CN116548454A - Formula and application of sterilization composition containing benziothiazolinone - Google Patents

Formula and application of sterilization composition containing benziothiazolinone Download PDF

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CN116548454A
CN116548454A CN202211242881.9A CN202211242881A CN116548454A CN 116548454 A CN116548454 A CN 116548454A CN 202211242881 A CN202211242881 A CN 202211242881A CN 116548454 A CN116548454 A CN 116548454A
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benziothiazolinone
treatment
copper
formulation
bactericidal composition
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张通
曹巧利
冯塔
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Shaanxi Meibang Pharmaceutical Group Co ltd
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Shaanxi Meibang Pharmaceutical Group Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A01N25/14—Powders or granules wettable
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A01N43/42—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/20—Copper
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • General Health & Medical Sciences (AREA)
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Abstract

The invention relates to a formulation of a sterilization composition containing benziothiazolinone and application thereof, in particular to a formulation of a sterilization composition containing benziothiazolinone and copper quinolinate, wherein the sterilization composition comprises the following components in parts by weight: 5% -20% of benziothiazolinone, 10% -45% of copper quinolinate, 1% -8% of a synergistic agent, 1% -10% of a dispersing agent, 1% -10% of a wetting agent, 0.5% -20% of a binding agent, 0% -2% of a defoaming agent, 0% -2% of a pH regulator and 100% of a filler, wherein the weight ratio of the benziothiazolinone to the copper quinolinate is 1:2-1:4, and the sterilization composition is prepared into water dispersible granules. The bactericidal composition has good control effect on bacterial angular leaf spot of muskmelon, is safe to crops and is environment-friendly. The dosage of the effective active ingredients is 75-150 g/hectare when the bactericidal composition is used for preventing and treating bacterial angular leaf spot of muskmelon.

Description

Formula and application of sterilization composition containing benziothiazolinone
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a formulation of a sterilization composition containing benziothiazolinone and application thereof.
Background
The benziothiazolinone is a novel broad-spectrum bactericide, and is mainly used for preventing and treating various bacterial and fungal diseases such as cucumber downy mildew, pear scab, apple scab, citrus anthracnose, grape anthracnose and the like. The sterilization mechanism mainly comprises two aspects of destroying the nuclear structure of germ cells, leading the germ cells to lose heart parts and failure death and interfering metabolism of germ cells, leading the germ cells to be physiological disorder and finally leading the germ cells to death.
The quinoline copper is a quinoline protective low-toxicity bactericide, belongs to organic copper chelate, has broad spectrum, high efficiency, low residue and safe use, has good prevention and treatment effects on fungal and bacterial diseases, and particularly has good prevention and treatment effects on pathogenic bacteria with drug resistance. Forming a layer of tight protective medicine film on the surface of the plant after spraying, and having strong affinity with the plant and rain wash resistance; the medicinal film slowly releases sterilized copper ions, and effectively inhibits the germination and invasion of germs, thereby achieving the purpose of preventing and curing diseases.
Experiments show that the water dispersible granule composition prepared from the benziothiazolinone and the copper quinolinolate with different action mechanisms according to a certain proportion can effectively improve the control effect on melon bacterial angular leaf spot, reduce the control cost and have high safety on crops.
Disclosure of Invention
The invention aims to provide a formulation of a sterilization composition containing benziothiazolinone and application thereof, and provides an application of the sterilization composition in preventing and treating melon bacterial angular leaf spot.
The invention solves the technical difficulties as follows: the prior art has few medicaments for preventing or controlling the bacterial angular leaf spot of muskmelon, mainly antibiotics and copper preparation pesticides, and the antibiotics pesticides have strong drug resistance because of frequent use, and the copper preparation is easy to burn leaves and generate phytotoxicity in the use process.
The technical scheme of the invention is as follows:
the formulation and application of the bactericidal composition containing the benziothiazolinone are characterized in that: the bactericidal composition comprises the following components in parts by weight: 5 to 20 percent of benziothiazolinone, 10 to 45 percent of copper quinolinate, 1 to 8 percent of synergistic agent, 1 to 10 percent of dispersing agent, 0.5 to 10 percent of wetting agent, 1 to 20 percent of binder, 0 to 2 percent of defoamer, 0 to 2 percent of PH regulator and 100 percent of filler.
Preferably, the bactericidal composition comprises the following components in parts by weight: 10% of benziothiazolinone, 40% of copper quinolinate, 2% to 6% of synergistic agent, 2% to 6% of dispersing agent, 2% to 6% of wetting agent, 1% to 10% of binder, 0.1% to 0.8% of defoamer, 0% to 1% of PH regulator and 100% of filler.
The synergist is selected from one of polyethoxy modified silane and polyethoxy modified fatty alcohol mixture or alkoxy modified polysilicone;
the dispersing agent is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, methylene dinaphthyl sodium sulfonate, lignin sodium salt or calcium salt, melamine formaldehyde resin, alkyl naphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphoric acid, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, dodecyl polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate, nonylphenol polyoxyethylene ether phosphate, naphthalene sulfonic formaldehyde condensate sodium salt block copolymer, comb-type polycarboxylate and sodium polycarboxylate;
the wetting agent is one or more selected from sodium dodecyl benzene sulfonate, sodium diisobutyl naphthalene sulfonate, sorbitan fatty acid ester, sulfodocyl sodium salt, alkyl succinic acid sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, triphenyl ethylphenol polyoxypropylene polyoxyethylene block polymer, sodium dodecyl sulfate, styrene phenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside and fatty alcohol polyoxyethylene ether sodium sulfonate;
the binder is one or more selected from water, polyvinylpyrrolidone, polyethylene glycol, glucose, polyvinyl alcohol and starch;
the PH regulator is one or more selected from light calcium, glacial acetic acid, citric acid and potassium dihydrogen phosphate;
the defoamer is one or more selected from organic silicone, fatty alcohol and fatty acid;
the filler is selected from the group consisting of: white carbon black, calcined kaolin, ammonium sulfate, potassium sulfate, magnesium sulfate, sodium sulfate, talcum powder, diatomite, ammonium chloride, urea and starch.
Preferably, the dosage form of the bactericidal composition is water dispersible granule.
Preferably, the weight ratio of the benziothiazolinone to the copper quinolinate is 1:2-1:4, and more preferably the weight ratio of the benziothiazolinone to the copper quinolinate is 1:4.
Preferably, the total weight of the benziothiazolinone and the copper quinolinate in the bactericidal composition is 20% -65%, and more preferably the total weight of the benziothiazolinone and the copper quinolinate is 50%.
Preferably, the use of the bactericidal composition for controlling bacterial angular leaf spot of melon.
Preferably, the bactericidal composition is used for preventing and treating bacterial angular leaf spot of muskmelon, the dosage of the effective components is 75-150 g/hectare, and more preferably, the dosage of the effective components is 112.5-150 g/hectare.
Preferably, the fungicidal composition is used to control bacterial angular leaf spot of melon with a water usage of 450 to 750 litres per hectare, more preferably with a water usage of 750 litres per hectare.
Compared with the prior art, the bactericidal composition for preventing and treating the bacterial angular leaf spot of the muskmelon has the following beneficial effects: (1) Compared with a single dose, the composition has more remarkable effect on preventing and treating bacterial angular leaf spot of muskmelon; (2) The compound with two different action mechanisms is mixed, so that the generation of drug resistance of diseases can be effectively delayed, the safety is good, and the compound is safe to melons; (3) Has good sterilization effect, quick action and lasting effect, and the lasting period can reach more than 15 days.
Detailed Description
The invention is further illustrated by the following examples, which are by weight, but are not limited thereto.
Application example one
Example 1 50% Thiomolone-quinoline copper Water dispersible granule
The 50% benziothiazolinone-quinoline copper water dispersible granule is prepared by adding 95% benziothiazolinone original drug by 10%, 98% quinoline copper original drug by 40%, 3% of a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 5% of white carbon black, 3% of methyl naphthalene sulfonate formaldehyde condensate, 2% of EO-PO block polyether, 3% of sodium dodecyl benzene sulfonate, 3% of alkyl glycoside, 3% of polyethylene glycol, 0.2% of an organosilicone defoamer, 0.1% of citric acid and 100% of starch.
Example 2 50% Thiomolone-quinoline copper Water dispersible granule
The 50% benziothiazolinone-quinoline copper water dispersible granule is prepared by adding 95% benziothiazolinone original drug by 10%, 98% quinoline copper original drug by 40%, alkoxy modified polysilicone by 4%, potassium sulfate by 6%, alkylphenol ethoxylate formaldehyde condensate sulfate by 3%, sodium polycarboxylate by 4%, diisobutyl naphthalene sulfonate by 3%, sorbitan fatty acid ester by 3%, polyvinylpyrrolidone by 3%, organic silicone defoamer by 0.2%, starch by 15% and diatomite by 100%.
Example 3 25% Thiomolone-quinoline copper Water dispersible granule
95% of benziothiazolinone technical material, 20% of 98% of oxine copper technical material, 5% of alkoxy modified polysilicone, 5% of ammonium chloride, 5% of lignin sodium salt or calcium salt, 4% of castor oil polyoxyethylene ether, 2% of polyethylene glycol, 0.2% of glacial acetic acid, 0.3% of fatty alcohol, and 100% of calcined kaolin and sodium sulfate to prepare 25% of benziothiazolinone-oxine copper water dispersible granules.
Example 4 40% Thiomolone-quinoline copper Water dispersible granule
The water dispersible granule is prepared from the raw materials of 95% of benziothiazolinone, 98% of copper quinoline, 32% of the raw materials of the copper quinoline, 4% of a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 6% of white carbon black, 4% of styrene phenol polyoxyethylene ether phosphate, 3% of sodium polycarboxylate, 3% of fatty alcohol polyoxyethylene ether, 2% of glucose, 8% of starch, 0.4% of fatty acid and 100% of potassium sulfate.
Example 5 60% Thiomolone-quinoline copper Water dispersible granule
95% of a benziothiazolinone original drug, 20% of a 98% of a quinoline copper original drug, 40% of an alkoxy modified polysilicone, 2% of a naphthalene sulfonic acid formaldehyde condensate sodium salt block copolymer, 3% of fatty alcohol polyoxyethylene ether phosphate, 2% of sulfosuccinyl octyl ester sodium salt, 2% of a triphenyl ethylphenol polyoxypropylene polyoxyethylene block polymer, 1% of polyvinyl alcohol, 1% of water, 0.2% of an organic silicone defoamer and 100% of starch to prepare 60% of the benziothiazolinone quinoline copper water dispersible granule.
Implementation example II:
determination of toxicity of mixing of benziothiazolinone and copper quinoline on melon bacterial angular leaf spot
According to the standards of NY/T1156.16-2008 and NY/T1156.6-2006, the toxicity of the benziothiazolinone and the copper quinolinate and the mixing ratio of 1:1, 1:2, 1:4, 4:1 and 2:1 to the melon bacterial angular leaf spot bacteria is measured by adopting a turbidity method, and the result shows that the benziothiazolinone and the copper quinolinate are mixed according to the 5 ratios, and the synergism is shown for the melon bacterial angular leaf spot bacteria. The experimental procedure was as follows:
1 purpose of test
The toxicity of the benziothiazolinone and the copper quinolinate and the mixed combination of the benziothiazolinone and the copper quinolinate with different proportions on the bacterial angular leaf spot bacteria of muskmelon is measured indoors, the synergism is evaluated, the suitability of the benziothiazolinone and the copper quinolinate is clear, and a scientific basis is provided for the research and development of the benziothiazolinone and copper quinolinate mixture.
2 test conditions
2.1 test targets
The melon bacterial angular leaf spot germ is stored and provided by Hunan loving seedling detection limited company.
2.2 culture conditions
The culture condition of the target to be tested and the target after the test is 28-30 ℃.
2.3 instrumentation
Beaker, pipette, triangular flask, autoclave, constant temperature shake incubator, ultraviolet spectrophotometer, etc.
3 test design
3.1 test Agents
Benziothiazolinone (benzodiazinone) 95% active ingredient and copper quinoline (copper-quinoline) 98% active ingredient.
