CN115316278A - Seed propagation technology of atractylodes lancea of Compositae - Google Patents
Seed propagation technology of atractylodes lancea of Compositae Download PDFInfo
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- CN115316278A CN115316278A CN202211110309.7A CN202211110309A CN115316278A CN 115316278 A CN115316278 A CN 115316278A CN 202211110309 A CN202211110309 A CN 202211110309A CN 115316278 A CN115316278 A CN 115316278A
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- rhizoma atractylodis
- compositae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to the technical field of rhizoma atractylodis propagation, in particular to a propagation technology of seeds of rhizoma atractylodis of Compositae. The method comprises the following steps: preparing seeds; seedling culture; inducing cluster buds; dividing the induced cluster buds into single plants; selecting a planting land; weeding, topdressing and watering; and (6) harvesting. The invention provides a seed propagation technology of a composite plant rhizoma atractylodis, which can be directly used for industrialized large-scale seedling production, has the advantages of less pollution, high propagation speed, low mutation rate, regular and robust seedlings, low production cost, convenient transportation, high transplanting survival rate and the like, and has the advantages of uniform seedlings, robust growth, strong resistance, better growth potential than seedlings cultivated by seeds after being transplanted to a field and capability of keeping the excellent characters of a clone before cultivation.
Description
Technical Field
The invention relates to the technical field of rhizoma atractylodis propagation, in particular to a seed propagation technology of rhizoma atractylodis of Compositae.
Background
Rhizoma Atractylodis (Atractylodes lancea (Thunb.) DC) is a Compositae plant, and is a perennial herb. Rhizoma atractylodis is a genuine Chinese medicinal material, can be used for treating dyspepsia, stomachache, nyctalopia and other symptoms, and continuously enriches the pharmacological understanding of human beings by scientific research. People can also use the medicine to prevent cold and SARS, and treat sinus tachycardia, diabetes, bacillary dysentery, etc.
However, due to the damage of ecological environment and the predatory mining and digging in the past, the resources of wild rhizoma atractylodis are increasingly scarce and are in an endangered state. Rhizoma atractylodis is valuable and unavailable in the medicinal material market.
The existing rhizoma atractylodis is one of the bulk medicinal materials in China, has high medicinal value and great market demand, is cultivated in various parts of China, generally takes seed propagation as the main part during planting, is extremely key in seedling cultivation during planting and is related to yield and quality, and the existing rhizoma atractylodis is not provided with a systematic cultivation and planting system in a self-seedling stage in most planting processes, so that the planting is easy to suffer certain loss.
Disclosure of Invention
The invention aims to provide a propagation technology of seeds of Atractylodes lancea of Compositae, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a seed propagation technology of Atractylodes lancea of Compositae comprises the following steps:
1) Preparing seeds, namely selecting large and plump rhizoma atractylodis seeds, washing the large and plump rhizoma atractylodis seeds with a detergent and water for 20min, soaking the large and plump rhizoma atractylodis seeds in 75% alcohol for 20 to 30 min, taking the rhizoma atractylodis seeds out, soaking the rhizoma atractylodis seeds in potassium permanganate for disinfection, and washing the rhizoma atractylodis seeds with sterile water for 3 to 5 times;
2) Seedling culture, namely inoculating the treated atractylis lancea seeds on a germination culture medium to culture for 1-2 weeks to grow seedlings;
3) Inducing cluster buds, after normal culture, germinating rhizoma Atractylodis seeds and inducing into rhizoma Atractylodis callus with diameter of 0.2-0.4 cm;
4) Dividing the induced cluster buds into individual plants on a super clean bench, cutting the individual plants with high branches into stem sections with buds by using sterilized scissors, transferring the stem sections with the rest cluster buds into a 1/2MS culture medium and the culture medium, wherein the culture temperature is 28-30 ℃, the illumination intensity is 1600Lx, the illumination time is 6 hours/day, and the stem sections naturally root and grow into complete plants;
5) Selecting a planting land, selecting a land block which is fertile, loose, sufficient in illumination and good in drainage as a seedling raising land and is suitable for humus soil or sandy loam, and the exposed barren mountain or barren slope land;
6) Weeding in time after transplanting, increasing watering times after topdressing, cleaning drainage ditches in rainy seasons, draining accumulated water and avoiding root rot;
7) Harvesting, selecting seeds of healthy high-quality plants 2-3 years after autumn, winnowing, screening and removing the seeds with shrivelled, small and low germination rate, and cultivating the obtained high-quality seeds.
