CN115804299A

CN115804299A - Method for grafting sugarcane by using pasture and application

Info

Publication number: CN115804299A
Application number: CN202211629617.0A
Authority: CN
Inventors: 朱建民
Original assignee: Hainan Zhaode Agricultural Technology Co ltd
Current assignee: Hainan Zhaode Agricultural Technology Co ltd
Priority date: 2021-01-28
Filing date: 2021-01-28
Publication date: 2023-03-17
Also published as: CN112997709A

Abstract

The invention provides a method for grafting sugarcane by pasture and application, and relates to the technical field of agricultural planting, wherein the method for grafting sugarcane by pasture comprises the following steps: s1: sawing a section of sugarcane for sampling, removing knots, and sawing an opening of the sugarcane to obtain a grafting interface; s2: disinfecting the grafting interface by using a disinfectant, cutting the pasture seedlings, cutting the lower ends of the pasture seedlings into 45-degree fractures, cutting the front and back surfaces of the fractures along the length direction of the pasture seedlings to form inserting parts, wherein the length of each inserting part is equal to the depth of the grafting interface; s3: inserting the inserting part into the grafting interface to enable the inserting part to be tightly attached to the sugarcane peel and tightly pressed by hands; s4: firstly, tightly binding the grafting connector by using a plastic film, and then binding the sugarcane and the forage grass seedlings; s5: the insect-proof medicine is scattered on the connecting part of the insertion part and the grafting interface. The grafted forage grass contains sugarcane growth genes, has the capability of sugarcane production and growth, and can grow tall and large leaf size, and the nutrients are improved compared with the non-grafted forage grass.

Description

Method for grafting sugarcane by using pasture and application

The application is a divisional application with the application date of 2021, 28.01 months and the application number of 202110115705.8 and the name of the invention, namely a method for grafting the pasture to the sugarcane.

Technical Field

The invention relates to the technical field of agricultural planting, in particular to a method for grafting pasture to sugarcane and application of the method.

Background

The pasture is a common name of pasture plants, commonly called as burclover, is a perennial flowering plant, the most notable of which is the purple pasture used as the pasture, the pasture is various and is mostly a wild herbaceous plant, the pasture is a perennial herbaceous plant, is similar to clover, is drought-resistant, cold-resistant and heat-resistant, has high yield and high quality, and can improve soil, so that the pasture is widely cultivated and mainly used for preparing hay, silage or used as the pasture. The existing pasture can be used for feeding livestock, such as cattle, sheep and the like, but the nutrient content of the pasture is low, and the nutrition of the livestock cannot meet the basic requirement easily, which is a problem to be solved urgently at present.

Disclosure of Invention

Based on the above, it is necessary to provide a method for grafting the pasture to the sugarcane and an application of the pasture grafted sugarcane in improving the anti-drowning capability of the pasture or improving the nutrient content of the pasture, aiming at the problem that the nutrient content of the existing pasture is low.

The invention provides application of pasture grafted sugarcane in improving the drowning resistance of pasture or improving the nutritional ingredients of pasture.

Preferably, the grafting method for grafting the pasture onto the sugarcane comprises the steps of cutting the grafting interface of the sugarcane, cutting the pasture seedling to form a splicing part, inserting the pasture splicing part into the grafting interface of the sugarcane, binding the sugarcane and the pasture seedling, scattering insect-proof medicine and transplanting to obtain the grafted pasture.

Preferably, the transplanting is performed by using a special fertilizer, the special fertilizer comprises an organic fertilizer, and the organic fertilizer comprises the following raw materials in parts by weight: 17 to 22 portions of peanut cake, 14 to 17 portions of biogas residue, 10 to 20 portions of fruit residue, 6 to 12 portions of cow and sheep manure, 17 to 26 portions of edible fungus residue, 6 to 9 portions of shell powder and 10 to 20 portions of bagasse.

The invention provides a method for grafting sugarcane by pasture, which comprises the following steps:

s1: cutting open a grafting port, sawing a section of sugarcane by using a cutter for sampling, removing knots in the sugarcane, and cutting an opening of the sugarcane saw by using the cutter to obtain the grafting port;

s2: disinfecting the grafting interface by using a disinfectant, cutting grass seedlings by using a cutter, cutting the lower ends of the grass seedlings into 45-degree fractures, cutting the grass seedlings along the length direction of the grass seedlings on the front back surfaces of the fractures to form inserting parts, wherein the cross section of each inserting part is consistent with the shape of the grafting interface, the length of each inserting part is equal to the depth of the grafting interface, and the inserting parts and the grafting interfaces are both smooth;

s3: inserting the inserting part into the grafting interface to enable the inserting part to be tightly attached to the sugarcane peel and tightly pressed by hands;

s4: the grafting connector is firstly tied by using a plastic film special for grafting, and then sugarcane and forage grass seedlings are bound;

s5: and (3) spraying insect-proof medicines on the connecting part of the inserting part and the grafting interface so as to prevent insects and ants from biting through the film.

