CN102369938A - Sterilization composition containing zineb and chunleimeisu - Google Patents
Sterilization composition containing zineb and chunleimeisu Download PDFInfo
- Publication number
- CN102369938A CN102369938A CN2010102583139A CN201010258313A CN102369938A CN 102369938 A CN102369938 A CN 102369938A CN 2010102583139 A CN2010102583139 A CN 2010102583139A CN 201010258313 A CN201010258313 A CN 201010258313A CN 102369938 A CN102369938 A CN 102369938A
- Authority
- CN
- China
- Prior art keywords
- zineb
- kasugarnycin
- chunleimeisu
- sterilization
- leaf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses a sterilization composition containing zineb and chunleimeisu, a preparation method and an application thereof. The sterilization composition is characterized in that: the sterilization composition comprises the zineb, the chunleimeisu and other additives, wherein a weight ratio of the zineb to the chunleimeisu is 100:1-1:2. The sterilization composition can be applicable for prevention of diseases caused by a plurality of crop fungi. The sterilization combination has characteristics of safety, environmental protection, low cost and good effect. Field efficacy test results show that: the sterilization combination has a significant synergistic effect. The combination of the zineb and the chunleimeisu is not reported at home and abroad. Therefore, the sterilization composition of the present invention is provided.
Description
Technical field
The present invention relates to a kind of agricultural bactericidal composition, refer in particular to a kind of bactericidal composition and application thereof that contains zineb and kasugarnycin.
Background technology
In agricultural production, use chemical agent to prevent and treat, producing resistance is inevitably, and has the research and development of the noval chemical compound of desinsection, bactericidal activity, and cost is high, the cycle is long, does not especially catch up with the speed that disease produces resistance.Adopting mechanism of action difference, composite two kinds of compounds with synergistic effect to carry out composite is to select preferably.
Zineb
Zineb is the protectiveness organosulfureous fungicide.Pure article are pale powder, and industrial goods are canescence or pale yellow powder, and sulfur-containing aroma is arranged.Unstable under alkalescence, high temperature, humidity, day illumination solarization condition.To people and animals' low toxicity, but people's skin, nose, throat there is spread effect.White powder, 157 ℃ of decomposition, no fusing point.Vapour pressure＜0.01mPa (20 ℃).Solvability is 10mg/L in the room temperature water, is insoluble to most of organic solvents, but can be dissolved in pyridine.To light, heat, moisture instability, be prone to decompose, meet the material of alkaline matter or cupric, mercury, also be prone to decomposition.More than the rat acute per os toxicity LD50 5200mg/kg.
Kasugarnycin:
Be disinfectant use in agriculture, the rice blast on the paddy rice is had excellent preventive effect and therapeutic action.In rice ear sprouting period and pustulation period dispenser, to solid nothing influence.When control rice blast, chestnut seasonal febrile diseases, working concentration is 40r, as uses the 6000r/g wetting powder; Every 50g medicinal powder adds water 75kg, sprays about 1 mu spray medicine when the leaf pest reach 2 grades; Should spray once about 7 days after the dispenser first time when being in a bad way, the control panicle blast sprays when going out fringe 1/3rd left and right sides in the rice field, when panicle blast is serious again; Except that the dispenser of cut phase, full heading time also will spray medicine one time.The dispenser of can directly dusting of 0.4% pulvis, every mu of consumption 1.5kg is preferably in dispenser when sooner or later dew being arranged, and medicinal powder can be sticked in the rice strain.In the test of having found, kasugarnycin is prevented and treated 20 milliliters of water flasks of cladosporium leaf and fruit mould of tomato, control watermelon bacterial angular leaf spot, and peach gummosis, shot hole, diseases such as shothole disease have special efficacy.
Summary of the invention
The objective of the invention is, overcome the defective that zineb and the agent of kasugarnycin list produce resistance problem, a kind of bactericidal composition that contains zineb and kasugarnycin that provides a kind of delaying drug resistance to produce, reduce consumption, improve the lasting period, reduce production costs.
