CN116806847B - Active microbial agent - Google Patents
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- CN116806847B CN116806847B CN202310785034.5A CN202310785034A CN116806847B CN 116806847 B CN116806847 B CN 116806847B CN 202310785034 A CN202310785034 A CN 202310785034A CN 116806847 B CN116806847 B CN 116806847B
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- bacillus cereus
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 17
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 32
- AZSNMRSAGSSBNP-UHFFFAOYSA-N 22,23-dihydroavermectin B1a Natural products C1CC(C)C(C(C)CC)OC21OC(CC=C(C)C(OC1OC(C)C(OC3OC(C)C(O)C(OC)C3)C(OC)C1)C(C)C=CC=C1C3(C(C(=O)O4)C=C(C)C(O)C3OC1)O)CC4C2 AZSNMRSAGSSBNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- SPBDXSGPUHCETR-JFUDTMANSA-N 8883yp2r6d Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O[C@@H]([C@@H](C)CC4)C(C)C)O3)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1C[C@H](C)[C@@H]([C@@H](C)CC)O[C@@]21O[C@H](C\C=C(C)\[C@@H](O[C@@H]1O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C1)[C@@H](C)\C=C\C=C/1[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\1)O)C[C@H]4C2 SPBDXSGPUHCETR-JFUDTMANSA-N 0.000 claims abstract description 12
- 229960002418 ivermectin Drugs 0.000 claims abstract description 12
- 239000004480 active ingredient Substances 0.000 claims abstract description 4
- 150000001875 compounds Chemical class 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 abstract description 23
- 241000607479 Yersinia pestis Species 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 12
- 230000002195 synergetic effect Effects 0.000 abstract description 9
- 239000000575 pesticide Substances 0.000 abstract description 8
- 206010059866 Drug resistance Diseases 0.000 abstract description 6
- 238000013329 compounding Methods 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 239000000447 pesticide residue Substances 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 241000498254 Heterodera glycines Species 0.000 description 11
- 239000003814 drug Substances 0.000 description 11
- 239000005783 Fluopyram Substances 0.000 description 10
- 241000244206 Nematoda Species 0.000 description 10
- 241001465752 Purpureocillium lilacinum Species 0.000 description 10
- KVDJTXBXMWJJEF-UHFFFAOYSA-N fluopyram Chemical compound ClC1=CC(C(F)(F)F)=CN=C1CCNC(=O)C1=CC=CC=C1C(F)(F)F KVDJTXBXMWJJEF-UHFFFAOYSA-N 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 9
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 239000005934 Sulfoxaflor Substances 0.000 description 8
- ZVQOOHYFBIDMTQ-UHFFFAOYSA-N [methyl(oxido){1-[6-(trifluoromethyl)pyridin-3-yl]ethyl}-lambda(6)-sulfanylidene]cyanamide Chemical compound N#CN=S(C)(=O)C(C)C1=CC=C(C(F)(F)F)N=C1 ZVQOOHYFBIDMTQ-UHFFFAOYSA-N 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 5
- 241000482313 Globodera ellingtonae Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 208000031513 cyst Diseases 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 231100000419 toxicity Toxicity 0.000 description 4
- 230000001988 toxicity Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 239000010413 mother solution Substances 0.000 description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
- 229920000053 polysorbate 80 Polymers 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of microbial agents, and particularly relates to an active microbial agent. The active microbial agent is prepared by binary compounding of bacillus cereus and ivermectin according to a certain mass ratio as an active ingredient, wherein the bacillus cereus is 90 hundred million live spores/gram bacillus cereus. When the active ingredients of the active microbial agent are compounded, the active microbial agent has obvious synergistic effect, has higher activity on pests, can improve the control effect on the pests, reduces the pesticide dosage, reduces the pesticide residue of pesticides on crops, simultaneously reduces the environmental pollution, and achieves the control effect of high efficiency, low cost and environmental protection. In addition, the active microbial agent can delay the generation and development of drug resistance of pests and prolong the service life of the agent.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to an active microbial agent.
Background
The soybean cyst nematode is parasitic nematode disease in plants caused by soybean cyst nematode (Heterodera glycines Ichinohe), and has the characteristics of wide distribution, wide host range, long cyst survival time, serious harm and the like. The chemical agent is still the main means for preventing and controlling soybean cyst nematode at present, but in the agricultural production process, the single effective component is used to easily cause the drug resistance of plant diseases and insect pests, which is also the most easily occurring problem in the agricultural plant diseases and insect pests prevention process. When the plant diseases and insect pests generate drug resistance, the control effect of the control agent can be reduced, and the control effect of the agent can be reduced along with the continuous accumulation of drug resistance. By increasing the application dosage of the control agent, the control effect can be improved, but the effect is only temporary, and the development of the drug resistance of the plant diseases and insect pests can be aggravated, so that a vicious circle is formed, and the problems of rampant plant diseases and insect pests, aggravation of environmental pollution, increase of pesticide residue and the like are caused.
