CN104126374A - Wild harvesting method of Zostera marina L. seeds - Google Patents

Wild harvesting method of Zostera marina L. seeds Download PDF

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Publication number
CN104126374A
CN104126374A CN201310431190.8A CN201310431190A CN104126374A CN 104126374 A CN104126374 A CN 104126374A CN 201310431190 A CN201310431190 A CN 201310431190A CN 104126374 A CN104126374 A CN 104126374A
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China
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spray
string bag
seed
string
seeds
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CN201310431190.8A
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CN104126374B (en
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郑凤英
李乐乐
刘雪芹
陈玉
金艳梅
张伟
韩晓弟
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Shandong University Weihai
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Shandong University Weihai
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Abstract

The invention relates to a wild harvesting method of Zostera marina L. seeds. The method comprises the following steps: at a low-tide stage when peels on first spathe inflorescences of most flower lateral branches are initially cracked, harvesting flowering branches, putting the harvested flowering branches into 20-mesh bolting silk string bags till each string bag is filled by a half, tightening the mouths of the string bags, connecting the plurality of string bags together, and locally putting the connected string bags back into sea water; 15 days later, taking the string bags out and cleaning substances attached to the surfaces of the string bags; about 30 days later, taking the string bags out, locally pouring the flowering branches in the string bags into a plastic water bucket, adding sea water, stirring vigorously, and picking out residual flowering branches on an upper layer after seeds fall to the bottom of the bucket; adding sea water repeatedly, stirring, and pouring out immature seeds floating on the upper layer; and filtering large shellfishes out by using a 10-mesh mesh sieve in sea water, filtering small shellfishes and residues out by using a 30-mesh mesh sieve, and selecting plump Zostera marina L. seeds. By using the wild harvesting method, a high seed harvesting rate is ensured under the condition of low cost, and damages to populations caused by harvesting of the flowering branches are reduced to the maximum extent. The wild harvesting method can be applied to any Zostera marina L. growing sea area.

Description

A kind of field harvesting method of grass wrack seeds
Technical field
The results field of seaweed seeds under the present invention, relates to a kind of field harvesting method of grass wrack seeds.
Background technology
Zostera marina ( zostera marinal.) belong to ocean spermatophyte, be once widely distributed in China Shandong, Hebei and Liaoning Coast, but recent two decades comes China Zostera marina population nearly 80% to degenerate, and therefore in China, has started in recent years the upsurge of its population and ecosystem restoration thereof.Because a large amount of adult transplanting can bring larger destruction to this rare Zostera marina provenance, so adopt results seed and be seeded into this method of reparation marine site, more and more favored.Therefore, effective results of grass wrack seeds are by the element task that is this restorative procedure.
The Zostera marina in Shandong, Hebei and marine site, Liaoning generally in 7, enter seed maturity August.Long 70~the 110cm of its ripe spray, upright, there are 3~6 branches; Each branch can produce a plurality of spathe inflorescences, but only has the seed in 2~3 inflorescences of tip just likely ripe before spray death, has results and is worth.Zostera marina mature seed ovalize or avette, light brown, long 2.5~4.0mm, wide 1.5~2.0mm.
Grass wrack seeds volume is little, than great, plant skin cracking after ripe, seed shedding, just falls into seabed very soon, makes the collection results of grass wrack seeds have certain difficulty.For preventing that mature seed from falling into seabed and being difficult to collect, the U.S. once reported and adopted the round tank with end leakage device, in bucket, seawater was housed, and will gather in next Zostera marina spray running into tank seawater, and it is inflated temporarily and is supported, the seed in batches maturation being come off is collected.This device needs special culture apparatus, power consumption greatly, economical and practical not.Domestic once announced " a kind of apparatus for collecting sea-grass seeds " invented, and American process had to larger improvement, but still need specific skinny device, making method relative complex.
Summary of the invention
The invention provides a kind of low cost, simple to operate and effective grass wrack seeds harvesting method, can be applicable in any Zostera marina growth marine site.It is characterized in that comprising the following steps:
(1) determining of seed harvest time: because Zostera marina is bloomed, result is closely related with temperature, so different waters grass wrack seeds harvest time difference slightly.When much research shows grass wrack seeds maturation, ocean temperature is more than 20 ℃, therefore when reaching 20 ℃, the water temperature in results marine site can carry out weekly tracing observation, treat in marine site that the pericarp on the 1st spathe inflorescence on most of spray side shoot just ftractures, spray basal part of stem starts chlorosis, can start results;
(2) spray is collected: in the time of low water, spray is gathered, because spray basal part of stem starts to decay, so be manually easy to whole strain spray to pull up, the spray of adopting is packed in the 20 object bolting silk string bags immediately;
(3) seed shedding: the string bag is filled to 1/2 completely, first string bag mouth is tightened, more a plurality of string bags are cascaded by plastic ties, plastic ties two ends are tied with weight and put back in seawater on the spot, so that mature seed comes off in the string bag;
(4) string bag cleaning: after approximately 15 days, the string bag is taken out to the attachment of cleaning outside when spring low water, unimpeded to keep string bag maritime interior waters to flow;
(5) the residual branch cleaning of spray: after approximately 30 days, the string bag is taken out again, now mature seed all comes off, on the spot all sprays in a string bag are poured in a plastic bucket, add seawater, after firmly stirring, at the bottom for the treatment of in bucket that seed all falls into barrel, the residual branch of the spray on bucket upper strata is pulled out, many experiments shows that the string bag is filled to 1/2 when full, after 30 days, seed all comes off, although spray in the string bag can part be rotted, due to seawater in the string bag, flow unimpeded, mature seed, also imputrescibility of immature seed even;
(6) mature seed primary election: the seed at the bottom of now falling into barrel has many maturity gastrointestinal disease patients, embryotic seed is white in color, light specific gravity, in bucket, add on the spot seawater, after stirring, they float over upper strata, and when seawater is poured out, these immature seed major parts all flow out with seawater, only mature seed and some little shellfishes etc. stayed;
(7) mature seed is selected: in seawater, with 10 object mesh screens, first larger shellfish is leached on the spot, then screen out with shellfish and residue that 30 object mesh screens are less than grass wrack seeds by diameter, finally select the full Zostera marina mature seed of form.
The technology of the present invention effect is: collect the string bag and manufacture simply, cost is low; The 20 object string bags are filled to 1/2 completely, can not affect seawater and circulate and exchange, although the spray in the string bag can be partly rotten, because seawater is mobile unimpeded in the string bag, seed (even if immature seed) is imputrescibility also; Gather the grasp of spray time good, because spray basal part of stem starts to decay, thus be manually easy to whole strain spray to pull up, and do not injure other clone plant that is connected growth with spray; Make full use of Zostera marina growth marine site briny environment, can effectively guarantee to have continued maturation in the string bag one month of seed on spray.Therefore, the present invention, under the conditions such as the light of natural waters, temperature, utilizes the simple and easy string bag to bring temporarily leaf water bloom branch up, under low-cost, low labour intensity condition, guarantee the utilization percent that grass wrack seeds is high, and reduced to greatest extent to gather the destruction of spray to Zostera marina population.
Accompanying drawing explanation
1, Fig. 1 pulls the residual branch of spray out photo
2, Fig. 2 removes the grass wrack seeds photo after the residual branch of spray
Embodiment
A kind of low cost of the present invention, simple to operate and effective grass wrack seeds harvesting method, can be applicable in any Zostera marina growth marine site.Comprise following examples, the following examples can further illustrate the present invention, but do not limit the present invention in any way.
embodiment 1:twin islet gulf, Weihai in Shandong province city grass wrack seeds harvesting method, concrete steps are:
(1) determining of seed harvest time: in marine site, twin islet gulf, Weihai in Shandong province July from the beginning of week about to spray being carried out to maturity observation in Zostera marina growth marine site, when the pericarp on the 1st spathe inflorescence on most of spray side shoot ftractures, now corresponding spray basal part of stem starts chlorosis, rots, and can start results;
(2) spray is collected: in the time of low water, spray is gathered, because spray basal part of stem starts to decay, so be manually easy to whole strain spray to pull up, the spray of adopting is packed in the bolting silk string bag immediately.String bag nylon line suture, stitch finer and closely woven (5mm), during stitching by yarn edge contraction twice, in being sewn to, in case suture is damaged, mesh 20 orders, base diameter 15cm, long 90cm, prop up with the steel wire of diameter 5mm bottom;
(3) seed shedding: the string bag is filled to 1/2 completely, string bag mouth is first tightened with rubber band, then 7~8 string bags are cascaded by plastic ties, plastic ties two ends are tied with to be put back in seawater compared with ratchel on the spot, allow mature seed self falling enter in the string bag, and on the water surface, use buoy mark;
(4) string bag cleaning: the string bag is taken out to the attachment of cleaning outside after approximately 15 days when spring low water, the string bag is entered back in former marine site more subsequently;
(5) the residual branch cleaning of spray: after approximately 30 days, the string bag is taken out again, now mature seed all comes off, on the spot all sprays in a string bag are poured in a plastic bucket, add seawater, after firmly stirring, at the bottom for the treatment of in bucket that seed all falls into barrel, the residual branch of the spray on bucket upper strata is pulled out, so repeatedly, the complete liquidation of the residual branch of spray is fallen;
(6) mature seed primary election: add on the spot seawater in bucket, after stirring, the prematurity white seed that floats over upper strata is poured out with seawater, so repeatedly, immature seed is almost all removed;
(7) mature seed is selected: in seawater, with 10 object mesh screens, first larger shellfish is leached on the spot, then screen out with shellfish and residue that 30 object mesh screens are less than Zostera marina by diameter, finally select the full Zostera marina mature seed of form.
embodiment 2:dalian From Zhangzi Islands China grass wrack seeds harvesting method, concrete steps are:
(1) determining of seed harvest time: week about maturity observation is carried out to spray in the marine site of growing at Zostera marina at Zhang Zidao Island, China in mid-August, when the pericarp on the 1st spathe inflorescence on most of spray side shoot ftractures, now corresponding spray basal part of stem starts chlorosis, rots, and can start results;
(2) spray is collected: in the time of low water, spray is gathered, because spray basal part of stem starts to decay, so be manually easy to whole strain spray to pull up, the spray of adopting is packed in the bolting silk string bag immediately.String bag nylon line suture, stitch finer and closely woven (5mm), during stitching, by yarn edge contraction twice, in being sewn to, in case suture is damaged, mesh is 20 orders, base diameter 15cm, long 90cm, prop up with the steel wire of diameter 5mm bottom;
(3) seed shedding: the string bag is filled to 1/2 completely, string bag mouth is first tightened with rubber band, then 7~8 string bags are cascaded by plastic ties, plastic ties two ends are tied with to be put back in seawater compared with ratchel on the spot, allow mature seed self falling enter in the string bag, and on the water surface, use buoy mark;
(4) string bag cleaning: when spring low water, the string bag is taken out to the attachment of clearing up outside it after approximately 15 days, the string bag is entered back in former marine site more subsequently;
(5) the residual branch cleaning of spray: after approximately 30 days, the string bag is taken out again, now mature seed all comes off, on the spot all sprays in a string bag are poured in a plastic bucket, add seawater, after firmly stirring, at the bottom for the treatment of in bucket that seed all falls into barrel, the residual branch of the spray on bucket upper strata is pulled out, so repeatedly, the complete liquidation of the residual branch of spray is fallen;
(6) mature seed primary election: add on the spot seawater in bucket, after stirring, the prematurity white seed that floats over upper strata is poured out with seawater, so repeatedly, immature seed is almost all removed;
(7) mature seed is selected: in seawater, with 10 object mesh screens, first larger shellfish is leached on the spot, then screen out with shellfish and residue that 30 object mesh screens are less than Zostera marina by diameter, finally select the full Zostera marina mature seed of form.

Claims (1)

1. a field harvesting method for grass wrack seeds, is characterized in that concrete steps are as follows:
(1) determining of seed harvest time: can carry out weekly tracing observation when the water temperature in results marine site reaches 20 ℃, treat in marine site that the pericarp on the 1st spathe inflorescence on most of spray side shoot just ftractures, spray basal part of stem starts chlorosis, can start results;
(2) spray is collected: in the time of low water, spray is gathered, manually the spray of adopting is packed in the 20 object bolting silk string bags immediately;
(3) seed shedding: the string bag is filled to 1/2 completely, first string bag mouth is tightened, more a plurality of string bags are cascaded by plastic ties, plastic ties two ends are tied with weight and put back in seawater on the spot, so that mature seed comes off in the string bag;
(4) string bag cleaning: after approximately 15 days, when the time of low water, the string bag is taken out to its outer attachment of cleaning, unimpeded to keep string bag maritime interior waters to flow;
(5) the residual branch cleaning of spray: after approximately 30 days, the string bag is taken out again, now mature seed all comes off, on the spot all sprays in a string bag is poured in a plastic bucket, add seawater, after firmly stirring, at the bottom for the treatment of in bucket that seed all falls into barrel, the residual branch of the spray on bucket upper strata is pulled out;
(6) mature seed primary election: continue to add on the spot seawater in bucket, after stirring, the prematurity white seed that floats over upper strata is poured out, only mature seed and some shellfish larvaes are stayed;
(7) mature seed is selected: in seawater, with 10 object mesh screens, first larger shellfish is leached on the spot, then screen out with shellfish and residue that 30 object mesh screens are less than grass wrack seeds by diameter, finally select the full Zostera marina mature seed of form.
CN201310431190.8A 2013-09-22 2013-09-22 A kind of field harvesting method of grass wrack seeds Expired - Fee Related CN104126374B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105557498A (en) * 2015-12-28 2016-05-11 山东大学(威海) Ecological restoration method for zostera marina sea grass bed
CN106068763A (en) * 2016-06-21 2016-11-09 中国科学院昆明植物研究所 The method for cleaning of pittosporum tobira seed
CN110679464A (en) * 2018-11-29 2020-01-14 山东大学 Method for effectively harvesting seaweed fruits of red fiber shrimps in field

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CN201533528U (en) * 2009-11-13 2010-07-28 山东东方海洋科技股份有限公司 Apparatus for collecting sea-grass seeds
CN102405825A (en) * 2011-07-15 2012-04-11 中国科学院海洋研究所 Ecological recovering method of sublittoral zone Zosteraceae sea grass bed
CN103081651A (en) * 2011-11-02 2013-05-08 中国海洋大学 Method for collecting zostera marina seeds

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KR20040063260A (en) * 2003-01-06 2004-07-14 대한민국(관리부서:국립수산과학원) Artificial method of cultivation of Zostera marina L.
CN201533528U (en) * 2009-11-13 2010-07-28 山东东方海洋科技股份有限公司 Apparatus for collecting sea-grass seeds
CN102405825A (en) * 2011-07-15 2012-04-11 中国科学院海洋研究所 Ecological recovering method of sublittoral zone Zosteraceae sea grass bed
CN103081651A (en) * 2011-11-02 2013-05-08 中国海洋大学 Method for collecting zostera marina seeds

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潘金华 江鑫等: "大叶藻种子的收集和保存技术研究", 《中国藻类学会第八次会员大会暨第十六次学术讨论会论文摘要集》, 11 November 2011 (2011-11-11) *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105557498A (en) * 2015-12-28 2016-05-11 山东大学(威海) Ecological restoration method for zostera marina sea grass bed
CN106068763A (en) * 2016-06-21 2016-11-09 中国科学院昆明植物研究所 The method for cleaning of pittosporum tobira seed
CN106068763B (en) * 2016-06-21 2018-12-14 中国科学院昆明植物研究所 The method for cleaning of pittosporum tobira seed
CN110679464A (en) * 2018-11-29 2020-01-14 山东大学 Method for effectively harvesting seaweed fruits of red fiber shrimps in field
CN110679464B (en) * 2018-11-29 2021-05-04 山东大学 Method for effectively harvesting seaweed fruits of red fiber shrimps in field

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