticide synergistic compositions of delaying drug resistance speed.
The present invention solves above-mentioned technical problem and is adopted the technical scheme that: a kind of bactericidal composition preventing and treating rice sheath blight disease, fluorine Amide is 20:1~1:5 with the mass ratio of amino-oligosaccharide.
The described bactericidal composition preventing and treating rice sheath blight disease, wherein effective active component content accounts for the 5~30% of gross weight, preferably It is 5~25%.
The described bactericidal composition preventing and treating rice sheath blight disease, according to method known in those skilled in the art, adds corresponding additive And solvent, filler, make any dosage form agriculturally allowed, the most such as: water dispersible granules, suspending agent, wettable powder.
The auxiliary agent used in the bactericidal composition of the present invention includes dispersant, wetting agent, antifreeze, defoamer, thickening agent, collapses Solve agent, emulsifying agent, stabilizer, binding agent or other be of value to the material that effective ingredient is stable in the formulation and drug effect plays, all It is conventional in pesticidal preparations or the various compositions of permission use.
The present invention has the following advantages and belongs to, with significant technique effect, flutolanil and amino-oligosaccharide, two classes that the mechanism of action is different Antibacterial, both mutual mixture will not produce antagonism, and in certain proportion, mixed synergies is notable, it is possible to significantly carry High-drug-effect, reduces Pesticide use amount and drug cost, the sheath blight fungus of the generation of jingganmycin is also had preferable prevention effect.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme and technique effect are described further, of the present invention hundred Proportion by subtraction is all weight percentage.
Embodiments of the invention use the method that Toxicity Determination and field test combine.
Implementation of an application example one: flutolanil is compounding with amino-oligosaccharide to rice sheath blight disease Toxicity Determination
After prerun determines each medicament effective inhibition concentration scope, each medicament is set 5 dosage respectively and processes by active constituent content, If clear water compares.Carry out with reference to " farm-chemical indoor determination test rule antibacterial ", use mycelial growth rate method to measure medicine The agent virulence to rice sheath blight disease.Measure colony diameter by decussation method after 72h, calculate each process net growth, mycelial growth Suppression ratio.Mycelial growth inhibition rate is converted into probit value (y), and liquor strength (μ g/mL) is converted into logarithm value (x), with Little square law tries to achieve virulence regression equation (y=a+bx), and thus calculates the EC of every kind of medicament50Value.With being total to of the abundant method of Sun Yun The potentiation of pharmacy mix evaluated by poison Y-factor method Y (CTC), i.e. CTC≤80 are antagonism, and 80 < CTC < 120 is phase Adding effect, CTC >=120 are potentiation.
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100
B in the percentage composition+B medicament toxicity index × mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture Percentage composition
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100
Result of the test is as shown in table 1:
Table 1 flutolanil, amino-oligosaccharide and the compounding toxicity test analysis of results table to rice sheath blight disease thereof
Reagent agent Proportioning EC50(mg/L) ATI TTI Co-toxicity coefficient
Flutolanil / 1.12 100 / /
Amino-oligosaccharide / 3.02 37.1 / /
Flutolanil: amino-oligosaccharide 20:1 0.87 128.7 97.0 132.7
Flutolanil: amino-oligosaccharide 10:1 0.82 136.6 94.3 144.9
Flutolanil: amino-oligosaccharide 5:1 0.81 138.3 89.5 154.5
Flutolanil: amino-oligosaccharide 1:1 0.92 121.7 68.5 177.6
Flutolanil: amino-oligosaccharide 2:5 0.98 114.3 55.1 207.4
Flutolanil: amino-oligosaccharide 1:5 1.64 68.3 47.6 143.5
Flutolanil: amino-oligosaccharide 1:7 2.21 50.7 45.0 112.6
From the statistical analysis of table 1, flutolanil, the amino-oligosaccharide EC to rice sheath blight disease50Be respectively 1.12mg/L and 3.02mg/L.The virulence of amino-oligosaccharide is less than the virulence of flutolanil.Flutolanil and amino-oligosaccharide are in 20:1~1:5 scope Interior mixture all shows obvious potentiation.When the mass ratio of flutolanil Yu amino-oligosaccharide is 1:7, co-toxicity coefficient is 112.6, show as summation action.
The implementation of an application example two bactericidal composition containing flutolanil and amino-oligosaccharide prevents and treats the field control effectiveness test of rice sheath blight disease
This experimental establishment is in Wuming County doube bridge town, Nanning, and test period is on October 20th, 2013, with flutolanil and amino The former medicine of oligosaccharide according to method known in those skilled in the art, adds corresponding additive in the range of mass ratio is 20:1~1:5 And solvent, filler, make the mixture preparation of flutolanil amino-oligosaccharide as test medicine, with 20% flutolanil wettable powder Agent (commercial), 5% amino-oligosaccharide water preparation (commercial) use identical application method, water consumption as comparison medicament, each community It it is 45 kgs/acre.Investigating the rice sheath blight disease state of an illness before medicine, in the dispenser for the first time of state of an illness initial stage, every 7 days, dispenser once, was executed altogether again Medicine 2 times.Within after last dispenser 7 days, 14 days, investigate rice sheath blight disease disease index respectively and calculate preventive effect.
Experimental result is as shown in table 2:
The table 2 bactericidal composition containing flutolanil and amino-oligosaccharide prevents and treats the field control effectiveness test of rice sheath blight disease
As can be seen from Table 2, the mixture preparation of flutolanil and amino-oligosaccharide is for the preventing and treating of rice sheath blight disease, and effect is substantially better than Comparison medicament, reduces Pesticide use amount, effectively reduces mu originally, can effectively control the harm of rice sheath blight disease, tests medication In the range of the growth of rice seedling is not had harmful effect.

