CN117652528A - Biological pesticide for preventing and treating oculopsis - Google Patents
Biological pesticide for preventing and treating oculopsis Download PDFInfo
- Publication number
- CN117652528A CN117652528A CN202311652549.4A CN202311652549A CN117652528A CN 117652528 A CN117652528 A CN 117652528A CN 202311652549 A CN202311652549 A CN 202311652549A CN 117652528 A CN117652528 A CN 117652528A
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- Prior art keywords
- bacillus sphaericus
- preventing
- oculopsis
- biological pesticide
- controlling
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000575 pesticide Substances 0.000 title claims abstract description 18
- 230000003405 preventing effect Effects 0.000 title claims abstract description 16
- 241000193386 Lysinibacillus sphaericus Species 0.000 claims abstract description 33
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000003814 drug Substances 0.000 claims abstract description 11
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 claims abstract description 10
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000238631 Hexapoda Species 0.000 claims abstract description 7
- 229940079593 drug Drugs 0.000 claims abstract description 7
- 239000004593 Epoxy Substances 0.000 claims abstract description 6
- 241000255925 Diptera Species 0.000 claims description 7
- 239000005906 Imidacloprid Substances 0.000 claims description 6
- 230000002159 abnormal effect Effects 0.000 claims description 6
- 229940056881 imidacloprid Drugs 0.000 claims description 6
- 230000003551 muscarinic effect Effects 0.000 claims description 4
- 240000001462 Pleurotus ostreatus Species 0.000 claims description 3
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims description 3
- YWTYJOPNNQFBPC-UHFFFAOYSA-N imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- 241000257229 Musca <genus> Species 0.000 claims 2
- 244000068697 Vitis rotundifolia Species 0.000 claims 2
- 235000006359 Vitis rotundifolia var rotundifolia Nutrition 0.000 claims 2
- 235000001270 Allium sibiricum Nutrition 0.000 claims 1
- 241001494113 Bradysia Species 0.000 claims 1
- 244000184734 Pyrus japonica Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 23
- 230000001276 controlling effect Effects 0.000 abstract description 9
- 241000256113 Culicidae Species 0.000 abstract description 6
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract description 5
- 238000013329 compounding Methods 0.000 abstract description 5
- 241000993291 Armillariella Species 0.000 abstract description 4
- 239000000447 pesticide residue Substances 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 239000005923 Pirimicarb Substances 0.000 abstract description 2
- 230000002401 inhibitory effect Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 8
- 241000607479 Yersinia pestis Species 0.000 description 7
- 241001534815 Hypsizygus marmoreus Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 230000001094 effect on targets Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000216654 Armillaria Species 0.000 description 1
- 241000613693 Bradysia odoriphaga Species 0.000 description 1
- 244000252132 Pleurotus eryngii Species 0.000 description 1
- 235000001681 Pleurotus eryngii Nutrition 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- -1 guanidine pentose Chemical class 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Insects & Arthropods (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of biological pesticides, and in particular relates to a biological pesticide for preventing and treating oculopsis. A biological pesticide for preventing and treating Armillariella mosquitos is prepared by compounding Bacillus sphaericus with epoxy-cloprid or Wu-pirimicarb; wherein the Bacillus sphaericus is 200ITU/mg Bacillus sphaericus parent drug. The biological pesticide for preventing and controlling the oculopsis is formed by compounding bacillus sphaericus and epoxy insect or pentosan and guanidine, has a synergistic effect on the oculopsis in a certain mass ratio range, can improve the preventing and controlling effect on the edible fungus oculopsis, is beneficial to reducing the dosage of the preventing and controlling agent, avoids the excessive pesticide residue of the edible fungus, and has important significance on the green sustainable development of edible fungus planting industry. In addition, the biological pesticide of the invention has the concentration EC in inhibiting the growth of edible fungus hypha 50 Higher, will not be to the domestic fungusThe growth is affected, and the edible fungi are safe.
Description
Technical Field
The invention belongs to the technical field of biological pesticides, and in particular relates to a biological pesticide for preventing and treating oculopsis.
Background
The larva of the insect belonging to the Armillariella mosquitidae bites the mycelium, fruiting body and young mushroom bud or borer stem of the edible fungi, and the feeding movement of the larva and the adult can spread pathogenic bacteria, so that the edible fungi are polluted by the pathogenic bacteria, and the production of the edible fungi is influenced. At present, the eumythiidae pests which are harmful to edible fungi mainly comprise oyster mushroom, hypsizygus marmoreus, heterotypic Hypsizygus marmoreus and slow-release Hypsizygus marmoreus.
