CN115316393A - Application of bactericidal composition in preventing and treating bacterial angular leaf spot of melons - Google Patents
Application of bactericidal composition in preventing and treating bacterial angular leaf spot of melons Download PDFInfo
- Publication number
- CN115316393A CN115316393A CN202211086325.7A CN202211086325A CN115316393A CN 115316393 A CN115316393 A CN 115316393A CN 202211086325 A CN202211086325 A CN 202211086325A CN 115316393 A CN115316393 A CN 115316393A
- Authority
- CN
- China
- Prior art keywords
- bronopol
- kasugamycin
- leaf spot
- test
- bactericidal composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- LVDKZNITIUWNER-UHFFFAOYSA-N Bronopol Chemical compound OCC(Br)(CO)[N+]([O-])=O LVDKZNITIUWNER-UHFFFAOYSA-N 0.000 claims abstract description 50
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
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- 229920001568 phenolic resin Polymers 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
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Abstract
The invention relates to a bactericidal composition for preventing and treating bacterial angular leaf spot of melons, in particular to a bactericidal composition containing kasugamycin and bronopol, which is mainly characterized in that: the weight ratio of kasugamycin to bronopol is 9 to 1 to 9, the composition is prepared into water dispersible granules, and the dosage of effective active ingredients is 112.5 to 157.5 g/hectare.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to an application of a bactericidal composition for preventing and treating bacterial angular leaf spot of melons.
Background
Kasugamycin is an agricultural fungicide. Has excellent control effect and treatment effect on rice blast on rice. Has special effects of preventing and treating diseases such as bacterial angular leaf spot of watermelon, gummosis of peach trees, scab and perforation.
The bronopol can effectively prevent and treat various plant pathogenic bacteria. The treatment of cotton seeds can prevent and control the black arm disease and bacterial wilt of cotton caused by cotton angular leaf spot pathogen, and has no chemical damage to cotton. Can also be used for treating rice bakanae disease.
The melon bacterial angular leaf spot is caused by pseudomonas syringae cucumber angular leaf spot pathotype and occurs in melon. Mainly harms leaves, petioles, stems, tendrils and fruits, and can occur from the seedling stage to the adult stage. The cotyledon shows water-soaked spots at the early stage of disease, the spots become light brown, and the spots are polygonal under the restriction of the vein; small water-soaked spots appear when the stem, the petiole, the tendril and the like are attacked, and longitudinally expand along the stem ditch to form a short strip shape; when fruits develop diseases, small water-soaked spots appear, and after the fruits expand, the small spots are irregular or connected, and a large amount of white purulence is overflowed from the diseased part. The melon bacterial angular spot has different pathogeny environments and regions and larger difference of the degree of disease. The condition of the disease is primarily humidity, especially rain, as saturated humidity is large and typical of lesions over 6 hours.
The inventor finds that the kasugamycin and the bronopol with different action mechanisms are prepared into the water dispersible granule composition according to a certain proportion, so that the muskmelon bacterial angular leaf spot can be effectively prevented and treated, the prevention and treatment cost is reduced, and the safety to crops is high.
Disclosure of Invention
The technical difficulty solved by the invention is as follows: the bacterial angular leaf spot of the melon mainly harms leaves of crops and has strong infectivity, if the bacterial angular leaf spot is not effectively treated, the bacterial angular leaf spot can harm new and old leaves, the leaves are crisp, the photosynthesis is reduced, and the product and the quality are influenced. The prior art has very few medicaments for preventing or controlling the bacterial angular leaf spot of the muskmelon, mainly antibiotics and copper preparations, but the antibiotics have strong drug resistance due to frequent use, and the copper preparation is easy to burn leaves and cause phytotoxicity in the use process.
The invention aims to provide application of a bactericidal composition for preventing and treating bacterial angular leaf spot of melons, wherein the bactericidal composition has both protection and treatment effects and is safe to crops.
The technical scheme of the invention is as follows:
the application of the bactericidal composition in preventing and treating the bacterial angular leaf spot of the melon is characterized in that: the effective active components of the bactericidal composition are kasugamycin and bronopol, wherein the weight ratio of the kasugamycin to the bronopol is 9-1.
Further, the total weight of the kasugamycin and the bronopol in the bactericidal composition is 10-60%, the preferred total weight is 20-40%, and the best total weight is 30%.
