CN113207888A

CN113207888A - Sterilization composition containing methoxybenzene cry bacterium and metconazole and application thereof

Info

Publication number: CN113207888A
Application number: CN202110510845.5A
Authority: CN
Inventors: 金立; 张帮林; 贾增坡; 葛琳; 高瞻; 范成松
Original assignee: Anhui Fengle Agrochemical Co Ltd
Current assignee: Anhui Fengle Agrochemical Co Ltd
Priority date: 2021-05-11
Filing date: 2021-05-11
Publication date: 2021-08-06

Abstract

The invention discloses a bactericidal composition containing methoxybenzene cry strain and metconazole, which comprises the effective components of methoxybenzene cry strain and metconazole, wherein the mass ratio of the methoxybenzene cry strain to the metconazole is 1-20: 32-2. The invention also discloses application of the bactericidal composition containing the methoxybenzene cry bacterium and metconazole to prevention and treatment of diseases in crops. According to the invention, the methoxybenzene cry bacterium and the metconazole are compounded, so that the two can generate a synergistic effect, and the formed bactericidal composition has a good bactericidal effect, and particularly has a good control effect on tomato sclerotiniose. Compared with single dose of the metoxybenzene cry strain and the metconazole, the bactericidal composition has low dosage and cost, and simultaneously, the side effect of the bactericidal composition is low, and the safety, the yield and the like of crops cannot be influenced.

Description

Sterilization composition containing methoxybenzene cry bacterium and metconazole and application thereof

Technical Field

The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing methoxybenzene cry bacterium and metconazole and application thereof.

Background

Tomato sclerotiniose is a disease caused by Sclerotinia sclerotiorum (sclerotiotinia sclerotiorum) and occurring in tomato. Mainly harms the base of the stem, can also harm the stem, the leaves, the petioles, the flowers, the fruits and the fruit stalks, and can be infected with diseases in both the seedling stage and the adult stage. The blade infection mostly occurs from the leaf margin, the blade is in a light green water diffuse shape at first, then the blade is changed into grey brown, and white hypha appears; the fruits are burnt by boiled water and grow thick hypha, and the rat manure-shaped sclerotia appears on the fruits at the later stage; the stem infection mostly invades from the base of the petiole, the disease spot is brown, the edge of the disease spot is soaked in water, white hypha grows when the humidity is high, a large amount of sclerotia can be formed inside and outside the stem in the later period, and the plant withers when the humidity is high. The disease is common on tomatoes in a greenhouse, and if the disease is not prevented and controlled in time, flower and fruit fall can be caused, and the yield of the tomatoes is reduced by 20-30% seriously.

Methoxybenzene cry bacterium (flubendiamide) belongs to SHID bactericide, and can inhibit the formation of pathogenic bacteria attached spore and subsequent mycelium infiltration into plant cells; the application can inhibit the formation of pathogenic bacteria secondary hypha, mycelium and spore after the disease appears. Inhibitors of C-14 demethylase in the biosynthesis of metconazol (metconazol) ergosterol. The biosynthesis of the wheat pyrosterol is inhibited, the permeability and the membrane structure of a fungal cell membrane are damaged, and the formation of spores and the growth of hyphae are inhibited.

The methoxybenzene cry bacterium and metconazole both have high bactericidal activity, are mainly used for preventing and controlling cereal powdery mildew caused by powdery mildew of gramineae worldwide, and lack corresponding activity and prevention effect research on diseases caused by sclerotinia sclerotiorum. Therefore, in the prior art, different medicaments are combined to control diseases, but the effect of the combination of different medicaments is uncertain, and the combination of different medicaments can produce synergistic effect and antagonistic effect. And the effect of combinations of different agents on different diseases is not determined. No compound medicament of the combination of the methoxybenzene cry bacterium and the metconazole aiming at the tomato sclerotiniose exists in the prior art.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a sterilizing composition containing methoxybenzene cry bacterium and metconazole and application thereof.

The purpose of the invention is realized by the following technical scheme:

the sterilizing composition comprises the effective components of methoxybenzene cry strain and metconazole, and the mass ratio of the methoxybenzene cry strain to the metconazole is 1-20: 32-2. Further, the mass ratio of the methoxybenzene cry strain to the metconazole is 5: 8. By adopting the proportion, the bactericidal effect of the bactericidal composition can be further ensured, and the control effect of the bactericidal composition on tomato sclerotiniose and the like can be further improved.

According to a preferable technical scheme, the methoxybenzene cry bacterium accounts for 1-20% of the total mass of the bactericidal composition, and the metconazole accounts for 2-32% of the total mass of the bactericidal composition. More preferably, the methoxybenzene cry bacterium accounts for 10% of the total mass of the bactericidal composition, and the metconazole accounts for 16% of the total mass of the bactericidal composition.

Preferably, the dosage form of the bactericidal composition is a liquid preparation. Further, the liquid formulation is a suspending agent. The bactericidal composition further comprises the following substances in percentage by mass: 4 to 7 percent of dispersant, 2 to 6 percent of wetting agent, 0.1 to 0.5 percent of thickening agent, 1 to 3 percent of preservative, 1 to 6 percent of antifreeze agent and the balance of water. Further, the content of each substance is as follows by mass percent: 5% of dispersing agent, 5% of wetting agent, 0.2% of thickening agent, 1.5% of preservative, 4% of antifreeze agent and the balance of water.

The invention also aims to provide application of the bactericidal composition containing the methoxybenzene cry strain and metconazole, and the bactericidal composition is used for preventing and treating diseases in crops. Further preferably, the bactericidal composition is used for preventing and treating tomato sclerotinia rot.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, the methoxybenzene cry bacterium and the metconazole are compounded, so that the two can generate a synergistic effect, and the formed bactericidal composition has a good bactericidal effect, and particularly has a good control effect on tomato sclerotiniose. Compared with single dose of the metoxybenzene cry strain and the metconazole, the bactericidal composition has low dosage and cost, and simultaneously, the side effect of the bactericidal composition is low, and the safety, the yield and the like of crops cannot be influenced.

Detailed Description

The present invention will be further described with reference to the following examples. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The embodiment of the invention provides a sterilization composition, and the raw materials of the active ingredients of the sterilization composition consist of methoxybenzene cry bacteria and metconazole. In the embodiment of the invention, the methoxybenzene cry bacterium and the metconazole are specifically selected for compounding, and the two can generate a synergistic effect, so that the formed bactericidal composition has a good bactericidal effect, a large control spectrum and a good control effect on sclerotiniose in particular. Meanwhile, the bactericidal composition has low side effect and cannot influence the safety, the yield and the like of crops.

It should be noted that, although the embodiment of the present invention uses the bactericidal composition as a suspending agent, it does not mean that the bactericidal composition is only used as a suspending agent when used for controlling diseases, and it is understood that other dosage forms such as emulsifiable concentrate, dispersible oil suspending agent, water dispersible granule, microemulsion, etc. are also within the protection scope of the embodiment of the present invention.

The dispersant provided by the embodiment of the invention includes but is not limited to the existing dispersant such as sodium lignosulfonate, the wetting agent includes but is not limited to the existing surfactant such as sodium dodecyl sulfate, the thickening agent includes but is not limited to the existing thickening agent such as xanthan gum, the preservative includes but is not limited to the existing preservative such as sodium benzoate, and the antifreeze includes but is not limited to the existing antifreeze such as ethylene glycol.

The disease for preventing and controlling the bactericidal composition provided by the invention comprises but is not limited to tomato sclerotiniose and the like, but the bactericidal composition has better effect on preventing and controlling the tomato sclerotiniose. Similarly, the bactericidal composition is directed to crops including but not limited to tomatoes and also various field economic crops.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

The embodiment of the invention provides a bactericidal composition, which comprises the effective components of methoxybenzene cry strain and metconazole, and the mass ratio of methoxybenzene cry strain to metconazole is 1: 32.

Example 2

The embodiment of the invention provides a bactericidal composition, which comprises the effective components of methoxybenzene cry strain and metconazole, and the mass ratio of the methoxybenzene cry strain to the metconazole is 2: 16.

Example 3

The embodiment of the invention provides a bactericidal composition, which comprises the effective components of methoxybenzene cry strain and metconazole, and the mass ratio of the methoxybenzene cry strain to the metconazole is 5: 8.

Example 4

The embodiment of the invention provides a bactericidal composition, which comprises the effective components of methoxybenzene cry strain and metconazole, and the mass ratio of the methoxybenzene cry strain to the metconazole is 5: 2.

Example 5

The embodiment of the invention provides a bactericidal composition, which comprises the effective components of methoxybenzene cry strain and metconazole, and the mass ratio of the methoxybenzene cry strain to the metconazole is 10: 1.

Example 6

The embodiment provides a bactericidal composition, which comprises the following components in percentage by mass:

TABLE 1 fungicidal compositions

Name of ingredient	Content (%)
		Methoxybenzene cry bacteria	1
Metconazole	32
		Lignosulfonic acid sodium salt	5
Sodium dodecyl sulfate	4
		Xanthan gum	0.2
Sodium benzoate	1.5
		Ethylene glycol	5
Water (W)	Make up the balance

Example 7

TABLE 2 composition of the bactericidal composition

Name of ingredient	Content (%)
		Methoxybenzene cry bacteria	2
Metconazole	16
		Lignosulfonic acid sodium salt	5
Sodium dodecyl sulfate	4
		Xanthan gum	0.13
Sodium benzoate	1.5
		Ethylene glycol	4
Water (W)	Make up the balance

Example 8

TABLE 3 the ratio of the components in the bactericidal composition

Example 9

TABLE 4 composition of bactericidal composition

Name of ingredient	Content (%)
		Methoxybenzene cry bacteria	15
Metconazole	6
		Lignosulfonic acid sodium salt	6
Sodium dodecyl sulfate	3
		Xanthan gum	0.17
Sodium benzoate	1.5
		Ethylene glycol	5
Water (W)	Make up the balance

Example 10

TABLE 5 composition of the composition

Name of ingredient	Content (%)
		Methoxybenzene cry bacteria	20
Metconazole	2
		Lignosulfonic acid sodium salt	5
Sodium dodecyl sulfate	3.8
		Xanthan gum	0.2
Sodium benzoate	1.7
		Ethylene glycol	5
Water (W)	Make up the balance

Experimental example 1 biological Activity test

According to the indoor bioassay test criteria NY/T1156.1-2006, NY/T1156.2-2006 and NY/T1156.6-2006 of the pesticide, the optimal mixing mass ratio of the two bactericides is screened by measuring the effect of the mixture of the methoxybenzene cry bacterium, the metconazole and different mass ratios thereof on the sclerotiniose.

The tomato Sclerotinia sclerotiorum (sclerotiorum) is provided by the separation and preservation of the Ministry of agricultural technology of Ministry of Nuanhui Feng le; the test agents are methoxybenzene cry strain raw drug and metconazole raw drug; methoxybenzene cry bacteria: the mixing proportion of the metconazole is as follows: 1: 32; 1: 8; 5: 8; 5: 2; 10: 1; the preparation concentrations are shown in Table 6:

TABLE 6 dosage ratio of each substance of the bactericidal composition

Under aseptic operation conditions, quantitatively adding a pre-melted sterile culture medium into an aseptic conical flask according to test treatment, quantitatively sucking liquid medicines from low concentration to high concentration in sequence, respectively adding into the conical flasks, and sufficiently shaking. Then pouring the mixture into 4 culture dishes with the diameter of 9cm in equal quantity to prepare the drug-containing plates with corresponding concentrations.

The experiment was run with a treatment of 0.1% aqueous tween 80 containing an equal amount of acetone solution as a blank, repeated 4 times per treatment.

And (3) cutting the cultured pathogenic bacteria from the edges of bacterial colonies by using a sterilization puncher with the diameter of 5mm under the aseptic condition, inoculating the bacterial cake to the center of a drug-containing flat plate by using an inoculator, covering a dish cover, and culturing in an incubator at 25 ℃.

And (5) investigating the growth condition of the hyphae of the pathogenic bacteria according to the growth condition of the bacteria in the blank control culture dish. The colony diameter was measured in millimeters (mm) with a caliper. The diameter of each colony was measured perpendicularly by the cross method once and averaged.

According to the investigation result, calculating the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested according to the formulas (1) and (2), wherein the unit is percentage (%), and calculating the result to reserve two decimal points:

D＝D₁-D₂ (1)

in the formula:

d, colony growing diameter;

D₁-colony diameter;

D₂-cake diameter.

In the formula:

i-inhibition rate of hypha growth;

D₀-blank control colony growth diameter;

D_t-agent treatment colony growth diameter.

3.5.2 statistical analysis

Performing regression analysis according to the log of each agent concentration and the corresponding hypha growth inhibition rate value, and calculating EC of each agent₅₀、EC₉₀And 95% confidence limits.

And calculating the synergy coefficient (SR) of the mixture according to the Wadley method, and evaluating the combined action type of the mixture. The synergistic effect of the mixed medicaments is evaluated according to the synergistic coefficient (SR), namely, the SR < 0.5 is antagonistic action, the SR < 0.5 and less than or equal to 1.5 are additive action, and the SR > 1.5 is synergistic action. The synergistic coefficient (SR) is calculated according to the following equations (3) and (4):

in the formula:

X₁mixture EC₅₀Theoretical values in milligrams per liter (mg/L);

P_A-percentage of a in the admixture;

P_B-percentage of B in the admixture;

A-EC of A in the mixture₅₀Values in milligrams per liter (mg/L);

B-EC of B in the mixture₅₀Values are in milligrams per liter (mg/L).

In the formula:

SR-synergistic coefficient of the mixture;

X₁mixture EC₅₀Theoretical values in milligrams per liter (mg/L);

X₂mixture EC₅₀Measured in milligrams per liter (mg/L).

The results are shown in Table 7 below:

TABLE 7 test results

According to the results, the single dose of the methoxybenzene cry bacterium and the metconazole and the mixing proportion thereof have obvious bactericidal effect on the tomato sclerotiniose. The synergistic effect is shown in the experimental mixture ratio after the metoxybenzene cry strain and the metconazole are mixed, wherein the synergistic effect is highest when the ratio of the metoxybenzene cry strain to the metconazole is 5:8, the synergistic coefficient is 3.07, and LC (liquid chromatography) is adopted₅₀Is 0.244mg/L, LC₉₀It was 2.564 mg/L.

Experimental example 2 field drug effect test

In the field efficacy test I, the bactericidal composition prepared in the application example 8 is used for preventing and treating tomato sclerotinia rot in the field.

1. The test method comprises the following steps:

the test agents are shown in table 8 below:

TABLE 8 Scale for each drug test

The test site is arranged in Feitong county of Hefei city, Anhui province, and the test crop tomato is planted in the greenhouse. When the pesticide is applied, the tomato grows normally, the growth vigor is relatively vigorous, and the tomato grows in the middle stage of young fruits. The disease condition is that the tomato sclerotiniose is in an initial state when the drug is applied. The application conditions and method are that the application is started in 2019 at 4-6 months, and the application times are 2 times. Day 1 was 4 months and 6 days, and day 2 was 4 months and 13 days. The application method adopts the labor

Spraying with 16 model agricultural knapsack hand sprayer, each 667m²The amount of water used was 72 kg. The average maximum temperature during the period was 22 ℃ and the average minimum air temperature was 14 ℃. The soil type is ditch dry mud, the pH value is neutral, and the organic matter content is medium. The soil is moist, and no mulching film is covered on the surface of the tomato ridge. Investigation content and method (1) pharmacodynamic investigation, wherein the disease condition base is investigated at the beginning of the experiment, then the medicine is applied, the prevention effect is investigated for 1 time every 7 days, and the disease condition and the prevention and treatment results are investigated for 7 days after the last application. And (4) safety investigation, namely observing whether the pesticide has phytotoxicity on crops and whether dwarfing, chlorosis, deformity and the like occur.

2 results of the test

The control effect of different medicament treatments on tomato sclerotiniose is shown in table 9 below.

TABLE 9 control of tomato sclerotinia rot by different drug treatments

According to the table, the control effect investigation result shows that the control effect of the preparation with the dosage of 20 ml/mu is the best in the example treatment of 8, the control effect of 7 days after the second time of the application is 93.54%, and the control effects of the three agents in the example are obviously better than those of the two control agents. Through field observation, the test agent and the control agent are safe to test crops, namely tomatoes, and no phytotoxicity symptoms (such as dwarfing, chlorosis, deformity and the like) are found.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sterilization composition containing methoxybenzene cry bacteria and metconazole, which is characterized in that: the active ingredients of the bactericidal composition are methoxybenzene cry bacteria and metconazole, and the mass ratio of the methoxybenzene cry bacteria to the metconazole is 1-20: 32-2.

2. The bactericidal composition containing the methoxybenzene cry bacterium and metconazole according to claim 1, wherein: the mass ratio of the methoxybenzene cry strain to the metconazole is 5: 8.

3. The bactericidal composition containing the methoxybenzene cry bacterium and metconazole according to claim 1, wherein: the methoxybenzene cry bacterium accounts for 1-20% of the total mass of the bactericidal composition, and the metconazole accounts for 2-32% of the total mass of the bactericidal composition.

4. The bactericidal composition containing methoxybenzene cry bacteria and metconazole according to claim 3, wherein: the methoxybenzene cry bacterium accounts for 10% of the total mass of the bactericidal composition, and the metconazole accounts for 16% of the total mass of the bactericidal composition.

5. The fungicidal composition containing methoxybenzene cry bacterium and metconazole according to any one of claims 1 to 4, wherein: the dosage form of the bactericidal composition is a liquid preparation.

6. The bactericidal composition containing methoxybenzene cry bacteria and metconazole according to claim 5, wherein: the bactericidal composition further comprises the following substances in percentage by mass: 4 to 7 percent of dispersant, 2 to 6 percent of wetting agent, 0.1 to 0.5 percent of thickening agent, 1 to 3 percent of preservative, 1 to 6 percent of antifreeze agent and the balance of water.

7. The use of the fungicidal composition containing methoxybenzene cry bacterium and metconazole according to claim 6, wherein: the bactericidal composition is used for preventing and treating diseases in crops.

8. The use of the fungicidal composition containing methoxybenzene cry bacterium and metconazole according to claim 7, characterized in that: the bactericidal composition is used for preventing and treating tomato sclerotiniose.