CN112715544A - Pesticide composition for preventing and treating plant powdery mildew - Google Patents

Pesticide composition for preventing and treating plant powdery mildew Download PDF

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CN112715544A
CN112715544A CN202110110108.6A CN202110110108A CN112715544A CN 112715544 A CN112715544 A CN 112715544A CN 202110110108 A CN202110110108 A CN 202110110108A CN 112715544 A CN112715544 A CN 112715544A
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powdery mildew
berberine
composition
methyl jasmonate
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宋晓军
谷大程
孙志锋
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Shandong Ketai Pharmaceutical Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system

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  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
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Abstract

The invention relates to a pesticide composition for preventing and treating plant powdery mildew, and belongs to the technical field of pesticides. The active ingredients in the composition are methyl jasmonate and berberine; the active component accounts for 1.5-15% of the composition, and the weight ratio of the methyl jasmonate to the berberine in the active component is 1: 12-12: 1. According to the invention, the methyl jasmonate and the berberine are compounded, so that the composition achieves a synergistic effect on preventing and treating plant powdery mildew.

Description

Pesticide composition for preventing and treating plant powdery mildew
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating plant powdery mildew.
Background
Methyl Jasmonate (MeJA), a fatty acid derivative with induced resistance, is commonly present in various plants, is an endogenous growth regulating substance present in higher plants, is also a signal molecule for pathogenic bacteria, elicitors and wound-induced plant defense gene expression, and can be used as a high-activity elicitor. A large amount of experimental data and molecular cell biology mechanisms disclose that methyl jasmonate as an important endogenous signal molecule participates in the defense of plants against pathogenic bacteria and phytophagous insects, generates jasmonic acid molecules on the surface and in vivo of the plants in a slow release manner and has strong induced stress resistance.
Berberine (Berberine) belongs to quaternary ammonium isoquinoline alkaloid, also known as Berberine. Mainly exists in berberidaceae, Papaveraceae, Ranunculaceae, Rutaceae and Menispermaceae plants. The berberine has a bacteriostatic mechanism, which is mainly characterized in that the permeability of an ion channel is changed, so that calcium ions of bacterial cells flow out to cause the destruction of the internal environment of bacteria, thereby inhibiting the growth of the bacteria. Meanwhile, the berberine can also inhibit the synthesis of macromolecular DNA, RNA and protein of bacterial cells, and the drug resistance of bacteria to the berberine is reduced through a multi-target bacteriostasis mechanism.
Powdery mildew is a disease with great harm to crops, once the crops are infected with the powdery mildew, the powdery mildew is fast to attack, repeatedly outbreaks and difficult to radically cure, and even the next crop is affected, so that serious consequences are caused. The existing chemical agents for preventing and treating powdery mildew have the problems of drug resistance, safety, low bactericidal activity, large dosage and the like, so that the production of crops is severely restricted, and the yield of the crops is reduced.
When berberine or methyl jasmonate is singly used for preventing and treating plant powdery mildew such as strawberry powdery mildew, melon powdery mildew, grape powdery mildew and the like, the problem of poor prevention and treatment effect exists. At present, the composition consisting of methyl jasmonate and berberine is not reported in the aspect of preventing and treating powdery mildew.
Disclosure of Invention
Aiming at the problems of low bactericidal activity, drug resistance, safety and the like in the prior art, the invention provides a pesticide composition for preventing and treating plant powdery mildew, and aims to solve the problems. The inventor finds that the methyl jasmonate and the berberine can be synergized, the drug resistance of powdery mildew is reduced by a multi-target bacteriostasis mechanism, the bactericidal activity is high, and the composition is a green and environment-friendly product, does not cause harm to the environment and human health, and is safe and nontoxic.
The technical scheme of the invention is as follows:
a pesticide composition for preventing and treating plant powdery mildew comprises methyl jasmonate and berberine as active ingredients; the active component accounts for 1.5-15% of the composition.
Preferably, the active component accounts for 5-6.5% of the composition.