Measure of the present invention realizes through following measures.
A kind of bactericidal composition, it contains zineb and kasugarnycin, and wherein the weight ratio of zineb and kasugarnycin is 100: 1～1: 2.
Described bactericidal composition, wherein the more excellent weight ratio of zineb and kasugarnycin is 80: 1～1: 1.
Described bactericidal composition, wherein to account for the percentage of preparation total amount be 5.5～75% to the accumulating weight of zineb and kasugarnycin.
Described bactericidal composition, zineb and kasugarnycin and auxiliary agent, carrier etc. are processed into any one formulation that allows on the agricultural chemicals.
Described bactericidal composition is characterized in that the formulation that can be processed into is a wetting powder.
The application of described bactericidal composition aspect the multiple diseases of control cereal class and fruit tree, melon, vegetables etc.
Specific embodiments of the present invention is following:
Described bactericidal composition is a wetting powder, and the percentage by weight of component is:
Zineb 5～50%
Kasugarnycin 0.5～40%
Dispersant 3～20%
Wetting agent 3～10%
Filler 10～70%
The concrete procedure of processing of this bactericidal composition wetting powder is: press above-mentioned prescription with zineb and kasugarnycin and dispersant, wetting agent and filler mixing; In stirred tank, evenly stir; Behind airslide disintegrating mill, mixing, promptly can be made into the wetting powder of the present composition.
Described dispersant is selected from one or more in polycarboxylate, lignosulfonates, APES formaldehyde condensation products sulphate, alkyl benzene sulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, APES, aliphatic amine polyoxyethylene ether, polyoxyethylene carboxylate, the fatty acid glyceride APEO.
Described wetting agent is selected from: lauryl sodium sulfate, calcium dodecyl benzene sulfonate, Nekal BX., among the wetting and penetrating agent F, alkylnaphthalene sulfonate, polyoxyethylene triphenylethylene phosphenylic acid salt, spaonin powder, silkworm excrement, soapberry powder one or more.
Described filler is selected from: one or more in kaolin, diatomite, bentonite, attapulgite, white carbon, starch, the precipitated calcium carbonate.
The invention has the beneficial effects as follows:
1, two kinds of compound structures involved in the present invention, through evidence, being mixed each other can not produce conflict, but Synergistic reduces dosage, reduces cost.And zineb and kasugarnycin composite do not have report as yet.
2, the pathogen that produces resistance is still had very high drug effect, but suspension of pesticide resistance generation.
3, reduced the agricultural chemicals usage amount, strengthened friendly environment.
4, reduce the agricultural chemicals usage amount, also reduced drug cost, strengthened the market competitiveness of this prescription.
Embodiment: zineb and kasugarnycin composite
Further the present invention is made an explanation through following examples, but scope of the present invention is not limited to following examples.
1 materials and methods
1.1 test material
1.1.1 supply the reagent agent
The former medicine of 90% zineb, Hebei Shuangji chemical limited company produces; The former medicine of 55% kasugarnycin, Jilin Province prolongs limit spring thunder Bioceuticals Inc. and produces;
1.1.2 supply the examination pathogen
The cladosporium leaf and fruit mould of tomato bacterium, green house is gathered the cladosporium leaf and fruit mould of tomato leaf from Shouguang City, adopts tissue isolation to the pathogen separation and purification, in 4 ℃ of refrigerators, is cultivating preservation on the PSA slant medium.
1.1.3 supply the examination medium
PSA medium: potato 200g, sucrose 20g, agar 20g, water 1000ml
1.2 test method and synergistic effect are measured
1.2.1 two kinds of single agent suppress the toxicity test of tomato leaf fungal hyphae growth
The former medicinal an amount of pyridine dissolving of zineb is made into 1000 μ g/ml mother liquors, and is for use.Kasugarnycin dissolves with sterile water, and is for use.
On superclean bench, mother liquor is diluted to 5～6 concentration gradients; Accurately pipetting the 1mL soup joins in the P D A medium that melts about 9mL50 ℃; After mixing, pour into rapidly in advance in the diameter 9cm culture dish of sterilization, the cooling back the culture medium flat plate of pastille.