Further, the sterilization composition contains kasugamycin, bronopol, a synergist and an auxiliary agent, and is prepared into water dispersible granules.
Furthermore, the auxiliary agent is selected from one or more of a dispersing agent, a wetting agent, a bonding agent, a defoaming agent, a pH regulator and a filler.
Furthermore, the dosage of the effective components of the sterilization composition is 112.5 to 157.5 g/hectare.
The synergist is selected from one of polyethoxy modified silane, polyethoxy modified fatty alcohol mixture or alkoxy modified polytrisiloxane;
the dispersing agent is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, methylene dinaphthalene sodium sulfonate, lignin sodium salt or calcium salt, melamine formaldehyde resin, alkyl naphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphoric acid, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, dodecyl polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate, nonylphenol polyoxyethylene ether phosphate, naphthalenesulfonic acid formaldehyde condensate sodium salt block copolymer, comb-type polycarboxylate and sodium polycarboxylate;
the wetting agent is selected from one or more of sodium dodecyl benzene sulfonate, sodium diisobutylnaphthalene sulfonate, sorbitan fatty acid ester, sodium sulfosuccinate octyl ester, alkyl succinic acid sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, tristyrylphenol polyoxypropylene polyoxyethylene block polymer, sodium dodecyl sulfate, styrylphenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside and fatty alcohol polyoxyethylene ether sodium sulfonate;
the binder is selected from one or more of water, polyvinylpyrrolidone, polyethylene glycol, glucose, polyvinyl alcohol and starch;
the pH regulator is selected from one or more of light calcium, glacial acetic acid, citric acid and potassium dihydrogen phosphate;
the defoaming agent is selected from one or more of organic silicone, fatty alcohol and fatty acid;
the filler is selected from: white carbon black, calcined kaolin, ammonium sulfate, potassium sulfate, magnesium sulfate, sodium sulfate, talcum powder, diatomite, ammonium chloride, urea and starch.
When the bactericidal composition is prepared into water dispersible granules, the bactericidal composition comprises the following components in percentage by weight: 1-10% of kasugamycin, 1-54% of bronopol, 1-8% of synergist, 1-20% of dispersant, 1-10% of wetting agent, 1-20% of binder, 0-3% of defoamer, 0-4% of pH regulator and 100% of filler.
Compared with the prior art, the bactericidal composition for preventing and treating the bacterial angular leaf spot of the melon has the following beneficial effects: (1) Compared with a single agent, the composition has more remarkable synergistic effect on preventing and controlling the bacterial angular leaf spot of the melon; (2) The two compounds with different mechanisms of action are mixed, so that the protective and control effects are good; (3) The composition forms hypochlorous acid when entering water, has good sterilization effect, quick action and lasting effect, and the lasting period can reach more than 20 days; (4) The traditional Chinese medicine can be used for treating more than one kind of diseases, and can prevent and treat various fungal and bacterial diseases.
Detailed Description
The invention will be further illustrated with reference to the following examples, in which the percentages are by weight, but the invention is not limited thereto.
Application embodiment 1
Example 1A 30% Chunleimycin-bronopol Water dispersible granule
46.15 g of 65% kasugamycin technical material, 284.21 g of 95% bronopol technical material, 20 g of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 40 g of sodium salt or calcium salt of lignin, 25 g of comb-type polycarboxylate, 40 g of sodium dodecyl benzene sulfonate, 30 g of alkyl naphthalene sulfonate, 30 g of white carbon black, 10 g of citric acid, 6 g of organic silicone defoamer, 50 g of calcined kaolin, 50 g of urea and 1000g of diatomite, thus preparing the 30% kasugamycin-bronopol water dispersible granule.
Example 2 Chunleimeisu-bronopol Water dispersible granules 30%
46.15 g of 65% kasugamycin technical material, 284.21 g of 95% bronopol technical material, 30 g of alkoxy modified polytrisiloxane, 30 g of EO-PO block polyether, 20 g of sodium polycarboxylate, 40 g of sorbitan fatty acid ester, 30 g of alkyl sulfate, 30 g of polyethylene glycol, 15 g of monopotassium phosphate, 10 g of fatty alcohol, 50 g of potassium sulfate and 1000g of ammonium chloride are added to prepare the 30% kasugamycin-bronopol water dispersible granule.
