CN115517269B - Rhododendron seedling raising method - Google Patents

Rhododendron seedling raising method Download PDF

Info

Publication number
CN115517269B
CN115517269B CN202211248120.4A CN202211248120A CN115517269B CN 115517269 B CN115517269 B CN 115517269B CN 202211248120 A CN202211248120 A CN 202211248120A CN 115517269 B CN115517269 B CN 115517269B
Authority
CN
China
Prior art keywords
rhododendron
parts
seed soaking
substrate
soaking agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202211248120.4A
Other languages
Chinese (zh)
Other versions
CN115517269A (en
Inventor
马宏
万友名
李金仙
李正红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences
Original Assignee
Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences filed Critical Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences
Priority to CN202211248120.4A priority Critical patent/CN115517269B/en
Publication of CN115517269A publication Critical patent/CN115517269A/en
Application granted granted Critical
Publication of CN115517269B publication Critical patent/CN115517269B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08—Immunising seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/60—Flowers; Ornamental plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention provides a rhododendron seed soaking agent and a rhododendron seedling raising method, and belongs to the technical field of plant propagation. The rhododendron seed soaking agent disclosed by the invention comprises the following raw materials: 2-6 parts of vitamin C, 2-6 parts of gibberellin, 1-5 parts of chitosan, 10-25 parts of hedyotis diffusa extract and 10-25 parts of cocklebur fruit extract. According to the invention, the rhododendron seed soaking agent is adopted to soak the rhododendron seeds, then the rhododendron seeds are sown on the surface of the matrix, covered with soil and placed under natural illumination until the rhododendron germinates, and shading treatment is carried out after germination, so that the emergence rate and the seedling rate of the rhododendron can be effectively improved, the large-scale reproduction of the rhododendron is realized, and the production and popularization are facilitated.

Description

Rhododendron seedling raising method
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a rhododendron seed soaking agent and a rhododendron seedling raising method.
Background
Rhododendron (school name: rhododendringsimsii ipiland), dicotyledonous plants, rhododendron, evergreen shrubs, deciduous shrubs of rhododendron. The other name is azalea and punica granatum. The azalea flowers in the spring, 2-6 flowers per cluster, the corolla funnel shape, red, light red, apricot red, snow green, white and the like, and the flower color is luxuriant and gorgeous. The plant is indicated for typical acid soil in the south and the south of China under the mountain brush or pine forest with the altitude of 500-1200 meters. The rhododendron whole plant is used for medicine: has effects in promoting qi and blood circulation, tonifying deficiency, and treating cough due to internal injury, deafness due to kidney deficiency, menoxenia, and rheumatism. The flower crowns are bright red, so that the flower crowns are famous flower plants, have higher ornamental value and are cultivated in parks all over the world. The rhododendron flower is used as a province in Jiangxi, anhui and Guizhou China, and the city of the rhododendron flower is up to seven to eight. Azalea was rated as six of ten flowers in China 5 months in 1985.
The breeding of azalea can be realized by five methods of cutting, grafting, layering, separating plants and sowing. The seed propagation method of the azalea is simple and easy to implement, but in the prior art, the emergence rate of the seeds in the seed propagation of the azalea is low, and the time from the emergence of the plants to the seedling formation is long. Therefore, the seed propagation method is less used for nursery mass production to grow seedlings.
Disclosure of Invention
Therefore, the invention aims to provide the rhododendron seed soaking agent which can effectively promote germination and rooting of seeds and improve the emergence rate of the rhododendron seeds.
The invention also aims to provide a seedling raising method of azalea, which can effectively improve the emergence rate and the seedling rate of azalea and realize the large-scale propagation of azalea.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a rhododendron seed soaking agent which comprises the following raw materials in parts by mass: 2-6 parts of vitamin C, 2-6 parts of gibberellin, 1-5 parts of chitosan, 10-25 parts of hedyotis diffusa extract and 10-25 parts of cocklebur fruit extract.
Preferably, the hedyotis diffusa is soaked in water for 10 to 50 minutes and is extracted by hot water reflux at 80 to 90 ℃ for 20 to 50 minutes according to the feed liquid ratio of 1:15 to 40g/mL, and the hedyotis diffusa extract is obtained.
Preferably, the fructus xanthil is crushed, water is added according to the feed liquid ratio of 1:10-30 g/mL, ultrasonic pretreatment is carried out for 20-40 min, and reflux extraction is carried out for 20-40 min in hot water at 80-90 ℃ to obtain fructus xanthil extract.
Preferably, the ultrasonic pretreatment conditions are as follows: the power is 600-650W, and the temperature is 30-60 ℃.
The invention also provides application of the rhododendron seed soaking agent in rhododendron seedling culture.
The invention also provides a rhododendron seedling raising method, which comprises the following steps:
(1) Preparing a substrate, filling the substrate into a container, compacting the substrate tightly, and scenting the substrate with water to saturate the substrate;
(2) Diluting the rhododendron seed soaking agent with water, and soaking rhododendron seeds for 20-60 min;
(3) Sowing the rhododendron seeds on the surface of the matrix, and covering soil for 2-7 mm;
(4) After sowing, the seeds are placed under natural illumination until the azalea germinates, and shading treatment is carried out after the germination.
Preferably, the matrix comprises the following raw materials in parts by mass: 20-30 parts of peat soil, 5-12 parts of river sand, 2-6 parts of pine needle and 0.1-0.5 part of radix isatidis powder.
Preferably, 2-10 liters of water is added into each liter of rhododendron seed soaking agent.
Preferably, the seeding density is 1-5 grains/cm 2 。
Preferably, in the step (4), the temperature is 20-25 ℃, the humidity is 80-90%, and the shading rate of the shading treatment is 85-95%.
The invention has the beneficial effects that:
according to the invention, the hedyotis diffusa extract and the xanthium sibiricum extract are respectively extracted, and reasonably compatible with the vitamin C, the gibberellin and the chitosan to obtain the rhododendron seed soaking agent, so that a natural biological activity protective film can be formed on the surface layer of the rhododendron seed, the activity of various enzymes contained in the rhododendron seed is increased, the germination and rooting of the rhododendron seed are further effectively promoted, the dormancy of the seed is relieved, and the emergence rate of the rhododendron seed is effectively improved.
According to the invention, the rhododendron seeds are soaked by the seed soaking agent, then the rhododendron seeds are sown on the surface of the matrix, and the soil is covered, so that the high germination rate and the high seedling rate of the rhododendron are realized by controlling the conditions of moisture, temperature, humidity, light and the like, wherein the germination rate is as high as 93%, the seedling rate is as high as 89%, the large-scale propagation of the rhododendron is realized, and the large-scale production and popularization are facilitated.
Detailed Description
The invention provides a rhododendron seed soaking agent which comprises the following raw materials in parts by mass: 2-6 parts of vitamin C, 2-6 parts of gibberellin, 1-5 parts of chitosan, 10-25 parts of hedyotis diffusa extract and 10-25 parts of cocklebur fruit extract.
Preferably, the rhododendron seed soaking agent comprises the following raw materials in parts by mass: 3-5 parts of vitamin C, 3-5 parts of gibberellin, 2-4 parts of chitosan, 15-20 parts of hedyotis diffusa extract and 15-20 parts of cocklebur fruit extract.
More preferably, the rhododendron seed soaking agent comprises the following raw materials in parts by mass: 4 parts of vitamin C, 4 parts of gibberellin, 3 parts of chitosan, 16 parts of hedyotis diffusa extract and 18 parts of cocklebur fruit extract.
The common Hedyotis herb is Hedyotis diffusa (academic name: hedyotis diffusa Willd.) and the plant of Rubiaceae genus plant, annual draping herb, 15-50 cm higher. Slender root, branch, white flower. The stems are slightly square or flat cylindrical, smooth and hairless, and the branches are sent out from the basal part. Leaf pairing; no handle; the blade is linear to linear and needle-shaped, the length is 1-3.5 cm, the width is 1-3 mm, the tip of the front end is smooth, the lower surface is sometimes rough, and the lateral pulse is not obvious; the base of the leaf supporting membranous substance is combined into a sheath shape, the length of the leaf supporting membranous substance is 1-2 mm, and the tip of the leaf supporting membranous substance is a sharp point. The invention does not limit the specific source of the hedyotis diffusa. The whole plant of the hedyotis diffusa is preferably fresh hedyotis diffusa.
According to the invention, the hedyotis diffusa is soaked in water for 10 to 50 minutes according to the feed liquid ratio of 1:15 to 40g/mL, and the hot water at 80 to 90 ℃ is used for reflux extraction for 20 to 50 minutes, so that the hedyotis diffusa extract is obtained. As an alternative implementation mode, the invention pulverizes the whole plant of the hedyotis diffusa into segments with the length of 1-3 cm, and then water is added for reflux extraction to obtain the hedyotis diffusa extract. The feed liquid ratio is preferably 1:20-25 g/mL, the soaking time is preferably 20-35 min, and the reflux extraction time is preferably 25-35 min.
Fructus Xanthii belongs to fruit with total bud of Xanthium sibiricum of Compositae and Xanthium, and has yellow brown or yellow green surface, hook thorn on the whole, 2 thicker thorns on the top, separating or connecting, and fruit stalk mark on the base. The invention does not limit the specific source of fructus Xanthii.
Grinding fructus xanthil, adding water according to a feed liquid ratio of 1:10-30 g/mL, carrying out ultrasonic pretreatment for 20-40 min, and carrying out reflux extraction in hot water at 80-90 ℃ for 20-40 min to obtain fructus xanthil extract. As an alternative implementation mode, the invention washes the collected fructus xanthil material, then places the fructus xanthil material in the shade under natural conditions, and then pulverizes the fructus xanthil material to prepare the extracting solution. The ratio of the feed to the liquid is preferably 1:18-24 g/mL, the ultrasonic pretreatment time is preferably 29-34 min, and the reflux extraction time is preferably 26-32 min.
In the invention, ultrasonic pretreatment conditions of the fructus xanthil extract are as follows: the power is 600-650W, and the temperature is 30-60 ℃. The power is preferably 630 to 640W and the temperature is preferably 40 to 51 ℃.
The preparation method of the rhododendron seed soaking agent comprises the following steps: dissolving gibberellin in water to obtain gibberellin aqueous solution; the vitamin C and chitosan are directly added into the mixed solution of the cocklebur fruit extract and the hedyotis diffusa extract, and are mixed with gibberellin solution after stirring and dissolving, so as to obtain the rhododendron seed soaking agent.
The feed liquid ratio of gibberellin to water is 1:10 to 50g/mL, preferably 1:20 to 45g/mL. The gibberellin aqueous solution is easy to decompose and is not suitable for long-term storage, and the gibberellin aqueous solution needs to be prepared at present.
The invention can effectively extract flavonoid, triterpenes, esters and other secondary biomasses from the hedyotis diffusa and the fructus xanthii, the obtained mixed solution of the extract is reasonably proportioned with the vitamin C, chitosan and gibberellin aqueous solution, the influence of single component on endogenous inhibitors of the rhododendron seeds can be eliminated, the activity of hydrolase in the rhododendron seeds is improved, the decomposition of nutrient substances is promoted, and the germination of the rhododendron seeds is promoted.
The invention also provides application of the rhododendron seed soaking agent in rhododendron seedling culture. The azalea of the present invention includes, but is not limited to, rhododendron yunnanense, rhododendron annum, rhododendron cosmesis, rhododendron alpine, rhododendron grandiflorum.
The invention provides a rhododendron seedling raising method, which comprises the following steps:
(1) Preparing a substrate, filling the substrate into a container, compacting the substrate tightly, and scenting the substrate with water to saturate the substrate;
(2) Diluting the rhododendron seed soaking agent with water, and soaking the rhododendron seeds for 20-60 min;
(3) Sowing the rhododendron seeds on the surface of the matrix, and covering soil for 2-7 mm;
(4) After sowing, the seeds are placed under natural illumination until the azalea germinates, and shading treatment is carried out after the germination.
The matrix provided by the invention comprises the following raw materials in parts by mass: 20-30 parts of peat soil, 5-12 parts of river sand, 2-6 parts of pine needle and 0.1-0.5 part of radix isatidis powder. Preferably, the matrix comprises the following raw materials in parts by mass: 22-26 parts of peat soil, 8-11 parts of river sand, 3-5 parts of pine needle and 0.2-0.3 part of radix isatidis powder.
The peat soil is preferably soil with a peat layer formed by accumulating a large amount of insufficiently decomposed plant residues under the condition of hypoxia because of long-term water accumulation and dense aquatic vegetation in a low-level land of river and lake sediments and valley lands between mountains. The river sand is preferably non-metal ore which is produced by repeatedly impacting and rubbing natural stone for a long time under the action of water in a natural state, has complex components, has certain smoothness on the surface and contains a large amount of impurities. The pine needles of the invention are preferably dry pine needles which fall off naturally, and the pine needles are crushed for use. The isatis root powder is preferably a powder obtained by grinding isatis root which is a traditional Chinese medicine.
According to the invention, the isatis root powder is added into the matrix, so that the bacterial/viral microbial activity in the matrix can be effectively inhibited, the stress resistance of the rhododendron seedlings is improved, and a higher seedling rate is ensured.
The present invention fills the substrate into a container, compacts the substrate tightly, and scents the substrate with water to saturate the substrate, so that the substrate can reach the maximum water holding capacity.
The rhododendron seed soaking agent is diluted by adding water, and the rhododendron seeds are soaked for 20-60 min under the normal temperature condition; 2-10 liters, preferably 3-6 liters, of water is added into each liter of rhododendron seed soaking agent. The soaking time is preferably 30-45 min.
The invention directly spreads the rhododendron seeds after seed soaking treatment to the surface of a matrix, wherein the sowing density is 1-5 grains/cm 2 Preferably 2 to 3 grains/cm 2 . The rhododendron seeds are spread on the surface of the matrix and then covered with soil for 2-7 mm, preferably 3-5 mm. As an alternative embodiment, the soil of the present invention is a nutrient soil having fine particles.
After sowing, the sowing container is placed under natural illumination until the azalea germinates, wherein the placing temperature is 20-25 ℃, preferably 22-23 ℃, and the humidity is 80-90%, preferably 85-92%. After the rhododendron seeds germinate, the rhododendron seeds are subjected to shading treatment under the condition of unchanged temperature and humidity, wherein the shading rate of the shading treatment is 85-95%, and is preferably 89-92%.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
In the specific embodiment of the invention, the preparation method of the hedyotis diffusa extract comprises the following steps: pulverizing whole plant of herba Hedyotidis Diffusae into segments of about 2cm length, adding water according to a feed-liquid ratio of 1:25g/mL, soaking for 30min, and reflux extracting with hot water at 90deg.C for 30min to obtain herba Hedyotidis Diffusae extractive solution.
The preparation method of the cocklebur fruit extract comprises the following steps: washing the collected fructus Xanthii material, drying in the shade under natural condition, grinding, adding water according to a feed-liquid ratio of 1:20g/mL, pretreating with ultrasonic wave (600W, 50 ℃) for 30min, and reflux-extracting with hot water at 90deg.C for 30min to obtain fructus Xanthii extractive solution.
In the following examples, conventional methods are used unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1
The rhododendron seed soaking agent comprises the following raw materials: 5 parts of vitamin C, 5 parts of gibberellin, 4 parts of chitosan, 12 parts of hedyotis diffusa extract and 15 parts of cocklebur fruit extract.
The preparation method comprises the following steps: dissolving gibberellin in water (feed-liquid ratio 1:20 g/mL) to obtain gibberellin aqueous solution; mixing fructus Xanthii extract and herba Hedyotidis Diffusae extract, adding vitamin C and chitosan, stirring to dissolve, and mixing with gibberellin solution to obtain azalea seed soaking agent.
Example 2
This embodiment differs from embodiment 1 only in that: the rhododendron seed soaking agent comprises the following components: 4 parts of vitamin C, 4 parts of gibberellin, 3 parts of chitosan, 20 parts of hedyotis diffusa extract and 15 parts of cocklebur fruit extract.
Example 3
This embodiment differs from embodiment 1 only in that: the rhododendron seed soaking agent comprises the following components: 3 parts of vitamin C, 3 parts of gibberellin, 4 parts of chitosan, 10 parts of hedyotis diffusa extract and 20 parts of cocklebur fruit extract.
Comparative example 1
The difference between this comparative example and example 1 is that: does not contain the extract of the hedyotis diffusa.
Comparative example 2
The difference between this comparative example and example 1 is that: does not contain fructus Xanthii extract.
Comparative example 3
The difference between this comparative example and example 1 is that: the hedyotis diffusa extract is replaced by the chrysanthemum extract with the same amount.
Comparative example 4
The difference between this comparative example and example 1 is that: the fructus Xanthii extract is replaced with the same amount of flos Chrysanthemi extract.
Example 4
A rhododendron seedling raising method comprises the following steps:
(1) Preparing a substrate, filling the substrate into a tray, and pressing the substrate to be compact; placing the tray into a pool for scenting water so as to enable the matrix in the tray to reach the maximum water holding capacity;
(2) The rhododendron seed soaking agent in the embodiment 1 is adopted, water is added into each liter of rhododendron seed soaking agent for 4 liters, and rhododendron seeds are soaked for 30 minutes at normal temperature;
(3) Sowing rhododendron seeds on the surface of the matrix, wherein the sowing density is about 2-3 grains/cm 2 Covering the rhododendron seeds with fine nutrient soil for about 3mm after sowing the rhododendron seeds on the surface of the matrix;
(4) After sowing, the tray is placed under natural light (the temperature is 23 ℃ and the humidity is 92%) until the rhododendron germinates, and after the rhododendron seeds germinate, the rhododendron seeds are subjected to shading treatment under the condition that the temperature and the humidity are unchanged, wherein the shading rate is 92%.
The matrix in the step (1) is obtained by mixing 22 parts of peat soil, 10 parts of river sand, 3 parts of pine needles and 0.3 part of radix isatidis powder.
Example 5
This embodiment differs from embodiment 4 in that: in the step (2), each liter of rhododendron seed soaking agent is added with 8 liters of water, and rhododendron seeds are soaked for 25 minutes at normal temperature.
Example 6
This embodiment differs from embodiment 4 only in that: in step (3), the fine nutrient soil is covered by about 5mm.
Example 7
This embodiment differs from embodiment 4 only in that: in the step (4), the temperature is 20 ℃ and the humidity is 90%.
Example 8
This embodiment differs from embodiment 4 only in that: in the step (4), the light shielding rate was 95%.
Example 9
This embodiment differs from embodiment 4 only in that: in the step (4), the matrix in the step (1) consists of 30 parts of peat soil, 10 parts of river sand, 4 parts of pine needles and 0.5 part of radix isatidis powder.
Comparative example 5
This comparative example differs from example 4 only in that: omitting the step (2) and directly sowing the rhododendron seeds on the surface of the matrix.
Comparative example 6
This comparative example differs from example 4 only in that: step (2) is omitted, and rhododendron seeds are added into boric acid to be soaked for 24 hours.
Comparative example 7
This comparative example differs from example 4 only in that: the substrate in the step (1) does not contain isatis root powder.
Comparative example 8
This comparative example differs from example 4 only in that: the seed soaking agent prepared in the comparative example 1 is adopted in the step (2).
Comparative example 9
This comparative example differs from example 4 only in that: the seed soaking agent prepared in the comparative example 2 is adopted in the step (2).
Comparative example 10
This comparative example differs from example 4 only in that: the seed soaking agent prepared in the comparative example 3 is adopted in the step (2).
Comparative example 11
This comparative example differs from example 4 only in that: the seed soaking agent prepared in the comparative example 4 is adopted in the step (2).
The methods of examples 4 to 8 and comparative examples 5 to 11 were used to seed rhododendron grandiflorum seeds and rhododendron grandiflorum seeds, respectively, and the germination rate and the seedling rate were counted. The results are shown in Table 1.
TABLE 1 germination and seedling rate of Rhododendron simsii
Figure BDA0003886828300000081
As can be seen from Table 1, the invention is characterized in that the rhododendron seeds are soaked by the seed soaking agent, and the germination matrix of the rhododendron seeds is improved, so that the germination rate and the seedling rate of the rhododendron can be effectively improved, and the invention is beneficial to large-scale planting and popularization and utilization of the rhododendron.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (8)

1. The rhododendron seed soaking agent is characterized by comprising the following raw materials in parts by mass: 2-6 parts of vitamin C, 2-6 parts of gibberellin, 1-5 parts of chitosan, 10-25 parts of hedyotis diffusa extract and 10-25 parts of cocklebur fruit extract;
adding water into the hedyotis diffusa according to the feed liquid ratio of 1:15-40 g/mL, soaking for 10-50 min, and carrying out hot water reflux extraction at 80-90 ℃ for 20-50 min to obtain hedyotis diffusa extract;
grinding fructus Xanthii, adding water according to a feed liquid ratio of 1:10-30 g/mL, carrying out ultrasonic pretreatment for 20-40 min, and carrying out reflux extraction in hot water at 80-90 ℃ for 20-40 min to obtain the fructus Xanthii extract.
2. The seed soaking agent according to claim 1, wherein the ultrasonic pretreatment conditions are: the power is 600-650W, and the temperature is 30-60 ℃.
3. The use of the rhododendron seed soaking agent according to any one of claims 1-2 in rhododendron seedling.
4. The azalea seedling raising method is characterized by comprising the following steps of:
(1) Preparing a substrate, filling the substrate into a container, compacting the substrate tightly, and scenting the substrate with water to saturate the substrate;
(2) Diluting the rhododendron seed soaking agent according to any one of claims 1-2 with water, and soaking rhododendron seeds for 20-60 min;
(3) Sowing rhododendron seeds on the surface of a substrate, and covering soil for 2-7 mm;
(4) After sowing, the seeds are placed under natural illumination until the azalea germinates, and shading treatment is carried out after the germination.
5. The seedling raising method as claimed in claim 4, wherein the substrate comprises the following raw materials in parts by mass: 20-30 parts of peat soil, 5-12 parts of river sand, 2-6 parts of pine needles and 0.1-0.5 part of radix isatidis powder.
6. The method for raising seedlings according to claim 4, wherein 2-10 liters of water is added to each liter of rhododendron seed soaking agent.
7. The seedling raising method as set forth in claim 4, wherein the sowing density is 1 to 5 grains/cm 2 。
8. The method of raising seedlings according to claim 4, wherein in said step (4), the temperature is 20 to 25 ℃, the humidity is 80 to 90%, and the shading rate of the shading treatment is 85 to 95%.
CN202211248120.4A 2022-10-12 2022-10-12 Rhododendron seedling raising method Active CN115517269B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211248120.4A CN115517269B (en) 2022-10-12 2022-10-12 Rhododendron seedling raising method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211248120.4A CN115517269B (en) 2022-10-12 2022-10-12 Rhododendron seedling raising method

Publications (2)

Publication Number Publication Date
CN115517269A CN115517269A (en) 2022-12-27
CN115517269B true CN115517269B (en) 2023-06-23

Family

ID=84700946

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211248120.4A Active CN115517269B (en) 2022-10-12 2022-10-12 Rhododendron seedling raising method

Country Status (1)

Country Link
CN (1) CN115517269B (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532206A (en) * 1991-08-15 1996-07-02 Evans; Lloyd T. Method of treating plants or plant tissues with C-16,17-dihydro gibberellins
CN105960883A (en) * 2016-06-29 2016-09-28 固镇县华原家庭农场 Germination accelerating method for increasing pepper seed germination rate
CN108323509A (en) * 2018-01-22 2018-07-27 马鞍山市全润农业科技有限公司 A kind of seed soaking agent and preparation method thereof for vegetable seeds
CN108703161A (en) * 2018-07-18 2018-10-26 安徽省金海种业有限公司 A kind of paddy rice seed soaking agent
CN109362813A (en) * 2018-11-05 2019-02-22 贵州好菇粮农业科技有限公司 A kind of mushroom growth regulator and preparation method thereof
CN114009174A (en) * 2021-12-02 2022-02-08 广东石油化工学院 Method for promoting germination rate of pepper seeds
CN114982415A (en) * 2022-05-26 2022-09-02 黑龙江省林业科学研究所 Method for improving germination rate of alpine rhododendron and seedling raising method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532206A (en) * 1991-08-15 1996-07-02 Evans; Lloyd T. Method of treating plants or plant tissues with C-16,17-dihydro gibberellins
CN105960883A (en) * 2016-06-29 2016-09-28 固镇县华原家庭农场 Germination accelerating method for increasing pepper seed germination rate
CN108323509A (en) * 2018-01-22 2018-07-27 马鞍山市全润农业科技有限公司 A kind of seed soaking agent and preparation method thereof for vegetable seeds
CN108703161A (en) * 2018-07-18 2018-10-26 安徽省金海种业有限公司 A kind of paddy rice seed soaking agent
CN109362813A (en) * 2018-11-05 2019-02-22 贵州好菇粮农业科技有限公司 A kind of mushroom growth regulator and preparation method thereof
CN114009174A (en) * 2021-12-02 2022-02-08 广东石油化工学院 Method for promoting germination rate of pepper seeds
CN114982415A (en) * 2022-05-26 2022-09-02 黑龙江省林业科学研究所 Method for improving germination rate of alpine rhododendron and seedling raising method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
朱立成 ; 王祥胜 ; 刘文 ; 张明浪 ; 刘信忠 ; .牡丹皮等16种中草药提取物抑制植物病原菌的研究.植物保护.2007,(03),第83-86页. *

Also Published As

Publication number Publication date
CN115517269A (en) 2022-12-27

Similar Documents

Publication Publication Date Title
CN105052655B (en) Oil tea implantation methods
CN106797861A (en) A kind of oil tea implantation methods
CN1922987B (en) Multiple-ear rock lithocarpus glaber dwarfing rapid reproduction method
CN103907472A (en) Method for cultivating caesalpinia sappan linn in karst region
CN102630562A (en) Tissue culture and rapid propagation method of Jiuhua Polygonatum sibiricum
CN107333612A (en) A kind of implantation methods of evodia rutaecarpa
CN103651122A (en) Bletilla striata protocorm induction medium
CN106912341A (en) A kind of implantation methods of high mountain tea tree
CN106465588A (en) A kind of method for culturing seedlings improving Rhododendron seeds germination percentage
CN102090256A (en) Method for easily cutting IlexXmeserveae S.Y.Hu. 'Blue Girl' by using magan soil
CN100586268C (en) Method for breeding saw palmetto
CN104969780B (en) Hei Beila engrafting method
CN106234149A (en) A kind of hybrid breeding method of dragon hat Fructus Pruni pseudocerasi
CN113785706A (en) Cutting seedling method of Siberian apricot trees
CN101223847B (en) Culture method of Myrobalan seedlings
CN115517269B (en) Rhododendron seedling raising method
CN110583408A (en) High-yield millettia speciosa champ planting method
CN106342516A (en) Seedling raising method for improving survival rate of Rhododendron
CN116158268A (en) Cutting method for tea tree short-spike nursery
CN107455211A (en) A kind of implantation methods of Chinese yew interplanting sorghum
CN114600891A (en) Composition and method for seed germination
CN107637291A (en) A kind of tea shoot breeding method
CN113711787B (en) Method for whole-branch cuttage rapid breeding of Lumai camellia nitidissima
CN106718086A (en) A kind of grafting seedlings-growing method of abortion orange osmanthus
CN112673826B (en) Seedling breeding method by utilizing radix codonopsis pilosulae

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant