CN115886016A - A composition containing berberine - Google Patents
A composition containing berberine Download PDFInfo
- Publication number
- CN115886016A CN115886016A CN202211578920.2A CN202211578920A CN115886016A CN 115886016 A CN115886016 A CN 115886016A CN 202211578920 A CN202211578920 A CN 202211578920A CN 115886016 A CN115886016 A CN 115886016A
- Authority
- CN
- China
- Prior art keywords
- berberine
- citrus
- sodium
- disease
- sodium benzoate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 229940093265 berberine Drugs 0.000 title claims abstract description 36
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 239000000203 mixture Substances 0.000 title claims abstract description 14
- 241000207199 Citrus Species 0.000 claims abstract description 19
- 235000020971 citrus fruits Nutrition 0.000 claims abstract description 19
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 15
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 15
- 239000011734 sodium Substances 0.000 claims abstract description 15
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 14
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 14
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 13
- 208000019756 lichen disease Diseases 0.000 claims description 12
- 238000013329 compounding Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 16
- 241000195493 Cryptophyta Species 0.000 abstract description 6
- 230000001717 pathogenic effect Effects 0.000 abstract description 6
- 239000000575 pesticide Substances 0.000 abstract description 6
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 230000001934 delay Effects 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 239000003814 drug Substances 0.000 description 14
- 201000010099 disease Diseases 0.000 description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 10
- 229940079593 drug Drugs 0.000 description 10
- 230000002265 prevention Effects 0.000 description 10
- 238000011282 treatment Methods 0.000 description 10
- 238000012360 testing method Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 241000223665 Phaeococcus Species 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000011835 investigation Methods 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 206010039509 Scab Diseases 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241000195628 Chlorophyta Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000002420 orchard Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OICGFSUWXCJLCO-UHFFFAOYSA-N 1-ethylsulfonylsulfanylethane Chemical compound CCSS(=O)(=O)CC OICGFSUWXCJLCO-UHFFFAOYSA-N 0.000 description 1
- 241000832615 Apatococcus lobatus Species 0.000 description 1
- 241000133570 Berberidaceae Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 241001672694 Citrus reticulata Species 0.000 description 1
- 239000005760 Difenoconazole Substances 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- 239000005802 Mancozeb Substances 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 241000694540 Pluvialis Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 210000001700 mitochondrial membrane Anatomy 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- -1 sodium benzoate compound Chemical class 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a berberine-containing composition. A composition containing berberine comprises berberine and sodium benzoate or sodium rosinate as effective components. When the berberine is compounded with the sodium benzoate or the sodium rosinate, the compound preparation shows good synergistic effect on the unreal algae, can improve the control effect on the citrus lichen, can reduce the application dosage of pesticide, and delays the generation of drug resistance of pathogenic algae.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a composition containing berberine.
Background
In citrus orchard, a layer of green clothes is often seen on the trunk, branches, leaves and fruits even on the ground, the green clothes is a lichen disease, and the pathogen is Phaeococcus pseudopluvialis (Apatococcus lobatus) of Phaeococcus of Chlorophyta family. The moss is mainly damaged in the middle and lower parts of the tree crown, and the whole tree is damaged when the moss is serious, so that the photosynthesis of the tree body and the appearance and quality of fruits are seriously influenced. The production usually adopts bactericide such as mancozeb, difenoconazole, iprodione or ethylicin to prevent and treat, and with the long-term use of the bactericide, pathogens generate drug resistance, so that the prevention and treatment effect is not ideal.
Berberine is also called berberine, is a phenylpropanoid quaternary alkaloid with molecular formula C 20 H 18 NO 4 It is a yellow needle crystal, with melting point of 145 deg.C, and is soluble in water and insoluble in benzene, ether and chloroform. It is contained in many plants of berberidaceae and the like. Due to the broad-spectrum antibacterial effect, berberine is found to be effective in preventing and treating the lichen diseases in recent years by destroying the integrity of cell walls, cytoplasmic membranes and mitochondrial membranes and destroying DNA to cause fungal cell death. However, if berberine is singly applied for a long time to prevent and treat the citrus lichen disease, the berberine is easy to generate drug resistance, so that the prevention effect of the berberine is reduced.
