CN116473062A - Sterilization composition for preventing and treating passion fruit stem basal rot - Google Patents

Sterilization composition for preventing and treating passion fruit stem basal rot Download PDF

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Publication number
CN116473062A
CN116473062A CN202310463753.5A CN202310463753A CN116473062A CN 116473062 A CN116473062 A CN 116473062A CN 202310463753 A CN202310463753 A CN 202310463753A CN 116473062 A CN116473062 A CN 116473062A
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passion fruit
fruit stem
rot
preventing
fludioxonil
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CN202310463753.5A
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CN116473062B (en
Inventor
杨祥燕
龙薇平
蔡元保
邱文武
杨柳
董龙
蔡昭艳
黄永才
陈格
桂杰
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Guangxi Subtropical Crops Research Institute
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Guangxi Subtropical Crops Research Institute
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/50Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a sterilizing composition for preventing and treating passion fruit stem basal rot. The active ingredients of the bactericidal composition are compounded by the triflumamid and fludioxonil or trifloxystrobin. The bactericidal composition provided by the invention has synergistic effect on inhibiting the growth of fusarium mycelia of passion fruit stem rot pathogen, can improve the control effect on passion fruit stem rot, is beneficial to reducing the application amount of active ingredients, and reduces the control cost and environmental pressure.