Further, the rhizoma atractylodis seeds are soaked in 3% ammonia water for 5-10 minutes after being soaked and disinfected in potassium permanganate, and then are washed by sterile water.
Further, the atractylodes rhizome seeds are directly induced to bud on MS culture medium added with hormone, wherein MS is added with 3 percent of sucrose and 0.8 percent of agar in the minimal medium.
Further, moving in late 4 months, before planting and sowing, applying base fertilizer and ploughing again, finely harrowing and leveling to form a furrow with the width of 1 meter, carrying out drill sowing or broadcast sowing, and transversely ditching on the furrow surface by drill sowing with the furrow distance of 20-25 cm and the furrow depth of 3 cm.
Further, the base fertilizer comprises rapeseed cakes, cow dung, calcium phosphate and rice hulls.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a seed propagation technology of a composite plant rhizoma atractylodis, which can be directly used for industrialized large-scale seedling production, has the advantages of less pollution, high propagation speed, low mutation rate, regular and robust seedlings, low production cost, convenient transportation, high transplanting survival rate and the like, and has the advantages of uniform seedlings, robust growth, strong resistance, better growth potential than seedlings cultivated by seeds after being transplanted to a field and capability of keeping the excellent characters of a clone before cultivation.
Detailed Description
The technical solution of the present invention is further illustrated by the following specific examples.
Example one
The invention provides a seed propagation technology of a composite plant rhizoma atractylodis, which comprises the following steps:
1) Preparing seeds, namely selecting large and plump rhizoma atractylodis seeds, washing the large and plump rhizoma atractylodis seeds with a detergent and water for 20min, soaking the large and plump rhizoma atractylodis seeds in 75% alcohol for 20min, taking the rhizoma atractylodis seeds out, soaking the rhizoma atractylodis seeds in potassium permanganate for disinfection, soaking the rhizoma atractylodis seeds in 3% ammonia water for 5 min after soaking the rhizoma atractylodis seeds in potassium permanganate for disinfection, washing the rhizoma atractylodis seeds with sterile water, and washing the rhizoma atractylodis seeds with sterile water for 3 times;
2) Seedling culture, namely inoculating the treated atractylis lancea seeds on a germination culture medium to culture for 1 week to obtain seedlings;
3) Inducing cluster buds, after normal culture is finished, germinating rhizoma atractylodis seeds and directly inducing buds on an MS culture medium added with hormone, wherein the basic culture medium selects MS added with 3 percent of sucrose and 0.8 percent of agar to be induced into rhizoma atractylodis callus with the diameter of 0.2 cm;
4) Dividing the induced cluster buds into individual plants on a super clean bench, cutting the individual plants with high branches into stem sections with buds by using sterilized scissors, transferring the stem sections with the rest of the individual plants of the cluster buds into a 1/2MS culture medium and the culture medium, culturing at 28 ℃, with the illumination intensity of 1600Lx and the illumination time of 6 hours/day, naturally rooting and growing into complete plants;
5) Selecting a planting land, selecting a land block which is fertile, loose, sufficient in illumination and good in drainage in the sunny barren mountain or barren slope land as a seedling raising land, and preferably using humus soil or sandy loam;
6) Moving in the last ten days of 4 months, applying base fertilizer before planting and sowing, ploughing again, finely harrowing and leveling to form a furrow with the width of 1 meter, carrying out drill sowing or broadcast sowing, transversely ditching on the surface of the furrow by drill sowing, wherein the furrow distance is 20 cm, the furrow depth is 3 cm, weeding is carried out in time after transplanting, watering times are increased after topdressing, a drainage ditch needs to be cleaned in rainy seasons, accumulated water is drained, and root rot is avoided, wherein the base fertilizer comprises rape seed cakes, cow dung, calcium phosphate and rice husks;
7) Harvesting, selecting seeds of healthy high-quality plants after 2 years of autumn, winnowing the seeds, screening, removing the seeds with shrivelled, small and low germination rate, and cultivating the obtained high-quality seeds.
