CN111264523B

CN111264523B - Application of DDAB in preparation of medicines for preventing and treating moss and moss preventing and treating composition

Info

Publication number: CN111264523B
Application number: CN202010196406.7A
Authority: CN
Inventors: 何其明; 任丹; 牟天秀; 杜波; 何洁; 陆桂芝; 廖立群; 赵文娟
Original assignee: Chengdu New Chaoyang Crop Science Co ltd
Current assignee: Chengdu New Chaoyang Crop Science Co ltd
Priority date: 2020-03-19
Filing date: 2020-03-19
Publication date: 2021-11-19
Anticipated expiration: 2040-03-19
Also published as: CN111264523A

Abstract

The invention relates to application of DDAB in preparation of a moss preventing and treating medicine and a moss preventing and treating composition, and belongs to the technical field of pesticides. Application of DDAB in preparing medicines for preventing and treating moss is provided. The moss preventing and treating composition comprises: DDAB and berberine, wherein the weight ratio of DDAB to berberine is 1-16: 0.4-0.6. The invention uses DDAB for preventing and treating the green moss of fruit trees, and provides a new choice for preventing and treating the green moss. The invention also combines berberine and DDAB according to a specific proportion to obtain a novel moss preventing and treating composition, and the novel moss preventing and treating composition can be adopted for moss which has drug resistance to the existing moss preventing and treating medicaments.

Description

Application of DDAB in preparation of medicines for preventing and treating moss and moss preventing and treating composition

Technical Field

The invention relates to application of DDAB in preparation of a moss preventing and treating medicine and a moss preventing and treating composition, and belongs to the technical field of pesticides.

Background

Citrus is a large product in global fruit production and has a very important position in the economic trade of fruits in the world.

The citrus industry has become an important characteristic industry for accelerating the structure adjustment of the agricultural industry and promoting the development of rural areas in south China and the income increase of farmers in mountain areas.

Generally, the high-temperature rainy, warm and humid climate is favorable for the growth of citrus, but simultaneously provides a 'hotbed' for the propagation and growth of various pathogens, and diseases are easy to occur; and the citrus is an evergreen fruit tree, has luxuriant branches and leaves throughout the year and high tree body canopy density, and provides favorable conditions for the occurrence of citrus diseases. The citrus lichen disease is a disease which is seriously influenced by humid and rainy climate. In rainy seasons in south China, particularly in citrus orchards with poor ventilation, a layer of green clothes is often covered on trunks, branches and leaves, and even on the ground, and the green moss is the disease.

Currently, moss damaging oranges is mainly of the following two types: the green algae is attached to the green algae, the green algae is mainly adsorbed on the trunk, the branches and part of old leaves of a plant, and a layer of grass green algae is arranged on the damaged plant organ; secondly, the moss is a green plant which is more or less spotted in most orchards and is also a common moss in citrus gardens, and besides harms citrus plants, the moss also can harm soil in the orchards so as to reduce the air permeability of the moss. The moss on the tree body is usually generated on the ground, branches and leaves, and the photosynthesis of plants is weakened, so that the tree body is poor in growth, the tree vigor is reduced and the like, and the yield and the quality of the citrus are seriously affected.

DDAB, didodecyldimethylammonium bromide, is known in the art as a cationic surfactant, catalyst, emulsifier, disinfectant, germicide, antistatic agent, and the like.

Berberine, also known as berberine, is an isoquinoline alkaloid distributed in many plants. It is an important active and effective component of Chinese medicinal material coptis in the aspect of pharmacy for the first time, and can inhibit inflammation, bacterial and viral infection, etc.

Disclosure of Invention

The invention aims to solve the first problem of providing the application of DDAB in preparing a medicine for preventing and treating moss.

Preferably, the moss is moss on a fruit tree, and more preferably moss on a citrus tree.

Preferably, the content of DDAB in the medicine is 1-80 wt%, the content of DDAB is preferably 5-16 wt%, and the content of DDAB is more preferably 10-13 wt%.

When the DDAB is used, the DDAB is diluted to an effective concentration, the concentration of the DDAB in the medicine is high, the times of dilution are large, the concentration of the DDAB in the medicine is low, and the times of dilution are small.

Preferably, the medicine also contains 1-40 wt% of ethanol, preferably 1-30 wt%, and the balance of water and inevitable impurities.

Preferably, the medicament also contains berberine, and the weight ratio of DDAB to berberine is as follows: 1-16: 0.4-0.6; preferably 5-16: 0.4-0.6; more preferably 5 to 10:0.4 to 0.6.

Preferably, the medicine also contains fatty alcohol-polyoxyethylene ether, sodium dioctyl sulfosuccinate, ethanol, and the balance of water and inevitable impurities; the weight ratio of DDAB, fatty alcohol-polyoxyethylene ether, sodium dioctyl sulfosuccinate and ethanol is as follows: 1-16: 0.5-5: 2-20: 1-30; preferably 5-13: 0.5-5: 2-20: 1-30; more preferably 5 to 10:0.5 to 5:2 to 20:1 to 30.

Further, the application also comprises diluting the medicine to DDAB concentration of 0.033-0.085 wt% in use, preferably to 0.05-0.085 wt%; when the medicine also contains berberine, the medicine is more preferably diluted to DDAB concentration of 0.033-0.06 wt%.

The invention aims to solve the second technical problem of providing a moss preventing and treating composition.

To solve the second technical problem of the present invention, the composition comprises: DDAB and berberine, wherein the weight ratio of DDAB to berberine is 1-16: 0.4-0.6; preferably, the weight ratio of DDAB to berberine is 5-16: 0.4-0.6; more preferably, the weight ratio of DDAB to berberine is 5-10: 0.4-0.6.

The berberine can be berberine and its salt, and when berberine is berberine salt, such as berberine sulfate. The weight ratio of DDAB to berberine is 1-16: 0.4-0.6, wherein the weight of berberine is the weight of original berberine without salt.

Preferably, the composition further comprises: fatty alcohol-polyoxyethylene ether, sodium dioctyl sulfosuccinate and ethanol; the weight ratio of DDAB, fatty alcohol-polyoxyethylene ether, sodium dioctyl sulfosuccinate and ethanol is as follows: 1-16: 0.5-5: 2-20: 1-30; preferably 5-13: 0.5-5: 2-20: 1-30; more preferably 5 to 10:0.5 to 5:2 to 20:1 to 30.

Preferably, the berberine is at least one of berberine sulfate and berberine hydrochloride.

Has the advantages that:

1. the invention uses DDAB for preventing and treating the green moss of fruit trees, provides a new choice for preventing and treating the green moss, and can adopt the new green moss preventing and treating agent for the green moss which has drug resistance to the existing green moss preventing and treating agent.

2. The invention also combines berberine and DDAB according to a specific proportion to obtain a novel moss preventing and treating composition, and the novel moss preventing and treating composition can be adopted for moss which has drug resistance to the existing moss preventing and treating medicaments.

Drawings

FIGS. 1 to 7 are photographs of experiments of treatments Nos. 1 to 7 in Table 4 of example 2, respectively.

The 1 st column from the left in the figure is a photograph of the experiment at day 0; column 2 is a photograph of experiment day 2; column 3 is a photograph of 4 days of the experiment; column 4 is a photograph of the experiment at day 6.

The same row in the figure represents a photograph of the same leaf.

Detailed Description

Preferably, the composition further comprises: fatty alcohol-polyoxyethylene ether, dioctyl sodium sulfosuccinate and ethanol; the weight ratio of DDAB, fatty alcohol-polyoxyethylene ether, sodium dioctyl sulfosuccinate and ethanol is as follows: 1-16: 0.5-5: 2-20: 1-30; preferably 5-13: 0.5-5: 2-20: 1-30; more preferably 5 to 10:0.5 to 5:2 to 20:1 to 30.

The following examples are provided to further illustrate the embodiments of the present invention and are not intended to limit the scope of the present invention.

The experimental methods of the examples and comparative examples of the present invention are as follows:

(1) experimental methods

The spraying method is adopted. The test agent is prepared according to the experimental design and then is evenly sprayed on the ground of each leaf. 1.5L hand-pressed watering cans are used for the tree trunks and the leaves, the spraying amount is consistent for each treatment, and the spraying amount is up to the moss layer.

(2) Investigation time and method

1 tree was selected for each treatment prior to the trial and 10 leaves with consistent disease were marked.

Recording the incidence of moss on each treated leaf before the test, and photographing and recording each treated leaf; and continuously observing the death condition of each processed moss every day after the test, photographing and recording, carrying out disease grade statistics 2 days, 4 days and 6 days after the test, and grading according to the following standards.

(3) Grading standard:

level 0: no moss exists;

level 1: the moss area accounts for 0-10% of the whole leaf area;

and 3, level: the moss area accounts for 10 to 25 percent of the whole leaf area;

and 5, stage: the moss area accounts for 26-50% of the whole leaf area;

and 7, stage: the moss area accounts for 51 to 75 percent of the whole leaf area;

and 9, stage: the moss area accounts for more than 75% of the whole leaf area.

After investigation, the control effect is calculated according to the following formula:

disease index (∑ (number of leaves at each stage × number of relative stages)/total number of leaves investigated × 9) × 100

Control effect (%) (no base before administration) (1- (treatment zone disease index/CK disease index)) × 100. Before application, there is no base number, namely the control effect relative to the clear water control group.

Medicament:

70% of original berberine adopted in the experiment of the invention is berberine sulfate with the berberine content of 70 wt%; 80% DDAB is an ethanol solution with a DDAB content of 80 wt%.

Example 1:

the experimental site: tan Tugoucun of Yanggu lake town of Pianjiang county, Sichuan province

Subject: and (5) carrying out moss on citrus leaves. Variety: spring meeting; the age of the tree: the 23-year-old high-quality culture medium is improved, and the 6 th year of the variety appears in spring;

experiment time: 2019-10-16 to 2019-10-22.

Example 1 the experimental agents are detailed in table 1.

Table 1 example 1 experimental dosage table

The results are detailed in table 2.

Table 2 table of experimental results of example 1

And (4) experimental conclusion: no obvious effect appears 2 days after the application, and the prevention effect appears 4 days after the application.

0.49% berberine aqueous single dose: the difference of the control effects of 150 times and 200 times of dilution is not large, and the control effects are about 30 percent

Single dose of DDAB: the prevention and treatment effect is improved along with the increase of the concentration, wherein the effect can reach more than 70% when the concentration is 16%;

the dilution of 80% of DDAB 6.25% + 70% of berberine and 80% of DDAB 12.5% + 70% of berberine by 150 times is obviously synergistic compared with a single dose, but the improvement of DDAB concentration is not obvious in synergistic effect compared with the single dose, but the better technical scheme is the scheme of 80% of DDAB 6.25% + 70% of berberine by combining the cost and the dilution times, namely 5% of pure DDAB + 0.49% of pure berberine.

Example 2:

the experimental site: wulong village in the city of the great county, cattail, Sichuan province

Subject: citrus, variety: spring age: 5 to 6 years old

Test time: 2020-01-13 to 2020-01-19.

Example 2 experimental agents are detailed in table 3.

Table 3 example 2 experimental dosage form

The results are detailed in table 4.

Table 4 table of experimental results of example 2

And (4) experimental conclusion: compared with the application of 80% DDAB 6.25% + 70% berberine diluted 150 times with 0.7% and 80% DDAB 6.25% single agent diluted 150 times with 70% berberine single agent diluted 150 times, the composition has obvious synergistic effect.

Claims

The application of DDAB in preparing medicines for preventing and treating moss; the medicine also contains berberine, and the weight ratio of DDAB to berberine is as follows: 1-16: 0.4-0.6; the moss is moss on citrus trees.
2. The use of a DDAB as claimed in claim 1, in the manufacture of a medicament for the prevention or treatment of moss, wherein the DDAB content of the medicament is from 1 to 80 wt%.
3. The use of a DDAB as claimed in claim 2, in the manufacture of a medicament for the prevention or treatment of moss, wherein the DDAB content of the medicament is from 5 to 16 wt%.
4. The use of a DDAB as claimed in claim 2, in the manufacture of a medicament for the prevention or treatment of moss, wherein the DDAB content of the medicament is from 10 to 13 wt%.
5. The use of a DDAB as claimed in claim 2, in the manufacture of a medicament for the prevention or treatment of moss, wherein the medicament further comprises 1 to 40 wt% ethanol, the balance being water and unavoidable impurities.
6. The use of a DDAB as claimed in claim 5, to prepare a medicament for the prevention or treatment of moss, wherein the medicament further comprises 1 to 30 wt% ethanol.
7. The use of a DDAB as claimed in claim 1 or 2 in the manufacture of a medicament for the prevention or treatment of moss, wherein the DDAB and berberine are present in the following weight ratios: 5-16: 0.4-0.6.
8. The use of a DDAB as claimed in claim 7 in the manufacture of a medicament for the prevention or treatment of moss, wherein the DDAB and berberine are present in the following weight ratios: 5-10: 0.4-0.6.
9. The use of a DDAB as defined in claim 2 in the manufacture of a medicament for the prevention or treatment of moss, wherein the medicament further comprises fatty alcohol polyoxyethylene ether, dioctyl sodium sulfosuccinate, ethanol, and the balance water and unavoidable impurities; the weight ratio of DDAB, fatty alcohol-polyoxyethylene ether, sodium dioctyl sulfosuccinate and ethanol is as follows: 1-16: 0.5-5: 2-20: 1-30.
10. The use of a DDAB as defined in claim 9 to prepare a medicament for the prevention or treatment of moss, wherein the DDAB, fatty alcohol polyoxyethylene ether, dioctyl sodium sulfosuccinate, and ethanol are present in the following weight ratios: 5-13: 0.5-5: 2-20: 1-30.
11. The use of a DDAB as defined in claim 9 to prepare a medicament for the prevention or treatment of moss, wherein the DDAB, fatty alcohol polyoxyethylene ether, dioctyl sodium sulfosuccinate, and ethanol are present in the following weight ratios: 5-10: 0.5-5: 2-20: 1-30.
12. Use of a DDAB as claimed in claim 2, in the manufacture of a medicament for the control of moss, wherein the use further comprises diluting the medicament to a DDAB concentration of 0.033 to 0.085% by weight, when in use.
13. Use of a DDAB as claimed in claim 2, in the manufacture of a medicament for the control of moss, further comprising diluting the medicament to a DDAB concentration of 0.05 to 0.085 wt% in use.
14. The use of a DDAB as claimed in claim 2, in the preparation of a medicament for the prevention or treatment of moss, wherein when the medicament further comprises berberine, the medicament is diluted to a DDAB concentration of 0.033 to 0.06 wt%.
15. A moss controlling composition, the composition comprising: DDAB and berberine, wherein the weight ratio of DDAB to berberine is 1-16: 0.4-0.6.
16. The moss control composition of claim 15, wherein the composition includes: DDAB and berberine, wherein the weight ratio of DDAB to berberine is 5-16: 0.4-0.6.
17. The moss control composition of claim 15, wherein the composition includes: DDAB and berberine, wherein the weight ratio of DDAB to berberine is 5-10: 0.4-0.6.
18. The moss control composition of any one of claims 15 to 17, wherein the composition further comprises: fatty alcohol-polyoxyethylene ether, dioctyl sodium sulfosuccinate and ethanol; the weight ratio of DDAB, fatty alcohol-polyoxyethylene ether, sodium dioctyl sulfosuccinate and ethanol is as follows: 1-16: 0.5-5: 2-20: 1-30.
19. The moss preventing and treating composition of claim 18, wherein the weight ratio of DDAB, fatty alcohol-polyoxyethylene ether, dioctyl sodium sulfosuccinate, and ethanol is: 5-13: 0.5-5: 2-20: 1-30.
20. The moss preventing and treating composition of claim 18, wherein the weight ratio of DDAB, fatty alcohol-polyoxyethylene ether, dioctyl sodium sulfosuccinate, and ethanol is: 5-10: 0.5-5: 2-20: 1-30.
21. The moss preventing and treating composition of claim 18, wherein the berberine is at least one of berberine sulfate and berberine hydrochloride.