The composition of different varieties is compounded, which is a common method for preventing and treating disease and insect damage, but in most cases, the pesticide compounding effect is additive, and the compounding formula truly has a synergistic effect is very few. The compound screening of the existing medicament shows that when bacillus cereus is compounded with fluopyram, paecilomyces lilacinus, sulfoxaflor or ivermectin according to a certain mass ratio, the bacillus cereus and the fluopyram have a synergistic effect on killing soybean cyst nematodes, and the bacillus cereus has important significance on developing medicaments for preventing and treating soybean cyst nematode diseases. At present, no relevant report of the compounding is seen.
Disclosure of Invention
The invention aims to provide an active microbial agent for solving the problem existing in pest control by using a single component.
In order to achieve the above purpose, the present invention provides the following technical solutions:
an active microbial agent, which is prepared by binary combination of bacillus cereus, fluopyram, paecilomyces lilacinus, sulfoxaflor or ivermectin according to a certain mass ratio, wherein the bacillus cereus is 90 hundred million live spores/gram bacillus cereus; the paecilomyces lilacinus is 100 hundred million spores/gram of paecilomyces lilacinus.
Preferably, the mass ratio of 90 hundred million live spores/gram bacillus cereus to fluopyram is 15-20:1.
preferably, the mass ratio of 90 hundred million live spores/gram bacillus cereus to 100 hundred million spores/gram paecilomyces lilacinus is 1-8:16-1.
Preferably, the mass ratio of 90 hundred million live spores/gram bacillus cereus to sulfoxaflor is 1-10:25-1.
Preferably, the mass ratio of 90 hundred million live spores/gram bacillus cereus to ivermectin is 1-14:6-1.
The invention also provides application of the active microbial agent in preventing and controlling nematodes.
Compared with the prior art, the invention has the following beneficial effects:
when the active ingredients of the active microbial agent are compounded, the active microbial agent has obvious synergistic effect, has higher activity on pests, can improve the control effect on the pests, reduces the pesticide dosage, reduces the pesticide residue of pesticides on crops, simultaneously reduces the environmental pollution, and achieves the control effect of high efficiency, low cost and environmental protection. In addition, the active microbial agent can delay the generation and development of drug resistance of pests and prolong the service life of the agent.
Detailed Description
The following is a clear and complete description of the technical solutions of the present patent.
Examples
1. Insect source for test
Collecting disease soil in severe plots of soybean cyst nematodes, separating cysts in a laboratory by adopting a suspension method, picking full fresh cysts under a stereoscopic microscope, cleaning, placing the fresh cysts on wet filter paper for hatching, collecting 2-year-old larvae with consistent ages, and re-suspending the larvae to 150 heads/mL with sterile water for later use.
2. Test agent
90 hundred million live spores/gram bacillus cereus parent medicine (Jiangxi Tian You Biochemical Co., ltd.), 96% fluopyram original medicine (Bayer Co., ltd.), 100 hundred million spores/gram paecilomyces lilacinus parent medicine (De Qiang biological Co., ltd.), 95.9% sulfoxaflor original medicine (Kedi Hua agricultural technology Co., ltd.), 94% ivermectin original medicine (Shanghai Yuan Ye biological technology Co., ltd.)
Preparing a single-dose mother solution from a reagent to be tested, then setting a plurality of groups of proportions, and setting 5 mass concentration gradients for each single dose and each group of proportion mixture for standby. Wherein, when preparing a single-dose mother solution, fluopyram, sulfoxaflor and ivermectin are firstly dissolved by dimethyl sulfoxide and then diluted by tween-80 aqueous solution with the mass fraction of 0.1 percent; 90 hundred million live spores/gram of bacillus cereus and 200 hundred million spores/gram of paecilomyces lilacinus are directly prepared into spore suspension by 0.1 percent of tween-80 aqueous solution, namely mother solution. The medicines are prepared at present.
3. The test method comprises the following steps: (reference to NT/Y1833.1-2009 methods for testing nematode dipping in the pesticide section 1 method for inhibiting plant pathogenic nematodes by means of biological assay in pesticide chambers)
And (3) sucking 4mL of the liquid medicine into a test tube, sucking 4mL of the soybean cyst nematode suspension into the test tube, uniformly mixing the liquid medicine with the nematode suspension, sucking the mixed liquid into the small holes of a 24-hole biochemical test plate by using a liquid transfer pipette, capping, and then culturing at a constant temperature of 25 ℃. Treatment with 0.1% tween-80 in water was used as a blank, and each mass concentration was repeated 2 times. Culturing for 24h, taking 1mL of mixed solution, observing the death condition of nematodes under a dissecting lens, and recording the number of the bus worms and the number of the dead nematodes which are investigated when the number of the nematodes is not less than 100 per repeated observation. Wherein the criterion for nematode death is: the nematodes are stiff and cannot bend when touched by a hairline needle or a bamboo filament needle; from the survey data, mortality and corrected mortality for each treatment were calculated.
In the above formula: p- -mortality in units of; k- -number of dead insects; n- -total number of insects treated.
In the above formula: p (P) 1 -correct mortality in units of; p (P) t -mortality rate in units of treatment; p (P) 0 Blank mortality in%.
4. Data analysis: regression analysis of the log concentration values of each treatment agent and the corrected mortality probability values of each treatment was performed using DPS software to calculate LC of each treatment agent 50 And the co-toxicity coefficient (CTC value) of the mixture was calculated according to the grand cloud Pei method.
5. Evaluation of drug efficacy
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-4.
Table 1 toxicity of Bacillus cereus and Fluopyram to Soy cyst nematode
As can be seen from table 1, at 15-20: in the mass ratio range of 1, the co-toxicity coefficient of bacillus cereus and fluopyram after being compounded is greater than 120, and the bacillus cereus and fluopyram show synergistic effect.
TABLE 2 toxicity of Bacillus cereus and Paecilomyces lilacinus to Soy cyst nematode
As can be seen from table 2, at 1-8:16-1, the co-toxicity coefficient of bacillus cereus and paecilomyces lilacinus after being compounded is greater than 120, and the synergistic effect is shown.
TABLE 3 toxicity of Bacillus cereus and sulfoxaflor to Soy cyst nematode
As can be seen from Table 3, at 1-10: in the mass ratio range of 25-1, the co-toxicity coefficient of bacillus cereus and sulfoxaflor to soybean cyst nematode after being compounded is greater than 120, and the synergistic effect is shown.
Table 4 toxicity of Bacillus cereus and ivermectin against Soy cyst nematode
As can be seen from Table 4, at 1-14: in the mass ratio range of 6-1, the co-toxicity coefficient of bacillus cereus and ivermectin after being compounded on soybean cyst nematode is greater than 120, and the bacillus cereus and ivermectin show synergistic effect.
In conclusion, when the bacillus cereus is compounded with fluopyram, paecilomyces lilacinus, sulfoxaflor or ivermectin according to a certain mass ratio, the bacillus cereus has a synergistic effect on soybean cyst nematodes, and compared with single effective components, the bacillus cereus can improve the control effect and alleviate the problems existing in the use of the single effective components to a certain extent.
Claims (1)
1. The active microbial agent is characterized in that the active microbial agent takes binary compound of bacillus cereus and ivermectin according to a certain mass ratio as an active ingredient, wherein the bacillus cereus is 90 hundred million live spores/gram bacillus cereus, and the mass ratio of the 90 hundred million live spores/gram bacillus cereus to ivermectin is 1-14:6-1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310785034.5A CN116806847B (en) | 2023-06-29 | 2023-06-29 | Active microbial agent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310785034.5A CN116806847B (en) | 2023-06-29 | 2023-06-29 | Active microbial agent |
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| Publication Number | Publication Date |
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| CN116806847A CN116806847A (en) | 2023-09-29 |
| CN116806847B true CN116806847B (en) | 2024-04-02 |
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| CN202310785034.5A Active CN116806847B (en) | 2023-06-29 | 2023-06-29 | Active microbial agent |
Country Status (1)
| Country | Link |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107668054A (en) * | 2016-08-01 | 2018-02-09 | 江苏龙灯化学有限公司 | A kind of nematicidal agent composition and its application method |
| CN115572688A (en) * | 2022-07-18 | 2023-01-06 | 华中农业大学 | Bacillus cereus with high poisoning activity on plant parasitic nematodes and application thereof |
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- 2023-06-29 CN CN202310785034.5A patent/CN116806847B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107668054A (en) * | 2016-08-01 | 2018-02-09 | 江苏龙灯化学有限公司 | A kind of nematicidal agent composition and its application method |
| CN115572688A (en) * | 2022-07-18 | 2023-01-06 | 华中农业大学 | Bacillus cereus with high poisoning activity on plant parasitic nematodes and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 尼秀媚等.四种根结线虫生物防治药剂的田间药效试验.《北方园艺》.2018,90. * |
| 袁红莉，王贺祥.《农业微生物学及实验指导》.中国农业大学出版社,2009,353. * |
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Effective date of registration: 20240506 Address after: No. 1009, Nuanquan Road, Chaoyang Village, Shigu Town, Changge City, Xuchang City, Henan Province, 461000 Patentee after: Henan Aote Agricultural Technology Co.,Ltd. Country or region after: China Address before: No. 10-1, Wenqian Sanxiang, Xiyang District, Huahu Town, Huilai County, Jieyang City, Guangdong Province, China, 515226 Patentee before: Yang Xia Country or region before: China |