CN115956418A - Scopolia anisodus seed germination and seedling raising method - Google Patents
Scopolia anisodus seed germination and seedling raising method Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000007226 seed germination Effects 0.000 title claims abstract description 23
- 241000242873 Scopolia Species 0.000 title description 13
- 241000999530 Anisodus Species 0.000 title description 11
- WTQYWNWRJNXDEG-UHFFFAOYSA-N 6-Hydroxy-hyoscyamin Natural products CN1C(C2)CC(O)C1CC2OC(=O)C(CO)C1=CC=CC=C1 WTQYWNWRJNXDEG-UHFFFAOYSA-N 0.000 claims abstract description 54
- WTQYWNWRJNXDEG-LEOABGAYSA-N anisodamine Chemical compound C1([C@@H](CO)C(=O)O[C@@H]2C[C@H]3[C@@H](O)C[C@@H](C2)N3C)=CC=CC=C1 WTQYWNWRJNXDEG-LEOABGAYSA-N 0.000 claims abstract description 54
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- XEHFSYYAGCUKEN-UHFFFAOYSA-N Dihydroscopoletin Natural products C1CC(=O)OC2=C1C=C(OC)C(O)=C2 XEHFSYYAGCUKEN-UHFFFAOYSA-N 0.000 claims description 23
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention provides a method for seed germination and seedling raising of anisodamine, which comprises the steps of land preparation, seed treatment, sowing and field management. The method can improve the germination rate of the anisodamine seeds and the emergence rate of seedlings, improves the quality of the anisodamine seedlings, and has excellent popularization and application values.
Description
Technical Field
The invention belongs to the field of plant cultivation, and particularly relates to a method for seed germination and seedling raising of anisodamine.
Background
The Tibetan anisodamine is called "Tangyanabao" and is the dried root of Scopolia tangutica (Maxim.) Schner of the genus anisodamine (Anisodus Link et Otto) of the family Solanaceae. Anisodamine is mainly distributed in the plateau areas of Qinghai, tibet and the like, is a modern newly-discovered medicinal resource, and is recorded in the four medical classics, the Jingzhu Bencao, the Tibetan medicine Jingzhu Bencao, the first volume of Tibetan medicine and the Zhonghua Tibetan Bencao, and the Jingzhu Bencao: "atlas" (this book): the black scopolia root is very large; the stem is divided into nine branches, the leaves are black and thick, and the shape of the stem is like a goose wing; purple black flowers, toxic odor, leaf flowers are produced in overground buds, thus becoming 'Tangzai Hei'; thick, hard and bag-shaped fruit pods; small, kidney-shaped, black seeds. Has the effects of killing parasites and treating sores and cutaneous anthrax'. Modern pharmacological research also proves that the main components of anisodamine, anisodamine and the like have the functions of easing pain, relieving spasm, reducing swelling and the like, are mainly used for treating pains, toothache and the like caused by ulcer diseases, acute and chronic gastroenteritis, gastrointestinal neurosis and the like, and are also clinically used for treating chronic tracheitis, so that anisodamine is a Tibetan medicine with high medicinal value.
The market of anisodamine is continuously expanded, the demand is continuously increased, the wild anisodamine has small yield and is not easy to pick, the requirements of people on the anisodamine cannot be met, and the excessive harvesting of the wild anisodamine plants can cause the damage to biological resources and ecological environment. In order to meet the market demand, protect the plateau ecological environment and realize the sustainable utilization of biological resources and the sustainable development of Tibetan medicines, the method is based on the unique biological ecological characteristics of the plateau plant anisodamine, and has very important significance in exploring and developing the introduction and cultivation technology of the anisodamine.
However, anisodamine as a plateau plant adapts to special environmental conditions in plateau areas for a long time, has unique physiological-ecological characteristics, and has a plurality of difficulties in researching the unique plant introduction, seedling raising and cultivation methods, so that the current techniques of anisodamine seedling breeding, introduction, seedling raising and cultivation and the like are still not mature enough and are not systematically and rarely reported.
Disclosure of Invention
The invention aims to provide a scopoletin seed germination and seedling raising method capable of effectively improving the seed germination rate, the emergence rate and the seedling quality of scopoletin.
The invention provides a method for seed germination and seedling raising of anisodamine, which is characterized by comprising the steps of land preparation, seed treatment, sowing and field management, wherein the steps of:
the seed treatment is as follows: soaking anisodamine seeds in a mixed aqueous solution of gibberellin aqueous solution, activated carbon and wood shavings for 15 to 40 hours; the mass ratio of the gibberellin aqueous solution to the activated carbon to the wood shavings is 100: 20-30, wherein the concentration of the gibberellin in the gibberellin aqueous solution is 520-600 mg/L;
the sowing is as follows: in a sunlight greenhouse, carrying out film covering and dibbling on the seeds treated by the seeds at the density of 3-7 kg/mu;
the field management comprises the following steps: covering the dibbled seeds with fine sand, and treating for 12-24 days under the conditions of total darkness and variable temperature, wherein the variable temperature is as follows: maintaining the temperature of 5-10 ℃ for 10-14 h, and then maintaining the temperature of 18-22 ℃ for 10-14 h; then removing the full darkness and the temperature changing condition, then culturing in a sunlight greenhouse for shading by 50-70 percent, and obtaining the anisodamine seedlings after seedling emergence.
Further, the soil preparation is performed so that the seeding depth is 2 to 4cm, preferably 2cm.
Furthermore, in the seed treatment, the mass ratio of the gibberellin aqueous solution to the activated carbon to the wood shavings is 100: 28: 22, wherein the gibberellin concentration in the gibberellin aqueous solution is 550mg/L.
Further, the soaking time for the seed treatment is 36h.
Further, the density of the seeding is as follows: 6 kg/mu.
Further, the film-coated dibble seeding is as follows: covering the whole seedbed with black film, perforating on the film, the hole distance is 4-6 cm, preferably 5cm, and sowing seeds in the film holes.
Further, the temperature-changing conditions of the field management are as follows: maintaining at 10 deg.C for 12 hr, and maintaining at 20 deg.C for 12 hr; the treatment time was 24 days.
Further, the shading for the field management is 50% shading.
Further, the field management also comprises a step of removing the shading condition to strengthen the seedlings after the seedlings are cultivated.
The germination rate and the germination rate of seeds are similar concepts, the germination rate is larger than the germination rate, the germination of the seeds is the basis of germination, but the viability of some seeds is weak, and the seeds may not bud even though the seeds germinate. In the prior art, although a method for improving the germination rate of scopoletin seeds through seed soaking treatment is reported, even if the germination rate is improved, the germination rate, the actual emergence rate, the growth condition and the quality of seedlings are difficult to ensure. The method can improve the germination rate of the anisodamine seeds, simultaneously improve the germination rate, improve the emergence rate of seedlings, improve the quality of the anisodamine seedlings, and has excellent popularization and application values.
The terms of the present invention are explained: "shading" means that the sun shade is used to make the area of the sun light to be exposed to only 50%.
The wood shaving is a product which is formed by pressing agricultural and forestry wastes such as wood chips, bamboo powder, straws and the like serving as raw materials through a high-temperature solid forming of a biomass granulator.
It will be apparent that various other modifications, substitutions and alterations can be made in the present invention without departing from the basic technical concept of the invention as described above, according to the common technical knowledge and common practice in the field.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Drawings
FIG. 1 shows the effect of temperature on the germination of scopolia seeds.
FIG. 2 is a graph showing the effect of illumination on the germination of scopolia seeds.
FIG. 3 shows the germination process of scopoletin seeds under different temperature and illumination conditions.
FIG. 4 is the effect of seeding depth on seedlings of scopoletin.
FIG. 5 is a photograph showing a test of growing seedlings at different densities.
FIG. 6 is a photograph of the seedling emergence of Hyoscyamus anisodus under different covering materials.
FIG. 7 is a photograph of the emergence of seedlings of scopoletin under different sowing modes.
Detailed Description
The anisodamine seeds used in the embodiment of the invention are anisodamine seeds of autonomous state of the carpo Tibetan. The places for seed germination and seedling raising of the embodiment of the invention are as follows: le du county, qinghai province (altitude 1850 m to 4480).
Example 1 cultivation of Anisodus tanguticus by the method of the invention
Land preparation: soil preparation is carried out to ensure that the sowing depth of a field to be sowed is 2cm;
seed treatment: soaking 100kg of scopolia seed in 100kg of gibberellin aqueous solution with the concentration of 550mg/L, 28kg of activated carbon and 22kg of wood chippings for 36 hours;
sowing: covering the treated seeds with a film at the density of 6 kg/mu, dibbling the seeds to a sunlight greenhouse, and then covering the seeds with fine sand; the film covering and dibbling method comprises the following steps: covering all seedbeds with black film, perforating on the film with hole distance of 5cm, and sowing seeds in the film holes.
Field management: maintaining at 10 ℃ for 12h and at 20 ℃ for 12h under the full dark condition; after 24 days of treatment, removing the conditions of complete darkness and variable temperature, culturing in a normal sunlight greenhouse environment in a shady mode for 50 percent, obtaining scopoletin seedlings after seedling emergence, and removing the shady condition to strengthen seedlings.
Experimental example 1 seed Germination experiment
Taking 1kg of anisodamine seeds, dividing into 4 groups, soaking in gibberellin aqueous solution with gibberellin concentration of 550mg/L, and respectively soaking in mixed aqueous solution of activated carbon and wood shavings at different proportions. Specific mass ratios are shown in the following table, for example, then the seeds after 4 groups of treatments are respectively coated and dibbled on the same land, and the coating dibbling is that: covering all seedbeds with black film, perforating on the film with hole distance of 5cm, and sowing seeds in the film holes. Seed germination rates were calculated as follows in table 1:
TABLE 1
As can be seen from the above table, in the seed treatment, the optimum mass ratio of the gibberellin aqueous solution, the activated carbon and the wood shavings was 100: 28: 22, and the gibberellin concentration in the gibberellin aqueous solution was 550mg/L. Can obtain better seed germination rate.
Experimental example 2 screening of seed Germination conditions
1. Influence of different temperature treatments on the seed germination of scopolia acutangula
The anisodamine seeds were treated at constant temperature (20 deg.C, 25 deg.C), variable temperature (5 deg.C (12 h)/20 deg.C (12 h), 10 deg.C (12 h)/20 deg.C (12 h)), and the rest of the treatment was the same as in example 1. And recording the seed germination process, and calculating the germination vigor, the germination rate and the germination index.
At germination day 5, the results obtained are shown in fig. 1, table 2:
TABLE 2 Effect of temperature on Scopolia tangutica seed germination
As can be seen, the temperature-variable treatment of the scopoletin seed germination rate is higher than the temperature-variable treatment, wherein the germination vigor (day 5), the germination rate and the germination index are respectively 36.88 ± 3.55%, 65.00 ± 0.51% and 3.53 ± 0.72% which are the highest in the temperature-variable treatment of 10 ℃ (12 h)/20 ℃ (12 h), so the temperature-variable treatment method of promoting the scopoletin seed germination by the temperature-variable treatment is preferred, and the temperature-variable treatment method of 10 ℃ (12 h)/20 ℃ (12 h) is more preferred.
2. Influence of different light-dark ratio treatment on anisodamine seed germination
Under the condition of temperature of 10 ℃ (12 h)/20 ℃ (12 h), respectively adopting the following steps within 24 h: the anisodamine seeds are treated in the full darkness, 16h plus or minus light for 8h, 12h in darkness plus light for 12h, 8h in darkness plus light for 16h, and the full light condition, and the other treatment modes are the same as the embodiment 1. And (4) recording the seed germination process, and calculating the germination vigor and the germination rate.
At germination day 5, the results are shown in fig. 2, table 3:
TABLE 3 Effect of illumination time on Scopolia anisodus seed Germination
Therefore, under the condition that the temperature is 10 ℃ (12 h)/20 ℃ (12 h), different illumination time has significant influence on the germination vigor and the germination rate of anisodamine seeds. The germination vigor and germination rate of the dark treatment group are significantly higher than those of other light treatments.
3. Scopolia tangutica seed germination process
As can be seen from FIG. 3, under the 5 treatment conditions of the experiment, the scopoletin anisodus seeds all start to germinate on the 9 th day, reach the germination peak period on the 12 th to 16 th days, and finish germination after 23 days. Therefore, the actual germination treatment (total darkness + variable temperature treatment) time is preferably 24 days.