Description

Sterilization composition for preventing and treating passion fruit stem basal rot
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a sterilizing composition for preventing and treating passion fruit stem basal rot.
Background
Passion fruit is also called passion fruit and passion fruit, which is a tropical fruit and has the American name of "king of beverage". The stem basal rot is one of the main diseases of passion fruit, and mostly occurs at the stem basal part of the plant, which is 5cm to 15cm away from the ground, after the passion fruit is infected at the seedling stage, the stem basal part is dark brown speck, the stem basal layer is gradually softened and then decayed, brown specks are generated, the leaves of the plant are withered, yellow and fall off, the branches and tendrils are slowly withered and even the plant is withered, and the plant is a destructive disease in the passion fruit planting process. There are various pathogens causing passion fruit stem rot, including fusarium, fusarium oxysporum, phytophthora, trichoderma, and new trichoderma species.
In the passion fruit production process, medicaments such as mancozeb, carbendazim, prochloraz, difenoconazole, tebuconazole, fludioxonil, thiophanate methyl and the like can be used for preventing and treating passion fruit stem rot. However, with the long-term use of these agents, pathogenic bacteria have developed a degree of resistance to them, resulting in a decrease in control effect. The application dosage of the medicament is blindly increased, the control effect can be improved only in a short time, and meanwhile, the negative effects of accelerating the development of drug resistance, increasing pesticide residues and the like are also present.
The trifluoropyridinamine is a novel SDHI bactericide developed in 2013 of Zhengda company, and has the English name: cycloobutrifluram, molecular formula: c (C) 17 H 13 Cl 2 F 3 N 2 O has the following structural formula:
as a member of the SDHI class of bactericides, trifluoropyridinamine is also an inhibitor of pathogenic bacteria respiration, inhibiting mitochondrial function by interfering with tricarboxylic acid circulation on respiratory electron transfer chain complex II, preventing it from generating energy, inhibiting pathogenic bacteria growth, and ultimately leading to its death. In addition, the trifluoropyridinamine can also paralyze nematode larvae through interference of respiration, thereby leading to energy exhaustion in cells and inhibiting egg hatching. The trifluoropyridinamine has broad spectrum and high efficiency, can provide durable protection effect for crops, and prevents the crops from being damaged by nematodes and soil-borne diseases which are difficult to find but extremely damaging, especially fusarium.
Although trifluoropyridinamine has the advantages, the action mechanism of the trifluoropyridinamine on pathogenic bacteria is single, and if the trifluoropyridinamine is singly used for a long time, the higher drug resistance risk exists. The compounding of different medicaments is an effective and convenient way for developing a novel medicament, improving the target control effect and delaying the generation of drug resistance. At present, no report on the compounding of the triflumamid and fludioxonil or trifloxystrobin is seen.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and controlling passion fruit stem basal rot, which aims to solve the problem that pathogenic bacteria have generated a certain degree of drug resistance to the passion fruit stem basal rot with long-term use of a medicament, so that the preventing and controlling effect is reduced. The application dosage of the medicament is blindly increased, the control effect can be improved only in a short time, and the problems of accelerating the development of drug resistance, increasing pesticide residues and other negative effects are also solved.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the active ingredients of the bactericidal composition are compounded by the triflumamid and fludioxonil or trifloxystrobin.
More specifically, the mass ratio of the trifluoropyridinamine to fludioxonil is 1-7:15-1.
More specifically, the mass ratio of the triflumamid to the orysastrobin is 1-20:20-1.
The invention also provides application of the bactericidal composition in preventing and treating passion fruit stem basal rot.
More specifically, the passion fruit stem basal rot is caused by fusarium putrescens.
Compared with the prior art, the invention has the following beneficial effects:
(1) The bactericidal composition provided by the invention has synergistic effect on inhibiting the growth of fusarium mycelia of passion fruit stem rot pathogen, can improve the control effect on passion fruit stem rot, is beneficial to reducing the application amount of active ingredients, and reduces the control cost and environmental pressure.
(2) The bactericidal composition can delay the generation and development of drug resistance of pathogenic bacteria and reduce the risk of drug resistance by using a single effective component.
Detailed Description
The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: screening of passion fruit stem rot disease control medicament
1. Test strain
Collecting disease samples from the planting land of passion fruit, separating and purifying pathogenic bacteria in a laboratory by adopting a tissue separation method, identifying the passion fruit stem base rot pathogenic bacteria as Fusarium solani, and storing the Fusarium solani on a PDA culture medium.
2. Test agent
86% of trifluopicolamine (Switzerland is reaching crop protection Co., ltd.), 98% of fludioxonil (Shanghai He medical Co., ltd.), 98% of orysastrobin (Hubei Shuangalling Co., ltd.).
3. Test methods (cf. Section 2. Methods for test criteria for biological tests in pesticide laboratory: petri dish method for inhibition of growth of mycelium of pathogenic fungi).
Dissolving the test agent with dimethyl sulfoxide, diluting with 0.1% Tween-80 aqueous solution to obtain single-agent mother solution, setting multiple groups of ratios, and setting 5 mass concentration gradients for each single-agent mother solution and ratio mixture according to an equal ratio method. Adding 9mL of pre-melted PDA culture medium into a sterile conical flask, sequentially quantitatively sucking 1mL of liquid medicine from low concentration to high concentration, respectively adding into the conical flask, shaking uniformly, pouring into a culture dish with the diameter of 9cm, and preparing a medicine-containing flat plate with corresponding concentration; treatments without agent were also included as blank, and 9 replicates were set for each treatment. Cutting bacterial cakes with the diameter of 5mm from the colony edge of the strain to be tested by using a puncher, inoculating the bacterial cakes to the centers of a medicine-containing flat plate and a blank control flat plate, covering a dish cover, placing the dish cover in a constant temperature oven at 28 ℃ for culturing for 7d, measuring the colony diameter by using a crisscross method, and calculating the hypha growth inhibition rate of different treatments.
4. Data analysis: performing data statistical analysis by DPS software, and linearly regressing with the value of the disinfectant concentration logarithmic value x and the value of the corresponding hypha growth inhibition rate logarithmic value y to obtain a virulence regression equation and the virulence EC of the medicament on the target pathogen 50 Values and co-toxicity coefficients (CTCs) were calculated according to the grand cloud Pei method.
5. Measurement results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-2.
TABLE 1 determination of indoor biological Activity of Fusarium putrescens by the combination of Triflupicoline and fludioxonil
Medicament name and ratio EC50(mg/L) ATI TTI CTC
Trifluoro pyridine amine 0.3596 100.0000 -- --
Fludioxonil 0.9495 37.8726 -- --
Trifluoropyridinamine 1: fludioxonil 15 0.3625 99.2000 41.7555 237.5733
Trifluoropyridinamine 1: fludioxonil 7 0.2016 178.3730 45.6385 390.8390
Trifluoropyridinamine 1: fludioxonil 5 0.6127 58.6910 48.2271 121.6971
Trifluoropyridinamine 1: fludioxonil 3 0.4561 78.8424 53.4044 147.6326
Trifluoropyridinamine 1: fludioxonil 1 0.3924 91.6412 68.9363 132.9361
Trifluoropyridinamine 3: fludioxonil 1 0.2455 146.4766 84.4681 173.4104
Trifluoropyridinamine 5: fludioxonil 1 0.3111 115.5898 89.6454 128.9411
Trifluoropyridinamine 7: fludioxonil 1 0.2357 152.5668 92.2341 165.4127
As can be seen from table 1, after the combination of the trifluoropyridinamine and fludioxonil, the mass ratio is 1-7: the co-toxicity coefficient of the fusarium solani to fusarium solani within the range of 15-1 is more than 120, and the synergistic effect is shown.
TABLE 2 determination of indoor biological Activity of Fusarium putrescens by the combination of triflumicarb-isopropyl and trifloxystrobin
As shown in Table 2, after the triflumuron and the orysastrobin are compounded, the mass ratio is 1-20: the co-toxicity coefficient of the fusarium solani to fusarium solani within the range of 20-1 is more than 120, and the synergistic effect is shown.
In conclusion, the trifluoropyridinamine and fludioxonil or the orysastrobin have synergistic effect on inhibiting the growth of fusarium mycelia of passion fruit stem rot pathogen, so that the control effect on passion fruit stem rot can be improved, the application amount of active ingredients can be reduced, and the control cost and the environmental pressure can be reduced.

