KR20040063260A - Artificial method of cultivation of Zostera marina L. - Google Patents

Abstract

PURPOSE: A method of artificially cultivating eelgrass(Zostera marina) comprising the steps of collecting seeds from natural eelgrass, preparing an artificial eelgrass seedling and planting the eelgrass seedling is provided. It permits restoration of a destructed eelgrass forest, thereby recovering marine ecosystems. CONSTITUTION: The eelgrass is cultivated by the following processes consisting of: harvesting seeds from natural eelgrass, germinating the eelgrass seeds; preparing an artificial seedling and planting the eelgrass seedling. In the eelgrass seeds germinating process, eelgrass seeds are soaked in seawater for 40 to 60 days, treated at 3 to 7deg.C and germinated at 4 to 6deg.C. The step for harvesting seeds from natural eelgrass contains collecting a spore and separating a small rachis and then culturing the separated small rachis.

Description

Artificial method of cultivation of Zostera marina L.

The present invention relates to a method of artificial cultivation of jaalpi.

It protects the seabed topography with strong winds and blue waves, supplies oxygen to the water through photosynthesis, cleans water pollution of coastal waters through metabolic activities, and is important as a spawning ground, habitat, and hideout where aquatic organisms lay eggs and grow. It is seaweed that plays a role. In addition, as a food source for fish and shellfish, it contributes greatly to the increase of fishery income by increasing fishery resources. Representing the temperate sea area, which inhabits the colonies in the inner sea, where the waves of the entire sea range from Baeknyeong Island on the west coast of Korea to Taean Peninsula, Byeonsan Peninsula, South Coast of Wando, Gwangyang Bay, Namhae, Busan Gadeok Island, Jeju Island and Ulju-gun, Gyeongbuk It is seaweed.

However, habitats are rapidly decreasing due to the influx of various pollutants due to industrialization and urbanization, construction of coastal industrial complexes, and civil engineering works such as reclamation, reclamation and dredging of the coast. Therefore, the demand for resource conservation and restoration is increasing, and researches on the ecology, pollen formation and early occurrence of zalpi in Chungmu area have been conducted in an effort to restore the destroyed marine ecosystem. However, research on artificial cultivation of Zalpi has not been conducted until now.

In order to solve the above problems, an object of the present invention is to provide a method for harvesting artificially cultivated seed of Jaalpi, and mass production by artificially cultivating the seed. In other words, by collecting seeds from wild zalpi, and developing artificial cultivation production technology to germinate, grow, and transplant by creating an artificial environment indoors, to restore the seaweed forest of the jaalpi.

1 is harvested jaalpied seeds.

2 is a seed in which cotyledons have occurred in one part of a seed;

Fig. 3 is a seedling in which the root and the foliar branched on the fifth day after sowing.

Figure 4 Seedling 20 days after sowing.

5 seedling 30 days after sowing.

6 is a seedling reaching a height of 86cm 6 months after sowing.

The artificial cultivation method of the present invention for the above object is characterized in that it comprises a step of harvesting the seed of the seedlings, germinating the seed of the seedlings, producing a seedling artificial seedling, and implanting the seedling artificial seedlings. .

One embodiment of the present invention is characterized in that the step of harvesting the seeds of the jaalpius comprises the step of separating the digestive axis by harvesting the gonads of the jaali and culturing the separated digestive axis.

One embodiment of the present invention is characterized in that the step of germinating the seed seeds including storing the seeds in natural sea water and low temperature treatment.

In one embodiment of the present invention, in the step of germinating seed seeds, it is characterized in that the germination at a temperature of 5 ℃ to 20 ℃.

In one embodiment of the present invention, the step of germinating the seed of the zalpi is stored for 40 days to 60 days in the natural sea water, and after low temperature treatment at 3 ℃ to 7 ℃, comprising germinating at 4 ℃ to 6 ℃ Characterized in that.

One embodiment of the present invention is characterized in that the step of producing artificial seedlings including a method of continuous watering natural seawater.

The present invention will be described in detail with reference to the following examples. However, the present invention is not limited to the following examples.

Example 1

Harvesting Seeds of Jalpi

In May ~ June 2001, the germline of Jalpi was collected from Hwayang-myeon, Yeosu-si, Jeonnam. Then, the digestive axis was placed in a circular tank (156cm in diameter, 61cm in height) in the incubation tube and matured for 1 month. At this time, natural sea water was used, the water temperature was room temperature (20 ℃ to 25 ℃), the sunshine time was to be 10 to 16 hours per day. Zalpi's digestive system initially floated on the surface of the seawater, but matured to dark brown, sinking to the bottom of the tank. Seeds were harvested by grinding down the sunk mature digestion axis. The harvested seeds of zalpi were 25% light green, 35% light brown and 40% dark brown. Seeds were about 1 mm in diameter and 3-4 mm in length, with a wet weight of about 11 mg. 1 is a photograph of harvested seeds.

Example 2

Germination of Seeds

Experiment 1

The seed of Zalpi was stored in natural seawater for 20 days, and then treated at low temperature by storing for 20 days in an environment of 5 ° C. and 2000 lux illuminance. Then, in each of the low-temperature incubators (EYELA, MTI-202) graded at 5 ° C, 10 ° C, 15 ° C, 20 ° C, and 25 ° C, put 1L beaker in 5 cm of soil and spray 50 seeds on it. After covering the soil 1cm high and filled with filtered seawater and sealed with transparent vinyl. Culture conditions were set to 3000 lux illuminance, light cycle 12L: 12D, and the exchange of the culture solution was carried out every 5 days.

Experiment 2

The germination experiment of the seed of Zalpi was carried out in the same manner as in Experiment 1 except that the natural seawater storage period was 40 days.

Experiment 3

The germination experiment of the seed of Zalpi was carried out in the same manner as in Experiment 1 except that the natural sea water storage period was 60 days.

The following table shows germination seed germination rates of the experiments 1 to 3.

Germination rate (%) = number of seeds germinated / total number of seeds × 100

Temperature (℃) 5 10 15 20 25 Experiment 1 31 9 6 2 - Experiment 2 67 29 14 5 - Experiment 3 88 63 32 14 6

As a result, the germination rate of seeds increased with the passage of the natural seawater storage period of the seeds, and the germination temperature showed the highest germination rate at 5 ℃, and decreased with increasing temperature and hardly germinated at 25 ℃. .

Example 3

Production of artificial seedlings of Zalpi

Zalpi seeds harvested by the method of Example 1 were stored in natural seawater for 60 days by the method described in Example 2, followed by low temperature treatment for 20 days. Then, 14 pieces of FRP round tanks (diameter 110cm, height 55cm) were put in soil 20cm high, sown at 3cm intervals, and covered with 1cm soil. The natural seawater was continuously watered with 45mm PVC pipe, the water temperature was room temperature (20 ℃ -25 ℃), and the sunshine time was 12-14 days a day. The culture was carried out from December 3, 2001 to June 2002.

2 is a view showing that the epidermis peeled from one part of the seed and cotyledon of about 300 μm in diameter was generated. After the cotyledon grows about 3 to 5 mm, the root and the foliar branch, which is a photograph of FIG. 3. Figure 4 is a branch branched from the cotyledon cotyledon 2 to 4 branches, the base of the foliar elongated into a compound, the upper part is a single leaf. Figure 5 is a complex branching of three or five when the leaflets of Zalpi growth more than 5cm. Seeds remained in the lobe base even when the leaves grew more than 20cm and disappeared as the roots developed. Zalpi's leaflets grew rapidly in February, reaching 86cm in June, with 5 leaflets, 4-6 roots of brown roots, 4 roots of white roots, and 4 roots of 9 roots. (FIG. 6).

The following table shows the growth of monthly seedling seedlings.

(Unit: cm) December January February In March In May June 4.5 ± 1.5 8.5 ± 2.2 14.5 ± 3.5 43.5 ± 4.6 62.5 ± 7.5 86 ± 9.6

Example 4

Transplantation of Xalpi Artificial Seedlings

The artificial seedlings of Zalpi produced by the method of Example 3 were transplanted at a distance of 10 m from the intertidal zone in the sea where the ecosystem was destroyed by the small fishing net. At low tide, the water depth was about 0.6 ~ 1.0 m, the low quality was fine mud, and the turbidity was very high due to the blue. Artificial seedlings of Zalpi were separated into two 30cm intervals, and planted at a depth of about 5cm.

After 2 months of transplantation, it survived more than 95%, and grew to 98 ± 15cm in size and 0.81 ~ 2cm in width.

The present invention enables the harvesting of artificial seedlings by harvesting seeds of artificial seedlings and providing a method of cultivating them indoors. This will restore the destroyed zalpi forests to achieve restoration of marine ecosystems.

Claims (6)

In the artificial cultivation method of jalpi, Harvesting the seedlings; Germinating seed seeds; Producing artificial seedlings of the jaal; A method of artificial cultivation of jaalpi, characterized in that it comprises the step of implanting the artificial seedling of jaalpi. The method of claim 1, wherein the step of harvesting the seed of the skin of the artificial cultivation method of the artificial skin of Jaalpi, characterized in that the step of taking the gonads of the skin to separate the digestive axis and culturing the separated digestive axis. The method of claim 1, wherein germinating the seed of the seedlings comprises storing the seed of the seedlings in natural sea water and cold-treating. The method of claim 1 wherein the step of germinating the seed seed germination comprises artificial germination at a temperature of 5 ℃ to 20 ℃. The method of claim 1, wherein the germinating germination of the seed comprises storing the seed in the natural sea water for 40 days to 60 days, low temperature treatment at 3 ° C to 7 ° C, and then germination at 4 ° C to 6 ° C. Jalpy artificial cultivation method, characterized in that. The method of claim 1, wherein the step of producing artificial seedlings, including the method of continuous watering natural seawater.