The method for grafting the pasture with the sugarcane can graft the pasture with the sugarcane, so that the later-growing pasture contains the genes for growing the sugarcane and the genes of the pasture, the pasture has the capability of growing the sugarcane, and has the advantages of high growth, large leaf size and much improved nutrients compared with the non-grafted pasture. The nutrient content is increased, the sugar content is also increased, the forage grass is used for feeding livestock, and the edible taste and appetite of the livestock are improved. In addition, the pasture absorbs the genes of the sugarcane, increases the nutrient absorption capacity, improves the anti-drowning capacity, and has good effects on drought resistance and waterlogging resistance.

In one embodiment, the sample sugarcane in S1 is 15cm-20cm in length.

In one embodiment, the grafting port of the sample sugarcane in the S1 is square, the side length of the grafting port is 1cm-1.5cm, and the depth of the grafting port is 2cm-3cm.

In one embodiment, the length of the grass seedling sample in S2 is 5cm-6cm, and 1-2 full axillary buds grow on the top of the grass seedling sample, and the full axillary buds are wrapped by a single-layer film.

In one embodiment, the middle part of the front surface and the back surface of the fracture in the S2 is cut along the length direction of the grass seedlings.

In one embodiment, the anti-insect agent in S5 is a clear insecticide powder, the clear insecticide powder is sprayed by using a spray gun, and the connection part is sprayed once every 10 to 15 hours.

In one embodiment, the grafted grass seedlings and the sugarcane are transplanted into an arched shed, the temperature of the arched shed in the day and at night is controlled to be 25-27 ℃ and 20-22 ℃, the humidity is controlled to be 70-90%, and the top of the arched shed is shielded from light and the two sides of the arched shed are exposed to light.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below. It is understood that the specific details described below are merely exemplary of some embodiments of the invention, and that the invention may be practiced in many other embodiments than as described herein. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without any inventive step, are within the scope of the present invention.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only and do not represent the only embodiments.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

The invention provides a method for grafting pasture on sugarcane, which can graft pasture and sugarcane, so that the grafted pasture has genes for the growth of the sugarcane, and the original genes of the pasture cannot be damaged. The pasture has the two genes at the same time, so that the pasture has the capability of producing and growing the sugarcane, the growing height of the pasture is higher than that of the common pasture, the size of the pasture blade is larger than that of the common pasture, and the nutrients of the pasture are greatly improved compared with those of the non-grafted pasture. The forage grass improves the nutrient content and the sugar content, and the forage grass can improve the eating taste and the appetite of the livestock when the livestock is fed by the forage grass, so that the livestock can obtain more nutrition and grow fast. In addition, the pasture absorbs the genes of the sugarcane, and the capacity of the sugarcane for absorbing soil nutrients is increased, so that the anti-drowning capacity of the pasture is improved, the effects of drought and waterlogging resistance are good, and the growth adaptability of the pasture is stronger.

The method for grafting the sugarcane by the pasture comprises the following steps:

s1: cutting the grafting interface, sawing a section of sugarcane by using a cutter to sample, removing knots in the sugarcane, and cutting an opening in the sugarcane by using the cutter to obtain the grafting interface. The lush growing and body-shape sugarcane can be selected, so that the pasture can obtain the sugarcane gene with higher quality. The selected sugarcane is opened at the place with smooth surface and no damaged cortex.

S2: the pasture grass seedling is cut by the cutter, the lower end of the pasture grass seedling is cut into a 45-degree fracture, and the front surface and the back surface of the fracture are cut along the length direction of the pasture grass seedling to form an inserting part, so that the contact area of the pasture grass and the sugarcane is increased sufficiently. The cross section shape of grafting portion is unanimous with the shape of grafting interface, and the forage grass of being convenient for can insert stably in the grafting interface, and can keep fixed. The length of grafting portion equals the degree of depth of grafting interface, and grafting portion is the level and smooth form with the grafting interface, is convenient for make whole grafting portion can insert the grafting interface completely in, fully contacts with the sugarcane, makes more sugarcane genes can graft to pasturing grass seedling on. In addition, before moving the blades, disinfectant needs to be sprayed on the surfaces of the sugarcanes. The types of the disinfectant solution are various, and the disinfectant solution of the embodiment can be prepared by mixing iron color arrow and lysimachia sikokiana in the same mass part, adding water with the mass 1.5-2 times of that of the mixture, decocting for 50-60 min, filtering, mixing the filtrate with hydrogen peroxide with the concentration of 2-4% in a mass ratio of 1: 0.3-0.4, and uniformly stirring.

S3: and inserting the inserting part into the grafting interface to enable the inserting part to be tightly attached to the sugarcane peel and tightly pressed by hands. The grafting interface is in an inclined state, the inserting part is obliquely inserted into the sugarcane, the outer end of the inserting part is tightly attached to the sugarcane peel, and the force for inserting the inserting part into the grafting interface is applied to the inserting part by hand, so that the inserting part is tightly inserted into the grafting interface.

S4: the grafting connector is firstly tied by using a plastic film special for grafting, and then the sugarcane and the forage grass seedlings are tied. The plastic film can be a common preservative film which is soft and is not easy to damage grass seedlings. Specifically, the preservative film is wound for tens of circles at the connecting part of the grafting connector and the pasture surface, and the part of the pasture seedling, which is not inserted into the sugarcane, is also wound with the preservative film and is bound with the sugarcane.

S5: the connection part of the insertion part and the grafting interface is sprinkled with the insect-proof medicine to prevent insects and ants from biting through the film, and further prevent insects and ants from biting the insertion part and the grafting interface of the grass seedling.

S6: and (3) transplanting, namely, after the grafted forage grass seedlings are laid aside for 1-2 days, transplanting the forage grass seedlings to soil, watering the forage grass seedlings, applying special fertilizer, spraying nutrient solution, and performing daily management. The special fertilizer can be composed of an organic fertilizer and an inorganic fertilizer, wherein the inorganic fertilizer can be prepared by mixing 40-50 parts of nitrogen-phosphorus-potassium compound fertilizer, 1-3 parts of zinc sulfate, 2-3 parts of manganese sulfate, 1-2 parts of ferrous sulfate and 1-3 parts of gypsum, adding 0.8-1.2 times of water of the mixture, heating to dissolve the mixture, cooling, dehydrating and drying.

The organic fertilizer can be prepared by mixing 17-22 parts of peanut cakes, 14-17 parts of biogas residues, 10-20 parts of fruit residues, 6-12 parts of cow and sheep manure, 17-26 parts of edible fungus residues, 6-9 parts of shell powder and 10-20 parts of bagasse, feeding the mixture into a fermentation chamber, fermenting for 3-4 days, and fishing out and drying the mixture.

Mixing the prepared organic fertilizer and inorganic fertilizer, crushing, sieving and preparing into particles with the particle size of 0.17-0.3 mm by a machine.

In one embodiment, the sample sugarcane in S1 is 15cm-20cm in length, longer sugarcane can be convenient for inserting pasture seedlings, and stored nutrients are more. The grafting interface of the sample sugarcane is square, the side length of the grafting interface is 1cm-1.5cm, and the depth is 2cm-3cm. The specific side length can be determined according to the size of the inserting part of the grass seedling to be grafted.

In one embodiment, the length of the grass seedling sample in S2 is 5cm-6cm, 1-2 full axillary buds grow on the top of the grass seedling sample, and the full axillary buds are wrapped by a single-layer film.

In one embodiment, the pesticide in S5 is a clear insecticidal powder, and the clear insecticidal powder is sprayed by using a spray gun, and the joint part is sprayed once every 10 to 15 hours.

In one embodiment, the grafted grass seedlings and the sugarcane are transplanted into an arched shed, the temperature of the arched shed in the daytime and the temperature of the arched shed in the nighttime are controlled to be 25-27 ℃ and 20-22 ℃, the humidity is controlled to be 70-90%, and the top of the arched shed is shielded from light and the two sides of the arched shed are exposed to light.

The pasture cultivated by the method of the invention is compared with the non-grafted pasture on the anti-drowning effect, so that the excellent characteristics of the grafted pasture of the invention are highlighted conveniently:

after 100 pasture grass seedlings are grafted, synchronously planting and cultivating the grafted pasture grass seedlings and 100 non-grafted pasture grass seedlings, after all the pasture grass seedlings grow into pasture grass, placing all the pasture grass in deeper water, keeping the distance between the water surface and the top of the pasture grass at about 5cm, observing and recording the pasture grass every 2 hours, and counting the number of the survival pasture grass. The following data were obtained by experiment and are shown in table 1:

TABLE 1 survival rate of grafted and ungrafted forage grasses at different flooding times

Flooding time (h)	Survival rate of grafted forage grass	Survival rate of non-grafted forage grass
			0	100％	100％
2	99％	80％
			4	96％	65％
6	93％	60％
			8	93％	45％
10	90％	42％
			12	85％	30％
14	82％	15％
			16	80％	1％

Therefore, compared with the non-grafted pasture, the pasture cultivated by the grafting method provided by the invention is obviously improved in anti-drowning capability. Through observation and statistics for 16 hours, basically all the non-grafted pasture is dead, and the grafted pasture has the anti-drowning gene of sugarcane, so that the anti-drowning capability is obviously enhanced.

The forage grass cultivated by the method of the invention and the non-grafted forage grass are compared and analyzed on the feeding livestock, for example, sheep, and the nutrition effect of the grafted forage grass of the invention is highlighted by measuring the weight of the sheep at different time periods.

In order to avoid coincidence, 100 sheep were prepared, 50 sheep were fed with the grass grafted with sugarcane, and the other 50 sheep were fed with the non-grafted grass, the weight of the grass fed to each sheep was consistent each time, and other factors were controlled to be constant, each sheep was weighed every 5 days, and the average weight of 50 sheep fed with the grafted grass and the average weight of 50 sheep fed with the non-grafted grass were calculated, and the statistical results are as follows:

TABLE 2 average body weight of sheep fed grafted forage and undigested forage

Therefore, the weight of the livestock eating the grafted forage grass is increased more quickly than that of the livestock eating the common forage grass, and the grafted forage grass has high nutrient content, so that the livestock can obtain higher nutrition.

All possible combinations of the technical features in the above embodiments may not be described for the sake of brevity, but should be considered as being within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.

The above examples only show several embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the invention. It should be noted that various changes, substitutions and alterations can be made by those skilled in the art without departing from the spirit of the invention, and these are all intended to be covered by the scope of the invention. Therefore, the protection scope of the present invention should be subject to the claims.

Claims

1. An application of the herbage grafted sugarcane in improving the anti-drowning ability of herbage or improving the nutrient components of herbage.

2. The application of claim 1, wherein the method for grafting the pasture onto the sugarcane comprises the steps of cutting the grafting interface of the sugarcane, cutting the pasture seedling to form a splicing part, inserting the pasture splicing part into the grafting interface of the sugarcane, binding the sugarcane and the pasture seedling, spreading an insect-preventing medicine, and transplanting to obtain the grafted pasture.

3. The application of claim 2, wherein the transplanting is performed by using a special fertilizer, the special fertilizer comprises an organic fertilizer, and the organic fertilizer comprises the following raw materials in parts by weight: 17 to 22 parts of peanut cake, 14 to 17 parts of biogas residue, 10 to 20 parts of fruit residue, 6 to 12 parts of cow and sheep manure, 17 to 26 parts of edible fungus residue, 6 to 9 parts of shell powder and 10 to 20 parts of bagasse.

4. A method for grafting sugarcane by pasture is characterized by comprising the following steps:

s1: cutting a grafting interface, sawing a section of sugarcane by using a cutter to sample, removing knots in the sugarcane, and cutting an opening of the sugarcane saw by using the cutter to obtain the grafting interface;

s2: disinfecting the grafting connector by using a disinfectant, cutting grass seedlings by using a cutter, cutting the lower ends of the grass seedlings into 45-degree fractures, cutting the front and back surfaces of the fractures along the length direction of the grass seedlings to form inserting parts, wherein the cross section of each inserting part is consistent with the shape of the grafting connector, the length of each inserting part is equal to the depth of the grafting connector, and the inserting parts and the grafting connectors are smooth;

s4: the grafting connector is firstly tied by using a plastic film special for grafting, and then the sugarcane and the forage grass seedlings are tied;

5. The pasture grafting method for sugarcane according to claim 4, wherein the length of the sample sugarcane in S1 is 15cm-20cm.

6. The pasture grafting method for sugarcane according to claim 4, wherein a grafting port of the sample sugarcane in S1 is square, the side length of the grafting port is 1cm-1.5cm, and the depth of the grafting port is 2cm-3cm.

7. The method for grafting the sugarcane by the pasture grass as claimed in claim 4, wherein the length of the pasture grass seedling sample in S2 is 5cm-6cm, 1-2 full axillary buds grow on the top of the pasture grass seedling sample, and the full axillary buds are wrapped by a single-layer film.

8. The method for grafting the pasture grass onto the sugarcane according to claim 4, wherein the middle part of the front surface and the back surface of the fracture in the S2 is cut along the length direction of the pasture grass seedlings.

9. The pasture grafting method for the sugarcane according to claim 4, wherein the insect-resistant drug in S5 is a clear insecticidal powder, the clear insecticidal powder is sprayed by using a spray gun, and the connection part is sprayed once every 10-15 hours.

10. The method for grafting the pasture grass to the sugarcane, as claimed in claim 4, wherein the grafted pasture grass seedlings and the sugarcane are transplanted in an arched shed, the temperature of the arched shed in the day and at night is controlled to be 25-27 ℃ and 20-22 ℃ respectively, the humidity is controlled to be 70-90%, and the top of the arched shed is shaded and both sides of the arched shed are exposed to light.