The method for preventing and controlling the edible fungus and the oculopsis mosquito has a plurality of defects, but the chemical prevention and control method is convenient to apply and takes effect quickly, and is the main method for preventing and controlling the edible fungus and the oculopsis mosquito at present. But as the resistance of the target pest to the chemical agent is accumulated, the control effect is reduced. The control effect on target pests can be improved by increasing the usage amount of chemical agents, but the edible fungus pesticide residues are easy to exceed the standard.
Different insecticidal active ingredients are compounded, and a compound formula with a synergistic effect is screened, so that the control effect on target pests can be improved, and the method is an effective and convenient means for solving the problems and comprehensively controlling drug-resistant pests. At present, no report related to the compounding of bacillus sphaericus with epoxy-imidacloprid or pentosan-imidacloprid is seen.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a biological pesticide for preventing and treating the oculopsis, which can effectively prevent and treat the oculopsis on edible fungi, does not influence the growth of the edible fungi, and is safe to the edible fungi.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a biological pesticide for preventing and treating Armillariella mosquitos is prepared by compounding Bacillus sphaericus with epoxy-cloprid or Wu-pirimicarb; wherein the Bacillus sphaericus is 200ITU/mg Bacillus sphaericus parent drug.
Preferably, the mass ratio of the bacillus sphaericus to the epoxy-insect-ine is 1-9:19-1.
Preferably, the mass ratio of the bacillus sphaericus to the guanidine pentose is 1-20:20-1.
Preferably, the muscarinic mosquitoes include the oyster mushroom, the heteromythic mosquitos and the bradysia odoriphaga.
Compared with the prior art, the invention has the following beneficial effects:
(1) The biological pesticide for preventing and controlling the oculopsis is formed by compounding bacillus sphaericus and epoxy insect or pentosan and guanidine, has a synergistic effect on the oculopsis in a certain mass ratio range, can improve the preventing and controlling effect on the edible fungus oculopsis, is beneficial to reducing the dosage of the preventing and controlling agent, avoids the excessive pesticide residue of the edible fungus, and has important significance on the green sustainable development of edible fungus planting industry.
(2) The biological pesticide of the invention has the concentration EC for inhibiting the growth of edible fungus hypha 50 Higher, can not have influence on the growth of edible fungi, and is safe for the edible fungi.
Detailed Description
The following description of the embodiments of the present invention will be apparent from the description of the embodiments of the present invention, which is provided in part, but not in whole. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Example 1: indoor biological activity test of compound medicament on target
1. Test pest
Selecting normal abnormal euhedronella eugenic male and female adults, continuously feeding the euhedronella eugenic male and female adults with a proper amount of clear water and fresh and clean pleurotus eryngii for 10 generations in a laboratory, the 3-year old heteroclite mosquito larvae were selected as test subjects.
2. Test agent
200ITU/mg Bacillus sphaericus parent drug (Jiangsu Yangzhou green source biochemical Co., ltd.), 95% epoxy-worm-line parent drug (Sichuan and bang biotechnology Co., ltd.), 96% Wu-worm guanidine parent drug (He-Fei-xing Chemicals Co., ltd.).
Sterile water for the bacillus sphaericus parent medicine is prepared into single-dose mother liquor with the concentration of 10000 mg/L; after the original imidacloprid and the original pentosan guanidine are dissolved in dimethyl sulfoxide, the mixture is diluted into single-dose mother solution with the concentration of 10000mg/L by using a tween-80 aqueous solution with the mass fraction of 0.1 percent. Setting a plurality of groups of proportions, and setting 5 mass concentration gradients for each single-dose mother solution and the proportion mixture according to an equal proportion method for standby. The preparation can be prepared for use at present.
3. Test method
Adopts liquid medicine soaking feeding method. Soaking the side ear pieces of radix Apii with the same size in the medicinal liquid for 10min, taking out, sucking excessive medicinal liquid with filter paper, placing in a culture dish with filter paper laid at the bottom, keeping the filter paper wet with the same medicinal liquid, and picking into each dish for 20 heads of tested pests. Soaking and moisturizing with 0.1% Tween-80 aqueous solution as blank, repeating each treatment for 4 times, observing death condition of larvae after 72 hr in a constant temperature incubator with a temperature of 25deg.C and a relative humidity of 75%, calculating corrected mortality of each treatment, performing regression analysis on the concentration log value and corrected mortality probability value of each treatment agent, and calculating LC of each treatment agent 50 。
In the above formula: p- -mortality in units of; k- -number of dead insects; n- -total number of insects treated.
In the above formula: p (P) 1 -correct mortality in units of; p (P) t -mortality rate in units of treatment; p (P) 0 Blank mortality in%.
4. Evaluation of pharmaceutical Agents
Co-toxicity coefficients (CTC values) of the blends were calculated according to the grand cloud Pei method and the criteria were divided according to the combined effect: the co-toxicity coefficient (CTC) is more than or equal to 120, and the synergistic effect is shown; the cotoxicity coefficient (CTC) is less than or equal to 80 and shows antagonism; 80< co-toxicity coefficient (CTC) <120 shows additive effect. The experimental results are shown in tables 1-2.
Table 1 indoor biological Activity determination of Bacillus sphaericus and Epoxicillin Complex to Hypsizygus marmoreus
| Medicament name and ratio | LC50(mg/L) | ATI | TTI | CTC |
| Bacillus sphaericus | 45.9032 | 100.0000 | -- | -- |
| Epoxy insect-like compound | 6.5561 | 700.1602 | -- | -- |
| Bacillus sphaericus 1: epoxine 19 | 5.2107 | 880.9411 | 670.1521 | 131.4539 |
| Bacillus sphaericus 1: epoxine 15 | 5.6138 | 817.6850 | 662.6501 | 123.3962 |
| Bacillus sphaericus 1: epoxy insect 9 | 4.2847 | 1071.3282 | 640.1441 | 167.3573 |
| Bacillus sphaericus 1: epoxine 7 | 6.0191 | 762.6256 | 625.1401 | 121.9927 |
| Bacillus sphaericus 1: epoxy insect-line 3 | 6.9061 | 664.6762 | 550.1201 | 120.8238 |
| Bacillus sphaericus 1: epoxine 1 | 7.8001 | 588.4950 | 400.0801 | 147.0943 |
| Bacillus sphaericus 3: epoxine 1 | 11.6831 | 392.9026 | 250.0400 | 157.1359 |
| Bacillus sphaericus 7: epoxine 1 | 15.2780 | 300.4529 | 175.0200 | 171.6678 |
| Bacillus sphaericus 9: epoxine 1 | 18.4724 | 248.4961 | 160.0160 | 155.2945 |
As shown in Table 1, the combination of Bacillus sphaericus and imidacloprid shows synergistic effect on killing abnormal Armillaria mosquitos, and can improve the control effect on abnormal Armillariella mosquitos.
Table 2 indoor bioactivity assay of bacillus sphaericus and guanidine pentadactyle complex on heterotypic muscarinic mosquitoes
As shown in Table 2, the combination of Bacillus sphaericus and guanidine N.pentosaceus has synergistic effect on killing abnormal Hypsizygus marmoreus, and can improve the control effect on abnormal Hypsizygus marmoreus.
In conclusion, the bacillus sphaericus and the epoxy-imidacloprid or the pentadactyla guanidine are compounded, so that the prevention and control effect on the abnormal-shape oculopsis mosquitoes can be improved, the pesticide use amount can be reduced, the excessive pesticide residue of the edible fungi can be avoided, and the method has important significance for the green sustainable development of edible fungus planting industry.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (4)
1. A biological pesticide for preventing and controlling the oculopsis is characterized in that the active ingredient is compounded by bacillus sphaericus and epoxy insect line or penyfish guanidine; wherein the Bacillus sphaericus is 200ITU/mg Bacillus sphaericus parent drug.
2. The biological pesticide for controlling the muscarinic mosquitoes according to claim 1, wherein the mass ratio of bacillus sphaericus to imidacloprid is 1-9:19-1.
3. The biological pesticide for controlling the muscarinic mosquitoes according to claim 1, wherein the mass ratio of bacillus sphaericus to guanidine pentadactyl is 1-20:20-1.
4. The biological pesticide for controlling the musca volitantes according to claim 1, wherein the musca volitantes comprises oyster mushroom muscadine, abnormal muscadine and chive bradysia japonica.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311652549.4A CN117652528A (en) | 2023-12-05 | 2023-12-05 | Biological pesticide for preventing and treating oculopsis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311652549.4A CN117652528A (en) | 2023-12-05 | 2023-12-05 | Biological pesticide for preventing and treating oculopsis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117652528A true CN117652528A (en) | 2024-03-08 |
Family
ID=90083967
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311652549.4A Pending CN117652528A (en) | 2023-12-05 | 2023-12-05 | Biological pesticide for preventing and treating oculopsis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117652528A (en) |
-
2023
- 2023-12-05 CN CN202311652549.4A patent/CN117652528A/en active Pending
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