LU501128B1 - Novel Biopesticide Method for Efficiently Controlling Asparagus Stem Blight - Google Patents
Novel Biopesticide Method for Efficiently Controlling Asparagus Stem Blight Download PDFInfo
- Publication number
- LU501128B1 LU501128B1 LU501128A LU501128A LU501128B1 LU 501128 B1 LU501128 B1 LU 501128B1 LU 501128 A LU501128 A LU 501128A LU 501128 A LU501128 A LU 501128A LU 501128 B1 LU501128 B1 LU 501128B1
- Authority
- LU
- Luxembourg
- Prior art keywords
- parts
- chinese herbal
- stem blight
- asparagus stem
- carbendazim
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
Abstract
The invention discloses a novel environmental-friendly biological pesticide which can effectively control asparagus stem blight, and belongs to the technical field of agricultural biological control. The novel biopesticide consists of the following raw materials in parts by weight: 10-20 parts of chitosan oligosaccharide, 20-40 parts of active extracts of Chinese herbal medicines, 10-40 parts of carbendazim, 8-15 parts of surfactant and 100- 200 parts of water. Through the synergistic effect of chitosan oligosaccharide, carbendazim and active extracts of Chinese herbal medicines, the invention can significantly control asparagus stem blight and improve Asparagus production capacity;the new bio-pesticide is safe, green and pollution-free, which is beneficial to the sustainable development of Asparagus planting industry.
Description
DESCRIPTION Novel Biopesticide Method for Efficiently Controlling Asparagus Stem Blight
TECHNICAL FIELD The invention relates to a novel biopesticide method for efficiently controlling asparagus stem blight, in particular to a biopesticide for efficiently controlling asparagus stem blight prepared by comprehensively utilizing chitosan oligosaccharide, Chinese herbal medicine extract and carbendazim, belonging to the technical field of agricultural biological control.
BACKGROUND Asparagus officinalis, also known as Asparagus, 1s a perennial herb of Asparagus (genus) in Asparagus, Asparagaceae. Its young plants can be used for vegetables, and it has the reputation of "the king of vegetables" in the international market. Asparagus has high nutritional value, can moisten lung, relieve cough, eliminate phlegm and inhibit tumor growth, which is very popular among people. In recent years, with the expansion of Asparagus cultivation area, the occurrence of diseases is increasing year by year, especially the occurrence and harm of stem blight, which seriously affects the yield and quality of Asparagus. The pathogen of asparagus stem blight is Phomopsis asparagi, which is a worldwide devastating disease. The disease spreads rapidly in rainy and windy conditions, and it can also be spread by rain splashing. Air infection is the main cause of large-scale disease. The direction of field spread and rapid disease are often affected by wind. In addition, low-lying, sticky soil, excessive nitrogen fertilizer, etc., are easy to aggravate the disease. The disease is serious in Asian Asparagus planting countries such as China, Japan, Thailand, Indonesia, etc., especially in China, which is common in all Asparagus producing provinces in China, and the south is more important than the north. Light results in poor growth and development of Asparagus, which reduces the yield and quality; the seriously ill plants die in advance, and the whole field is destroyed. At present, the control of asparagus stem blight is mainly chemical control. However, due to the extensive use of chemical agents for many years, the pathogen showed strong drug resistance in many areas, which greatly reduced the effect of pesticides. Lin Peili et al. and Kang Yebin tested the toxicity of different pesticides to asparagus stem blight. In the case of Peng Mingsheng and Xiao Feng, the control effects of 60% carbendazim hydrochloride wettable powder, 70% thiophanate-methyl wettable powder, 50% benomyl wettable powder, 50% Captafol wettable powder and 12.5% diniconazole wettable powder were determined. However, these fungicides are all fungicides with a long service life, and asparagus stem blight is easy to be resistant to these fungicides, so it is particularly important to find alternative fungicides to control stem blight.
Patent 2017105568113 discloses a new type of biological pesticide for preventing and treating asparagus stem blight and asparagus brown spot. Its main components are Poly(hexamethylenebiguanide)hydrochloride, zineb and thiophanate methyl. It can be seen that the patented technology is still dominated by chemical control; Patent 2013103015752 discloses a chemical fertilizer for preventing and treating asparagus stem blight and its fertilization method. The key technology lies in adjusting the ratio of nitrogen, phosphorus and potassium of chemical fertilizer to keep the soil acidic and destroy the disease condition of asparagus stem blight, which is a temporary solution. To sum up, in the prior art, the control of asparagus stem blight is mainly based on chemical agents, and the new biological agents are mainly used to prevent the occurrence of asparagus stem blight, but the control effect on the already-occurring asparagus stem blight is relatively small, thus easily causing excessive pesticide residues, affecting food safety and reducing Asparagus production benefits.
SUMMARY In order to make up for the defects of the prior art, the invention provides a novel biological pesticide for efficiently preventing and controlling asparagus stem blight, which can significantly prevent and control asparagus stem blight through the synergistic effect of chitosan oligosaccharide, active extracts of Chinese herbal medicines, carbendazim and other raw materials, and improve the yield and quality of Asparagus, thereby improving the planting benefit of Asparagus; the new bio-pesticide is safe, green and pollution-free, which is beneficial to the sustainable development of Asparagus planting industry.
To achieve the above purpose, the technical scheme adopted by the invention is as follows:
a novel biopesticide for efficiently controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 10-20 parts of chitosan oligosaccharide, 20- 40 parts of active extracts of Chinese herbal medicines, 10-40 parts of carbendazim, 8-15 parts of surfactant and 100-200 parts of water.
Preferably, as mentioned above, a novel biopesticide for effectively preventing and controlling asparagus stem blight 1s prepared from the following raw materials in parts by weight: 12-16 parts of chitosan oligosaccharide, 25-32 parts of active extract of Chinese herbal medicine, 20-30 parts of carbendazim, 8-15 parts of surfactant and 100-200 parts of water.
More preferably, as mentioned above, a novel biological pesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 15 parts of chitosan oligosaccharide, 30 parts of active extract of Chinese herbal medicine, 25 parts of carbendazim, 10 parts of surfactant and 150 parts of water.
The molecular weight of chitosan oligosaccharide is 1,000-2,000 Da, among which chitosan oligosaccharide with molecular weight of 1,500 Da 1s the best.
The carbendazim 1s 50% wettable powder.
The active extracts of Chinese herbal medicines are water extracts of Peucedanum praeruptorum dunn, cordate houttuynia. and liquorice, wherein the compatibility ratio of Chinese herbal medicines 1s 25 parts of Peucedanum praeruptorum dunn, 18 parts of cordate houttuynia. and 7 parts of liquorice.
The active extract of Chinese herbal medicine is prepared by the following methods: crushing Chinese herbal medicine raw materials with impurities removed, sieving with a 100-mesh sieve, adding purified water 5 times of Chinese herbal medicine raw materials, adding 5% cellulase and 3% xylanase in the mass ratio of Chinese herbal medicine raw materials, and performing enzymolysis at 50-60°C for 12 h under the condition of ultrasonic assistance; after enzymolysis, filtering, and the filtrate is the active extract of Chinese herbal medicine.
The invention also provides a preparation method of a novel biological pesticide for efficiently preventing and controlling asparagus stem blight, which comprises the following steps:
1) weighing chitosan oligosaccharide, carbendazim, active extracts of Chinese herbal medicines, surfactants and water in a formula amount; 2) putting the chitosan oligosaccharide, carbendazim and surfactant weighed in step 1) into the water of the formula amount, and stirring until they are completely dissolved; 3) putting the active extracts of Chinese herbal medicines in the formula amount into the solution prepared in step 2), and stirring evenly to obtain a novel biological pesticide for effectively preventing and controlling asparagus stem blight.
In the present invention, chitosan oligosaccharide are easy to penetrate through the bacterial cell membrane and easily enter the cytoplasm and nucleus, so that the related enzymes with physiological activities inside the cells are leaked, and at the same time, they interact with negatively charged substances such as DNA to interfere with the replication and transcription of DNA, thus exerting their antimicrobial activity; chitosan oligosaccharide can also induce the disease resistance of plants, and can immunize and kill a variety of fungi, bacteria and viruses.
The active extract of Chinese herbal medicine plays an important role in promoting the invention, and the antibacterial, bactericidal and disease prevention effects of the product of the invention are significantly improved by functional active substances such as saponins in the active ingredients of Chinese herbal medicine; in addition, it is found that the combined use of chitosan oligosaccharide, carbendazim and active extracts of Chinese herbal medicines has a strong sustained- release function and prolongs the action time of the product.
Carbendazim is a broad-spectrum fungicide, which can prevent and control diseases caused by fungi (such as fungi imperfecti and polyspora fungi) in many crops, and can be used for foliar spraying, seed treatment and soil treatment, etc.
The existing research results show that carbendazim has poor control effect on asparagus stem blight, and excessive application can produce drug resistance.
By compounding chitosan oligosaccharide, active extract of Chinese herbal medicine and carbendazim, it is found that carbendazim can quickly inhibit the incidence of asparagus stem blight and reduce the harm of asparagus stem blight to Asparagus production.
The invention further provides an application method of the novel bio-pesticide for efficiently preventing and controlling asparagus stem blight in Asparagus planting, which is characterized in that the novel bio-pesticide for efficiently preventing and controlling asparagus stem blight is diluted into 200-300 times of water for spraying, and the usage amount is 500-1,000 g/hm?.
Compared with the prior art, the invention has the following beneficial effects: (1) through the synergistic effect of chitosan oligosaccharide, carbendazim and active extracts of Chinese herbal medicines, the invention improves the efficacy of preventing asparagus stem blight, so that the product has a remarkable efficacy of preventing asparagus stem blight, changes the technical defects of preventing asparagus stem blight by using chemical agents or changing the growth conditions of bitter bamboo shoots in the prior art, reduces the residues of traditional Chinese medicines in the Asparagus planting process, has a wide application range, has a remarkable yield-increasing effect, and can significantly improve the food safety and planting benefits of Asparagus; (2) the product of the invention is environment-friendly, and the selected main raw materials are chitosan oligosaccharide and active ingredients of Chinese herbal medicines, thus causing less secondary pollution to the agricultural growth environment; (3) according to the invention, the quick sterilization carbendazim, chitosan oligosaccharide with long-acting bacteriostasis and active ingredients of Chinese herbal medicines are combined for use, so that not only can the effect of quickly inhibiting stem blight be exerted, but also the recurrence of stem blight can be inhibited for a long time; (4) the present invention mainly contains chitosan oligosaccharide, carbendazim and active extracts of Chinese herbal medicines, which act together and have obvious synergistic effect, so that the novel biological pesticide of the present invention has remarkable effects in preventing and controlling asparagus stem blight and improving Asparagus yield and quality, and has achieved the technical effect of "1+1>2"; (5) the raw materials of the active extract of Chinese herbal medicine of the present invention are Peucedanum praeruptorum dunn, cordate houttuynia. and liquorice, wherein Peucedanum praeruptorum dunn is used for dispelling wind heat, cordate houttuynia is used for clearing away heat and toxic materials, and liquorice is used for blending various medicines; the experimental results show that the single Chinese herbal medicine has no significant efficacy in preventing asparagus stem blight, and it can play a certain role in preventing asparagus stem blight after the reasonable compatibility of
Peucedanum praeruptorum dunn , cordate houttuynia and liquorice. More importantly, it can significantly promote the efficacy of the product in preventing asparagus stem blight.
DESCRIPTION OF THE INVENTION In the following embodiments, the active extracts of Chinese herbal medicines are all prepared by the following processes: crushing Chinese herbal medicines with impurities removed, sieving with a 100-mesh sieve, adding purified water 5 times as much as Chinese herbal medicines, adding 5% cellulase and 3% xylanase in the mass ratio of Chinese herbal medicines, and carrying out enzymolysis at 50-60°C for 12 h under the condition of ultrasonic assistance; after enzymolysis, filtering, and the filtrate is the active extract of Chinese herbal medicine. In the following embodiments, the molecular weight of chitosan oligosaccharide 1s around 1,500 Da; the carbendazim mentioned above is 50% wettable powder. Embodiment 1 A novel biopesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 15 parts of chitosan oligosaccharide, 30 parts of active extracts of Chinese herbal medicines, 25 parts of carbendazim, 10 parts of surfactant and 150 parts of water. The preparation method of the novel biopesticide for effectively preventing and controlling asparagus stem blight comprises the following steps: 1) weighing chitosan oligosaccharide, carbendazim, active extracts of Chinese herbal medicines, surfactants and water in a formula amount; 2) putting the chitosan oligosaccharide, carbendazim and surfactant weighed in step 1) into the water of the formula amount, and stir until they are completely dissolved; 3) putting the active extracts of Chinese herbal medicines in the formula amount into the solution prepared in step 2), and uniformly stirring to obtain a novel biological pesticide for efficiently preventing and treating asparagus stem blight. The new bio-pesticide with high efficiency for controlling asparagus stem blight is diluted into 300-fold solution with water and sprayed, and the dosage was 600 g/hm>. Embodiment 2 A novel biopesticide for effectively preventing and treating asparagus stem blight is prepared from the following raw materials in parts by weight: 16 parts of chitosan oligosaccharide, 32 parts of active extract of Chinese herbal medicine, 30 parts of carbendazim, 15 parts of surfactant and 200 parts of water.
The preparation method and application method are the same as those in Embodiment 1. Embodiment 3 A novel biopesticide for effectively preventing and controlling asparagus stem blight 1s prepared from the following raw materials in parts by weight: 12 parts of chitosan oligosaccharide, 25 parts of active extracts of Chinese herbal medicines, 20 parts of carbendazim, 8 parts of surfactant and 100 parts of water.
The preparation method and application method are the same as those in Embodiment 1. Comparative Embodiment 1 (the new biopesticide contains only active extracts of Chinese herbal medicines, and the rest 1s the same as Embodiment 1) A novel biological pesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 70 parts of active extract of Chinese herbal medicine, 10 parts of surfactant and 150 parts of water.
The preparation method and application method are the same as those in Embodiment 1. Comparative Embodiment 2 (the new biopesticide only contains chitosan oligosaccharide, the rest is the same as Embodiment 1) A novel biopesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 70 parts of chitosan oligosaccharide, 10 parts of surfactant and 150 parts of water; The preparation method and application method are the same as those in Embodiment 1. Comparative Embodiment 3 (the new biopesticide only contains carbendazim, the rest 1s the same as Embodiment 1) A novel biopesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 70 parts of carbendazim, parts of surfactant and 150 parts of water.
The preparation method and application method are the same as those in Embodiment 1; Comparative Embodiment 4 (the new biopesticide contains no active extracts of Chinese herbal medicines, and the rest is the same as in Embodiment 1)
A novel biopesticide for controlling asparagus stem blight efficiently is prepared from the following raw materials in parts by weight: 15 parts of chitosan oligosaccharide, 25 parts of carbendazim, 10 parts of surfactant and 180 parts of water. The preparation method and application method are the same as those in Embodiment 1; Comparative Embodiment 5 (the raw materials of Chinese herbal medicine in new biopesticide only contain Peucedanum praeruptorum dunn, the rest is the same as in Embodiment 1) A novel biopesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 15 parts of chitosan oligosaccharide, 30 parts of active extracts of Chinese herbal medicines, 25 parts of carbendazim, 10 parts of surfactant and 150 parts of water. The preparation method and application method are the same as those in Embodiment 1; Comparative Embodiment 6 (the raw material of Chinese herbal medicine in the new biopesticide only contains cordate houttuynia, and the rest is the same as in Embodiment 1) A novel biopesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 15 parts of chitosan oligosaccharide, 30 parts of active extracts of Chinese herbal medicines, 25 parts of carbendazim, 10 parts of surfactant and 150 parts of water. The preparation method and application method are the same as those in Embodiment 1; Comparative Embodiment 7 (the raw materials of Chinese herbal medicine in the new biopesticide only contain licorice, the rest is the same as in Embodiment 1) A novel biopesticide for effectively preventing and controlling asparagus stem blight is prepared from the following raw materials in parts by weight: 15 parts of chitosan oligosaccharide, 30 parts of active extracts of Chinese herbal medicines, 25 parts of carbendazim, 10 parts of surfactant and 150 parts of water. The preparation method and application method are the same as those in Example 1. Embodiment 4 compares the effects of different new biological pesticides on the control effect of asparagus stem blight and yield increase index
1. Disease investigation
Sampling at five diagonal points in each plot, taking 5 connected Asparagus clusters at each point, randomly investigating 3 Asparagus clusters in each cluster, totally investigating 25 Asparagus clusters and 75 Asparagus clusters, and recording the number of healthy plants and the number of diseased plants at all levels. The grading standard of illness refers to the standard formulated by Yang Yingqing, etc. Grade 0: no disease; Grade 1: the incidence area accounts for less than 6% of the total area; Grade 2: the incidence area accounts for 6%-10% of the total area; Grade 3: the incidence area accounts for 11%-20% of the total area; Grade 4: the incidence area accounts for 21%- 30% of the total area; Grade 5: the incidence area accounts for more than 30% of the total area,
2. Test method Experiment with the experimental method recorded in "Control Effect of Six Fungicides on asparagus stem blight" by Yang Yingqing and Lan Bo.
3. Data processing and calculation method Disease index= Y (number of diseased plants at all levels * relative level value) / total number of investigated plants * 5 * 100; Control effect=(disease index of control area-disease index of treatment area)/disease index of control area* 100%; Yield increase rate=(yield of treatment area-yield of blank control area)/yield of blank control area* 100%;
4. Experimental results Table 1 Effects of different new biological pesticides on stem blight control and yield increase index index Embodiment 1 oT | meee | wee Comparative 27.45+1.09 11.45+0.94 Embodiment 1
Group of Comparative 25.37+1.38 9.57+0.87 Embodiment 2 Group of Comparative 19.91+1.26 8.74+1.15 Embodiment 3 Group of Comparative 48.36+1.16 12.92+1.44 Embodiment 4 Group of Comparative 55.29+1.41 13.08+1.23 Embodiment 5 Group of Comparative 58.80+1.13 13.18+1.09 Embodiment 6 Group of Comparative 52.17+0.95 12.86+1.27 Embodiment 7 As shown in Table 1, the test results show that, compared with the conventional operation of the control group, the control effects of Embodiments 1-3 on asparagus stem blight are all above 82%, which shows that the product of the invention has remarkable control effects on asparagus stem blight Comparative Embodiment 1 contains only active extracts of Chinese herbal medicines, and its maximum control effect on asparagus stem blight 1s 27.46%, which shows that active extracts of Chinese herbal medicines have a certain control effect, but the effect is not significant.
Comparative Embodiment 2 contains only chitosan oligosaccharide, but does not contain active extracts of Chinese herbal medicines and carbendazim, and its control effect on stem blight is 25.37%, but the difference is significant compared with Embodiments 1-3. The control Embodiment 3 contains only carbendazim, but does not contain active extracts of Chinese herbal medicines and chitosan oligosaccharide.
Its control effect on stem blight is 19.91%, but the difference is significant compared with Embodiments 1-3. Comparing the control effects of Comparative Embodiments 1-3 on asparagus stem blight, it shows that chitosan oligosaccharide, carbendazim and active extracts of Chinese herbal medicines have obvious synergistic effect, and the technical effect of "1+1>2" has been achieved in the control of asparagus stem blight.
At the same time, the experimental results also show that the product of the invention has significant effect on increasing Asparagus yield, and changing the raw material composition of the product will significantly reduce the growth promoting effect of the product (P<0.01). By comparison, it is determined that Embodiment 1 is the best example of the present invention.
The influence of the composition of Chinese herbal medicine raw materials on the control effect of biological pesticide asparagus stem blight was analyzed. The experimental results of Comparative Embodiments 4-7 showed that changing the composition of Chinese herbal medicine raw materials would significantly reduce the control effect of biological pesticide asparagus stem blight. Although Peucedanum praeruptorum dunn, cordate houttuynia and liquorice had a certain synergistic effect on enhancing the control effect of biological pesticide, the synergistic effect among Peucedanum praeruptorum dunn, cordate houttuynia and liquorice was the fundamental reason for the control of asparagus stem blight by Chinese herbal medicine raw materials.
The above embodiments are only used to illustrate the technical scheme of the present invention, but not to limit it; although the invention has been described in detail with reference to the foregoing embodiments, it is still possible for ordinary technicians in the field to modify the technical scheme described in the foregoing embodiments or to replace some of its technical features equivalently. However, these modifications or substitutions do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions claimed by the present invention.
Claims (9)
1. A novel biopesticide for efficiently controlling asparagus stem blight, which is characterized by being prepared from the following raw materials in parts by weight: 10- parts of chitosan oligosaccharide, 20-40 parts of active extract of Chinese herbal medicine, 10-40 parts of carbendazim, 8-15 parts of surfactant and 100-200 parts of water.
2. The novel biopesticide for effectively preventing and controlling asparagus stem blight according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 12-16 parts of chitosan oligosaccharide, 25-32 parts of Chinese herbal medicine active extract, 20-30 parts of carbendazim, 8-15 parts of surfactant and 100-200 parts of water.
3. The novel biopesticide for effectively preventing and controlling asparagus stem blight according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 15 parts of chitosan oligosaccharide, 30 parts of active extracts of Chinese herbal medicines, 25 parts of carbendazim, 10 parts of surfactant and 150 parts of water.
4. The novel biopesticide for effectively preventing and controlling asparagus stem blight according to claims 1-3, characterized in that the molecular weight of chitosan oligosaccharide is 1,000-2,000 Da, among which chitosan oligosaccharide with molecular weight of 1,500 Da are the best.
5. The novel biopesticide for effectively preventing and controlling asparagus stem blight according to claims 1-3, characterized in that carbendazim is 50% wettable powder.
6. The novel biopesticide for effectively preventing and controlling asparagus stem blight according to claims 1-3, characterized in that the active extract of Chinese herbal medicine is the water extract of Peucedanum praeruptorum dunn, cordate houttuynia and liquorice, wherein the compatibility ratio of Chinese herbal medicine raw materials is 25 parts of Peucedanum praeruptorum dunn, 18 parts of cordate houttuynia and 7 parts of liquorice.
7. The novel biopesticide for effectively preventing and controlling asparagus stem blight according to claim 6, characterized in that the active extract of Chinese herbal medicine is prepared by the following methods: crushing Chinese herbal medicine raw materials with impurities removed, sieving with a 100-mesh sieve, adding purified water 5 times as much as Chinese herbal medicine raw materials, adding 5% cellulase and 3% xylanase in the mass ratio of Chinese herbal medicine raw materials, and carrying out enzymolysis at 50-60°C for 12 h under the condition of ultrasonic assistance; after enzymolysis, filtering, and the filtrate is the active extract of Chinese herbal medicine.
8. The novel biopesticide for effectively preventing and controlling asparagus stem blight according to claim 1, characterized in that the preparation process comprises the following steps: 1) weighing chitosan oligosaccharide, carbendazim, active extracts of Chinese herbal medicines, surfactants and water in a formula amount; 2) putting the chitosan oligosaccharide, carbendazim and surfactant weighed in step 1) into the water of the formula amount, and stir until they are completely dissolved; 3) putting the active extracts of Chinese herbal medicines in the formula amount into the solution prepared in step 2), and stirring evenly to obtain a novel biological pesticide for effectively preventing and controlling asparagus stem blight.
9. The novel bio-pesticide for controlling asparagus stem blight efficiently according to claim 1, characterized in that the novel bio-pesticide for controlling asparagus stem blight efficiently is diluted with 200-300 times of water and sprayed with 500-1,000 g/hm?.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU501128A LU501128B1 (en) | 2021-12-30 | 2021-12-30 | Novel Biopesticide Method for Efficiently Controlling Asparagus Stem Blight |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU501128A LU501128B1 (en) | 2021-12-30 | 2021-12-30 | Novel Biopesticide Method for Efficiently Controlling Asparagus Stem Blight |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LU501128B1 true LU501128B1 (en) | 2022-06-30 |
Family
ID=82272156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LU501128A LU501128B1 (en) | 2021-12-30 | 2021-12-30 | Novel Biopesticide Method for Efficiently Controlling Asparagus Stem Blight |
Country Status (1)
| Country | Link |
|---|---|
| LU (1) | LU501128B1 (en) |
-
2021
- 2021-12-30 LU LU501128A patent/LU501128B1/en active IP Right Grant
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101697736B (en) | Bacillus subtilis and propiconazole compound wettable sterilizing powder and use thereof | |
| US20060228428A1 (en) | A composition for preventing plant diseases resulted from infection of plant pathogens and a method for preparing the same | |
| DE102007004500B4 (en) | Use of a formulation containing Sapindus extract for the treatment of a plant disease supported or caused by fungi and / or oomycetes | |
| CN110074113B (en) | Application of stropharia rugoso-annulata polysaccharide, plant immunity inducer and application | |
| KR20130092275A (en) | Method for manufacturing sulfer fertilizer and sulfer fertilizer thereof | |
| CN109497099B (en) | Novel biopesticide for efficiently preventing and treating stem blight of asparagus | |
| CN102217661B (en) | Botanical bactericide and preparation method and application thereof | |
| CN111066831A (en) | Chinese herbal medicine source pesticide | |
| CN104920514A (en) | Traditional Chinese medicine bactericide for peanuts | |
| LU501128B1 (en) | Novel Biopesticide Method for Efficiently Controlling Asparagus Stem Blight | |
| LU504685B1 (en) | Biological pesticide for preventing and controlling asparagus stem blight | |
| CN111406753B (en) | Pharmaceutical composition for preventing and treating peanut stripe virus disease and application thereof | |
| CN114158406A (en) | Citrus minor blight and citrus black blight control method and application thereof | |
| CN109452319B (en) | Composition for efficiently preventing and treating stem blight of asparagus and application thereof | |
| CN102511509A (en) | Method for extracting allelochemical for resisting Mikania micrantha from Heteropanaxfragrans leaves | |
| CN109090156B (en) | Preparation method of konjac soft rot control medicine prepared from scutellaria baicalensis and control method thereof | |
| CN109452324A (en) | A kind of composite biological pesticidal preparations for preventing and treating tea tree anthracnose | |
| CN111587883A (en) | Plant pesticide and preparation method and use method thereof | |
| CN110477037B (en) | Plant source pesticide composition and preparation method and application thereof | |
| CN104642406A (en) | Pesticide extract for preventing and treating corm rottenness pathogen of saffron crocus, and usage method | |
| CN112998017B (en) | Application of composition containing kasugamycin and zinc thiazole in preventing and treating soft rot of dendrobium officinale | |
| CN116158449B (en) | Radix linderae extract herbicide and application thereof | |
| CN115399345B (en) | Composition containing paris polyphylla extract and preparation method and application thereof | |
| CN113615455B (en) | Green bletilla striata rust prevention and control method | |
| CN114982787B (en) | Composition for preventing and treating asparagus stem blight and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Patent granted |
Effective date: 20220630 |