CN115299454A - Bactericidal composition for preventing and treating melon downy mildew - Google Patents
Bactericidal composition for preventing and treating melon downy mildew Download PDFInfo
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- CN115299454A CN115299454A CN202210951445.2A CN202210951445A CN115299454A CN 115299454 A CN115299454 A CN 115299454A CN 202210951445 A CN202210951445 A CN 202210951445A CN 115299454 A CN115299454 A CN 115299454A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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Abstract
The invention relates to a bactericidal composition for preventing and treating melon downy mildew, in particular to a bactericidal composition containing benziothiazolinone and cymoxanil, which is mainly characterized in that: the weight ratio of the benziothiazolinone to the cymoxanil is 6 to 1, the composition is prepared into water dispersible granules, and the dosage of effective active ingredients is 52.5 to 157.5 g/hectare.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating melon downy mildew.
Background
Thiamphenone is a novel broad-spectrum bactericide, and is mainly used for preventing and treating various bacterial and fungal diseases such as cucumber downy mildew, pear scab, apple scab, citrus anthracnose, grape anthracnose and the like. The sterilization mechanism mainly comprises two aspects of destroying the nuclear structure of pathogenic bacteria, leading the pathogenic bacteria to lose heart parts and die due to failure, interfering the metabolism of pathogenic bacteria cells, leading the pathogenic bacteria cells to be disordered physiologically and finally leading the pathogenic bacteria cells to die.
Cymoxanil, the english generic name cymoxanil, chemical name: 1- (2-cyano-2-methoxyimino) -3-ethylurea. Cymoxanil is a highly effective, low toxicity fungicide, effective against the order Peronospora fungi such as Pegypsophila, peronospora, monochoria. Cymoxanil is used in combination with other protective bactericides and is suitable for preventing and treating diseases of vegetables such as melons, grapes, tomatoes, litchis and the like, fruit trees, tobaccos and the like.
The inventor finds that the water dispersible granule composition prepared from benziothiazolinone and cymoxanil with different action mechanisms according to a certain proportion can effectively improve the control effect on the downy mildew of melons, reduce the control cost and has high safety on crops.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and controlling melon downy mildew.
The technical scheme of the invention is as follows:
the effective active ingredients of the bactericidal composition are benziothiazolinone and cymoxanil, wherein the weight ratio of the benziothiazolinone to the cymoxanil is 6-1 to 6, and the preferable weight ratio is 1;
further, the bactericidal composition contains benziothiazolinone, cymoxanil, a synergist and white carbon black mixture and an auxiliary agent, and is prepared into water dispersible granules;
further, the total weight of the benziothiazolinone and the cymoxanil in the bactericidal composition is 35 to 77 percent, and the preferred total weight is 70 percent;
furthermore, the dosage of the effective components of the sterilization composition is 52.5 to 157.5 g/hectare.
Further, the synergist is a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol or alkoxy modified polytrisiloxane, and the weight ratio of the synergist to white carbon black is (1-3).
Furthermore, the auxiliary agent is selected from one or more of a dispersing agent, a wetting agent, a bonding agent, a defoaming agent, a pH regulator and a filler.
The dispersing agent is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, methylene dinaphthalene sodium sulfonate, lignin sodium salt or calcium salt, melamine formaldehyde resin, alkyl naphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphoric acid, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, dodecyl polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate, nonylphenol polyoxyethylene ether phosphate, naphthalenesulfonic acid formaldehyde condensate sodium salt block copolymer, comb-type polycarboxylate and sodium polycarboxylate;
the wetting agent is selected from one or more of sodium dodecyl benzene sulfonate, sodium diisobutylnaphthalene sulfonate, sorbitan fatty acid ester, sodium sulfosuccinate octyl ester, alkyl succinic acid sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, sodium dodecyl sulfate, styrylphenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside and fatty alcohol polyoxyethylene ether sodium sulfonate;
the binder is selected from one or more of water, starch, polyvinylpyrrolidone, polyethylene glycol, glucose and polyvinyl alcohol;
the pH regulator is selected from one or more of glacial acetic acid, citric acid, light calcium, potassium dihydrogen phosphate and triethanolamine;
the defoaming agent is selected from one or more of organic silicone, fatty alcohol and fatty acid;
the filler is selected from: one or more of white carbon black, calcined kaolin, ammonium sulfate, potassium sulfate, talcum powder, diatomite, starch and urea.
