CN111995448B

CN111995448B - Organic fertilizer for producing potato breeder's seeds

Info

Publication number: CN111995448B
Application number: CN202010950787.3A
Authority: CN
Inventors: 马玉龙; 马磊; 顾占杰; 马正龙; 黄勇; 何娟
Original assignee: Guyuan Tianqi Potato Industry Co ltd
Current assignee: Guyuan Tianqi Potato Industry Co ltd
Priority date: 2017-09-30
Filing date: 2017-09-30
Publication date: 2022-04-01
Anticipated expiration: 2037-09-30
Also published as: CN111995448A; CN107827491A; CN107827491B

Abstract

The invention belongs to the technical field of potato breeding, and particularly relates to an organic fertilizer with an insecticidal effect for producing potato breeder seeds. The organic fertilizer is prepared from the following raw materials: 5-10 parts of sophora alopecuroide, 7-10 parts of selenium-containing fructus trichosanthis stem, 10-15 parts of waste selenium-containing fructus trichosanthis, 10-15 parts of medlar branches and leaves, 10-15 parts of purslane, 3-5 parts of broom cypress, 10-15 parts of oleaster processing waste, 5-10 parts of waste vinasse, 5-7 parts of plant ash, 10-20 parts of manure, 5-10 parts of a solid culture medium and 1-2 parts of a fermentation microbial inoculum; can effectively reduce the dosage of the pesticide by 25 to 35 percent.

Description

Organic fertilizer for producing potato breeder's seeds

The application is a divisional application of the following patent applications, the application numbers of the original applications: 2017109195121, filing date: 2017-09-30, invention name: an organic fertilizer with insecticidal effect.

Technical Field

The invention belongs to the technical field of potato breeding, and particularly relates to an organic fertilizer with an insecticidal effect for producing potato breeder seeds.

Background

The potato stock is the preferable potato seed for modern potato planting, and is obtained by screening high-quality potato seeds, detoxifying the potato seeds, performing virus detection, starting tissue culture and rapid propagation to obtain tissue culture seedlings, performing water culture on the tissue culture seedlings to form strong seedlings, and cutting 10-15cm long high-strong potato seedlings for field planting after 10-15 days. At present, the original stock is obtained by cutting the detoxified seedling on a special substrate in a greenhouse for culture. Because the soil is directly used for planting the detoxified seedlings, the detoxified seedlings are generally difficult to survive or have extremely low survival rate, so that the original stock seeds are generally planted and produced by planting the detoxified seedlings by using a composite matrix consisting of vermiculite, organic fertilizer, soil and the like in an insect-proof net room at present.

The pest control in the breeding process of the original breeder seeds mainly controls aphids and early and late blight. Potato diseases represented by potato late blight and potato early blight seriously affect the yield and quality of potatoes, and become key and difficult points of prevention and control of the potato diseases and pests. Regularly and irregularly spraying, preventing, controlling and preventing imidacloprid, acetamiprid, pirimicarb, abamectin and other medicaments inside and outside a net room and in the surrounding environment, and timely treating the imidacloprid in the aphid migration hazard period; the metalaxyl manganese zinc, the jarosite, the Yinfari, the fast inhibitor, the Kelu and other medicaments are alternately sprayed to prevent, control and prevent the early and late blight. Although the traditional pesticide has a certain effect on preventing and treating plant diseases and insect pests, improper dosage or improper type of the traditional pesticide easily causes phytotoxicity, crops become more sensitive and are easy to generate drug resistance, and meanwhile, the potential threat of pesticide residues to the environment and human beings cannot be ignored.

The organic fertilizer is a fertilizer containing organic substances, which can provide various inorganic nutrients and organic nutrients for crops and can also fertilize and improve soil. Most of them are made of local materials by farmers and accumulated by oneself. Urban pollutants can also be changed into organic fertilizers through treatment. The organic fertilizer replaces inorganic fertilizer or is mixed with the inorganic fertilizer for application, so that the environmental problem can be effectively improved, and the environment and production waste can be effectively utilized to change waste into valuable. Various special crops such as sophora alopecuroide, purslane, selenium-containing fructus trichosanthis, medlar, narrow-leaved oleaster and the like are grown or planted in various areas of Ningxia, wastes generated in the processing process can increase the processing cost and cause environmental burden, and the inherent value of the crops needs to be reasonably processed and developed.