3.2 design of concentration of agent and formulation of solution
Weighing 0.1000g of a 95% benziothiazolinone original drug by using a sterilized small beaker, dissolving the original drug by using 1mL of DMF, adding 189mL of sterile water containing 0.1% Tween80 emulsifier to prepare 500mg/L mother liquor, and diluting the mother liquor into 250, 125, 62.5, 31.25 and 15.625mg/L of sterile water containing 0.1% Tween80 emulsifier for standby;
weighing 0.1000g of 98% quinoline copper raw medicine by using a sterilized small beaker, dissolving the raw medicine by using 1mL of DMF, adding 195mL of sterile water containing 0.1% Tween80 emulsifier to prepare 500mg/L mother liquor, and diluting the mother liquor into 250, 125, 62.5, 31.25 and 15.625mg/L of sterile water containing 0.1% Tween80 emulsifier for standby;
respectively adding 25, 20, 10, 40 and 40mL of 500mg/L benziothiazolinone solution into 25, 40, 10 and 20mL of 500mg/L copper quinolinate solution to prepare 500mg/L benziothiazolinone copper quinolinate 1:1, 1:2, 1:4, 4:1 and 2:1 solutions, and diluting with sterile water containing 0.1% Tween80 emulsifier to prepare 250, 125, 62.5, 31.25 and 15.625mg/L solutions for later use;
control solution 1mL DMF was added 99mL sterile water containing 0.1% Tween80 emulsifier.
4 test method
Referring to the standard method of biological measurement NY/T1156.16-2008, a turbidity method is adopted, namely 5mL of each single dose of liquid medicine with serial concentration is taken and added into 45mL of NB culture medium cooled to 45 ℃ to prepare the drug-containing culture medium with required final concentration. Diluting the strain grown on the slant of NA medium with sterilized water to 1×10 7 Each treatment medium was inoculated with 100ul of the bacterial suspension at a concentration of spores/mL, and each treatment was repeated 4 times. After the treatment, the mixture is placed in a shaking culture (120 r/min) at the temperature of 28-30 ℃ for 12 hours, the absorbance is measured, and the growth inhibition rate is calculated.
5 data investigation and statistical analysis
5.1 investigation method
The absorbance of each treatment was measured before the incubation was started, and when the control treatment reached the logarithmic growth phase, the absorbance of each treatment was measured and recorded. The wavelength measured was 660nm. Growth inhibition (%) was calculated.
Wherein: p represents the growth inhibition rate; a is that 0 Indicating the increase value of the blank control absorbance value; a is that 1 The absorbance increase value of the chemical treatment is shown.
5.2 method for evaluating synergy
The synergy of the mixed medicines is evaluated according to a co-toxicity coefficient method (CTC) of Sun & Johnson (1960) by referring to a biological assay criterion NY/T1156.6-2006 in a pesticide room, namely CTC is less than or equal to 80 and is antagonistic, CTC is 80 and CTC is 120 and is additive, and CTC is more than or equal to 120 and is synergistic.
Drug combination Theory Toxicity Index (TTI) =toxicity index of agent a x percent (%) of agent a in the combination + percent (%)
5.3 statistical analysis of data
All experimental data were analyzed using DPS v9.50 statistical software. Bacterial growth inhibition ((%) was calculated from the test data and the virulence regression equation, correlation coefficient (r) and EC were calculated 50 (95% confidence limit).
6 results analysis and discussion
6.1 evaluation of drug efficacy
The toxicity measurement results of the mixing combination of the benziothiazolinone and the copper quinolinolate in different proportions on melon bacterial angular leaf spot bacteria are shown in table 1. As can be seen from Table 1, in the 1:1, 1:2, 1:4, 4:1, 2:1 combinations of benziothiazolinone and copper quinolinate, five ratios exhibited synergistic effects on melon bacterial angular leaf spot bacteria, and their EC 50 The values are respectively7.25, 7.22, 6.45, 8.64, 7.76mgL, co-toxicity coefficients 137.95, 143.42, 162.77, 122.72, 137.52, respectively. Wherein the synergistic effect of the two components is most obvious when the two components are mixed in a ratio of 1:4.
TABLE 1 toxicity determination results of the combination of benziothiazolinone and copper quinoline on melon bacterial angular leaf spot
6.2 discussion and conclusion
Indoor biological test results show that the synergistic effect of the benziothiazolinone and the copper quinolinate is shown by mixing the benziothiazolinone and the copper quinolinate in the proportion of 1:1, 1:2, 1:4, 4:1 and 2:1, wherein the synergistic effect of the benziothiazolinone and the copper quinolinate is most obvious by mixing the benziothiazolinone and the copper quinolinate in the proportion of 1:4, so that the optimal mixing ratio of the copper quinolinate and the copper quinolinate can be seen by 1:4.
Application example three: example 1 field efficacy experiment for controlling bacterial angular leaf spot of melon
Standard operation procedure of field efficacy test for controlling bacterial angular leaf spot of crops such as vegetables and fruits by Bactericide (KCJH-SOP 01-127) of Chengjia agricultural technology Co., ltd.
Test crop melon
Bacterial angular leaf spot of prevention and treatment object
1 test basic information
1.1 test name
50% of the benziothiazolinone-quinoline copper water dispersible granule is used for field efficacy test of melon bacterial angular leaf spot.
1.2 purpose of test
The 50% benziothiazolinone-quinoline copper water dispersible granule provided by the applicant has the effects of preventing and treating bacterial angular leaf spot of muskmelon, is applicable to dosage and safety, and provides basis for registering and popularizing test medicaments.
2 environmental and facility cultivation conditions
2.1 test position
Zhuang Zhen in taan city, shandong province.
2.2 test target conditions
Melon bacterial angular leaf spot (Pseudomonas syringae)
2.3 testing crops, varieties and growth
Melon is of a kind of green precious stone, and is in a melon saving period during drug application, and has good growth vigor.
3 test design and arrangement
3.1 test amount and numbering
TABLE 2 test design of reagents for test
In order to ensure that experiments are carried out smoothly, the dosage of the actual medicament is increased by 10 percent compared with the theoretical value.
3.2 methods of administering drugs
3.2.1 periods of application and methods
The first application time is 2019, 8 and 2; the second application time is 2019, 8, 9; the third application time is 2019, 8, 16; the drug was co-administered 3 times.
3.2.2 usage Capacity
Spraying stems and leaves, and using 750 liters of liquid medicine per hectare (50L/mu).
3.3 investigation method, time and times
3.3.1 investigation time and times
The total leaf number and the leaf number of each stage were investigated 4 times before administration, 7 days after 1 st administration, 7 days after 2 nd administration and 10 days after 3 rd administration, namely 8 months 2 days in 2019, 9 days in 8 months, 16 days in 8 months and 26 days in 8 months.
3.3.2 investigation method
Sampling at 5 points on the diagonal of each cell, 3 plants at each point, investigating the disease condition of all leaves, and grading according to the area percentage of the disease spots of each leaf. The blade classification method comprises the following steps:
grade 0, no disease spots;
stage 1: the area of the disease spots accounts for less than 5% of the whole leaf area;
stage 3, the area of the lesion accounts for 6% -10% of the whole leaf area;
stage 5, the area of the lesion accounts for 11% -20% of the whole leaf area;
7, the area of the lesion accounts for 21-50% of the whole leaf area;
stage 9: the area of the disease spots accounts for more than 51% of the whole leaf area.
3.3.3 method for calculating efficacy
Note that CK 0 、CK 1 The disease indexes before and after the administration of the drug in the blank control area are respectively shown; PT (PT) 0 、PT 1 The disease index before and after the drug administration in the drug treatment area is respectively.
4 results and analysis
Table 3 results of field efficacy test of 50% benziothiazolinone quinoline copper Water dispersible granule of example 1 for controlling melon bacterial angular leaf spot
The test results show (Table 3) that the 50% oxine-benziothiazolinone water dispersible granule of the test object has better control effect on melon bacterial angular leaf spot, and the control effect shows an increasing trend along with the increase of the dosage and the dosage times.
The specific analysis shows that the average control effects of the tested object 50% of the oxine-benziothiazolinone water dispersible granule treatment A (the dosage of the preparation is 10 g/mu), the treatment B (the dosage of the preparation is 15 g/mu), the treatment C (the dosage of the preparation is 20 g/mu) are 63.59%, 68.56% and 72.02% in sequence, the average control effects of the control object 33.5% of the oxine-copper suspending agent treatment D (the dosage of the preparation is 60 g/mu), the 3% of the benziothiazolinone microemulsion treatment E (the dosage of the preparation is 90 g/mu) and the average control effects of the test object 46% of the copper hydroxide water dispersible granule treatment F (the dosage of the preparation is 60 g/mu) are 67.43%, 66.60% and 65.01% in sequence, wherein the average control effect of the treatment C is the highest, and the control effect is obvious from other treatment differences. Treatment a was significantly different from treatment B, C, D, E and not significantly different from treatment F; treatment B differed significantly from treatment C, E, F, but not from treatment D; treatment D differed significantly from treatment F, but not significantly from treatment E; treatment E differed significantly (0.05 level) from treatment F.