Experimental example 3 Effect of sowing on the raising of Hyoscyamus anisodus seedlings
1. Influence of sowing depth on anisodamine seedlings
The seeds were cleaned and sown in different covering depths (0 cm, 0.5cm, 1cm, 2cm, 4cm, 5cm, 6cm, 8 cm) with large seeds sieved with 0.2cm sieve, and the rest of the treatment was the same as in example 1. Statistics are carried out on the growth condition of seedlings, the emergence rate and the seedling quality, and the results are shown in figure 4, tables 4-5 and figure 1:
TABLE 4 influence of sowing depth on the growth and emergence of seedlings of scopoletin seeds
| Depth of soil covering | Plant height/cm | Root diameter/mm | Root length/cm | Rate of emergence/% |
| 0cm | 3.60±0.21 | 2.38±0.14 | 9.26±1.12 | 10.50±1.89 |
| 0.5cm | 4.46±0.21 | 2.65±0.10 | 10.23±0.60 | 80.45±2.90 |
| 1em | 5.17±0.18 | 2.94±0.10 | 12.06±0.68 | 93.89±2.00 |
| 2cm | 5.24±0.30 | 3.07±0.12 | 13.28±0.42 | 87.50±2.79 |
| 4cm | 7.35±0.38 | 3.76±0.14 | 12.24±0.43 | 72.00±4.36 |
| 5cm | 5.65±0.14 | 3.51±0.15 | 12.14±0.66 | 57.50±3.44 |
| 6cm | 5.53±0.32 | 2.74±0.15 | 12.44±0.76 | 50.63±3.33 |
| 8cm | 3.61±0.60 | 2.47±0.17 | - | 10.56±4.03 |
| F (7，72) | 16.471 | 12.816 | 4.343 | 103.452 |
| P | 0.00 | 0.00 | 0.00 | 0.00 |
TABLE 5 Effect of sowing depth on Scopolia tangutica seedling Biomass
As can be seen, the sowing depth has significant influence on the emergence and growth of anisodamine (P is less than 0.05). The highest emergence rate is achieved by treating the seeds with the sowing depth of 1cm, the average emergence rate is 93.89% (the seeds in the test are large-grained seeds which are cleaned and sieved by 0.2 cm), the root biomass (0.0717 g/plant) accumulation and the root-crown ratio are highest by treating the seeds with the sowing depth of 2cm, and the highest average total biomass is 0.2408 g/plant by treating the seeds with the sowing depth of 4cm.
And (3) carrying out comprehensive weighted scoring on the emergence and growth influence of anisodamine by different sowing depths: selecting 3 indexes of aboveground part, root biomass and emergence rate, carrying out comprehensive weighted scoring on the emergence and growth conditions of the scopolia with different sowing depths, wherein tables 6 and 7 show that the weighted scoring is highest when the sowing depth is 2cm, and then the sowing depth is 4cm, so that the production reality is promoted to be combined, the earthing depth of the scopolia seeds is preferably 2-4 cm during sowing, the deepest cannot exceed 5cm, and the seeds must be covered with soil after sowing.
TABLE 6 weighted Scoring criteria
TABLE 7 depth of seeding scoring for emergence effect
2. Influence of seeding density on emergence rate of anisodamine seedlings
After the seed soaking treatment for 36 hours, the seeds were sown in the following table, and the rest of the treatment was the same as in example 1. The seeding experiment process is shown in fig. 5:
M1-M5 represent cultivation densities:
m1:3 kg/mu; and M2:4 kg/mu; and M3:5 kg/mu; m4:6 kg/mu; m5: and 7 kg/mu, performing seedling culture, and calculating the seedling yield of about 10 ten thousand plants/mu according to the weight of each thousand seeds of 6.70g and the seed germination rate of 85 percent.
And counting the number of seedlings per square meter after seedling growing to seedling emergence.
The results are shown in Table 8:
table 8 influence of seeding density on the emergence rate of scopolia seedlings (m) 2 )
It can be seen that the number of seedlings per square meter increases as the seeding density increases, but the number of seedlings is decreased when the density is too high, and the number of seedlings is the highest when the density is 6 kg/mu, and thus the amount of seeding density of 6 kg/mu is preferable.
3. Influence of covering material on the rate of emergence of anisodamine seedlings
3.1 Experimental methods
The seeds are sowed after being soaked for 36 hours, the sowing amount is 6 kg/mu, and different covering materials are used:
f1: wheat straw; f2: sawdust; f3: fine sand, seedling raising, and counting the number of seedlings per square meter after seedling raising and seedling emergence.