Disclosure of Invention

In order to solve the technical problems, the invention provides an organic fertilizer for producing potato breeder seeds, which is prepared from the following raw materials:

5-10 parts of sophora alopecuroide, 7-10 parts of selenium-containing fructus trichosanthis stem, 10-15 parts of waste selenium-containing fructus trichosanthis, 10-15 parts of medlar branches and leaves, 10-15 parts of purslane, 3-5 parts of broom cypress, 10-15 parts of oleaster processing waste, 5-10 parts of waste vinasse, 5-7 parts of plant ash, 10-20 parts of manure, 5-10 parts of a solid culture medium and 1-2 parts of a fermentation microbial inoculum;

the manure is at least one of chicken manure, pig manure, cow manure, rabbit manure and horse manure;

the solid culture medium is formed by crushing the waste solid culture medium containing the residual seedlings after the potato seedlings are cut in the culture process;

the fermentation microbial inoculum consists of yeast, lactic acid bacteria, bacillus, actinomycetes and azotobacter according to the ratio of 1.5:1.5:1.2:1.2: 1;

the fermentation method comprises the following specific steps:

(1) sterilizing the processing waste of the sophora alopecuroide, the selenium-containing fructus trichosanthis stem and leaf, the waste selenium-containing fructus trichosanthis, the branches and leaves of the Chinese wolfberry, the purslane, the broom cypress and the oleaster, crushing the disinfected processing waste into 80-100 meshes, and mixing the crushed processing waste and the crushed processing waste;

(2) adding water 1-1.5 times (v: m) of the total weight of the materials in the step (1), adding waste vinasse, and performing ultrasonic treatment for 6-8h at 30-45 ℃ under the conditions of 20-30KHz and 18-20 KW;

(3) adding plant ash, manure, solid culture medium and fermentation bacteria agent, mixing, piling into long-strip stacks, turning over for the first time after 7-10 days, fermenting for 10-15 days to reach a thoroughly decomposed state, and naturally cooling for use.

Has the advantages that:

(1) the organic fertilizer used in the invention is added with the culture medium discarded in the process of producing the seedlings, the culture process of the original stock can continuously utilize the rest nutrient substances in the culture medium, and the rest seedlings in the culture medium can release N, P, K and other elements after being fermented, and can be absorbed by the seedlings as part of the organic fertilizer; in addition, the agar particles have strong water binding capacity, the water containing capacity of the soil can be improved after the fertilizer enters the soil, and meanwhile, the solid culture medium particles with a certain shape can also play a role in increasing air permeability;

(2) the method utilizes the waste culture medium, realizes the harmless treatment of the culture medium, saves the post-treatment cost of the waste culture medium, and reduces the problem of environmental pollution caused by post-treatment;

(3) the organic fertilizer can effectively reduce the dosage of the insecticide by 25-35%, effectively reduce the harm of the insecticide to application personnel and environment, avoid crop phytotoxicity and drug resistance, and reduce the risk of pesticide residue.

(4) The organic fertilizer is used as a fertilizer for producing potato breeder seeds, can improve the selenium content in the breeder seeds by about 10-15%, and provides the nutritive value and the added value of crops.

(5) The addition of the oleaster processing waste and the vinasse waste in the organic fertilizer can improve the bactericidal performance of the fertilizer, has obvious positive effects on crop disease and pest resistance, reduces the disposal cost of industrial waste and the caused environmental burden, and changes waste into valuable.

Drawings

FIG. 1 is a bar graph of relative survival;

FIG. 2, bar graph of relative nodule rate;

FIG. 3, relative mean single particle weight bar graph;

fig. 4, bar graph of relative selenium content.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present patent and are not intended to limit the present invention.

