CN115299440A - Bactericidal composition for preventing and treating bacterial angular leaf spot of muskmelon - Google Patents

Abstract

The invention relates to a bactericidal composition for preventing and treating bacterial angular leaf spot of melons, in particular to a bactericidal composition containing bromothalonil and bronopol, which is mainly characterized in that: the weight ratio of bromothalonil to bronopol is 3 to 1 to 3, the composition is prepared into wettable powder, and the dosage of effective active ingredients is 225-300 g/hectare.

Description

Bactericidal composition for preventing and treating bacterial angular leaf spot of muskmelon

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating bacterial angular leaf spot of melons.

Background

Bromothalonil is a novel compound with low toxicity, broad spectrum, sterilization, mildew prevention and algae removal performances, can inhibit and eradicate the growth of bacteria, fungi and algae, is suitable for the corrosion prevention, mildew prevention and industrial wastewater treatment of textiles, leather and the like, is also applied to the production of medicines and cosmetics abroad, is mainly applied to the prevention and control of crop diseases and the algae removal in fishponds and the industrial wastewater treatment at home, and is a specific agent for preventing and controlling various crop anthracnose in the current domestic bactericide.

The bronopol can effectively prevent and treat various plant pathogenic bacteria. The treatment of cotton seeds can prevent and control the black arm disease and bacterial wilt of cotton caused by cotton angular leaf spot pathogen, and has no chemical damage to cotton. Can also be used for treating rice bakanae disease.

The inventor finds that bromothalonil and bronopol with different action mechanisms are prepared into the wettable powder composition according to a certain proportion, so that the control effect on the bacterial angular leaf spot of the muskmelon can be effectively improved, the control cost is reduced, and the safety to crops is high.

Disclosure of Invention

The invention aims to provide a bactericidal composition for preventing and treating bacterial angular leaf spot of melons.

The technical scheme of the invention is as follows:

the bactericidal composition for preventing and treating the bacterial angular leaf spot of the muskmelon is characterized by comprising the following components in parts by weight: the effective active ingredients of the sterilization composition are bromothalonil and bronitol, wherein the weight ratio of the bromothalonil to the bronitol is 3:1-1:3, the preferred weight ratio is 1:1-1:3, and the optimal weight ratio is 1:1.

Further, the total weight of bromothalonil and bronopol in the bactericidal composition is 20-70%, the preferred total weight is 30-60%, and the best total weight is 50%.

Furthermore, the bactericidal composition contains bromothalonil, bronopol, a synergist and an auxiliary agent, and is prepared into wettable powder.

Further, the synergist is a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol or alkoxy modified polytrisiloxane, and the weight ratio of the synergist to the white carbon black is 1:1-3:1.

Further, the auxiliary agent is selected from one or more of a dispersing agent, a wetting agent, a defoaming agent, a pH regulator and a filler.

Furthermore, the dosage of the active ingredients of the sterilization composition is 225-300 g/hectare.

The dispersing agent is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, methylene dinaphthalene sodium sulfonate, lignin sodium salt or calcium salt, melamine formaldehyde resin, alkyl naphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphoric acid, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, dodecyl polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate, nonylphenol polyoxyethylene ether phosphate, naphthalenesulfonic acid formaldehyde condensate sodium salt block copolymer, comb-type polycarboxylate and sodium polycarboxylate;

the wetting agent is selected from one or more of sodium dodecyl benzene sulfonate, diisobutyl naphthalene sulfonate, sorbitan fatty acid ester, octyl sulfosuccinate sodium salt, alkyl succinic acid sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, sodium dodecyl sulfate, styryl phenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside and fatty alcohol polyoxyethylene ether sodium sulfonate;

the pH regulator is selected from one or more of glacial acetic acid, citric acid and potassium dihydrogen phosphate;

the defoaming agent is selected from one or more of organic silicone, fatty alcohol and fatty acid;

the filler is selected from: white carbon black, calcined kaolin, ammonium sulfate, potassium sulfate, magnesium sulfate, talcum powder and one or more of diatomite.

When the bactericidal composition is prepared into wettable powder, the bactericidal composition comprises the following components in percentage by weight: 5-52.5 percent of bromothalonil, 5-52.5 percent of bronopol, 1-20 percent of dispersing agent, 1-10 percent of wetting agent, 0-3 percent of defoaming agent, 0-4 percent of PH regulator and 100 percent of filler.