Example 3A 30% Chunleimycin-bronopol Water dispersible granule
46.15 g of 65% kasugamycin technical, 284.21 g of 95% bronopol technical, 15 g of mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 30 g of sodium methylene dinaphthalene sulfonate, 45 g of alkyl naphthalene sulfonate, 50 g of sodium sulfosuccinate, 20 g of alkyl glycoside, 30 g of polyethylene glycol, 10 g of fatty alcohol, 150 g of ammonium sulfate and 1000g of starch, thus preparing the 30% kasugamycin-bronopol water dispersible granule.
Example 4% Chunleimycin Brornitrol Water dispersible granules
76.92 g of 98% kasugamycin technical material, 157.89 g of 95% bronopol technical material, 30 g of alkoxy modified polytrisiloxane, 30 g of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, 35 g of dodecyl polyoxyethylene ether phosphate, 20 g of sodium dodecyl benzene sulfonate, 25 g of styryl phenol formaldehyde resin propylene oxide block polyether, 15 g of polyvinyl alcohol, 20 g of glacial acetic acid, 50 g of starch, 12 g of fatty acid, 80 g of ammonium sulfate, 50 g of urea and 1000g of calcined kaolin are added to prepare the 20% kasugamycin-bronopol water dispersible granule.
Example 5% Chunleimycin Brornitrol Water dispersible granule
61.54 g of 98% kasugamycin technical material, 378.95 g of 95% bronopol technical material, 40 g of alkylphenol polyoxyethylene ether phosphate, 25 g of naphthalenesulfonic acid formaldehyde condensate sodium salt block copolymer, 20 g of triphenylethylphenol polyoxypropylene polyoxyethylene block polymer, 30 g of sodium dodecyl sulfate, 50 g of polyvinylpyrrolidone, 25 g of citric acid, 50 g of monopotassium phosphate, 10 g of fatty alcohol, 90 g of starch, 50 g of talcum powder and adding diatomite to 1000 to prepare the 40% kasugamycin-bronopol water dispersible granule.
Example 6 Water dispersible granules of kasugamycin Brornitrool 60%
92.31 g of 98% kasugamycin technical, 568.42 g of 95% bronopol technical, 10 g of alkoxy modified polytrisiloxane, 25 g of alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, 40 g of comb-type polycarboxylate, 15 g of fatty alcohol polyoxyethylene ether, 70 g of alkyl glucoside, 50 g of glucose, 10 g of organic silicone and 1000g of starch, thus obtaining the 60% kasugamycin-bronopol water dispersible granule.
The second application example is implemented:
toxicity determination of kasugamycin and bronopol mixing on bacterial angular leaf spot of melon
According to the standards of NY/T1156.16-2008 and NY/T1156.6-2006, the toxicity of the kasugamycin and the bronopol to the bacterial angular leaf spot pathogenic bacteria of the melon is measured by a turbidity method and the proportion of the kasugamycin to the bronopol is 1:1, 1:3, 3:1, 1:9 and 9:1, and the result shows that the kasugamycin and the bronopol are mixed according to the above 5 proportions, and the synergy is shown to the bacterial angular leaf spot pathogenic bacteria of the melon. The experimental procedure was as follows:
purpose of the test
The toxicity of the kasugamycin and the bronopol and the mixed combination thereof in different proportions on the bacterial angular leaf spot pathogen of the melon is measured indoors, the synergistic effect evaluation is carried out, the suitability of the kasugamycin and the bronopol is determined, and the scientific basis is provided for the research and the development of the mixed agent of the kasugamycin and the bronopol.
2 test conditions
2.1 test target
The melon bacterial angular leaf spot germ is preserved and provided by the research center of pesticide bioassay of the chemical research institute of Hunan province.
2.2 culture conditions
The culture conditions of the test target and the tested target are that the temperature is 25 +/-5 ℃ and the relative humidity is more than 65 +/-5%.
2.3 instrumentation
A beaker, a pipette, a triangular flask, an autoclave, a constant-temperature shaking incubator, an ultraviolet spectrophotometer and the like.
Design of the experiment
3.1 test Agents
Kasugamycin (kasugamycin) 65% technical; bronopol (bronopol) 95% technical.
3.2 drug concentration design and solution formulation
Kasugamycin solution: weighing 0.1000g of kasugamycin 65% original drug, dissolving with 0.5mL of DMF, adding 129.5mL of clear water containing 0.1% of Tween80 emulsifier, stirring uniformly to prepare 500mg/L mother liquor, and then releasing with sterile water to concentrations of 250, 125, 62.5, 31.25 and 15.625mg/L for later use;
bronopol solution: weighing 0.1500g95% bronopol raw drug, dissolving with 1mL of DMF, adding 284mL of clear water containing 0.1% Tween80 emulsifier, stirring uniformly to prepare 500mg/L mother solution, and diluting with sterile water containing 0.1% Tween80 emulsifier to concentrations of 250, 125, 62.5, 31.25 and 15.625mg/L for later use;
each mixed solution: respectively adding 25 mL, 36 mL, 12 mL, 45mL and 5mL of 500mg/L kasugamycin solution into 25 mL, 12 mL, 36 mL, 5mL and 45mL of 500mg/L bronopol solution to prepare 500mg/L bronopol-kasugamycin 1:1, 1:3, 3:1, 1:9 and 9:1 solutions, and diluting with sterile water containing 0.1 percent tween80 emulsifier to concentrations of 250 mg/L, 125 mg/L, 62.5 mg/L, 31.25 mg/L and 15.625mg/L for later use;
control solution: 0.5mL of DMF plus 99.5mL of sterile water containing 0.1% of Tween80 emulsifier.
4 test method
According to the standard method of biological measurement NY/T1156.16-2008, a turbidity method is adopted, wherein 5mL of liquid medicine with each single dose of concentration is added into 45mL of NB culture medium cooled to 45 ℃ to prepare the medicine-containing culture medium with the required final concentration. Diluting the strain grown on the slant of NA culture medium to 1 × 10 with sterilized water 7 Each treatment medium was inoculated with 100ul of each spore/mL suspension, and each treatment was repeated 4 times. After the treatment, the mixture is placed at the temperature of 28-30 ℃ for shaking culture (120 r/min), and after 12 hours, the light absorption value is measured, and the growth inhibition rate is calculated.
5 data investigation and statistical analysis
5.1 methods of investigation
And (3) respectively measuring the light absorption value of each treatment before the culture is started, and measuring and recording the light absorption value of each treatment when the contrast treatment reaches the logarithmic phase. The wavelength measured was 660nm. The growth inhibition (%) was calculated.
In the formula: p represents a growth inhibition rate; a. The 0 Indicating an increase in absorbance value for the blank control; a. The 1 Indicating the increase in absorbance of the agent treatment.
5.2 evaluation method of synergistic Effect
By referring to indoor bioassay test criteria NY/T1156.6-2006 for pesticides, the synergism of the mixed pesticide is evaluated according to a co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely CTC is not more than 80 as antagonistic action, 80-straw CTC-straw-type 120 is additive action, and CTC is not less than 120 as synergistic action.
Theoretical virulence index of mixture (TTI) = virulence index of agent a × percent (%) of agent a + virulence index of agent B XB% in the mixture
5.3 statistical analysis of data
All experimental data were analyzed using SAS6.12 statistical software. Calculating the hyphal growth inhibition rate (%) according to the test data, and solving a virulence regression equation, a correlation coefficient (r) and EC 50 (95% confidence limit).
6 results analysis and discussion
6.1 evaluation of drug efficacy
The toxicity test results of the kasugamycin and the bronopol which are mixed and combined in different proportions on the bacterial angular leaf spot of the melon are shown in table 1. As can be seen from Table 1, kasugamycin and bronopol were mixed in a ratio of 1:1, 1:3, 3:1, 1:9 and 9:1 with respect to muskmelon finesThe mycorrhizal angular leaf spot bacteria all show synergistic effect, and the EC thereof 50 The values are 7.07, 5.52, 8.16, 4.30 and 4.49mg/L respectively, and the co-toxicity coefficients are 126.80, 134.18, 139.25, 155.96 and 132.13 respectively. Wherein the two are mixed according to the proportion of 1:9 to achieve better synergistic effect.
TABLE 1 determination of virulence of the mixture of kasugamycin and bronopol against bacterial angular leaf spot of melon
6.2 discussion and conclusions
Indoor bioassay results show that the kasugamycin and the bronopol are mixed according to the following ratio of 1, 3, 1, 9 and 9, and show synergistic effect on the peach bacterial angular leaf spot germs, wherein the mixing synergistic effect of the two is most obvious according to the ratio of 1.
Application example three: example 1 field efficacy experiment for preventing and treating melon bacterial angular leaf spot
The test conforms to the quality management standard of pesticide registration test and the experimental criteria of GB/T17980.110-2004 pesticide field efficacy (II) that the bactericide prevents and treats cucumber bacterial angular leaf spot.
Test crop of melon
Controlling bacterial angular leaf spot of target
1 basic information of the experiment
1.1 test name
The 30% kasugamycin and bronopol water dispersible granule is used for field drug effect test of melon bacterial angular leaf spot.
1.2 purpose of the test
The prevention and treatment effect, the applicable dosage and the safety of the 30% kasugamycin-bronopol water dispersible granule provided by the applicant on the bacterial angular leaf spot of the melon are determined, and a basis is provided for the registration and the popularization of a test medicament.
2 environmental and facility cultivation conditions
2.1 test site
Yangling demonstration area, shaanxi province.
2.2 assay target conditions
Melon bacterial angular leaf spot (Pseudomonas syringae pv.)
2.3 test crops, varieties and growth conditions
The melon is sweet and treasure, and has good growth vigor.
3 test design and arrangement
3.1 test dosage and numbering
TABLE 2 test design of test agents
3.2 methods of application
3.2.1 application periods and methods
The stem and leaf of the plant are evenly sprayed at the early stage of disease occurrence. The application is carried out for 3 times, the first application time is 05 and 11 days in 2019, 18 days in 5 months, the second application time is 25 days in 05 months.
3.2.2 Capacity of use
Spraying, the spraying liquid amount per hectare is about 800 liters.
3.3 investigation methods, times and frequency
3.3.1 survey time and number of surveys
The test is carried out for 2 times of investigation on disease condition base investigation before first application in 5-11 days in 2019 and prevention effect investigation 7 days after last application.
3.3.2 methods of investigation
2 trees are surveyed in each cell, 2 new shoots are selected from each region in 5 directions in east, west, south and north of each tree, and the total leaf number and the number of diseased leaves are recorded. The blade grading method comprises the following steps:
no lesion spot is found at level 0;
stage 1: the lesion area accounts for less than 5% of the whole leaf area;
grade 3, the lesion spot area accounts for 6 to 10 percent of the whole leaf area;
grade 5, the lesion spot area accounts for 11 to 20 percent of the whole leaf area;
7 grade, the lesion spot area accounts for 21 to 50 percent of the whole leaf area;
and 9, stage: the lesion area accounts for more than 51% of the whole leaf area.
3.3.3 method for calculating drug Effect
Note CK 0 、CK 1 Disease indexes before and after drug application in the blank control area are respectively; PT 0 、PT 1 The disease indexes before and after the drug treatment area is applied are respectively.
4 results and analysis
TABLE 3 test results of 30% kasugamycin and bronopol water dispersible granule for preventing and treating melon bacterial angular leaf spot
The test result shows that the test medicament 30% kasugamycin-bronopol water dispersible granule has better control effect on the bacterial angular leaf spot of the melon, and the average control effect of 35 g/mu, 30 g/mu and 25 g/mu after being treated for 7 days after the last medicament is 84.97%, 79.81% and 75.12% respectively. 70 g/mu of 20% bronopol wettable powder of the control medicament and 55 g/mu of 6% kasugamycin wettable powder of the control medicament have the average control effects of 78.97% and 73.83% respectively after the treatment of 7 days after the last medicament.
The control effect 7 days after the last drug is subjected to biological statistical analysis by adopting a Duncan's New negative range (DMRT) method, and the variance analysis result shows that the difference among treatments reaches an extremely significant level and the difference among repetitions is not significant. The difference comparison results show that the difference significance comparison results are consistent at the 0.05 level and the 0.01 level. The difference between 35 g/mu of test medicament 30% kasugamycin-bronopol water dispersible granule and other treatments reaches an extremely significant level; the difference between 30 g/mu of treatment of the test medicament 30% kasugamycin and bronopol water dispersible granule and 70 g/mu of treatment of the control medicament 20% bronopol wettable powder is not significant, but the difference between the two treatments and other treatments reaches a very significant level; the differences between 25 g/mu of test medicament 30% kasugamycin-bronopol water dispersible granules and 55 g/mu of control medicament 6% kasugamycin wettable powder are not obvious, but the differences between the test medicament and the control medicament and other treatments reach an extremely obvious level.