Preferably, the weight ratio of the methyl jasmonate to the berberine in the active ingredients is 1: 12-12: 1.
Preferably, the weight ratio of the methyl jasmonate to the berberine in the active ingredients is 1: 1-12: 1.
Preferably, the pesticide composition also comprises auxiliary materials, wherein the auxiliary materials comprise the following components in percentage by weight: 0.5-5% of propylene glycol alginate, 0.1-0.8% of phytic acid, 0.1-1% of sodium benzoate and 10-40% of ethanol.
Preferably, the auxiliary materials comprise the following components in percentage by weight: 1-4% of propylene glycol alginate, 0.1-0.4% of phytic acid, 0.3-0.6% of sodium benzoate and 20-30% of ethanol. In the components, the propylene glycol alginate is used as a surfactant in the composition, the phytic acid can play a role in resisting oxidation, and the sodium benzoate can play a role in preserving.
The auxiliary materials are not limited to those provided by the above schemes, and all the auxiliary agents and fillers allowed in the pesticide can be mixed and matched.
A preparation method of a pesticide composition for preventing and treating plant powdery mildew comprises the following steps: dissolving methyl jasmonate in ethanol, adding berberine, phytic acid, sodium benzoate and propylene glycol alginate in sequence while stirring, finally supplementing the mixture to 100% with deionized water, and stirring and mixing uniformly to prepare the pesticide composition for preventing and treating powdery mildew.
The pesticide composition disclosed by the invention can be used for preventing and treating strawberry powdery mildew, melon powdery mildew and grape powdery mildew.
The invention has the beneficial effects that:
according to the invention, the methyl jasmonate and the berberine are compounded, so that the composition achieves a synergistic effect. In the invention, the methyl jasmonate is responsible for activating a jasmonic acid signal path, the methyl jasmonate and the berberine act on each part of the crop through the jasmonic acid path to activate an immune defense system in the crop or directly activate a crop defense gene, and the drug resistance of the powdery mildew is reduced by changing the permeability of a bacterial cell membrane, inhibiting the synthesis of bacterial genetic materials, activating a multi-target bacteriostasis mechanism of the crop defense system and the like, and the bactericidal activity is high. Meanwhile, the composition is a green and environment-friendly product, does not cause harm to the environment and human health, and is safe and non-toxic.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 indoor toxicity determination of methyl jasmonate, berberine and compound combination thereof on strawberry powdery mildew
The toxicity of the medicament on strawberry powdery mildew is determined by adopting a hypha growth rate method according to 'indoor pesticide bioassay test standard bactericide'. And after 72h, measuring and recording the colony diameter of each treatment by adopting a cross method, and calculating the net growth amount and the hypha growth inhibition rate of each treatment. Converting the hypha growth inhibition rate into a probability value (y), converting the drug solution concentration (mg/L) into a logarithm value (x), obtaining a virulence regression equation (y is a + bx) by a least square method, and calculating the EC of each drug50Value, actually measured virulence index (ATI), theoretical virulence index (TTI). Meanwhile, the synergistic effect of the mixed medicaments is evaluated according to the co-toxicity coefficient (CTC) method of the Sun Yunpei method, namely the CTC is less than or equal to 80, the composition shows antagonistic effect, and when the 80 is equal to 80<CTC<120, the composition shows additive effect, and when CTC is more than or equal to 120, the composition shows synergistic effect. The calculation formula is as follows, and the test results are shown in Table 1.
Figure BDA0002918908340000031
Theoretical virulence factor (TTI) ═ A virulence index of the agent x percent of A in the mixture + B virulence index of the agent x percent of B in the mixture
Figure BDA0002918908340000032
TABLE 1 toxicity test results analysis table of methyl jasmonate, berberine and their combination for strawberry powdery mildew
Figure BDA0002918908340000033
As can be seen from Table 1, EC of methyl jasmonate and berberine on strawberry powdery mildew50The values are 12.39mg/L and 27.84mg/L respectively, when the compounding ratio of the methyl jasmonate to the berberine is 12:1-1:12, the co-toxicity coefficient (CTC) is more than 120, and good synergistic effect is shown, wherein the compounding ratio is 1: 1-12: 1, and the synergistic effect is more obvious.