Example two:
the invention provides a seed propagation technology of a composite plant rhizoma atractylodis, which comprises the following steps:
1) Preparing seeds, namely selecting large and plump rhizoma atractylodis seeds, washing the large and plump rhizoma atractylodis seeds with a detergent and water for 20min, soaking the large and plump rhizoma atractylodis seeds in 75% alcohol for 20min, taking the rhizoma atractylodis seeds out, soaking the rhizoma atractylodis seeds in potassium permanganate for disinfection, soaking the rhizoma atractylodis seeds in ammonia water with the mass concentration of 3% for 10 min after soaking the rhizoma atractylodis seeds in the potassium permanganate for disinfection, then washing the rhizoma atractylodis seeds with sterile water, and then washing the rhizoma atractylodis seeds with sterile water for 5 times;
2) Seedling culture, namely inoculating the treated atractylis lancea seeds on a germination culture medium to culture for 2 weeks to grow seedlings;
3) Inducing cluster buds, after normal culture is finished, germinating rhizoma atractylodis seeds and directly inducing buds on an MS culture medium added with hormone, wherein the basic culture medium selects MS added with 3 percent of sucrose and 0.8 percent of agar to be induced into rhizoma atractylodis callus with the diameter of 0.4 cm;
4) Dividing the induced multiple shoots into single plants on a super clean bench, cutting the single plants with high branches into stem sections with buds by using sterilized scissors, transferring the stem sections with the rest multiple shoots into a 1/2MS culture medium together with the rest multiple shoots, culturing at the temperature of 30 ℃, with the illumination intensity of 1600Lx and the illumination time of 6 hours/day, naturally rooting, and growing into complete plants;
5) Selecting a planting land, selecting a land block which is fertile, loose, sufficient in illumination and good in drainage as a seedling raising land and is suitable for humus soil or sandy loam, and the exposed barren mountain or barren slope land;
6) Moving in the last ten days of 4 months, applying base fertilizer before planting and sowing, ploughing again, finely harrowing and leveling to form a furrow with the width of 1 meter, carrying out drill sowing or broadcast sowing, transversely ditching on the surface of the furrow by drill sowing, wherein the furrow distance is 25 cm, the furrow depth is 3 cm, weeding is carried out in time after transplanting, watering times are increased after topdressing, a drainage ditch needs to be cleaned in rainy seasons, accumulated water is drained, and root rot is avoided, wherein the base fertilizer comprises rape seed cakes, cow dung, calcium phosphate and rice husks;
7) Harvesting, selecting seeds of healthy high-quality plants after 3 years of autumn, winnowing the seeds, screening, removing the seeds with shrivelled, small and low germination rate, and cultivating the obtained high-quality seeds.
The above-described embodiments are merely some preferred embodiments of the present invention, and those skilled in the art can make various alternative modifications and combinations to the above-described embodiments based on the technical solution of the present invention and the related teachings of the above-described embodiments.
Claims (5)
1. A seed propagation technology of Atractylodes lancea of Compositae is characterized by comprising the following steps:
1) Preparing seeds, namely selecting large and plump rhizoma atractylodis seeds, washing the large and plump rhizoma atractylodis seeds with a detergent and water for 20min, soaking the large and plump rhizoma atractylodis seeds in 75% alcohol for 20 to 30 min, taking the rhizoma atractylodis seeds out, soaking the rhizoma atractylodis seeds in potassium permanganate for disinfection, and washing the rhizoma atractylodis seeds with sterile water for 3 to 5 times;
2) Seedling culture, namely inoculating the treated atractylis lancea seeds on a germination culture medium to culture for 1-2 weeks to grow seedlings;
3) Inducing cluster buds, after normal culture, germinating rhizoma Atractylodis seeds and inducing into rhizoma Atractylodis callus with diameter of 0.2-0.4 cm;
4) Dividing the induced cluster buds into individual plants on a super clean bench, cutting the individual plants with high branches into stem sections with buds by using sterilized scissors, transferring the stem sections with the rest cluster buds into a 1/2MS culture medium and the culture medium, wherein the culture temperature is 28-30 ℃, the illumination intensity is 1600Lx, the illumination time is 6 hours/day, and the stem sections naturally root and grow into complete plants;
5) Selecting a planting land, selecting a land block which is fertile, loose, sufficient in illumination and good in drainage in the sunny barren mountain or barren slope land as a seedling raising land, and preferably using humus soil or sandy loam;
6) Weeding in time after transplanting, increasing watering times after topdressing, cleaning drainage ditches in rainy seasons, draining accumulated water and avoiding root rot;
7) Harvesting, selecting seeds of healthy high-quality plants 2-3 years after autumn, winnowing, screening and removing the seeds with shrivelled, small and low germination rate, and cultivating the obtained high-quality seeds.