Claims (3)

1. the bactericidal composition preventing and treating rice sheath blight disease, it is characterised in that effective ingredient is by flutolanil and amino-oligosaccharide group Becoming, flutolanil is 10:1~1:5 with the mass ratio of amino-oligosaccharide.
The bactericidal composition preventing and treating rice sheath blight disease the most according to claim 1, it is characterised in that described flutolanil and ammonia The content of base oligosaccharide accounts for the 5~30% of gross weight.
The bactericidal composition preventing and treating rice sheath blight disease the most according to claim 1, it is characterised in that described compositions is water Dispersible granule, suspending agent or wettable powder.
CN201410512210.9A 2014-09-29 2014-09-29 A kind of bactericidal composition preventing and treating rice sheath blight disease Expired - Fee Related CN104273126B (en)

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CN106818755A (en) * 2017-04-14 2017-06-13 广西南宁益土生物科技有限责任公司 A kind of bactericidal composition for preventing and treating rice sheath blight disease

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102276356A (en) * 2011-07-11 2011-12-14 广西田园生化股份有限公司 Stable controlled release particle pesticide-containing fertilizer
CN103314992A (en) * 2013-07-02 2013-09-25 海南正业中农高科股份有限公司 Insecticide acaricide taking oligose and macrolides composition as synergist and application of insecticide acaricide
CN103392737A (en) * 2013-07-26 2013-11-20 海南正业中农高科股份有限公司 Agricultural composition taking oligosaccharide and sulphur as synergistic components

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102276356A (en) * 2011-07-11 2011-12-14 广西田园生化股份有限公司 Stable controlled release particle pesticide-containing fertilizer
CN103314992A (en) * 2013-07-02 2013-09-25 海南正业中农高科股份有限公司 Insecticide acaricide taking oligose and macrolides composition as synergist and application of insecticide acaricide
CN103392737A (en) * 2013-07-26 2013-11-20 海南正业中农高科股份有限公司 Agricultural composition taking oligosaccharide and sulphur as synergistic components

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