3.2 results of the experiment
The effect of the covering material on the rate of emergence of the scopoletin seedlings is shown in fig. 6 and table 9:
TABLE 9 influence of the covering materials on the emergence rate of seedlings of scopoletin (m) 2 )
| F1 | F2 | F3 | |
| Number of seedlings/m 2 | 358.67±16.65 | 508±86.53 | 654.67±224.58 |
Therefore, fine sand coverage is favorable for the seedling emergence of scopoletin seeds, sawdust coverage is inferior, and wheat straw coverage is poor, so fine sand coverage is preferred.
4. Influence of sowing mode on emergence rate of anisodamine seedlings
4.1 Experimental methods
After the seeds are soaked for 36 hours, the seeds are sown according to the following sowing mode, the sowing quantity is 6 kg/mu, and fine sand is adopted for covering:
b1: and (3) black film mulching and dibbling: covering with a whole film, and punching holes on the film at a pitch of 5cm; b2: black film covering and drilling: the width of the film is 20cm, and the space between films is 10cm; b3: broadcasting: and (3) growing seedlings in the furrow with the width of 60cm and the length of 20cm between films and the height of about 20cm, and counting the number of seedlings grown per square meter after seedling growing.
4.2 results of the experiment
The effect of the sowing pattern on the rate of emergence of scopoletin seedlings is shown in fig. 7 and table 10:
TABLE 10 influence of the sowing pattern on the emergence rate of Anisodus tanguticus seedlings (m) 2 )
| B1 | B2 | B3 | |
| Number of seedlings/m 2 | 652.0±214.69 | 413.33±99.94 | 482.67±61.10 |
Therefore, the film-coated dibbling is favorable for the emergence of seedlings of the scopolia seeds, the film-coated drill sowing is repeated, and the sowing effect is poor, so that the cultivation in the film-coated dibbling mode is preferably adopted.
Experimental example 4 Effect of opacity and seed size on the growth of seedlings of Anisodus tanguticus
The anisodamine seeds are classified according to a sieve with 8 meshes, the weight of the large seeds in the sieve is 0.8388g, the average weight of the small seeds in the sieve is 0.6496g, the anisodamine seeds are treated and sowed according to the conditions determined in the experimental examples 1 and 2, then the seedlings are grown according to different shading conditions, and the other treatment modes are the same as the example 1.
The influence of different shade degrees and seed sizes on the growth of the seedlings of anisodamine is shown in tables 11-14.
TABLE 11 influence of opacity and seed size on the growth of seedlings of anisodamine
TABLE 12 Effect of opacity and seed size on the growth of seedlings of Anisodus tanguticus
Experiments research that the germination rate and the seedling rate of the scopoletin anisodus have no correlation with the size of the seeds, but are related to the environment, and 50 percent of shading is favorable for the seedling formation of the scopoletin anisodus seeds.
Different light tests prove that the germination rate and the seedling rate of the scopoletin anisodus are not related to the size of the seeds, but are related to the environment, and 50 percent of shading is beneficial to the seedling formation of the scopoletin anisodus seeds.
TABLE 13 influence of different illumination and seed size on the biomass of scopolia seedlings
TABLE 14 Effect of different illumination and seed size on the biomass of seedlings of anisodamine
The total biomass accumulation of the anisodamine seedlings has no correlation with the shading degree and the sizes of the seeds, different illumination influences the biomass distribution of the anisodamine seedlings, the biomass accumulation is influenced by the combined action of illumination conditions and the sizes of the seeds, proper shading needs to be considered in the anisodamine seedling culture process, the seedling culture rate is favorably improved, and after the seedlings emerge and grow, the shading net needs to be unfastened, so that the strong seedlings are favorably realized.
Experimental example 5 influence of different seedling growing environments on the seedling and quality of anisodamine
As can be seen from Table 15, the seedlings were grown in different seedling growing environments, and the other treatments were the same as in example 1. Different seedling culture environments have obvious influence on the seedling rate and morphological characteristics of anisodamine. The plateau sunlight greenhouse obviously improves the seedling rate, the root diameter, the plant height and the root length (P is less than 0.01) of the scopoletin seedlings, the seedling rate is 42.5 percent and is more than 2 times of the seedling rate of 20.21 percent of the field seedling, and the greenhouse condition is more favorable for the seedling rate and the growth of the scopoletin seedlings.
