CN102369938B - Sterilization composition containing zineb and chunleimeisu - Google Patents
Sterilization composition containing zineb and chunleimeisu Download PDFInfo
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- CN102369938B CN102369938B CN 201010258313 CN201010258313A CN102369938B CN 102369938 B CN102369938 B CN 102369938B CN 201010258313 CN201010258313 CN 201010258313 CN 201010258313 A CN201010258313 A CN 201010258313A CN 102369938 B CN102369938 B CN 102369938B
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- zineb
- kasugarnycin
- chunleimeisu
- sterilization
- leaf
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- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 title claims abstract description 76
- 239000011717 all-trans-retinol Substances 0.000 title claims abstract description 38
- 235000019169 all-trans-retinol Nutrition 0.000 title claims abstract description 38
- 239000000203 mixture Substances 0.000 title claims abstract description 31
- 230000001954 sterilising effect Effects 0.000 title abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 title abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 13
- 230000000844 anti-bacterial effect Effects 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 16
- 238000009736 wetting Methods 0.000 claims description 10
- 238000009472 formulation Methods 0.000 claims description 3
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- 239000000825 pharmaceutical preparation Substances 0.000 claims 1
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- 239000000654 additive Substances 0.000 abstract 1
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- 239000003814 drug Substances 0.000 description 31
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- 239000003795 chemical substances by application Substances 0.000 description 15
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- 238000011835 investigation Methods 0.000 description 11
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- UFNOUKDBUJZYDE-UHFFFAOYSA-N 2-(4-chlorophenyl)-3-cyclopropyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol Chemical compound C1=NC=NN1CC(O)(C=1C=CC(Cl)=CC=1)C(C)C1CC1 UFNOUKDBUJZYDE-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
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- 244000144730 Amygdalus persica Species 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 239000005757 Cyproconazole Substances 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
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- 229920001732 Lignosulfonate Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000220324 Pyrus Species 0.000 description 1
- 240000003377 Shepherdia canadensis Species 0.000 description 1
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 241000317942 Venturia <ichneumonid wasp> Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
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- 238000012271 agricultural production Methods 0.000 description 1
- 150000004996 alkyl benzenes Chemical class 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
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- 239000003899 bactericide agent Substances 0.000 description 1
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- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 229940077388 benzenesulfonate Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 1
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- 230000002950 deficient Effects 0.000 description 1
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- 230000017858 demethylation Effects 0.000 description 1
- 238000010520 demethylation reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- NVVZQXQBYZPMLJ-UHFFFAOYSA-N formaldehyde;naphthalene-1-sulfonic acid Chemical compound O=C.C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 NVVZQXQBYZPMLJ-UHFFFAOYSA-N 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229910052625 palygorskite Inorganic materials 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229920005646 polycarboxylate Polymers 0.000 description 1
- 229920000056 polyoxyethylene ether Polymers 0.000 description 1
- 229940051841 polyoxyethylene ether Drugs 0.000 description 1
- 229940088417 precipitated calcium carbonate Drugs 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
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- 150000003839 salts Chemical class 0.000 description 1
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- 159000000000 sodium salts Chemical class 0.000 description 1
- PYODKQIVQIVELM-UHFFFAOYSA-M sodium;2,3-bis(2-methylpropyl)naphthalene-1-sulfonate Chemical compound [Na+].C1=CC=C2C(S([O-])(=O)=O)=C(CC(C)C)C(CC(C)C)=CC2=C1 PYODKQIVQIVELM-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
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- 238000012546 transfer Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses a sterilization composition containing zineb and chunleimeisu, a preparation method and an application thereof. The sterilization composition is characterized in that: the sterilization composition comprises the zineb, the chunleimeisu and other additives, wherein a weight ratio of the zineb to the chunleimeisu is 100:1-1:2. The sterilization composition can be applicable for prevention of diseases caused by a plurality of crop fungi. The sterilization combination has characteristics of safety, environmental protection, low cost and good effect. Field efficacy test results show that: the sterilization combination has a significant synergistic effect. The combination of the zineb and the chunleimeisu is not reported at home and abroad. Therefore, the sterilization composition of the present invention is provided.