Example 2 indoor toxicity assay of methyl jasmonate, berberine and their combination against powdery mildew of melon
The toxicity of the medicament on strawberry powdery mildew is determined by adopting a hypha growth rate method according to 'indoor pesticide bioassay test standard bactericide'. And after 72h, measuring and recording the colony diameter of each treatment by adopting a cross method, and calculating the net growth amount and the hypha growth inhibition rate of each treatment. Converting the hypha growth inhibition rate into a probability value (y), converting the drug solution concentration (mg/L) into a logarithm value (x), obtaining a virulence regression equation (y is a + bx) by a least square method, and calculating the EC of each drug50Value, actually measured virulence index (ATI), theoretical virulence index (TTI). Meanwhile, the synergistic effect of the mixed medicaments is evaluated according to the co-toxicity coefficient (CTC) method of the Sun Yunpei method, namely the CTC is less than or equal to 80, the composition shows antagonistic effect, and when the 80 is equal to 80<CTC<120, the composition shows additive effect, and when CTC is more than or equal to 120, the composition shows synergistic effect. The calculation formula is as follows, and the test results are shown in Table 2.
Figure BDA0002918908340000041
Theoretical virulence factor (TTI) ═ A virulence index of the agent x percent of A in the mixture + B virulence index of the agent x percent of B in the mixture
Figure BDA0002918908340000042
TABLE 2 toxicity test results analysis table of methyl jasmonate, berberine and their combination for melon powdery mildew
Figure BDA0002918908340000043
As can be seen from Table 2, the EC of methyl jasmonate and berberine on powdery mildew of melon50The values are 15.46mg/L and 30.25mg/L respectively, when the compounding ratio of the methyl jasmonate to the berberine is 12:1-1:12, the co-toxicity coefficient (CTC) is more than 120, and good synergistic effect is shown, wherein the synergistic effect is more obvious when the compounding ratio is 1: 1-12: 1.
Example 3 indoor toxicity determination of methyl jasmonate, berberine and their combination on grape powdery mildew
The toxicity of the medicament on the grape powdery mildew is determined by adopting a hypha growth rate method according to 'indoor pesticide bioassay test standard bactericide'. And after 72h, measuring and recording the colony diameter of each treatment by adopting a cross method, and calculating the net growth amount and the hypha growth inhibition rate of each treatment. Converting the hypha growth inhibition rate into a probability value (y), converting the drug solution concentration (mg/L) into a logarithm value (x), obtaining a virulence regression equation (y is a + bx) by a least square method, and calculating the EC of each drug50Value, actually measured virulence index (ATI), theoretical virulence index (TTI). Meanwhile, the synergistic effect of the mixed medicaments is evaluated according to the co-toxicity coefficient (CTC) method of the Sun Yunpei method, namely the CTC is less than or equal to 80, the composition shows antagonistic effect, and when the 80 is equal to 80<CTC<120, the composition shows additive effect, and when CTC is more than or equal to 120, the composition shows synergistic effect. The calculation formula is as follows, and the test results are shown in Table 3.
Figure BDA0002918908340000044
Theoretical virulence factor (TTI) ═ A virulence index of the agent x percent of A in the mixture + B virulence index of the agent x percent of B in the mixture
Figure BDA0002918908340000051
TABLE 3 toxicity test results analysis table of methyl jasmonate, berberine and their combination for grape powdery mildew
Figure BDA0002918908340000052
As can be seen from Table 3, the EC of methyl jasmonate and berberine on grape powdery mildew50The values are 14.37mg/L and 29.53mg/L respectively, the co-toxicity coefficient (CTC) is more than 120 when the compounding ratio of the methyl jasmonate to the berberine is 12:1-1:12, the co-toxicity coefficient (CTC) is 12:1-1:12, and the good synergistic effect is shown, wherein the compounding ratio is 1: 1-12: 1, and the synergistic effect is more obvious.
Example 4
A pesticide composition for preventing and treating plant powdery mildew comprises 3% of methyl jasmonate, 3% of berberine, 4% of propylene glycol alginate, 0.1% of phytic acid, 0.6% of sodium benzoate and 30% of ethanol. Dissolving methyl jasmonate in ethanol, adding berberine, phytic acid, sodium benzoate and propylene glycol alginate in sequence while stirring, and finally supplementing deionized water to 100%, stirring and mixing uniformly to obtain the pesticide composition.
Example 5
A pesticide composition for preventing and treating plant powdery mildew comprises 4% of methyl jasmonate, 2% of berberine, 3% of propylene glycol alginate, 0.2% of phytic acid, 0.5% of sodium benzoate and 25% of ethanol. Dissolving methyl jasmonate in ethanol, adding berberine, phytic acid, sodium benzoate and propylene glycol alginate in sequence while stirring, and finally supplementing deionized water to 100%, stirring and mixing uniformly to obtain the pesticide composition.
Example 6
A pesticide composition for preventing and treating plant powdery mildew comprises methyl jasmonate 6%, berberine 0.5%, propylene glycol alginate 1%, phytic acid 0.4%, sodium benzoate 0.3%, and ethanol 20%. Dissolving methyl jasmonate in ethanol, adding berberine, phytic acid, sodium benzoate and propylene glycol alginate in sequence while stirring, and finally supplementing deionized water to 100%, stirring and mixing uniformly to obtain the pesticide composition.
Example 7
A pesticide composition for preventing and treating plant powdery mildew comprises 5% of methyl jasmonate, 1% of berberine, 2% of propylene glycol alginate, 0.3% of phytic acid, 0.4% of sodium benzoate and 25% of ethanol. Dissolving methyl jasmonate in ethanol, adding berberine, phytic acid, sodium benzoate and propylene glycol alginate in sequence while stirring, and finally supplementing deionized water to 100%, stirring and mixing uniformly to obtain the pesticide composition.
Application example 1
The Weifang Anqiu strawberry greenhouse is used as a test base, and the following experimental design is carried out to verify the effect of preventing and treating powdery mildew;
treatment 1.1: blank control (clear water spraying control)
Treatment 1.2: example 4 dilution of sample 400-fold spray application
Treatment 1.3: example 5 dilution of sample 400-fold spray application
Treatment 1.4: example 6 dilution of sample 400-fold spray application
Treatment 1.5: example 7 dilution of sample 400-fold spray application
Treatment 1.6: 6% methyl jasmonate single-agent sample diluted 400 times and sprayed
Treatment 1.7: 2% berberine single-agent sample diluted 400 times for spraying
Spraying the pesticide from the early stage of powdery mildew of the strawberries, spraying the pesticide for 1 time every 7 days, continuously spraying the pesticide for 3 times, counting disease indexes before the pesticide, and investigating and controlling effects 7 days after the first pesticide spraying, 7 days after the second pesticide spraying and 7 days after the third pesticide spraying respectively. And (4) carrying out grading investigation on the occurrence of powdery mildew of strawberries. And randomly taking 5 regions for each treatment, selecting 5 strawberries (marked) with uniform growth and disease incidence conditions for each region, and counting the disease spots of all leaves, wherein each leaf is recorded in a grading manner according to the percentage of the disease spots in the leaf area. Grading the disease condition: level 0: no disease spots; level 1: the lesion area accounts for less than 5% of the whole leaf area; and 3, level: the lesion area accounts for 6 to 10 percent of the whole leaf area; and 5, stage: the lesion area accounts for 11 to 20 percent of the whole leaf area; and 7, stage: the lesion area accounts for 21-40% of the whole leaf area; and 9, stage: the disease spot area accounts for more than 40% of the whole leaf area, the disease index and the prevention and treatment effect are calculated in the following way, and the statistical results are shown in table 4:
Figure BDA0002918908340000061
Figure BDA0002918908340000062
TABLE 4 prevention and treatment effect of three-time pesticide spraying in strawberry greenhouse on powdery mildew
Figure BDA0002918908340000071
As can be seen from the test results in Table 4, the control effects of the 4 samples of the examples 7 days after the first spraying, 7 days after the second spraying and applying and 7 days after the third spraying and applying are better than those of the single methyl jasmonate agent, the single berberine agent and the blank treatment, and the maximum control effect is 90.22% after the third spraying. Therefore, the composition of methyl jasmonate and berberine can be used for preventing and treating strawberry powdery mildew in a synergistic manner.
Application example 2
Taking the Zhen melon greenhouse in Shen county Yan shop as a test base, carrying out the following experimental design to verify the effect of preventing and treating powdery mildew;
treatment 2.1: blank control (clear water spraying control)
Treatment 2.2: example 4 dilution of sample 400-fold spray application
Treatment 2.3: example 5 dilution of sample 400-fold spray application
Treatment 2.4: example 6 dilution of sample 400-fold spray application
Treatment 2.5: example 7 dilution of sample 400-fold spray application
Treatment 2.6: 6% methyl jasmonate single-agent sample diluted 400 times and sprayed
Treatment 2.7: 2% berberine single-agent sample diluted 400 times for spraying
Spraying the pesticide from the early stage of powdery mildew of melons, spraying the pesticide for 1 time every 7 days, continuously spraying the pesticide for 3 times, counting disease indexes before the pesticide, and investigating and controlling effects 7 days after spraying the pesticide for the first time, 7 days after spraying the pesticide for the second time and 7 days after spraying the pesticide for the third time. And (4) carrying out grading investigation on the occurrence condition of powdery mildew of melons. And randomly taking 5 regions for each treatment, selecting 3 melons with uniform growth and disease incidence conditions (marked by red ropes) in each region, and counting disease spots of all leaves, wherein each leaf is recorded according to the percentage of the disease spots in the leaf area in a grading manner. Grading the disease condition: level 0: no disease spots; level 1: the lesion area accounts for less than 5% of the whole leaf area; and 3, level: the lesion area accounts for 6 to 10 percent of the whole leaf area; and 5, stage: the lesion area accounts for 11 to 20 percent of the whole leaf area; and 7, stage: the lesion area accounts for 21-40% of the whole leaf area; and 9, stage: the disease area of the lesion is more than 40 percent of the whole leaf area, the disease index and the prevention and treatment effect are calculated in the following way, and the statistical result is shown in table 5.
Figure BDA0002918908340000081
Figure BDA0002918908340000082
TABLE 5 preventing and treating effect of three-time pesticide spraying on powdery mildew in melon greenhouse
Figure BDA0002918908340000083
As can be seen from the test results in Table 5, the control effects of the 4 samples of the examples 7 days after the first spraying, 7 days after the second spraying and applying and 7 days after the third spraying and applying are better than those of the single methyl jasmonate agent, the single berberine agent and the blank treatment, and the maximum control effect is 88.05% after the third spraying. Therefore, the composition of the methyl jasmonate and the berberine can prevent and control powdery mildew of melons in a synergistic manner.
Application example 3
The method is characterized in that a Zhongping Changshan grape plantation is used as a test base, and the following experimental design is carried out to verify the effect of preventing and treating powdery mildew;
treatment 3.1: blank control (clear water spraying control)
Treatment 3.2: example 4 dilution of sample 400-fold spray application
Treatment 3.3: example 5 dilution of sample 400-fold spray application
Treatment 3.4: example 6 dilution of sample 400-fold spray application
Treatment 3.5: example 7 dilution of sample 400-fold spray application
Treatment 3.6: 6% methyl jasmonate single-agent sample diluted 400 times and sprayed
Treatment 3.7: 2% berberine single-agent sample diluted 400 times for spraying
Spraying the pesticide from the early stage of grape powdery mildew, spraying the pesticide for 1 time every 7 days, continuously spraying the pesticide for 3 times, counting disease indexes before the pesticide, and investigating and controlling effects 7 days after the first pesticide spraying, 7 days after the second pesticide spraying and 7 days after the third pesticide spraying respectively. And (4) carrying out grading investigation on the occurrence of grape powdery mildew. And randomly taking 5 regions for each treatment, selecting 3 grapes (marked) with uniform growth and disease incidence conditions for each region, and counting the disease spots of all leaves, wherein each leaf is recorded in a grading manner according to the percentage of the disease spots in the leaf area. Grading the disease condition: level 0: no disease spots; level 1: the lesion area accounts for less than 5% of the whole leaf area; and 3, level: the lesion area accounts for 6 to 10 percent of the whole leaf area; and 5, stage: the lesion area accounts for 11 to 20 percent of the whole leaf area; and 7, stage: the lesion area accounts for 21-40% of the whole leaf area; and 9, stage: the disease area accounts for more than 40% of the whole leaf area, the disease index and the prevention and treatment effect are calculated in the following way, and the statistical results are shown in table 3.
Figure BDA0002918908340000091
Figure BDA0002918908340000092
TABLE 6 prevention and treatment effect of three-time pesticide spraying in grape plantation on powdery mildew
Figure BDA0002918908340000093
As can be seen from the test results in Table 6, the control effects of the 4 samples of the examples 7 days after the first spraying, 7 days after the second spraying and applying and 7 days after the third spraying and applying are better than those of the single methyl jasmonate agent, the single berberine agent and the blank treatment, and the maximum control effect is 86.42% after the third spraying. Therefore, the composition of methyl jasmonate and berberine can prevent and treat grape powdery mildew in a synergistic manner.
Although the present invention has been described in detail by way of preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made on the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and these modifications or substitutions are within the scope of the present invention/any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (8)

1. A pesticide composition for preventing and treating plant powdery mildew is characterized in that the active ingredients in the composition are methyl jasmonate and berberine; the active component accounts for 1.5-15% of the composition.
2. The pesticide composition for controlling plant powdery mildew according to claim 1, wherein the active component accounts for 5-6.5% of the composition.
3. The pesticide composition for preventing and treating plant powdery mildew according to claim 1, wherein the weight ratio of methyl jasmonate to berberine in the active ingredients is 1: 12-12: 1.
4. The pesticide composition for preventing and treating plant powdery mildew according to claim 1, wherein the weight ratio of methyl jasmonate to berberine in the active ingredients is 1: 1-12: 1.
5. The pesticide composition for controlling plant powdery mildew according to claim 1, characterized by further comprising auxiliary materials, wherein the auxiliary materials comprise the following components in percentage by weight: 0.5-5% of propylene glycol alginate, 0.1-0.8% of phytic acid, 0.1-1% of sodium benzoate and 10-40% of ethanol.
6. The pesticide composition for controlling plant powdery mildew according to claim 1, wherein the auxiliary materials comprise the following components in percentage by weight: 1-4% of propylene glycol alginate, 0.1-0.4% of phytic acid, 0.3-0.6% of sodium benzoate and 20-30% of ethanol.
7. A process for the preparation of a pesticidal composition according to any one of claims 1 to 6, characterized in that it comprises: dissolving methyl jasmonate in ethanol, adding berberine, phytic acid, sodium benzoate and propylene glycol alginate in sequence while stirring, finally supplementing the mixture to 100% with deionized water, and stirring and mixing uniformly to prepare the pesticide composition for preventing and treating powdery mildew.
8. Use of a pesticidal composition according to any one of claims 1 to 6 for controlling plant powdery mildew including strawberry powdery mildew, melon powdery mildew and grape powdery mildew.
CN202110110108.6A 2021-01-27 2021-01-27 Pesticide composition for preventing and treating plant powdery mildew Pending CN112715544A (en)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN115886016A (en) * 2022-12-07 2023-04-04 广西壮族自治区农业科学院 A composition containing berberine

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CN101986841A (en) * 2009-07-30 2011-03-23 罗门哈斯公司 Synergistic microbicidal compositions
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