The research of the inventor finds that when berberine and sodium benzoate or sodium rosinate are compounded according to a certain mass ratio to prevent and treat citrus lichen diseases, obvious synergistic effect is shown, and no relevant report is found at present.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a composition containing berberine, which can improve the prevention and treatment of citrus lichen disease and delay the generation of drug resistance of pathogenic algae.
In order to achieve the purpose, the invention provides the following technical scheme:
the first purpose of the invention is to provide a composition containing berberine, the effective components of which are prepared by binary combination of berberine and sodium benzoate or sodium rosinate.
Preferably, the mass ratio of the berberine to the sodium benzoate is 1-10:40-1.
Preferably, the mass ratio of the berberine to the sodium rosinate is 1-5:15-1.
The second purpose of the invention is to provide the application of the composition in preventing and treating citrus moss.
Compared with the prior art, the invention has the following beneficial effects:
when the berberine is compounded with the sodium benzoate or the sodium rosinate, the compound preparation shows good synergistic effect on the unreal algae, can improve the control effect on the citrus lichen, can reduce the application dosage of pesticide, and delays the generation of drug resistance of pathogenic algae.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Pesticide effect in field: prevention and treatment effect on citrus lichen disease after berberine is compounded
1. Test agent
98% berberine crude drug (Shanghai Aladdin Biotechnology Co., ltd.), 99% sodium benzoate crude drug (Jiangxi Huayuyuan Biotechnology Co., ltd.), and 45% sodium rosinate wettable powder (Zhejiang Dapeng pharmaceutical Co., ltd.).
Preparing the raw medicines into single-dose mother liquor, setting multiple groups of ratios, and diluting each single-dose mother liquor and the mixture of the ratios with 0.1% Tween-80 until the mass concentration of the effective components is 30mg/L for later use.
2. The test method (refer to the pesticide field efficacy test rule (II) GB/T17980.98-2004: mango anthracnose prevention and control by bactericide).
In a Nanfeng mandarin orange orchard of Daliangchen town of Shangan county, liuzhou, guangxi, citrus trees with consistent growth vigor, plant depression and common citrus lichen disease are selected, and the pathogen is algae of Phaeococcus of Chlorophyta.
The experiment set up multiple treatments, 3 replicates each, 2 fruit trees each. And (3) spraying a test medicament on the whole plant of each treated citrus tree by adopting a spraying method in the occurrence period of the moss disease, uniformly spraying the front and back surfaces of the branches and the leaves of the citrus trees without dripping liquid medicine, and spraying equivalent clear water as a blank control.
The test was performed for 3 disease surveys: the 1 st investigation is the investigation before the first pesticide application, and the common disease of plants in a cell is investigated; the 2 nd investigation is 7d investigation after the first drug application; the 3 rd survey is 14d after the first administration. Each tree is investigated for 5 azimuths in the south, the west and the north, 10 leaves are investigated for each azimuth, the total leaf number and the leaf number of each grade of disease are recorded and investigated, and the disease index and the control effect of each treatment are calculated.
3. Blade disease grading table
See table 1.
TABLE 1 leaf disease grading Table
| Progression of disease | Lesion area of leaf |
| 0 | The leaf has no disease spot |
| 1 | The area of the scab accounts for less than 10% of the area of the leaf |
| 3 | The area of the scab accounts for 11-25% of the area of the leaf |
| 5 | The area of the lesion spots accounts for 26 to 50 percent of the area of the leaves |
| 7 | The area of the lesion spots accounts for 51-75% of the area of the leaves |
| 9 | The area of the scab accounts for more than 75 percent of the area of the leaf |
4. Formula for calculation
5. Test results
See tables 2-3.
TABLE 2 prevention and treatment effect of berberine and sodium benzoate combination on citrus lichen disease
As can be seen from Table 2, after the application of the drug for 7 days, the berberine, the sodium benzoate and different proportions of the berberine and the sodium benzoate gradually show certain control effects on the citrus lichen disease, and the control effect after the application of the drug for 14 days is better than that after the application of the drug for 7 days. When the medicine is applied for 14 days, the prevention effect of the berberine and sodium benzoate compound treatment is between 71.19 and 83.74 percent.
TABLE 3 prevention and treatment effect of berberine and sodium rosinate on citrus lichen disease
As can be seen from Table 3, after the application of the drug for 7 days, the berberine, the sodium rosinate and different proportions of the berberine and the sodium rosinate gradually show certain control effects on the citrus lichen diseases, and the control effect after the application of the drug for 14 days is better than that after the application of the drug for 7 days. When the medicine is applied for 14d, the prevention effect of the berberine and sodium rosinate after the compound treatment is between 68.84 and 86.93 percent, and the prevention effect of the berberine and the sodium rosinate after the single treatment is lower than 68.84 percent.
In conclusion, after the berberine is compounded with the sodium benzoate or the sodium rosinate, the synergistic effect is shown on the Phaeococcus pluvialis which is a pathogenic alga of the citrus moss disease within a certain mass ratio range, and the control effect on the citrus moss can be improved.
Claims (4)
1. A composition containing berberine is characterized in that the effective components of the composition are prepared by binary compounding of berberine and sodium benzoate or sodium rosinate.
2. The composition according to claim 1, wherein the weight ratio of berberine to sodium benzoate is 1-10:40-1.
3. The composition according to claim 1, wherein the mass ratio of berberine to sodium rosinate is 1-5:15-1.
4. Use of a composition according to any one of claims 1 to 3 for the control of citrus lichen disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211578920.2A CN115886016B (en) | 2022-12-07 | 2022-12-07 | Composition containing berberine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211578920.2A CN115886016B (en) | 2022-12-07 | 2022-12-07 | Composition containing berberine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115886016A true CN115886016A (en) | 2023-04-04 |
| CN115886016B CN115886016B (en) | 2024-04-30 |
Family
ID=86489503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211578920.2A Active CN115886016B (en) | 2022-12-07 | 2022-12-07 | Composition containing berberine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115886016B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961019A (en) * | 2010-10-29 | 2011-02-02 | 湖北太极生化开发有限公司 | Plant source bactericide and application thereof |
| CN102792975A (en) * | 2012-08-14 | 2012-11-28 | 安徽巨地农业科技有限公司 | Taste-type bird and mouse repellent composition |
| CN105994311A (en) * | 2016-06-22 | 2016-10-12 | 昆明百事德生物化学科技有限公司 | Botanical pesticide capable of controlling tobacco bacterial wilt and preparation method of botanical pesticide |
| CN111264523A (en) * | 2020-03-19 | 2020-06-12 | 成都新朝阳作物科学股份有限公司 | Application of DDAB in preparation of medicines for preventing and treating moss and moss preventing and treating composition |
| CN112715544A (en) * | 2021-01-27 | 2021-04-30 | 山东科太药业有限公司 | Pesticide composition for preventing and treating plant powdery mildew |
| CN114145305A (en) * | 2021-12-24 | 2022-03-08 | 广西壮族自治区农业科学院 | Composition for preventing and treating citrus moss |
-
2022
- 2022-12-07 CN CN202211578920.2A patent/CN115886016B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961019A (en) * | 2010-10-29 | 2011-02-02 | 湖北太极生化开发有限公司 | Plant source bactericide and application thereof |
| CN102792975A (en) * | 2012-08-14 | 2012-11-28 | 安徽巨地农业科技有限公司 | Taste-type bird and mouse repellent composition |
| CN105994311A (en) * | 2016-06-22 | 2016-10-12 | 昆明百事德生物化学科技有限公司 | Botanical pesticide capable of controlling tobacco bacterial wilt and preparation method of botanical pesticide |
| CN111264523A (en) * | 2020-03-19 | 2020-06-12 | 成都新朝阳作物科学股份有限公司 | Application of DDAB in preparation of medicines for preventing and treating moss and moss preventing and treating composition |
| CN112715544A (en) * | 2021-01-27 | 2021-04-30 | 山东科太药业有限公司 | Pesticide composition for preventing and treating plant powdery mildew |
| CN114145305A (en) * | 2021-12-24 | 2022-03-08 | 广西壮族自治区农业科学院 | Composition for preventing and treating citrus moss |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115886016B (en) | 2024-04-30 |
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