Claims (5)

1. The sterilizing composition for preventing and treating passion fruit stem rot is characterized in that the effective components of the sterilizing composition are compounded by trifluoropyridylamine and fludioxonil or trifloxystrobin.
2. The bactericidal composition of claim 1, wherein the mass ratio of the trifluoropicoline amine to fludioxonil is 1-7:15-1.
3. The bactericidal composition of claim 1, wherein the mass ratio of the triflumamid to the orysastrobin is 1-20:20-1.
4. Use of a bactericidal composition according to any of claims 1-3 for controlling passion fruit stem rot.
5. The use according to claim 4, characterized in that said passion fruit stem base rot is caused by fusarium solani.
CN202310463753.5A 2023-04-26 Sterilization composition for preventing and treating passion fruit stem basal rot Active CN116473062B (en)

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Application Number Priority Date Filing Date Title
CN202310463753.5A CN116473062B (en) 2023-04-26 Sterilization composition for preventing and treating passion fruit stem basal rot

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Application Number Priority Date Filing Date Title
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CN116473062A true CN116473062A (en) 2023-07-25
CN116473062B CN116473062B (en) 2024-09-27

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111990405A (en) * 2020-09-15 2020-11-27 美丰农业科技(上海)有限公司 Nematicidal agricultural composition containing fluopyram and Cyclobutrifluram
CN114176087A (en) * 2021-12-20 2022-03-15 青岛滕润翔检测评价有限公司 Pesticide composition and application thereof
CN118019451A (en) * 2021-10-29 2024-05-10 先正达农作物保护股份公司 Fungicidal compositions comprising fludioxonil

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111990405A (en) * 2020-09-15 2020-11-27 美丰农业科技(上海)有限公司 Nematicidal agricultural composition containing fluopyram and Cyclobutrifluram
CN118019451A (en) * 2021-10-29 2024-05-10 先正达农作物保护股份公司 Fungicidal compositions comprising fludioxonil
CN114176087A (en) * 2021-12-20 2022-03-15 青岛滕润翔检测评价有限公司 Pesticide composition and application thereof

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