The average control effects of the tested substances 50% of the oxine-benziothiazolinone water dispersible granule treatment A, the treatment B and the treatment C are 70.62%, 75.32% and 78.64% in sequence, the average control effects of the control substance 33.5% of the oxine-copper suspending agent treatment D, the 3% of the benziothiazolinone microemulsion treatment 5 and the 46% of the copper hydroxide water dispersible granule treatment F are 74.84%, 73.81% and 72.29% in sequence, wherein the average control effect of the treatment C is the highest, and the average control effects are obviously different from other treatments. Treatment a differed significantly from treatment B, C, D, E, but not from treatment F; treatment B differed significantly from treatment C, F, but not from treatment E, and not from treatment D; treatment D differed from treatment E but not significantly, from treatment F; treatment E was different from treatment F but not significant (0.05 level).
The average control effects of 50% of the test object, namely the oxine-benziothiazolinone water dispersible granule, in treatment A, treatment B and treatment C are 75.40%, 81.04% and 84.56% in sequence, the average control effects of 33.5% of the control object, namely the oxine-copper suspending agent, in treatment D, 3% of the benziothiazolinone microemulsion, in treatment 5 and 46% of the copper hydroxide water dispersible granule, in treatment F, are 80.22%, 78.51% and 77.59% in sequence, wherein the average control effect of treatment C is the highest, and the average control effects are remarkably different from other treatments. Treatment a differed significantly from treatment B, C, D, E, F; treatment B differed significantly from treatment C, E, F and not from treatment D; treatment D differed significantly from treatment E, F; treatment E did not differ significantly from treatment F (0.05 level).
Application example four: EXAMPLE 2 test of efficacy against bacterial angular leaf spot of melon
Reference is made to the third application example for specific experimental basis, experimental agent and dosage, water consumption and calculation method.
1 test position
The Shandong province Weifang city is the longevity city.
2 test crop, variety and growth conditions
Melon is of the type of honey sheep horn, and is in the vine stretching stage when being applied. The growth is good.
3 method of administering a drug
3.1 periods of application and methods
The test was applied 3 times. The administration time is 9 months 12 days, 9 months 19 days and 9 months 26 days in 2020.
3.3 investigation time
Investigation was performed before the first administration, 7 days after the second administration, and 10 days after the third administration, i.e., 9 months 12 days, 9 months 19 days, 9 months 26 days, and 10 months 6 days in 2020, for a total of 4 times.
3.4 investigation method
Sampling 5 points on the diagonal of each cell, 3 strains at each point, investigating all the leaves, and grading the leaves according to the percentage of the disease spot area of each leaf to the whole leaf area:
the blade classification method comprises the following steps:
grade 0, no disease spots;
stage 1: the area of the disease spots accounts for less than 5% of the whole leaf area;
stage 3, the area of the lesion accounts for 6% -10% of the whole leaf area;
stage 5, the area of the lesion accounts for 11% -20% of the whole leaf area;
7, the area of the lesion accounts for 21-50% of the whole leaf area;
stage 9: the area of the disease spots accounts for more than 51% of the whole leaf area.
4 results and analysis
Table 4 test results of 50% benziothiazolinone quinoline copper water dispersible granule of example 2 for preventing and treating melon bacterial angular leaf spot
The test results show (Table 4) that the 50% quinolyl copper-benziothiazolinone water dispersible granule of the test object in the embodiment 2 has a good control effect on melon bacterial angular leaf spot, and the control effect tends to be improved along with the increase of the dosage and the dosage times.
The specific analysis shows that the average control effects of the tested object 50% of the oxine-benziothiazolinone water dispersible granule treatment A (the dosage of the preparation is 10 g/mu), the treatment B (the dosage of the preparation is 15 g/mu), the treatment C (the dosage of the preparation is 20 g/mu) are 65.65%, 70.56% and 75.31% in sequence, the average control effect of the control object 33.5% of the oxine-copper suspending agent treatment D (the dosage of the preparation is 60 g/mu), the control object 3% of the benziothiazolinone microemulsion treatment E (the dosage of the preparation is 90 g/mu) and the control object 46% of the copper hydroxide water dispersible granule treatment F (the dosage of the preparation is 60 g/mu) are 69.79%, 67.96% and 67.16% in sequence, wherein the average control effect of the treatment C is the highest, and the average control effect is obvious from other treatments. Treatment B and treatment D did not differ significantly, treatment E and treatment F did not differ significantly, and the difference between the other treatments was significant (0.05 level).
The average control effects of the tested object treatment A, the treatment B and the treatment C are 72.37%, 77.52% and 80.54% in sequence 7 days after the second application, and the average control effects of the control object treatment D, the treatment E and the treatment F are 75.50%, 73.98% and 75.80% in sequence, wherein the average control effect of the treatment C is highest, and the treatment is obviously different from other treatments. Treatment D and treatment F were not significantly different, and the remaining treatments were significantly different from each other (0.05 level).
The average control effect of the test object treatment A, the test object treatment B and the test object treatment C is 76.43%, 82.30% and 85.66% in sequence, and the average control effect of the control object treatment D, the control object treatment E and the control object treatment F is 81.38%, 80.42% and 81.46% in sequence, wherein the average control effect of the test object treatment C is highest. Treatment B differed from treatment D, F but not significantly, treatment D from treatment G did not significantly, treatment E differed from treatment D, F but not significantly, and the remaining differences between treatments were significant (0.05 level).
The observation in the test shows that the 50% quinolyl copper-benziothiazolinone water dispersible granule of the embodiment 2 has no obvious influence on melon results, leaf colors and plant growth states in the test dosage range, and is safe to melons and harmless to the melons.
The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. It will be apparent to those skilled in the art that several simple deductions or substitutions may be made without departing from the spirit of the invention, and these should be considered to be within the scope of the invention.

Claims (9)

1. The formulation and application of the bactericidal composition containing the benziothiazolinone are characterized in that: the bactericidal composition comprises the following components in parts by weight: 5-20% of benziothiazolinone, 10-45% of copper quinolinate, 1-8% of a synergistic agent, 1-10% of a dispersing agent, 0.5-10% of a wetting agent, 1-20% of a binding agent, 0-2% of a defoaming agent, 0-2% of a PH regulator and 100% of a filler.
2. The bactericidal composition formulation of claim 1 wherein: the bactericidal composition comprises the following components in parts by weight: 10% of benziothiazolinone, 40% of copper quinolinate, 2% -6% of a synergistic agent, 2% -6% of a dispersing agent, 2% -6% of a wetting agent, 1% -10% of a binder, 0.1% -0.8% of a defoaming agent, 0% -1% of a pH regulator and 100% of a filler.
3. A formulation of a fungicidal composition according to claim 1 or 2, characterized in that: the dosage form of the bactericidal composition is water dispersible granule.
4. A formulation of a fungicidal composition according to claim 3, wherein: the weight ratio of the benziothiazolinone to the copper quinolinate is 1:2-1:4.
5. The bactericidal composition formulation of claim 4 wherein: the weight ratio of the benziothiazolinone to the copper quinolinate is 1:4.
6. A formulation of a fungicidal composition according to claim 4 or 5, wherein: the total weight of the benziothiazolinone and the copper quinolinolate in the bactericidal composition is 20% -65%.
7. The bactericidal composition formulation of claim 6 wherein: the total weight of the benziothiazolinone and the copper quinolinolate in the bactericidal composition is 50 percent.
8. A formulation of a fungicidal composition and its use as claimed in claim 1, wherein: the bactericidal composition is used for preventing and treating bacterial angular leaf spot of muskmelon.
9. The bactericidal composition formulation and use as recited in claim 8 wherein: the dosage of the effective components of the bactericidal composition for preventing and treating the bacterial angular leaf spot of muskmelon is 75-150 g/hectare.
CN202211242881.9A 2022-10-11 2022-10-11 Formula and application of sterilization composition containing benziothiazolinone Pending CN116548454A (en)

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