Basically, this example 1 cultivation of tissue culture seedlings of Potato

(1) Shoot tip seedling culture

Selecting healthy potato, treating terminal bud or lateral bud together with partial petiole and stem segment in 70% alcohol for 30s, soaking in 10% bleaching powder solution for 5-10min, and washing with sterile water twice or three times;

after disinfection, the stem bud is peeled off under a binocular dissecting mirror of 10-40 times, the stem bud is pressed by a pair of tweezers on one hand, the young leaf and the large leaf primordium are peeled off by a dissecting needle on the other hand until a bright growing point is exposed, the small stem tip with one or two leaf primordiums is cut off by a dissecting knife and is quickly inoculated on a stem tip seedling culture medium for culture, the culture temperature is (25 +/-2) DEG C, the illumination intensity is 1000lx 4 weeks before, the illumination intensity is increased to 2000 lx 3000lx 4 weeks after, the illumination is 16h every day, and the seedling is grown into a seedling after 4-6 weeks, namely a stem tip seedling;

carrying out virus detection on the stem tip seedlings, and using the non-toxic seedlings for cutting and expanding propagation;

the stem tip seedling culture medium comprises the following components: MS culture +1.5 mg/L6-BA +0.1-0.5mg/L NAA +3mg/L penicillin +80mg/L sodium hypochlorite + 15g/L sucrose +3.5g/L agar, pH5.8;

(2) cutting propagation

Cutting a stem section (cut section) with one leaf from the nontoxic seedling to a propagation medium for culturing, continuously irradiating at 25-28 ℃ under the illumination condition of 1000-;

the propagation medium comprises: MS culture +1 mg/L6-BA +0.3mg/L NAA +3mg/L penicillin +80mg/L sodium hypochlorite + 15g/L sucrose +3.5g/L agar, pH5.8;

(3) tissue culture

Cutting a stem section (cutting section) with a leaf from the propagation-expanded seedling into a tissue culture medium for culture, culturing at 18-20 ℃, with the illumination intensity of 3000-4000 lx and the illumination time of about 20h every day, selecting potato seedlings with thick and strong roots and healthy colors after culturing for 20-30d, taking out, cutting off fibrous roots, washing off the culture medium attached to the roots, and transplanting the potato seedlings to produce potato breeder seeds.

The tissue culture medium consists of: MS culture +1 mg/L6-BA +0.3mg/L NAA +3mg/L penicillin +80mg/L sodium hypochlorite + 15g/L sucrose, pH5.8.

Example 2 an organic fertilizer for the production of potato breeder's seed

The organic fertilizer is prepared from the following raw materials:

5 parts of sophora alopecuroide, 7 parts of selenium-containing fructus amomi rattan, 10 parts of waste selenium-containing fructus amomi, 10 parts of medlar branches and leaves, 10 parts of purslane, 3 parts of broom cypress, 10 parts of oleaster processing waste, 5 parts of waste vinasse, 5 parts of plant ash, 10 parts of manure, 5 parts of a solid culture medium and 1 part of a zymogen agent;

the manure is prepared by mixing chicken manure, pig manure, cow manure, rabbit manure and horse manure according to the ratio of 1:1:1:1: 1;

the fermentation method comprises the following specific steps:

(2) adding water which is 1 time (v: m) of the total weight of the materials in the step (1), adding waste vinasse, and carrying out ultrasonic treatment for 6 hours under the conditions of 30 ℃, 20KHz and 18 KW;

(3) adding plant ash, manure, solid culture medium and fermentation bacteria agent, mixing uniformly, piling into long-strip stacks, turning over for the first time after 7 days, fermenting for 10 days to reach a rotten state, and naturally cooling for use.

Example 3 an organic fertilizer for the production of potato breeder's seed

The organic fertilizer is prepared from the following raw materials:

10 parts of sophora alopecuroide, 10 parts of selenium-containing fructus amomi rattan, 15 parts of waste selenium-containing fructus amomi, 15 parts of medlar branches and leaves, 15 parts of purslane, 5 parts of broom cypress, 15 parts of oleaster processing waste, 10 parts of waste vinasse, 7 parts of plant ash, 20 parts of manure, 10 parts of solid culture medium and 2 parts of zymogen agent;

the manure is chicken manure;

the fermentation method comprises the following specific steps:

(2) adding water which is 1.5 times (v: m) of the total weight of the materials in the step (1), adding waste vinasse, and carrying out ultrasonic treatment for 8 hours at 45 ℃, 30KHz and 20 KW;

(3) adding plant ash, manure, solid culture medium and fermentation bacteria agent, mixing uniformly, piling into long-strip stacks, turning over for the first time after 10 days, fermenting for 15 days to reach a rotten state, and naturally cooling for use.