In conclusion, the test medicament 30% kasugamycin-bronopol water dispersible granules have good prevention and treatment effects on the bacterial angular leaf spot of the melon, the average prevention and treatment effect of 25-35 g/mu 7 days after the last medicament reaches about 75-85%, the safety is good, and the occurrence of the bacterial angular leaf spot of the melon can be effectively controlled.
Application example four: example 2 efficacy test for controlling bacterial angular leaf spot of melon
The third application example is referred to for concrete experimental basis, experimental medicament and dosage, investigation method and calculation method.
1 test site
Yangling demonstration area, shaanxi province.
2 test crops, varieties and growth conditions
The melon is of the variety Zhongtian sweet No. 1. The growth was good.
3 method of application
3.1 application periods and methods
The test is carried out in 2018, the first application is carried out on day 14 in 6 months, the second application is carried out on day 21 in 6 months, and the third application is carried out on day 28 in 6 months; the application was performed 3 times in total.
3.2 Capacity of use
The spraying liquid amount per hectare is about 800 liters.
3.3 investigation time
The test is carried out for 2 times of investigation on the disease condition base before the first administration on 14 days in 6 months in 2018 and on the control effect investigation 7 days after the last administration.
4 results and analysis
TABLE 4 test results of 30% kasugamycin and bronopol water dispersible granules for preventing and treating melon bacterial angular leaf spot
Test results show that the test medicament 30% kasugamycin-bronopol water dispersible granule has better control effect on melon bacterial angular leaf spot, and the average control effects of 35 g/mu, 30 g/mu and 25 g/mu after treatment for 7 days after the last medicament are 83.68%, 78.46% and 73.79%, respectively. The control medicament of 55 g/mu of 6% kasugamycin wettable powder and 70 g/mu of 20% bronopol wettable powder has the average control effect of 77.70% and 73.69% respectively after the treatment of the last medicament for 7 days.
The control effect 7 days after the last drug is subjected to biological statistical analysis by adopting a Duncan's New negative range (DMRT) method, and the variance analysis result shows that the difference among treatments reaches an extremely significant level and the difference among repetitions is not significant. The difference comparison result shows that the difference significance is consistent with the comparison result on the level of 0.05 and the level of 0.01. The difference between 35 g/mu of the test medicament 30% kasugamycin-bronopol water dispersible granule and other treatments reaches an extremely significant level; the treatment difference between 30 g/mu of the test medicament 30% kasugamycin-bronopol water dispersible granule and 55 g/mu of the control medicament 6% kasugamycin wettable powder is not obvious, but the difference between the test medicament and the other treatments reaches a remarkable level; the difference between 25 g/mu of treatment of the test medicament 30% kasugamycin-bronopol water dispersible granule and 70 g/mu of treatment of the control medicament 20% bronopol wettable powder is not obvious.
In conclusion, the test medicament 30% kasugamycin-bronopol water dispersible granules have good prevention and treatment effects on the bacterial angular leaf spot of the melon, the average prevention and treatment effect of 25-35 g/mu 7 days after the last medicament reaches about 74-84%, and the safety is good, so that the occurrence of the bacterial angular leaf spot of the melon can be effectively controlled.
Application example five: EXAMPLE 3 efficacy test for controlling bacterial angular leaf spot of melon
The third application example is referred to as specific experimental basis, experimental medicament and dosage, investigation method and calculation method.
1 test site
Taigu county of shanxi province.
2 test crops, varieties and growth conditions
The melon is new sweet and has good growth condition.
3 method of administering a drug
3.1 application periods and methods
Testing the first application of the drug in 2018, 5 month and 28 days; applying the medicine for the second time in 6 months and 4 days; the third application is carried out on 11 days after 6 months, and the total application is carried out 3 times.
3.2 Capacity of use
The amount of the liquid medicine sprayed per hectare is about 675 liters.
