CN111406753B - Pharmaceutical composition for preventing and treating peanut stripe virus disease and application thereof - Google Patents
Pharmaceutical composition for preventing and treating peanut stripe virus disease and application thereof Download PDFInfo
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- CN111406753B CN111406753B CN202010400643.0A CN202010400643A CN111406753B CN 111406753 B CN111406753 B CN 111406753B CN 202010400643 A CN202010400643 A CN 202010400643A CN 111406753 B CN111406753 B CN 111406753B
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- brassinolide
- ningnanmycin
- pharmaceutical composition
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- peanut
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
- A01N45/02—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring having three carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
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- Life Sciences & Earth Sciences (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Environmental Sciences (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a pharmaceutical composition for preventing and treating peanut stripe virus diseases and application thereof, and belongs to the technical field of pesticide compounding. The active ingredients of the pharmaceutical composition for preventing and treating the peanut stripe virus disease are brassinolide and ningnanmycin; the concentration ratio of brassinolide to ningnanmycin in the medicinal composition is 0.1-0.3: 80. The brassinolide and ningnanmycin compound in the pharmaceutical composition has better prevention effect on PStV than that of a single agent, and has obvious promotion effect on growth and yield of peanuts.
Description
Technical Field
The invention belongs to the technical field of pesticide compounding, and particularly relates to a pharmaceutical composition for preventing and treating peanut stripe virus diseases and application thereof.
Background
Peanut Stripe virus (PStV) is widely prevalent in Peanut producing areas in China, is the most serious in northern planting areas, and has the most serious influence on the Peanut industry in China in virus diseases. The popular years are in Hebei and Beijing in North China, Henan in China, Shaanxi in northwest China, Liaoning in northeast China, peanut planting areas in Shandong, Anhui, Jiangsu and other provinces (cities) in east China, the common planting areas have more than 50 percent of peanut plants, and some plots or even all plots have diseases, so that the yield reduction of peanuts is serious and the quality is reduced. The prevention and treatment of peanut stripe virus mostly adopts a method of planting disease-resistant varieties, and effective prevention and treatment measures are lacked. At present, the natural product antiviral preparation has more researches on resisting virus diseases of solanaceae crops, but reports on application of the natural product antiviral preparation to peanut virus diseases are not found.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a pharmaceutical composition for preventing and treating peanut stripe virus diseases and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a pharmaceutical composition for preventing and treating peanut stripe virus disease contains brassinolide and ningnanmycin as effective components; the concentration ratio of brassinolide to ningnanmycin in the medicinal composition is 0.1-0.3: 80.
On the basis of the scheme, the concentration ratio of the brassinolide to the ningnanmycin in the pharmaceutical composition is 0.3: 80.
The pharmaceutical composition is applied to preventing and treating peanut stripe virus diseases, promoting peanut growth and improving peanut yield.
A method for preventing and treating stripe virus disease of peanut, promoting peanut growth, improving peanut output at the same time, add water and dilute the above-mentioned pharmaceutical composition until the brassinolide concentration is 0.1-0.3 mg/L, the concentration of ningnanmycin is 80 mg/L; and uniformly spraying peanut plants.
The technical scheme of the invention has the advantages
The PStV control effect (45.0%) of the combination of Brassinolide and Ningnanmycin in the pharmaceutical composition for preventing and treating peanut stripe virus diseases is higher than that of the combination of single-dose Brassinolide (36.1%) and Ningnanmycin (34.5%), the promotion effect on peanut growth and yield is obvious, and the improvement of the flower yield (35.6%) of the combination of the Brassinolide and the Ningnanmycin (21.2%) is better than that of the combination of single-dose Brassinolide (13.8%) and the Ningnanmycin.
Detailed Description
Terms used in the present invention have generally meanings as commonly understood by one of ordinary skill in the art, unless otherwise specified.
The present invention will be described in further detail with reference to the following data, with reference to specific examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.
Example 1
A pharmaceutical composition for preventing and treating peanut stripe virus disease contains brassinolide and ningnanmycin as effective components; the concentration of brassinolide in the medicinal composition is 0.1mg/L, the concentration of ningnanmycin is 80mg/L, and the balance is water.
Example 2
A pharmaceutical composition for preventing and treating peanut stripe virus disease contains brassinolide and ningnanmycin as effective components; the concentration of brassinolide in the medicinal composition is 0.2mg/L, the concentration of ningnanmycin is 80mg/L, and the balance is water.
Example 3
A pharmaceutical composition for preventing and treating peanut stripe virus disease contains brassinolide and ningnanmycin as effective components; the concentration of brassinolide in the medicinal composition is 0.3mg/L, the concentration of ningnanmycin is 80mg/L, and the balance is water.
Test of drug efficacy
The test is arranged at a Laxi test station of peanut research institute in Shandong province, the selected plot peanuts are continuously planted for 12 years, the PStV diseases are serious in the past year, and the disease incidence rate in 2017 and 2018 reaches more than 85%. Adopting ridge culture and film covering for planting, the ridge distance is 85cm, the ridge surface width is 55cm, the ridge ascending distance is 30cm, the hole distance is 15.7cm, and the peanut density is 15 ten thousand holes/hm22 seeds per hole are cultivated in a mulching film.
Medicament: 0.01% brassinolide (limited by Henan Zhen Yu Biotech), 8% Ningnanmycin aqua (Deqiang biological products Co., Ltd.), Ningnanmycin-brassinolide built-up agent (prepared by the laboratory). Peanut variety: huayu No. 20 is preserved by Shandong province peanut research institute
The test is totally provided with 8 treatments, the concentration of the active ingredients of each treatment group is shown in table 1, and the active ingredients of each group are original drugs diluted to specific concentration by adding water; wherein, the concentration of ningnanmycin is 80mg/L, and 3 concentrations of brassinolide are set: 0.1mg/L, 0.2mg/L, 0.3 mg/L; the ningnanmycin is respectively combined with 3 concentrations of brassinolide, each treatment is repeated for 4 times, clear water is used as blank control, random block arrangement is carried out, and the cell surfaceProduct 21.25m2The planting method and field management are the same as those of a production field.
The brassinolide single agent, the ningnanmycin single agent and the compound agent are uniformly sprayed on peanut plants by an electric sprayer for 10 kg/mu, sowed in 5 months and 2 days, and applied for 2 days in 6 months, 17 days in 6 months and 2 days in 7 months respectively for 3 times in total. And (5) investigating the disease condition 14 days after the last pesticide application, and counting the prevention and treatment effect.
TABLE 1 Components and concentrations of the treatments
And (3) determining disease indexes: and randomly surveying 20 peanuts in each cell 14 days after the last pesticide application, counting the morbidity and disease index, and calculating the prevention and treatment effect. The disease index was graded as shown in Table 2.
TABLE 2 grading Standard of PStV
The drug effect calculation method comprises the following steps:
during harvesting, 20 plants are randomly selected for each treatment, and the main stem height, the lateral branch length, the branch number of each plant, the pod number of each plant and the plumpness rate are investigated, and the yield is calculated after the pods are dried in the sun and indoor seed test. Excel and sps software is used for carrying out variance analysis on the prevention and treatment effect of the virus diseases, and a Duncan's new repolarization method is used for carrying out difference significance test.
PStV prevention and treatment effect of single agent and compound agent
TABLE 3 preventive and therapeutic effects on PStV by Single and Compound Agents
Data listed are the average of 4 replicates, and different letters are assigned to those with significant differences (P ═ 0.05) by square difference analysis.
As can be seen from Table 3, both the brassinolide and the ningnanmycin have certain control effects on PStV, and after the two medicaments are compounded according to a certain proportion, the control effects are obviously better than those of a single medicament, wherein the treatment effect of V is the best, the control effect can reach 45.0 percent, and the control effect is better than that of the single medicament of brassinolide (0.3mg/L, the control effect is 36.1 percent) and that of the ningnanmycin (80mg/L, the control effect is 34.5 percent), which shows that the compounding of the two medicaments has a synergistic effect. The brassinolide has the best control effect at the concentration of 0.3mg/L no matter whether a single agent or a compound agent is used (Table 3).
Effect of Single and Compound Agents on peanut growth
TABLE 4 Effect of Single and Complex Agents on flower production
Data listed are the average of 4 replicates, and different letters are assigned to those with significant differences (P ═ 0.05) by square difference analysis.
As can be seen from Table 4, the treatment with brassinolide and ningnanmycin single or compound agent is better than the control in the stem height, the side branch length and the branch number; the number of single plant fruits, the number of full fruits, the weight of all kernels, the kernel yield and the yield are all obviously higher than those of the control. The yield of the brassinolide and the ningnanmycin which are singly used are increased, wherein the brassinolide (0.3mg/L) and the ningnanmycin (80mg/L) are respectively increased by 13.8 percent and 21.2 percent compared with a control; the yield increasing effect of the treatment V is best, and the yield is increased by 35.6%. The compound preparation is shown to have synergistic effect on the control effect and the yield of the peanut virus (Table 4).
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (3)
1. The application of a pharmaceutical composition in preventing and treating peanut stripe virus disease; the active ingredients of the pharmaceutical composition are brassinolide and ningnanmycin; the concentration ratio of brassinolide to ningnanmycin in the medicinal composition is 0.1-0.3: 80.
2. The use according to claim 1, wherein the concentration ratio of brassinolide to ningnanmycin in the pharmaceutical composition is 0.3: 80.
3. A method for preventing and treating peanut stripe virus diseases, which is characterized in that the pharmaceutical composition applied in the claim 1 or 2 is diluted by water until the concentration of brassinolide is 0.1-0.3 mg/L and the concentration of ningnanmycin is 80 mg/L; and uniformly spraying peanut plants.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010400643.0A CN111406753B (en) | 2020-05-13 | 2020-05-13 | Pharmaceutical composition for preventing and treating peanut stripe virus disease and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010400643.0A CN111406753B (en) | 2020-05-13 | 2020-05-13 | Pharmaceutical composition for preventing and treating peanut stripe virus disease and application thereof |
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| CN111406753A CN111406753A (en) | 2020-07-14 |
| CN111406753B true CN111406753B (en) | 2021-07-13 |
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| CN114176089B (en) * | 2021-12-23 | 2023-06-27 | 中国科学院昆明植物研究所 | Pesticide composition for resisting Chinese rose stem tumor and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109938027A (en) * | 2019-04-03 | 2019-06-28 | 江西鑫邦科技有限责任公司 | A kind of composition pesticide of anti-plant viral disease and its preparation method and application |
| CN110896947A (en) * | 2019-12-16 | 2020-03-24 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating plant virus diseases and preparation method and application thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109938027A (en) * | 2019-04-03 | 2019-06-28 | 江西鑫邦科技有限责任公司 | A kind of composition pesticide of anti-plant viral disease and its preparation method and application |
| CN110896947A (en) * | 2019-12-16 | 2020-03-24 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating plant virus diseases and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 抗烟草花叶病毒病的药剂筛选及复配增效作用研究;伍小良;《西南大学硕士论文集》;20070630;摘要、第16-19页 * |
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