CN115802889A - 生物样品低温储存 - Google Patents

生物样品低温储存 Download PDF

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Publication number
CN115802889A
CN115802889A CN202180029526.0A CN202180029526A CN115802889A CN 115802889 A CN115802889 A CN 115802889A CN 202180029526 A CN202180029526 A CN 202180029526A CN 115802889 A CN115802889 A CN 115802889A
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cells
concentration
cell
cryopreservation
solution
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CN202180029526.0A
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English (en)
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李宗海
王华茂
高慧萍
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Clegg Medical Co ltd
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Clegg Medical Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/461Cellular immunotherapy characterised by the cell type used
    • A61K39/4611T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/463Cellular immunotherapy characterised by recombinant expression
    • A61K39/4631Chimeric Antigen Receptors [CAR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/60Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62DMSO
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/70Undefined extracts
    • C12N2500/80Undefined extracts from animals
    • C12N2500/84Undefined extracts from animals from mammals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

本发明涉及细胞生物学领域。本发明提供了低温储存生物样品的冻存液,特别是低温储存单采术样品的细胞的冻存液。

Description

PCT国内申请,说明书已公开。

Claims (16)

  1. PCT国内申请,权利要求书已公开。
CN202180029526.0A 2020-04-21 2021-04-21 生物样品低温储存 Pending CN115802889A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2020103165603 2020-04-21
CN202010316560 2020-04-21
PCT/CN2021/088745 WO2021213446A1 (zh) 2020-04-21 2021-04-21 生物样品低温储存

Publications (1)

Publication Number Publication Date
CN115802889A true CN115802889A (zh) 2023-03-14

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CN202180029526.0A Pending CN115802889A (zh) 2020-04-21 2021-04-21 生物样品低温储存

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US (1) US20230148586A1 (zh)
CN (1) CN115802889A (zh)
WO (1) WO2021213446A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023223291A1 (en) * 2022-05-20 2023-11-23 Takeda Pharmaceutical Company Limited Methods of producing engineered immune cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102986648A (zh) * 2012-10-30 2013-03-27 中国林业科学研究院森林生态环境与保护研究所 松材线虫的玻璃化冷冻保存及解冻复苏方法
CN104145943A (zh) * 2014-07-21 2014-11-19 重庆市红汇脐血干细胞中心有限公司 一种人脐带华通氏胶组织的冻存保护液及其制备与应用
CN108235981A (zh) * 2016-12-23 2018-07-03 西比曼生物科技(上海)有限公司 一种可临床使用的细胞冻存液
CN109315386A (zh) * 2018-12-12 2019-02-12 中南大学湘雅三医院 一种可用于造血干细胞或淋巴细胞的冻存液及冻存方法
WO2020033959A1 (en) * 2018-08-10 2020-02-13 Predictive Technology Group, Inc. Cryopreservation medium for umbilical cord mscs derived from wharton's jelly

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104222069B (zh) * 2014-08-27 2016-06-29 中国人民解放军军事医学科学院野战输血研究所 红系祖细胞冻存液及其应用
CN107581186A (zh) * 2017-10-16 2018-01-16 北京壹美源生物科技有限公司 细胞冻存液及其制备方法与应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102986648A (zh) * 2012-10-30 2013-03-27 中国林业科学研究院森林生态环境与保护研究所 松材线虫的玻璃化冷冻保存及解冻复苏方法
CN104145943A (zh) * 2014-07-21 2014-11-19 重庆市红汇脐血干细胞中心有限公司 一种人脐带华通氏胶组织的冻存保护液及其制备与应用
CN108235981A (zh) * 2016-12-23 2018-07-03 西比曼生物科技(上海)有限公司 一种可临床使用的细胞冻存液
WO2020033959A1 (en) * 2018-08-10 2020-02-13 Predictive Technology Group, Inc. Cryopreservation medium for umbilical cord mscs derived from wharton's jelly
CN109315386A (zh) * 2018-12-12 2019-02-12 中南大学湘雅三医院 一种可用于造血干细胞或淋巴细胞的冻存液及冻存方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
沈建良等: "造血干细胞低温保存与复温操作规程(讨论稿)", 转化医学杂志, vol. 4, no. 5, 31 October 2015 (2015-10-31), pages 316 *

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US20230148586A1 (en) 2023-05-18
WO2021213446A1 (zh) 2021-10-28

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