Compared with the prior art, the bactericidal composition for preventing and treating the bacterial angular leaf spot of the melon has the following beneficial effects: (1) Compared with a single agent, the composition has more remarkable effect on preventing and treating the bacterial angular leaf spot of the muskmelon; (2) The two compounds with different action mechanisms are mixed, so that the generation of the drug resistance of diseases can be effectively delayed, and the peach tree pesticide is good in safety and safe for peach trees.

Detailed Description

The invention will be further illustrated with reference to the following examples, in which the percentages are by weight, but the invention is not limited thereto.

Application example 1

EXAMPLE 1 50% bromothalonil-bronopol wettable powder

5363 g of 95% bromothalonil technical material 263.16 g, 3242 g of 95% bromonitrol technical material 263.16 g, 40g of alkyl naphthalene sulfonate formaldehyde polymer, 40g of lauryl sodium sulfate, 2 g of silicone defoaming agent, 60g of polyethoxy modified silane and polyethoxy modified fatty alcohol mixture, 100g of white carbon black and 1000 g of sodium sulfate are added to prepare the 50% bromothalonil-bromonitrol wettable powder.

EXAMPLE 2 50% bromothalonil-bronopol wettable powder

5363 g of 95% bromothalonil technical material 263.16 g, 3242 g of 95% bronopol technical material 263.16 g, 40g of alkoxy modified polytrisiloxane, 30g of white carbon black, 50 g of methyl naphthalene sulfonate formaldehyde condensate, 25 g of polycarboxylate, 20 g of fatty alcohol polyoxyethylene ether, 2 g of anhydrous citric acid and 1000 g of calcined kaolin are added to prepare the 50% bromothalonil-bronopol wettable powder.

EXAMPLE 3 50% bromothalonil-bronopol wettable powder

5363 g of 95% bromothalonil technical material 263.16 g, 3242 g of 95% bronopol technical material 263.16 g, 20 g of lignosulfonate, 40g of alpha-alkenyl sodium sulfonate, 80 g of alkoxy modified polytrisiloxane, 60g of white carbon black, 5 g of fatty acid, 80 g of ammonium sulfate and 1000 g of talcum powder, so as to prepare the 50% bromothalonil-bronopol wettable powder.

EXAMPLE 4 20% bromothalonil-bronopol wettable powder

5363 g of 95% bromothalonil original drug 52.63 g, 3242 g of 95% bronopol original drug 157.89 g, 50 g of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 20 g of white carbon black, 30g of alkyl naphthalene sulfonate, 30g of fatty alcohol polyoxyethylene ether, 100g of ammonium chloride and 1000 g of potassium sulfate are added to prepare the 20% bromothalonil-bronopol wettable powder.

EXAMPLE 5 30% bromothalonil-bronopol wettable powder

5363 g of 95% bromothalonil technical material 105.26 g, 3242 g of 95% bronopol technical material 210.53 g, 100g of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 50 g of white carbon black, 30g of polycarboxylate, 40g of alpha-alkenyl sodium sulfonate and 1000 g of diatomite are added to prepare the 30% bromothalonil and bronopol wettable powder.

EXAMPLE 6 wettable powder of 60% bromothalonil-bronopol

5363 g of 95% bromothalonil technical material 473.68 g, 3242 g of 95% bronopol technical material 157.89 g, 80 g of alkoxy modified polytrisiloxane, 60g of white carbon black, 40g of naphthalenesulfonic acid formaldehyde condensate sodium salt block copolymer, 30g of alkyl glycoside, 40g of fatty alcohol polyoxyethylene ether sodium sulfonate, 4 g of fatty acid and 1000 g of calcined kaolin are added to prepare the 60% bromothalonil-bronopol wettable powder.

EXAMPLE 7 70% bromothalonil-bronopol wettable powder

5363 g of 95% bromothalonil technical material 368.42 g, 3242 g of 95% bromonitroalcohol technical material 368.42 g, 50 g of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 40g of white carbon black, 30g of EO-PO block polyether, 30g of styryl phenol formaldehyde resin epoxypropane block polyether, 4 g of fatty acid and 1000 g of potassium sulfate are added to prepare 70% bromothalonil-bromonitroalcohol wettable powder.

The second application example is implemented:

toxicity determination of bromothalonil and bronopol mixture on melon bacterial angular leaf spot

According to the standards of NY/T1156.16-2008 and NY/T1156.6-2006, the toxicity of the bromothalonil and the bromonitrol and the proportion of 3:1, 2: 1, 1: 2 and 1:3 to the bacterial angular leaf spot pathogen of the melon are determined by a turbidity method, and the result shows that the bromothalonil and the bromonitrol are mixed according to the proportion of 5 to have a synergistic effect on the bacterial angular leaf spot pathogen of the melon. The experimental procedure was as follows:

purpose of the test

The toxicity of the bromothalonil and the bronopol and the mixed combination thereof in different proportions on the muskmelon bacterial angular leaf spot pathogen is measured indoors, the synergy evaluation is carried out, the suitability of the bromothalonil and the bronopol is determined, and scientific basis is provided for the research and development of the mixture of the bromothalonil and the bronopol.

2 conditions of the test

2.1 test targets

The melon bacterial angular leaf spot germ is preserved and provided by the research center of pesticide bioassay of the chemical research institute of Hunan province.

2.2 culture conditions

The culture conditions of the test target and the tested target are that the temperature is 25 +/-5 ℃ and the relative humidity is more than 65 +/-5%.

2.3 instrumentation

A beaker, a pipette, a triangular flask, an autoclave, a constant-temperature shaking incubator, an ultraviolet spectrophotometer and the like.

Design of the experiment

3.1 test Agents

Bromothalonil (bromothalonil) 95% bulk drug; bronopol (bronopol) 95% of the original drug.

3.2 drug concentration design and solution formulation

Bromothalonil solution: weighing 0.0100g 98% original drug of bromothalonil, dissolving with 0.5mL DMF, adding 189.5mL clear water containing 0.1% Tween80 emulsifier, stirring well to obtain 500mg/L mother liquor, and diluting with sterile water to concentrations of 250, 125, 62.5, 31.25, and 15.625mg/L for use;

bronopol solution: weighing 0.0100g of 95% original drug of bronopol, dissolving with 0.5mL of DMF, adding 189.5mL of water containing 0.1% of Tween80 emulsifier, stirring well to obtain 500mg/L mother liquor, and diluting with sterile water to concentrations of 250, 125, 62.5, 31.25 and 15.625mg/L for use;

each mixed solution: respectively adding 45mL, 30 mL, 25 mL, 15mL and 15mL of 500mg/L bromonitrol solution into 15mL, 25 mL, 30 mL and 45mL of 500mg/L bromothalonil solution to prepare 500mg/L bromonitrol-bromothalonil 3:1, 2: 1, 1: 2 and 1:3 solutions, and diluting with sterile water containing 0.1 percent Tween80 emulsifier to concentrations of 250 mg/L, 125 mg/L, 62.5 mg/L, 31.25 mg/L and 15.625mg/L for later use;

control solution: 0.5mL of DMF plus 99.5mL of sterile water containing 0.1% of Tween80 emulsifier.

4 test method

According to the standard method NY/T1156.16-2008 for biological detection, a turbidity method is adopted, wherein 5mL of liquid medicine with each single dose of series concentration is added into 45mL of NB culture medium cooled to 45 ℃ to prepare the medicine-containing culture medium with the required final concentration. Diluting the strain grown on the slant of NA culture medium to 1 × 10 with sterilized water ⁷ Each treatment medium was inoculated with 100ul of each spore/mL suspension, and each treatment was repeated 4 times. After the treatment, the mixture is placed at the temperature of 28-30 ℃ for shaking culture (120 r/min), and after 12 hours, the light absorption value is measured, and the growth inhibition rate is calculated.

5 data investigation and statistical analysis

5.1 methods of investigation

And (3) respectively measuring the light absorption value of each treatment before the culture is started, and measuring and recording the light absorption value of each treatment when the contrast treatment reaches the logarithmic phase. The wavelength measured was 660nm. The growth inhibition (%) was calculated.

In the formula: p represents growth inhibition rate; a. The ₀ Indicating an increase in absorbance value for the blank control; a. The ₁ Indicating the increase in absorbance of the agent treatment.

5.2 method for evaluating synergistic Effect

By referring to the indoor bioassay test criteria NY/T1156.6-2006 for pesticides, the synergy of the medicament mixture is evaluated according to the co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely CTC is not more than 80 as antagonistic action, 80-straw CTC-straw-type 120 is additive action, and CTC is not less than 120 as synergistic action.

Theoretical virulence index (TTI) = virulence index of agent A. Times% virulence index of agent A (%) + virulence index of agent B. RTM.

5.3 statistical analysis of data

All experimental data were analyzed using SAS6.12 statistical software. Calculating the hyphal growth inhibition rate (%) according to the test data, and solving a virulence regression equation, a correlation coefficient (r) and EC ₅₀ (95% confidence limit).

6 results analysis and discussion

6.1 evaluation of drug efficacy

The toxicity test results of the mixed combination of the bronopol and the bromothalonil in different proportions on the melon bacterial angular leaf spot are shown in table 1. As can be seen from Table 1, bromothalonil and bronopol in the ratio of 3:1, 2: 1, 1: 2 and 1:3 all showed synergistic effect on melon bacterial angular leaf spot, with EC thereof ₅₀ The values are 8.54, 7.94, 6.35, 6.03 and 5.79mg/L, respectively, and the co-toxicity coefficients are 135.78, 137.47, 153.85, 146.63 and 145.78, respectively. Wherein the two are mixed according to the proportion of 1:1 to achieve better synergistic effect.

TABLE 1 virulence determination results of bromothalonil and bronopol blending on melon bacterial angular leaf spot

6.2 discussion and conclusions

Indoor bioassay results show that the synergy effect is shown when the bronopol and the bromothalonil are mixed according to the proportion of 3:1, 2: 1, 1: 2 and 1:3, wherein the synergy effect is most obvious when the bronopol and the bromothalonil are mixed according to the proportion of 1:1, and therefore the proportion of 1:1 is the optimal proportion for mixing the bronopol and the bromothalonil.

Application example three: example 1 field efficacy experiment for preventing and treating melon bacterial angular leaf spot

The test conforms to the pesticide field efficacy test criterion (II), and the bactericide is used for preventing and treating the bacterial angular leaf spot of the melon (GB/T17980.110-2004).

Test crop of melon

Controlling bacterial angular leaf spot of target

1 basic information of the experiment

1.1 test name

The field efficacy test of 50% bromothalonil-bronitol wettable powder on melon bacterial angular leaf spot.

1.2 purpose of the test

The prevention and treatment effect, the applicable dosage and the safety of the 50% bromothalonil-bronopol wettable powder provided by the applicant on the melon bacterial angular leaf spot are determined, and a basis is provided for the registration and the popularization of test medicaments.

2 environmental and facility cultivation conditions

2.1 test site

Jiangxi province, nanchang City.

2.2 assay target conditions

Melon bacterial angular leaf spot (Pseudomonas syringae)

2.3 test crops, varieties and growth conditions

The muskmelon is a Changxiangyu jade, and has good growth vigor.

3 test design and arrangement

3.1 test amounts and Numbers

TABLE 2 test design of test agents

3.2 methods of application

3.2.1 application periods and methods

The use period is as follows: the first application is carried out 1 time before the onset of the bacterial angular leaf spot at the initial flowering stage of the muskmelon, the application is carried out 1 time at intervals of 7 days, and the application is continuously carried out 3 times.

3.2.2 Capacity of use

Spraying, wherein the dosage of the liquid medicine is 675 liters per hectare (45L per mu).

3.3 investigation methods, times and frequency

3.3.1 survey time and number of surveys

Investigation time: because the disease does not occur before the application (6 months and 1 day) and the disease condition is not clear when the application is applied for the 2 nd time, the control effect is investigated for 1 time only 10d (namely 6 months and 25 days) after the last application; the investigation times are as follows: 1 time.

3.3.2 methods of investigation

The diagonal of each cell is sampled at 5 points, each point is 3 plants, and the incidence of all leaves is investigated and classified according to the area percentage of lesion spots per leaf. . The blade grading method comprises the following steps:

no disease spot at level 0;

level 1: the lesion area accounts for less than 5% of the whole leaf area;

grade 3, the lesion spot area accounts for 6 to 10 percent of the whole leaf area;

grade 5, the lesion area accounts for 11 to 20 percent of the whole leaf area;

7 grade, the lesion area accounts for 21 to 50 percent of the whole leaf area;

and 9, stage: the lesion area accounts for more than 50% of the whole leaf area.

3.3.3 method for calculating drug Effect

Note CK ₀ 、CK ₁ Disease indexes before and after drug application in the blank control area are respectively; PT ₀ 、PT ₁ The disease indexes before and after the drug treatment area is applied are respectively.

4 results and analysis

TABLE 3 test results of the 50% bromothalonil-bronopol wettable powder for preventing and treating melon bacterial angular leaf spot

Test results show that the test medicament 50% bromothalonil-bronopol wettable powder has better control effect on the muskmelon bacterial angular leaf spot, and the control effect on the muskmelon bacterial angular leaf spot by 30, 35 and 40 g/mu treatment is 85.50%, 88.17% and 90.34% respectively. Statistical analysis shows that the control effect of the test medicament 40 g/mu is remarkably superior to that of the 30 g/mu treatment and the control medicament 25% bromothalonil wettable powder (dosage) 60 g/mu treatment and 20% bromonitrol wettable powder (dosage) 70 g/mu treatment, and the treatment is not remarkably different from that of the test medicament 30 g/mu treatment.

Application example four: EXAMPLE 2 efficacy test for controlling bacterial angular leaf spot of melon

The concrete experimental basis, experimental medicament, dosage and calculation method refer to the third application example.

1 test site

Guangxi farm courtyard.

2 test crops, varieties and growth conditions

The melon is Guimi No. 12. The growth was good.

3 method of application

3.1 application periods and methods

The test was performed 3 times. The application time is 8 months and 25 days, 9 months and 2 days and 9 months and 10 days in 2018, and the leaf period of the melon is 6 to the fruiting period.

3.2 Capacity of use

The amount of the liquid medicine sprayed per hectare is about 900 liters.

3.3 investigation time

The test is carried out for 3 times, the disease index before the medicine is investigated in 2018, 8 and 25 months, and the control effect is investigated in 2018, 9 and 10 months and 9 and 20 months.

3.4 methods of investigation

Randomly taking 15 plants per cell, investigating all leaves, and recording the total leaf number, the diseased leaf number and the disease grade of the investigation. Meter

Calculating the disease index and the prevention and treatment effect, and observing and recording the drug damage condition of the pesticide to the melons. Grading by percentage of lesion spots of each leaf in the whole leaf area, wherein the leaf grading method comprises the following steps:

no disease spot at level 0;

level 1: the lesion area accounts for less than 5% of the whole leaf area;

grade 3, the lesion spot area accounts for 6 to 10 percent of the whole leaf area;

grade 5, the lesion area accounts for 11 to 20 percent of the whole leaf area;

7 grade, the lesion spot area accounts for 21 to 50 percent of the whole leaf area;

and 9, stage: the lesion area accounts for more than 51% of the whole leaf area.

4 results and analysis

TABLE 4 wettable powder of 50% bromothalonil and bronopol for preventing and treating melon bacterial angular leaf spot test result 8 days after the 2 nd application

TABLE 5 wettable powder of bromothalonil and bronopol 50% for preventing and treating melon bacterial angular leaf spot test result 10 days after last application

In the investigation 8 days (9 months and 10 days) after the 2 nd application, the control effects of the test agent 50% bromothalonil and bronopol wettable powder on the bacterial angular leaf spot of the muskmelon are respectively 64.5%, 68.8% and 74.5%, and the control agent 25% bromothalonil wettable powder is 60 g/mu and the control agent 20% bromothalonil wettable powder is 70 g/mu are respectively 64.9% and 67.4%. The control effects of the test medicament 30 g/mu and 35 g/mu treatment areas are equivalent to those of the two control medicament treatment areas, the difference is not significant, the control effect of the test medicament 40 g/mu treatment area is superior to that of the control medicament 25% bromothalonil wettable powder 60 g/mu treatment area, the difference reaches 5% significant level, the control effect of the test medicament 20% bromonitroalcohol wettable powder 70 g/mu treatment area is equivalent, and the difference is not significant on 0.01 level.

In a survey 10 days (5 months and 20 days) after the 3 rd application, the control effects of the test medicament 50% bromothalonil and bronopol wettable powder on the bacterial angular leaf spot of the muskmelon are respectively 70.1%, 74.5% and 80.1% in 30, 35 and 40 g/mu treatment, and the control effects of the 25% bromothalonil wettable powder in 60 g/mu and the 20% bromothalonil wettable powder in 70 g/mu are respectively 68.1% and 72.2%. The control effects of the test medicament 30 g/mu and 35 g/mu treatment areas are equivalent to those of the two control medicament treatment areas, the difference is not significant, the control effect of the test medicament 40 g/mu treatment area is superior to that of the control medicament 25% bromothalonil wettable powder 60 g/mu and 20% bromonitrol wettable powder 70 g/mu treatment area, and the difference respectively reaches 1% extremely significant level and 5% significant level.

Application example five: EXAMPLES tests on melon safety

1.1 purpose of the test

Under indoor conditions, the safety of the 50% bromothalonil-bronopol wettable powder to the melon is evaluated, and scientific basis is provided for registration and use of the wettable powder on the melon.

1.2 test basis

Evaluation criteria for safety of agricultural chemicals to crops part 1: indoor test method for crop safety evaluation of bactericide and insecticide (NY/T1965.1-2010)

1.3 test site

Xian city Shanxi Meibang pesticide Co Ltd in laboratory for bioassay

1.4 test time

Test start time: 2016, 05 and 13 months

End time of the test: 2016 month 07 and 22 days

2 conditions of the test

2.1 selection of crops and cultivars

And (3) test crops: melon (sweet melon)

Variety: 208 melon, jinfei melon, elizabeth melon (commercially available)

3 test design and arrangement

3.1 design of the experiment

3.2 application time and frequency

The test is that the pesticide is sprayed once respectively in the vegetative growth period and the fruit expansion period of the muskmelon. The first pesticide application time of the test is 2016, 5 and 13 days, and the pesticide is applied for 1 time; the second time of the test is 7 months and 1 day, and the pesticide is sprayed once.

3.3 survey time and methods

Observing whether the pesticide is harmful to crops 7, 14 and 21 days after the last application, and mainly observing whether leaves have symptoms such as discoloration, necrosis, wilting, deformity and the like; the plant height, transverse diameter and longitudinal diameter of the melon are recorded 21 days before the drug administration and after the last drug administration.

3.4 growth Rate inhibition survey

Growth rate:

in the formula:

r: growth rate in millimeters per day (mm/d);

l: the height of the new plant is millimeter (mm);

d: is time in days (d).

Growth rate inhibition rate:

in the formula:

R _I : growth rate inhibition (%);

R _CK : blank control growth rate;

R _r : the agent treats the growth rate.

4 analysis of results

TABLE 6 influence of 50% bromothalonil-bronopol wettable powder on melon growth (208 in one-grade day)

TABLE 7 influence of 50% bromothalonil-bronopol wettable powder on melon growth (golden concubine)

TABLE 8 influence of 50% bromothalonil-bronitol wettable powder on melon growth (Elizabeth)

The experimental result shows that the safety of 50 percent bromothalonil-bronitol wettable powder to the muskmelon which is a test crop is determined in the vegetative growth period and the fruit expansion period by a spraying method, and the experimental result shows that: the 50% bromothalonil-bronopol wettable powder sprayed in the vegetative growth period is safe for the growth of plants of 208, jinfei and Elizabeth varieties in one day. The treatment of each medicament dose has no obvious inhibition effect on the growth of the tested melon plants, the melons grow normally under the recommended dose, twice or four times of the dose, leaves have no symptoms such as discoloration, necrosis, wilting, deformity and the like, and no phytotoxicity is generated during the test period.

The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, numerous simple deductions or substitutions may be made without departing from the spirit of the invention, which shall be deemed to belong to the scope of the invention.

Claims (9)

1. The bactericidal composition for preventing and treating the bacterial angular leaf spot of the muskmelon is characterized by comprising the following components in parts by weight: the effective active ingredients of the bactericidal composition are bromothalonil and bronitol, wherein the weight ratio of the bromothalonil to the bronitol is 3:1-1:3.

2. The germicidal composition of claim 1, wherein: the bactericidal composition contains bromothalonil, bronopol, a synergist and an auxiliary agent, and is prepared into wettable powder.

3. The germicidal composition as claimed in claim 1 or 2, wherein: the weight ratio of bromothalonil to bronopol is 1 to 1.

4. The germicidal composition of claim 3, wherein: the weight ratio of bromothalonil to bronopol is 1:1.

5. The germicidal composition as claimed in any one of claims 1-4, wherein: the total weight of bromothalonil and bronopol in the bactericidal composition is 20-70%.

6. The germicidal composition of claim 5, wherein: the total weight of bromothalonil and bronopol in the bactericidal composition is 30-60%.

7. The germicidal composition of claim 6, wherein: the total weight of bromothalonil and bronopol in the bactericidal composition is 50%.

8. The germicidal composition as claimed in claim 2, wherein: the auxiliary agent is selected from one or more of dispersing agent, wetting agent, binder, defoaming agent, pH regulator and filler.

9. The germicidal composition as claimed in any one of claims 1 to 8, wherein: the dosage of the effective components of the bactericidal composition is 225-300 g/ha.