CN115812549A - Method for rapidly recovering ophthalmia glabra population - Google Patents

Method for rapidly recovering ophthalmia glabra population Download PDF

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CN115812549A
CN115812549A CN202211455284.4A CN202211455284A CN115812549A CN 115812549 A CN115812549 A CN 115812549A CN 202211455284 A CN202211455284 A CN 202211455284A CN 115812549 A CN115812549 A CN 115812549A
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population
water body
propagule
water
glabra
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潘俊峰
程中平
刘艳玲
廖廓
杨东
操瑜
文香英
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Wuhan Botanical Garden of CAS
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Wuhan Botanical Garden of CAS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention discloses a method for rapidly recovering ophthalmia glabra population, which comprises the following steps: s1, breeding a propagule bank, including propagule treatment, planting, breeding management and the like; s2, seed throwing of a propagule bank, including seed throwing conditions, plant pruning, seed throwing density and the like; s3, maintenance management, including water level control, water temperature adjustment, pest control and the like; and S4, monitoring population and controlling biological quantity. The invention can ensure that the ophthalmia glabra population has obvious recovery effect, high initial survival rate and short seedling recovery time, and the population is stably amplified in the later period, so that the ophthalmia glabra population can be quickly recovered, and the ecological recovery of a water body is promoted. The method is easy to implement, simple and convenient to operate, low in cost, quick in effect and good in popularization.

Description

Method for rapidly recovering ophthalmia glabra population
Technical Field
The invention relates to a submerged plant community restoration and reconstruction technology. More particularly, the present invention relates to a method for rapidly recovering a phoma longifolia population.
Background
Submerged plants are the most basic and important primary producers of water ecosystem, play the bait, inhabit and breed the place for part of fishes, benthonic animals, zooplankton and the like in the water body, and have important ecological significance in maintaining the structural stability and species diversity of the water ecosystem. In addition, the submerged plant also plays an irreplaceable role in the aspects of water quality purification, landscape improvement, improvement of water habitat conditions and the like. However, due to the enhancement of human activities, water body pollution such as the acceleration of eutrophication process, the rapid decrease of transparency and the like is caused, submerged plants are degenerated and disappear, and a series of water ecological environment problems such as water quality deterioration, frequent occurrence of water bloom, water ecosystem damage and the like are caused. Therefore, the restoration and reconstruction of submerged plant communities are considered to be one of the important means for the damaged water ecosystem to carry out water body restoration and biodiversity restoration.
The selection of the restoring species is one of the most major technical problems in restoration and reconstruction of submerged plant communities. Potamogeton lucens Morong is a perennial submerged herb of Potamogeton genus of Potamogeton family of ophthalmocaceae, and is distributed in irrigation canals, ponds, rivers and the like in provinces of northeast, east and west of China and in Yunnan equal places, and belongs to native submerged plants in China. The potamogeton glabra has a rhizome, the shape of the rhizome is similar to that of a root, the tuber grows below a soil layer, obvious nodes and internodes are formed, the nodes have redifferentiation capacity, and terminal buds are formed. The top bud or node of the rhizome generates root system, and new plant individual is cloned and generated. The rhizomes store abundant nutrients and can survive for one to many years. Therefore, the potamogeton phocarpus is an excellent water ecological restoration pioneer species based on the biological characteristic analysis and the field distribution characteristic analysis of the potamogeton phocarpus.
The planting method is also the most important technical problem in the restoration and reconstruction of submerged plant communities. Under the general condition, the ecological balance of the damaged water body is broken, and the submerged plant community is difficult to restore through a natural mechanism, so that most of submerged plants for restoring the water environment are artificially and auxiliarily planted. The submerged plant planting methods currently used include a cutting method, a throw-out method and a sowing method. The insertion planting method is to directly insert the root base of the plant into soft substrate mud at a low water level, and has the defects of high labor intensity and operation difficulty, and limited application by water depth, substrate softness and properties. The throwing planting method is that soft clay, gauze, plastic cloth and other materials are used to tie the root base of the plant and then the plant is directly thrown and planted under the action of gravity, and the defects are that the wrapping soft clay or clay is easy to break away and run off, and the tied or wrapped plant has limitation on the plant growth, so that the plant survival rate is low and the plant growth state is poor. The direct seeding method has the disadvantages of scattering of seeds with water, lowest survival rate after germination, influence of various environmental factors on seedling growth, long recovery period and the like.
The seeds of the potamogeton glabra have thick seed coats, are not easy to germinate in a natural state, and are not easy to collect in large batches. Therefore, there is a need to develop a restoration technology of pholiota nameko population, which is low in cost, easy to operate and high in survival rate.
Disclosure of Invention
The invention aims to solve the technical problem of rapidly recovering the population of the submerged plant ophiocarpus lucidus, and aims to provide a method for recovering the population of the ophiocarpus lucidus, which has the advantages of low cost, easy operation, high survival rate and stable population amplification. The potamogeton photeinii provided by the invention has the advantages of high survival rate and short seedling recovery time, and the population is stably amplified in the later period, so that the potamogeton photeinii population in the water body can be quickly recovered, and the ecological recovery of the water body is promoted. The method has the advantages of low cost, quick response, simple operation and good popularization.
The technical scheme adopted by the invention for solving the technical problem is as follows: a method for rapidly recovering potamogeton photeinii populations comprises the following steps:
s1. Breeding propagule library
S1.1 Container and substrate: digging fresh pond sludge, and filling into a cave disk with the depth and the caliber of 5-7 cm;
s1.2 propagule treatment: selecting a robust potamogeton glabra plant, and pruning the robust potamogeton glabra plant into a branch containing at least more than 2 nodes;
s1.3, a planting method: planting the broken branches processed by the S1.2 in the hole tray processed by the S1.1, wherein the planting density is 5-8 broken branches per hole, and at least 1 section is buried in soil with the depth of more than 1cm during planting;
s2, throwing seeds in a propagule bank
S2.1 seed throwing time: culturing the propagule bank for more than 30 days; the water temperature of the seed throwing water body is lower than 15 ℃ at night;
s2.2, pruning plants: pruning to plants comprising 2-3 nodes;
s2.3 seed throwing density: the seed throwing density is 20-30 holes per square meter;
s3. Maintenance management
S3.1, water level control: the early water level is controlled to be 0.5-0.7m, and along with the growth of plants, the water level can be gradually increased to be not more than 2 times of the transparency of the water body, generally within 2 m;
s3.2, water temperature adjustment: when the temperature of the water body is above 35 ℃ in summer, the temperature of the water body is reduced, and the damage is reduced;
s3.3, avoiding animals in the water body to take food: in the recovery period of the ophthalmia glabra population, animals in the water body are prevented from eating the ophthalmia glabra population;
s4, monitoring population and controlling biomass
When the cover degree of the submerged vegetation reaches more than 40 percent, a grass type clear water body can be constructed; when the vegetation cover degree is 40% -70%, the grass-type clear water body can be maintained; and when the vegetation coverage is more than 70%, controlling the biological quantity of the pholiota nameko population.
Preferably, S1 further includes:
s1.4, avoiding animals in the water body to take food: and in the breeding period of the propagule bank, animals in the water body are prevented from eating.
Preferably, S1 further includes:
s1.5 preventing and treating hydroplania: adult insects are killed by utilizing the trap lamp, so that the insect source base number is reduced; manually removing egg masses in the peak egg laying period; catching larvae by using a net bag in a larva incubation peak period; spraying Bacillus thuringiensis or fenitrothion emulsion in larval stage.
Preferably, S1 further includes:
s1.6, spirogyra treatment: the duty is arranged and irrigated; when a small area occurs, manual salvage processing is adopted; spraying triphenyltin acetate WP when the occurrence area reaches 30%, and not draining water outwards 30 days after application.
Preferably, the S2 further includes:
s2.4, not scattering soil blocks with a propagule bank; when seeds are thrown, the seeds need to be thrown on the water surface evenly.
Preferably, the S3 further includes:
s3.4 preventing and treating hydroplania nubilalis: adult insects are killed by utilizing the trap lamp, so that the insect source base number is reduced; manually removing egg masses in the peak egg laying period; catching larvae by using a net bag in a larva incubation peak period; spraying Bacillus thuringiensis or fenitrothion emulsion in larval stage.
Preferably, the S3 further includes:
s3.5, spirogyra treatment: the duty is arranged and irrigated; when a small area occurs, manual salvage processing is adopted; spraying triphenyltin acetate WP when the occurrence area reaches 30%, and not draining water outwards 30 days after application.
Preferably, the step of performing the biological quantity control on the potamogeton lucidus population in S4 is to increase the feeding of grass carp or to manually harvest grass carp.
The invention at least comprises the following beneficial effects: the method is characterized in that the node of the rhizomes of the potamogeton lucidus has the redifferentiation capacity to carry out vegetative propagation, and the vegetative propagation has shorter sexual propagation time, and innovatively provides a technical scheme for rapidly recovering the population of the potamogeton lucidus by adopting a throw-planting vegetative propagule bank. The invention solves the defects and limitations of the prior art when the manual planting of the phoma lucidum florets is recovered, for example, the labor intensity and the planting difficulty are high and the cost is high when a method with high survival rate and short period is adopted, and the survival rate and the period are low and the period is long when a method with low labor intensity and low planting difficulty is adopted. Meanwhile, the problems that the phoma longifolia seed group is stably amplified and forms a large-area survival stable seed group in the late planting period are solved. The method is simple and easy to implement, low in cost, quick in effect, convenient to operate and good in popularization.
Compared with the prior art, the invention has the following advantages and effects:
1) The requirement on bottom mud is low, and the device is not limited by water depth;
2) The seedling recovery time is short;
3) The emergence rate of the propagule bank is high, and the survival rate of the seedlings is high;
4) The plant growth is good, and the population is stably amplified;
5) Low labor intensity, simple and easy operation.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention will be described in detail and fully with reference to the following examples. Those skilled in the art will be able to practice the invention based on these descriptions. Before the present invention is described with reference to the following examples, it should be noted that: the technical solutions and features provided in the present invention in the respective sections including the following description may be combined with each other without conflict.
Moreover, the embodiments of the present invention described in the following description are generally only some embodiments of the present invention, and not all embodiments. Therefore, all other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.
The present invention is further described in detail with reference to the following examples and embodiments, wherein the following steps are carried out:
the invention provides a method for rapidly recovering ophthalmia glabra population, which comprises the following steps:
s1. Breeding of propagule bank
S1.1 Container and substrate: digging fresh pond sludge, and filling into a cave disk with the depth and the caliber of 5-7 cm;
s1.2 propagule treatment: selecting a robust potamogeton lucidum plant, and pruning the robust potamogeton lucidum plant into a broken branch at least comprising more than 2 nodes;
s1.3, a planting method: planting the broken branches processed by the S1.2 in the hole tray processed by the S1.1, wherein the planting density is 5-8 broken branches per hole, and at least 1 section is buried in soil with the depth of more than 1cm during planting;
s2, throwing seeds in a propagule bank
S2.1 seed throwing time: culturing the propagule bank for more than 30 days; the water temperature of the seed throwing water body is lower than 15 ℃ at night, and seeds can be thrown in the middle and lower reaches of Yangtze river basin within 5-8 months;
s2.2, pruning plants: pruning to plants comprising 2-3 nodes; the cut off branches can be used for breeding the propagule bank in the step 1.
S2.3 seed throwing density: the seed throwing density is 20-30 holes per square meter;
s3, maintenance management
S3.1, water level control: the early water level is controlled to be 0.5-0.7m, and along with the growth of plants, the water level can be gradually increased to be not more than 2 times of the transparency of the water body, generally within 2 m;
s3.2, water temperature adjustment: when the temperature of the water body is more than 35 ℃ in summer, the potamogeton lucidus is easy to cause obvious damage to the potamogeton lucidus, and the water body temperature is reduced by treatment such as sun shading or running water, so that the damage is reduced;
s3.3, avoiding animals in the water body to take food: in the recovery period of the cleome gynandra population, water animals such as fishes, shrimps and the like are prevented from eating;
s4, monitoring population and controlling biomass
When the cover degree of the submerged vegetation reaches more than 40 percent, a grass type clear water body can be constructed; when the vegetation cover degree is 40% -70%, the grass-type clear water body can be maintained; when the vegetation coverage is more than 70%, the biological quantity control of the eyebright herb population is required.
The technical scheme can also comprise the following technical details so as to better realize the technical effects: the S1 further comprises:
s1.4, avoiding animals in the water body from taking food: in the breeding period of the propagule storehouse, aquatic animals such as fishes, shrimps and the like are prevented from eating the propagule.
3. The method for rapidly recovering an ophthalmia glabra population according to claim 1 or 2, wherein the S1 further comprises:
s1.5 preventing and treating hydropathia guenee: adult insects are killed by utilizing the trap lamp, so that the insect source base number is reduced; manually removing egg masses in the peak egg laying period; catching larvae by using a net bag in a larva incubation peak period; 1500 times of Bacillus thuringiensis or 800 times of fenitrothion emulsion can be sprayed in the larval stage.
The technical scheme can also comprise the following technical details so as to better realize the technical effects: the S1 further comprises:
s1.6, spirogyra treatment: the duty is arranged and irrigated; when a small area occurs, manual salvage processing is adopted; spraying 25% of triphenyltin acetate WP when the occurrence area reaches 30%, wherein the dosage is 300-450 g/hm 2 And does not drain water to the outside 30 days after the application.
The technical scheme can also comprise the following technical details so as to better realize the technical effects: the S2 further comprises:
s2.4 notes: scattering soil blocks in the propagule bank as far as possible; when seeds are thrown, the seeds need to be thrown on the water surface evenly.
The technical scheme can also comprise the following technical details so as to better realize the technical effects: the S3 further comprises:
s3.4 preventing and treating hydroplania nubilalis: adult insects are killed by utilizing the trap lamp, so that the insect source base number is reduced; manually removing egg masses in the peak egg laying period; catching larvae by using a net bag in a larva incubation peak period; 1500 times of Bacillus thuringiensis or 800 times of fenitrothion emulsion can be sprayed in the larval stage.
The technical scheme can also comprise the following technical details so as to better realize the technical effects: the S3 further comprises:
s3.5, spirogyra treatment: the duty is arranged and irrigated; when a small area occurs, manual salvage processing is adopted; spraying 25% of triphenyltin acetate WP when the occurrence area reaches 30%, wherein the dosage is 300-450 g/hm 2 And does not drain water to the outside 30 days after the application.
The technical scheme can also comprise the following technical details so as to better realize the technical effects: in the step S4, the measures for controlling the biological quantity of the eyebright potamogeton herb population are to increase the throwing of grass carp or to harvest the grass carp artificially.
Example 1
A method for rapidly recovering ophthalmia glabra population comprises the following steps:
step 1, breeding of propagule bank
1.1 container and substrate. In middle ten days of the 5 th month in 2021, fresh pond sludge is dug and filled into a 21-hole plug with the single-hole caliber and the depth of 6.5 cm.
1.2 propagule treatment. Selecting a robust potamogeton lucidum plant, and pruning the plant to form a broken branch at least comprising more than 2 nodes.
1.3 planting method. And planting the broken branches treated by the 1.2 in the hole tray treated by the 1.1, wherein the planting density is 8 broken branches per hole, and at least 1 section is buried in soil with the depth of 1-2cm during planting.
1.4 avoid animals in the water body to take food. In the breeding period of the propagule storehouse, aquatic animals such as fishes, shrimps and the like are prevented from eating the propagule.
1.5 preventing and treating hydroplania. And (3) installing a trap lamp to trap and kill adults, spraying 1500 times of liquid of bacillus thuringiensis for 1 time to prevent and control the hydropiper, and avoiding the hazard of the hydropiper during the breeding period of the propagules.
1.6 processing with spirogyra. The labor and the logistics irrigation are adopted, the small area is salvaged, and the large area of spirogyra does not occur during the breeding period of the propagules.
Step 2, throwing seeds in a propagule bank
2.1 throwing seed time. Seed throwing at the beginning of 6 months in 2021.
2.2 pruning the plants. Cut into plants containing 2-3 nodes.
2.3 seed throwing density. The seed throwing density is 20 holes per square meter.
2.4 notes. The soil blocks with the propagule bank are prevented from being scattered as much as possible; when seeds are thrown, the seeds need to be thrown on the water surface evenly.
Step 3, maintenance management
3.1 water level control. The water level at the early stage is controlled between 0.5 m and 0.7m, and the water level is gradually increased to 1.5m along with the growth of plants.
3.2 water temperature regulation. In late 7 th to late 8 th of month, adopting a water flowing measure when the temperature of the water body is above 35 ℃, and reducing the temperature of the water body to below 35 ℃.
3.3 avoiding animals in the water body to take food. In the restoration period of the cleome gynura, water animals such as fishes, shrimps and the like are prevented from eating the cleome gynura.
3.4 preventing and treating hydroplania nubilalis. No harm of the hydroplania nubilalis occurs.
3.5 treating with spirogyra. Spraying 25% triphenyltin acetate WP with the dosage of 300g/hm 2 And does not drain water to the outside 30 days after the application.
Step 4, population monitoring and biological quantity control
When the cover degree of the submerged vegetation reaches more than 40 percent, a grass type clear water body can be constructed; when the vegetation cover degree is 40% -70%, the grass-type clear water body can be maintained; when the coverage is more than 70%, the biological quantity of the eyebright herb population must be controlled, and the grass carp feeding or manual harvesting can be properly increased.
Control group 1-bamboo pole transplanting method
In middle 5 th of 2021, the same water quality and environment was the same when the collybia glabra was planted in the adjacent pools of example 1 by bamboo-cutting planting at the same time of seed throwing. The branch breaking treatment method of S1.2 propagule treatment in the embodiment 1 is adopted, 8 branches are bundled into one bundle, and the base of the bundle is clamped by a bamboo pole until the sludge is formed, so that planting is completed. 8 branches per hole were cut by the same method as 1.3 in example 1, and the planting density was 18 holes per square meter. The remaining management method is the same as in step S3 and step S4 of embodiment 1.
Control group 2-mud wrapping throwing planting method
In the middle 5 th of the 2021 year, the potamogeton glaucophyllus is planted in the adjacent water pools of the embodiment 1 by adopting a mud wrapping and throwing planting method at the same time of throwing the seeds, and the water quality and the environment are the same. The method for treating broken branches of S1.2 propagules in example 1 is adopted, 8 broken branches are wrapped by pond sludge, and then wrapped by non-woven fabrics and directly thrown into water to finish planting. 8 branches per hole were cut by the same method as 1.3 in example 1, and the planting density was 18 holes per square meter. The remaining management method is the same as step S3 and step S4 of example 1.
After 1 month of seed throwing, the survival rate, plant height and population coverage of the euglena phocarpoides of example 1, control example 1 and control group 2 were investigated as shown in table 1. The result shows that after the method of the embodiment 1 of the invention is used for 1 month after seed polishing, the survival rate of the planted potamogeton lucidus reaches 98%, the growth height of the plant exceeds 60cm, the vegetation coverage reaches 50%, and the purpose of quickly recovering the potamogeton lucidus population is realized. The comprehensive consideration of the labor condition for recovering the water surface of unit area shows that the recovery effect of the pholiota nameko population is obviously superior to that of the bamboo pole inserting planting method and the mud wrapping throwing planting method.
TABLE 1
Figure SMS_1
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.

Claims (8)

1. A method for rapidly recovering euglena phophylla populations, comprising the steps of:
s1. Breeding of propagule bank
S1.1 Container and substrate: digging fresh pond sludge, and filling into a cave disk with the depth and the caliber of 5-7 cm;
s1.2 propagule treatment: selecting a robust potamogeton lucidum plant, and pruning the robust potamogeton lucidum plant into a broken branch at least comprising more than 2 nodes;
s1.3, a planting method: planting the broken branches processed by the S1.2 in the hole tray processed by the S1.1, wherein the planting density is 5-8 broken branches per hole, and at least 1 section is buried in soil with the depth of more than 1cm during planting;
s2, throwing seeds in a propagule bank
S2.1 seed throwing time: culturing the propagule bank for more than 30 days; the water temperature of the seed throwing water body is lower than 15 ℃ at night;
s2.2, pruning plants: pruning to plants comprising 2-3 nodes;
s2.3 seed throwing density: the seed throwing density is 20-30 holes per square meter;
s3. Maintenance management
S3.1, water level control: the early water level is controlled to be 0.5-0.7m, and the water level can be gradually increased to be not more than 2 times of the transparency of the water body along with the growth of plants;
s3.2, water temperature adjustment: when the temperature of the water body is above 35 ℃ in summer, the temperature of the water body is reduced, and the damage is reduced;
s3.3, avoiding animals in the water body to take food: in the recovery period of the ophthalmia glabra population, animals in the water body are prevented from eating the ophthalmia glabra population;
s4, monitoring population and controlling biomass
When the cover degree of the submerged vegetation reaches more than 40 percent, a grass type clear water body can be constructed; when the vegetation cover degree is 40% -70%, the grass-type clear water body can be maintained; and when the vegetation coverage is more than 70%, performing biological quantity control on the ophiocarpus lucidus population.
2. The method for rapidly recovering an ophthalmia glabra population according to claim 1, wherein the S1 further comprises:
s1.4, avoiding animals in the water body to take food: and in the breeding period of the propagule bank, animals in the water body are prevented from eating.
3. The method for rapidly recovering the phoma longifolia population according to claim 1 or 2, wherein the step S1 further comprises:
s1.5 preventing and treating hydroplania: adult insects are killed by utilizing the trap lamp, so that the insect source base number is reduced; manually removing egg masses in the peak egg laying period; catching larvae by using a net bag in a larva incubation peak period; spraying Bacillus thuringiensis or fenitrothion emulsion in larval stage.
4. The method for rapidly recovering the potamogeton phophyllus population according to any one of claims 1 to 3, wherein the step S1 further comprises:
s1.6, spirogyra treatment: the duty is arranged and irrigated; when a small area occurs, manual salvage processing is adopted; spraying triphenyltin acetate WP when the occurrence area reaches 30%, and not draining water outwards 30 days after application.
5. The method for rapidly recovering an ophthalmia glabra population according to claim 1, wherein the S2 further comprises:
s2.4, not scattering soil blocks with a propagule bank; when seeds are thrown, the seeds need to be thrown on the water surface evenly.
6. The method for rapidly recovering an ophthalmia glabra population according to claim 1, wherein the S3 further comprises:
s3.4 preventing and treating hydroplania nubilalis: adult insects are killed by utilizing the trap lamp, so that the insect source base number is reduced; manually removing egg masses in the peak egg laying period; catching larvae by using a net bag in a larva incubation peak period; spraying Bacillus thuringiensis or fenitrothion emulsion in larval stage.
7. The method for rapidly recovering the potamogeton phophyllus population according to claim 1 or 6, wherein the step of S3 further comprises:
s3.5, spirogyra treatment: the duty is arranged and irrigated; when a small area occurs, manual salvage processing is adopted; spraying triphenyltin acetate WP when the occurrence area reaches 30%, and not draining water outwards 30 days after application.
8. The method for rapidly recovering an ophiocordyceps lucidus population according to claim 1, wherein the step of controlling the biological quantity of the ophiocordyceps lucidus population in S4 is to increase the release of grass carp or to manually harvest the grass carp.
CN202211455284.4A 2022-11-21 2022-11-21 Method for rapidly recovering ophthalmia glabra population Pending CN115812549A (en)

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