Description
Technical field
The present invention relates to a kind of agricultural bactericidal composition, refer in particular to a kind of bactericidal composition and application thereof that contains zineb and kasugarnycin.
Background technology
Use chemical agent to prevent and treat in agricultural production, producing resistance is inevitably, and has the research and development of the noval chemical compound of desinsection, bactericidal activity, and cost height, cycle are long, does not especially catch up with the speed of disease generation resistance.Adopting mechanism of action difference, composite two kinds of compounds with synergistic effect to carry out composite is to select preferably.
Zineb
Zineb is the protectiveness organosulfureous fungicide.Pure product are pale powder, and industrial goods are canescence or pale yellow powder, and sulfur-containing aroma is arranged.Unstable under alkalescence, high temperature, humidity, day illumination solarization condition.To people and animals' low toxicity, but people's skin, nose, throat there is spread effect.White powder, 157 ℃ of decomposition, no fusing point.Vapour pressure<0.01mPa (20 ℃).Solvability is 10mg/L in the room temperature water, is insoluble to most of organic solvents, but can be dissolved in pyridine.To light, heat, moisture instability, easily decompose, meet the material of alkaline matter or cupric, mercury, also easily decompose.More than the rat acute per os toxicity LD50 5200mg/kg.
Kasugarnycin:
Be disinfectant use in agriculture, the rice blast on the paddy rice is had excellent preventive effect and therapeutic action.In rice ear sprouting period and pustulation period dispenser, to solid nothing influence.When control rice blast, chestnut seasonal febrile diseases, working concentration is 40r, as use the 6000r/g wetting powder, every 50g medicinal powder adds water 75kg, sprays about 1 mu, spray medicine when the leaf pest reach 2 grades, should spray once about 7 days after the dispenser first time when being in a bad way, the control panicle blast sprays when going out fringe 1/3rd left and right sides in the rice field, when panicle blast is serious again, except the dispenser of cut phase, full heading time also will spray medicine one time.The dispenser of can directly dusting of 0.4% pulvis, every mu of consumption 1.5kg is preferably in dispenser when sooner or later dew being arranged, and medicinal powder can be sticked in the rice strain.In the test of having found, kasugarnycin is prevented and treated 20 milliliters of water flasks of cladosporium leaf and fruit mould of tomato, control watermelon bacterial angular leaf spot, and peach gummosis, shot hole, diseases such as shothole disease have special efficacy.
Summary of the invention
The objective of the invention is, overcome the defective that zineb and the agent of kasugarnycin list produce resistance problem, a kind of bactericidal composition that contains zineb and kasugarnycin that provides a kind of delaying drug resistance to produce, reduce consumption, improve the lasting period, reduce production costs.
Measure of the present invention realizes by following measures.
A kind of bactericidal composition, it contains zineb and kasugarnycin, and wherein the weight ratio of zineb and kasugarnycin is 100: 1~1: 2.
Described bactericidal composition, wherein the more excellent weight ratio of zineb and kasugarnycin is 80: 1~1: 1.
Described bactericidal composition, wherein to account for the percentage of preparation total amount be 5.5~75% to the accumulating weight of zineb and kasugarnycin.
Described bactericidal composition, zineb and kasugarnycin and auxiliary agent, carrier etc. are processed into any one formulation that allows on the agricultural chemicals.
Described bactericidal composition is characterized in that the formulation that can be processed into is wetting powder.
The application of described bactericidal composition aspect the multiple diseases of control cereal class and fruit tree, melon, vegetables etc.
Specific embodiments of the present invention is as follows:
Described bactericidal composition is wetting powder, and the percentage by weight of component is:
Zineb 5~50%
Kasugarnycin 0.5~40%
Dispersant 3~20%
Wetting agent 3~10%
Filler 10~70%
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with zineb and kasugarnycin and dispersant, wetting agent and filler mixing, in stirred tank, evenly stir, behind airslide disintegrating mill, mixing, namely can be made into the wetting powder of the present composition.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, alkylphenol polyoxyethylene formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, Nekal BX., among the wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
The invention has the beneficial effects as follows:
1, two kinds of compound structures involved in the present invention, through evidence, being mixed mutually can not produce conflict, but Synergistic reduces dosage, reduces cost.And zineb and kasugarnycin composite do not have report as yet.
2, the pathogen that produces resistance is still had very high drug effect, but suspension of pesticide resistance generation.
3, reduce the agricultural chemicals usage amount, strengthened the friendly to environment.
4, reduce the agricultural chemicals usage amount, also reduced drug cost, strengthened the market competitiveness of this prescription.
Embodiment: zineb and kasugarnycin composite
Further the present invention is made an explanation by following examples, but scope of the present invention is not limited to following examples.
1 materials and methods
1.1 test material
1.1.1 reagent agent
The former medicine of 90% zineb, Hebei Shuangji chemical limited company produces; The former medicine of 55% kasugarnycin, Jilin Province prolongs limit spring thunder Bioceuticals Inc. and produces;
1.1.2 for the examination pathogen
The cladosporium leaf and fruit mould of tomato bacterium is gathered the cladosporium leaf and fruit mould of tomato leaf from Shouguang City's green house, adopts tissue isolation to the pathogen separation and purification, is cultivating preservation on the PSA slant medium in 4 ℃ of refrigerators.
1.1.3 for the examination medium
PSA medium: potato 200g, sucrose 20g, agar 20g, water 1000ml
1.2 test method and synergistic effect are measured
1.2.1 two kinds of single agent suppress the toxicity test of tomato leaf mycete mycelial growth
The former medicinal an amount of pyridine dissolving of zineb is made into 1000 μ g/ml mother liquors, and is stand-by.Kasugarnycin dissolves with sterile water, and is stand-by.
On superclean bench, mother liquor is diluted to 5~6 concentration gradients, accurately pipetting the 1mL soup joins in the P D A medium that melts about 9mL50 ℃, after mixing, pour into rapidly in advance in the diameter 9cm culture dish of sterilization, the cooling back the culture medium flat plate of pastille.
Cut the bacterium cake of the silk that carries disease germs with the diameter 5mm card punch of sterilization at the cladosporium leaf and fruit mould of tomato bacterium colony edge of cultivating 10 days, with transfer needle it is inserted on pastille culture medium flat plate, each culture dish is placed 1 bacterium cake.Each is handled and repeats 3 times.In 25 ℃ of insulating boxs, cultivate 11d, adopt the right-angled intersection method to measure colony diameter, get its mean value, calculate growth inhibition ratio.
Bacteriostasis rate (%)=((the clean amount of growth of the contrast bacterium colony-clean amount of growth of chemicals treatment bacterium colony)/clean amount of growth of contrast bacterium colony) * 100
Clean amount of growth=the colony diameter of bacterium colony-bacterium cake diameter
Concentration logarithm value with reagent agent is independent variable, is dependent variable with the relative inhibition probit value, carries out the data analysis statistics with the DPS data handling system, obtains virulence regression equation, EC
50Value and correlation coefficient.
On the basis of preliminary experiment, respectively single agent zineb and kasugarnycin are carried out toxicity test in order to last method, the two EC
50Value is respectively 15.6 μ g/ml and 2.8 μ g/ml.
Combination arranges 1.2.2 be mixed
It is 48.65%+1.35%, 48%+2%, 46.2%+3.8%, 42.8%+7.2% and 37.5%+12.5% totally 5 combinations that are mixed that 50% zineb kasugarnycin wetting powder mass ratio is respectively 36: 1,24: 1,12: 1,6: 1 and 3: 1.
1.2.3 mixture toxicity test
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC
50/ reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A of TII=A in using with
CTC=(ATI/TTI)×100
If co-toxicity coefficient is big by 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100~120, be indicated as summation action
Data see Table 1
The different proportioning zineb of table 1 kasugarnycin mixture is to virulence and the synergistic effect of cladosporium leaf and fruit mould of tomato bacterium
The composite co-toxicity to the cladosporium leaf and fruit mould of tomato bacterium of table 2 zineb kasugarnycin
2 results and analysis
It is 136.9 that zineb and kasugarnycin are mixed at 24: 1 o'clock SR, shows as synergistic effect, and obviously greater than 120, all the other show as summation action.Comprehensive each side factor thinks that it is best proportioning that zineb and kasugarnycin were mixed 24: 1.
3 field trials are measured the control efficiency to cladosporium leaf and fruit mould of tomato and early blight of tomato
3.1 field trial is measured the control efficiency to cladosporium leaf and fruit mould of tomato
3.1.1 application method
Test is total to dispenser 3 times, check plot spray equivalent clear water.When making up a prescription, after with low amounts of water medicament fully being dissolved earlier, add suitable quantity of water again and carry out complete stool spraying processing, even spraying.All adopting power driven sprayer, operating pressure during the spray medicine is 2.0~2.3kg/cm
2, shower nozzle aperture 1.2mm.The residential quarter spouting liquid is used by 675 liters/hectare, and soup is sprayed application to the positive and negative two sides of crop leaf uniformly.With the moistening blade of soup, not following is degree.
3.1.2 investigation method
Every residential quarter is got 5 investigation at random, looks into 3 strains at every, and 5 on leaf is got in every strain upper, middle and lower, records total number of sheets, the sick number of sheets at different levels, calculates control efficiency.
Stage division is as follows:
0 grade: no scab;
1 grade, lesion area accounts for below 5% of whole leaf area;
3 grades, lesion area accounts for 10% of whole leaf area;
5 grades, lesion area accounts for 11%~20% of whole leaf area;
7 grades, lesion area accounts for 21%~50% of whole leaf area;
9 grades, lesion area accounts for more than 50% of whole leaf area.
3.1.3 control time and number of times
Investigate i.e. disease index investigation before the medicine, preventive effect investigation in 7 days behind the 1st medicine, 7 days preventive effect investigation behind the 2nd medicine altogether 3 times.
3.1.4 drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
3.1.5 the direct influence to crop.
Whether have poisoning produce, will record poisoning degree and type if any poisoning if observing crop.In addition, also should record other useful influences to crop.
3.1.6 result of the test and analysis
Each handles the effect of preventing and treating cladosporium leaf and fruit mould of tomato table 3
Interpretation of result:
As can be seen from Table 3, to prevent and treat the effect of cladosporium leaf and fruit mould of tomato more remarkable in the combination that is mixed of 50% zineb kasugarnycin wetting powder 24: 1.Under the consumption of same every mu 20 gram, the effect of complex preparation significantly is better than two kinds of single agent, and is the most outstanding with lasting effect.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.7 days control efficiency reaches 74.14% and 80.94% respectively behind the 1st, 2 medicine.And single agent zineb and kasugarnycin 7 days the highest preventive effect behind the 2nd medicine is respectively 69.24% and 75.86%, significantly inferior to complex preparation.Result of the test proves that complex preparation has significant synergistic effect under rational proportion, and the development of effectively having controlled the main disease of tomato is caused harm, and has very important meaning in production practices.According to the field range estimation, in the test dose scope, tomato growth is normal, and each is handled medicament and the poisoning phenomenon all do not occur.
3.2 field trial is measured the control efficiency to early blight of tomato
3.2.1 application method
Test is total to dispenser three times, check plot spray equivalent clear water.When making up a prescription, after with low amounts of water medicament fully being dissolved earlier, add suitable quantity of water again and carry out complete stool spraying processing, even spraying.All adopting power driven sprayer, operating pressure during the spray medicine is 2.0~2.3kg/cm
2, shower nozzle aperture 1.2mm.The residential quarter spouting liquid is used by 675 liters/hectare, and soup is sprayed application to the positive and negative two sides of crop leaf uniformly.With the moistening blade of soup, not following is degree.
3.2.2 investigation method
Take five point samplings, the investigation of fixing a point, every residential quarter random searching 5 points, 2 strains of every some investigation tomato, every strain branch upper, middle and lower are investigated 10 blades respectively for 3 layers, record total number of sheets and the sick number of sheets at different levels; Investigate the whole fruits of this 10 strain tomato simultaneously, record total fruit number and sick fruit number at different levels.
Sick leaf (or sick fruit) grade scale:
0 grade: no scab;
1 grade: lesion area accounts for whole blade (really) area below 5.0%;
3 grades: lesion area accounts for whole blade (really) area 5.1%~10.0%;
5 grades: lesion area accounts for whole blade (really) area 10.1%-20.0%;
7 grades: lesion area accounts for whole blade (really) area 20.1%-50.0%;
9 grades: lesion area accounts for whole blade (really) area more than 50.1%.
3.2.3 control time and number of times
Test is investigated 4 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 14 days preventive effect investigation behind 10 days and the 3rd (last) medicine behind the 2nd medicine.
3.2.4 drug effect computational methods
Sick leaf rate (%)=sick leaf (really) count/is investigated total leaf (really) number * 100
Disease index=∑ (sick leaves at different levels (really) number * relative level numerical value)/(investigating total leaf (really) number * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
3.2.5 the direct influence to crop.
Whether have poisoning produce, will record poisoning degree and type if any poisoning if observing crop.
3.2.6 result of the test and analysis
Each handles the effect of preventing and treating early blight of tomato table 4
Interpretation of result:
As can be seen from Table 4, the effect of 24: 1 the black star of combination control pears that is mixed of 50% zineb kasugarnycin wetting powder is remarkable.Under the consumption of same every mu 20 gram, the effect of complex preparation significantly is better than two kinds of single agent.Complex preparation significantly is better than effect under the low concentration consumption in control efficiency under the consumption of high concentration.14 days lasting effect is more remarkable after 10 days control efficiency behind the 1st, 2 medicine reaches 76.31% and 84.10%, the 3 medicine respectively, reaches 87.46%.And the highest preventive effect of single agent zineb and kasugarnycin is respectively 76.57% and 80.04%, significantly inferior to complex preparation.
In sum, the present invention contains the sterilization combination of zineb and kasugarnycin, cladosporium leaf and fruit mould of tomato and early blight of tomato are all had good control efficiency, and it is to the target crop safety.Containing cyproconazole in the complex preparation, is sterol demethylation inhibitor, has preventive and therapeutic action, to belong to the mould genus of spore, the Venturia bacterium is all effective.Zineb then is broad spectrum activity protection type bactericide, and is all effective to most of fungies.Complex preparation is had outstanding performance in test, has not only effectively suppressed further developing of germ, and through checking, has enlarged fungicidal spectrum, has delayed the drug-fast generation of germ.Comprehensive every index shows that complex preparation significantly is better than single agent, so research and development of the present invention and popularization have very important meaning to society.
Claims (2)
1. bactericidal composition that contains zineb and kasugarnycin, it is characterized in that: the active ingredient of this bactericidal composition is zineb and kasugarnycin binary built, and wherein the mass ratio of active ingredient zineb and kasugarnycin is that 24:1 and zineb and the gross weight of kasugarnycin in preparation account for 50% of the whole quality of the pharmaceutical preparations.
2. bactericidal composition according to claim 1, it is characterized in that: the formulation of this bactericidal composition is wetting powder.
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US3358001A (en) * | 1963-12-28 | 1967-12-12 | Umezawa Hamao | Antibiotic kasugamycin |
CN1234179A (en) * | 1998-04-17 | 1999-11-10 | 罗姆和哈斯公司 | Improved dithiocarbamate bactericide composition |
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US3358001A (en) * | 1963-12-28 | 1967-12-12 | Umezawa Hamao | Antibiotic kasugamycin |
CN1234179A (en) * | 1998-04-17 | 1999-11-10 | 罗姆和哈斯公司 | Improved dithiocarbamate bactericide composition |
Non-Patent Citations (2)
Title |
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DETECTION OF ERWINIA AMYLOVORA STRAINS WITH REDUCED SENSITIVITY TO CURRENTLY REGISTERED ANTIBIOTICS AND MANAGEMENT OF FIRE BLIGHT OF POME FRUITS USING KASUGAMYCIN IN INTEGRATED STRATEGIES;J.E.Adaskaveg, et al.;《ISHS Acta horticulturae 793: XI International Workshop on Fire Blight》;20080630;第1卷;375-382 * |
J.E.Adaskaveg, et al..DETECTION OF ERWINIA AMYLOVORA STRAINS WITH REDUCED SENSITIVITY TO CURRENTLY REGISTERED ANTIBIOTICS AND MANAGEMENT OF FIRE BLIGHT OF POME FRUITS USING KASUGAMYCIN IN INTEGRATED STRATEGIES.《ISHS Acta horticulturae 793: XI International Workshop on Fire Blight》.2008,第1卷375-382. |
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