TABLE 15 seedling rate and growth of anisodamine greenhouse seedlings
As can be seen from Table 15, different seedling growing environments have significant effects on the accumulation of anisodamine biomass. The total biomass of anisodamine greenhouse seedlings (0.4788 g/plant) was 4 times that of the field seedlings (0.1188 g/plant). The biological components of stems, leaves and roots have significant difference (P is less than 0.01), especially the biomass of greenhouse seedling leaves (0.6700 g/plant) is more than 10 times of the biomass of field seedling (0.069 g/plant), and the biomass of underground roots (0.1679 +/-0.02 g/plant) is more than 2 times of the biomass of field seedling (0.0745 +/-0.01 g/plant). Research shows that the seedling rate of the scopoletin seeds for direct seeding in the field is lower, the seedling rate and biomass of seedling culture are obviously improved in a greenhouse, and the seedling rate and biomass are respectively improved by more than 1 time and more than 3 times. The greenhouse seedling culture accelerates the biomass accumulation, is favorable for further field transplanting of the seedlings and shortens the seedling culture time.
In conclusion, the method can improve the germination rate of the anisodamine seeds and the emergence rate of seedlings, is beneficial to biomass accumulation of the anisodamine, improves the quality of the anisodamine seedlings, and has excellent popularization and application values.
Claims (10)
1. A method for seed germination and seedling raising of anisodamine is characterized by comprising the steps of land preparation, seed treatment, sowing and field management, wherein:
the seed treatment comprises the following steps: soaking scopoletin seeds in a mixed aqueous solution of gibberellin aqueous solution, activated carbon and wood shavings for 15-40 hours; the mass ratio of the gibberellin aqueous solution to the activated carbon to the wood shavings is 100: 20-30, wherein the concentration of the gibberellin in the gibberellin aqueous solution is 520-600 mg/L;
the sowing is as follows: in a sunlight greenhouse, carrying out film covering and dibbling on the seeds treated by the seeds at the density of 3-7 kg/mu;
the field management comprises the following steps: covering the dibbled seeds with fine sand, and treating for 12-24 days under the conditions of total darkness and variable temperature, wherein the variable temperature is as follows: maintaining the temperature of 5-10 ℃ for 10-14 h, and then maintaining the temperature of 18-22 ℃ for 10-14 h; then removing the full darkness and the temperature changing condition, then culturing in a sunlight greenhouse for shading by 50-70 percent, and obtaining the anisodamine seedlings after seedling emergence.
2. The method of claim 1, wherein said soil preparation results in a seeding depth of 2 to 4cm.
3. The method of claim 2 wherein said soil preparation results in a seed depth of 2cm.
4. The method of claim 1, wherein the seed treatment is carried out at a ratio of 100: 28: 22 by mass of the aqueous solution of gibberellin to the activated charcoal, wherein the concentration of gibberellin in the aqueous solution of gibberellin is 550mg/L.
5. The method of claim 1, wherein the seed is treated for a soaking time of 36 hours.
6. The method of claim 1, wherein the density of seeding is: 6 kg/mu.
7. The method of claim 1, wherein the film-coated dibble seeding is: covering the whole seedbed with black film, perforating on the film, the hole distance is 4-6 cm, preferably 5cm, and sowing seeds in the film holes.
8. A seedling raising method according to claim 1, characterized in that the temperature-changing conditions for field management are: maintaining at 10 ℃ for 12h, and then maintaining at 20 ℃ for 12h; total darkness and variable temperature treatment time were 24 days.
9. A method of growing seedlings according to claim 1 in which the shade of the field management is 50% shade.
10. A seedling raising method as claimed in any one of claims 1 to 9, characterized in that the field management further comprises a step of removing shading conditions to strengthen the seedlings after the seedlings are raised.
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| CN116724887A (en) * | 2023-08-15 | 2023-09-12 | 成都第一制药有限公司 | Efficient induction method of anisodamine polyploid |
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Cited By (2)
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| CN116724887A (en) * | 2023-08-15 | 2023-09-12 | 成都第一制药有限公司 | Efficient induction method of anisodamine polyploid |
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