Cut the bacterium cake of the silk that carries disease germs with the diameter 5mm card punch of sterilization at the cladosporium leaf and fruit mould of tomato bacterium colony edge of cultivating 10 days, with transfer needle it is inserted on pastille culture medium flat plate, each culture dish is placed 1 bacterium cake.Each handles repetition 3 times.In 25 ℃ of insulating boxs, cultivate 11d, adopt the right-angled intersection method to measure colony diameter, get its mean value, calculate growth inhibition ratio.
Bacteriostasis rate (%)=((the clean amount of growth of the contrast bacterium colony-clean amount of growth of chemicals treatment bacterium colony)/clean amount of growth of contrast bacterium colony) * 100
Clean amount of growth=the colony diameter of bacterium colony-bacterium cake diameter
Concentration logarithm value for the reagent agent is an independent variable, is dependent variable with the relative inhibition probit value, carries out the data analysis statistics with the DPS data handling system, obtains virulence regression equation, EC
50Value and correlation coefficient.
On the basis of preliminary experiment, respectively single agent zineb and kasugarnycin are carried out toxicity test in order to last method, the two EC
50Value is respectively 15.6 μ g/ml and 2.8 μ g/ml.
Combination is provided with 1.2.2 be mixed
It is 48.65%+1.35%, 48%+2%, 46.2%+3.8%, 42.8%+7.2% and 37.5%+12.5% totally 5 combinations that are mixed that 50% zineb kasugarnycin wetting powder mass ratio is respectively 36: 1,24: 1,12: 1,6: 1 and 3: 1.
1.2.3 mixture toxicity test
Carry out the toxicity test of mixture according to mixed ratio with single agent toxicity test method.
EC that each that will measure according to the abundant method of Sun Yun handled
50Value is converted into the actual toxicity index (ATI) of using with; According to the proportioning of mixture, obtain theory and use toxicity index (TTI) with, calculate the co-toxicity coefficient (CTC) of mixture by following formula.
Single agent toxicity index=(standard medicament EC
50/ confession reagent agent EC
50) * 100
ATI=(standard medicament EC
50/ confession reagent agent (using with) EC
50) * 100
The content (%) of toxicity index * B in using with of content (the %)+B of toxicity index * A of TII=A in using with
CTC＝(ATI/TTI)×100
If co-toxicity coefficient is big by 120, showing has synergistic effect; If be starkly lower than 100 (below 80), be indicated as antagonism; Between 100～120, be indicated as summation action
Data are seen table 1
The different proportioning zineb of table 1 kasugarnycin mixture is to virulence and the synergistic effect of cladosporium leaf and fruit mould of tomato bacterium
The composite co-toxicity of table 2 zineb kasugarnycin to the cladosporium leaf and fruit mould of tomato bacterium
2 results and analysis
It is 136.9 that zineb and kasugarnycin are mixed at 24: 1 o'clock SR, shows as synergistic effect, and obviously greater than 120, all the other show as summation action.Comprehensive each side factor thinks that it is best proportioning that zineb and kasugarnycin were mixed 24: 1.
3 field trials are measured the control efficiency to cladosporium leaf and fruit mould of tomato and early blight of tomato
3.1 field trial is measured the control efficiency to cladosporium leaf and fruit mould of tomato
3.1.1 application method
Test is total to dispenser 3 times, check plot spray equivalent clear water.When making up a prescription, after with low amounts of water medicament fully being dissolved earlier, add suitable quantity of water again and carry out complete stool spraying processing, even spraying.All adopting power driven sprayer, operating pressure during the spray medicine is 2.0～2.3kg/cm
2, shower nozzle aperture 1.2mm.The sub-district spouting liquid is used by 675 liters/hectare, and soup is sprayed application to the positive and negative two sides of crop leaf uniformly.With the moistening blade of soup, not following is degree.
3.1.2 investigation method
Every sub-district is got 5 investigation at random, looks into 3 strains at every, and 5 on leaf is got in every strain upper, middle and lower, writes down total number of sheets, the sick number of sheets at different levels, calculates control efficiency.
Stage division is following:
0 grade: no scab;
1 grade, lesion area accounts for below 5% of whole leaf area;
3 grades, lesion area accounts for 10% of whole leaf area;
5 grades, lesion area accounts for 11%～20% of whole leaf area;
7 grades, lesion area accounts for 21%～50% of whole leaf area;
9 grades, lesion area accounts for more than 50% of whole leaf area.
3.1.3 control time and number of times
Investigate i.e. disease index investigation before the medicine, preventive effect investigation in 7 days behind the 1st medicine, 7 days preventive effect investigation behind the 2nd medicine altogether 3 times.
3.1.4 drug effect computational methods
Sick leaf rate (the %)=sick number of sheets/investigate total number of sheets * 100
Disease index=∑ (the sick numbers of sheets at different levels * relative level numerical value)/(investigating total number of sheets * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
3.1.5 direct influence to crop.
Whether have poisoning produce, will write down poisoning degree and type if any poisoning if observing crop.In addition, also should write down other useful influences to crop.
3.1.6 result of the test and analysis
Each handles the effect of preventing and treating cladosporium leaf and fruit mould of tomato table 3
Interpretation of result:
Can find out that by table 3 effect that 24: 1 the combination that is mixed of 50% zineb kasugarnycin wetting powder prevents and treats cladosporium leaf and fruit mould of tomato is more remarkable.Under the consumption of same every mu 20 gram, the effect of complex preparation significantly is superior to two kinds of single agent, and is the most outstanding with lasting effect.Complex preparation significantly is superior to the effect under the low concentration consumption in control efficiency under the consumption of high concentration.7 days control efficiency reaches 74.14% and 80.94% respectively behind the 1st, 2 medicine.And single agent zineb and kasugarnycin 7 days the highest preventive effect behind the 2nd medicine is respectively 69.24% and 75.86%, significantly inferior to complex preparation.Result of the test proves that complex preparation has significant synergistic effect under rational proportion, and the development of effectively having controlled the main disease of tomato is caused harm, and in production practices, has crucial meaning.According to the field range estimation, in the test dose scope, tomato growth is normal, and each is handled medicament and the poisoning phenomenon all do not occur.
3.2 field trial is measured the control efficiency to early blight of tomato
3.2.1 application method
Test is total to dispenser three times, check plot spray equivalent clear water.When making up a prescription, after with low amounts of water medicament fully being dissolved earlier, add suitable quantity of water again and carry out complete stool spraying processing, even spraying.All adopting power driven sprayer, operating pressure during the spray medicine is 2.0～2.3kg/cm
2, shower nozzle aperture 1.2mm.The sub-district spouting liquid is used by 675 liters/hectare, and soup is sprayed application to the positive and negative two sides of crop leaf uniformly.With the moistening blade of soup, not following is degree.
3.2.2 investigation method
Take five point samplings, the investigation of fixing a point, every sub-district random searching 5 points, 2 strains of every some investigation tomato, every strain branch upper, middle and lower are investigated 10 blades respectively for 3 layers, write down the total number of sheets and the sick number of sheets at different levels; Investigate the whole fruits of this 10 strain tomato simultaneously, record total fruit number and sick fruit number at different levels.
Sick leaf (or sick fruit) grade scale:
0 grade: no scab;
1 grade: lesion area accounts for whole blade (really) area below 5.0%;
3 grades: lesion area accounts for whole blade (really) area 5.1%～10.0%;
5 grades: lesion area accounts for whole blade (really) area 10.1%-20.0%;
7 grades: lesion area accounts for whole blade (really) area 20.1%-50.0%;
9 grades: lesion area accounts for whole blade (really) area more than 50.1%.
3.2.3 control time and number of times
Test is investigated 4 times altogether, i.e. disease index investigation before the medicine, preventive effect investigation in 10 days behind the 1st medicine, 14 days preventive effect investigation behind 10 days and the 3rd (last) medicine behind the 2nd medicine.
3.2.4 drug effect computational methods
Sick leaf rate (%)=sick leaf (really) count/is investigated total leaf (really) number * 100
Disease index=∑ (sick leaves at different levels (really) number * relative level numerical value)/(investigating total leaf (really) number * 9) * 100
Control efficiency (%)=(1-(disease index behind disease index before the blank district medicine * treatment region medicine)/(disease index before disease index behind the blank district medicine * treatment region medicine)) * 100
3.2.5 direct influence to crop.
Whether have poisoning produce, will write down poisoning degree and type if any poisoning if observing crop.
3.2.6 result of the test and analysis
Each handles the effect of preventing and treating early blight of tomato table 4
Interpretation of result:
Can find out that by table 4 effect of 24: 1 the black star of combination control pears that is mixed of 50% zineb kasugarnycin wetting powder is remarkable.Under the consumption of same every mu 20 gram, the effect of complex preparation significantly is superior to two kinds of single agent.Complex preparation significantly is superior to the effect under the low concentration consumption in control efficiency under the consumption of high concentration.14 days lasting effect is more remarkable after 10 days control efficiency behind the 1st, 2 medicine reaches 76.31% and 84.10%, the 3 medicine respectively, reaches 87.46%.And the highest preventive effect of single agent zineb and kasugarnycin is respectively 76.57% and 80.04%, significantly inferior to complex preparation.
In sum, the present invention contains the sterilization combination of zineb and kasugarnycin, cladosporium leaf and fruit mould of tomato and early blight of tomato are all had good control efficiency, and it is to the target crop safety.Containing cyproconazole in the complex preparation, is sterol demethylation inhibitor, has prevention and therapeutic action, to belong to the mould genus of spore, the Venturia bacterium is all effective.Zineb then is broad spectrum activity protection type bactericide, and is all effective to most of fungies.Complex preparation is had outstanding performance in test, has not only effectively suppressed further developing of germ, and through checking, has enlarged fungicidal spectrum, has delayed the drug-fast generation of germ.Comprehensive each item index shows that complex preparation significantly is superior to single agent, so research and development of the present invention and popularization have crucial meaning to society.
Claims (5)
1. bactericidal composition, it is characterized in that: contain two kinds of active components of zineb and kasugarnycin, wherein the weight ratio of zineb and kasugarnycin is 100: 1～1: 2.
2. bactericidal composition according to claim 1 is characterized in that: the more excellent weight ratio of zineb and kasugarnycin is 80: 1～1: 1.
3. bactericidal composition according to claim 1 is characterized in that: the percentage by weight that zineb and kasugarnycin are accumulated shared preparation is 5.5～75%.
4. bactericidal composition according to claim 1 is characterized in that: zineb and kasugarnycin and auxiliary agent and cosolvent are re-dubbed any one formulation that allows on the agricultural chemicals.