Example 4 an organic fertilizer for the production of potato breeder's seed

The organic fertilizer is prepared from the following raw materials:

7 parts of sophora alopecuroide, 8 parts of selenium-containing fructus amomi rattan, 12 parts of waste selenium-containing fructus amomi, 12 parts of medlar branches and leaves, 12 parts of purslane, 4 parts of broom cypress, 12 parts of oleaster processing waste, 8 parts of waste vinasse, 6 parts of plant ash, 15 parts of manure, 8 parts of a solid culture medium and 2 parts of a zymogen agent;

the manure is prepared by mixing pig manure and cow manure in equal amount;

the fermentation method comprises the following specific steps:

(2) adding water which is 1.2 times (v: m) of the total weight of the materials in the step (1), adding waste vinasse, and carrying out ultrasonic treatment for 7 hours at 37 ℃, 25KHz and 20 KW;

(3) adding plant ash, manure, solid culture medium and fermentation bacteria agent, mixing uniformly, piling into long-strip stacks, turning over for the first time after 8 days, fermenting for 13 days to reach a rotten state, and naturally cooling for use.

Example 5A transplanting seedbed for producing potato stock

(1) Seedbed arrangement

The transplanting seedbed is divided into three layers: the bottom layer is nutrient soil with the thickness of 10cm, the middle layer is solid culture medium particles with the thickness of 2cm, and the upper layer is vermiculite with the thickness of 4 cm;

after the nutrient soil is laid, each 667m²Using 50% phoxim EC 250ml, 72% agricultural streptomycin SPX 250ml, 50% carbendazim WP 250g or 75% chlorothalonil WP 250g to process soil, then rotary tillage, rolling and leveling;

paving a 40-mesh nylon screen on the leveled soil, and paving solid culture medium particles on the screen;

spreading 20 mesh nylon screen on the solid culture medium particles, spreading vermiculite on the screen, each 667m ²10 kg of nitrogen, phosphorus and potassium compound fertilizer is applied into the vermiculite, and the mixture is fully stirred and uniformly mixed;

(2) nutrient soil

The nutrient soil consists of 70 parts of soil part and 55 parts of organic fertilizer;

the organic fertilizer is the organic fertilizer prepared in the embodiment 2-4;

the soil part consists of natural soil and soil in which gramineous plants are planted according to a ratio of 3.5: 0.75;

(3) middle layer solid culture medium particle

The middle-layer solid culture medium particles are prepared by removing residual seedlings from the solid culture medium left after the cutting in the step (2) and the solid culture medium left after the cutting in the step (3) in the embodiment 1 and granulating;

the particles are spherical particles with the diameter of about 0.5 cm;

the solid culture medium particles can be recycled for 2 times.

Example 6A method for producing a stock seed of potato

(1) And (3) disinfection: thoroughly disinfecting and sterilizing soil and matrix by using a disinfectant before transplanting, and strictly preventing the soil from spreading diseases and insect pests;

(2) watering: uniformly watering the vermiculite one day before transplanting to ensure that the water holding capacity of the vermiculite reaches a saturated state;

(3) transplanting: the potato seedlings prepared in example 1 were horizontally planted in vermiculite on the seedbed described in example 5, preferably with 2-3 leaves exposed at the head and with a row spacing of 3X 10cm (280-²）；

(4) Watering, pesticide application and film covering: uniformly watering the seedlings after transplanting to ensure that the seedlings are fully contacted with the seedbed; spraying 52.5% of fast-cleaning WP 1800-time liquid or 70% of mancozeb WP 1000-time liquid for sterilization and protection, then building a small shed for covering a film, covering a sunshade net, preserving moisture and heat, and ensuring that transplanted detoxified seedlings survive;

(5) temperature management: keeping the temperature in the greenhouse at 23-25 ℃ within 7-10 days after transplanting; when 3-5 new roots grow on the seedlings and the overground part has obvious growth vigor, the film is removed, the ventilation and light transmission conditions are enhanced, and the growth of the seedlings is promoted; uncovering the sunshade net 2-3 days after stem pressing, uncovering the sunshade net 5 pm in sunny days, and covering the sunshade net again about 10 am in the next day to prevent direct illumination and sun drying, and uncovering the sunshade net completely 3-5 days repeatedly; after the film is uncovered and the net is uncovered, 500-600 times of metalaxyl manganese zinc 58% wettable powder is sprayed, 52.5% fast-curing WP1800 times of liquid and silver legislation are alternately sprayed for preventing, controlling and preventing early and late blight every 7-10 days, the dosage of the metalaxyl manganese zinc 58% wettable powder is 52 g/mu/time, the dosage of the 52.5% fast-curing WP is 30 g/mu/time, and the dosage of the silver legislation is 40 mL/mu/time;

(5) water content management: replenishing water in time according to the moisture condition of the seedbed before uncovering the film, wherein the water replenishing time is carried out in the morning or evening, so that the water holding capacity of the substrate is kept at about 85 percent; water is replenished once before and after stem pressing, and then water is replenished in time according to the moisture condition of the matrix, so that the water holding capacity of the matrix is kept at 60-70%; stopping watering 10-15 days before harvesting to promote potato skin aging.

(6) Pressing stems: in order to increase the number of stolons and increase the number of single-plant tuber bearing, when seedlings grow to about 5cm, 1-2 branches of the stems are pressed into a matrix, so that 2-3 leaves are exposed at the seedling heads; squeezing is preferably performed in the afternoon.

(7) Controlling overgrowth: when the plant is overgrown in nutrition, the plant height is 20-25 cm and the optical layer coverage rate is more than 95%, spraying paclobutrazol or chlormequat chloride with the concentration of 0.2% for 1-2 times to control overground part overgrowth;

(8) harvesting: harvesting in time when the leaves at the middle and lower parts of the plant become yellow, sterilizing with cyazofamid (Kejia) or copper sulfate 10-15 days before harvesting, and stopping watering.

Example 7 Effect test

The potato seedling cultivation methods adopted in the following examples are all the methods described in example 1, the original seed production methods are all the methods described in example 6, the adopted transplanting seedbed is the seedbed described in example 5, and only organic fertilizers in the seedbed are different;

experimental example 1: the organic fertilizer described in example 2 was used;

experimental example 2: the organic fertilizer described in example 3 was used;

experimental example 3: the organic fertilizer described in example 4 was used;

comparative example 1: the organic fertilizer used in the embodiment 2 is the organic fertilizer without the solid culture medium, and the rest is the same as the embodiment 2;

comparative example 2: the organic fertilizer used is the organic fertilizer of the embodiment 3 without the solid culture medium, and the rest is the same as the embodiment 3;

comparative example 3: the organic fertilizer used is the organic fertilizer of the embodiment 4 without the solid culture medium, and the rest is the same as the embodiment 4;

the experimental results show that the experimental examples have less obvious change on the survival rate of the proportion, but the number of grains and the yield can be improved by 5-10%, so that the soil fertility can be improved by adding the waste culture medium in the organic fertilizer fermentation process, the yield of the original seeds is improved to a certain extent, the production cost of the original seeds with unit yield is reduced on one hand, and the problem of post-treatment of the waste culture medium is solved on the other hand. Therefore, after the waste solid culture medium is used for fermenting the organic fertilizer, the residual nutrient substances, hormone and the like in the waste solid culture medium can be reused, the water-holding capacity of the agar particles is strong, the water-containing capacity of the soil can be improved, and the solid particles in a certain shape can also play a role in increasing the air permeability.

EXAMPLE 8 Effect test

The following examples are not specifically described, and the potato seed seedling cultivation methods used are the methods described in example 1, the stock seed production methods are the methods described in example 6, and the transplanted seedbed used is the seedbed described in example 5, and the potato stock seed production is carried out, with the following differences only;

experimental example 1: the organic fertilizer described in example 4 was used;

comparative example 1: replacing the waste selenium shaggua and the selenium shaggua vines in the organic fertilizer raw materials in the embodiment 4 with common watermelons and melon vines, and the rest is the same as the embodiment 4;

comparative example 2: the oleaster processing waste and the waste vinasse in the organic fertilizer raw material in the embodiment 4 are replaced by the same amount of straws, and the rest is the same as the embodiment 4;

comparative example 3: the organic fertilizer raw material in the embodiment 4 is replaced by a raw material composition A: 23 parts of straw, 24 parts of rice bran, 20 parts of pine branches, 8 parts of bean dregs, 6 parts of plant ash, 15 parts of manure, 8 parts of solid culture medium and 2 parts of zymogen; the preparation method is not changed; the dosage of the 58% wettable metalaxyl manganese zinc powder in the method for producing the original breeder seeds (example 6) is 52 g/mu/time, the dosage of 52.5% fast-inhibiting pesticide is 30 g/mu/time, and the dosage of the silver faradaic is 40 mL/mu/time, and is increased to 60 g/mu/time, 30 g/mu/time and 45 mL/mu/time of 58% wettable metalaxyl manganese zinc powder;

comparative example 4: replacing the organic fertilizer raw material in the example 4 with a raw material composition A, wherein the dosage of 58% wettable metalaxyl manganese-zinc powder in the method for producing the original seed (example 6) is 52 g/mu/time, the dosage of 52.5% fast-acting pesticide is 30 g/mu/time, and the dosage of the silver fargesic is 40 mL/mu/time, and is increased to 70 g/mu/time, the dosage of 52.5% fast-acting pesticide is 35 g/mu/time, and the dosage of the silver fargesic is 50 mL/mu/time;

comparative example 5: replacing the organic fertilizer raw material in the example 4 with a raw material composition A, wherein the dosage of the 58% wettable metalaxyl manganese-zinc powder in the method for producing the original seed (example 6) is 52 g/mu/time, the dosage of the 52.5% fast-acting pesticide is 30 g/mu/time, and the dosage of the silver fargeil is 40 mL/mu/time, and is increased to 80 g/mu/time, the dosage of the 52.5% fast-acting pesticide is 40 g/mu/time, and the dosage of the silver fargeil is 60 mL/mu/time;

respectively counting the survival rate, the number of nodules, the average unit weight of the grains and the selenium content of the experimental example 1 and the comparative examples 1-5, taking the statistical data of the experimental example 1 as a reference value of 100%, and calculating the relative survival rate, the relative number of the nodules, the relative average unit weight of the grains and the relative selenium content of the comparative examples 1-5 relative to the experimental example 1, wherein the results are shown in figures 1-4;

relative survival = comparative survival/experimental example 1 survival;

relative nodule count = comparative nodule count/experimental example 1 nodule count;

relative average unit particle weight = comparative example average unit particle weight/experimental example 1 average unit particle weight;

relative selenium content = comparative selenium content/selenium content of experimental example 1;

as can be seen from the figures 1-4, compared with the comparative example 1, the selenium content in the original seeds can be improved by about 10-15% by adding the selenium-containing muskmelon stems and the waste selenium-containing muskmelons into the organic fertilizer in the experimental example 1; compared with the data of the comparative example 2 and the observation of the original seed culture site, the method has the advantages that the sterilization effect of the fertilizer can be improved after the oleaster waste and the waste vinasse are added into the fertilizer, and the oleaster waste and the waste vinasse are presumed to promote the release of sterilization components in the raw materials in the ultrasonic process, so that the sterilization effect is enhanced; as can be seen from the experimental example 1 and the comparative examples 3-5, the organic fertilizer with insecticidal effect of the invention and the conventional organic fertilizer are used for cultivating the potato breeder's stock, and the invention can reduce the dosage of insecticide by 25-35% to achieve the same survival rate and yield.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the patent. It should be noted that, for those skilled in the art, various changes, combinations and improvements can be made in the above embodiments without departing from the patent concept, and all of them belong to the protection scope of the patent. Therefore, the protection scope of this patent shall be subject to the claims.

Claims

1. An organic fertilizer for producing potato breeder's seeds is characterized in that the organic fertilizer is prepared from the following raw materials:

the preparation method of the organic fertilizer comprises the following steps:

2. The organic fertilizer for producing potato breeder's seeds of claim 1, characterized in that said organic fertilizer is prepared from the following raw materials:

the preparation method of the organic fertilizer comprises the following steps:

3. The organic fertilizer for producing potato breeder's seeds of claim 1, characterized in that said organic fertilizer is prepared from the following raw materials:

the manure is chicken manure;

the preparation method of the organic fertilizer comprises the following steps:

4. The organic fertilizer for producing potato breeder's seeds of claim 1, characterized in that said organic fertilizer is prepared from the following raw materials:

the manure is prepared by mixing pig manure and cow manure in equal amount;

the preparation method of the organic fertilizer comprises the following steps: