CN115886016B - Composition containing berberine - Google Patents
Composition containing berberine Download PDFInfo
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- CN115886016B CN115886016B CN202211578920.2A CN202211578920A CN115886016B CN 115886016 B CN115886016 B CN 115886016B CN 202211578920 A CN202211578920 A CN 202211578920A CN 115886016 B CN115886016 B CN 115886016B
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- CN
- China
- Prior art keywords
- berberine
- sodium
- sodium benzoate
- moss
- disease
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 229940093265 berberine Drugs 0.000 title claims abstract description 37
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 239000000203 mixture Substances 0.000 title claims abstract description 11
- 241000207199 Citrus Species 0.000 claims abstract description 17
- 235000020971 citrus fruits Nutrition 0.000 claims abstract description 17
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 17
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 17
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 17
- 238000013329 compounding Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 16
- ITCAUAYQCALGGV-XTICBAGASA-M sodium;(1r,4ar,4br,10ar)-1,4a-dimethyl-7-propan-2-yl-2,3,4,4b,5,6,10,10a-octahydrophenanthrene-1-carboxylate Chemical compound [Na+].C([C@@H]12)CC(C(C)C)=CC1=CC[C@@H]1[C@]2(C)CCC[C@@]1(C)C([O-])=O ITCAUAYQCALGGV-XTICBAGASA-M 0.000 abstract description 15
- 239000000575 pesticide Substances 0.000 abstract description 7
- 241000195649 Chlorella <Chlorellales> Species 0.000 abstract description 6
- 230000001717 pathogenic effect Effects 0.000 abstract description 6
- 241000195493 Cryptophyta Species 0.000 abstract description 5
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 230000002195 synergetic effect Effects 0.000 abstract description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 18
- 239000003814 drug Substances 0.000 description 9
- 238000011835 investigation Methods 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 230000002265 prevention Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000002420 orchard Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OICGFSUWXCJLCO-UHFFFAOYSA-N 1-ethylsulfonylsulfanylethane Chemical compound CCSS(=O)(=O)CC OICGFSUWXCJLCO-UHFFFAOYSA-N 0.000 description 1
- 241000133570 Berberidaceae Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 241000196319 Chlorophyceae Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 239000005760 Difenoconazole Substances 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- 239000005802 Mancozeb Substances 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000001700 mitochondrial membrane Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a berberine-containing composition. A composition containing berberine comprises berberine and sodium benzoate or sodium rosinate as effective components. When the berberine and sodium benzoate or sodium abietate are compounded, the berberine and sodium benzoate or sodium abietate have good synergistic effect on the fictive chlorella, can improve the control effect on citrus moss, can reduce the application dosage of pesticides, and can delay the generation of drug resistance of pathogenic algae.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a berberine-containing composition.
Background
In citrus orchards, it is often seen that trunks, branches, leaves and fruits, even on the ground, are covered with a layer of green "clothes", which is moss disease, the etiological agent of which is the fictive chlorella (Apatococclus lobatus) of the genus fictive chlorella of the family Chlorophyceae. Moss is mainly damaged in the middle and lower parts of the crown, and the whole plant is damaged when serious, so that photosynthesis of the tree body and appearance and quality of fruits are seriously affected. The production is usually controlled by adopting bactericides such as mancozeb, difenoconazole, iprodione or ethylicin, and the like, and the pathogenic substances have drug resistance with long-term use of the medicines, so that the control effect is not ideal.
Berberine is also called berberine, is a quaternary alkaloid of phenylpropanoid, and has a molecular formula of C 20H18NO4, is a yellow needle-like crystal, has a melting point of 145 ℃, is soluble in water, and is insoluble in benzene, diethyl ether and chloroform. Is contained in a plurality of plants such as berberidaceae. Since it has a broad-spectrum antibacterial effect, it has been found in recent years that berberine can effectively prevent and treat moss diseases by destroying the integrity of cell walls, cytoplasmic membranes and mitochondrial membranes, and destroying DNA to cause death of fungal cells. However, if berberine is singly applied for a long time to prevent and treat citrus moss, drug resistance is easy to be generated, and the prevention effect of berberine is reduced.
The inventor researches that the common method for developing new pesticides is to compound different chemical pesticides, and the inventor researches that the berberine and sodium benzoate or sodium abietate are compounded according to a certain mass ratio to show obvious synergistic effect when the citrus moss is prevented and treated, and no related report is found at present.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a berberine-containing composition which can improve the control of citrus moss and delay the generation of drug resistance of pathogenic algae.
In order to achieve the above purpose, the present invention provides the following technical solutions:
The first aim of the invention is to provide a composition containing berberine, the active ingredients of which are compounded by berberine and sodium benzoate or sodium abietate.
Preferably, the mass ratio of the berberine to the sodium benzoate is 1-10:40-1.
Preferably, the mass ratio of the berberine to the sodium abietate is 1-5:15-1.
A second object of the present invention is to provide the use of the above composition for controlling citrus moss.
Compared with the prior art, the invention has the following beneficial effects:
When the berberine and sodium benzoate or sodium abietate are compounded, the berberine and sodium benzoate or sodium abietate have good synergistic effect on the fictive chlorella, can improve the control effect on citrus moss, can reduce the application dosage of pesticides, and can delay the generation of drug resistance of pathogenic algae.
Detailed Description
The following detailed description of specific embodiments of the invention is, but it should be understood that the invention is not limited to specific embodiments.
The field efficacy: after berberine is compounded, the effect of preventing and treating citrus moss disease
1. Test agent
98% Of berberine raw medicine (Shanghai Ala Biotechnology Co., ltd.), 99% of sodium benzoate raw medicine (Jiangxi Huayuyuan Biotechnology Co., ltd.), 45% of sodium abietate wettable powder (Zhejiang Dapeng pharmaceutical Co., ltd.).
After the raw materials are prepared into single-dose mother liquor, a plurality of groups of proportions are arranged, and each single-dose mother liquor and the proportion mixed liquor are diluted by 0.1 percent of Tween-80 until the mass concentration of active ingredients is 30mg/L for standby.
2. Test method (refer to pesticide field efficacy test criterion (II) GB/T17980.98-2004: bactericide for preventing and treating mango anthracnose).
In the Nanfeng orange orchard of the Guangxi Liuzhou Kangan county, the orange trees with consistent growth vigor, plant depression and common occurrence of orange moss disease are selected, and the identified pathogen is algae of the Chlorella genus of Chlorophyta.
The experiment set up multiple treatments, 3 replicates each, 2 fruit trees each. And (3) carrying out full-plant spraying test medicaments on all the treated citrus trees by adopting a spraying method in the moss disease occurrence period, so as to uniformly spray the front and back sides of citrus branches and leaves without dropping liquid medicine, and setting the spraying of equal amount of clear water as a blank control.
The test was run for 3 total disease surveys: the 1 st investigation is the investigation before the first application, and the common attack of the district plants is investigated; the 2 nd investigation was a 7d investigation after the first application; the 3 rd investigation was a 14d after the first administration investigation. 5 directions in the north and south of the east are investigated for each tree, 10 leaves are investigated for each direction, the total leaves and the leaf numbers of all stages of diseases are recorded and investigated, and the disease index and the prevention and treatment effect of each treatment are calculated.
3. Leaf disease classification table
See table 1.
TABLE 1 leaf disease Condition grading Table
| Progression of illness state | Leaf spot area |
| 0 | Leaf blade without disease spot |
| 1 | The area of the disease spots accounts for less than 10 percent of the area of the blade |
| 3 | The area of the disease spots accounts for 11-25% of the area of the leaf blade |
| 5 | The area of the disease spots accounts for 26-50% of the area of the leaf blade |
| 7 | The area of the disease spots accounts for 51-75% of the area of the leaf blade |
| 9 | The area of the disease spots accounts for more than 75 percent of the area of the blade |
4. Calculation formula
5. Test results
See tables 2-3.
Table 2 effect of berberine and sodium benzoate on preventing and treating citrus moss disease
From table 2, it can be seen that after 7d of application, berberine, sodium benzoate and different proportions of the berberine and sodium benzoate gradually show a certain control effect on citrus moss, and the control effect after 14d of application is better than that after 7 d. When the medicine is applied for 14d, the prevention effect of the compound treatment of berberine and sodium benzoate is 71.19-83.74%.
Table 3 control effect of berberine and sodium abietate on citrus moss disease
From Table 3, it can be seen that after 7d of the drug application, berberine, sodium abietate and different proportions of the berberine and sodium abietate gradually show a certain control effect on citrus moss, and the control effect after 14d of the drug application is better than that after 7 d. When the medicine is applied for 14d, the prevention effect of the compound treatment of the berberine and the sodium abietate is 68.84-86.93%, and the prevention effect of the independent treatment of the berberine and the sodium abietate is lower than 68.84%.
In conclusion, after the berberine and sodium benzoate or sodium abietate are compounded, the berberine and sodium benzoate or sodium abietate are synergistic in a certain mass ratio range to the citrus moss pathogenic algae species fictive chlorella, so that the control effect on the citrus moss can be improved.
Claims (2)
1. The berberine-containing composition is characterized in that the effective components of the composition are prepared by binary compounding of berberine and sodium benzoate, and the mass ratio of the berberine to the sodium benzoate is 1-10:40-1.
2. Use of the composition according to claim 1 for controlling citrus moss.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211578920.2A CN115886016B (en) | 2022-12-07 | 2022-12-07 | Composition containing berberine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211578920.2A CN115886016B (en) | 2022-12-07 | 2022-12-07 | Composition containing berberine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115886016A CN115886016A (en) | 2023-04-04 |
| CN115886016B true CN115886016B (en) | 2024-04-30 |
Family
ID=86489503
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211578920.2A Active CN115886016B (en) | 2022-12-07 | 2022-12-07 | Composition containing berberine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115886016B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961019A (en) * | 2010-10-29 | 2011-02-02 | 湖北太极生化开发有限公司 | Plant source bactericide and application thereof |
| CN102792975A (en) * | 2012-08-14 | 2012-11-28 | 安徽巨地农业科技有限公司 | Taste-type bird and mouse repellent composition |
| CN105994311A (en) * | 2016-06-22 | 2016-10-12 | 昆明百事德生物化学科技有限公司 | Botanical pesticide capable of controlling tobacco bacterial wilt and preparation method of botanical pesticide |
| CN111264523A (en) * | 2020-03-19 | 2020-06-12 | 成都新朝阳作物科学股份有限公司 | Application of DDAB in preparation of medicines for preventing and treating moss and moss preventing and treating composition |
| CN112715544A (en) * | 2021-01-27 | 2021-04-30 | 山东科太药业有限公司 | Pesticide composition for preventing and treating plant powdery mildew |
| CN114145305A (en) * | 2021-12-24 | 2022-03-08 | 广西壮族自治区农业科学院 | Composition for preventing and treating citrus moss |
-
2022
- 2022-12-07 CN CN202211578920.2A patent/CN115886016B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961019A (en) * | 2010-10-29 | 2011-02-02 | 湖北太极生化开发有限公司 | Plant source bactericide and application thereof |
| CN102792975A (en) * | 2012-08-14 | 2012-11-28 | 安徽巨地农业科技有限公司 | Taste-type bird and mouse repellent composition |
| CN105994311A (en) * | 2016-06-22 | 2016-10-12 | 昆明百事德生物化学科技有限公司 | Botanical pesticide capable of controlling tobacco bacterial wilt and preparation method of botanical pesticide |
| CN111264523A (en) * | 2020-03-19 | 2020-06-12 | 成都新朝阳作物科学股份有限公司 | Application of DDAB in preparation of medicines for preventing and treating moss and moss preventing and treating composition |
| CN112715544A (en) * | 2021-01-27 | 2021-04-30 | 山东科太药业有限公司 | Pesticide composition for preventing and treating plant powdery mildew |
| CN114145305A (en) * | 2021-12-24 | 2022-03-08 | 广西壮族自治区农业科学院 | Composition for preventing and treating citrus moss |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115886016A (en) | 2023-04-04 |
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