When the bactericidal composition is prepared into water dispersible granules, the bactericidal composition comprises the following components in percentage by weight: 5-66% of benziothiazolinone, 5-66% of cymoxanil, 3-20% of a mixture of a synergist and white carbon black, 1-20% of a dispersant, 1-10% of a wetting agent, 1-20% of a binder, 0-3% of a defoaming agent, 0-4% of a pH regulator and 100% of a filler.
Compared with the prior art, the bactericidal composition for preventing and treating melon downy mildew has the following beneficial effects: (1) Compared with a single agent, the composition has more remarkable effect on preventing and controlling the downy mildew of melons; (2) The two compounds with different action mechanisms are mixed, so that the generation of drug resistance of diseases can be effectively delayed, and the pesticide composition is good in safety and safe for melons.
Detailed Description
The invention will be further illustrated with reference to the following examples, in which the percentages are by weight, but the invention is not limited thereto.
Application example 1
Example 1 Thielanone cymoxanil Water dispersible granules 70%
10.20 percent of benziothiazolinone technical, 61.86 percent of cymoxanil technical, 3 percent of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 3 percent of white carbon black, 3 percent of EO-PO block polyether, 3 percent of alkyl naphthalene formaldehyde condensate sulfonate, 5 percent of sodium sulfosuccinate, 3 percent of starch, 0.3 percent of citric acid, 0.2 percent of silicone defoamer and 100 percent of calcined kaolin are added to prepare 70 percent of benziothiazolinone-cymoxanil water dispersible granule.
Example 2 Thielanone cymoxanil Water dispersible granules 70%
10.20 percent of benziothiazolinone original drug, 61.86 percent of cymoxanil original drug, 4 percent of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 2 percent of white carbon black, 4 percent of naphthalenesulfonic acid formaldehyde condensate sodium salt block copolymer, 2.5 percent of alkyl sulfonate, 3 percent of sodium dodecyl sulfate, 0.3 percent of polyvinylpyrrolidone, 0.2 percent of glacial acetic acid, 0.2 percent of fatty alcohol and starch which are added to 100 percent to prepare the 70 percent benziothiazolinone-cymoxanil water dispersible granule.
Example 3 Thielanone cymoxanil Water dispersible granules 70%
10.20 percent of benziothiazolinone original drug, 61.86 percent of cymoxanil original drug, 4 percent of alkoxy modified polytrisiloxane, 3 percent of white carbon black, 3 percent of comb-shaped polycarboxylate, 3 percent of alkyl polyoxyethylene ether sulfonate, 3 percent of phenethyl phenol polyoxyethylene ether phosphate, 2 percent of alkyl glucoside, 0.2 percent of organic silicone defoamer, 0.2 percent of polyethylene glycol and urea are added to 100 percent to prepare the 70 percent benziothiazolinone-cymoxanil water dispersible granule.
Example 4 Thielanone cymoxanil Water dispersible granules 70%
10.20 percent of benziothiazolinone original drug with the concentration of 98 percent, 61.86 percent of cymoxanil original drug with the concentration of 97 percent, 2 percent of alkoxy modified polytrisiloxane, 1.5 percent of white carbon black, 3.5 percent of polyoxyethylene polyoxypropylene ether block copolymer, 2 percent of sodium dodecyl benzene sulfonate, 2 percent of alkyl naphthalene sulfonate, 0.4 percent of fatty alcohol, 1 percent of polyvinyl alcohol, 0.2 percent of citric acid and 100 percent of potassium sulfate are added to prepare the 70 percent benziothiazolinone-cymoxanil water dispersible granule.
Example 5% Thielanone cymoxanil Water dispersible granules
11.22 percent of 98 percent of benziothiazolinone original drug, 68.04 percent of 97 percent of cymoxanil original drug, 2 percent of polyethoxy modified silane and polyethoxy modified fatty alcohol mixture, 2 percent of white carbon black, 4 percent of polyoxyethylene polyoxypropylene ether block copolymer, 3 percent of tristyrylphenol polyoxypropylene polyoxyethylene block polymer, 2 percent of fatty alcohol polyoxyethylene ether, 0.5 percent of glacial acetic acid, 0.2 percent of silicone defoamer and 100 percent of starch are added to prepare 77 percent of benziothiazolinone-cymoxanil water dispersible granules.
Example 6 Thielanone cymoxanil Water dispersible granules 50%
38.27 percent of 96 percent of benziothiazolinone original drug, 12.89 percent of 98 percent of cymoxanil original drug, 3 percent of alkoxy modified polytrisiloxane, 1.5 percent of white carbon black, 3 percent of fatty alcohol polyoxyethylene ether phosphate, 2 percent of methylene dinaphthalene sodium sulfonate, 5 percent of sorbitan fatty acid ester, 1 percent of polyethylene glycol, 0.1 percent of fatty acid, 5 percent of starch and 100 percent of diatomite to prepare the 50 percent of benziothiazolinone-cymoxanil water dispersible granule.
Example 7% Thielanone cymoxanil Water dispersible granules
5.10 percent of benziothiazolinone original drug, 30.93 percent of cymoxanil original drug, 5 percent of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 5 percent of white carbon black, 4 percent of styrene phenol polyoxyethylene ether phosphate, 3 percent of sodium polycarboxylate, 2.5 percent of alkylphenol formaldehyde resin polyoxyethylene ether, 2 percent of diisobutyl naphthalene sulfonate, 0.2 percent of silicone defoamer, 1.5 percent of glucose, 4 percent of ammonium sulfate and 100 percent of talcum powder, and the 35 percent of benziothiazolinone-cymoxanil water dispersible granule is prepared.
Example 8 Thielanone cymoxanil Water dispersible granules 35%
30.61 percent of 98 percent of benziothiazolinone original drug, 5.15 percent of 97 percent of cymoxanil original drug, 3 percent of alkoxy modified polytrisiloxane, 3 percent of white carbon black, 2 percent of alkylphenol polyoxyethylene ether phosphate, 4 percent of lignin sodium salt, 5 percent of castor oil polyoxyethylene ether, 0.7 percent of polyvinyl alcohol, 10 percent of ammonium sulfate, 0.3 percent of organic silicone defoamer and 100 percent of kaolin are added to prepare the 35 percent of benziothiazolinone-cymoxanil water dispersible granule.
The second application example is implemented:
toxicity determination of benziothiazolinone and cymoxanil mixed on muskmelon downy mildew
According to the standards of NY/T1156.3-2006 and NY/T1156.6-2006, the toxicity of the benziothiazolinone and the cymoxanil and the proportion of 1:1, 1: 3, 1:6, 6:1 and 3: 2 of the benziothiazolinone to the peronospora meloxicam is measured by a plate-leaf method, and the result shows that the benziothiazolinone and the cymoxanil are mixed according to the proportion of 5, so that the synergism on the meloxicam is shown. The experimental procedure was as follows:
purpose of the test
Toxicity of the benziothiazolinone and cymoxanil and the mixed combination thereof in different proportions on the melon peronospora parasitica are measured indoors, the synergistic effect evaluation is carried out, the suitability of the benziothiazolinone and the cymoxanil is determined, and scientific basis is provided for the research and development of the benziothiazolinone and cymoxanil mixed agent.
2 conditions of the test
2.1 test target
Peronospora melo (Pseudoperonospora cubensis (berk. Et curt.) Rostovzev), stored and supplied by Hainan Aimian detection, inc.
2.2 culture conditions
The culture conditions of the test target and the tested target are that the temperature is 17-22 ℃ and the relative humidity is more than 90%.
2.3 instrumentation
Beaker, flask, pipette, graduated cylinder, hemocytometer, microscope, crop sprayer, thousandth electron balance, and the like.
Design of the experiment
3.1 test Agents
Benziothiazolinone (98%) as a crude drug; cymoxanil (cymoxanil) 97% technical.
3.2 drug concentration design and solution formulation
Benziothiazolinone solution: weighing 0.0102g of 98% benziothiazolinone in a sterilized beaker, dissolving in 0.5mL of DMF, adding 99.5mL of sterile water containing 0.1% of Tween80 emulsifier, stirring well to prepare 100mg/L of mother liquor, and diluting with sterile water containing 0.1% of Tween80 emulsifier to a concentration of 10, 5, 1, 0.5, 0.1, 0.05mg/L for use;
cymoxanil solution: weighing 97% raw cymoxanil 0.0103g in a sterilized small beaker, dissolving with 0.5mL DMF, adding sterile water containing Tween80 0.1% of emulsifier 99.5mL, stirring well, preparing into 100mg/L mother liquor, and diluting with sterile water containing Tween80 0.1% of emulsifier to 10, 5, 1, 0.5, 0.1, 0.05mg/L concentration for later use;
each mixed solution: 10 mL, 5mL, 4 mL, 24 mL and 15mL of 10mg/L benziothiazolinone solution are respectively added into 10, 5, 4, 24 and 15mL of 10mg/L cymoxanil solution to prepare 10mg/L benziothiazolinone-cymoxanil solution with the concentration of 1:1, 1: 3, 1:6, 6:1 and 3:1, and then diluted into 5, 1, 0.5, 0.1 and 0.05mg/L concentration by using sterile water containing 0.1 percent of Tweenen 80 emulsifier for later use;
control solution: 0.5mL of DMF plus 99.5mL of sterile water containing 0.1% of Tween80 emulsifier.
4 test method
Referring to a raw measurement standard method NY/T1156.3-2006, a plate and blade method is adopted. Preparing a sporangia suspension: selecting diseased leaves, washing the downy mildew sporangium on the back of the leaves with distilled water at 4 ℃ to prepare suspension (the concentration is controlled to be 1 × 105 to 1 × 107 sporangiums per milliliter), and storing at 4 ℃ for later use.
Uniformly spraying the prepared liquid medicine on the back of the leaves, naturally drying the liquid medicine, then carrying out back-up treatment on the leaves of each treated leaf, and discharging the liquid medicine in a moisture preservation box according to treatment marks. At 24 hours after the treatment, 10. Mu.L of the prepared fresh sporangia suspension was dropped to the back of the leaf. 4 drops were inoculated per leaf, 5 leaves per treatment, and the test was blanked with treatments containing no agent. After inoculation, the inoculated cell is covered with a dish cover and placed in an artificial climate box, and the culture is carried out under the conditions that the continuous illumination/darkness is alternated for 12 hours every day, the temperature is 17-22 ℃, and the relative humidity is more than 90%.
5 data investigation and statistical analysis
5.1 methods of investigation
And (5) measuring and recording the diameter of the disease spots according to the disease condition of the blank control, and calculating the control effect (%).
In the formula: i represents the hypha growth inhibition rate; d 0 Indicates the growing diameter of the blank control colony; dt represents the agent-treated colony growth diameter.
5.2 evaluation method of synergistic Effect
By referring to indoor bioassay test criteria NY/T1156.6-2006 for pesticides, the synergism of the mixed pesticide is evaluated according to a co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely CTC is not more than 80 as antagonistic action, 80-straw CTC-straw-type 120 is additive action, and CTC is not less than 120 as synergistic action.
Theoretical virulence index (TTI) = virulence index of agent A. Times% virulence index of agent A (%) + virulence index of agent B. RTM.
5.3 statistical analysis of data
All test data were analyzed using DPS v9.50 statistical software. The hyphal growth inhibition (%) was calculated from the test data, and the virulence regression equation, the correlation coefficient (r) and the EC50 (95% confidence limit) were determined.
6 results analysis and discussion
6.1 evaluation of drug efficacy
The toxicity test results of the benziothiazolinone and cymoxanil mixed combination in different proportions on melon downy mildew are shown in Table 1. As can be seen from Table 1, the mixture of benziothiazolinone and cymoxanil in the ratio of 1:1, 1: 3, 1:6, 6:1 and 3:1 shows synergistic effect on the downy mildew of melon, the EC50 values are respectively 0.43, 0.33, 0.25, 0.56 and 0.47mg/L, the cotoxicity coefficients are respectively 129.76, 140.26, 174.61, 142.76 and 151.61, and the mixture of benziothiazolinone and cymoxanil in the ratio of 1:6 has better synergistic effect.
TABLE 1 toxicity test results of mixing of benziothiazolinone and cymoxanil for downy mildew of melon
6.2 discussion and conclusions
Indoor bioassay results show that the synergistic effect on the downy mildew germs of the muskmelon is realized by mixing the benziothiazolinone and the cymoxanil in the proportion of 1:1, 1: 3, 1:6, 6:1 and 3:1, wherein the synergistic effect of 1:6 is the best.
Application example three: example 1 field efficacy experiment for controlling melon downy mildew
The test refers to 'pesticide registration field efficacy test quality management standard' (NY/T2885-2016) and 'pesticide field efficacy test criterion (I)' part 26: the bactericide can be used for preventing and treating downy mildew of melon (GB/T17980.26-2000). The test process is strictly carried out according to the test scheme, and the report truly and accurately reflects the test result.
Test crop muskmelon
Downy mildew as a control target
1 basic information of the experiment
1.1 test name
The field efficacy test of 70% benziothiazolinone-cymoxanil water dispersible granules on the muskmelon downy mildew.
1.2 purpose of the test
The control effect, the applicable dosage and the safety of the 70% benziothiazolinone-cymoxanil water dispersible granule provided by the applicant on the downy mildew of melons are determined, and a basis is provided for registration and popularization of test medicaments.
2 environmental and facility cultivation conditions
2.1 test site
Tong in Chongqing City.
2.2 assay target conditions
Melon downy mildew (Pseudoperonospora cubensis (Berk. Et cut.) Rostov)
2.3 test crops, varieties and growth conditions
Muskmelon, and crisp pear muskmelon, wherein the muskmelon is in the tendril-like stage and the fruiting stage during the test. The melon has moderate planting density, regular arrangement, good water and fertilizer management, standard farm work operation, in-place cultivation management, timely weeding and good growth vigor.
3 test design and arrangement
3.1 test amounts and Numbers
TABLE 2 test design of test agents
3.2 methods of application
3.2.1 application periods and methods
Applying the medicine for 3 times, wherein the first application time is 2021, 05 and 16 days; the second application time is 2021, 05 and 26 days; the third application time is 2021 year, 06 months and 05 days.
3.2.2 Capacity of use
The amount of the liquid medicine sprayed per hectare is about 750 liters.
3.3 investigation methods, times and frequency
3.3.1 investigation time and number of surveys
And (4) phytotoxicity investigation: observing whether the muskmelon has phytotoxicity or not 3 days, 5 days and 7 days after each pesticide application.
The incidence of downy mildew of melons in each plot was investigated and recorded before each application (16 days at 05-month-2021, 26 days at 05-month-2021, 05 days at 06-month-2021) and 10 days after the third application (15 days at 06-month-2021). A total of 4 surveys were conducted.
3.3.2 methods of investigation
4 spots were randomly investigated for each cell, 2 plants were randomly investigated for each spot, and all leaves of each plant were investigated and recorded according to the following classification method.
No lesion spot is found at level 0;
stage 1: the lesion area accounts for less than 5% of the whole leaf area;
grade 3, the lesion area accounts for 6 to 10 percent of the whole leaf area;
grade 5, the lesion spot area accounts for 11 to 25 percent of the whole leaf area;
7 grade, the lesion area accounts for 26 to 50 percent of the whole leaf area;
and 9, stage: the lesion area accounts for more than 50% of the whole leaf area.
3.3.3 method for calculating drug effects
Note CK 0 -disease index of placebo zone; PT 0-disease index in treatment area.
4 results and analysis
TABLE 3 test results of 70% Thielanone-cymoxanil water dispersible granules for controlling downy mildew of melon 1
The investigation result before the third application of pesticide shows that under the test condition, the effective component dosage of the test reagent 70% benziothiazolinone-cymoxanil water dispersible granule is 52.5-157.5 g/ha, and after 3 times of spray treatment, the control effect on melon downy mildew is obvious, the disease leaf rate and disease index of plants treated by the reagent are obviously reduced compared with those of untreated plants, and the reagent has no adverse effect on other non-target organisms. Especially, the dosage of the active ingredients is more remarkable when 105-157.5 g/hectare (the dosage of the preparation is 10-15 g/mu).
Application example four: example 2 efficacy test for controlling downy mildew of melon
The third application example is referred to as specific experimental basis, experimental medicament and dosage, investigation method and calculation method.
1 test site
Shaanxi province, 37152es, yi district.
2 test crops, varieties and growth conditions
The melon is of the variety Zhongtian sweet No. 1. The growth was good.
3 method of application
3.1 application periods and methods
The test is carried out in 2021, the first application is carried out at 5-month and 16-day, the second application is carried out at 5-month and 23-day, and the third application is carried out at 5-month and 30-day; the application is carried out 3 times in total.
3.2 Capacity of use
The amount of the liquid medicine sprayed per hectare is about 750 liters.
3.3 investigation time
The test is carried out 2 times of investigation on disease condition base investigation before the first administration on 16 days at 5 months in 2021 and on prevention effect investigation 7 days after the last administration.
4 results and analysis
TABLE 4 test results of 70% Thielanone-cymoxanil water dispersible granules for controlling downy mildew of melon
The test medicament 70% benziothiazolinone-cymoxanil water dispersible granule in the embodiment 2 has an obvious control effect on the melon downy mildew, the control effect of 5-15 g/mu after the last application for 7 days reaches about 74-83%, the safety is good, the generation of the melon downy mildew can be effectively controlled, and the large-area demonstration and popularization of the water dispersible granule are suggested. The recommended preparation amount is 5 g/mu to 15 g/mu (the effective component amount is 52.5 g/hectare to 157.5 g/hectare).
Application example five: example 3 efficacy test for controlling downy mildew of melon
The third application example is referred to as specific experimental basis, experimental medicament and dosage, investigation method and calculation method.
1 test site
Yangling demonstration area, shaanxi province.
2 test crops, varieties and growth conditions
The melon has the variety of medium sweet No. 1 and has good growth condition.
3 method of application
3.1 application periods and methods
The test is carried out on the first application in 12 days in 7 months in 2019, the second application in 19 days in 7 months and the third application in 26 days in 7 months, and the application is carried out 3 times in total.
3.2 Capacity of use
The amount of the liquid medicine sprayed per hectare is about 900 liters.
3.3 investigation time
The test is carried out for 2 times of investigation on disease condition base investigation before the first administration on 12 days in 7 months in 2019 and on prevention effect investigation 7 days after the last administration.
4 results and analysis
TABLE 5 test results of 70% Thielanone-cymoxanil water dispersible granules for controlling downy mildew of melon
The test medicament 70% benziothiazolinone-cymoxanil water dispersible granule in the embodiment 3 has the control effect of about 74-83% after being treated for 7 days after the last application, has good safety, can effectively control the generation of melon downy mildew, and is recommended to be popularized in a large scale. The recommended dose is as follows: the effective component amount is 52.5 g/ha to 157.5 g/ha (preparation amount: 5 g/mu to 15 g/mu).
Application example six: example 4 efficacy test for controlling downy mildew of melon
The third application example is referred to as specific experimental basis, experimental medicament and dosage, investigation method and calculation method.
1 test site
Yongchuan area of Chongqing City.
2 test crops, varieties and growth conditions
The melon is white-sand honey melon.
3 method of application
3.1 application periods and methods
Applying the medicine for 3 times, wherein the first application time is 7 months and 12 days; the second application time is 7 months and 22 days; the third application time is 8 months and 1 day.
3.2 Capacity of use
Spraying stem and leaf with the amount of the pesticide liquid being about 750 liters per hectare.
3.3 investigation time
The occurrence of downy mildew of melons in each plot was investigated and recorded before the first application and 10 days after each application for a total of 4 times.
4 results and analysis
TABLE 6 test result of 70% benziothiazolinone-cymoxanil water dispersible granules for controlling melon downy mildew
As can be seen from Table 6, the test medicament 70% benziothiazolinone cymoxanil water dispersible granule in example 4 has an obvious control effect on melon downy mildew after 3 times of spraying treatment, the morbidity and disease index of the plants after the medicament treatment are obviously reduced compared with the plants without the treatment, and the medicament has no adverse effect on other non-target organisms.
Application example seven: examples 1-4 safety test report for melon
1 basic conditions of the test
1.1 purpose of the test
Under indoor conditions, the safety of the 70% benziothiazolinone-cymoxanil water dispersible granules to the melon is evaluated, and scientific basis is provided for registration and use of the benziothiazolinone-cymoxanil water dispersible granules on the melon.
1.2 test basis
Section 1 of evaluation criteria for safety of agricultural chemicals to crops: indoor test method for crop safety evaluation by bactericide and insecticide (NY/T1965.1-2010)
1.3 test site
Building No. A19 of grass beach three-way stone sheep industrial area of central area of xi 'an city, xi' of Shanxi province
1.4 test time
Test start time: 2018, 06 and 13 months
End time of the test: 09 and 22 months in 2018
2 conditions of the test
2.1 selection of crops and cultivars
And (3) test crops: melon (sweet melon)
Variety: 208 melon, jinfei melon, elizabeth melon (commercially available)
3 test design and arrangement
3.1 design of the experiment
3.2 application time and frequency
The test is that the pesticide is sprayed once respectively in the melon vegetative growth period and the melon fruit enlargement period. The first pesticide application time of the test is 2018, 6 and 13 days, and the pesticide is sprayed for 1 time; the second time of the test is 7 months and 25 days, and the pesticide is sprayed once.
3.3 survey time and methods
Observing whether the plant is harmed by the pesticide 7, 14 and 21 days after the last application, and mainly observing whether the leaves have symptoms such as discoloration, necrosis, wilting and deformity; the plant height, transverse diameter and longitudinal diameter of the melon were recorded 21 days before and after the last dose.
3.4 growth Rate inhibition survey
Growth rate:
in the formula:
r: growth rate in millimeters per day (mm/d);
l: the height of the new plant is millimeter (mm);
d: is time in days (d).
Growth rate inhibition rate:
in the formula:
R I : growth rate inhibition (%);
R CK : blank control growth rate;
R r : the agent treatment growth rate.
4 analysis of results
TABLE 7 influence of 70% Thielanone-cymoxanil Water dispersible granules on melon growth (208 on top of a day)
TABLE 8 influence of 70% Thiazone cymoxanil Water dispersible granules on melon growth (Jinfei)
TABLE 9 influence of 70% Thielanone-cymoxanil Water dispersible granules on melon growth (Elizabeth)
The experimental result shows that the safety of the 70% benziothiazolinone-cymoxanil water dispersible granule to the test crop melon is measured in the vegetative growth period and the fruit expansion period by a spraying method, and the experimental result shows that: the spraying of 70% benziothiazolinone-cymoxanil water dispersible granules in the vegetative growth period is safe for the growth of plants of 208, jinfei and Elizabeth varieties in one day. The treatment of each medicament dose has no obvious inhibition effect on the growth of test melon plants, the melon plants grow normally under the recommended dose, twice or four times of the dose, leaves have no symptoms such as discoloration, necrosis, wilting, deformity and the like, and no phytotoxicity is generated during the test period.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Claims (7)
1. The bactericidal composition for preventing and treating melon downy mildew is characterized by comprising the following components in parts by weight: the effective active ingredients of the bactericidal composition are benziothiazolinone and cymoxanil, wherein the weight ratio of the benziothiazolinone to the cymoxanil is 6-1.
2. The germicidal composition as claimed in claim 1, wherein: the bactericidal composition contains benziothiazolinone, cymoxanil, a synergist, a white carbon black mixture and an auxiliary agent, and is prepared into water dispersible granules.
3. The germicidal composition as claimed in claim 1 or 2, wherein: the weight ratio of the benziothiazolinone to the cymoxanil is 1.
4. The germicidal composition as claimed in any one of claims 1-3, wherein: the total weight of the benziothiazolinone and the cymoxanil in the bactericidal composition is 35-77%.
5. The germicidal composition of claim 4, wherein: the total weight of the benziothiazolinone and the cymoxanil in the bactericidal composition is 70%.
6. The germicidal composition of claim 2, wherein: the auxiliary agent is selected from one or more of dispersing agent, wetting agent, binder, defoaming agent, pH regulator and filler.
7. The germicidal composition as claimed in any one of claims 1 to 6, wherein: the dosage of the effective components of the bactericidal composition is 52.5 to 157.5 grams per hectare.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210951445.2A CN115299454A (en) | 2022-08-09 | 2022-08-09 | Bactericidal composition for preventing and treating melon downy mildew |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210951445.2A CN115299454A (en) | 2022-08-09 | 2022-08-09 | Bactericidal composition for preventing and treating melon downy mildew |
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