3.3 investigation time
First survey: the first application is carried out in 2018, 5, 28 and the field shows sporadic scabs, and the disease index is recorded as zero.
And (4) second investigation: the control effect is investigated 7 days after the last drug application in 2018, 6 and 18 days, and the disease occurrence condition is recorded according to the grading standard.
4 results and analysis
TABLE 5 test results of 30% kasugamycin and bronopol water dispersible granule for preventing and treating melon bacterial angular leaf spot
The results of the control effect survey 7 days after the last application of the example 3 show that when the active ingredients of the 30 percent kasugamycin-bronopol water dispersible granule of the test agent are 112.5 g/hectare, 135 g/hectare and 157.5 g/hectare, the control effects are 73.83 percent, 81.36 percent and 85.71 percent respectively; when the dosage of the active ingredient of the 6 percent kasugamycin wettable powder of the contrast medicament is 49.5 g/hectare, the control effect is 80.72 percent; when the dosage of the active ingredient of the 20 percent bronopol wettable powder of the contrast agent is 210 g/hectare, the control effect is 81.21 percent.
The test result is analyzed by adopting a DMRT method, and the test medicament 30 percent kasugamycin-bronopol water dispersible granule has obvious control effect difference between the high concentration and other treatments no matter on the level of 0.05 or 0.01; the difference between the control effects of the medium concentration of the test medicament and the control medicament 6% kasugamycin wettable powder and 20% metronidazole wettable powder is not significant; the difference between the low concentration of the test medicament and the control medicament 6 percent kasugamycin wettable powder and 20 percent bronopol wettable powder is very obvious.
The 30 percent kasugamycin-bronopol water dispersible granule is promoted and used in production to apply the pesticide before or at the early stage of the onset of the bacterial angular leaf spot of the melon, the pesticide application times are 3 times at intervals of 7 to 10 days, and the dosage of the active ingredients is 112.5 to 135 grams per hectare (the dosage of the preparation is 25 to 30 grams per mu) for spray control. The test dosage of the test reagent is safe to melon plants and fruits.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, numerous simple deductions or substitutions may be made without departing from the spirit of the invention, which shall be deemed to belong to the scope of the invention.
Claims (9)
1. The application of the bactericidal composition in preventing and treating the bacterial angular leaf spot of the melon is characterized in that: the effective active ingredients of the bactericidal composition are kasugamycin and bronopol, wherein the weight ratio of the kasugamycin to the bronopol is 9.
2. Use according to claim 1, characterized in that: the bactericidal composition contains kasugamycin, bronopol and an auxiliary agent and is prepared into water dispersible granules.
3. Use according to claim 1 or 2, characterized in that: the weight ratio of kasugamycin to bronopol is 1 to 3 to 1.
4. Use according to claim 3, characterized in that: the weight ratio of kasugamycin to bronopol is 1.
5. Use according to any one of claims 1 to 4, characterized in that: the total weight of the kasugamycin and the bronopol in the bactericidal composition is 10-60%.
6. Use according to claim 5, characterized in that: the total weight of the kasugamycin and the bronopol in the bactericidal composition is 20-40%.
7. Use according to claim 6, characterized in that: the total weight of the kasugamycin and the bronopol in the bactericidal composition is 30 percent.
8. Use according to claim 2, characterized in that: the auxiliary agent is selected from one or more of dispersing agent, wetting agent, binder, defoaming agent, pH regulator and filler.
9. Use according to any one of claims 1 to 8, characterized in that: the dosage of the active ingredients of the sterilization composition is 112.5 to 157.5 grams per hectare.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211086325.7A CN115316393A (en) | 2022-09-06 | 2022-09-06 | Application of bactericidal composition in preventing and treating bacterial angular leaf spot of melons |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211086325.7A CN115316393A (en) | 2022-09-06 | 2022-09-06 | Application of bactericidal composition in preventing and treating bacterial angular leaf spot of melons |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115316393A true CN115316393A (en) | 2022-11-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211086325.7A Pending CN115316393A (en) | 2022-09-06 | 2022-09-06 | Application of bactericidal composition in preventing and treating bacterial angular leaf spot of melons |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115316393A (en) |
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2022
- 2022-09-06 CN CN202211086325.7A patent/CN115316393A/en active Pending
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