2. The technique of claim 1, wherein the seed of Atractylodes lancea of Compositae is selected from the group consisting of: soaking the rhizoma atractylodis seeds in 3% ammonia water for 5-10 minutes after soaking and sterilizing the rhizoma atractylodis seeds in potassium permanganate, and then washing with sterile water.
3. The technique of claim 1, wherein the seed of Atractylodes lancea of Compositae is selected from the group consisting of: the rhizoma atractylodis seed is directly induced to bud on MS culture medium added with hormone, wherein the MS culture medium is added with 3 percent of sucrose and 0.8 percent of agar.
4. The technique of claim 1, wherein the seed of Atractylodes lancea of Compositae is selected from the group consisting of: moving in late 4 months, applying base fertilizer before planting and sowing, finely harrowing, making a furrow with the width of 1 meter, carrying out drill sowing or broadcast sowing, and transversely ditching on the furrow surface by drill sowing with the furrow distance of 20-25 cm and the furrow depth of 3 cm.
5. The technique of claim 4, wherein the seed of Atractylodes lancea of Compositae is selected from the group consisting of: the base fertilizer comprises rapeseed cakes, cow dung, calcium phosphate and rice hulls.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116267620A (en) * | 2023-04-21 | 2023-06-23 | 德兴市中医研究院试验培训基地 | Method for constructing callus induction and adventitious bud regeneration system by utilizing atractylis lancea leaf stalks |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960129A (en) * | 2014-04-24 | 2014-08-06 | 江苏农林职业技术学院 | Atractylis lancea tissue culturing and rapid propagating method |
| CN105104211A (en) * | 2015-10-10 | 2015-12-02 | 山西省农业科学院作物科学研究所 | Tissue culture and rapid propagation method of Atractylodes chinensis |
| CN105284612A (en) * | 2015-09-30 | 2016-02-03 | 江苏农林职业技术学院 | Method for rapidly propagating rhizoma areactylodis lanceae test-tube plantlet |
| CN105993956A (en) * | 2016-06-14 | 2016-10-12 | 江苏茅山地道中药材种植有限公司 | Fast propagating method for atractylis lancea |
| WO2021036472A1 (en) * | 2019-08-26 | 2021-03-04 | 南京农业大学 | Method for improving quality and transplanting survival rate of atractylodes lancea test-tube seedlings |
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2022
- 2022-09-13 CN CN202211110309.7A patent/CN115316278A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103960129A (en) * | 2014-04-24 | 2014-08-06 | 江苏农林职业技术学院 | Atractylis lancea tissue culturing and rapid propagating method |
| CN105284612A (en) * | 2015-09-30 | 2016-02-03 | 江苏农林职业技术学院 | Method for rapidly propagating rhizoma areactylodis lanceae test-tube plantlet |
| CN105104211A (en) * | 2015-10-10 | 2015-12-02 | 山西省农业科学院作物科学研究所 | Tissue culture and rapid propagation method of Atractylodes chinensis |
| CN105993956A (en) * | 2016-06-14 | 2016-10-12 | 江苏茅山地道中药材种植有限公司 | Fast propagating method for atractylis lancea |
| WO2021036472A1 (en) * | 2019-08-26 | 2021-03-04 | 南京农业大学 | Method for improving quality and transplanting survival rate of atractylodes lancea test-tube seedlings |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116267620A (en) * | 2023-04-21 | 2023-06-23 | 德兴市中医研究院试验培训基地 | Method for constructing callus induction and adventitious bud regeneration system by utilizing atractylis lancea leaf stalks |
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