5. bactericidal composition according to claim 1 is characterized in that: the formulation that can be processed into is a wetting powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010258313 CN102369938B (en) | 2010-08-20 | 2010-08-20 | Sterilization composition containing zineb and chunleimeisu |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010258313 CN102369938B (en) | 2010-08-20 | 2010-08-20 | Sterilization composition containing zineb and chunleimeisu |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102369938A true CN102369938A (en) | 2012-03-14 |
| CN102369938B CN102369938B (en) | 2013-09-25 |
Family
ID=45789572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010258313 Active CN102369938B (en) | 2010-08-20 | 2010-08-20 | Sterilization composition containing zineb and chunleimeisu |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102369938B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104270948A (en) * | 2012-05-01 | 2015-01-07 | 帝斯曼知识产权资产管理有限公司 | Antifungal compositions |
| CN106614792A (en) * | 2016-12-27 | 2017-05-10 | 福建省农业科学院植物保护研究所 | Composition resistant to acidovorax citrulli |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3358001A (en) * | 1963-12-28 | 1967-12-12 | Umezawa Hamao | Antibiotic kasugamycin |
| CN1234179A (en) * | 1998-04-17 | 1999-11-10 | 罗姆和哈斯公司 | Improved dithiocarbamate bactericide composition |
-
2010
- 2010-08-20 CN CN 201010258313 patent/CN102369938B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3358001A (en) * | 1963-12-28 | 1967-12-12 | Umezawa Hamao | Antibiotic kasugamycin |
| CN1234179A (en) * | 1998-04-17 | 1999-11-10 | 罗姆和哈斯公司 | Improved dithiocarbamate bactericide composition |
Non-Patent Citations (1)
| Title |
|---|
| J.E.ADASKAVEG, ET AL.: "DETECTION OF ERWINIA AMYLOVORA STRAINS WITH REDUCED SENSITIVITY TO CURRENTLY REGISTERED ANTIBIOTICS AND MANAGEMENT OF FIRE BLIGHT OF POME FRUITS USING KASUGAMYCIN IN INTEGRATED STRATEGIES", 《ISHS ACTA HORTICULTURAE 793: XI INTERNATIONAL WORKSHOP ON FIRE BLIGHT》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104270948A (en) * | 2012-05-01 | 2015-01-07 | 帝斯曼知识产权资产管理有限公司 | Antifungal compositions |
| CN106614792A (en) * | 2016-12-27 | 2017-05-10 | 福建省农业科学院植物保护研究所 | Composition resistant to acidovorax citrulli |
| CN106614792B (en) * | 2016-12-27 | 2019-06-28 | 福建省农业科学院植物保护研究所 | A kind of composition of anti-acidovorax avenae subsp. citrulli |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102369938B (en) | 2013-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103053561B (en) | Benthiavalicarb isopropyl containing bactericidal composition | |
| CN101946802B (en) | Bactericide composition with synergistic effect | |
| CN101584329B (en) | 2-cyano-3-amino-3-phenylancryic acetate and propineb-containing synergistic antiseptic composition | |
| CN102919251B (en) | Bactericidal composition containing benthiavalicarb isopropyl and captan | |
| CN102369938B (en) | Sterilization composition containing zineb and chunleimeisu | |
| CN106857599A (en) | A kind of bactericidal composition containing pyraclostrobin and folpet and its application | |
| CN102334491A (en) | Sterilizing composition containing metiram and dimethomorph | |
| CN102835399A (en) | Metrafenone-containing bactericidal composition | |
| CN102715182A (en) | Sterilization composition containing active components Bellkute and imazalil | |
| CN102379291A (en) | Bactericidal combination containing difenoconazole and kasugamycin | |
| CN102293200A (en) | Sterilization composition containing zoxamide and copper pimaric acid | |
| CN102283225B (en) | Germicide composition containing coumoxystrobin and protective fungicide | |
| CN104823976A (en) | Pesticide composition containing bromothalonil and kasugamycin | |
| CN103947649B (en) | A kind of composition pesticide containing glyoxalin and kasugarnycin | |
| CN103190447B (en) | Sterilization composition | |
| CN102388875B (en) | Bactericidal composition containing coumoxystrobin and chlorothalonil | |
| CN103651453A (en) | Bactericidal composition containing hymexazol and carbendazim and application of bactericidal composition | |
| CN102334490A (en) | High efficiency sterilization composition containing mancozeb and flusilazole | |
| CN101822259A (en) | Sterilization compound containing ZJ0712 | |
| CN102258042A (en) | Germicide composition containing fludioxonil and thiophanate-methyl | |
| CN102334505A (en) | Bactericidal composition containing famoxadone and fosetyl aluminium | |
| CN106135243A (en) | A kind of fluorine-containing azoles bacterium aniline and the bactericidal composition of chlorine nalidixic bacterium ester | |
| CN102258040A (en) | Sterilization composition containing pyraclostrobin and polyoxin | |
| CN103300053B (en) | Bactericidal composition containing pyraclostrobin | |
| CN104604904A (en) | Bactericidal composition containing difenodiconazole and mancozeb |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |