CN115515612A - 用于抑制致病性大肠杆菌增殖的组合物和方法 - Google Patents

Abstract

一种用于预防或治疗由致病性大肠杆菌引起的感染或疾病的组合物，包括具有裂解致病性大肠杆菌的能力的肌尾噬菌体科噬菌体和药学上可接受的载体。一种用于预防或治疗由致病性大肠杆菌引起的感染或疾病的方法，包括向受试者给药肌尾噬菌体科噬菌体以及用肌尾噬菌体科噬菌体裂解致病性大肠杆菌。

Description

用于抑制致病性大肠杆菌增殖的组合物和方法

本申请要求于2020年4月4日提交的美国非临时专利申请号16/840,334的优先权，该申请出于所有目的以引用方式并入，如同在本文中完整阐述一样。

技术领域

本发明涉及用于抑制致病性大肠杆菌(Escherichia coli)增殖的组合物和方法，更具体地说，涉及含肌尾噬菌体科噬菌体(Myoviridae bacteriophage)的组合物及其使用方法。

背景技术

大肠杆菌是一种革兰氏阴性、兼性厌氧、杆状大肠杆菌属大肠菌型细菌。在血清学上，根据其是否含有体细胞(O)、鞭毛(H)或荚膜(K)抗原进行细分，已知这些抗原与大肠杆菌的致病性相关。致病性大肠杆菌是指获得了少量的能够在大肠杆菌中表达的毒力因子的大肠杆菌，根据其发病特征和毒素种类，有五种致病性大肠杆菌，即肠出血性大肠杆菌、产肠毒素大肠杆菌、肠侵袭性大肠杆菌、肠致病性大肠杆菌和肠聚集性大肠杆菌。

致病性大肠杆菌可引起多种疾病，如食物中毒、急性胰腺炎、尿路感染、败血症和癌症。在致病性大肠杆菌相关的癌症中，结直肠癌是最常见的癌症之一，约占所有癌症病例的10％和所有癌症死亡的8％。此外，结直肠癌在全球非常常见，并通过促使正常粘膜向腺癌的转变的结肠上皮细胞突变的累积而发展。作为导致结直肠癌发生的主要原因之一，结肠息肉是指结肠粘膜异常生长并成为突出到肠内的疣状肿块的情况。它通常分为可能发展成癌症的肿瘤性息肉和不太可能发展成癌症的非肿瘤性息肉。在各种类型的息肉中，腺瘤性息肉随着时间的推移更有可能发展为癌症。虽然致病性大肠杆菌引起的腹泻是一种值得注意的疾病，但一些致病性大肠杆菌的定殖与促使腺瘤性息肉的形成而促使结直肠癌的发展有关。

通常，疫苗和抗生素被用于致病性大肠杆菌的传染性疾病的预防和治疗。在这里，由于抗生素耐药性致病性大肠杆菌的增加，抗生素的有效性一直在不断下降，需要研发除目前处方抗生素以外的有效方法。

最近，使用噬菌体作为防治细菌性传染性疾病的对策引起了广泛关注。噬菌体是感染细菌的非常小的微生物，通常简称为“噬菌体”。一旦噬菌体感染细菌细胞，噬菌体就会在细菌细胞内增殖。增殖后，噬菌体的后代破坏细菌细胞壁并从宿主细菌中逃脱，表明噬菌体具有杀灭细菌的能力。噬菌体感染细菌的方式的特点是其非常高的特异性，因此感染特定细菌的噬菌体类型的数量是有限的。也就是说，某种噬菌体只能感染特定的细菌，这表明某种噬菌体仅能杀灭特定的细菌而不能伤害其他细菌。由于噬菌体的这种细菌特异性，噬菌体仅对目标细菌具有抗菌作用，但不影响包括人类在内的动物中的共生细菌。广泛用于细菌治疗的传统抗生素附带地也会影响多种细菌。这会导致诸如正常菌群紊乱的问题。另一方面，噬菌体的使用不会干扰正常菌群，因为目标细菌被选择性地杀灭。因此，可以安全地使用噬菌体，与任何其他抗生素相比，这大大降低了使用中发生不良反应的可能性。

由于噬菌体具有杀灭细菌的独特的能力，自发现以来，噬菌体作为一种防治细菌感染的潜在的有效对策引起了人们的关注，并进行了大量相关研究。

噬菌体往往对细菌具有高度特异性。由于这种特异性，噬菌体通常仅对某些细菌菌株具有抗菌作用，即使这些细菌属于同一菌种。此外，噬菌体的抗菌强度可能取决于目标菌株的类型。因此，有必要收集多种有用的噬菌体，以便有效控制特定细菌。因此，为了研发针对致病性大肠杆菌的有效噬菌体利用方法，必须获得对致病性大肠杆菌具有抗菌作用的多种噬菌体。此外，需要筛选产生的噬菌体，以确定它们在抗菌强度和抗菌谱方面是否优于其他噬菌体。

发明内容

所要解决的技术问题

因此，本发明考虑到相关技术中遇到的问题并且旨在解决这些问题。

在一个实施方式中，用于预防或治疗由致病性大肠杆菌引起的感染或疾病的组合物包括：具有裂解致病性大肠杆菌的能力的肌尾噬菌体科噬菌体以及药学上可接受的载体。

在另一个实施方式中，肌尾噬菌体科噬菌体包括如SEQ ID NO:1所述的序列。

在另一个实施方式中，肌尾噬菌体科噬菌体的浓度为1×10¹pfu/ml至1×10³⁰pfu/ml或1×10¹pfu/g至1×10³⁰pfu/g。

在另一个实施方式中，肌尾噬菌体科噬菌体的浓度为1×10⁴pfu/ml至1×10¹⁵pfu/ml或1×10⁴pfu/g至1×10¹⁵pfu/g。

在另一个实施方式中，药学上可接受的载体是乳糖、葡萄糖、蔗糖、山梨醇、甘露醇、淀粉、阿拉伯胶、磷酸钙、海藻酸盐、明胶、硅酸钙、微晶纤维素、聚乙烯吡咯烷酮、纤维素、水、糖浆、甲基纤维素、甲基羟基苯甲酸酯(methylhydroxybenzoate)、丙基羟基苯甲酸酯(propylhydroxybenzoate)、滑石粉、硬脂酸镁或矿物油。

在另一个实施方式中，组合物还包括选自由润滑剂、润湿剂、甜味剂、香味剂、乳化剂、悬浮剂和防腐剂组成的组的一个或多个。

在另一个实施方式中，致病性大肠杆菌是肠出血性大肠杆菌、产肠毒素大肠杆菌、肠侵袭性大肠杆菌、肠致病性大肠杆菌、肠聚集性大肠杆菌或致癌性大肠杆菌。

在另一个实施方式中，感染或疾病是食物中毒、胃肠炎、腹泻、尿路感染、新生儿脑膜炎、溶血性尿毒症综合征、腹膜炎、乳腺炎、败血症、革兰氏阴性肺炎、志贺氏菌病、痢疾或癌症。

在另一个实施方式中，组合物是溶液、悬浮液、油乳剂、水溶性介质、提取物、粉末、颗粒、片剂或胶囊。

在另一个实施方式中，组合物进一步包括具有裂解致病性大肠杆菌或非大肠杆菌细菌种的能力的第二噬菌体。

在另一个实施方式中，肌尾噬菌体科噬菌体具有20-70分钟的潜伏期和80-200PFU/感染细胞的裂解量。

在另一个实施方式中，潜伏期为35-40分钟，裂解量为110-120PFU/感染细胞。

在一个实施方式中，用于预防或治疗由致病性大肠杆菌引起的感染或疾病的方法包括向受试者给药肌尾噬菌体科噬菌体；以及用肌尾噬菌体科噬菌体裂解致病性大肠杆菌。

在另一个实施方式中，肌尾噬菌体科噬菌体包括如SEQ ID NO:1所述的序列。

在另一个实施方式中，肌尾噬菌体科噬菌体的浓度为1×10¹pfu/ml至1×10³⁰pfu/ml或1×10¹pfu/g至1×10³⁰pfu/g。

在另一个实施方式中，肌尾噬菌体科噬菌体的浓度为1×10⁴pfu/ml至1×10¹⁵pfu/ml或1×10⁴pfu/g至1×10¹⁵pfu/g。

应当理解，上述一般描述和以下详细描述都是示例性和解释性的，旨在提供对所要求保护的本发明的进一步解释。

发明的有益效果

与基于抗生素的传统组合物和方法相比，本申请的用于抑制致病性大肠杆菌增殖的组合物和方法对致病性大肠杆菌具有高特异性。该组合物可用于预防或治疗致病性大肠杆菌感染，而不影响其他有用的共生菌，并且具有较小的副作用。一般来说，当使用抗生素时，共生菌也受到损害，因此由于其使用而产生各种副作用。同时，在噬菌体对相同细菌菌种表现出抗菌活性的情况下，噬菌体的每种抗菌特性(例如抗菌强度和抗菌谱(宿主范围))是不同的，并且噬菌体通常仅对相同细菌菌种中的某些细菌菌株有效。因此，本申请的组合物和方法在其工业应用中提供了不同的效果。

附图说明

附图是为了提供对本发明的进一步理解，被纳入本说明书并构成本说明书的一部分，附图说明了本发明的实施方式，并与描述一起用于解释本发明的原理。

在附图中：

图1是示出噬菌体Esc-COP-15的形态的电子显微照片。

图2是噬菌体Esc-COP-15的主要结构蛋白的分析结果。

图3是示出噬菌体Esc-COP-15杀灭大肠杆菌能力的实验结果的照片。透明区是由目标细菌裂解形成的斑块。

图4是噬菌体Esc-COP-15的一步生长曲线。

具体实施方式

现在将详细参考本发明的实施方式，其示例在附图中示出。

根据本发明的一个方面，本发明提供了一种名为Esc-COP-15的肌尾噬菌体科噬菌体，其具有特异性杀灭大肠杆菌的能力，并具有包含如SEQ ID NO:1所述序列的基因组。本发明还提供了使用包含其作为活性成分的组合物用于预防和治疗由致病性大肠杆菌引起的感染或疾病的方法。

噬菌体Esc-COP-15由本申请的发明人分离，然后于2019年11月15日保藏在韩国生命工学研究院(Korea Research Institute of Bioscience and Biotechnology)的韩国典型培养物保藏中心(Korea Collection for Type Cultures)(保藏号：KCTC 14027BP)。

噬菌体Esc-COP-15的潜伏期和裂解量分别为20-70分钟和80-200PFU/感染细胞，优选分别为35-40分钟和110-120PFU/感染细胞，但不限于此。

此外，本发明提供了一种可用于预防或治疗由致病性大肠杆菌引起的感染或疾病的组合物，其包括噬菌体Esc-COP-15作为活性成分。

因为包含在本发明组合物中的噬菌体Esc-COP-15能有效杀灭致病性大肠杆菌，所以认为其在预防致病性大肠杆菌感染或治疗由致病性大肠杆菌引起的疾病方面有效。因此，本发明的组合物能够被用于预防和治疗由致病性大肠杆菌引起的疾病。

本发明中由致病性大肠杆菌引起的疾病包括食物中毒、胃肠炎、腹泻、尿路感染、新生儿脑膜炎、溶血性尿毒症综合征、腹膜炎、乳腺炎、败血症、革兰氏阴性肺炎、志贺氏菌病、痢疾和癌症，但不限于此。

本发明组合物中包含的药学上可接受的载体是通常用于制备药物制剂的载体，其示例包括乳糖、葡萄糖、蔗糖、山梨醇、甘露醇、淀粉、阿拉伯胶、磷酸钙、海藻酸盐、明胶、硅酸钙、微晶纤维素、聚乙烯吡咯烷酮、纤维素、水、糖浆、甲基纤维素、甲基羟基苯甲酸酯(methylhydroxybenzoate)、丙基羟基苯甲酸酯(propylhydroxybenzoate)、滑石粉、硬脂酸镁和矿物油，但不限于此。除了上述成分之外，本发明的组合物还可以包括润滑剂、润湿剂、甜味剂、香味剂、乳化剂、悬浮剂和防腐剂。

在本发明的组合物中，包括噬菌体Esc-COP-15作为活性成分。包括的噬菌体Esc-COP-15的浓度为1×10¹pfu/ml至1×10³⁰pfu/ml或1×10¹pfu/g至1×10³⁰pfu/g，优选浓度为1×10⁴pfu/ml至1×10¹⁵pfu/ml或1×10⁴pfu/g至1×10¹⁵pfu/g。

本发明的组合物可以根据本发明所属领域的普通技术人员使用单位剂量或多剂量容器形式的药学上可接受的载体和/或赋形剂来容易地进行的方法进行配置。然后，制剂可以是溶液、悬浮液或油乳剂或水溶性介质、提取物、粉末、颗粒、片剂或胶囊的形式。可以另外包括分散剂或稳定剂。

为了提高上述目的的有效性，可在本发明组合物中进一步包括对非大肠杆菌细菌菌种具有抗菌活性的噬菌体。此外，在本发明的组合物中还可以包括对大肠杆菌具有抗菌活性的其他种类的噬菌体。可以附加地包括这些噬菌体，以使抗菌效果最大化，因为噬菌体的每种抗菌性质(例如抗菌强度和抗菌谱(宿主范围))在噬菌体对相同细菌菌种表现出抗菌活性的情况下是不同的。

在本说明书中，术语“预防(prevention)”和“预防(prevent)”表示(i)阻断致病性大肠杆菌感染；和(ii)抑制由致病性大肠杆菌感染引起的疾病的进展。

在本说明书中，术语“治疗(treatment)”和“治疗(treat)”表示所有以下活动：(i)抑制由致病性大肠杆菌引起的疾病；和(ii)减轻由致病性大肠杆菌引起的疾病的病理状况。

在本说明书中，术语“致病性大肠杆菌”表示肠出血性大肠杆菌、产肠毒素大肠杆菌、肠侵袭性大肠杆菌，肠致病性大肠菌、肠聚集性大肠杆菌和致癌性大肠杆菌，但不限于此。

在本说明书中，术语“由致病性大肠杆菌引起的疾病”和“致病性大肠菌感染”表示食物中毒、胃肠炎、腹泻、尿路感染、新生儿脑膜炎、溶血性尿毒症综合征、腹膜炎、乳腺炎、败血症、革兰氏阴性肺炎、志贺氏菌病、痢疾和癌症，但不限于此。

在本说明书中，术语“潜伏期”表示噬菌体微粒在受感染的宿主细胞内繁殖所需的时间。

在本说明书中，术语“裂解量”表示每个被感染的细菌产生的噬菌体的数量。

在本说明书中，术语“分离(isolate)”、“分离(isolating)”和“分离(isolated)”表示通过应用各种实验技术从自然界中分离噬菌体的行为，以及确保能够区分目标噬菌体和其他噬菌体的特性的行为，并且还包括使用生物工程技术增殖目标噬菌体从而使得目标噬菌体可工业应用的行为。

如以下示例所示，本发明的实际的和当前优选的实施方式是说明性的。

然而，应当理解，本领域技术人员在考虑本公开内容的基础上，可以在本发明的精神和范围内进行修改和改进。

实施例1：能够杀灭大肠杆菌的噬菌体的分离

从环境或临床样本中收集样本，以分离能够杀灭大肠杆菌的噬菌体。这里，用于噬菌体分离的大肠杆菌菌株已经由本申请的发明人预先分离并鉴定为大肠杆菌。

下文详细描述了分离噬菌体的步骤。将收集的样本添加到以1/1000的比例接种大肠杆菌的TSB(胰酶大豆肉汤)培养基(酪蛋白消化物17g/L；大豆消化物3g/L；葡萄糖2.5g/L；氯化钠5g/L；磷酸氢二钾2.5g/L)，然后在37℃振荡培养3-4小时。培养完成后，以8,000rpm离心20分钟，回收上清液。回收的上清液以1/1000的比例接种大肠杆菌，然后在37℃振荡培养3-4小时。当样本含有噬菌体时，为了充分增加噬菌体的数量(滴度)，将上述步骤重复总共5次。重复该步骤5次后，将培养液以8,000rpm离心20分钟。离心后，使用0.45μm过滤器过滤回收的上清液。将获得的滤液用于典型的斑点试验，以检查其中是否包含能够杀灭大肠杆菌的噬菌体。

斑点试验如下进行：以1/1000的比例将大肠杆菌接种到TSB培养基，然后在37℃下振荡培养过夜。将2ml(OD₆₀₀为1.5)的上述制备的大肠杆菌培养液涂抹在TSA(酪蛋白消化物15g/L；大豆消化物5g/L，氯化钠5g/L；琼脂15g/L)平板上。将平板放置在清洁的工作台上约30分钟以干燥涂抹的溶液。干燥后，将10μl制备好的滤液点在其上涂有大肠杆菌的平板培养基上，然后放置干燥约30分钟。干燥后，将被点滴的平板培养基在37℃培养一天，然后检查在滴有滤液的位置透明区的形成。在滤液产生透明区的情况下，则判断其中包括能够杀灭大肠杆菌的噬菌体。通过上述检查，可以获得含有具有杀灭大肠杆菌能力的噬菌体的滤液。

从上述确认的滤液中分离出纯噬菌体，以得到能够杀灭大肠杆菌的噬菌体。传统的噬菌斑测定用于分离纯噬菌体。详细地说，使用灭菌的尖端回收在噬菌斑测定过程中形成的斑块，然后将其添加到大肠杆菌的培养溶液中，然后在37℃培养4-5小时。培养后，以8,000rpm离心20分钟以获得上清液。将大肠杆菌培养液以1/50的体积比添加到获得的上清液中，然后在37℃培养4-5小时。为了增加噬菌体的数量，重复上述步骤至少5次。然后，以8,000rpm离心20分钟，以获得最终上清液。使用得到的上清液进一步进行噬菌斑测定。一般来说，纯噬菌体的分离不是通过步骤的单次迭代完成的，因此使用上述得到的斑块重复上述步骤。在至少5次重复该步骤后，获得含有纯噬菌体的溶液。通常重复分离纯噬菌体的步骤，直到生成的斑块在大小和形态上变得彼此相似。此外，使用电子显微镜确认纯噬菌体的最终分离。重复上述步骤，直到使用电子显微镜确认分离出纯噬菌体。根据常规方法操作电子显微镜。简言之，将含有纯噬菌体的溶液加载到铜网格上，然后用2％醋酸铀酰进行负染色并干燥。然后使用透射式电子显微镜观察其形态。分离的纯噬菌体的电子显微照片如图1所示。根据形态学特征，确认上述分离的新噬菌体属于肌尾噬菌体科噬菌体。

对含有上述确认的纯噬菌体的溶液进行以下纯化过程。以噬菌体溶液的总体积计，以1/50的体积比将大肠杆菌培养液添加到含有纯噬菌体的溶液中，然后进一步培养4-5小时。培养后，以8,000rpm离心20分钟以获得上清液。重复该步骤总共5次，以获得含有足够数量的噬菌体的溶液。将最终离心得到的上清液使用0.45μm过滤器过滤，然后采用常规聚乙二醇(PEG)沉淀工艺。具体地，将PEG和NaCl添加到100ml滤液中，直到达到10％ PEG8000/0.5M NaCl，然后在4℃下放置2-3小时。此后，以8,000rpm离心30分钟以获得噬菌体沉淀。将所得噬菌体沉淀悬浮于5ml缓冲液中(10mM Tris-HCl、10mM MgSO₄、0.1％明胶、pH8.0)。所得材料称为噬菌体悬浮液或噬菌体溶液。

因此，收集上述纯化的纯噬菌体，命名为噬菌体Esc-COP-15，然后于2019年11月15日保藏在韩国生命工学研究院(Korea Research Institute of Bioscience andBiotechnology)的韩国典型培养物保藏中心(Korea Collection for Type Culture)(保藏号：KCTC 14027BP)。

实施例2：噬菌体Esc-COP-15基因组的分离和序列分析

噬菌体Esc-COP-15的基因组如下进行分离。从使用与实施例1相同的方法获得的噬菌体悬浮液中分离基因组。首先，为了去除悬浮液中包含的大肠杆菌的DNA和RNA，将200U的脱氧核糖核酸酶I(DNase I)和核糖核酸酶A(RNase A)分别添加到10ml噬菌体悬浮溶液中，然后在37℃放置30分钟。静置30分钟后，为了停止脱氧核糖核酸酶I(DNase I)和核糖核酸酶A(RNase A)的活性，向其中加入500μl的0.5M乙二胺四乙酸(EDTA)，然后静置10分钟。此外，将所得混合物进一步在65℃放置10分钟，然后向其中加入100μl蛋白酶K(20mg/ml)以破坏噬菌体的外壁，随后在37℃反应20分钟。之后，向其中加入500μl 10％十二烷基硫酸钠(SDS)，然后在65℃反应1小时。反应1小时后，向反应溶液中加入10ml以25:24:1的组分比混合的苯酚∶氯仿∶异戊醇溶液，然后彻底混合。此外，将所得混合物以13,000rpm离心15分钟以分离成多层。在分离的层中，选择上层，并以1.5的体积比向其中加入异丙醇，随后以13,000rpm离心10分钟以沉淀基因组。收集沉淀物后，向沉淀物中加入70％乙醇，然后以13,000rpm离心10分钟以洗涤沉淀物。回收洗涤的沉淀物，真空干燥，然后溶于100μl水中。重复该过程以获得足够量的噬菌体Esc-COP-15的基因组。

通过使用来自首尔国立大学环境管理国家仪器中心(National InstrumentationCenter for Environmental Management,Seoul National University)的Illumina Mi-Seq设备进行下一代测序分析，获得了上述获得的噬菌体Esc-COP-15基因组序列信息。最终分析的噬菌体Esc-COP-15的基因组大小为152,531bp，全基因组序列由SEQ ID NO:1表达。

使用BLAST调查对上述获得的噬菌体Esc-COP-15基因组序列与先前报道的噬菌体基因组序列的同源性(相似性)进行了研究，发现噬菌体Esc-COP-15的基因组序列与大肠杆菌噬菌体ESCO13(基因库登录号KX552041.2)的序列具有相对高的同源性(同一性：79％)。然而，噬菌体Esc-COP-15基因组上的开放阅读框(open reading frame)(ORF)数量为292，而大肠杆菌噬菌体ESCO13具有282个开放阅读框，与噬菌体Esc-COP-9不同。

根据这一结果，可以得出结论，噬菌体Esc-COP-15一定是一种不同于传统报道的噬菌体的新型噬菌体。此外，由于噬菌体的抗菌强度和抗菌谱通常取决于噬菌体的类型，因此认为噬菌体Esc-COP-15可以提供不同于先前报道的任何其他噬菌体的抗菌活性。

实施例3：噬菌体Esc-COP-15的主要结构蛋白的分析

进行一维电泳以分析噬菌体Esc-COP-15的主要结构蛋白。为了获得组成噬菌体Esc-COP-15外壁的蛋白质，将200μl的实施例1中制备的噬菌体悬浮液与800μl丙酮混合，并剧烈旋转。混合物在-20℃下静置10分钟。以13,000rpm在4℃离心20分钟以除去上清液，然后空气干燥。将沉淀物重新悬浮在50μl电泳样本缓冲液(5×)中，然后将其煮沸5分钟。通过一维电泳分析制备的样本。因此，如图2所示，确认了大小约为13kDa、35kDa、100kDa和157kDa的主要结构蛋白。

实施例4：噬菌体Esc-COP-15杀灭致病性大肠杆菌能力的研究

研究了噬菌体Esc-COP-15杀灭致病性大肠杆菌的能力。为了研究杀灭能力，以与实施例1中所述相同的方式使用斑点试验观察透明区的形成。将总共6株已鉴定为pks阳性的大肠杆菌菌株(其为pks基因组岛的阳性载体)用作致病性大肠杆菌以研究杀灭能力。噬菌体Esc-COP-15具有裂解和杀灭作为实验目标的6株致病性大肠杆菌中总共5株的能力。其实验结果示于表1中，代表性结果示于图3中。

[表1]噬菌体Esc-COP-15的抗菌活性测试

同时，也在单独的实验中研究了噬菌体Esc-COP-15对支气管败血症博代氏杆菌(Bordetella bronchiseptica)、粪肠球菌(Enterococcus faecalis)、屎肠球菌(Enterococcus faecium)、缓症链球菌(Streptococcus mitis)、乳房链球菌(Streptococcus uberis)和铜绿假单胞菌(Pseudomonas aeruginosa)的杀灭能力。结果，噬菌体Esc-COP-15不具有杀灭这些细菌的能力。

因此，证实噬菌体Esc-COP-15具有较强的杀灭致病性大肠杆菌的能力和针对致病性大肠杆菌的广谱抗菌谱，这表明噬菌体Esc-COP-15可以用作用于预防和治疗致病性大肠杆菌感染的组合物的活性成分。

实施例5：噬菌体Esc-COP-15的生长特性

噬菌体Esc-COP-15的生长特性通过一步生长曲线分析进行分析。噬菌体Esc-COP-15的一步生长曲线分析如下进行：将50ml TSB(胰酶大豆肉汤，Difco)培养基以1/1000的比例接种大肠杆菌，然后振荡培养直至指数期(OD₆₀₀＝0.3～0.4)。培养完成后，以8,000rpm离心5分钟，回收细菌细胞沉淀物。将回收的沉淀物悬浮在50ml TSB中。所得材料可称为细菌悬浮液。将噬菌体Esc-COP-15与细菌悬浮液以0.1的感染复数(MOI)混合，并在室温下培养10分钟，然后以12,000rpm离心30秒。除去上清液后，将含有噬菌体感染的细菌细胞的沉淀物悬浮于50ml TSB中，并在37℃振荡培养。在60分钟内以5分钟间隔取等分试样，并立即通过常规噬菌斑测定法测定等分试样中的滴度(图4)。

噬菌体Esc-COP-15的潜伏期估计约为35±5分钟，平均裂解量约为110±10pfu/感染细胞。

实施例6：关于使用噬菌体Esc-COP-15预防致病性大肠杆菌感染的实验实施例

将100μl噬菌体Esc-COP-15悬浮液(1×10⁸pfu/ml)加入含有9ml TSB培养基的试管中。在含有9ml TSB培养基的另一试管中，仅进一步添加相同量的TSB培养基。然后将致病性大肠杆菌(pks阳性菌株CCARM 1G930)培养液添加到每个试管中，使得在600nm处的吸光度达到约0.5。添加致病性大肠杆菌后，将试管转移到37℃的培养箱中，然后进行振荡培养，在此期间观察致病性大肠杆菌的生长。如表2所示，观察到在添加了噬菌体Esc-COP-15悬浮液的试管中抑制了致病性大肠杆菌的生长，而在未添加噬菌体悬浮液的试管中没有抑制致病性大肠杆菌的生长。

[表2]噬菌体Esc-COP-15的细菌生长抑制试验

上述结果表明，本发明的噬菌体Esc-COP-15不仅抑制致病性大肠杆菌的生长，而且具有杀灭致病性大肠杆菌的能力。因此，得出结论，噬菌体Esc-COP-15可用作用于预防致病性大肠杆菌感染的组合物的活性成分。

实施例7：噬菌体Esc-COP-15对动物模型中大肠杆菌感染的预防作用

研究了噬菌体Esc-COP-15对受大肠杆菌影响的断奶仔猪的预防作用。将4头25日龄断奶仔猪组在一起；每个猪圈(1.1m×1.0m)共饲养2组猪。提供加热系统，并控制周围环境。始终控制猪圈的温度和湿度，每天清洁地板。从实验的第1天开始，按照常规饲料供应步骤向实验组的猪(添加噬菌体)喂食添加了1×10⁸pfu/g的噬菌体Esc-COP-15的饲料，同时按照常规步骤向对照组的猪(不添加噬菌体)喂食不添加噬菌体Esc-COP-15的相同的饲料。从实验第7天开始，两组的饲料均被1×10⁸cfu/g致病性大肠杆菌污染2天，然后分别为实验组和对照组每天提供两次，以引起致病性大肠杆菌感染。给药的致病性大肠杆菌悬浮液制备如下：使用TSB培养基在37℃培养致病性大肠杆菌(菌株CCARM 1G931)18小时，之后分离细菌并使用生理盐水(pH 7.2)将细菌调节至10⁹CFU/ml。从提供受污染饲料2天后的第二天(实验的第9天)开始，实验组(添加噬菌体)的猪按照之前的常规饲料供应步骤再次喂食添加了1×10⁸pfu/g噬菌体Esc-COP-15且不污染致病性大肠杆菌的饲料，而对照组(不添加噬菌体)的猪按照常规步骤喂食不添加噬菌体的相同饲料。从实验第9天开始，每天检查所有试验动物的腹泻情况。通过根据腹泻指数进行测量来确定腹泻程度。腹泻指数采用常用的粪便稠度(FC)评分来测量(正常：0，软便：1，轻度腹泻(loose diarrhea)：2，严重腹泻：3)。结果如表3所示。

[表3]粪便稠度评分

从上述结果可以证实，本发明的噬菌体Esc-COP-15可以非常有效地抑制致病性大肠杆菌的感染。

实施例8：使用噬菌体Esc-COP-15治疗致病性大肠杆菌的传染性疾病的实施例

噬菌体Esc-COP-15对致病性大肠杆菌引起的疾病的治疗效果评估如下：将40只8周龄小鼠分为两组，每组20只小鼠，然后将每组5只小鼠的亚组分别饲养在单独的实验鼠笼中，实验进行7天。在实验的第二天，通过腹腔注射向所有小鼠给药0.1ml致病性大肠杆菌悬浮液。给药的致病性大肠杆菌悬浮液制备如下：使用TSB培养基在37℃培养致病性大肠杆菌(菌株CCARM 1G931)18小时，之后分离细菌并使用生理盐水(pH 7.2)将细菌调节至10⁹CFU/ml。在给药致病性大肠杆菌后2小时，通过腹腔注射给实验组小鼠(用噬菌体悬浮液给药)给药10⁹pfu的噬菌体Esc-COP-15。通过腹膜内注射给对照组小鼠(不给药噬菌体悬浮液)给药0.1ml盐水。对照组和实验组均同等喂食饲料和饮用水。从给药致病性大肠杆菌开始直到试验结束，每天观察小鼠是否存活。结果如下表4所示。

[表4]存活率

从上述结果可以明显地得出结论，本发明的噬菌体Esc-COP-15在治疗致病性大肠杆菌引起的疾病方面非常有效。

本领域技术人员将认识到，上述描述中公开的概念和具体实施方式可以容易地用作改进或设计其他实施方式以实现本发明的相同目的的基础。本领域技术人员还将理解，这样的等效实施方式不脱离所附权利要求中所述的本发明的精神和范围。

本领域技术人员将清楚，在不脱离本发明的精神或范围的情况下，可以对本发明进行各种改进和变化。因此，本发明旨在覆盖本发明的改进和变化，只要它们在所附权利要求及其等价物的范围内。

保藏号

保藏机构名称：KCTC

保藏号：KCTC 14027BP

保藏日期：20191115

序列表

<110> 尹特荣生物科技株式会社

<120> 用于抑制致病性大肠杆菌增殖的组合物和方法

<130> 200010054WO

<150> US 16/840,334

<151> 2020-04-04

<160> 1

<170> PatentIn版本 3.5

<210> 1

<211> 152531

<212> DNA

<213> 肌尾噬菌体科噬菌体(Myoviridae bacteriophage)

<400> 1

agcacgttct cttaaacgta aataaaatca gaaaaatctt gatttgttgg tcgatttgtt 60

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gcacctttac accgtagtaa tcatatgttt aggttgttaa gtgattgatt gataaaggat 180

tgtttgaaaa gtgatggtta agttaggccc gaccttacag cagggcaaaa catcgcctaa 240

gttattgaat acgaatgata atgataatta ttctcattga tatttgagga ttccggctcg 300

cagaagtgga ggggccgaaa aattcgcgtt cagcactcct gacgtatgcg ccataaatcc 360

ttgctaccaa aggcttttct tctcttgccg aagagatatt cttgccgata aatcgcagga 420

acaaggggag gttcaaagaa ccgtccccgt agatattttg ccgagtaata tttgaccaaa 480

gggggtgtca aaaataacag gtatatatag aataattgat agggaatacc aatgataaat 540

gaattgtaat tgccgaggat tgatcgtatg atgttcctcg ttgagagtta cgatgtttgt 600

catgaaacca agagaggaga ttatcatgca agagagtaaa aatgttccgt tgagtagtga 660

tacacttctg ctgttctata agaattggat ggctcaccgt aatgagttgg cgaacacagc 720

gaatggcttg tgcgtatgtt taggcaattt tgcatgggaa gtattgtatc cgaagcatgg 780

tgcagatgtg gatcagttct gggagatgcg tgaagcttta ggtgatgaaa tgatgataca 840

gtttatctat gcagggttgt ctaaaagcac acctttcaat actaacttct tcgaatacga 900

ggaagaatgt gaaaacagaa aatgtcatct caaccaacta cgtgtgcaat gggtgaaaga 960

ccggattcag gattgtgaag gaggtaaata tgaaaagtaa tattccgttg agcagtgcaa 1020

cattgcttcg cttctacaaa gcatggatgg caaaccatcc tgaaatggat cgcacgacga 1080

atggactgtg taattgtctg gaagcatatg tgggaagtta caatccttca actgaaagat 1140

ggcgtgccaa catgttgggc agtctgaaat atgaaatgag gaaacagttt gaatgtgcaa 1200

aactggacta caattttccg ttcaactgtg gttatgggga ttatgcatta gagtgtgacg 1260

ctaataagag tcatgaaaac ccacgccgta ttcagtgggt gcaagatcgc attgaagatg 1320

caaataatgc ttgcattgaa taaaaatgtc tgtatagtgg tttgcatacc gcgcataaca 1380

gatagccaat gaggagattg aaatgtcaaa tatgaaaact gtatccttgt atttagctaa 1440

cacggatcgt gtgcacaccc ttgctaatgc aagtcgtgta ctcgtgcagt tccgaaactt 1500

tgcaccgtcc gttgtgacag gtagtgaaac ctgcgttatc gatttcatta atgagtattt 1560

tcaaggtaat cccgcaggtc gccattttgt taagactcta aaggatgcgg agcaggaaca 1620

atgattcttc cagtagataa tgagatagct atgaatgttc tggatacaga atacttctat 1680

aatcactact ctgagacaga gtggatgaac acgcaaactc tgaaagagaa gacgcaatct 1740

ctgattaagt gtgatcgcta cttgcaacgt gcagaagaag ataatatcat gttagcatct 1800

cgtgcacgac gcgcttccat ctttaataaa atggtagcat tatggggagg tagaaaatga 1860

gcgaaaaacc agtgagagct tccacaatat tggaagcata cctgcaatgt gttgtagatt 1920

acgtcaagag aaactccagc ctactggacg tctaccgtgc agatgacggc gatgttggta 1980

ttgtttatgt gaaacacatt ttatccgaga cacgttatga ggtgttccgt gcaagtatgt 2040

tagggtgtga tcgtgttcgt gtgaactact tggacccgaa ttatcgccat gctcttgaag 2100

atattgaaga ttatcttcct cgctgcccga caggtaatgt aaaagtggca gaagaagcac 2160

cacaaggtgt tgattatcgt gtagccttgt ggaaaaaggc acgtcaacta caagtgctga 2220

atgagatgaa agaggagttg gagcaggaac tgctcaacga cttcgataaa tctccagaag 2280

aggctttagc gtggttgcgt gaagtagaac aataatttaa cataagagga gacactaatg 2340

tttaaaccag gtaatatata tcgtttcaga caaggcactt accaagagtt gtttcaatca 2400

ctctctaaac gattagctga agcgatcggg gagttttggt ttgaagtagt caaaacagac 2460

gcttacggga atgtcctgtc tcttatcatc ttcgtagaag ataagaagct gttctgggat 2520

cagggagtag gcgaggctca aatggatcgc ttccttattg gacacgatca gatcggatat 2580

ttctttgatg gaggcccgtt gttaatggta ggcttaccgc cgctggcacc gcagccaggc 2640

aaggaggttt ccgcgcacga cgatcgtcct gatgcggata ttgtgcctaa aaagtttgca 2700

gttattgttg actacgtggt agtgcatact gtatcatctt tttcagaggc acaagaaaga 2760

gccacgaaag cgaagaaaga gagtccaggg aagcttgtag agatttatac tctgcgtagc 2820

acagcgaaac tcgtaagtaa tgtgatattt gactaagagg agactattat gtttattcaa 2880

gggcattcct ataaattcaa aaatacaaaa gagtataatg cgttccgtga agctgtcaat 2940

ctgaaccgta agatttcaga tctgctgggt ccaaaacact tcctggtgtt ggagacaaca 3000

gaaaaagggg atgtggttaa gttccaaaca tttgaagggc atgtctttga agctaatgca 3060

ggtgctttcg ttgattgtgg tatgctgctg tccaaacatg agttaaagca ttttaacgat 3120

atcacttggt ttgactctta tgttgaggca gaggttggtt tgccaccaga accaaaggaa 3180

gagccagtac aagagccttc tcaagagagt ccagaagata aaaaatatct tgtaatggtg 3240

gatcttgttc ttgtgggcgt tgccggaaat atccgagatg cggaagatct ggcacaatcc 3300

tataaagaag ataacgctaa agccgtggtc gagatttaca atcgctacag cacggcacaa 3360

gttaaagttt attttgaata aatagcttga catagggatc acgttggtag ataatcgttg 3420

catacacaaa tgagaggaga aatgaaatga gcaaaacatt gaaagaattc taccgtgcgt 3480

atgcaacatg gcttgacttt ggcgcgccaa actttaaacc atttagccgc gcgtcaggtt 3540

tgtgcactaa cctttacaac tttagtaaaa atcatgatgt tatcaatgag atgaaatttc 3600

aattcgatgc cgctggactc gacccgaact atcctttcgg tggtgaagat gattacgtag 3660

accgtagtgt cgcacggcag caacatctta accctaagcg tatcaaatgg gtaaaagaac 3720

acactgaata atcaataaga ggagagataa aatgaatttg accgaacagt tgcgcagtat 3780

tgccaaagaa gcttccatcg gttttcctat tgaagaggtt attgagacgt gtttttcaga 3840

agcacgggcg ggaaactacc aacgggattt cacgccctct gattttggct tctgtaatcg 3900

tcctctttcc caggatggga tcgccagaca gcgcgagtta atgaatatgg tggtaacagc 3960

actccgtgag cgtggacttg tggtttatat tgaattagac agaaaccatc aagcttatca 4020

atgcatcact gttgattggt cggaggtgaa ataatgaagg gtaacaagat gaaagttaag 4080

aaagtgccga gcgcatacac tttgtggctt gcgcgagcgt taaaccagac gatcaaagat 4140

cctaagaagc caatgaagag cggaaagaag tttatgtcta aaggcaaaaa aacttttggc 4200

ttcactcgta tggctgttaa gatgaaaagg ctggggtata aaccaaacta tactcgttac 4260

ccttctgaat ataactggtt cgactaaaag gagaaagcaa tggcaggttt aaagttggag 4320

atgttgacaa agcaagaatt tgttcccgaa catggtgaaa cttacattgc tttcttgatc 4380

agtgattttt atgctgattt agaatacgtt gaactggaat gtgagttcca ttaccaaatg 4440

atcgacgcag aaactggcta ttgcctttct acttccgcag tgtataatgg ggataacggt 4500

attcctttta acacagcaat gcgttgggaa ggaaagaatc gcttcaagat cctcacagaa 4560

aaagaaaaag aggaatacaa gcaccacaag gaggaggatc aacgcttctg gtgggagaaa 4620

tcttggttcc tgtccgatcg ccgtaatctc cagatccatc cagaagaact ggatgaaatg 4680

tccatcggga ttatgaagaa gaaagacttc atgttccagg tgagagaagc taataagttc 4740

cgtgttgcaa ctaaagagga gaacaaaaca tgaaaacatc tattcgcgta aaacatgatc 4800

cgaacaccag ttacccatac gcgtatgttg aacgtcatca agggaactat tacaccataa 4860

cacgtaaagt accaactgat acagggttta actttgatgt tattgcactg gatgatcaag 4920

gtgttgataa tctggtgaac gcattaatcg cattgaagga agcatgatat gagaatccga 4980

ctaattgaaa atggggatac catcttcgaa acccgtaaag gggagatcac agagattcca 5040

gaagaaagat caacaatcct tgtggatggt gttttgaaat attgctgtag tgtctacaaa 5100

tcttacgaga aggatcagta tggcgatcca gaaatctggt ttgtaggtga tgtgtattaa 5160

ggagagaaaa tgatcggaca tttctgtaag gtgaaagata tctccctctt tgagggagac 5220

gttgttaagt ttgattgttt caccttggtt tttgaaagat ttttaggtgg agacatggtg 5280

atcttcaagg tagaagggac tgttgacaat tacacagctt acgggctgca ttctgaagtt 5340

tattttcacg gttatgtgaa aagacctctt gtaactaaga tgtgggagga gtatcatata 5400

gacattacag acaaacgtgg tgttttctgg aacgttgaat aagaggagag aacaatggag 5460

agtaagaacg cattcgataa aggctacgca cagggttata aatttggcat gttttgccgc 5520

cacaatcctt acgatcccaa taaacatcct gaaaagtttc aagagtggga agaagggttt 5580

tctcaaggct atcaagataa ggggtggtta tgaaaacatt agaaggatat tacaaggcat 5640

acgcggattg gttagataag ggtgctccgc cttaccgacc tttctcccgt ggtgtagggt 5700

tatgtagcaa tcttcgatat tacactggcg ctgataatat cttgatactt actcgtttag 5760

agcgtcagct tgccgatcag ttcgaagatg cgggactctc tgctgcctat cctttcggag 5820

aagaagcttt tgatttatgt ttggaccaaa acaacatgca ccttgatcct aatcgtattg 5880

cttgggtaag aagccactta gaaaaaggag aaaaccaatg aaaacattag cttactatga 5940

aaacctacgt gatgcttaca tgagcctctc aaatccatcg gtcactgcac aacttttaat 6000

tgaaactctg gaagacctga tcgctcttta tcaggaacct acgaaggatt tgccattgtt 6060

tctcggcgtt cctgtagatt tgccgccagg ttggaggatt ctgatcgatg atattaaggt 6120

tatccggttg attgataact atgatctgat tcgtggggtc attcaccctt gtattcaggg 6180

cggttacaat ataaattggg ataaagaata tctggatcat cgtcaagacc aggcagaggc 6240

tatcaaattt ttgatggaga acattaaacg atgaaaacat tacgagagtt ctatcaagct 6300

tacgcagatt gggtggacgc gggagcgcca gacggcaatc ctttccgccg tgaatggggt 6360

ttatgcacta atctctactt cttcggttat tgtgatgatg atttgctgta tgaaatgtca 6420

aaacagtttg aagctgcggg gttggaccca gaactcccgt tcaataaaga ttgggatgat 6480

tacgcgagag agtataagga agatggttct catctcaatg ctgtccgtat ccaatgggta 6540

agggatcatc tcaaatgatt atagaaatta aactggagta cgccggagcg gtagctttag 6600

ttgcacaaat gcttgacgca aagatcaacg aagtgtatat taatgcttgg agtgtggaga 6660

tagacgtgtc tatggacaat aaagccaacc gtctattggt tgcggaatgt gaactaagag 6720

gtttacttta taaaggagaa ccctcatgaa gattaaagtt atcgatgtta caggctacgt 6780

aggttttgaa aatgttcaac ttccttttat tgttgaagcg caccttttca gcgatgatct 6840

gcaaacattc ttcgtttccc aagaagagat ggctaaagtg gaaggttgtg agatcctgga 6900

ggatgatgtt tgggaaggtt gtgactatgt attcttctct tgggatgtag aagtgatcga 6960

ggaataagaa atgtcttact ttacaagagt tgtttcgttg tgtaaaaagc aatatgagag 7020

tgattacggg cttttcgatg ttcctctgga cctaacagag tattgttcgt gtgggaataa 7080

gttgaaagaa gctttgattt acgcagaagg gagtgataat cttgttaaag tgaaagtgtg 7140

caggaagtgt cattcgattc ttcctgcttc tatgaataag tgagaatgta atatgataga 7200

aactattatt ttcgttgtcc tgattattat tttaggtttc ttaatgtatg aataggaggt 7260

ttaaatgagt aaagtgaagc actacggtgt aaaccccaac gacgcttggc ttatgtgttc 7320

ggatgatccg acagaaggga catatgtgaa gtatgaagat tatgaaaccc tgtcgaaggc 7380

agtgatcgca ctgttaacag atatccgctc ccgttatcca ggacaagatt tcacttgtcc 7440

acatattcag cttcttgccc ttctggtaga aggtgagaaa gaagtggaag atggcttcaa 7500

gcgtgtagag tatcaaggtg aaactttaga tgtgcctgaa tttgttaatt atattgcaac 7560

agattcaagt ggtgatcgtt atggttacag ccataagcca gagattcctg actggattac 7620

cgcttggcgt acttccgtta gtaccggaga ctattacttt cttggtgtgg gtagtagtaa 7680

agattggcgt ttctctttga cagaggttta atcagatgga agagatattg ccagcagcat 7740

atcgtttgaa tgatgaacgt atttgggagt ggaccgacgt ttcttgtttt taccccgaaa 7800

tagttcagta ctggcgagca aaagggtgtg ttgtagttat caatccttgt ggaccagtag 7860

atgaagattg cctctacttg gatggaaagt ggcatagcta ttgtcgctgg ccttttgagt 7920

ggatgcttaa cgaagatctc ccagagggtt gggagtatcc aaaagcaaag gagagaaaga 7980

atgtcttacg gaaataaaac ccgtttattc catgtagtac gtttcctgga tacaaagaaa 8040

gaataccttc tgcctttcat caccgaaccg gatcaagaag tgtttcgcct tactgaagat 8100

ggtgattttg ccactaccag tttggtaagt tatatgatta acgaagctat tgatgagggg 8160

cgagcaatct ctgtaggttt tgctacgctc tctttgtctg taatttctaa tgaggaggta 8220

gaatgagcaa ggtattcgct gtttactgta gtctctctaa gcattccttt gttaccactg 8280

gtaaaactta cgaggctgaa tgggaggcca aggaaggaga ggtattccga tttacttgtg 8340

acgatgggga ctatgcgtgg gatacattga aagatagtaa actggggcgt tggtctattg 8400

tgggagagaa agaagaacct ttcattcttg ttcctgctga cacttctgtg aactgggaag 8460

tgtctaaagc tgaacaagac caagctaccc ttctccgtag tctggaacat gccgttgaac 8520

aatggaaaga agcacagtgc aactacgata gctctcgtaa acacaaccgt gtgcttgaag 8580

cacgtataga attactggct cttagtttgc gtagccttat tctcgctatt gagcgaggac 8640

tttccaatcc tgatgggttc ccaatcagcg atgtagaaca cgaagtgtat ctggcacggg 8700

gtgcctatta tcatattaaa caagaatctg tagaaggaga gaaatgatgg aagaaaaact 8760

ggaacgtatc atcgatttag ccatgtctat cggctacgat gattgttttc tccagatcct 8820

tggagaatca gagaaagatt acgactcttg ggtgaaagag ttggaagaac tcaaaacttc 8880

cttgattgct gactttgctg aagcagagga taaagcagat cgttacaact atctgtgtcg 8940

gtaaagcttg acagacgctt actgtatggt atgcttacaa catcaaaaca acgaggagag 9000

aaaaatgact aaacttttat gtgtatcatc ttcggttccg tatttcactg ttggtaagca 9060

ataccctgtg acaaatacag acgaggatgt ggactactac tggatcaata ctgatgacgg 9120

aacagattgt gttattgagt tgagaatgcg ctgcgccact tttgaggtgg taactgaaaa 9180

cgtgatgcgt cttttcgaga tccgttatac aaaagataac ggcagtatta aaacttatca 9240

gatcccagcc ctttctatct acgacgcatg tgtacgttta ggtcagattt acggggacga 9300

taaatactac cgtgacgatg tgtccattca tgtggtagag gtggtgtaac atgaataaag 9360

atcggtttct ttctttagtc gcggaagcgc cttttgatgt tcttggggtg agattatgga 9420

aagacagaaa ctatgggtta gatgttcaaa catattctct ttggttggtt ggggagcaca 9480

gcttagatga aagcttgtgc atgggtatgg atactctttc cctctgttta aagtttagaa 9540

gcttagaacg cgcaaacaaa gagcaagaaa aactaacgag gtggctaaat gaaaacaagt 9600

gaagagttga tgaagtttta caaaaagtat cttgcctgga tcgaagatgg tgccccttac 9660

ggtagaccct ttctgccaaa ttgtggtttg tgtgccaact tgttcgatta tgtgcacaga 9720

gacaattaca accccattat aacaaccttg tcagatgaaa tgcacgcgca gttccactat 9780

aaagggttga gtcaagaact ccctttcaat gagaactatg atgcatatgt tttagagtca 9840

gatgcagatg cctgtcacct gaacgtggac aggattaatt gggtgaaaga tcgtattgca 9900

gaggagaatg aatagtggct gcttataatt ggtatgaact tgaagctgtt tgtcagtttt 9960

atgatggaat atttttgcgt gatccgattt gtcaagattt gagtcatcgt gcaagtgcat 10020

tagcaaaccg tgttaaaata aatggttggg acgcagatca ggaaaaagaa ttggaattcc 10080

ttttggaagc aacccgtatc ttccgtaaag tattccctca atggtttgag atggaggaat 10140

aagcatgtgg attgtccgtc ttaagtatga tggcaagttt ccgtgggaga aagatgtttg 10200

tgcctgtgat tcggcagcac atgccaaagc agcgggtcag gcagtagttg acatgtataa 10260

caacacttcc gttgatccag aaatggcagg tgttacaatg agcatgttca cttactattc 10320

ttttgaggtt tactatcatg aaggagagtg gaaatgagcg aagaactatt aacattttac 10380

cgcacgtatt tggcatggtt ggaagctggt gctcctgaag gtaaaccgtt cacccgttgc 10440

acaggtttgt gcacgaacct ctatgatttt tacgatatgg gggaaagcga acgatacgaa 10500

cctgtactgg atgaaatgca tgagcagttc taccacaaag atttggttat ggtgttgcct 10560

ttcaacagta attttggaaa gtacacctta gaagcggaaa aaagccgttg tcatttgaat 10620

gtggatcgtg tgaactgggt aaaagaacgt gtagcagaag aaataattgg ttgacagaag 10680

gatctgtctt tgtcataatc atctcatcga aacagagagg agaaaatcat gaaacagttt 10740

atctgcaccc gttccaccaa cagcgatatc cagaaaggca aggttatcaa aggggaactc 10800

ttccacgcaa atggtaagaa atacattttg ctgaaggact ctttcaacgg ggaacttcca 10860

cgtagcacct tcttcaacta caacacaacg tttgctttag agggagatgt ttgggactgg 10920

gctgaacttc atcctttctg tgaagaagag cagacagaag atcaggaagg gtttgtggga 10980

agtttacgtg ataaactacg tagtaaagca ctgcaagcaa attcccattt tactgaaatg 11040

gtagactgtg ttctgctgga gattgaagat tatgcagaac agggaaaatt ccaaaaatat 11100

tttgaacctt cgagttttgg tttgtgtaac agcccttctt cttacgaaga gatagataaa 11160

cagaaagaag gggtgaatgt agtagccaca atgttgcgtg caaaaggttt agatgtaaca 11220

ttgacaacag cggaatatag cgtttttggg gttatcaaac acatcatcat tagttggaac 11280

taagaggaga taaaaatgca aatcgaagag tctgaagaag aacttatgtt catgggtttg 11340

aatatgtatc gtgtagatta catcatgtgt ggtgtggaat actctatccg agtggggggg 11400

cttcgtccaa gtttcaggct cctttccgag cagggcttat cttagggact tgtcttgcaa 11460

acattgatga tgtgcagatc cagagagtag tgctggaagc agaaaacatt tgacagagag 11520

tgtcacacat attatagtga ttgcatcaac aacaagagga gagagaacat gcaagtagaa 11580

gaattagaag tgatcctgaa agcggctgtt gagaaaggtg caatcgctaa gtatgagcgt 11640

cagaaacaga agcacgacac gcatttcgct cgtgagtttt acatcacagg tgtagacggc 11700

acagaagtga cgctggaatg gtatgtaaac attatgacct tgcatatcgg tagtttcgag 11760

atgtgggtga agaatgtggg gatctctgat acccaccctg gcttccgcgt gagtctggat 11820

ttcacctctt acgaaggtga tcgctgtgcc cgtattggtc gtcgcaattc atttcaaggc 11880

taactggagg gtttatgaaa aaggcattac tgatcgcatt gtttctggtg actcctgctg 11940

ttatggcaga cactgtgccg aaagttaacg gcttctcgga ctcaaaagtt gtaaccaagg 12000

atcgctttgt ccaaactggc atgactgggg ctttagatta catcatcaca gacacaaaga 12060

cagggtgcca gtttgctatc gtctataatg gcggggtcat ctctttgggt tgcttttcgg 12120

agtttaagaa atgaaaaaca ttcgtgtgaa gtttcttgcc tctaagttta ttaatgtccg 12180

agactatttg accataggtc ggatctacaa tgccaaagta gcagatgaga actgtttcat 12240

tgtcgtggat gatgaaggat ctgaactcta ttgtttgttc gagaattgca gctatggcgt 12300

atggcaggtt atgtcggaag aagccgaaaa gaaagagagt ggaatgtgta tcccagaagg 12360

gtatgtcttg gtcccgaaag agcctacccc agaaatgttg gaggcagcga aggactggac 12420

ggggctaacc cgaacagccg aagttgtcta taagtcgatg atcgcggctt cgccgcttat 12480

ggagaaagga gatgacaaat gaaatcaagc attatgttgt tggaattcta tgagtggtgg 12540

ttggatcaag ctatgagtca aaatccaccc gaaaacatcc aagcttgtgg tttatgtcac 12600

gcagtagagg tgtattgtgg ggaagaccaa gatttgtatc ttaccctttt tgatgaaatg 12660

acagagcagt ttgaagcgga aggcttggaa tggaactatc ctttctatgg cgagtgggat 12720

ctttcaggtt ggattccttg ttattggatt gagaagaagt ctggcaccca gcacaagaac 12780

ttccaccgtc ttgcttgggt ctatgaccgt ctggaggagg tgcagcatgg cgaagaagaa 12840

taagatggga gttagtaagg gtggggaacc ccattatccc catccgtttg accccaagaa 12900

caaggcaacg attcatcgtc aatggattga gacacgtatg acaaagtgtc cgatcccagc 12960

agcggagcaa tataacaaaa agtatacagc ggagttcata gaatacactt tcatcaataa 13020

taaaggtaaa gaggtttacg ttgaagaaat ggttctgtgg atcaaatggg aggatgcatg 13080

agttttcaaa ctttctttaa gctgttattg atgttggtct ttttgggatt tctgatcctc 13140

cttgctgatg tggtatcaga cgtgcagaat gagcagatag cccttaaaga acaatgtatc 13200

gtttggaaac aggaaaaaga tgccaataaa ttccttgaca atagctgctt caagtttgta 13260

aaatggaatc ctgataacga ttgaggagtt gatcatgaaa aagtttgttg tttacagccg 13320

tttgtccaaa cagaagaaag gcgaagccca gtatggtatg cagagtcagg aaaacgatat 13380

ccgttacttc ttggagtctg tgccggaggc tgaagtgatc ggagagtttt ctgaatttta 13440

ttcaggtaaa ggtgactgga aacaacgtaa agaacttgtc aaggctgttg agatgtgtaa 13500

agctaccgga gcaaccctcc tggtagctaa agtggaccgt ttaggtcgta acaacgcctc 13560

agtagcaatc ctcttggaaa tgattgacgt taagatcgcc attatgccag atgcggataa 13620

gatggttatc cagcttttat ctgttcttgc tgaacaagaa gttcgcggta tggcagatcg 13680

tatcaaaaaa ggtttggcag tagctaagag caaaggtatt ctcttaggat ctgcatctcc 13740

aaaatataaa gagtctcgta aagattacaa acaatctcga aaaaatgata aaagcatctt 13800

gtatgcagag aagcttcgtg ataaagttac gttaatgagg aaagcaggag catctctgac 13860

tcgaattgca gagatcttta ctgaagagaa aattgaaact gttcgtggca gtaccacatg 13920

gcacccttat acggttcaac gtctgctcaa caaactgaac attaaatagg agctaaaatg 13980

acaaagaacg aagtgattat ccaacaattt cttaaggcat ggttagactg ggctaaagtg 14040

gctccgaaaa gagagaatgt caaacccttc agcacacttg ttgggctttg cgactctttg 14100

tcttactggt tactggaaca agggttttca ttaaaagaaa ttgacgaagt taccgaagca 14160

tttgcagaaa tgcttttcaa agatttccaa gacaccttgt acccatttgg tgacttagct 14220

tatatgcagg attatgagca cggtacgcaa catatgaaca aagaacggtt ggcatgggtt 14280

aaatcaaaac tgaaaggggg tagataacaa tgagcgcaat tttacaagaa ctctatttta 14340

acattgttgt tgctgtggcg gtatgtgtca taggaagcat ctatgcctta gaccgcactt 14400

ttaagaagcg taacaaacat caaaaactct cctacaatac ggtttttgtg tacaactaca 14460

ggccgaggga gggtaagagt ctgggtgtgg ggaatattga tgtacaattt acaaattggc 14520

ctcccactct ggatgatatt cgggaagtag agactcaaat taacgatact tatgattata 14580

acggtgtagt tatgatcaac atccttccag tatccaaaaa caaggagaaa gcatgacaga 14640

atatggagta atgagtgatt ttgaaatcaa caagcgtgtg tttgcagcag tacatggtgt 14700

caagccttta ggctatccgc acaatgcaga tggacgatca gtgggaaatg actgcaacgg 14760

caattacact tggtatgatt actgcaatga tccagcagat gcttgggcta tcatagtaaa 14820

acataggatt tgtatcagcc ctgtaacagg ggaagatggt tttcctcgcg gttggcttgc 14880

gaagacagaa ggtgtaccgt cccgtggaga cgataaaccc ctgcgtgcag ctatgattgt 14940

atttttaatg aaactcgatc gttcttaagg aggtaacatg gatgcatatt ctttacacgc 15000

tttggcagac tctgtgaact ttgagtatat ccaaagagca aaaaccttga tcacaacagc 15060

agccgaagag ggttactaca acatacagat tcaaaaccca gtgaaagctt taggtctacc 15120

gcaaggtaaa gaactacaag cttcgattgt acgcgggatt tgtgacaagt taagatctca 15180

aggttgcagt gctacgttct cgacagactg taatgatctt gtttactcta tgtatgtttc 15240

ttggtaagag gagggaaaat gaaagtagtt gaaggttatc cgttcggtaa gaactttgtg 15300

gcagtgtgga ctgtaggaga tgatttgttc tgtgcgtcct tccatttcca aaatgatcag 15360

tattatagtt acgatcagac tacagatcag tttattgaag agtgtgatca cggatatagc 15420

cctgcgacat tcagacgttt gaacgctaca ttctttgtga tcgattaatc attgacaccc 15480

tcttcggagg gtgttattgt ttgtgcatgt tgaacgagag gagcagacaa tgtttaaaaa 15540

gattctaaaa tggctttcca ctttaggtgg accacgtaat gacaccctta accgctctct 15600

aacaggagaa ccactaacaa tggtcacatt gggaggctgg ggttcaaaag gcttctctgt 15660

gaagcagaca gatgttggta tccaaggatc actggttagc tggaagattt cccaggttgg 15720

agataaccgt caaccttcca tagatctctt aaaaggggag tatgactatt gcttgacaaa 15780

aaactctaaa aatcatctta tagaagtgat ccgtgatcgc ttggaacgcg aaggtggcta 15840

ttacttcgaa acaaaaagaa atgcccacgg aacacttgac tttaagaagg tttattggga 15900

tactaagacc gttcagagaa ttgagaaact cctggaggaa tattatgatt cgaagctatc 15960

atagatggtt ggtcttgcaa gaagaaagag ttttacgtgg gatgttggga tacagcccaa 16020

acacagacga tcttctggat aaaatggaag agtattttaa agaaccccgc gcacacctga 16080

aagaagtctt aggtgcttgg gatggcacac cagaaagtgt aggagagtga gtatgaagat 16140

tgagtcaagt aaactgcttt tagattttta tcaggcttgg ttatcttggg ctgaaaatcg 16200

agatgagacg ggccttgtta tggaagacgt gtttagctct tctgtagggt tgtgtggtaa 16260

tcttgagtat tggttgtatg atttagacct tccagaagac aactggtatt taatcgaaga 16320

cactatgcaa gaaatgtata atcagtttat tgaagcaggg ctatgtgaaa catatccgtt 16380

cggaaggaag tcttatttac tggatgcgga aaatgcttca caacatacag acccagatcg 16440

tctgcactgg gttaagagtc gtcttaaaga tggaggaaga ctatgagcaa gaaacctttt 16500

actatccgtg aaaacctgga caagtccttt tctgttgtta atgagttcaa tgtctctgtt 16560

gccacgttcc cacagaaacg agatgcagaa aagctacgtg atcaattgaa tggtaacgga 16620

cgatgagaca taacgcagca gtcagacttc gatcaagtgg ttggacagta gacgaacata 16680

agacagggat gaatatctac aagatgatta acttcaagga ggagttattg cactgtacta 16740

agtttgcctt ctatcttatg ggagaagaat tgacgaagga tctggaagaa cagatcgaga 16800

ctgttgcaga aggatattac tcatgttcag tgttgattct tgtagacaag gatggtaaga 16860

ttgaagttaa acgagaagac aaactttaca gaggaggggt atgatgaaat ttgctacgta 16920

ttccctaata gcttttatta tttgggttgt tagtgttgta ggaggttggt tagcaggatc 16980

actggtagct ggacactgga ttacggtaga tgtgtttagt tggtggacta tcttcaggat 17040

actgttagca tcttggactg taatattagt ctggactgta ttcgaatgta ccttctctga 17100

gtaatagata gttagtaggt agatggttga tcatagtctg tctacctcac taacattgct 17160

tactccctat cgtgagtaac atgcaataat actatacaaa tcactactcg ctatcgctcg 17220

taaacatatg tttagggtag catgtttttt tgcaaaagtc aatacagcgt tccctatcaa 17280

ttattccttt cagataagtc ctacctatca atcaaaatca cccgttcttc gtagttgtaa 17340

tcgtaacgtt ccggtattat actctctgta actaacaaga ggaggtgtct atggatgcag 17400

taaacaggga tattctgttg atggttcttt tcgtgataac atctgccgca ttagctgtgg 17460

ttattgtgga caataacaag cttgttgttt atgtcagttt cttagtaagt gtggttgggt 17520

gtgttctatg tttctatctt gtagccataa gaggatacta agatttgtct gatggttgtt 17580

atccaggttt taatgtaaaa taataggatg agcggtgttt gtcgtacaag gaggaagctt 17640

tggtggaaat cttcttaccc aagaaaaact tgacagacac gcatcgtaaa tgatatagtg 17700

ggacgatttt aaaggtgact catgaaagtg gtcgaatatg aaacaggata tcgcataaaa 17760

acaggttggt ggatcttcgc ttggtgggtc acatttgcag ggacgggtat tcctgtctac 17820

ttcgaaacca gggaaagagc cgagggctgg ttacaggcga tgcaaagatg ggagaaacgt 17880

ggcaatgatt actaattatc tttataaagc aaaagatctt cgtgacggca attaccactg 17940

gtggttagtt ggagagcaag gtgttgctat cttcccgtgg aacggagaaa ctaaggctgg 18000

catgacaact aaactgaaag aggcgactaa tgtctttctt tatgatatta caaccttcca 18060

ggcttttgct gtcaaacctc aactgattgc cgaatggtga tggagataaa atgactaaac 18120

tttatgttgc aattgataag gtagatgggg tagaatctct attccttctt aatgatcatg 18180

gcgtattaac tatggaacgt ggtttccata ttgatgtgga agatgaagta cgtgcagcta 18240

aggtgctgga gcaaattaca cctccgtact tatactctcc cactgaacgt atgtcacaat 18300

tcgccttgaa tccccagctt ataggagaat gggaatgaga attgccagtg ttgtggatgt 18360

aaccataata acacctgtac cttgtacccc tatggagtgt ccatattgtg gaggtttagg 18420

atatgttctg gttgatgtag gccagcttta tgaatgcaat ccctgtaaaa ctacaggata 18480

tcttccaatg atagaggaga acgtatatga accaaaacga agagattgaa gaaactattg 18540

atgtgttaac tattttccgt cagatggaag gtgtagagaa caatgctcgt gttagcacca 18600

gcttgttgaa catttacaat accattgtaa tgggaaaccc ctcggacaaa gagttgcagt 18660

ttgatcttat gatgggaact ctgttggaaa tcaatcgtga aacgtttagc aaatacgaag 18720

agcaccaaca actaccagaa gatcaacgca atttcattct tagtggtgcg ttaagcgcac 18780

aagagacatt ttacaatacg ttagtggctg cttacaacga tgctttcgtt gtgcatatcg 18840

atgagcgtat tgcaatggtt cgtgatgtga ttgaagatca gatcgaagcg gctctggaag 18900

ctgtggctga acctttagct gctttacgtg ctggtacacc ggaggattta tgattacagt 18960

aggcgatgtt gtaaccttac gtggggaacc cgttatcatg actgttacag aggactacgg 19020

ggactcctgt aaagtgacat ggtttgatgc agagggtcat ctgttacatg ccacattctt 19080

caagaaagct ttggagaagg cggaatgaca acaccgcgag agttttattg tttcgtgaac 19140

ttggaaggca agaatctcgt cacggatgac gaaggttact tcagttattg ggagacagaa 19200

tttgaagcca ttcaaacagt acagaatatt ctgaaagagg cacccacatt tcctatgaaa 19260

gattttgggg tggcgaaggt gactgtcacg acaacagaat acacaaaaga gcgtaaaacg 19320

cttttccata atattgaagt tattcactac ttcacagatc ttatttagga gggaaaatgg 19380

aaacaactta ttgggacgga aaaacacctt tggatgttgg acacatagct ttggtctatg 19440

gtgcatcagc gtatgatcca gactacccat ccattgaaga gggtcaagaa gttacgatct 19500

tagcgaaaga ccgtcagcca agtcgagacg tgtttattgt gcgttggtat gatgagggcg 19560

ggtatattcg tgtgtcgccg ttagtgccac actgtcttca tcctgtatct aaaaacacaa 19620

aacgagcaca agattttgtg aacatgtttg actatctgca cagtatccgt cttaacccaa 19680

aagatgtgca gaaagtgttt gacaaactac aggaagtaga gcaagatgta tctcaagacg 19740

aggcgattga agtgaatctc tggggaaacg caggacttga tcttctcgtt ggaaaaacac 19800

cgttgactct taagctctgg tcttcagatt ataagatcga cgacgatcag ataggtatca 19860

atgttggagt gttggctgta gcaaccaata gcaaagttac gccgcaacag gcgcaatctt 19920

tctactggtt cgatatgcca gtgcaagctt cattttaaat aataggagaa aataatgatt 19980

gcatttatcg taggtttagt agtaggatat cacatccata agtatcaaga tgttatcatc 20040

gccaaagtaa aagatattct ggacgcatct aaataatcta tcaaaagctg catcttaata 20100

ggtgcagcct attaacctaa aggagaagaa catgaataaa gttaccaact tcaaatgtgt 20160

gcattgtggt aagtctttcg gtgatcatca ggctcgtact ttcaactgtc ctcgtaaagg 20220

acgtggtagc ttcaagggtt tcaacccttc acatgtctac tctccaaatt atgacaaacc 20280

agacgaagaa aaaatcgtac tttaaggagg taatatggaa ctcgtacaag aagtttacaa 20340

cggtcttaaa gctctcaacg aagaatggcc tgaacaagct gtgatcgcag ttcgagatgc 20400

agatggagaa attaagttct ccagcgttgg caaatacgat gttgttgaaa cattctctgg 20460

gatttatatg cgtggggatt actgcttgtt caataaaggt atggcacaag gcccaagcgc 20520

aaacttcttc ggcactaaag gtcgcccagc aatagtcaca cgatcagaat acgaggagtt 20580

tttctactct aaacgttgac aaacaattcg tgttgtagga tactaatcct accgcagcac 20640

atgaacccgc ttcggcgggt tttttcatat ctgaacaaag gagattacta tgttgagcaa 20700

aatcgaagaa ggttgtatgg cattcctggt ggacaatcct ttctggtatc ctgtgaagat 20760

tcacgtaggt gagagagtag gggaagatga gtgggagtgt atcttgagca gtgaaatgca 20820

tcattaccaa ccagcaatcc tgaagacaga tcaaatggct cgtattactg ccgaagacga 20880

attgattatc gagttgtacg agagagcact tcacaaatag ccgagaaccc ccttaaatcg 20940

cacagagacg ctctcaaaca gaaaaggcac ccacttgggt gcctttcttt tatgcttcgt 21000

tagattgcgt ttgaggagcc tcaaaatcct ctttagtaca ggttgcaagg taactgatag 21060

tttgtataac cacaacaact ggagtttcaa gcgtctcttc cagtccagag cgaactgttt 21120

ctcttgctaa atcaatattg gctttacgaa cacctgctcc agtgttggta gccaagtaga 21180

ggtttccttc ccctttctgc gtaccgttag agaagtagta gacgtaattg gcgagataaa 21240

aatcgaaagt ttcttgttgc ataataatct cctaataaga aaggggccgt agccccttgg 21300

ttggtttatt tgtttaagtg gtcttcgacc attttaatga aaccttcatt actgaaggct 21360

gttgcagctt tttctcggta aacaaaaact ccacgatctt catagagaag tgtttcttcg 21420

taattaaccc caggattaac tacgaccatc tttgttccta tgttgagggc tgggaacaac 21480

tcccagttga agttgatcac ttgattagag caacctacaa caatcaccat atcctgagat 21540

gtgagattat cgagcatatg gtacatctct gtgtacagcg gagcagattc cccgaagaag 21600

acaacgttag gtttcacgta atcgtactta tccacatcca cacttgcata accgatttct 21660

ttaataaact tccgatccac tacaatttct ggtaggtagc catgcacatg cagaacatct 21720

tcgtgaacaa caccagcacg ttccaaaaga tcgtctacgt tagtggtgat gtttaaaata 21780

cgatcagcgc catactctga gtataaacga gcaatcagac gatgtgcagc gtttggatct 21840

atattgtcca actctatacg acgcttgtta tagaaatcat gggtcttgtt gtagaggttg 21900

tatccttcct catcctcacc tccgataaca ggtaaatcgg gacatgtacg tctacgataa 21960

cctgcctgga atgccccaat attacaaacc tcctctaaat cgtactcgtc ccataaggct 22020

tttccactct ctgtttctgt acggaatgtt tgtacaccgc tatcggcgct tagaccagcg 22080

ccgctgatca ctattaatcg tcttggcatt cttcatcctc ctcgtaatca tcgtcatcaa 22140

aatcatacac ttcatcttca gaataactca cttcaaagta aatatcaaac tctgcaaaca 22200

aacgcaggat gttggacatg ctgtaatagt tatcataagc ttcctctgct tcatagtaga 22260

ggcgtagcaa acgttcagct tcttcgtctt ctgcccattt atgatcaaca taaacagaag 22320

cggcataaat attgtaggct tgttcccgac tatcaacaag atttcggctt aacagttcag 22380

ggaagtcttt acaatttaca ttcagtcttc cgcataacca caaagcaata tcatgccaat 22440

ctgcatcctc tccgctgaag cagcttgcga cttcgccaaa cttggcatct cctaagtctc 22500

cgctaatagt gatcatggtt gaactattgt aaccacaagt ttcgcaattt gaacaactgt 22560

tgtgcacatg aatttctact tgaaacatat ttcctcccat aaaagaaaag ccccctgaag 22620

caggaggctt tgttcagatg ctggcactat accagacttt gtaggtattt gtcaagggct 22680

tcttgatcgc cttggcaaac ataacaacga aagtctttaa cagatggtac ttcattctca 22740

tcccaatccc acattacatt acctttgtaa cgtgaaccag ggaagtcgaa aaatgctgta 22800

tgggtgatgt ggtgtggacc gccccaaacc tctgtaaagg tgaattgcgg atacgctacc 22860

ttcaccgcct ccgtaaacca agctttacga atcaaagcat cgttgataaa atgcgcgtga 22920

cgttccattt cttcccagtt cttccacaca cttttctcgt caagggaaaa tacaagatcc 22980

tcctcaatat acatgccatc ttcccattcg tcaaaatctg cctcaagatt atctacagac 23040

tcgtcaagtc taacaccatc atgagcagag tgtatccacc atcctgcctc cagtaaagct 23100

ttgacaggaa caaaaccttt ttctgcatac tggtcgaatt ctttggcgcg ggagacatga 23160

caatactcct cctcacaatc caacatgctt gcgcctttac gacgcacagc aaagaaatga 23220

ttaccaaaga caactacact gccttcccaa tcatcacgat ctacttgata cgccacaata 23280

ggttttccac caaaaggttt taaatgggtc atcattcctc cttatctaaa aggttgttag 23340

ataaaaacac agcacgagca aaaccgcgag cagttaacga tcggagttgc tttgttttcg 23400

cacttgctcc gcctaaactt ttccagcccc agaagcaacc aatatgttcc acaggcttct 23460

tcacaggttt aacaaaacca ttgccatgcc aaatacaggt tttctttgtg taaccgtcac 23520

gggtaggcat tttagggtgg aagctgcctt cttcatcaaa cagatatccg ccatactcgt 23580

aaggatcgaa atagaagtcc ggtttacgcc acaatgttga caatttgcca accggattct 23640

caatcatcca cggaacgttg tatttatttc ctaaagcttc cacgatacga atgttagcca 23700

aagcttcttc caattcttct gcgctacgtt cgtgcttact accgcaacca gcaagcatgg 23760

tacacggagg gaagccaaag ataatatctg gtttaggaat accgtaatct tcagggttga 23820

agttggaatc aatccatgcg ttcacgtaag tgatgttagg atgttccata cgcacgtcat 23880

aagccccgtg atcaccttcg tcagcattta cacaatagca tttatgccct gcttctgcgt 23940

aacgaagtac catgatccca gatccatcga atgttgataa aactacttta ctcattctcc 24000

ctccatttaa gacaaatatt gactttcttc caatcgctct cggttaagaa gatttgttcg 24060

gaattgatcc acaaatctaa atcttctacc tctggaaggc gataacgtag ctccaagagg 24120

tcgtgttctt cagtagaaat gtaccccaag tgttttgcca tgtcgtggaa ccaagcccaa 24180

ccatgcatat ttttaattac atgctctcgc tgcgtgagga ctttacgttt cagtccgaac 24240

caagaagtga ttaccacttt actctctcgg agccgtttgt caatagctgc tgtttctttt 24300

tcaatactta acagcttcac tcttgccttg ttgagatcca catgtgtcac tttgaagtaa 24360

ctttgtgact gatcacacca acccatatca ataacctcct tcgtaaggtt tcaccatgtg 24420

aagatttgct ttaaaacgtg ccacgtaatc atcttggccc atttgctcat acagttttac 24480

cacaacttgg aagttttccc aagcatattc acgaaaccat ccactggtga tcgaggtaca 24540

aactttggat aaagccatca taaaactttg tttaggagtt ggaccatcta catcttcccc 24600

aaacgggatc tgtgaacgct ctaatgccag gacacaagct tcttcgtaaa cacccagcaa 24660

acggatatgt ttaggttgtg caaagaactt ctctttactg gtcatcactt cgctatcgtc 24720

tttcatgtat gatttataag cgggatcacc caacacggca acagcttcat ggatggtatc 24780

gtgatcatac acataaggca cttcatcacc cttgaagaaa tctttacttt tcacgttcag 24840

attagggtgc ccgtaattat aggactcctt ctgacgcagt ttgtatgtat cttccaactc 24900

ttcagggatc ttcgctccaa ggttacgcat gaagtggata tccttcattg ttttcaggaa 24960

gtgaggactg tccttcttaa aacggtgact cattttcagg agatacaaaa catccaaagg 25020

tgcaaccaca ttgtaaccaa tatgtggcag atgatctgca ttctctttaa tgtattcgat 25080

caatgcttct gtagaatccc ctggttgagc aatatcgtat tccagatgag cacccgcgat 25140

cattttgata tgccctttgt tacctttacg ttcaacaatt tccacgaaac gatctttcga 25200

gaatttctgg gcttctgctt gccactcttc ttcagtgcag aggtaatcca gatctacgca 25260

agcacgatac agttctacac cacgagaacg acaaatcatt tccaacgcac gactaccgat 25320

taatactttc atatatcctc ctctcttagt tgatgagaca agtctaccag gttcttcgtg 25380

tcttgtcaac actcacacaa taaaaaaccc gccgaagcgg gttattattt attagcacat 25440

ttccgaggaa gacacccaaa gaccttgggt catcagaccg ttttcgtcaa tctcaacatc 25500

atagtctccg cagatatagg tgaggtaatg ttcttctgcg tttgttcctg tcgggtaata 25560

agtttcccca ttcccgtaat caccgatcca cgcttgttct tggaactgct cacaaagttc 25620

ttcaatctcg ttgcggaact tctgttgtga tgcttccatc tctttaactt ttgccttgat 25680

taaggcgata ccttctgaca tgatccctcc ttaacaatta ctgctgtacc aaacaccgtc 25740

agagtcaacg gtgaaaccat catcagcttc gccatagcaa gcagaagatt gccaatcttc 25800

aaaacagatt gcattgtttt ctaagctaag accgaaacct tccttttctg cttcttttgt 25860

aagttcatct accaaggaca tgatagcttt tgctttgtct gctaatgctt gtgcattacg 25920

atcactcatt ttatttctcc ttattaacaa ccgagggaag atgggtgcca gttggtccca 25980

gtgtattcgt tctcatcttc tgggtcataa gtaccaccca ttccatattc aacagagaag 26040

tgaaaacttg tgccagtttc atcagccaat tgttcagctt ctgcaatggc ttcgtgtgct 26100

gctttcagtg cttctgctac tgttttatac ttcgtcgtca taaaatttct cctctcccaa 26160

agctaatacg atattgaatg cacgataagt gaattcgttc agttctgtgt cagagtaaaa 26220

ctcaaactgc acaacaccgt cttgagaaga cataaacata ccgatctgca cagtgccttc 26280

tggaatgaga tgttgatatt ctggacgaag ggttacattg tagaaataaa gttcaccaac 26340

gcccatctca tcccatgctt cccaattgct tacgaagttg ctttcgtaac ttttcacttc 26400

attaaattcc gccattctct tcctcctcac gttttacttc ttcaattgta taacacatct 26460

cggtttcgag agtggtttgt ggtccacttg tgcgtatcca tccataaccc agtttaccag 26520

gtttgttaag atacttaata aaactcattt tgtggatagt cactataaca tgttttggat 26580

ttttgtcaag aacttttccg atatcttgca acaatttatt cgccagaatt tctgccatac 26640

gtgcaagctg cgtgtcttga agatccccta caccaataaa ataacggttg atctgttcaa 26700

tatctgcttg ggttaaatct ttatctacga agataatccc atcattcttt gaacgatcat 26760

cttctccgtc ataacgaaag attaccaaat ctctggcaaa acgcaaaccg tgatggacaa 26820

taccctgcat gattttttca atatctgctg taacatctgt gtcctcgttt atacgactaa 26880

ggttaaaacg attacctacg tttgcacgat ctaacttaat tgccactgat cgcccccttg 26940

accaacggtt taagaaaagc ataaagttct tcctttgttt taccacgtaa gttaaaacat 27000

ggaacgttat tagctttagc gatgagccac gccgtccgtg ttccgcccat aacatctccc 27060

ttcttatcct ctttggaaca tgccaataag aataaggaag gcacgggatg ctccagatct 27120

tgtcccaaca cctgatgaac gttacgttga tgcaatttac gtgcaccttg tttaagttta 27180

tcaaatgccg gatggatttc ctttatccac tggagactga tagcatagtt catactgtca 27240

ggattagcaa gcactatttt ctcacctggg atatcattac cttcaaactt ctcccaaggg 27300

atataaatct ccttgagagt tttgttttta gattcttcta caccagtttc gaaagcagag 27360

tcactaccgt ttgcattacc tgaacgtaaa gtaaaaccac agttagccaa ccactttgct 27420

acagcgacca ttaaatccca ttcttcgtgg gttatctctc gacttcccac acctgtgtaa 27480

aataggttgc tcataagcca cctcaacagg ttatttcaaa cgggtttctg ttagtcatga 27540

tccacggatc accagaagga tatggattat aaggacgaaa aacgtgtggt tctttgcccc 27600

acttcttcct ttcctcctca aaaatacgac gaaattctgc atattgatca tcgatcatca 27660

gattcggagc gtccgactca accttatcta catccttccc aattatgatt tcacgtacca 27720

gttccagatc ttccgtctcc agaataattt ctttacgaat ctcacctaca gattctacga 27780

ttgtaatttt atacattagt cctcctgtaa agttgaataa aactgaagtc ccccgtctgc 27840

atcaaaatag gcaaacgtta agttgttatt gaaaacagac ccagtatcca tataaacacg 27900

attagcattg atcaaaggtt cctttacata agaatgcccg tgaaacacat aatcaacacc 27960

cataacaacc ggaagtttca tgccttcttt agcacactct accatgtcac gatcccacat 28020

caaagcttca gcgaaaatag cttcttttgc tttgctcata cgcagatgtg gtatatctgt 28080

caagggaaga tattcaaaag aggaaggata tccaccatga ataaacccta acttcatacc 28140

tcgatagttc acaaccaaaa ccacagggtt gtcctcaacc atcgtagcga gcagagtaga 28200

tccttcttca tcgcattctg ccaacactgt atgaccaccg ttctgatacc aacactcata 28260

gtaacgacga tcaccttcca gtaaaccttt aatcatcata tcttcgtgat taccacagag 28320

agcatagcga ttctctttgc tacaaaactc tacaacacat ttgaagtttt gtttaccacg 28380

atctgtcaaa tctccgagag acacaaccac atctgtatct cggataccaa gataattctt 28440

acctttcaag taaagatcgt agcaaccatg caagtccccg ataatgaaga ggtcttgatc 28500

ctgctccacg gtaatctctt tatataaaat catatctcct cctcaaaatc cagaaaaata 28560

aaaatgttta ccttcccttc gagatcctcc tgtaatacga gttcggcaaa ctctgaataa 28620

gctttctctt caaccttctc aatatgtgaa gctgccagct tgaaaggcgt atctccgtct 28680

accttaacaa gcaaaaacag agaacgcatt tcaacgtctt gtttggtgcg aggaaggaaa 28740

tctacagctt caaccatgcg ccaccctaag tagccagggg tttgatgtat atctcccaaa 28800

tgcttctcta agattttacg cccttcgtcg ataagagcct gttctgtagt aatttccatc 28860

ccttcctcct caaatgatta acgctaaagc gtggctgaac ataggttcat tgatcacgtt 28920

cgctactgtt acgtatgtgt cttgcaccca gtaggattca acacgacata cccgaacacc 28980

atttatgtaa aagtctgaac aagatttata atcatcgcac aacttacagc tataagcaat 29040

acccaatgtg tcataaacat tccttacagc taaaaccata tcctctccac agtctttatc 29100

agaaaccatg aacatggtta agaccaagtt atttgcgaag gtgatactat cttcaatctc 29160

caccgattta caaggtagca atgagaaata actaccttcg gtaataattt tagcctccac 29220

ttggtttaga atactttgtg aaggtgctgt tacataaaac aagttctcgt ttggattctc 29280

aacgttgcta cgaacaacac aacgagaagg atttaatgga ttgaatacat ggtcaaaatc 29340

tacacattcc ggtaggtttg ccactttgaa agttttgctg atgattcctg ctgctgctaa 29400

aatgatacca gaattaatct tacctgtgta agtagaacgc ataactactc cttcgatcac 29460

tctatacgag agaagttctc ttctcctaaa tagataattg atctgtttac attataagag 29520

acattccagg aatccccttc atgcctaatg agaactcctt ccacatccaa aagcaaatca 29580

gaagcaaaat tgactgcctg tgtacgtgtc gggaactttt taacaactat ctcttcattc 29640

tcttgcatag cttcctcctt aaaataccct ccttgccgaa acaaggagag tacgaactac 29700

ttcaagcaat atcaggttgt ggtgatagtg tcttcttgga tcgtatcatc catctctgcg 29760

aacagatccg cagcagaata gttaccacct gtccaacgtg acaaaaggat cgcaccaatt 29820

tcttttgcat gtgtttctga ttctgcatag acattagtgc cagtatcgcc tttctttgct 29880

tcgtttgcgt aaacgcaata ataattataa gccattaatc atcccaatct tcaccgtttt 29940

gagcatcgac caaggcttta accatgctac cgtaatccac agtttgattt gcagaatgcg 30000

tcgctgtaac tgttatggca tctttaaaga ataccgccat agctgcttct gttgggttca 30060

aatcaccttc cagattatga gcaataccta agttaatgcc tccgtcctca tcatcaaaat 30120

caatagaaac ggtcatagta aatttagcca tagtgtgctc cttaaacgaa ataaaatggt 30180

tgagttagac ccaaaatttg ttttggatcg tatacgtacc aagcatacgc tgtcttatct 30240

gttccacccg ctacgaacga aggacgttcg gagagtacaa tcaaattcgt aggtgggttt 30300

tcttgatgaa acgctcgacg tttcttcgac tcaaggaatc ccaggcgaag caggtagact 30360

gacacgtctg cttctttcaa gctcttttcc aagaactcta atgccagctt gaatggaggg 30420

ttcgtgatta taatgtcggg acgattttcc catacgtgac taaaataatc cttaccttct 30480

tcaagctcac aatactgaga accttcagga aagtgacggt aaatagccgc tgcttgtcct 30540

ttacacggtt ctgcaaatgt aaatccttcc tctttcaaat tggtaaaatc aataacacta 30600

aacagcgatt caatcgcctt ctgcggagtc ggatagtaat cagactctgg acgatcatag 30660

cttaaagcca tttaaaagct cctccttagt tatagcctca tcttccacga acgaagagat 30720

ttcattaagc aattgcatct ctaagtttgt aataccaata gtgctcttat ctgttttacg 30780

agaaaggatg ccttcaataa cttcctcaat agacaagctc tggtcgtaga acttaagtaa 30840

ctggtaatcc acaggggaga ttttatcttt tatcagtaaa aactctaaaa cgtgttgcat 30900

gttatcgtca tattggagtt gatactcccc aaatgcatta acactataag aatccttacc 30960

aaaccacttt cttttctttt gaatcacgta ccgcgtttga atcaaagact gcaaacagtt 31020

tgaggcacgt ttacaagttt gttggtaagc attaacacag ctatcgacga atttaacagg 31080

aacgttcaac ataataatct cctctgtgta agaaatgccc ccgaaggggc agttttatta 31140

agcagcggga ttgtcgaaat ccgctacgtt tacgagatta atattttccg gtttagaagt 31200

caagtctttt tctacagcaa cctcttcttc taccgtctcg tcacctgcca cgtcacgagg 31260

agtttcttca ccagcaggat caccaaactt gagcaggttg atcagttctt gcttggtgaa 31320

gttttcgaga tcttcgtcag tcagttcaat gatctcatct aattcttcaa cgaaggtttc 31380

gccaccagag tatgcgtcac gcttcttggc aacttccata gcagcttctg gagaagcacc 31440

tccatccagt gctgcgagcg cgaaatcgga accactacct actgcgtagt attcttcgtc 31500

gattgggtat gcacgggtag gatcattacc ttcatgcaag aaaatacgtc catctggatg 31560

aacaacgaga gcaataaact cttcatcttc acgatcaggg ttagaactaa tcatcaaccc 31620

aggtactgct tcacgagccg cttcacattg cagattctgt tcgaaccact ggaagaaagc 31680

taaaacggaa gtcaaacgtc ctgcaccgcc tacaaggtaa tccccgatct tacggacttt 31740

cttgaaactg gttgagtcta cacgaccacc gaaagttact ttaccgtcgc taaggatctt 31800

gtttttggtt gcgataattg tagtcattct gcttcctcct ctgttaatgt tttaagaact 31860

gctttcccgc cgttgacata cttgcgcaga tagtctatcg cacgtaatgt ctctttgtca 31920

accagtattt cgtttgtgat cgccagtgga tgatttaaaa gatcactaca ctcacgaaca 31980

agcaatgctt tacgattgaa catattgctt aagaacagta acttcgcctc ttcttcatcc 32040

aaatggagaa gttcaggatt ggagccagct attcctatta tacattcata taagccttct 32100

tgactatcag aaagttcttg aaaatgggcc aataaccaat cttcaccttt acaccaagcc 32160

acaaacattg acaacttgtc tcggagggta aggttctcta aagttagtgc aatttctttc 32220

tcaatatgat ttacgcaaat ctgtcggaac tcattaggct tcacaagccc agtttggtaa 32280

cactcgtcaa taccagtccg atctataatt gtctgtacca tcgaccctcc ctttaagaaa 32340

gcatccttgc tttttgttat taataaattt ctgtgatgaa cttagacaaa cgagagcgtt 32400

tgttgtcatc tgtggttaac tgcacataac cgataaagtc taccagaggg acacgaacac 32460

catcttcacc ttcaggagca actttgtcaa gaagatcttt cagaccgttt gcacgctttt 32520

tgatagcgta ggtttgacga acatctccaa gaacaacaac atgagtgttg atccctgcac 32580

gctctaccag aagttttaat gtttctggag acataagctg tgcttcatca atgatcacaa 32640

tagtgttgtc cagggtagca cccagtaggt agttcgggat attaagttca atcttaccgg 32700

aaccaatatc attctctaat ttgtttgcag tcataaactg ctggaagatt cgtttggtgg 32760

attcgtagtg cgccgccagt ttatcagttt tatctcccga aaggaaacca atctgatcat 32820

caccaacttc agtcgggttt ttaataaaaa gaagcttacg gaaatctcct gctcgaagca 32880

tctgcaaagc tttccataaa gtcacggaag tcttgcctgt ccctgctgga gcatttacga 32940

ctgtaagatc attccagatc atactttgaa cgatttgctg ctgatctcct tctggtgaaa 33000

acccatctaa gcgatagtca tcatttccaa ctgcttttgc agcataacga ctttctactt 33060

tcgcattaga agctaacttc tggcgcggtg atttagcggt agctgttttt cccatagtag 33120

cactccttat agaaggggct tgcgcccctt tgttagttgt aagaatcaag agtcccagat 33180

gttagaacct tgattactcg ttctgcacgg tttggcgttt gagagtacca tttactggct 33240

ctcatgtttt taccagcttg agtatagtac gaattactga ttaaagtcaa gctattttta 33300

aagccttccg ttccggttaa ccccaactgg aacaccatat tgatgattgc cagtttacgg 33360

gtttcactca ccttatcata gattggagca agaatggaac tatttttaat ctgttttttc 33420

gccttttcaa catcgtcagc aaagatagca cgggcttctt tctctgtgat aactccgtta 33480

gtcttgcgac caacaagagc atccagaata cgaatagctt ccgctttatc tttcttttta 33540

gttaagaggt gtccaatgcc gactgtccaa tatcccaagg tgtctgggta aacagtcagc 33600

ttcagtcctt catcaacagc caacatgtca aactcatcta ctactttttt agccatgttt 33660

tcctttattc gtcgtcttct actccgtgat ccagttcgtc aaacaataaa tgttcaggag 33720

aagtctcacg tttcggtttc gtcttctttg tgttggactt aagctcgttt gctaaagcac 33780

gagaaaagat cttacgtttc tgtttacttt tcatttatat tactccgcaa tttgagccat 33840

gaaatcacgc agttcggtaa aaccgcctac gtgggttact gctccaagct cttccacaaa 33900

aatctgcggg aaggtgcgag gtgcaattcc tgttgctgcg atcactgctt gagtcatcac 33960

ttcaaaatca taatcctgat ctaatgtcag atattcaaac tctttttcac gcatagttaa 34020

gagttgtttg gacatttcac agaactggca atttggtttg ccgtaaatta caaaattcat 34080

gctctctcct tattaagtaa tactgctaaa gattcttcaa taccttgtcc gacgcttgaa 34140

ggttcattcc aaccagacgt aattagtata gactttgctg gtcgcatcgc cgccgcatta 34200

taggtttcag cagaggtcgg gtgtataata acagattcag gcatacgtgt caatatcatt 34260

tttgcgaact ggtgccacga aacagtgatg ttcccagcgt aatgcaaagt acgggtgtta 34320

tatcctgttt ccaacacttg gaaaatcatc ttagccaaat ctggggcata agttggtcga 34380

gatgtttgat ctgcaactac cgagaagtct ctttgaccat cgttgtaacg agataccata 34440

gtctttagga agttgttacc aaactcgcta tacacagaag ccgtgcggat aataaaagct 34500

ttatggtaga taccgcgaac agcagcttca cccaaacttt tagataaccc gtaaacattg 34560

atagggttgg gttcatcctg atggaacaac ggtttatcgc tgttgaacac ataatcagta 34620

gaaatatgga ccatatcaat atcccaagcc ttacagaagg ctgcaatatt agctgcccca 34680

tgtaagttta cagccatagc tgcatcaaca tctgtctcgg ctttgtccac agcagtgtat 34740

gccgcacagt tgataacacc gttaatattt ggggtgtcca tgatcgcttt tcttacagct 34800

tcgaaactgg tgatatctac acgcttttct ggatacacca aagtcacatt ctccggcacc 34860

actttacgca gtgcctgtcc caattgacca ttcccaccca atactaaata tgttttcatg 34920

atcccataag ctccttcagt gttttagctt gtgcatcctt ttccgaaatg ataggtacaa 34980

agttctcatc attgaaaatc atatcaaaac cccaatcaat attcacagta ggatcgttcc 35040

acaacaaact acgttcagat gccggatcgt agtagttggt gcacttgtat tcaaagttgg 35100

cttcataaga ggttacaaca aaaccatgtg caaagccagg cggaatccaa aactgtttat 35160

tgttacgccc actaagaatc acaccttccc attgtccgaa ggttggacta ccttcacgaa 35220

gatctacagc cacgtcaaaa acttcgcctc gtgtgcaacg tacaagctta ccttgcgggt 35280

tgtctacttg aaagtgcatt ccacgaagaa cacctaaagt agagaatgaa tggttatctt 35340

gaacaaacgt ttgtttgcca ataacctctt cgtaaatctt ctcgttaaac gtctccgtga 35400

agaaaccacg atcatctttg taaacttgag gtttaaagac aaccagacca ggaatactta 35460

attcttttat tttcatttac cctccgctaa ttttaataga tattgcccgt actctgtttt 35520

ctttaatgag ttgccatgat acaccaaagt ctctaaagaa atccaccctt ttttgaaagc 35580

aatctcttca aggcacgcaa tcttcaatcc ttgacagttc tcaatagtct gcacgaattg 35640

gccagcttcc atcagactgg cgtgtgttcc tgtgtcaagc caagtaaatc ctcgtccgag 35700

aacttccact tgtaacttcc tgtcttcgag gtagctgcga ttgatatctg tgatctccag 35760

ttctccacga tcggaaggag taacttccag tgcacgctca acaaccgaat tatcatagaa 35820

gtaaagacca gtgactgcat agtcagattt gggattttca ggcttctctt caatagatat 35880

agcttgacgg ttgctaccaa actctaccac accgaaacgt tcaggatctt taaccagata 35940

accaaagata catgccccac cccaaacatc caccatctcg ctggcttgga taaggagttt 36000

cccgaaactt tgtccgaaga acaagttatc ccctaaaata agggcaacag aatcttcccc 36060

aataaaatct ttaccaatga tgaaagcatc tgcaagacct cgtggttccg actgcatcgc 36120

gtaagtgatc tttatcccaa acatagatcc atctccaaga agatattcaa aatcttcctg 36180

atcgcctggg gttgtgatga ttaagatatc cttaatatct gccaacatca agactgataa 36240

aggataataa atcataggtt tgtcatacac agggagcaat tgcttgctca cactgcgtgt 36300

taccggatgg aggcgggttc cacttcctcc cgccaaaata atacctttca taaccctcct 36360

taaatctttt ctctccacca ttgagggttg tttaaatacc actctatcgt agagtaaagg 36420

tcttgttcaa atgtggagtt tgcactccaa cccagttcag attcaatctt agcagggttg 36480

atttcataat gcaagtcatg tcctttacga tcttcaacaa aagttattaa acttgaagag 36540

gggtaggttc cactttgcgg gtgtacggca tccatgtaat cacaaatcat atagatgatc 36600

tccatattgg ttttaggatt acgtgtacct acgttgtagg tttgtcctgg ttcaccgcca 36660

gaagctactt gaagaatagc tcgtgcatgg tcctcaacaa aaagccagtc tcgtacctga 36720

tcacctttac catacactgg gatttctttg ttcgtcaaga tgcttgtgat tacttttgga 36780

atcaattttt cagggtgttg gtaaggtcca aaattattgg agcaattggt aatgacgaca 36840

ggaagatcat aagttcggtg ccaagcttta acaagatggt cgcttgcggc tttagacgcg 36900

ctgtacgggc ttgaaggagc atatgctgtc tcttcggtaa agggagtacc agagtcagcc 36960

atatcgccgt acacttcgtc tgtggagacg tgtacaaaca tcttcagctt attccttggg 37020

agtagtctcg cagcttccag taaattgtat gtaccgatga tattggagtc gatgaaagct 37080

cgtggattct tgatagagtt gtccacatga ctctctgccg ctaagtgaat gatgaaatca 37140

gggttgcatt ctaccactaa acttttaact tgtgcttcat ctaccagatc tgtcatgctt 37200

agtttcgcat gagtaatatc tccgacattt tcaatatcgg ctgcgtagcc cattttatca 37260

ataacaaaaa cttcattttt aggatcttga caaagagagc gcactacggc gctcccaata 37320

aaaccaagac cacctgttac cagatagcgc attagttctc cttaccagac gttttgtgat 37380

actaaggttt cagtctcaat gttatactta ttaagcagtt caaaatagat ctcattcgct 37440

tttttattta aacttgtgag gtctatagca caagcatcac aaccgctaca cagcttaata 37500

ccgaagacac aatcttcagg atctgcgtca taccacggcg aaatgttttc cagacctaaa 37560

ccttcgtaaa gaaaccaagt aaaatcccca tcaaactgat cttgcacttg tggatcgttt 37620

tcaaaggttt ctttccattt gtcatacgat gcgccttgca ccatcttcca ctcgatatca 37680

atccccatct ttttctcctt cttcttggtt atcttcttca acaaccaaac cactaccaaa 37740

acaatgccaa cacgggctgt actgctccgc actaattaag gcattacctg taccaccaca 37800

aatagggcaa aaacgaaact cactcattat cttcctcctt tacgatttca aactttgcaa 37860

caccgttaac cacaatatga ccacaagaca taacaccaag ccaagtctct ccgtttttct 37920

taagacgttt accatgcaca tcacaaaaac cgttctttaa ctccgaggct tcataggaag 37980

cccctttgtc aaatacgtct ttgaatgtgt cgatcaatcc tacacattta agtttcatta 38040

gaagtcaaac tccatatcat cagaaacatc tttgttagcc gagttcagtt tataagctgt 38100

aacttggatc tcttgcgctg ctgcttgaat ctgatctggg tttaaccaaa tatccatcca 38160

aggtagtggg ttctgtttag gagatttaaa gctgtgcttc atcttcagac gacgataaat 38220

tggagccgca ttgaagtaca cccattgacg agatagttca gcattcagcc caacgataaa 38280

acgtccttcg ctaaacagat aatcacacca ctgctcttct tgagcaataa ctgtatccag 38340

aatagttgta agttcttcga tgttatcttc gtagatcttc ttccacgcag gatcttgcat 38400

cagagcatca atgacagcat agtccatttt aacatgcagg atttcatcca acatgatttt 38460

ctgaatcagt ttacaagcac caacgaataa ttgttgttct gccagagcaa acgttgccga 38520

gaaagaagag atgaactgaa taccttccag accaatcagt gctaccaggc ctttcagaac 38580

taacaaacga agcttatcat tagcttcaat cagacccagt gcatatttgt gacctgcttc 38640

ttccaactct cccatgatct ctgcaatgat acccatacgg ttgaagatct tgtcgttctc 38700

catgatctgc tcaataactt ctacagggtt tggagtacac tggcgaacaa tctccgaata 38760

agttagagcg tgaagcactt ccatctcact ctgcttcatc atcatagcac atgcttcatt 38820

gttgctcaaa aacggagaga acaaagtgat gatggatttt gccagagaat cattctccca 38880

ctggaacgac aagttcttga tcataacatc gtagttgttt tttgaacacg ctacaaagtc 38940

tttacgggtc tgactaagat ccacttcatt ctcatcccag tcttgggctt tctgaagttt 39000

atacagacgg tgcatttcag ggtaagtcac attaattgag tcaaagatcc ccaattgctc 39060

acccaggaat accggatatt tccccgtttt gtaagtctcg ttatgtaaat taattgctgc 39120

catttaaatc ctccactatt tcacaaaaac ctcttaagct taaaatatct tcttcctcaa 39180

aatcattgct ccggtagaaa gcttccagtg cttcccatac gcttctacca agtataacag 39240

tcactgtttc ttcacttctt gcccttgtta ttgcgtaagc tgtggcttct ggataaaaag 39300

attcccaaat ctgcatgtta gctatttcaa gacccccaag ataatggtga caccaagtag 39360

gggtaaaatc ttcagggcga atcaacagag gcttaaaccc aggtcggtag attggggtta 39420

ctccaggtaa aactataagt gtgctcataa tcctcctcaa taaaaagggg ccgaagcccc 39480

tatattgcta cattttacaa gcttcacaac caccgtcaga atcttcttgg gccatagatt 39540

ccgctgattt ggttcggctg ttcaggtagt aaagagattt accaccaagt tttgcaaatg 39600

tcaagataaa ctgcatctcg gctttcactg ataaacgtcc accttccatt ttggaataat 39660

ccaaccaagc atcacaggag ataccctgat ccgtaaactt agtgaagagt ccatacatct 39720

cggcaatatc tttagatggg gtgtcccacg ccgactgata gaacattttc agtttttcgt 39780

aatccggtac gataaacagt acgttacctt tctgactctg tttgtacatg atcgtgtcac 39840

gtacagggta gagtgagttg gtagtattac tcaccaagct acttgattcg ttcggggcca 39900

ctgctgcaag cacactgtta cggataccac cttgttcaat gataccagca cgtaagcctt 39960

cccaatccag cttcagttca aagtccccaa tagtatctac cgctttagcg taggtgtcaa 40020

tagggagcca accttgaggc cacttagttt tatccatcca ctcggcatta cctttctctt 40080

tcgccaagcg cagagaggct tgtgccaacc agaagtaatg acgttctgct aattcatgca 40140

tcaacttctt cgcataagta tcagaatagg ttacaaaatt gcttgccagg taatgtgcaa 40200

ggtttgtaat acctacgccg atagagcggc gagccttagc tgtgatttcg ttatgtttga 40260

acggatattc catcgtctca ataacgttat caatcgtcaa tacagtataa tacgcaacat 40320

cttcgtattg ctccggcgta actcgcccag caacaataga ggacaagaag cataaagcca 40380

attcttgatc ttctgtgttt tcaccagtaa agagatccgt cattttatgg aacgctttag 40440

taggtaaaaa gatctcctga cataagttcg actgatacac agtgtccaag aaaggagtgt 40500

gattgtttac gttatcagca aagaacatat aaatacgtcc agtttctgca cgttgaacaa 40560

tgatttcagt caacaggtca cgagccttaa caattttctt tttgatgcga cggcgactaa 40620

ctttatcgta gacagcatcg aactcttctt cacttttgta aagtgcttcg taaagttccg 40680

gtgcttcgtc acaactcaca agcatccact cttcaccttt agccgcacgg cgaaggaagg 40740

agcggtttac aacaacagag taatccagct tatcaacacg cttatcgatc ggagtggttg 40800

ggttcttcag tcgaatcaga tcttcaaaat ctggctccaa aattgtgaac gtaaccgttg 40860

cactaccacc acgactcgct tgtttagaag cccctacgct gtcttccatc cacttcagat 40920

aagggagaag accaccgtga acgatacggt tgttcttaac accttgtcca gttgcacggg 40980

tctggaggtt aacaccaatc cctgcattat ttaacgtgta ttcgtgtgcc agatagtttg 41040

caatagccaa agactctgct gtatcgtctg cacggaataa acaacaagac gcgctgcctt 41100

tgatcggagt acgtaacgtt gtcagatatg gagatggtaa gttaatctga agatcactta 41160

catatgtgta cagctttttg atatcgtcaa tacgacgatc tttaggctgg atctccataa 41220

cacgcattgc cacaccaata aacaggaatt gcggtgactc atgaacaata tcattcacag 41280

catcacgaac caggtattta tctgccatct gacgaagaac ggaataaccg taggttagat 41340

tcttgttgtg tttgacaatc ttaccaatct ctttgatttc ttcttcggta taatccatct 41400

tagcccaatg tccacgcttc accatatcct ggtagaaggt gtacagggaa gggatactgt 41460

ggtatccccc gtgagcctgt ttgtaaatca taccgagaag caatcgacct gccatgtcgc 41520

tatacgcttg agtctttttg tcaatacatg cagcgatcat agctttatgt aaatcacgag 41580

tagacacacc atcgtaacaa cgtttcacag cttctaattc aatttccgac cagttcagtt 41640

tccctgctgc aaattcagac catttacgca gtttttcagg attaaactct tctgtcttgc 41700

catttgattt agtaacgtat cgaatcactt ctcctcccta tcttaactat gaatcccaag 41760

cacgtctaca tcgtatccaa atttcatttc gttaaacagt ccgttcgcat aaaggaaccc 41820

ttcaaaatga tttatatcgt ggaaaccttt cgctttagct ttgcgatctg cttcttgtcg 41880

ttttggtgat aagcgtttgg aagtatctaa aatcactgta acattacagc ctactgtctc 41940

atcaaagtaa acttcaatct cacagccttc gtatttatta tcggtcatta tccctctctt 42000

aaagtgaatt gtcttctgct cttgcaggat cgtaggaatt accgaaatcg tccatttttg 42060

tgtgagtcac accccaaccg ccaaattctt catcaccata atgaaaaata tcattcttct 42120

ggtagatgtg tttgaaagga cagctttctt taacagcaaa agtataaaga gttgtgttga 42180

tataaaccag actgttgtcg ttgttactac ggatattaac attacgttca acagagaagt 42240

tgccatcttc gttcatacca aggaacacca gagcggcacg aagtacatct gcgtgacgat 42300

cgtggggaat atgaccacgg tatttttcgg actcttgttg ccagatcggg gtagacttaa 42360

cggtagacat gctcacggta gcccaagtgg tagtgtttac taatgacata attttctcct 42420

tttataagtg ggggatctca cctcccccaa cgttttgcta tactacacca aatctcatac 42480

taaagtcaat aagaatttac tcgaatcacc atttagtatg attatttaaa agctggcggt 42540

gctggataaa tcctttgaaa tttccagacc acaaacaaca tttacgatcc atgtgtgtaa 42600

taccttcttc ccaagtagta gggaatggag gcatattaac accagctttt tgttgtttta 42660

atcccgacaa atccatttct ttcattggag ttgcttgatg ttcaaacgga gaagcatgga 42720

ctttacgtcc agtcagcaaa cgcccgtaga tatccaaagc tttatctttg gttgcattca 42780

gacgacgata actcacttgt gcgcaacaag aagaagagat tgcaagtgcc tcttccagtg 42840

acagcatgac agtatcgcca ttttcatctg tagtgctata cccagcgaat accaaaacat 42900

catccccttc aagaccgatg ttctcgtaga cgtgatctac gtaaggggtg tgccattgcc 42960

ctggagccag tagttctgga gtagaggctt cataagcttc aaacataact ttagctaaag 43020

cttcaatagt cggatcagca tcagcatcgt cacgtaacca ccagaagttt tcatactcgg 43080

ttgctgtcag aacagtcttc attctctgga atggttcaag aaggcgatta caaacctgct 43140

tcgcgtaacc agcttccgcc atttgttctg caaagtttgc tgcactaagc gcagctaaat 43200

tccaccactc acgacccgaa taacctaccc caatgagagc atcatactct acaccctgat 43260

cctgcatacc aggttggtgt tgaccaaaac gaacaggtgc agcaggttcg ttgcgaacca 43320

tctcaatcaa agtcttgact ggaatagcac ggcttgaagc cgcgttgcgg gagaataagc 43380

ggtgcgtcat caactcagaa tggatgatac gtgggtattc cagttcgaaa gttgtaatca 43440

cttgcccttc tgcgttcata gactgggcga tgacttgtgc actgatatta gacattaatt 43500

tctcctcgtt cttttaacat tgaataaact tgccaacagg tttctgcttg attgtacaaa 43560

cctgtgacaa ctacgtttgg tttctttttt aattcttcca aatacgaatc tttatacatc 43620

gtattgtaat aaattttaat aacatgatct ggaaaataca acaggttttc tgccctgtca 43680

tctatggcaa tatcacaagg caacaggtat ttttcttttg tggcaaagaa accgttacca 43740

gaacctttac caaaatcaat ggttggcaaa gttcttagaa catgtttgtg ttttgagcta 43800

aggtgtccac ctttagtcac acttgcaata agtgcattat ctccctcttt cacaacacct 43860

tctaagaagt tagccatagg tttgatacat cccatcgtat catagagatg aggattatcc 43920

agaaagccgt aagggtcgat gttaaacttc tcacggaagc ttgggaagta tttagtaaga 43980

tcgtagtcaa cattcttgac agcttcgtag aagcctacac aattaccact aactttaacc 44040

aaccattcca accaagcaac atccagtgga gcaaccacca aatcccaatc acaaactatc 44100

ttcataaccc ctcctctctt ccagtattgt tatattaacc taactttcga tttcctgcaa 44160

taggattttg actatttgtt ctcttcgttt tgctacggta cttgctggtt cgatacctcg 44220

tttcgttaac cacttcttat cttgcttatc tttacagatc gcaatagctt ctttctcaac 44280

ttttgcctgt tcgaaagaaa taccctgctt atcagcgtaa gctaagatag agttacaaga 44340

tttacaaacc atacgtaagt cttccacatt gacataaatc agacgtagaa cgaacatagg 44400

aatgtcatca aaacattgca agttttttgc tgcatggata tgatccactt ggaagtcttt 44460

catacctccg gtgtatccgc acacttcaca agaacaatgc caaacctgtt tcggtaagcc 44520

agtcttctta tctaacatca ccttgccatc tttatccaga cgatccgctt tgaatcgttt 44580

tgcattcaac agtttgattt tgttagggtg tctcatccat gcttgacgaa gacagccacg 44640

aagataggtg taaaactctg cttgtgactt ccagggcgtt ccttcgcccc acattatttt 44700

tggttcttta cttgacaatt ggcaccttct tgattgtgta ttcaccaggt ggaatattgt 44760

gtttaaagaa caacaggcga tccaaacctg ccttgcacat attttctggg atagtgatag 44820

ttactaaaag cttatcccct ttacggttat aaattttata aacctcgcct tctccgtact 44880

gttttgtgtt attcgccatt actaatttgt tccttaagtt ttcggatagc gatcattgca 44940

tcttggtagt aagaacaata catgcacttc aaagcttcgc ggattaaggg atcttttgtg 45000

aagaagtcaa tcagcaacat agcttgatca gttttggcct tacttgtggc tgctgggtag 45060

ttttcctgta ccatgaaact atggttttcc atcatggtag cttccagcgc gatgcaatga 45120

atagctttat ccaagttgat catgcgatca cctttgacac gagtagagta tttagcaagc 45180

ttgcagaaac aaggtgtgcc acctacaaga taagccaact ctataggttg cattaccata 45240

tctgtgtaat ggtgtccgtc aacttgatca ttaaatgcat tagacataat tttcctcctg 45300

gattccgact ccaccttctt cagggtagtc tgtgtaataa gcaacgcgac cacggtacat 45360

aacattaccg ttatcatcaa gaagttcacc attctctaaa acggtgatct ccacatttag 45420

aagatcaggg tctttgtcaa tagaataact catccctaac atttcaaata attcaaaaat 45480

ttcattaccc gttaatgtaa tgctcattca acccccttag taattctgtt ctggagtggt 45540

gttatcccag attttaaagt tttcaagctc tacagaagca tcttcgctaa gagcagcgtt 45600

ataagatgca ttgtttcctt ttgtgcggat caagtttggt ttcttgtgta aagacaaatc 45660

gttagtgaac gccagtagaa ctctgcccgt agtcggggag atattaatca gaccaacata 45720

gtcactgtcg tactgacggc aatcaccttt atcgttcaca gacacaccgt acaggcaacc 45780

tccgataagt acctgttgac ggttatcttt ccctactgtc aaaccagagg aatcctggat 45840

tagttcgtta cggtccagat tatttaaacg taagaactga cgatacaaag cacgggctgc 45900

attgttacga ccagattctg cggcataatt acggaaatca gcagcgttac gaaaaacaat 45960

aatattctta ctcatgatag tctcctcttg tttaaggcgg tatgccttgt tgataaagat 46020

tgtatcgcaa tcagttctct gtgtcaacac ttaaatgaaa aaaggggccg aagccccttt 46080

cttattaaat cccgacaatg gtatttttca tcttatcttt ggaacggctg ttaacacgtc 46140

cacgagaaag tccaccacaa tcttcacaac gaataatatc aaaagaactt actgctgtgt 46200

ggtacttctt atctgccagt acattcacat gtgcactgcc acaacgagag caacgaactt 46260

tggtatcttc gtagtaagca gatacgttag gatgttgcgg aatccatgcc agaatcttaa 46320

tgtatagatc tcgcagagaa actacatcgc catcgttgta agtttgcata cgttcaaacg 46380

catcacgatc accttcacaa caagccttcc acaatgggaa cccttcgtta tcaagttttt 46440

cgctctctgt ttcgaagtaa gcacccatct ctttcagaga attagacggt aagaagaatt 46500

gtttcttagc tgctttcaga gtatcaacca caacatagtt cgacggagga accattccgt 46560

ggtatgcgaa acgctggtta atataagcac ggtcaaattt aacaccgttg tgggctacaa 46620

cgatatcagc ttcgtccaga agcttccaaa tgtcttgaca cagttcccaa tcgtctgtgt 46680

gatcaatatc gaacagaggg tagcttgcca agttcttacc ttcaacttca gcagtacgca 46740

ggtctgcgga agagtaggac agcaagtaac cacggcgaat cacctgatca ggagagataa 46800

aagctttgaa tcgacggaaa gtatacgcaa tttctggtgc tgtttcaacg tcaataattt 46860

taactactgg accttctggt ttaactttag gagttccagc attggcagag ttttcacgaa 46920

ccaagaaatc gttcactgta cttttctttg tcgcgctacc gagaactaat gcagcgatct 46980

ggcgagaaga aagaccttgt gttttaaggc taatgatttc tactttctgc tctgttgtta 47040

atttactcat taattgtctc ctgttgtgaa atgtacgctt gaagcatact ctcaaaagtt 47100

gtgttatcgt tatcccaacg cttcatgtaa acaagttgga atattgtctc gatccattga 47160

tcggttgtta attctactgc ttccccatcc caagatgtaa atttaacacc ttcagggaac 47220

caggtataaa atgtatcttt cactttctgg aaaagttcag cttgggtctt gcattcagat 47280

atcaagttga aacaagaaac atctccaaac ttaataccaa aatcctggta aggacggatg 47340

ctatctgttt tgtctcccat cagcatttga taggccaacc agaaagatcc ccaacccttc 47400

acctcttttt tcttaccctt ttcaacaatc cacaagtctc cgatggactc gtctatcaag 47460

atgatatctg tattcttgaa tacaccgtct tctttgtaga agttaaagag gatgcttgga 47520

gtggataggc tgtctttatc tattgacgcc aggatgtaag agaatttgcc tgtgcgtttg 47580

taatctttgt atccttggaa accgtacttc tctaattcgt catcggcttc gataaccgtc 47640

acaacatccc cgttgtgttg ttccaccaag tagtctttgg tgtcggatag ttgtaagggt 47700

ttaatgtcag atcgttcaga tttatattcg gtaggcatct taaaggcatg tcggaaggtc 47760

ttccctacac caataacccc acaatatttt tctaaattca agtgttttaa gatcgcttga 47820

acttttgtct tagctgcttg aaaagcatgt gacaaagtgt ctggtacgta ttgcacatca 47880

ataatttcaa actcttcttt ggtgaaaggg cgcttacctt gttcttcacg cttggcattt 47940

aatgccccaa gccaaccacc aatttcagtc ttactcttac cccaaaactc tgtcttattg 48000

tcaaattctt tttcggaacc ggaggctgca tgtttagcca gtatgcggcg tttctctaca 48060

gcagaagctg cttggaaagc aacctgatcg tagtcaataa ccacaacagt gtttaggtca 48120

acatacttgt gcaaatcttc cggttttatt aaaatgtacc cgctgataat tccccctatt 48180

attcatcgtc atcatccgta ctgaatgttt ttgtgtaagg acattgccga ctcgcagaga 48240

tctggataac tttgtcgtag atggagttgg aatagaaaca atatttagta ttgccgctaa 48300

cttcctcatc cgaaagaatg agagtagttt gtccagccgc tgccgagaaa cttgttgcca 48360

acagtaatgc tgcaattgtc tttttcataa cacctcctaa aagcctacct cgttgagtag 48420

gtaggcatag tatcacaatg aagtctgcaa gtcaagcact tatttaagaa tagttgcttt 48480

agtcagatct gccatcaaac cacgaatctc gttgaagcaa agtagagtag atgcttcaat 48540

agaaccttcg tcatcgttga agtatttagg gaaacgaagc ttactcaact tcaactcttt 48600

atctactaca aactccagca caccatcgta ctccaagcca atagcgacaa cctctttacc 48660

atcttcttta agttcgattg ctttggaatc gtaaagagat tctttcgtcc agacaacaac 48720

accttcacta tcttccagtt taatatagtt accaagagta aggcgatcag cttcgtgttc 48780

tttcagcaat gtaccaaaac ctttgataat tgcttcttcg tgaacactaa aacctacaga 48840

agggaaagtc cctaaaacat tacggaagaa ggaagtaata gcttccgcta ctttcttact 48900

acagttgatc acatagaaca ggttggttgt tttatccaga accacataat tgaagaaatc 48960

ttccggttcg gtttctggga gaagaccaaa ttcaatatca gctttcaggg ctttcatgtc 49020

ttctttagcg acaaagaatt cttgaccttg gctttccata tctactcgaa tttcttccag 49080

gcgttcattg agaagacgtt taactttctt cttgtcaact ttcttaacag attgaccaac 49140

ctggaggagt tccagatcgg agaactcttt tacgtatttc ccatcatctt caaaacgaac 49200

cagaccaata gtatgggctt gagatccttc agcaggtaca aagtcaatcg gaaattcgtt 49260

gaactcgttc agagacgcta cagaagcatt atcaccagca accatcggaa gtgcagattt 49320

aaaagattta acgaacatat atttcctcct attatttaac tctcacaata accgccccga 49380

agggcggctt acagattatt tcttagcaat gatttttgca atatcaggtt taacaaaggt 49440

agaaggcttc atgatcttac ctttgtcatc aaagaacaca taaactttct gtttttcatt 49500

gtacgtagca tgtacgccag tgtaagcacc ttcaggacga ttggcattac gctcttcaat 49560

cgctgtgcat tcttgtgaaa ttgcaagatc aatatccgaa gtattccata cagccagcag 49620

ttgcttctta gtagggtact tagaatcatt actgtcaaga acttctttaa taatcttttt 49680

gtctgcataa acagaacgca ccagcgtatc acgcgcaagg ttcataatac cagcactcaa 49740

tactgccatc tgaacaggat cagtgaaggt agcttccata ttgtcgaaat cggtatagat 49800

tgtttctgtg aagacagtac caacctgatt ttcaaggaac ttcagagttg cttcttcacc 49860

aaagaacatg tacaccaagt aactggaaac aacaaataca tcggcggctg catccaagag 49920

ttcaacgtga tcattagttt tagcggcttc ttcatactct ttcagttctt cacgaacaaa 49980

cccgatttgg gaaacaaaca tcgcttggtc aaattccggc tgttctccgc cacgcgcttt 50040

tgcattccaa tcgattacat gtttagctgc tttcttatag aagtccacat ctaccttaat 50100

ctctttaaaa aagttctcga tcatcttgtt ctccttatta aaaatagttt tttgcgagtt 50160

cagtcaagta gataacaacc gaacccattt gtgcacctgc tgcacagtat ataagcatcc 50220

gacttaattt tcgccaccaa gtgtttaatg ggaatgtaga agccataata ataccacaag 50280

ccacaataga aagcaacaaa agaatcagaa aaacttcaac acccataaat cccccttaat 50340

taacccattc agccacaaag taaaaacaga aaatgtagat cactgctgcg tacccgatac 50400

ccgccattat cgcatctata gtttttgcta cagagactaa agttgtctta tctttgcaac 50460

agctaaggaa catacagcat acagccaata ctgacaacaa tatatacact gttaaaatca 50520

acatacaacc tcctttattc tgctggctcc caagtccctg ggatcatttt acgaagacgt 50580

acaccgactg gtttagttgg gataccttcg tcagaaaatt cttcatactc aatctcaaca 50640

aactgaccaa tcaaagtctc ggcattctct gctttacggt agttgatagt ttcgtgagca 50700

tcttttcgca tcaagaactc aacaagttta ccattctcca tctcacctgt caacactcca 50760

tcgttatttt tatcaggacg catagaaata actttaactt ccaaagtctg gcgcggtttt 50820

cgtttgatca cgtcatagga acgtttatca aactcataga caccatgagc attacgatac 50880

acgatacctt cagctttttc aagtaaagct ttagcataaa atgcatccaa ctgttcctga 50940

ttttcaatat actctgtatg tactacacag atcagttcat ccagaccact atcttcagct 51000

acagccttaa gagccgccaa tacttcaaca cgcttctcaa atggcatatc tgccacagga 51060

acatcgtaaa catagtagtt gaggagcggc gtattctcat tgtgcttttt ccatgcgctg 51120

ttgatctgtt gcaaactaag aacaccaaga ccagcgaaca cttccccatc cagaggatga 51180

ccaacaactt cgctgatgaa gtttggaata ccttgccagt gcggagggag tttgtaaact 51240

tctccagctt tagacaaaca ttcgcccttc atgttgatca ttgaacgtaa gccattcagt 51300

ttgtattgtg catacccagg gaagaccact ttatgtgcga aatctttata gtcctggcat 51360

ttcataggtg tggatgggac gtggttctcc gcctcttcca tagtcttata ataaccattc 51420

ttaagttgct tcgtccactt ggcaaaagct tcagcttcgg cttgttcttc tggagtggtt 51480

gcatttgcac gtccagtgtt tttagcttct gcctcatacg atttttgtgt aatcttccca 51540

ccaactttac cgtgagctac agtgactgtt gcaccgtccg taaagacggt ccattgtttg 51600

agtttaccat tggagtcttt accgtaaagt gtctgattag tctgtttcaa attccttcct 51660

ccttaataat atggtgtagt tcgaaaataa catcttccat aacaacgttt tgccagtctg 51720

tagactctgc gaaatactcg tctatatggt gaataccttc gctcttaaga tgatcgatta 51780

tcatccataa gaaatcttct gctggtgtac ctttttgaaa ggtaatggaa gtctttttct 51840

cactaccttc gaaatagaat gttgttgcca ttatatttcg tcccaagtaa ataatgcaat 51900

ttttaaccga ttgcagatct catccaaatc ttgatccaag attatggttg aagttgccaa 51960

catatgatca acactcgtaa agcgttgacc tttggcataa tccaacatgt cttcatacat 52020

ttcttcaaga tcgcaatcgt cgtcgaaaga agcaaggaaa tcttcatcct cgttacgtac 52080

ataaaatgca gtactcattc ttcttcatcc caagattcaa aagcacagca gtcatcggtt 52140

tctatagtac cagtacgtcc acaagaaagg caagtgattt tatcaccgta gaatacataa 52200

ttctcgtctc cgttggtatt cacggaaatc tgatcactgc cacagggaca ttccaaccaa 52260

ggcacaatga tatctttctt catgttttct ccttaacctt taggtgtgca gaatccacga 52320

tcaaatacat tcttacggat gccttctgct aaagccatct cacaagcccc ttgcgatccg 52380

aattcttgtg tataatcatt actaccatga ctccaaagag caaacaaaat atagattgtt 52440

gccatttttc ctccttaagc attcagttcg ttgttgagtt cgtaaatctc ttcctggaga 52500

gcatcaatct cttcttcaag acgaagattt tcttctttaa gctgtaggat ttcaaagttt 52560

ttctcttcaa tctcgttttc aaacttcacc ttattcaatt cattttcgta atgaagttcg 52620

gtgtagtcgt cacgcatctg ttcaacaaga tgaatcagag ataagatata gttatcccca 52680

gtttcatctc gccactcttc cccgtggcgc aagattgtga ttcgtccagt atcttcgtca 52740

agaatcgttt cgtacttacg accatctgtg taaatttgca ttgttcctcc tttggttaaa 52800

tgccttcgcc aacatttctg tttgaccaca gtttcaaagt ttcagcaaca atgtactctg 52860

tgatataggc taacgcttcg tgattttcgt aataaacctt aacacccaca tggtcaagaa 52920

tatcccacgc ggtgtgaatc gcttcgtgtg tcacgagttc tggtgtcggt tcggcaatgt 52980

ataacataca cggacctaca ctttcaattg ttgcaaatgc ctttgttacc gaaaaatttt 53040

tagttttacg gtgagggaag tcttccagat attcttccag actgaagtaa acccaaatgt 53100

ttgcatcaaa aacatcaatt ctaatttttt ccattagtgc tccctttatc ggataatttt 53160

gtgatgatca acagtgataa cttcacgttt ttccatcatc tcaataactt catcaaagtg 53220

caggatcttg ccatgtgcat cccaaccaac atcaagcatc ttaccgtagg cttcatcagg 53280

gaaagatccg tgagaatgtc cgtggagcat tatgctaccg cgaccctgtt tgttccagga 53340

ggcaataggg taatgactta agacgaagaa cagcttctgg tatttcactt cgtcataata 53400

caccacttcg ccatactctg caagcacttc tcggtgcttg ctgtaatcat ggttcccaac 53460

aagataagtg atatctcctt ggagacggga agcaatctta cgggtatctt cttcagtccc 53520

gaaacagaag tcgcccaaat gccacatctt aacaccaaga gtagtagcac atttgttcca 53580

tgcttcgatc agggcttctg tcatgtggtc catatcttcc cacggacgat attcaggagc 53640

aaactctcga atacgtttgt gcataaaatg caaatcgctt gtaataaaca ttagtataat 53700

ccctcgctaa atagttcatg gtcgtgacca ttacgctcat ggtcaatctg gtcagcattt 53760

tccgcgatga tgtaagcatc ctcacgactc caccaattgt aatactgatc gataaagcct 53820

tgattatctg gcgtgcacaa agttttgctg atcacacctg cttctttcaa cacgcttaac 53880

tgtgcatata gtacaggatt tgcatgtcga acgcaaggga taattaactc ttcaccatta 53940

ttcagaatat acttattcgc agcacagacg ataacacgtt gcgcataatg accctcacca 54000

atccaggtga gaggttggta acaaatgtta tgatcaagac tgctcattag gtttctcctt 54060

actcttcgtc tgtgtgtaca tcgtaaaagc aatcttcaaa agttgcaagt gctttttcta 54120

cgtcaggcca atggttagtg tgtttgtata cataaaaccc atccccttca tctacccaaa 54180

taacataaag cataacacct ccttaatagt tcacacgaat catttcaata ccatacctca 54240

acatagattc ctctgcaagc ataaacttct ctgccgctgc tgggttagaa gtatgtacaa 54300

aaataagagg attgccaaaa caaggcttac cgaaaaacag aagttcttcc aagcgcaaga 54360

aaacatcgta cccctcttga tcagagtctt ctcctaaatc attgtcaaaa tgccactcgt 54420

ctacagaacg ataacccttc tgcacctcat tcatgtaacg agaggcaaga agttcctgtg 54480

atgttttaaa ccagattgca tctggacaac cgtaatcttt cggatcacgt aaatcgtcaa 54540

gccaaataat agcacccata taacctcctc taagctggca tgattaaaaa ggaactctcc 54600

acttcgaaat gccaatcatc cacaagacct gattcccaat ccttctcttc gtggaaagag 54660

cagatccaac gatacatacc tggcggtgta ttgtcaacat cttccggcaa cccgtaatcc 54720

aaagctgtct taccgttctc tgtgaaacca gggtggagat taggagcttt cagtacagaa 54780

accatacctc caacatccac agcaacaacc cactctgtca tgtatgggct gtcatattga 54840

ggttcatcgt aacctacctc tacagcttcg tcacgcaaca cgataatagg actaccacca 54900

caaggcggaa caatcatata tccaatgtct acaccatcag actctgttac aatatctaca 54960

acatcaaact cgtctccgtt tttaatatcg tagcttttat tcaaagaaaa gaattgttgg 55020

ttatctacct ctgctttagt aatttttatt ttcatttcac tttaacctca aattcaacag 55080

gttgagcctc aatgtaaaaa cactgatact ctgctttatc accctcatca cgaggccatg 55140

ccaccagaga gttataacaa tcttctggtg ctttgaagac tgttttagtc tccacggtga 55200

cattcccatt gagggttgcc agtacaaccc atccagccac actgatcata gtgcctccac 55260

tttccaagtt ttaccattat caaccaaagt ctttgcaaca gcatactctg ttccgttaac 55320

aaggaagcag tataccacga aagactttcc aattgtaacc gtcttagtaa cctctacacg 55380

cttaacacct tgcatgtatt ttgccttgcc gaaatagagc agaatcattt tgcatccttc 55440

ttgttgttga taacatccag ggcagtgtat ggttgattcg tgccgagata ttccaggact 55500

aatggagaac cttcttgcac cacttcaaag ataccgtaga cagagctttc agcaaatacc 55560

atttgctccg caccttcgtc gtttacaaca agagaaaact tcttacctgt cccgttgagg 55620

atctcgtact tcttgcctac agtaaagagt ccagagtacc attcgttctc ttttgctttt 55680

acacacttca ggaaagtctt ggtcatttct ttttaccccc tttcttggat ttctcgtata 55740

agcctaacac agcagcttct tttgcacaag taggaactga agtcattcct cgtctttttg 55800

cgtctggcat ttgcaaggtc catactcccc accagtagag ttggtcttgg gtgagtggtt 55860

cagccattgg gtctgcctcc tgctcctcaa gacgctgcaa cgcttcttca attttctcga 55920

tagctgtgtc aaagacgaat gcctccgtat caaatttatc tttcttaggg actgatttag 55980

caaccttcag aacttcgttc ttcttgggtt tcttagtagc ggtttttgtt tttgatgact 56040

tcttcggtgt tgtcgccatt aactacctcc atacgatcta aaaaatcacc ctggaaacgc 56100

cagtagtgct gtcctgtacg taagtcttca tagatcacaa ttggatcgaa gactttagat 56160

actccagcag gttttggaaa ttccacaacc gaatacaaac cacctttacc tacgcacgag 56220

taggtcacac ctgtaattgg atatttactc atttcttcgt gtactccatc atcggcatgt 56280

ggtcgccagt gagcgtaaca cggctgtagg agacattttg tgtcttcaat gtctcaatcc 56340

attgcaagaa ctcttctttc gcttctgtgt agttctgcgt gcctttaact acatggatat 56400

catccagggt gacacggaaa atcatttgaa taacatcttt cattgctttt ctcctttgtt 56460

acgtttgtgg atttgattta tagtgttgca cacgttgcag acaatgttgt ctaaaacatt 56520

gattgccacc aggactataa tcaatgtcag gatagggtgc tccgttatcc aactaagcat 56580

tttttattac ctccaattgt ctttcaaggt taactctctt ggagaactga taatggcaca 56640

gcttgcaatc aatgtcaaca acctgatcat gatttaatcc gacaatctcg acttgcttac 56700

caccagacca atctacgtat tcgaagttct cgtaagacga acacttgctc aacaggaaag 56760

caagctgttc ccactccact gaaatatcat gtgcatgata ggaggaacaa aatacgtccc 56820

catcataaaa tgcatgaatt attgcaccat cttcaaagcc gctaacaatc cacttcgtaa 56880

acctcatcct accctcctaa atgcgtctct aacgcaatat ttaaatgaag atgataaatc 56940

accttacctc acaccttaat caatccacaa tgcgatctat gaagtcttcc atatcgctat 57000

acccataaca cagacggtcg aactcttttg gactatctac ttcacgacta ccgactatct 57060

gatcaaccca gtaagttttt gcatgtgtta catagtgacc tccaggttta ggtcgtggtg 57120

ttaggttgat accttctttc atacgttgct tggctgtctt agcaatactc attctattcc 57180

tcctctcttg tttaagttta ttcactataa cagatacacc aaagaacaca agcttttgca 57240

caatgaaaat aatttgaaaa ttgtcttgac tttgattatc gaaatgtgat accctaaaaa 57300

cctattaacg agcgcagcga gttaaggtct tgtttttatt ggttaagtat atgctcgatc 57360

taaaagactt aggttttaaa gatcttaaag acagttagat agtaacagat ctgctctata 57420

ctgatcaaca aactaactaa caaaaaagaa aataattatt attagttagt agttaaaaga 57480

attagttgac ttttagatct tacatatgtt agagttcatc ccaagaaaac gagaaccgag 57540

gaggttatat gagtaccagt gagaatccgt gcaagtggca ccacatccca atcaaagatt 57600

gtatgtcttg taatccgaag tcaatttaca acatttcatc ttacgatgaa gaagataact 57660

tgatggacag tgatgagggt tggggagatc ttgagtggac aggttacgaa acaaaacgtg 57720

ataagattat tcccagttga cttaacataa ccttcgtgct acactaaaag tgaggagatg 57780

ccgattactc cccattttgg ggttcgaagg atttatgcaa aacaattttt attttcgttg 57840

tcgggcttgt aacagacact tgactaacca ttccagacgt gataaaaatg gaattattca 57900

agagtcagat ctctgttcga tatgtacagg cttggcattt gacgacagcc ccgaaagggt 57960

ctatcagcat caaggtataa cagacgttct catttctaat gtaatggagg acgaacaaac 58020

aatcgattag gaggaatcaa tatgtcttta gtagaaaaac atgtgttgac tgatggtgaa 58080

ggtggagcaa tcttcatgtt caacccagag aagtatgtta ccgagagtga agacggtgtt 58140

aggatcgtca tcaacgagga cgttaaagtt atcgatttcc tggctcttat gcgtcaaatg 58200

gatctggaga actcttggca aaatgctgaa gcacttgtta tagcactact tgaaaaacgt 58260

ggctccgaag aggttcaaga ttacgcctaa aatatttaaa ataagtattg acaagcgtgt 58320

cttattttgt taagatacgc tttatcaagt gtggtaacag ggtcgttgac tgttgttaag 58380

acggatttgc atcggggaga tgttagaggc tacttgttca gaggttagct accaaagaac 58440

atacggaaaa gcttcggctc aagaacattt acgctgaatg ttgggcttac gtccgaggaa 58500

tggtagctac ttcctactac tttcaacgtc tctgttacca catttacggc cctgtagctc 58560

aatgggagag cagtcggctc ataaccgata ggcaactgga tcgaaaccag tcagggccac 58620

caacgtcttt atggtgtaat ggatagcaca ggagtcttct aaactcttag tcaaggttcg 58680

attccttgta aagacaccag aacattttag tatgaattag ctcaagtgat ttgaacccta 58740

ccactgatca tggttggcag agaatgaggc aaatatgcat gagcaagcta acggtgtaca 58800

acgtttgatt gcaagggtag attgctcgaa gagtaccgtt cgtggagaac gggggttggt 58860

ggagtcacca ttcataataa aatgttttaa aggtgttgac aatcaacatc gttctgtgtt 58920

agattaagtg tatggaaggg caaatcgatt ggcgacgaaa actgtcttga aaacagccga 58980

gcttgaaaga gccttgggag ttcgactctc cctccttccg ccagattagg tagattggtg 59040

aaatggtagc cacgacagtt tgctaaactg tggtcggaaa cggcgtgtag gttcgagtcc 59100

tacatctacc gccaagttgt ttttaatgta gcttgttgtt cttacttaag aagagtaaac 59160

tttcggcaga tggctctcac cgtcaccgat accacatagt cagtaccgcg aatactggct 59220

actttaaaaa caattttgtg agattggcag agtggtcgat tgcgggggac tgtaaatccc 59280

ttctgaaagg cgcggtggtt cgaatccatc atctcacacc aaatttaaac tggaggttgt 59340

aatgttattt gataattggt ttgatcttga gaaagtttgt attggaatta cattcacagg 59400

cggtgctcca gaatataaat acgctatgtt acaaactaac ggtttgtctg taagacttca 59460

aagtatttaa gaattatgga cttgtagagt taaggtaaac ccccagtcgc caagaggctt 59520

ctggaattgc aggtatcgaa tcctgccgag tcccagcatt gctcctatcg tataatggct 59580

attactgcgg ctttgtaacc cgcaaatcat cgttcgattc ggtgtgggag caccaacaca 59640

acattaagag cactgtgatt gatcgtcgta tcctgtgcac aaggtctgct cgaaagacag 59700

tgttcttagt attatgttga tcacaaaaag cgtcacctga aacttgaccg gagtagggtt 59760

ggatggggta aagaagaagc ggattctccc atcgacgtga gagttttcca gtgttgcaag 59820

cgacacccag gagattaatc acaaccgaac gcagggttag tcacccaacg ggatactcgt 59880

cactgtccca ccccaataaa actattgaca actgatagta aaatgtagta tagtaaagat 59940

gtagggcgtt cttggtgtag cggtagcatt ccgtccttcc aagtcggcgg cacgagttca 60000

atcctcgtag aacgctccaa attatttaat aaagggttgc cagaaaactt tctcttttac 60060

tcatggtttt ctccttctct cacacctctt ctggttgccc ttcattaaat ggtttgtagt 60120

gtagtccggt agcacacgac actttgactg tcgtaggttt ggttcaaatc cagacagacc 60180

agccatataa cggaatttag ctcagtttgg tagagcatac gctttgggag cgtagggtcg 60240

tagggtcgca ggttcgaatc ctgcaattcc gaccaattaa atgtttcgta gttcaatggt 60300

agaacccacg actgataatc gtgagataca agttcgattc ttgtcgaaac aaccagtttc 60360

ccgttagact tacaggataa gagatccaca actattgtga tgctggttcg aatccagcac 60420

gggacaccta atagcgggat agaggagttt ggtcgtcctc gccagtttca tatgctggag 60480

atcatcggtt cgaatccgat tcccgcctcc aaatatcgcg ccagcggtat ctttgaagac 60540

cttgcctggt tccttctaag agggaggggt gcgactccta aaacgcacaa cccactaaac 60600

actaaatact taataggaca catcatgtat acgttaaaaa caatccgtaa attttctaaa 60660

gaagatgaag taaagattta caataccgtc cccttcaatg taaatgaaga agaattcttt 60720

tactgcttag aggatgacgt aattattcag tatcattggg aaggttatga tcgaccagat 60780

ggcgtggtgg cctctgttat cgcaggtgaa cgtgttatct ttgttcacaa aactgaatca 60840

gcctatctga tgaacgacaa aggacaaact gttaaggtaa tcaaccgacc ttaattgaga 60900

gttatcctaa agacattttt ataccaaaat gttaaaatgg cattagggac acatgttaat 60960

ttacacccta acattcatag agcctccctc gtggaggctt ttttattttg gagataccca 61020

tgaaaataat tgcccctggt ataggaacga atgcatggac taacattttc gacatagttc 61080

aagtagatcc taaatatgca catattatag agaacgtatc ctctgtcggt atacgtttag 61140

ctttaaacaa tgaaacactc tctgaagtta aaaactgcat cttattacaa cctaatgaag 61200

attttttcat ccagcccaac tggggcgttg cctctgtaat agcagagggt acagcaccag 61260

gtaagatttc gatacgtagg gcagaaagca ctgatataaa agctgcgatg cgtaaaattg 61320

taggtttgtc agatccaaga gtccttacac aaacattgac acaaatagaa gctgcaagta 61380

tagaaggtag actttttcaa cttacggtag atgttatttt accagcagga gtgacacagt 61440

ggtttgagtt tatagctcct acagataaag agtgtgcttt tacagattgg aattttgctc 61500

ctttctatac aggttttgag gctaaaatta tcacaggttc tacgggaggt gtagtcggta 61560

cttcttacac taacaggaac gttaacttaa aatatggaac aaattccact gcgcaattcc 61620

gatccttaac tggaaaccct acggtgggta ctgtgtactt catacctgca tttatcccag 61680

cctctggtgc aaatccgaac aacagggcaa caggggttag ttcagtatct tctggttaca 61740

ggataatacc agcaggagga tcttttagga tggaactgat aaatacatct ggaaatgcaa 61800

acagggtggt tatctccctt acttggttag aagcccctac ttctgtactg tcttaattat 61860

aaaccttgac actatccttt ttatatgata ggataaaatg taggaggact gtatatgcct 61920

aaactcaaac gtggtcgtgg acgacctaca gcagaaatgc aacaaattta tgctatggat 61980

gcagcacaag caacagtgaa ggaattgttt gccgagaact atctcgcagc aatgaaatac 62040

atcgttgaac ttgtgtatga taacgaagca gcaaaacctc tccgattcca agcagcgaaa 62100

gccattaaag agcaagtaga agactggttg gaagaacatt atgaagcaat ggaagaagaa 62160

actcctgccg ttgtggaaga acagcctaag aaaattgtaa tttaacctct cctctcttgg 62220

ggcactttgc cctctcctcc ttgttatttt aacaacccct ttccgaaagg ttaggggctt 62280

ttttcttttt agggcttgca ttgtgtatat gttttgtgat aagatcaata ttgaggagga 62340

taaaatatgc gcaactttgt agttaagaat gattttaaca aagcttctgt ccatcgtgac 62400

aggaaaaatg attactcccg tcaatgggat cttgaggacg aattagatga tgatcgagaa 62460

gacgtgggaa gagattcttg cggaagccaa accgatggaa gtgaagaagg tagtgaataa 62520

ctctccatcc ttacctaaag tgagatttaa agatgacact gataacaatg attctgaaga 62580

gtaagtggtt ttacgttatt cttctgttag ttgcactttt agctggtttg tacttcggcg 62640

gaaaggctct gtatgataat ggttacgcag caggagaaca aaaccaagta actatctatc 62700

agcaacaaca agcaaaagct aaacaagact tcgatgttct tcaaaagaaa gccgatgatg 62760

aacgtctggc acttaacaac caaatagctt ccctctccga aaagaatgct aaattgaaaa 62820

aagatttagc aaacaaggag aagaaggtga atgaggagaa aacaaattat gcaaaaacta 62880

ctactgggtc tatgtcttgc ttcggcccta atgatgacgg gttgcacatc ctcaacaaaa 62940

gcctccccga ttctaattga cgaatgcaaa gctgtagcag tgcaggaagt tagaggacca 63000

gactcttctt taatggaagg tgtaagtgaa cctgtaccgt acacagacca acaaattgct 63060

aaaggtgttt ctcgttctga agtagttgat aacgggacca caaacaatgt tctctggagt 63120

caagatcgta ataaagtaat cggtctgcaa aactacatta aggctctgca agagaaaggg 63180

attatcagta agtaaggagg agaagtggct aagaaaaaag cggctggcag tttagctggc 63240

gctgatatcg aagtaaaagc tcaaccaggt aaacagacgc tggcaatgga tctaatgcca 63300

gaagttatga tttacggtgg tgccgcaggt tgtgtatctg ctgaaacaga gtatcttacc 63360

cgtgacggct ggaaacaaat aagccaatac agcggagaag atatctatca gtataatcct 63420

aagagaaaac gcttagaatt ggtcacgcct tacttcgtag aatatgatat gaaagatgat 63480

aacttgtacc gaatcactaa cggtgcagga gtgttccaag aattgagtat ggaacatcgt 63540

ttcgtctttt acaagaaaaa gaaatccaaa aaacattttg agattgctgt gggagatctt 63600

attgctctgc aacaagcagg ggcaccactg aaaggataca ttgaagatcc gttcggtaag 63660

cgcgttccat ttaacatccc aggcaaacaa aacacttatt taaaaataca cgaggttatt 63720

acatccgatg ataaaaaata ttgctttgaa actccaagta gctatattgt ccttagacgg 63780

ggcaatcact gcttcgttac tggcaatagc ggcaagtctc gtctgctact gctaaaagct 63840

cttaagtttg cctataaaga tcctttgttc ggtggtatct tattccgtaa gaacaccact 63900

gcacaccgta agccaggtgg tttgtttaca gaagctaaac gtctgtacct gcctttacaa 63960

ccgcacgtaa aagaaagtac gatggacatt atctttgagg ccactaacgg tggtactttg 64020

aagtttgacc atctggagaa cgataacgtt acagccgaga ctaaccatca gggtacacag 64080

tacagtatgg ttggttttga cgaactcacc catttcaccc agtacgagat gctttatctt 64140

ctcggtcgta tgcgttccga gtcagaaaca tctttcatgc tttgtacctg taaccccgat 64200

gcggatagct gggtactgaa ctgggtgtta ccttatcttg acgaaggtgg ttatcctcgt 64260

gaagatatgt gtggtaaaca aacctacttc attattgtaa atgatgaacc tgtatttgcc 64320

gacacagcag aggaacttaa agagaagtat cctgaaaact gtttcatgga gaacccaaac 64380

actggtgagg ttgttacgat cgaacctcaa acgtttgtgt ttatcggtgg tacgatcttt 64440

gataaccctg aactgattcg ccttaacccg aaatacctgg cttctcttaa atcgcagaca 64500

gcgatcaagc gagcacagct tctggatggt tgttggtttg cccgtattca agaggaatca 64560

ttcttccaac gtgaatggct ccacaagata gaatacaaag atgttcctaa caacatgaag 64620

tatatgcgtg catgggataa agctgtatcc gttccaagtg aacgttaccg ttatcccgac 64680

tatacggcat ctgttaagat gggtaaagat ctaaacggga acatttatat cttcggggat 64740

tatgattacg acgctataga cgagaagagt aaagttttcg gaagattccg acgcttacct 64800

ggtgacaggg ataacctaat cctccaacag gctaagattg atggaccaga tgttcagatc 64860

gttcttccaa aagacccgtc tggtgctgga ctcattgaat atacggaatc agctaagaaa 64920

cttattgttg aaggcttcgt tgttaaaccg gatgcaatgc caggtaacaa aaagaaactc 64980

caacgtttca tgccattcag tagtgcctgt cagaacggct ttgtttatat cgtagaagat 65040

tcatttccta acattgaaac tttgaatcac ttttataaag aattggaatc ttttaacggt 65100

gaacgatcta cagcaatgct taaagatgac cttccagatg ccgcagcatc ttgcttcaac 65160

actctggcga aagagacggc acacaaggct gtagcgatac ctgcaacaac aaatgcacct 65220

actggataca gtaacatgaa ctccaggcat ttcactaccg taagtactcc tcgtcgaaga 65280

taattgttga caaaagttac tttaaagtgt acaatgactt tatgggcggg ttaatttatt 65340

taacccttgc cctattgatc tttaagcaca gtgccggagg aagaaagtgt caacagaaaa 65400

aagacagtac actaagaagt cggattactg gaaacaaagt gcagtgaaca aagctgctct 65460

ctctccgtca aaacaggcag agaaagaaaa gagtttactt ctctctcctg aaatcgggac 65520

gattgggttg aactcaatca aagcattcac taacttcatg caaccgtatg aaacacgctt 65580

tcctgaaaac atccgtactt ataaggaaat gggtgaagac ccagatgttg ctaccgctct 65640

tgacgcaaca tacattttcg tagacagagc atttttcgac tttaaaataa aatacaacgt 65700

aaactctgcc aagtcaagaa aagctgcaaa gtttattgaa tatatgttac gcaacatgca 65760

agcaccatta cgtcagtatg tgagatctct tcttacttac aaacagttcg gttttgcatt 65820

tgctgaaaag gtgtttgaac ttgacgaaga tccgaagagt ccatactatg gctattaccg 65880

cctcactaaa cttgctttcc gaccgcaaga tactttggat atggcaaacc ctttcaccta 65940

ttctgatgat ggtcgcaccg ttatttcggt aaaccagaac atcactaata ccatgttggc 66000

tccaggtaac agtaagttgc aactgggtgt acgtcaaatc cctatgaaca aggttatttt 66060

tgtgggtagc aatatcacag aaaacaatcc tttgggtgta tcacctctgt tggctgttta 66120

tcgtagctgg cgtgaaaaat ctttaattca agaattcgaa gttatcgggg tttctaaaga 66180

tttgggcggt atgcctgttc tgaaagtacc gagcgatatt cttaaccgcg catctcttga 66240

tcctgccgga gatgaagcac agtccttacg agttctacag gcaaacattg ccaacctgca 66300

ctctggtgag caagcataca tggtattacc ttcagacgtt tatgaaggaa ctgttatgcg 66360

tcagtacgat ctggtgttcc agggcgtaga aggtgctggt aaacagtttg atacgcaaga 66420

actgatcaaa caacgtaaac tggatattta caaccgtttt ggtgcgggtg tattgataat 66480

gggtgacggg gacgctggca gttttgcatt gtccgataat aaacagaccc tgctctcaca 66540

cttcattgaa cgcgatgtag atattgttgt tgaagctatt aactctcaac tcattccaca 66600

agcacttcgc ttaaacaata tcttcctttc tgatgaagat atgccgaagt ttgttagtgg 66660

tgaaatgggc gatcctgata ttgaagtaaa cgctaaagcg atccaacaga ttgttgcagc 66720

aggagctatt ccacttacag cggaaattat caacgagttc ttagagaagt taggctttga 66780

ttatcgtata ccagacgaca ttgtttctga caaggataaa ttcgtagaat atatggagac 66840

attcatgcca gataaaactt cacgttctgg ggacggtatg gcgaccgcag gtacaggtac 66900

atccaccagc gtttcggctc ttgatccatc ggcagcaaac ttatcgaatt aaaaaattaa 66960

acttttttga ttttaggtat tgacaaaatg acgaatatcc tgtagactaa gatttatggg 67020

atatttgttt ttaagaggtt catatggaac ttaataaaga tacactctgg gaactcttca 67080

aatcgttcag cggtatgaaa gaatcaaccc catcccaaca agattctaca cccctcaaca 67140

acctcaaaca gtaatcaaac ataataagtt tgacgaagag caaatgcagg ttattgaggt 67200

attgtattgt ccacctgaaa aagacgacct ccacggtgag cgtatgtctg atatggaaat 67260

caggaagatg gtagacaact tcaatgagaa tattactaac gtaagtggta atcttggtca 67320

catgaagaac acagacaaat ttgccccagt taaagcttgg gtgaacgaag ttgattgtta 67380

cattggtgac gaactcgttg tggaaggaac tcccctcgtt aaaatccaat tcaacgaccc 67440

tgaactgtat caagctcgta aggacggtgt tcttaaaggt ttgagtatag gtgcgatggg 67500

cgtgaaagtc aagaaggact aattgtggca aatacatatc ttactaacgt agacttttcg 67560

ggtaaagcta cggaagaaac tctgggcgca catatcgcat acactttcga ttttcaaggt 67620

ggtgcagcgt ctggttacaa taccccactg ctttttaaat ctgacggctc tcctgaagtg 67680

accttcgaga aaatcgaagc tctggctaag atgggtgaag acgttactga attacgtaaa 67740

ggttacattc agcaagtaat ggaccgcatc gccaacgctg taaacgaaaa gttctccaca 67800

tgttgggact gggtttggct ggcagatgta gactttgatg ccaacgttgc aatcttctgt 67860

tctgatcacg gtatgtttgc cgtaaacttt actttgaacg gtttagttgt tgaactcgaa 67920

gaagttgcta aacctgttgt acgtactacc gattatcaag ttgttgatgg tgacgttatg 67980

gtttccgtcg atttctttga agacctgatc gaagatgctc ttggcgatct tattaaaggt 68040

gctctgaaac acgatcaagt taaagattat ctggtgaaag cacacaatgc tcaaaccaat 68100

aagaactctg tggatgcaga ggctaacccc gcaggtgaca aatctgcaaa cacaactgga 68160

atcaataaag gagaattacc tttggaaaat attaacaaag aagaattcct gaaatctgcc 68220

gaattccaag acctgatgaa agcacagatt gctgaagctg tagaaaaagc agcgacagaa 68280

gcccgtgaac aagcagaact ggcagcacag gaaaagatcg ctaaagcaga actcgcagca 68340

gaagaacttc gtaaagctga actggctcgt atcgaagaag agtacaccac tgttgttaag 68400

tcctacgctt tcgtagaaga agaaaaagtt gaagctctgg ttaaatacct gatcgataat 68460

aaagagatcg ctgaaactgt tgttaacgca ttcgaaaaag cccgtgcaga agttgaagca 68520

gttaagaaag agtttggtaa agaacaaggc gctgatgttg ccaatgttga agttgttgcc 68580

aagtctgcat ctgaactgat tcgtcagaaa gctgccgaac tcaaaaaagc ccagaaacaa 68640

taatcttagg agattaaatt aaatggctaa aggaattact ttatctaact ctcgccagga 68700

attcggacac ctggttctgg gtggagtttt tagctctgac ctcggacact gtgtacgcga 68760

agtaaacctg gttaaaaccg caaccatgaa aattggttcc gtactgaaag ctgacaacac 68820

tgaagctgct gctgctgctg acgctgctaa agttctgctg tggactgacg ctctgttcgg 68880

tatcgatgat gttgcagttg gtgacacttt caccgccgtt gttggtgtac gcgacctgac 68940

tctgaaccgc tttgctgtat tctatagcaa cggtaatctg attgacgacg ctggcgtagc 69000

agcactggaa actcgtgatc tgaaactgac tgaaaaagtc gttttcactt cttaattttt 69060

gataggagat taatataatg ccagcagtaa ttttagatca ggttgttgat ttcagcccaa 69120

tgatcgaact tcaggatact cctgatacac tgattgccag cctgggtctg tttgatgtac 69180

agtaccatgc aactaccgtt gttgaaatcg gcaaacaaaa aactcaagat ggtctgatcc 69240

ctgcccgtga acgtggtggt gagcgtaact tcctgactat ggatactcca gaagtcaaag 69300

tgttccgtat cccattcttc ccactggata agaacatcaa agctcaagat atccagtctt 69360

tccgtagctt cgctatggct ggtatcaacg actctctgcg tactgaagca gaagtagtta 69420

accgttatat gacgcagatc ctgcgtgatg ctgcaaaaac caaagagaag atctttgctg 69480

aagcagtaat gggtcgtgca tacactggcg gcggcgctgc taactctgcg tacaactggt 69540

acaccgagtg gaacgttacc cagaaatcag ttcctgtaga ctttgcttct accactgttt 69600

ctccagcggc taccgttgag caagaagctc gtgcatacat catcgacgaa aaacaagacg 69660

gttctaccgc tactcgtatc gttgctctgt gctctcgtga gttcttcgct tctctggtta 69720

actctccgtt tgttcgccag gcttaccagt acttccaggg cactcctaac ctcctgcgtg 69780

accgtctgaa cggtaacatg gacgttcagg tgttcgaatg gaacggcgta acctacatcg 69840

aagatattca cggtaacatc cctgctggtg aagcatatgt tctgcctatg ggtatcgcgg 69900

atatgttcca ggcgcactac gctcctgcgg atactccaga actggctaac accgttgctc 69960

gtgaactgta tacgttcatg atcagtgagc accgtactgt aagtcttcag tctgaattct 70020

ctcttctggc agttaacgtt cgcccagaac tgattgttcg cctgaccact gcttaataga 70080

actctgttct aaaagaggct cgtccgtgat gggcgagcct tttttgttat atgaacacta 70140

tacaccgagg agattattat gtcaaaccca caattaaaac gttacttcag ctaccacgaa 70200

atgattcagg acttctctaa gctcgtgagc aatctggatg cagagaagac tctcacccaa 70260

gcacctccaa tgtctttcta tctgaaagat ggtcgtgttg ctttaggtgg taattttatt 70320

catttcttac aacgtctgcg tgaagagact ggtctgttgg ttcatccgtt tagtagcgaa 70380

gagcatatgg gctatttcct tgttagcttc gaagattaca aagagatcga agtcgaacaa 70440

gaagttctga aagttgaagc agtagattca ccagccaaga aacgatcact ccgtaaaaag 70500

taaggagaag ttatgctgat tgctactgaa ttggcaccaa tggttcgaat ccttgtctat 70560

aatccatctc aagaaacttt gccagacgaa gttctgattc agattatcca gacgtggatc 70620

gatattattg gtaacgccga ctctaataag tgtgctgttc tgtggaacag tttaatttct 70680

gttcttgagt acctgtggaa cactgatgtt ttaaataaca acagtcaaag tggtggtgca 70740

atgtcccgcc gtgaaaagat gggcgaagtg gaagtagaag ttaaatttag taacggtcaa 70800

gtagaataca aatctccttg ggaagatatc tacaacaact acctgaacgg cttaatggtt 70860

attccaggat gtactgttgg acgtggtgca acaagtcgtg ttctgattgg tggtgtagat 70920

gctcgtgaaa tcgatcgtgt caacagtgat cctaactctg tgaatggttt aggtaatgtt 70980

gccagtgtag atcgtaaaac acgtaacgta aactatctgc gaaactacgg acctgtatca 71040

tattatcgta gggacaaata atgaaaagtg agatcttacc aggcacagac acagactggg 71100

atacaatcat taaaaagatg atggtattag agaatgtgga aattgaagct ggtttccttg 71160

aaaacaagag acaccctgaa tcagatctca ctattccagc gatagctgct atccagcaat 71220

acggtaacga aacaaataat attccagccc gtccgtttat gactgacggt gctgtgattt 71280

ccagcaaaga gatagccaag ctgttaccga atgtttttgt tcaatacctg atgcgaaata 71340

aaggactcgg agtttttgag ccgatagcga aagcttctcg tgaaggaata gcaagagcaa 71400

tagcttccca acgatataga gcactttctc cggtgacaat taagattcgt cgtgacagag 71460

ggaattattc tacgcacacg ttgattgaca caggacacat gattaacgag atagactcga 71520

agatcacaaa aggcaagtct aaaaaataac ttgcataaat tttgtttttg atgtatacta 71580

aaaaggagat acaggtgtta cttgatcaat tcactctcct tgatcttacc tcatatcaag 71640

ggcgtagacg tgtctacaaa gaagctgtag gttctgttat cgccaatatg ggtagtaccg 71700

tagaatatga agagtttgag atagcggctg caagtctgca accaatctct ggaaaaactc 71760

taaaagcact gcccgaaggg tacagatcaa aggcgcaata tagtttctgg actaaaacag 71820

aaatgcgagg catccaacaa ggaacctctc aactatccga ccaaatcttg attgatggtc 71880

agtggtattc tgtgtactcc cttaaagatt ggactcgaac tactttccta acccatcatc 71940

attgtgtcgc catattagac gatcaaaaca atacatggga ttacgatcaa gagggaggaa 72000

actttggcta atatctacga acaaatcgaa gcgtatgaga atgctatcta tcttaacata 72060

ggtagtttcc ttcaagaatt aactggattg ccaatttacg tgatggacaa accgttcgtc 72120

caaccaacca atccatacct ggcactacgt attatcacct ccagtgattc tggcgggtgg 72180

tcttaccgag gcaaaataga gcaagacatg ttttcctacg agatggataa tacctatgag 72240

attgaactca tggctttccg tggaagaccc actactctgt tgagctatgt tctggctgct 72300

ctacgtggtg cagaggaatt aaaatacaag catctctatt ccaaaggaat gggtttcctg 72360

tcagcaacaa atatcacaca ggcaaacaca attctggatg gagataagac tgaagttaga 72420

gcgcgtatga ttatgacctt caacactcgt atgagtattg aggatattat gacaactcct 72480

attgaggctg tcaatatgca tattcgatca ttcagagata gctacaatga tcccacacct 72540

ctggatttaa ctctggatag aatttacgta gttcatgagg ttaatggcga atactcacca 72600

gtaactgatg atggatcatt atttaacaac cccaacgaat aaattcattg gaataagttt 72660

ctaacaggag cataaatggc aacttttcgt gaaaaggtag caaccgtaac tttactgtac 72720

ggagctacat caatccgtga aacccagttt gacattcccc tgatccttac tggtcagact 72780

gtcactggta atgcgattga atattacacc tcttctgacg cacttctgga agcgggttac 72840

ggtgttaatg atccagccta tatcatggca gttaaattgt ttaacggatt attcgcacca 72900

agtcaagtga ttgttggtaa gcgtgaagta acaaacttta ccctgacccc agcagttgaa 72960

gacagtgcaa catatgttgt cactatcaaa gaaggcaaga acaagaaaga tttcaaattc 73020

attgctgacg acactgcaac agcacaagaa atcgttgttg gtctgacaga aatgatcaat 73080

gcagacgtag tttatggtgg tctgtttacc gttgctaacg atggcgctgt gatcactgtt 73140

tctccagttg taggcaaata cgcttctctg gatgcaagcg gcttcgctaa agaaaccgtc 73200

tacgctaacg acattaccga agatatcgcc gctctgcacg atattgatag tagctggttc 73260

tgggttctgt ctgactccca cgctgttgaa gatattcttg atgtagctgc ttgggtagaa 73320

gagaatgata aagtatactt cttctccagt tcagacgttg gtatcactac taaagccgaa 73380

gacaacgttc ttgagcgttt gaatgatctc ggttacgtca acacctgttt cgctttgtgg 73440

atgagcgacg cggattctgt attcccagaa gctgccgtag ttggttctat ctgctctgca 73500

cagcctggta ctacgactct gcacggtaaa actctggttg gagttgaaat cgagaagctt 73560

ggtcagactg cggaaaataa cattgttcaa cagaatggta acatctaccg taaagaacac 73620

gcaactctgt tctaccgcga tggtctgatg gttaacggct tctttgctga ctacgtagtt 73680

cacgctctgt ggttcaaagc tcgtaccgaa gaatctctgt tcactctgtt caaacagcag 73740

tcaatgttgg gtggtggtgt ccgtgctact tccaatggtc tggctctgat taaacaagcg 73800

gttctggcta acccgatcca agttggtatt ctgaacggct ctatttctaa cgaagtagtt 73860

atttcagaag agactggtct gcgtgttgac ctgaagccta tcgtttacct tccttctcgt 73920

gctgacatga ctgattcgca gattaatcag cgtctggttg acggcatgaa gattgaatac 73980

gtttacgcag gattcttcca ttacgttaag gttcaggttc acgtcctgac caaccgtact 74040

gcaaactaat aatttggcgg gggaaacctc gccttatccc taaaggagat ccaaattgga 74100

taaaatgctt actggcgtaa tggcctatga tccttcccaa atcgttctgt ccctgggtgg 74160

atgggagccg tggggttttg caactgacac taaaattgta attgcaaaag ccaacgatat 74220

tattaaccca tatgctggta ctgatggcga tgtttctctg gctcttagcc gcaaccgtct 74280

cggcaccatg actatttctc tgcaacgtac atctccggca aacgaagttt tggcaactta 74340

tgctcaaaca atgtattcta ctcgtcaagt agcattcagc gtttacctgg aagatcctcg 74400

tggttactac attagcacca ttggttggat tcaatctcaa cctgaagaca ctatgggcga 74460

aatgattaca gagaaccagt gggttatcgg tctgaaagat gcaagcctgt tacgtaatga 74520

agttggtgta ggtgtgagtg ttcttaactc catctccgca ctgaccattc aataataacg 74580

aaagggcaga aatgcccttt ttatttaact aaaaagagag gaaactatgt ccgaacaaca 74640

agacgatact atcaaacaat atgcacgtcc agaagaaatt gtaaaaatct atttagatga 74700

aacccgtttc gtaaccttcc gtattctgaa gtggagtcct tcaaaggtct tcgaacgtat 74760

tccagaactc ggtagcgttt tagctgtacc tatgaccatg tacaatactg cccttatgtc 74820

ggctgatgaa gatctggaat ctcgtattgc aatggcactt attcaattgt tctctggtct 74880

tgaagagaag aaccttaccg tgttcctccg taagatctta gacgaagtat ataccgaagc 74940

aggacataat gtcgctgata acttcgacga actattcttt gctcacccag aacttgtcct 75000

cgacttgaca gcaaaagttc ttgaggtgaa ctatggccct tttttcaaac gaggtttcgg 75060

aaaactgttg acccagttcc aagcggttca ggatctggca acgaactaag tccagccatt 75120

cgtcacgcga tggaaaccgc agagaagact cttacgttga agtggtatga gttcattctc 75180

tgcaaggttg tgaagaatac ctcggaaaca cgctccaccc tggatcagta cggggtggat 75240

tatctattgg ggctgtacga gtacctcact tatgaggagt atattgaagc tctgtacacc 75300

aaagacactt caataaagaa aaagcaacaa gagaacgcta ctcaaatgca agacatgttt 75360

aagaaaatga tccactaaca tgaggaaaat actttgttag atataaacgc ggctaaaatc 75420

agtaacgtag tcacatttaa ggtggacaag aacagtctca aagaagccaa agattctgta 75480

gagagtgtta aaaagttcgc tgaaagaata cagcccacct taaacatgag taagtttagg 75540

cagcagatga aagagatcga aagagaaatg cgtaaagcgc agactttccc tgctcgccct 75600

gctccccctc ctactccaaa aggtggtgga actacctccg gtcctggtcg tccagctaaa 75660

ccaggaaaga aaactccaga agaacgcgct gccgagctac aggctcgtcg taatgattta 75720

ggcaatctcc gtatggagaa ctttgaaaag cgcggtatgc agtataccaa agcttccata 75780

gatgcgcaac gccaagccaa acaaatcatg gatgcttctg tagaatcgta caaacgtgga 75840

gatatcactc ttcaacgtat gaaccaaaca ctggcacacc agcttgataa tcttcgtcgt 75900

tctcaccgag agaaagtcaa agatattgaa gacgaggtac gtggtcgtcg tagagttcgt 75960

cgtgaattag aagcggaagc caaacaacgt caacgtatgc gtgataaaga gctacgtgat 76020

attgagcgta accataaacg tgaactggca cagcaacgcc gagataaaga tcgtcaatac 76080

tcgcaaatga gagatggcgc tttgggcctt aatcctcgca tgatggtttc atccatgatt 76140

ggtgcaggat tgtttgcagg tggttctgtt gtaggtaata cgttacaaac tgctaacgat 76200

cgtatcaagt tcataggtcg tggtgcagag aacgttcagg caaacgccaa cgccattatg 76260

acaatgactg cttggggtga acagaacggt gttgactcgg caaacattat taagtcaatc 76320

gataacctta aagacgttcg tgaacgcttg ggtaactcaa tgatggcttc tgaatgggac 76380

gaaaagaaag gaaaatggcg tggcggtgat aacggtatca acgatatcat gaacatgttt 76440

ggttggaacc cttcacaaat taaacacatg cagaacgatc cgttagcttt catccaggca 76500

actgtgaacg aaggtgagcg tcgtggcatg aactcggcac agattggtcg tttattagaa 76560

aatctgggcg atgatctcat gcattaccaa cgtatgttta gcaagaacgg agaaggcttc 76620

caagaaaccc tccgtatgct tcaacgtact ggcgctgcat tgaccaatga gcaaatggaa 76680

gcttctaaag agtacacacg cttgactgcc acggttggct tggtcgggca aggtatgcag 76740

aactatttcc tggaaggatt tatgaacgca atacctaaag acgagtcctg gttggacaac 76800

gtgaaaatgt tggataaagt agcgaaagat ttgggtgaag agatgggtaa gttaactgtc 76860

aatacagcta aaactgcaac tatgttcctc aacatcttta caggactgga tagcttcatg 76920

cgcgagaact tcggtttcat ttatgcagct acaggaaaag atgccagtgt gtgggatatg 76980

gcgaacagaa cctttaaccc ttttgcggcg gtttcagatg gacttacctc taagaatgaa 77040

gaaggtgttt ctatttggga taaggttgtg ggatattttg agaaagatgg atcaggatct 77100

gcggcaaaca attacacgtt taccggagaa gctattcgtc aagatgcaca aaatttccag 77160

tctaaagcta tgcagtacca gacaaatccg accttcaaca ttacgttaac accggaagtt 77220

gccttgacat tacaaagcga tgtttctcgt ctttccgatt acattgactt caatgctaaa 77280

gcttcttctg aatcgttcat gcagagcctg actttgcaga cgttaagtgg acagtcaaac 77340

agcaactaaa gaaaggggct tcggcccctt ttttcatttc tataatcccc tccccctctc 77400

atcccatatt tttacattga cataacggcg acatttgtgg tatcataatc aatatgggaa 77460

gatcttgcat cttcctcttt gcattaccat tactatgagg ttatcatggc agcaatgact 77520

ccacaacagt cggtaagagc cgtctacaaa tccgagcaag aggtgaactc aacacaaaat 77580

acgaataact ccgcctccac ggtgaaaggt gataacggtt tttgtatctt agccagtgtt 77640

tacaatagct caagtgatca atacatacaa aattttatgg cgattacttt cgactccgca 77700

ccggaagttg gagtaactcg ttcagccgat atcacaagct tccctgttga atcaggtagt 77760

acagtgagtg ataacgtcaa aataaacaac aacactttta aattgacagg tgttattaca 77820

gaaacgcctg tacgtttgat gcgtgatcag ctttacagtg caggggttaa tggaacacgt 77880

atctcccaag caatcgagta tttggatcag atttttgatg ctcgtcaacc gatagttcta 77940

ttgacagaac accgtacttt cgagaacgtg atcctgaaag gttacactta tgaatatcgt 78000

tcagaatttg ctatgcagtt taacctggaa ttggaacaga ttcgtttggt cagtactgca 78060

acaactaacg cgatagctgt gaagaccgct ccgaacaaat ctactggcgg tgaggttaag 78120

agcaaggctc caactggtgg tgctaaaatc actaacccaa ttaaccaaaa tgctgcaaga 78180

gatactaata caccgtcgaa taacggaggg taatcgtggc aacttattct aaagcaacac 78240

gaagcacaac aaactctgga tataaggctg tcttcttgga agaccgttct aattactttg 78300

cctggtattt gaagacagaa gaatatccag accaaaccta cactgtaaca ttggatggtg 78360

tagattatga tatacgttta cgttggaaca ctcgtgatga agcctggcag tgccttatag 78420

gtttatcagg agacgaaccg agcgtaacgt ttaagataac caacggactt gacctgttga 78480

agccgtacaa gtatctcgaa ggtgttcccg atggacaact ctacatgatc gatacggtaa 78540

agattaacgg tcgtcctggt tacacaacta caggaattga aaaacgtttt gttttggttt 78600

acatagacaa tctatcgaac caagcgtaat agttaagggg agattgtatg gcagaaagag 78660

aatatacaaa agcccctgtt cccaatctga acagggcgta taaactgttg attggtacgg 78720

caacgaatac cgtgcagaaa aggacaagca actcatcctt taaatcaaca gtagatttgg 78780

atactaaatt gggaagcact acaaaatctt cttctaattt gtatctactt gaacatcatc 78840

aaattacttt caatatcaag aaagataata acaaagatcc taaccaagcg gagattactg 78900

tttacaacct gtcagatgac acagtaaact acatcaaccg aggcattcgt aacaacctgg 78960

cagtagcttt agcagttgga tacgaagggc aagaattggt tatgattttc aagggtacaa 79020

ttcagtgggt aagtgatacg tttgatagcg tcgatcgtaa aactgttctc cattgtctgg 79080

atggaggtat taacatctct gaagcccgta ccagtcgtag ctaccctaaa gggacgaaag 79140

taaaacgtgt tgttgcagat cttgttaagg atcttgggac aacagaaggg aacattcata 79200

tcgacaacga tcagaccctt tcttctgcga cagcgatgtg tggaaatact tcacattacc 79260

tggaacacat ttgtaagagt attgatcata acgtgtccat tcaagatgga tctgtttatg 79320

ttacgcctcg ttctcaaatg tccaatgcaa gaagtgccta catcagtcct gaaacagggc 79380

tgataggaag cccagaacca ttccacaacg atatcaaacc tgccaagaag gtgactaaag 79440

cttctaagaa agccaagaaa cctacagacg gtgtgaagtt caaatgtcag atgaatggtg 79500

caatcttacc tgaaaaaact atctggctca agagtcgtga ttacgatggg ccatttaagg 79560

tggtgtcagt atcgcaccat ggagacaagg aaggtagtga atgggttaca gaagttgaat 79620

gtgtatctgt gtctgcgatc atgacgaagg aaggtaaata atggatgctt tgaactttgt 79680

cacagcgatg cgtggcatga tgacagaaca actggcagag gtgcacacct cattaccagt 79740

acgagttaca ggtgttaact acggtgcaaa aacactgaca ctggaatcca ttgtgaagaa 79800

tacgcgatca gaagaagatt ctatcgacta tccaaccttc cacgatgttc cttttatggt 79860

taacggagga ggtacagggc gtatctcctt ccctattaaa tccggcgata ttggtgttgt 79920

tattttctct gaacgagatc cttcgaatgc aatgcaaact tctggagaag cagccacaac 79980

gactacatta atgcagcctt gcggtttata tccgatttgc tttattccta agatttccac 80040

aggcaccgat tcaacagaag cagtagattc cgagaaggtg gttatttcaa ataacaaaaa 80100

cacatatgca tctttcgatc cttctggcac catatcgata tggaatagcc aaggaatgaa 80160

gattgatatt actccttctg gaataacaat gacagacggt tccggcaccc tggatttgac 80220

aggtggtgtc atgactttca aaggcacgtc tgcaaacatt aacggactac agattgatca 80280

gaacggattg atgacagacg gtaatggtat tggcttccat acccacaccc atacggttcg 80340

caacattcag agcggaagct caagtgcgga atccttgaaa ccttcaggag cataataatg 80400

ggtatcccat atgatttaaa attgggatca gacaacgacc tcatcatgga tgatggggat 80460

cttgttttga caaccactcg taccgagatt gcagcgcaga cattagggat caccctcaag 80520

acatatcgtg gagaatggtt ccttaacacc acgttcggtg ttccttacct gcaagagatt 80580

atcggtgtag cacgaaagaa agatattgtg gaccgaatct tcctggcaga gattgccaag 80640

aatatttata tcgacacaat caattcttac aaatcttatt acgatcgtga ccaacgctat 80700

tataatatgg atgttgttgt cacagtaggc gcagacacag taacaacaag tttcaacacc 80760

caaccttctg aagaattcat ttatccagaa gctggcgatg acaatgcggc aatcacttgt 80820

gaagcataca ccattgttga atcttctaac cgtctgtacc gcttcattaa ctttgttggt 80880

ctgcctgaaa atacttattc tacttggtgg aatgagtggg cgactgaaac cgctgtcgaa 80940

cgtatgatgg ttagcgattc aggttcagtt ctttcaacaa ataataactt cggccttacg 81000

gctatgacag gagtataaga tggcagattt aacaactatc aaagttacag acttggcgca 81060

aaccacgtct gtttcagatg cagatttctt tgtaacggat caggcgggga taaccaagaa 81120

aacgtccctg cgtgttcttt tgaatggtgc tggggttact cgtaaccgtt taagtacccg 81180

tggcaccgtc accagtacct tagctttaga cttgggatca gcagagtatt tttatgctac 81240

cctctcttct gcttcttgta cgatcagctt tacaaacatt cccgttacca cagcaacagt 81300

tttaaacttt agtctggctt tgcgtcaagg tagcggggca aataaagtgg tatggccagc 81360

ctcggttaaa tggagttttg ggcgcacacc tgttttaagt tatcgtcaag gtgcgatcga 81420

tacttttgaa tttatcagtt tagacaacgg tgcttcttgg attggaagta tggtaatggc 81480

gggagtgatc taatgtctca aattaagcac aatattgata acactttcca gctaatcgac 81540

ggattggtac agttcttaga aagaaatact gggttgacaa ttgatcctac ggttcaacat 81600

tacgtgatca acccacaaaa cattctggct aacaaccgtc actttatcaa ctggacagca 81660

caggaaggtc agccaaacgg cgatgctaca acagaaggtc aatctgtgct tatcacaggt 81720

tttgcctatg catacttagc caccggagat agcaaatatc tggactccgc taagaaatat 81780

tggcaagcct acattgacgg attcttcggg gggcaaccta tccccgatcc tccgcaaaag 81840

tatcgtccta actggatcat caacgggaag gaaccccgtc tggcccacta cccattaacg 81900

gatgatgggt atccaactca cgggggcttc aaaggaagtc tgatgacttg gacaaacggg 81960

cgtaccgtga ttcctaaagg tgctccacac tggggagaat acttggataa agcttggttt 82020

gctttcaacg gtaacttagg atggaactct gttaatgcca cagtttatgc gaccaacagt 82080

gacggttcta ccaactggcg tgagtacgga gatcaatggg atgttgattg gattattgat 82140

cggttgggac gtaaagtgga ctgggatgga aacattctat cacaagggca cactcccgct 82200

gaatacggta ctgtccaact gaagaacacc acagtcaccg gatcatataa attcaactat 82260

gcaacatgta accctgtaga gcatggtggt tatctcatgg gacgtaacga gatgtggcat 82320

aaccgcccag tcaacgtacc tgtagagatg ggcttccaag ataacgcctc cgatgctgaa 82380

acttggtggt gtgatgcgaa ctatctgatg taccagatca ctggcgaaag gaagtattgg 82440

ttgtgttggc agtcttcatt actgctttgt caagactact ccaacattga tatgttcgat 82500

aagtttttcc gtaagtcaac tactgcgacc atcccgttca cagatggcat ttcttatgat 82560

tacagctacc catctacagc aatcccaact tactccaggg attcgttagg gtacacggtt 82620

attcgtcaga acgtggcagc acaaactaca ctggagcaac aggctgtctg gttccgtgtt 82680

ggggatagct ctaaactgcg tgttcagttc caaggtgttg atgatgcagg ggagggtatt 82740

gttttccgtc cagagttgga gttgagcaag acgaaaagtg agactgacac agttacatat 82800

cgttgtggac tgcctttagg tgcagccaac atgacgacaa tggatattcc tctatccagc 82860

ttcatgcgta ctgaaccagc taatggtggt gtatatatta ttgcagaccc acgaatgatc 82920

attgactggg gtgccaatac tgtggtacaa tacaaatatg caacagatat agctggaacc 82980

aatagcgacc agattgtttc tgcaaacatg gacgccgatg gcgggataac aatcggtttc 83040

tggttgacac caactaaaac agcaaactta aaatctatca cttaccgttc ctatactgat 83100

gatttcaata tcacaataac ggacgcgaat gggtggagat ggtgggcaat gctggagaaa 83160

actggtggac tttggtcaac taaaacgtta accgcagcaa gtttccagtt atcagcttgg 83220

caaccagatc attcagatac agatcccaag cctacggtta tagacctttc tgctgtagag 83280

cagattaact tggttctgga tacagaccct gcaaacggtt tgacaggcgg cattgacttc 83340

tattgtgtta acgatatacc ttctcgttac tcttctgctt ccggtgaaga ttacacgatg 83400

tatttcagga tcacagttca gtctgaacac ccgcacactg ctaaacttgg tgattgtacg 83460

atcattgact acaaactgaa tagcctgaac tacacaccag gaattattcc gttctcgaat 83520

attagtgacc ctaatacatc tttgtatgat gggtggcgtg ggattccata tcctggctac 83580

cagtatccgt ctctgtattg tttccaaggg cgtgatattg attggacaag actgaacaac 83640

agtattgact tcttgtacga ttcacaaatg tggttctaca ataagttctc tcctgtaatg 83700

cctggcccta tggcgcaagc ttatgtttgg gatcgttggg atgctttgaa gtatggtact 83760

aagaatgagt tcaccatgta tcactggaat gataaagctt gggatggata cgaggctcgt 83820

gcgttctatt gtgcttgtca tacagtttac gaactgaagc agcgtgggga agatattcca 83880

accaagcttt tgactgtttg taagaactgg atcaactatc tgaaatggtt ccaagataac 83940

aacgaaggaa gaacaccgac aatcttctac cctactggag aggtaactgc tcctatagac 84000

gactttacca gccacatgtc tgcattgttt atggcgggtt gttctatcat gggtatggca 84060

ggatatgaca cagagatccc taatcttaaa atcgtagcag atcgttgctt cgaactgatt 84120

caggaaaact acattgtctt aggtccgaac cacatcatga atggatgttg gagttctgca 84180

ccaagagcaa gcacagataa cggggtattc tttggcttcc acgctgggga acttttacgt 84240

ggttttggtt tatatgcttt atacaaaaaa ctgaatccgc aaaatgccct tccactctta 84300

gactcaagca atattccggc gcttaccgtc ttgacaataa aagacgtaac cgtggatgcc 84360

actgaataag gagcaaaatg aaacaggcaa catttactgt cacaagagac aaacgagtaa 84420

atgtgagttc gttcaactac aagactgtcg atggaacggc agtctccggc agagattatg 84480

tagcgaagtc gggaacctta gtgttccctg ctaacgccga ctctgcaaca attgtggttg 84540

atgtttacga acgaccaaaa aactcaatag atattaactt ctttttagaa gtttctggta 84600

tcagcgaaaa caacacgatg gttgaaacca agatcgagtg tgtcattacg acgagacaag 84660

agaacaccac tccgattaca tggacctcaa ccaaagctaa aatgttctct cctaagttct 84720

ggactatcga ctcccaagca acagagagtt gttccataat ttccagtggg gattccttta 84780

cggcgtactt tgttaaccgt acagcagcag gtttggcagg tgttctttgg cacacaacag 84840

acacctttga tcataagggc gtaggttttc ttcctcacca agatttaagt gaagagaagc 84900

tctggtttaa attagaactt agtgcaaaca tgccagggtt caatgatgct ccgttggtcc 84960

caactattac tttaacattg gttgataaga cgattcacta cattgatttg agctattatg 85020

ctttcaatgt atcagaagat ggcaaaacag cagatatccg cttagacttt agcaatatta 85080

agtctggacc gaacaatgat attgaagtgg acaccacaca ggtggacaat ttcttcttct 85140

ctttgatcac gacacgctac caagacataa cagaaattgt tcagatgaac aatatgcagt 85200

cgtcaatgaa attcactttg cttgaacctg acactgggtt tagtcagatg gacgttagca 85260

atttggtaca agcagcacac gatattcgta tgtgcacatc ttacgatgac atgtataatc 85320

ttacccctga acgtgttatt tccaacactt atgctttagg gtatcgtggt atgataaacc 85380

attacaacgg aatgtcgcac tactaccagt ttgaatggga cggcaatgat ggctggcaat 85440

taaacacttc cgtgtttctt aacccagcga ctgaagcgtg gttgaacagc ttccatgcaa 85500

tcgccaaagc ctccggctac acagttatga actctttgag ttttgaattg atgagcacag 85560

tctgccctgt tgagtgggtg cagcacgatt ggaacgatgc tttagctgct acaggttatg 85620

tgcctccgag ctatgtgttg tctcctacaa ttgatgaggg tatgcaatat cttcagaatg 85680

taattaacaa gatcgcctcc atttccacaa ctaacgacat tccggttgtt attcaaattg 85740

gggaaccgtg gtggtggtac aatacagcta ccaacctgcc ttgcatttat gattatacga 85800

caaaactggc tttcaataca gaaacaggtt tgtttgcaca agatgctggg acgattgata 85860

actataaaac aggaagccca tacgatcagt attacgagtt cttgtctcgt aaattgggtt 85920

tgagagttac caagtttgca acggacacac gcgctgcata cccaggcgct caagtgacat 85980

tacttccgtt cttaccttcg atcattggca acgggttaat ggagaaagta aacttcccta 86040

aagattacta cattccagaa gctttcgatt tctattgttc tgaatgttat gattggttgc 86100

ttcaaggtaa gatgattaaa gcctatgatg ccgtaacaat accgcacgag actttgggat 86160

tcccttacga agatattcac tacttggctg ggttcgtacc agatgccaca ttagcaccgt 86220

tgtacggctt tgatcctgat tcaaattacc gcacatattt gtggaaaatg atcatcggga 86280

acatagtcct gaacaatagc aagttcgaag gtcttaagca atatatatgg gcttacccac 86340

aaataatgca agactctatt actgtggtca actctcattc gcaagttttc tatatgggca 86400

atgttccttt aaacgtttac ctacaggata cagtttatgc ctaaagagaa tgggtttaag 86460

aagtttgttc gcggaatgtt ttgtacttac cctggaaacg gggtaagttc cactaaagta 86520

tggaacgccg taggtatgat gacaatgaca ggtatgttca tctttttagg aatagaaaaa 86580

gaaattcctg aatggatggg ctgggctttt gtttttatgg tcactccctc acgactaatg 86640

aaaaatctaa tagacctgcg ctggggtagg tcttccaccc ctgaacacaa caaagaggat 86700

gagtaatgga tgaatatggc ttaaccgatg cgggatttgt aatcccgaca ttcgatgatg 86760

tacttaacaa ctatatgact gcacttaaga acacttttgg tgccgatgca gcgacttcgg 86820

aggatactgt cttcggacag ttgttccgta ttattgctta taccgactac acattatggg 86880

aaggaatgca aggagtttat aatacccaga cactggatgg tgctgaaggt atttaccttg 86940

atgaaatctt ctccaagcgt ggtttctatc gttctgccgc tgcacctggt tcaggttttg 87000

cttatgtgaa aagcaacaac aaagcccctt ggacttttga acttggcacg gatacgtatt 87060

ttaatgcaga caacgacatg aactattatg tcagcgcacc taccatgttg aatgctaaga 87120

tagcagcata tcaaatctcg cgtgcgaatg caacagctac aggagtggct aacataactt 87180

tctacgcaca aagtgcaaat gatggtggtt tgaatagtcg tgtactctcg ccagcctctt 87240

cgacattcat tacagatctt gcaaacttta ttcgagacaa cctctccact acggatcgtg 87300

atttaatcca tacagacgca agtaacgttt acgttggctt cgcagcagcg ggagatgtag 87360

aacctgttgg tttggtaaca ccaataaaat tctacgcttc tgttcctgtt gggacgaagt 87420

ggagtgaagt tccggtggta ggatcagaag cagggtacaa tccggtaggt gtggaatcca 87480

tttctggaat ctcttcaacc ttcacaggct accttgattc tggtaacttt tatgctttct 87540

cttcaggacg agatgtagag acagatgcag agttccgctc acgtttcaac gataaccttg 87600

atgaagcaaa cgcaggtaca cgcgcggcta tcattaaagc tttgttgcag actgacggcg 87660

taaccaaggt taagatttac gacaacccaa caatccacga tcaggaacat gcacctgcaa 87720

tgagtttcca gacaatcgtg tacggtggga gtgccgaaga agttgctaaa gtaatttacg 87780

acactaaacc aatcaatacc ttgacttatg gcacaactaa cattactgtc atgacggaag 87840

atggtggaca agagattatc cgcttctctc ctggtgctac tgcaacgtac tccttgaaac 87900

ttgtatacca gacaatcaac ggtacaacgt taagcggaac agagaaagaa aacattgttg 87960

aagcactgga taacctgcaa gcctacttcg aattgggttc ttctgtaact aacgaccaga 88020

ttaaaggcgt tatttatgca gcacttgctt ttgggcgctt gacttctctc cgtgtttatg 88080

ttaaacttaa taatgaagac gacagtaaat atacagaagg aaacattgtt cctgcctaca 88140

atattgtccc gtctttcgac cttgataaca tttcctacga gtatggagtc taaaaatggc 88200

agtgagtgtt gaccatgtaa cgacttggga gaatatcaat gaagacgtta aagatctgtt 88260

gattgaagat tttaaatctt cccctaacat tgtcaagctc ctctacatcg tatcaagtga 88320

aaagcaaaag atagatcagg caatgatcta tcttgctaaa caccgtttaa tatctacagc 88380

cacaggggat tatcttgatc tccttggtga agagatgggt attgaacgaa atggttctga 88440

tgatgaagag taccgcacaa ttttaaaaat cagggcttat cgtgttgcct cttcggggac 88500

tcgtggggac attattgata tcttcgcccg tttcacaggt acaaaacgag atacaattaa 88560

cacatacgtt gggttgaata aatcctttga tgtgttcttt tataacttgt gtcttcagtc 88620

tgatcaagct cttgagcaac ttttaagtgt gttcccaatt ataagcaact accgactggg 88680

agctaaagcc ggatcagctt ttggattcac ctcttattac gctaccaaag atcctcacgg 88740

tattggtggt ttgggttcct tgtacttgga atctacagga gatgaaactg gacgcatgtg 88800

tatgcttctg aaacaaatcg attaaggagt tccaatggcg aagccaattt actatcctga 88860

ttgggctgta gaaacagtta ccctcccagg aacacaaaac atgaacaaga ttcgacctaa 88920

agaaatcatt cgtaccgtgg gtatggatta tggtcaaatc atgacatgtg aagaaatgaa 88980

ctggatactc aacaataacg gattatggat tcgttacttg gttgatgaat tccttccaac 89040

attaccaaat acatatcttc ctctggtcgg tactaagata acaatggcag gggatgcaac 89100

aggcgaagta acctggaacg ggaatgcagc agtaacgctt tctattcaag ttgtagacaa 89160

cagtcacaac cacttatcct ctaacatcac agatgcaaca agcaccacag taacccctaa 89220

tgtattggtt aaacgtgacg gcggcggcgc tatttatgct ggagatgttt acgtaaacgg 89280

tacagctact tcagatcctg ctacaatatt ctttaaaaac tttgcagggc aaactaacgg 89340

agagatttct tccgagtaca ataatggcgg tcatctttat atataccgca ggaaccctac 89400

caatggtaac gtatcctgtg cagtacgttt gtatgatggc ggttatgttc tgattgataa 89460

tccacaatct aacagtggac aagatactgg gaacggtaat gctctggttc gtttagatta 89520

cttgaactca cgtttaagct ctttacagaa caccttgcaa acgaatatta actctgttca 89580

aaacaacctg aacaacgtta acaataactt gaactcacgt attgacagcg tgaacagtaa 89640

cttgcaaaac cagatcagta acaactataa ctatgcgaat ggtacttttg ttcgtgacat 89700

acgttatacc agccaaggcg tatattaccc accaggtaac gaggtgtctt ggacatatat 89760

ttgttcttcc ggctatgttc tttctggtat tattgttcag gaaacaggtc gtagttctgc 89820

ggataacatt ggtggtgttt acttcaaacg tctccagcga aacgtgaacg gaaactggta 89880

cgatattgga ggctaaatgc tctacactaa ttttaagaag tatatcccag aagatcccgc 89940

cctaagagaa ctcgtagaag tagagaacat tttgttccta ctttgcgacc agggtattga 90000

ttggtatgaa ttgcgcgatg gtttgctaca gaacgataaa atgaaagtcg tatttgatga 90060

gcaaaatcgt atagtttttt ggaacacaga ccctactctg ttgtgtcccg aaaacatgtc 90120

tcttgcacaa gttgatttcg agattacaag cttcgaagat atccgtgaca aggttttaga 90180

cttagaaact cttacactga aagataaaga gtacagtcgt gaacaaatga ttgtcaaggt 90240

ttctgctgaa ataaagaata agcaagaaac agccgttgcc cttatcactc ctttgcaata 90300

tgcaaaagac cttgatatag caacacccga agaacttgcg gaactgaagc aactccagat 90360

ttatgttgtg gaattgaacc gtgtgcctct tcaagaggga tatccttacg aggttaactg 90420

gcctacccta ccaacaaaat aagggatgca aatatggaat ttcaagaagt ctggggtctt 90480

gctaaggacg caatcatccc tctaatcggt ttaatctggt tcctgtttaa acaaaaaata 90540

gataagtttg gtgttgattt ggagaaggtc gaagacaagg cccgtgagtt ggaaaagaaa 90600

cttgttttcg tcgaatccac ttacgcaacg aaagctgaac ttactcaaat gctgaatcag 90660

attaactcta cattactggc gaataatact actcttgagc agaagattga aaaaatcatg 90720

gacctcaaga atgcaacgat ccagatgatg ttagaggata ttactaaacg tacacagagt 90780

aagggttaat tataaatgac actattccca acatatcccg ctacgtcctt ggaacagggc 90840

gtagatctgg taatcttctc ttctaaccag atacacgata tcatcaacgg aagcgcaaca 90900

gaaagtattg atacggaatc aggattgatt ccgactttac gtaaggcttt ggttgataac 90960

ttttacttca aaagtcctat tgcctgggcg caagggacta atgaaacagt attcaaccaa 91020

ctccgtttct atcagaacgg aatcttgagt ggatactact acgctccagc agcaacactg 91080

gtgaacccta tcccaatgca agggactcca gtcggggaca ataactgggt gctgtgggga 91140

atgcagactg aacaactgcc aagtgaagtt attccttggc tgtatacctc tgcaacaggt 91200

tatgaaaaag taatcagtcc tccttatgtg ttcgatagtg ctattgtgac tatcaacggc 91260

gttatccaga tcgtgggtga tgcattccgt attgagaaca gtcagatcat cttgtccgaa 91320

ccacttggac atgatcctgc aactggatta cctaatcagt tgtttgctta catcggtaag 91380

attgtagcaa gcgcagaaaa cgaccctaac ctcgaaaacc gaatttctaa cctggaaaca 91440

actactgaaa cattgaccga agatatgaac gctgttcctc ttcagacaat cgctcgtaaa 91500

tatggtttcc tgaactatga agtggcttac gcaaaatcag gacaagcttt aataggtctg 91560

aaagccctgt atgatgccac taaccaaata gcttacaccc tgccacctgg tttaactggg 91620

acatttgtta gcttatcaga tactggtgtt cttacccact cttctggtac tgttgacttg 91680

gcagcattag cagttgaacg tcagcaatat gtacatctgc ctaccagctt cggtaatgaa 91740

gttaccctga cactgaaaca ccagactatc ggcatgggtg caggtcgtta tcgttgggct 91800

ggccctttgc caaaaactct ctctgcgagt gacacccttg cctcctctgg cggcgtaggt 91860

ccgaatgctt gggtactgac aaacacagaa ggtctgttaa ctccacacgg tggtaagttt 91920

gatgatctgt ttgatgttgt ttacctgtcc gagtttgcaa acagtgcagt gtacgctact 91980

ccagaagcag ctttccaagc ggctctcgtg gcaacagcag caaccaagag caagatcctg 92040

aatgcttatg gttgtgatat gaccttcact acaagttctt ttgatattac tgggatcacc 92100

ctccttggtg gtgtcttccg tggacaacgt gactaccgtc tacaaaactg taaagtggaa 92160

ggtacaactt tccgcaactc ccgtgtaatg tactggggtg gcgatgtgcg tatctatgac 92220

tgcttgtggg atggtgcacc tcgtccagga caggttggta gcttggtatt ccagggcaac 92280

ccaattccag gtacttttga gattgacaac tgtacgttta aaaacggact ttacggtatc 92340

cttcaacaag gtactggcga accagttagt cgcggtgtct tccgtaactt gacgtttatg 92400

gatatgcagg gcgatgcaat cgaactgaac gtaatcaaca agcattacga tgaaggttgt 92460

gttattgaga acatctactt gtctcacatc gacggaacta acgcaccaat tcctctttct 92520

aactggggta tcggtatcgg tgtagcaggt aaaggtccat acggctatga cattccagat 92580

acgcagtatt gtaagaacat tacaatccgt aacgtatttg cgaaacgttg ccgccagatt 92640

gttcacgtag aagttggtcg taacatttct atccagaaca tccacggtga tcctgaccaa 92700

acagtttctg ttggtacagg tctggcaact ggtgctgttg taatgtacgg ctgtaaagac 92760

ttcacgatcg atggtgttta tggagatcct gttacggacg acaacacttt accaagtgca 92820

atccgtatga tctacctgga gtggggtact aacgcagttt taggcgaaga cggaaaacct 92880

gtggttcctg cacaaggtcg tccatcaaac ccttgcttca actactccgt gcgtaacatt 92940

catactccta atggacgtgt gttcgctggc gtatcagcgg gtccaggttt tgagaaccgt 93000

gttagcttcg ataacatccg ttgtaacgct ctgcaattat ttggtatcgc ttcttggttg 93060

tcaatggcaa acatcacttg taactctttc gagtgtgtgg gtcagcctga atctggtcca 93120

ggtactttct acgatggttt cttccgtaga gagaagtccg tactggagat ggtgaacgta 93180

aactgctatc caaatggaaa cacaatggta actggtcgtc ctcaatggag ccgttgccgt 93240

tactcggata tacatcgtat caactgtaac gttgaagcga atatgtatac gaacatcgca 93300

ggtggtattg gtgctctggt aggctctact ggtaaatctt actacatcga agcaatccca 93360

ggtcgtaaca ttgacgggat gcacttccca acagggaaag agttcgataa aggagatatt 93420

atcgttaaag cagataacac gttcttcctt gtaactacaa gtggtgcgta catcccagac 93480

attccagcct tcggtatccg agcgacaaat gttggtgata aatcgattgt gcagaacctg 93540

acacctaacg gtacagcgac aaatgcttct tggttatatc attacccact gtcagcaggt 93600

acacgtatcc gtatcccagg tgcgggtcca ggcggatctg atttggacac agtaatcact 93660

cgtgcccctt atcaggataa cgatacttgg accaacccag tgaaggtgga tattgcagat 93720

cctatccaaa cagcgacagc agcaggagta cgtatcacaa ctattcctaa tagcgcaagc 93780

aacgttgcgg ttggtaacac tgcggtaatt cgtcctactc cggtgattga tactccgacg 93840

acataagtga aagggggctt gtcccctttt taactcccaa cgataaatga atgattaatc 93900

aggagtaaat aaatgctttt aacaagaccg aatagctcga ttgcagatat ggtcgaagct 93960

acgtaccgta aacgtcgaag ctttgcagcg ggatttactt tacgtaatgc ccgtgaagtt 94020

ctgcttttag atgatgacgg caactattat aaatggaaag gtgcttttcc gaaagttgtt 94080

cctgagaact ctactccggc aagcacaggc ggcattaact ccactggatg ggagagcgta 94140

ggcactgctg atgatatcgc agtaattaat gcccgttttg caataatgca gcaagagatc 94200

aataacatct cttctcaaat ccctgaagct gggaaatcag cctaccagat tgctctggat 94260

aatggtttcg taggcacaga acaacagtgg ttagcgtctc ttaaaggtga cactggcgca 94320

caattaaata ttaaaggctc tttgaacaac atcaaccagt tacctacaac aggtaatgct 94380

ttaggagatg gttggattgt cggagatgca atttacgctt gggacggcgc aacttggaaa 94440

atgatttctt ctctgggtcc agaagggaag tcagcgtatc aagtgtggtt gtcagtaggt 94500

aatactggta caatttacga ctatctggca tctttgaaag gagatcgagg ggatgttggt 94560

cctcaaggcc cacaaggggc agcaggtctt aatgcgaatg gttttgatta tcgtggggat 94620

cttgactcta ctgccaacct accagcagcc aacccaaata acgtaagcca agcttactct 94680

atcggaggct acctttatgt ttccaatggc gaagactggg ttaatatggg taacattgtt 94740

ggcccacaag gtccacaggg tgaagcaggt actgacggtc tggatggaac agatggcgat 94800

gaagggaagt ctgcttacca atcttggtta gatatcggta atacagggac ggaagtagat 94860

tttatcgcag cgatcaaagg cgagaaaggc gatcaaggga ttcaaggtga acaaggaaat 94920

caaggtgaag taggtccaca aggcccagta ggtgcaacag gctctggctt ggaaatctta 94980

gacgttctgt ctgatgaatc tcaactccca gaaacaggtg aagttggtga tgcttacatt 95040

atcgacaaca acctttatgt ttgggtagaa ggtgctacag actggatgaa cgtgggtagt 95100

cttggtggaa catccattac cgctaaaggt cacgtaccaa acggttctgc actaccaacc 95160

tacaacaact atggtgacgc ttatctgaca gaagatacca gtaaccttta tatttacact 95220

tacgacaaag atacgaacac cgcctctttt gttgatatgg gtagtataaa aggtgataaa 95280

ggtgacaaag gagataaggg tgatccaggt ccagcaggta ctaacggaac agatggaact 95340

aacggtacac cagggattga tggaacagat ggtaaggacg gggcgcaatg gtttgtagct 95400

gcaaccaacc caaccaatgc tgaaggtgtt attgacgact acgctttcaa cactgcaaca 95460

ggggctgtct ttaagaaaac cgctgctact gtttggacgc aagttggtac acttccaagc 95520

attcctgaag ctcctgaaga tggtttaatc tatggacgtt ctgatggaga ttggattgaa 95580

atcagtggca gcgatcctac ccaagagatt gcaccaacga aagtcagcgc aggttacatc 95640

gagttggaaa caactaatgt agcaagttca ggaacggctt ggactccttc tggggccacc 95700

aacatgtacg tggtgaacgt tacagacgca ttgacgatag gagcatggcc tggagtaaca 95760

ggaaccacaa aaccgaaagc tttctctgca atgatttacc ttttgcaaga cacaacggga 95820

cacaccgtga cactggatgc gtcttatgga gcacttaacg atacggaagt aggcacagca 95880

gcatctgctg taacaatcct tcagttaacg tattgtggtg tgggcgatat cgtggacatg 95940

gttgttgtaa cacgataaca gaagccccgc aaggggcttt tcttttatgg aggataacaa 96000

tgtttccaat cccagtaatg atgattatga cagatgctgg agtaatacca cttccagcag 96060

ggattgtcaa gaaagtaata actttagatt acccagataa cgcgggaaat tctaacagca 96120

gtctggccct tttgctcaac gatgggcgac tgttcacgca aggtgccaac ctttttgggg 96180

agatcgcaga tggaacgcgt agctctcgtt tcaatagctt ctacctggct tcaacggtag 96240

cttcggatgt gtttacagca gatcgttgct tcgttattaa actacaaagc ggtggatggc 96300

aatacgcagg actgacagca ggtttggtag gtgcaaatgc cgcaggagga tcagatgttt 96360

gtgcgacttc ttggacaagt ttcccatctt caattacagg aacaatcacc ttagccaact 96420

tgaaagaagt caagggaggt tacaataaca cgttgtggtt gatgacaaca ggagttcttt 96480

acggatcagg acaaaatact gttgggagtt taggttcaag taacacaaac caaatatcta 96540

ctccacgtca gataacaagc gttgctttgg cgtttgatgc aggatacaac caatgtactt 96600

acctcaataa tgtaggagca cttcgtgtgt gcggggcgag tagaggggtt gcagggaaca 96660

acagtacaac cacagccttc gttacagcta caatctcgac tactgaaaca gtgtttgtta 96720

aggattacat caacacagta tctcaaacga ttgttattgg agatacagca gcaggagcag 96780

gatcaacaaa ctacctgtat gttcgttcca atacggcaac aacgtataca aagcttgaga 96840

acttctcaac aggttttaca tcatgggtaa tacctccgag tagacattcc ttctttgtgg 96900

ggataaataa cactttgtgg gccattggta agaactacac agcgaatctt ggcttaggat 96960

acgcttctgc gagcacagac ccactacaag tcggtaaacc aatcaaacct gaaggtgtgg 97020

cctcttggaa ccttaacaag ctcacaagcg tacacacgct caatatggac gttactaatc 97080

agttgggcta ccaaggtact ttcattgtgt atgatggcaa cctcttctac tccggtgctt 97140

gggtagacgg aaaagcttct tacctgttca acagcggtct taactctaca tctttctcac 97200

ctataactac agatactgta acagggacta ccctggcaac aggattgtct acagattctt 97260

taggtatttg tattgttggt gggaccaaac agttaaccta ttcagtaagt ccttcaggag 97320

gtaagctctt caacgaagct tacacaacat ctgccccagc ttatatgacg atatcttcct 97380

ctggtttgat gacgtttgtt gctgaaggtg gttttgatgc agggatgaca gcattgaacg 97440

cagcaggaac aaccttatcc gacagttctg ggggatatgc ttctgtactg ggggtgtata 97500

ctccgagcct ttcagctatg gatgaaggaa caacgcagac attggtggca gaaataactc 97560

cagcaggggc agagaacctc cctggcatgg ttgttgagta tttctctaat gaccctacgg 97620

tagcaacagt tgaccccgtt acaggcgtta ttacagcggt tgctgatggg gattgtcgta 97680

tcggttgtag agcaacttat caaggtgtgg taactgccga agatagttct tatctcgcag 97740

tgaatgctgt ggtgattgtg atggactatg atttgacgca agaaactctt ccggcaggtg 97800

taagcgtaaa caggagtaac cgttatgcca gggcttctgg agatatcctt gtacctacag 97860

agagttctgc tcccgtagca cctttagaat atgttggtac aactccacaa ggtagacgtg 97920

ttgtagcagc caaccgtaca catatcttta tcaacaataa cttgacaaca gcttccccgt 97980

ggttgaagac gaacttgaca gtcacagcat caacaatcaa ctctcctgtt ggaactacgg 98040

ctaacactta caagttaaca ccttctacaa cttcaggaca acatatttta tcagcagtga 98100

atacagcttc tgatattaca gcaggtaagc actgggcggc atccgtattc ttgaagccag 98160

acgggatcac taaagtgaaa ctcaaattga cttcaggaga tgagattaac gaagggactt 98220

atgatcttgt agctggaaca tttacaggtg cagatgcaaa cataatcaat atggggaatg 98280

gttggtatcg tgtaattcta cacaaagtga cagttgccaa acaagcatct ataacctatg 98340

agattatagc tttaaatgca agtggatcag acactttcgc aggagatggt acggcaggtc 98400

ttattgcttg ggttccgcaa cttgagatgg gcgatatgac aaatccagtt tgggcgggga 98460

caccatttgt ttgttcttat gcacgagaca gttccagtgc tgctaccttc actattccta 98520

agaacggaca cgctggggtg gacatttacc acactgacgg tactgtgcaa cgagaggtct 98580

tcactggtac agctacaact tggatgttga cgttcaccgc cgcaagtgcg gattggggat 98640

cgaagttcat tacaggtttg aaatattacg actaataaaa aagggaaccc gcaaaggttc 98700

ccttttcttt tataaaactc tttataaatc aataaaatca gaacggaata tcgtcgtcga 98760

agtccattgg aggttcttct tgattttgtt gaggagcagg ttcttgctta ggctcctgct 98820

tcggttcttc cttcttaggc tctttcttgg cttcttcttt cttaggttgt tctgccgcac 98880

cacgaatggc attagctacc ttgtcagaaa gtgccagaac ttcacgagca ttttcaatca 98940

aatcttctac cgaaatagtc ttaccggaat gatctttaca ggcattcaga acagcgtgac 99000

caacagaagc ccccagatca taatcactga tatctacgcc acgttcttta gccacttcag 99060

ctttcagagt cacagtagca gactgaacca cacctgcaat atcaaacgct tcacctttaa 99120

caccattacg aatcaattcc agtgcaccgt ttacagcatg accagtctca agacctacgg 99180

tatcacgttt tttgaaggat tgtttgtttc cagtattacc tgaaacgcca gtcccaccag 99240

atttattttc tttcttctgg acaccttcag tagaaagaac agtgatgcga gattttttag 99300

tattgtagta agttttgtcg cctttggtat caactttgtc gatatcaata ctaacttcaa 99360

cacctttttc gatagtgatc caagtatcac catctttaac ctgaagattt tcgtaacctt 99420

ctttcagttc aatatcaccc aggctaatcc acgtcccttt tgcatcctgc ttggcaccct 99480

tttctttcag aacgagtgaa gcacggtgag tagaaacaat cgacttacca tcccattgga 99540

aggttttagg ctctgcgaat ttatccagtt taacgtagtc tacaacacca gttgcataag 99600

ttacagtttt gcctttacga tcagtttcag tattaattac ggacattatt atctcctcta 99660

tattcacatt gtttttgcac agaactcacg aagaagctct gcgttttctt tttctaaaag 99720

gtacttacct ttacctattc caactccaat ttctccattc ggaaaatgaa caaattgcac 99780

agaatgcata tcgagtttta ttgtaacagc atttccggct gtgtcaataa cttttcggat 99840

gttatcctgt aagctaataa attctttaac cgttatttca gtttcggata ggtcagagtc 99900

ttcgacacga gagaataact ttcggtgtcc acccttacct atcaggattt gaaagaagaa 99960

cgagttatcc agacctatta catctttttc aacaagtatt aaactgttca ggtattcgct 100020

gtattcagtt tcaacactta ctttcgaacg ttcagtcatt gattgtcatc ctatcataaa 100080

agattagtga aagtcaagct ttattaccaa ggaatatcgt catcagtatc ttcgaacttt 100140

ggtagagcct tatcttgcgt gcgacgctgc acatccaagt cacgtacaaa acgcatatgc 100200

gggaagcgat ccgcgatctt acggacgtgt ttcgcatcca ctttaacatc cttatgcacg 100260

aagcaaatat ttgttgcatc tgttaccaag aagtcaggat cgaagtaata ttctacacca 100320

tcaaacgaag ctttgcaaac agaaagatca aagtttttca ggatgttgct catgtagcat 100380

ggttccatga tcataaggtt gcaaacctga tcatcacgga taaactcaaa cacatgtttc 100440

agtccgatca tagactcata gttgtcaccc atttcctgct gacctaacac ggttacattg 100500

gtgcctttaa gatcctcttt gataaacgta attatctgat ctatgtttcg gtgtggctgg 100560

aatgatgtat agaagtccaa atctcgccct gcaatatcca acatatacca gtcacgaact 100620

gcacctcccg ccagaataga acgaggatca gcaatcttaa gaacagtaag gatctcacgg 100680

gcaagcgtaa cctggcttga tttagtcatg ggtacgctcc acagtaactt tgacaatatc 100740

tctgacgttg atttcatctt ccaggatttc aacaatacct tcttcataga gacggcaaca 100800

ttcttcccag acttcggctt cagtcattcc acctttaact ttatcagcaa cacctgtaac 100860

aacaccagca tcctgatcat caatcagaat tgaaacatta aaactaacta atttacgcat 100920

ttcttctcct tatacttcca agtggaaagt ttctttagat acgaagtcgt atccgccttg 100980

tttcagccaa tcatcataca cctcatcagc atgattttca atcataaagt ggcgagtagt 101040

aacttcatct ccaacaatac gaacgatctt ataacagatc ataataccgg aagtcattat 101100

tcgtcctcct ctaagtctat ttcattctga caatgtggac agaacccatc attatgcatg 101160

tgttgttcat aagacatgtc tttaccacaa gaccaacaac ggatatcccc atcatcccac 101220

tcacgatcct ccgcctcatc tgcgaactca tcgggtaggt aaggcatacc aaagcaatct 101280

aaatcgtaac gcatataccc tcctgtaaag ggggccgaag ccccatacca tcagtgacag 101340

gatgcccagg aactaccaac atcgtatcca gcagtaagct gaacatttag tttgtaatat 101400

tccccagctt tgataacagc aagagaagca agttctccgg cacgactgta agctgtaaac 101460

caaccacctt taggtgattc gtgaacgtta gcccaaatct gccctgtctc tttctcttta 101520

ttatgcttcc actcgttagc ttttgcaaga cattctgcat ctattttctt ctgctctttc 101580

tcatcatcca ctttaaccca gcctaaagat tctttagaga accatttaaa ttctaccagg 101640

ttacgagata cttcaagctg cgcttcatcg tggtaagcaa tcatttgctg aacgaagtct 101700

ttattcttcc agtcatcaac gaagaagtca acagctaaac cttcttcttc gatcatatcg 101760

tcataaatca ccattgcacg tttagcacag ataaccccgc cactttggaa taacgagttt 101820

aagatcgcat gtgcagatcg tgttggaact ttacgtccat caataccgag aacatactgc 101880

ttgccagtgt taacccagaa ggcatttaac ttctctttca gttgtgctaa cgggaaagcc 101940

gcttcccaga atgcatcaaa taccagttgt cctgtgtact tatcactacc aattgtctta 102000

gcaaccttgt ctgcctgtgc tccatatgta atcccgtatt taacagcttt agctggacta 102060

cgttcaaact tacgtccaat agtttctgtg atacgtcttg ccatcatggt atgaacatcg 102120

ttaggcttct ccaacaagag agcgttacag tattcatgag gctccggttc gtagcgccaa 102180

caataatgcg attcaatctt tgcttccaag gagtcaaagt cataaccgat ctggaagtaa 102240

cgcggtgttt ctaccccaaa caagttacgc atttcgtacc caaacaacga tgtaacgcga 102300

ggaacgttcg caacagagcg atgcttcata cgagatgttg cagcgtcaca cgtagccgct 102360

ggagtcgcta tacgaccatc ggcacgaact gctgccaaga aacctttatc atattcttga 102420

tcttcctcat cgtcttccca atctgcaccg ccacccaaaa tagagtttcg acgatgtttg 102480

tacgtcaggt actgcacaat ctgtgttgca tacgggaact tctcgcccaa tgcttcgaga 102540

ttaggacaaa tctctttctc ctgccccttc gtgaagcacg ggttggtcag cactttaaga 102600

ccacgacctt tccctttagc gagacgttca cggattctaa gttccagtgt ttcagcatca 102660

gcttgtaggt gttcgcaacg atcattacag aagttgcttt ccagagtctg tttgacataa 102720

gacagaatac ctttctctaa gtcgtcagga tctttcttga tcttcgaacc agatttaagc 102780

gtcaaatctt tgtctttata ttcagacgga acccaaccta aacctacaag ccagttttta 102840

atgtgtgtcg tatcattcag tgtagatgga atggtaggtt cacaaatagg ctcttcactt 102900

gagatcggta acttaaactc tttatctaaa acaagcagcc accaatcacc ttcacgttct 102960

tcaaggctac caccatgttt ctctgcaaac ctaatgatat ggtttgttgg ctcaccgttc 103020

ttcttgatct gaagtttcgg tggaataaaa tctttcagat aacccttagt cgctggctta 103080

ggtggaagaa taggttctac caacaagcgt agacgttcca tttctttgtc taagaattcc 103140

aggttctgtt cggcttgttg catgttgaac ttaaagccac ggtgttcttg acgggtgatg 103200

atatccgcaa tacgttgctc caagtcgaac ggctcttgcc acttcttata atctccccat 103260

tcttccatga ggtaatgata tactgctgta gttgctttgt tgtcgtaaat acagtagtac 103320

aacatatctg ctgcgaacgt cttgaatcgt tccccttctg gaatctcttt acggaaatcg 103380

atcttaacat ccccgccagc ttttttagag agagcattca agttgtgtcc aggtttacca 103440

tcaaccttgc gatcagggtt caatacttta ctaagaatca aggtgtcaat aatcttaact 103500

ttcgaatcac cccaagtatt gtatttaacg ctgtaattca gcccgtagta aagcttaact 103560

gccaagtggt catagttgat gccgttgtgg gctacagcca tttctacgag gtattcaggc 103620

ttatctgcaa acttgcctgt atggttgaca aaatctggaa aatctgccag agggaagtgc 103680

gtgtattctt gtgccacata ctccttcagt acataagtgt acttaccatc agactcttca 103740

taaggacgac catcgaaaat gtacttagga ccatcgtgaa aagcaatgat ctctcctgtt 103800

tcgtggtttt ccagacaaat acaatgcata ccaaaagtat ctttcaatcg ataagggctt 103860

gccaggtaat caattgtgtc attgttcagt aatgcggttg cttcaatatc ccatgtaaac 103920

ttactcatta gtcctccata ttatataact caacctgaag ggtatcacat tcctcaatca 103980

aaagtcaaga ggggcttgcg ccccttctga atcaatacgc gccttctgca ccacctgcat 104040

tatcagggtt agcaaaatac tgtgctttgt cgtgcagtgt ggcagtttta atatcgtaat 104100

agaattgtcc agcaggacca gtttccgaga actcacggtt tttcaagata taacaatgag 104160

ttgtgttgcg ctcaacaggg tcttctgcgg ctttatctcg ttccagtgcc agtgtaatac 104220

ctgcaccttt tgccaagaaa ctactaccca ttgcatcatc ttcagttaag tgctcattac 104280

cattgttcgc agctttctta acgtgggaga tcacgatgat cgtaatgccg tattctttca 104340

tgatgcgctt caaccacgtt gccaactctt cttgctcctg aacactcatc cccgacaaca 104400

gatccgagta tgggtcaatt accaggatgg taatgccaaa gtagatgatc atctccagca 104460

ctttctcctt gattgaatca atgtctgcac cacgatcatc acagacaaag aagcgaggag 104520

aaccatcttc acgaacgtag aagttattaa tagcttcctt gttctcttcc aggatcgcag 104580

ccttttcttc cttagtctta cgatgcaaag gaatatgcag gaaggaagaa agaaggttac 104640

gtgagtattt cttatacgtt gcctccaggg atagaatccc caacacatgt tctggatgag 104700

ccacgataat gtgtttagtg atttcattca ccatcaatgt cttaccgata ctcgttttag 104760

ccagaataac tgtgatctct tcttctacca gaccaccacc caacatgttc gcagcagtac 104820

gcatgaacgg agggagggtg atcacttgtg catccagaca gtccattgca gcttcataaa 104880

gttcgttcga agcataaaca cccgctgggg tataaagttt tgcaccccaa taagccttca 104940

caaagtcttt tgctttatcc tgcaccaagt attcattagc atctttcagt tccatcttca 105000

tcaggtaaac tttattacga ggcaacacct tcacaatgtt ctcaaaagct tctttacctg 105060

ccttgtcatt atccatgcag acgatgatct tcttgaactt ctcaaagaaa gggtactgtg 105120

cctgtacctg tttgtacata ctggtttcac cagtcgtacc actaactacg gctggtaatt 105180

catatttcct gtcaagacct tctgccaaca tttgatatgc tgccaaggca tcatgttcac 105240

caccagtaat gattacagtg tgggaacgct caaggaaacg aacttgtcca aacaaatcta 105300

catctttacc tacttgacca atgggcttgg agaagtcttt aggatggata cgacgcttaa 105360

aacctaccag ttctttaccg atagttgttg gatacaaagt atgttccaca acaataccgg 105420

attcttcttg tttgtactgg taacgaacac cgtagaattt agaatattct tccttgatcc 105480

cacggtagcc tcgtgggtta gtgcctgttt cttctacaat cttacgatga atctctttat 105540

taaattccga acccacaata tcctcctctt cttctaaaac ttcaccattc tctcgtaacc 105600

attcaacgct ggggattgtg tactcacacg cgaagcattt tgctccttta tgctcaccgt 105660

tagaatccaa cccgtatacc attaaattgt tacgtgagcg atccttgcca ttcttcatac 105720

agcgtggaca accagtatgt ccttccctgc taagatcaac atcaaatcca tatttcgtta 105780

taatgtgtgt cacaatcctc tcctgttaaa atgattcgtt agtttccatc atgtactcgg 105840

cttctaatgc ctccatatgt tttttgaatt ggtatgcaat ttctgtcacg gtcacgttaa 105900

tgtcttccgc atcaagctgt tccagacctg catagaaacg gataccatca atcagtaagc 105960

cgccgtgata atcccaatca ctttcccgtg aatgtggatc aggttcttcg tagtaataat 106020

caacttccac cacaacatta aaaccacttt tcgtagtgta ctcaaaatca aaagtattca 106080

gcattatagc tcctcaaata ataatctacg gcgttcattc agcacatcaa tgtgataatg 106140

cttacgaaca tatttagcta aagccaatcc cttctcgatc cccacatcag gttcttggat 106200

caggtaatta acaatatctt cccattcact tcttgattct gcaaagaaca gtaaatcctt 106260

aaactcttct gtgaagtaat tctcacgagg agaacagacc aaaggcagac ccttggcacc 106320

agcctccaga atcttaaggt tacttttggc gttattaaac atattatcgg ttaaaggtgc 106380

aagcattatt tgatgaccat cgtaagattc catatagttt tcatatggtc tgacagcctc 106440

ataattgcag cttggagcca ctttttcacg tatgtcagtc cattgctgac tacttaacac 106500

ttcactatcc ctacggaagc cgcagatggt taaatccctt ccaaaattcg gcagcagagc 106560

caaatcttgt ttgtgtgttt cacttccagc ccatacgatt ggtgatttgg agtagcgatc 106620

acgagttaac gtaaactgat cctcgtcaaa cggtaaacca tttgggataa tatgcacgtt 106680

aggattgtac tgggacaatt ccacctggag agcaggggtt gttgtcgtta caagatcact 106740

gcgttcgagg aaccattgca agtcttctcg aatacctgca tcaaaaatgt cttccagcat 106800

atggccttgg ggaatgttta aagagtcatc caaatccatt accatcttaa agcccatgct 106860

cttaagggct atgaagcctt gtttcccttt cgtagggaca ccgttaaaga catatacgtt 106920

ctgtgacggt tgggccgaga actgccttgt gtaaggcagc attactcggt gataatcaca 106980

gacactatat tcttttccac cacgattgtc aagaataatt tgtcccataa acacctacag 107040

actttcgatc acttgacgac gcaattcgtt tgcatccttc aagttgtagt gagtacgaac 107100

atgttcagcc aagaacagtc cacgatcttc acggtagttt ggattacgaa gtagttttac 107160

aacttcatag tgccactctg ccttgctatc tgcgaaggtt actgcacggg catcgatcgg 107220

gttgtagtaa ggtaatacct tagaacaaac gattggaata cctttagcac cagcttctaa 107280

tactttcagg ttgctcttgc aatggttgaa gtcattatct actaacggag ctacagccat 107340

agcatgacca tcgtatgcag acatataatg attcaaagaa tctacagcag gtttatactg 107400

cgcattaggc aaactacggc ggatcttaac ccactcttgc cctgtcactg tgctgctcgc 107460

ttcataccct gcgatagaca acaggctatc atcaaaagtg tttgaaagca aacgtaaatc 107520

cggttcatga ctcgcacctc cagcccaaat gataggcgta cccgaagtac gatcatttga 107580

gagagtgaac tgatcatcgt cgaacggtag agcatttcgg atcacaacaa cattagagtt 107640

taagtgacga attttatatg ccaggtattc tgttgtcacg ataaccacat ctgccaattt 107700

aaccatttcc acaatggtgc gggtgtttgg cgcgtacaga tgcgccaggt agtgttctgg 107760

attcaattga tagaaatcat ccagatctac aacaatctta acaccttggc gcttaagttt 107820

ggctacttca tcagcaccct tcgagaagat gcggttaaag accagtacat cagtacgtgg 107880

cttgatctct atgttagaaa atggcatgac aatgcgatgg taatcacaac cactgttgaa 107940

tttgttgtca caaagtaggg ttggtttctt atttaacgcc atatccttca gcctcttcaa 108000

aagagatgtg tgttttgcca ttattaatat cctccaaaga cagtttgtat tcttcccaat 108060

tgatcccgat tgccgattct ttctcttctt ttgtcagatt agcaaaaggt aaagggttat 108120

caccgaaaac atcgtcgggg tgaatgtacg ccataatgcg catcggacaa ggtacgccct 108180

ggagtccttt attcaaacct gaagctttca gacgattagt ccattctgca tcttcgaaac 108240

catatccgtg tgtataagga cggaaataac ccatcgtctc aacagttgca cgagaataca 108300

ttgcaaactg acacatagcc aattcccaga agactacttc tcccgaacaa ccgatcacag 108360

tatctttgaa gtattctggc ataccgaaga agtcccaacc attctcgaca gcctgtttaa 108420

cgaagtattc ttcccaacca cgtttaactg gatagcaatc atcgtcgaaa ataaaccagt 108480

agtcaaaacc ttcatcgtag aactgtttca tcaattggtt gcgagcataa gctacacctt 108540

tacgatcggg gtcgtgaaac actttgaagt ctacaccagg tgcaaccatg tagtctttaa 108600

gtggacgaac acctacagtg ataacgccaa ccccaatttt aatattttcc atctttcccc 108660

ctaaatagtt ttccggtgat catgacaatg cataaggtgt gatattgccc gttgaggatc 108720

tttttgacct aaacggtaaa tgtggaagag atacatccca cgcatcaatc ctaacctttt 108780

tcctcgttca tgtaaagtgt ttgagaagat tatatcgaac tggatgctac gttcttcaaa 108840

agggatctcc ttccataatg atttcctgaa caatagaaag aaacctgcta agggagtgcc 108900

ttgtggaact tcttcaatct catctccata gtaatcacga gcaactttgg catgagaaat 108960

atggttcaga atgttaggat catcatcaat ctttccaccg agaagttgat aactactacc 109020

taaacgatta caagacgcac cgatcacatc aaacggtgga ttggttgcta ccaaatcttc 109080

caacatagaa ggttggtctg gcagtaagaa catggtatct atatcacgta aacagaccca 109140

actatcttca tgaagaacgg caatagcatc attaattgct ttgccgatat ctccagtcag 109200

atagggggta atataataaa tcatactaac cccttactta gaatccacta ccattccgcg 109260

tttaaccatt gcttgacgaa tctcaagcac acgatcccgt gcttccatat attccttgaa 109320

ggagtcttgg agatgatcca aagccacatc ccgcatttta atcagtttgt cgtttgttac 109380

gttctcaaga gaaagttccc ctgtattatt tatcccattc gttttcatca acaaccccct 109440

taatctgaac agcacaagct ttacaaatgc aactaccgtt gccctttgac agttcgatat 109500

acggctcatt accgtcttcg ccctttcttg aatcgtagat tacactattg aaacaaacaa 109560

cgcaaacact tttcaatatt gtatatttct ctactttctt ccgagtacgg aagtttacct 109620

caatgatatc tgccattagt gaatcacccc caaaatgttg ttttgtgtca taaccattga 109680

agattcaata gcatcgtgac ttcttgcaaa acgctcccac agaacagcat ctactttaag 109740

agctatgcaa ggataaatac tgtcgttgat caactccacg gaatatttat caacatcgct 109800

ttcgtcttcc atgaagaggg tagcgtaagc ttccaaacct ttacgaatgt agtacacatc 109860

ttcttcggtt aaaccgataa cgagataact cgcaacaaca acagtattat cttctcgcat 109920

attagctcct tgtcggtgag tagaactgat gttctttata acgatacact aagaccatat 109980

cttttgtcca gtgtgcacgg gtttttactt tcttaaaaaa tgtcgcacct tttgtcatgt 110040

cctgggcttc tctaagtgta gggaagtttg tatccaggta tataagtcgt tttgcgatag 110100

ctttagcttc tttccatcgc tcggcttcgg taacgttaaa cttcttcctg taagaaaatt 110160

gtcctcgttg gaagagaata tctttaatat tatcagggaa gtaatcatcc cctttcctgt 110220

tcaatactac gttacctaca gcatacattc cacgtttacc ctgattacga ctttcagcgt 110280

aaattgcaca agctaagaga ttttctttag tatcttgctt tgcacatttg tgaactactt 110340

tagcagtctt tacgtttgca gcttcggcgg ttgccgtgac aggtgtaaat gtaaaagcag 110400

ttaaggatag taacgcaact gttacaaagt tttttatcag caaattttgc ctccttttgg 110460

gtgaatgaac taccagtata acacatgaga ggcgtttttg caatggggga tttctccccc 110520

aacaacattt cagttaaatc ttacgggcaa tcataccatt ctttctgacc accgaaggcc 110580

ataaccacat cacagatatc catacctgct ttaccaacac cgtaagcttt cagtttccat 110640

tcgccgtctt cacgaaccag ggaagctacg tggcagatcg tgttaccagc tacttcagga 110700

gccaggaagt cgaagtccag gatctcaacg ttacgctcgg catcaaaaat cttcagcata 110760

ccttcgctgg catggcccag acgtttctga cccgtttcat catcaatagt caggacaaaa 110820

gcaatctctt cggctttatc ggagatctta gccaaggtta catcgatctg ttccatttga 110880

ccagtaccgt cacgaacatc atcccctgcg aatacagaac gttctggatc aaacttgtta 110940

gcataacaaa ccagatgacg tgcagacagc atttcagggt tgccagaagc gttgtgtcgg 111000

catacaagtg cgcttacatc cagatccagt ttagagtttg atttccagga gaactccaga 111060

cgcagttctt tcagtgccgg agcaactttc ttcagggaaa tcttgtcagt ggaacctttt 111120

ttcagggaaa ttttatcgct cattatatgt ctcctctatt atttaagttt aatttcgtta 111180

actgttaact ctacctgcac caactctgcc gtatggtgta ttgaacacaa cattgcattg 111240

taattcattt ctaccgcatt gtatgggtgc ttggtgtctt cgtctcgccg caataccata 111300

taaacgatga ctgcttctgg gtcaagaccg tcttcatcgt aggcgctttt cagaacaaac 111360

aaatcccgat gtgtaagatt tgcaatgcga caagtctgaa tctctggttt gctatctttc 111420

ttaatggcga aatttaacat cttgtcctcc tgtcgtgatt tctggtttgt tgatacgcat 111480

tataacagct ttatttgctt tgtcaatcca ttcttcataa aaaggatact gatggatctc 111540

aacacggagt ttaagttctt ccaaagaaag agtcccaaag taatctttaa gacccttcat 111600

gatgaaagct tctctctcgc gcatcacttg caacactgtt tgagagatca gcgtgtcgat 111660

catttcttag caccaacctt ttcaacccac tcaccgaact cttcagacaa gagtttatcg 111720

taaagctgct cgtctgtcaa ggaaagatcg ttcagaccaa tgaatgctac gttgtcgtaa 111780

gcttcagcca aatctttcag gaaagagaaa cgagcactac caacacccac catgaaccag 111840

tagataggtt tacccttgtt cttttccagt acggagagta cacggctcgg atcatcgttc 111900

tcaccatctg tctggaagaa cacaactgcc ggagtaacgt ctgcaccagg cacagcaacg 111960

gctacagttt tttctacaac agcttctaca gtttcagttt tacccagtag acggttgaag 112020

aaacctttct taggaacttc tttggttaca ggagtttcta cagtttcata tttagtggtg 112080

ctaccgaagt aggaatcgta aatatcctgc ataacaggtg catagcttgt gccaccctgg 112140

actgttacac gaccattaat gcatttacca acgtaatcat catacacatc agcagttgct 112200

ggagtgattt cacgagagct attgttgaaa gcccacatat cgatctgcgc gttatcatcg 112260

aacagcatac caaacggaag cagcttaccc acaaactcac ttacagtacc gttacgatac 112320

aggctggaca tgctaccgga gatatccagt gcgcttgcaa cgcgcagaac gatcttttga 112380

tcttcgttga caattttctt tttcttcagt gaaatggtca gggtgtcttg acgtttcttc 112440

aggctaattt tattggacat attatttctc ctctgtaagt gtaatatcgg taactactaa 112500

acgaactgca accatcggca catctgggga tacggtatgg acactccctc ttgatgaact 112560

attcaaacaa acagcagtcg cgccgccgct tttaagaaat actaagtttg caaacgtatc 112620

caaaccattt tcttcatatg ctgaataata catgaaagtt tcaccaatcc ccaactcttc 112680

gaaacgaact gctacatctt ggggttttga atcttttatt ttaaaatcaa acattccctc 112740

ctcctttacc agttttgaag cccaaatcgt aagcttcttt gatgattttt tgtaactcgc 112800

ttgtaggcag tgttacaaga ggatcatctt cttcaatttc cattttgtcg tcttctgttt 112860

cagtaaggac attatgcact ttatgataag ggttgtcaac acgtttgaaa atattatttt 112920

cggtgctgta gaaataatcc tcttcttctc cgtttcccca accttgatta ggatcatcct 112980

cggatcgttt tgtagggact tctcctgtaa agtgttctgc taaaaccata gcaataccgt 113040

ctccacggtt tacagagaac ataagatcac aaacgtcgct ccaaggtata gtccattcaa 113100

aactttctct gttcccgtga gtgtcctgtt tactggagaa gacacgccca taactatcat 113160

aattgccgta catctcttca atgaccttcc cgtctttcag cagaaagaga taacaaggag 113220

atccatcaaa agatgtggag agggcagctt tgcccgactc cttacacaga aaactaaagc 113280

aacccattaa acaccttcct taaggttgtc aatggcaaca gttgtttcac tggactcgtc 113340

atactcttta tggagacgaa ggaaatcttc tggcccactc caatcatgat cttgagtctt 113400

ccatccatca atgaaggcat cttccatgat tgtgatcagt tcctggcgag ttaactgaat 113460

aatatcgggg ttcttaacaa cctccacacg gaaacctggt tcttcgtatt ctcccaaggt 113520

gataatagat accaactcat cagattcaca gagataataa cctttaagga agcaagaacc 113580

atcttcgtct gtgataactt cataaacttc accgcttgta agattgatga aaagagtcgg 113640

atctgttact gttaatttca agtttacgtt tgtgtgttta aacatcttct tcctccccta 113700

aaatatggaa cccgtaatta caatagtcct gaaatgtttc ttttgcaaga tcttcgatat 113760

cttcttgaga catatcgtct tcaatttcga tatccacaac ctcattgcat ccacaccaag 113820

cagtttcaat attcactcgt aaagttttca ttcttcctcc tcgttaaatt ctacgtctga 113880

aagaatctca aaaccaaaac tattacaagc ttcataagaa taatctttcc gtaaacttgc 113940

taagatgttt ggaggctgta cgctgcacat aggtgcgaat cgacgttcgt catcatagcg 114000

tacaccaaaa taattttcaa ttacatagat acggattctc atttaccaag ccccttctta 114060

acagcatcaa ccatcccatc ttcgagagat tgaacttcgt tgatgtaatc aacacgggca 114120

gcacgggctt ccgtattgat gcggttagtt tcttccaaca tcttaactaa gttttcctga 114180

tcgtgacgca gggtttcaat atccaacact tggcggttag ccagtgctgc tgcttcgatc 114240

tggttctgtg tggcaagatc gctacccagt ttgattgcca ggttgaactc gtccagaacg 114300

ttgttctgtg tttctgctgc tttcttctgg cgcagacttt caatatacat cgagaagttt 114360

gccgagtaag caggaatcac attgttcaca atattacgag cagaacgcat gaggctggca 114420

gatactttct gcatacccaa tacacgcggc cccatttgca cgcacagcaa gcgtaatgcc 114480

ttcaagtcag caatacgtat ctccaaaaga tcaagcgtgt cacggacgcg ttgacgggct 114540

tctacgtcat cttccggtgt ggcttcaaaa gtagccagtt cttgctcata agctgctgtg 114600

agtaattcca tatcacgatt caggcttgcg gcgtattgtc cgatcgcctg gtgcagcttc 114660

tccaggtaca ccagagagtc ttcttcttta cggatatcca tttgaagatt cttaacgagt 114720

tcttcgatac ggttgttcac gttatcgaac tcaccgaaga gatcttcttt catccccatc 114780

agtttcttga tgagtttacc tacaccttta ctctgcttaa tggaggcagg atcaagctgt 114840

ttggcttgtt taatcaaggc ggtaaggcgt tgactcattt cgtcaccttg attgctacgt 114900

tggtgtttca gcacttcagc gttcagagaa cggagagtta aacctgcttc tgcacctact 114960

tcttcaattt ctttacgaga cattgttggc agcgtaggca cagcttttgg cgctgctgga 115020

gcatacaccg gacttgcatc caagacactc ggagcgaaca cagacttgct tgcgtctgta 115080

gtagcagtca gtacgctcgt ttcagatttc ttttgaccac cagttgccgc tttcaaaaat 115140

ggacttgtca ttcttcattc tcctcttcta tttctcttgt gtaaggtgaa acaccatatt 115200

ctttagctac tttaacccaa tcttcccatt tatacaacca tatttttcct tctgttcctt 115260

ctctatcact cttcaaccaa atatgcggtt gtttccctgg agtcatacgg ttaggtttac 115320

gattataccc tctaacacat cttgtggcaa atctatctac caaaacaaga acatatttgt 115380

actcatggtt taacggttct ttggaagtag ctttaggatt ggctcccaac tctgctgcca 115440

gcttttgcca gtgtggtcca tgacgactga acttacccat acaaccgtcg atcgcatgag 115500

caatctcatg cttcattgtg tccactatca tatcccttgt ggagttttta tgcaagtgcc 115560

aatttacaac aacaatcttt tctcggtagt tgcaccaacc gaaagagtta ttcactttgt 115620

tcctaacgca agccgtatat ccagcttcac gtagcatgtt gtagtaaaca gggaaatcct 115680

ccttgatctc ctgcatcact tcccaccaca agagttttaa atctctctcg gtattcatgc 115740

attacacctt gaagttaaag cttgttgtgg tagtttcgaa atctggtgcc gttaagtgtg 115800

cactgaaatc agagatttga ttaataatga aactcttatg tgcacctaaa acaacaacac 115860

ttttaatatc ttctacagac atttcccaca tattggaaaa acggataaaa gtttctcctt 115920

cttctgcaac tgtgaagaac ataaggtttc cgccagcagt gtaacccact tctttaaaac 115980

ctttgatcgt atattgctca ccgctggtaa agaaaactgt cgccttattt acagtgcgct 116040

tacgctctgg aacacctttg gcacgtttac ctttaacctt tttaactttt ttggctgcaa 116100

gaacttcttt cttctttttc tgttcagcta cggcgtacat tacgctcatg aaatctttag 116160

gagacaacag gagataatcc tgctctcctg tcaattcaga aacatcatag tttacagcat 116220

ccacataatc ggaaaaacca ttacttttct ctgctacaaa gtgagggaaa ttatcccctg 116280

cgaaaaggtt agatctcttt acatccacat acgtatcttc cataaaagca gagaatcctt 116340

caaacccagg agatttttta agattgtcaa tatttactgc aatcttatac tcgtctgcca 116400

acgggcggaa aggttgctga aagcagctac gcatctctgc atactgaaga actccgttag 116460

taataccttc gaaaacaatt tcattacatt cttctcttat ttctttcact cggaagacgt 116520

tcttgttaac ttctaatgct tctggggaat aacccgtacc agataagaaa tacagatctc 116580

caactacagg taatggctga atatttttca tattttctcc tcatcttaag ttattaagta 116640

gtgaccacca atcaaaatct tctcttgact tctggtgtgg agtttcttct ttctgcgtat 116700

taacacaggt gtgtttgaaa tctccagaga agcagtaaat ctgctccccg tctttgtata 116760

gggatacttt actaccattg atcacgtttg tcaacacaaa aagtatgacg aacaaaccta 116820

ttgcaaaaca aatattttca aacacaaccc aaacaacttt cattacagaa atacctcgtc 116880

atctgtctca ttatcccaga agaatagatc ccaagtgtca gagttatccc atttagcata 116940

aggagaggtc cagaaaccat ataccttaat ttctgtaata atatcatcga catttgcact 117000

tgctgcttgt agttcgtaac gcagaactgg ggtgactgta ccctcacctg aaccacaaca 117060

agcttcaaca ttaggtggaa gatccatagg aggtgtctgt ttaacttcta cataagcata 117120

aggaaaaacc tcttgaatcg cagcttctac ctcttggatt gtgccagaaa gacaccaaac 117180

atattcctga acatcatcaa aatatacaga cataattcct cctatgttag taagaaacct 117240

cccgaaggag gttcattggt attaagcggt agcttcttct ttttcaggga agatgaaact 117300

tgcaatcacg cccagtgtca gagtaccaag cacgatatac atgctgatcg tcgggtcaat 117360

atgcgctggc aggaagctca cttttgcgtg cagcgtttct gcaaacgggt gatacatcag 117420

tttcacacca acaaacacca ggaccgcgat aactgctttc tccaggtgga ccagatactt 117480

cagggccaca ctaagaacaa agaacagagc acgtaagcct aagattgcac agagcatcga 117540

agagaataca agcagtggtt cttgtgtcac tgcaattaca gccggaaccg agtctactgc 117600

aaagatcaca tctaccattt ccatcacgaa cacacaaacc agagccggag ttgctacacg 117660

aacaccattc ttaatcgtga agaacttctc accatccatc tgatccgata ctgggaacag 117720

tttacgaacg acgcgggtag cccaatgttt agagtaatca actgctgcgt catcgtcttg 117780

tttaaacaac atcttcgccg cacaaaaaag aatcacaata ccgaagaaaa tattaacata 117840

ccatccgata cttagcagat aagtccctgc cgctacaaac acacctcgga agacaatcgc 117900

acctgcaata ccccagagta gaattttgtg ttgtaagtgt gtgctcttaa tcccgaaact 117960

ggagaacact gctacaaaca ctaccaagtt atctaccgag agcgacttct ctaacacata 118020

cccgctcaag aacaagtttg caaaagctgc gccgtgctgg aagtatacaa aaccatagta 118080

ggctaaagac acaccaatcc agaatactga ccaagccaca gcggatttga atgagatctc 118140

cttgttctca cggtgcccga aaaaatccac cgcaatagac agtactaaga tgcctaccag 118200

aaccactaca tcaataatgg ggaaaccaag ttgcattatt ttctcctttc tctttgcggg 118260

gaaccactcc ccgcctcatg tgatacatct tactctattc taaaaccttg tcaacactta 118320

ttttgaagaa ctggaagaaa agattccacg tagcactgca ataatgaacg cgacacacac 118380

tacagtccag aatgtcactg gaacagtgat atctgtatga cccatagctt caacaacata 118440

gttgtaagcc agtaacaata accacgctgg gatcaggccc aacagaaaag caacagcaaa 118500

cacaataacc actgcaagaa aacctgctaa aacgcccata atatttctcc tcttaaagtt 118560

tataaatagt cacatcgtga cctgttttta ctaaagtttc ttcaattatc acttcgatga 118620

tagcccaatt acctccggcg tgacccgctc cgatcatagg taaaccaatc ttacattttt 118680

ggtcacggag aagcaagtcg ttagccatag ctattagtgc atcataaagt gcatcataat 118740

ccaactgacg acctttagta ttgtatccat aacgatactg cccgtataga ttatatacca 118800

agggatctga ttgttcgtcg ggtactccta aactaaaaga acctaactta ccctgatctc 118860

ctgactttgt acctaaatca gcagcatacg cataaggaaa tgctcgttta atttgcggag 118920

caatgccttt acccatgtta cacatacagt tacaacaatg agctataacg ttcacttccc 118980

cattcttggc tgcatcgatc agattgccag taacaacctt caacattttt cctccttaaa 119040

ggatatgttg gttaaagtag cgattgagga acacaaagtt ttcagagtac ttctcaatct 119100

tatcgtaacc ttcaccaaga cacccaaaga acagtgcttc gacgtaatga tgcggcagac 119160

gggagagaat gattttaaac tcttccggtg cttgtcccat caaatacttg ataggacgaa 119220

ccaggcactg tgcatcacgt tcttcgttaa tcatcttaac gaaagcatct gtcgtgttaa 119280

tgtaattgaa gttcagttta gcttctgtat ctgcttgatt accagtatac tttgcaaaat 119340

acaggaagta aacatcattc gtcaccttgt tttcatccag gatctcaatc agtttatctt 119400

catgagttcc cgtgatgttc acatcgtcaa tgaaaatcag cttcttgttg ccccagaaat 119460

ccttgttgca atagaaggta tctccatcaa tcaacttctt acgactggct ttatccagga 119520

aaccataatc gttaatgtaa ctcaccttgc gatgaatggt atccaactct actgcacggc 119580

tacccttgtg attcaacagc aacaggttca agaagttgat gaactgattg gtcatcatcg 119640

ttgctgcgtt atgcacatga ttgtaaggtg aagggatcac cacaaactct tcttccggca 119700

tattcttgac taccaagtca tagaaagaga ctgctaatgt tttccccata cggcgtgccg 119760

cctggttgct accaaacttc agtttggaat aatcacgaat atcaaagcga gcagtttcca 119820

gattatcaaa tttgtgagca aagatttgag tttgattacg cattaacaag ttctccttta 119880

gcaagtttat ctaacaagtc gttagggttc ttaatatgac agaagttaaa tcctatgtca 119940

acacattttc catcatattc ttcactatca cctacgtgca acacttcctg ccctaccaaa 120000

gggattggag aataagtgat cgtgcgagca aagaatcgcg gatctggttt acaaagtaaa 120060

gcttcatccg agaaatgtgt gaactcaaaa caattccaac tttcaaactt acaggcacgt 120120

cgcaagatat ctcctgatat gaagttggta ttgctcttaa tgctcaaatc ataacgcccc 120180

gaaagataca gcaagtgcat cggtagatcc acagctacat gaggtggaaa cttaaggaac 120240

cattgttcac actcgaaacg aagctttgca gacttcaaag gtgttatccc aagttcgtaa 120300

ccaagtcttt cccaggcgta tgctgcacac tcggctgttc cctgaagtgc ggcattgtcc 120360

agttcctttt tgatcttttt gtaagctgct cttgcttcta cttccgtaat atccccgtat 120420

tttgcgatag tccacgctcg catatctgcg taattggggt ttgctgtgat cagtgtgttc 120480

catacatcaa aagaaatgtg tttgattgtc atattaagcc caccatgcgt ctaagttaag 120540

attactgtct actaccactt gttctacatg gtagcccttt atttcaacat tatgtgccag 120600

aagcatatca taagctttct tgaactgcaa cttatcttct tcggtataca gacgggcatt 120660

atagaaacga taatccacct cttctgcatt agaagagata cggaagaagc cagtgtcaat 120720

ctccggcata gaagccggag tcttgatgtg tacctctccg tagtgctctt tgcttaggtt 120780

catctgcatt acatgttcta catccttgaa catttgttcc agtttatagc gtaatacttt 120840

gaaaccagca gcttttaact ttgccacaca ttccaacatt agtcctgtgt caatgccacc 120900

ctgatactta gttaacatac gatcgcgttg tgattgtgtg tcacgatgta gatcgatagt 120960

tgtgcatttt gcaccgataa tcttcgctgc tcgcataagc tcttcactac ttacctcggc 121020

taaggtcaga tgaaagtgtg ctttccaaac tttgtctact ggtacaccaa aaacttcatt 121080

accagcgcga ggcatattaa cgatcagttg ttgcattgtt agtctccttt agaagggcag 121140

tcgttgaagt tcgtcaccgc cgatatccag tagattagca atatcccgat cagaaagcaa 121200

ctcttttctt tgcaaaatat caatcagggt acaaataacc tcacgagtgt ctcgatcact 121260

atccataatc agcttcccag ccgtccctac ttcgtttttc actgcaaaat acgtaaccat 121320

attatctcct gtattaatct gcttgccaaa taccattctt cttgctccat aatctctgat 121380

tatcagaacc tctgaatggt ttttctgttg gttgatttaa atcatattta ccatccatga 121440

ttgtatccac aacatccttg atgtgggaag gaatgtcttc aaacaaatat cctgtataaa 121500

ccatcacatc cttatcttcc ccgtacactt ccttaattcg gagtatcaac ctataaacct 121560

catcaatatt ctgtgggtct aaaggttctc cccctaagat tgttacacct ttaatgaaag 121620

gtcgtgccag atctgctaac acacgttcta catctgcttc tgtgaaagct gtgccataag 121680

agtatgacca ggctttctga ttgaaacatc ctctgcatcc gtggctacat ccactaaccc 121740

ataaagagca tctcacccct tctccgttaa gcatgtctgt tgcttcatag cctgaacaat 121800

tcaaagatgt ttctcccttt gtaacacttc ctgcattttc cctttgttgt aagggcgcga 121860

gttaggggcg gatagatacc cagaaacacg acgaataacg gagatagttt cttcttcatt 121920

attcccgcac gaagggcatt caaaaccttt tgctgtagct ttaaactccc catgaaagtt 121980

gcatttgaaa cattgatcca ctggttgatt gatgccaaag taatggatgc gctcataagc 122040

ataatcgacc aaagcttcta aagcttccaa gttgttcttc aggttgggag tctctacata 122100

actaatattc ccgccattac tgatttgagc aaaacctgtt tcgtagtccc acttctcaaa 122160

aggatttgta acctcccaaa caggttgatg gaacgagttg gtcaggtagt cccgtacatg 122220

cagattagga taatttttat ttaaagctgt ggcaaacttg tagcacaacg attccgcagg 122280

agtgccatac aaactgaaag gcagattaga caattcttta aactctgtac aagtgtcttt 122340

catgtattgc agaatagctt tgcccatctc tttcttattt gcttcttcag agtataagat 122400

ctccaaagct tcggataatc ctacataccc aatactgatt gaagcatacc caccctcaaa 122460

caaaacgcca atctcatcgt cagcattgag acgtgctaaa gccccttcca taaacaggat 122520

agggttttct gatgcgcgtg tacggcgaag gcgttccacc cgaagcatat gagcatcgta 122580

agccagacgc atatgttcgt caagtagatc agagaaacaa tatccttctg tttttgcttt 122640

agctgcaatc aatggtaagt ttattgacac aactcctaaa ttaaaacgtc catcgtactt 122700

ctctccttgt tctgtagcgt aataagacag gaacgaacgg caccccatac tggtaacatt 122760

tcctttcgta gagcctgtca cttccttgtt aagaggtaca gaaatgaagt caggatagat 122820

tcgatctgca caacactcta aagcttgttt tttcagatcg taattaggat ctccagttgc 122880

catgttaaca ccttcctcca ggaagaacac aactttaggg aacacaggtg tgacatgatc 122940

tttccctaac ccttcactgt gtaccttaag atagttctta gtgatcatcc tcccaaaaac 123000

tgttgtgtct aatcccatac tgatagtgat gaaaggagat tgtccattga cgctcatcag 123060

tgtattcacc tggtacaaca atgtctgcat agcatcatat acagccttat ctgttgctgc 123120

taaagcttcg ttacagtctg ggtatccgta acgtagctct gtttctctta aattcttaag 123180

gtatgtagct tcaacatact gacttaaccc ttggtcgata tgagcacaag tttgtccccc 123240

gtactggctt gaagcaacag cttgaatgat ttgcgtcaat actgtggtgg ctacaccaat 123300

ggacttaggt ttctcaatat acgcagcacc gattttaaag ccattctcca acatgtcttg 123360

atagtttacc agacaacaat tcgtaagagg ggacaataga taatcaagat cgtggatatg 123420

cccaaaacct tctgtatgcc attctgcgag gttttttggt aatatctgag tcattgccat 123480

gtgcttactt aaaatccccg ccagtaagtc cctatgtgtg ttaacgaggt gggagggttt 123540

gtttgcgttc tctcgtgtat atgtgtcaca agttttatcc aaaaaagaaa atacgtcctg 123600

atataaatta tgcatatgtg ttccttattt aatagattga gattctattc taccatttat 123660

ccatagaaag actttgttca ggatcaaaaa aggcgcactt agcgccttct tatttattat 123720

ttagaaagga actgtcgtcc catcagctta ccacctttaa cggcagtacc atcatgacct 123780

tggtaatccc aagaaccgtc aatgttagct aagaagttat cacggtgtcc attctcttca 123840

atcaccaccg cgagaagcag gttgctgtcg atctcggaaa caacagactc aacaatacct 123900

tctgcaatat tacgcgcata ttggatatac actttctcgc ctgtaacctg cacactgtcc 123960

acattcttaa acatgtaggt ttgttgtgtg gtgtagagaa tacgtacaat tggcttctta 124020

cctacaggag gtttttggcg agttttgcga taacgtggct tcggtgcata taccggagca 124080

accacctcat cttgaacaac ctccatttta gctacacggg gaacaatcag gtatttcacc 124140

agatcccaat catgaggatg gttctcatct actctccaac ccatatcatc ggctgggcct 124200

gtgaaagttt gcccgttacg gcagacgatc ttcacgtaag gattaccctc tggagggaag 124260

ccagggttcg gaaggtattc ttccacatat tcttcaactt caaaagtatt ctcttccact 124320

tcttctcctt cttttgttaa ttcagcaatg atatcttcca atgatttctc ggtattgtca 124380

atacctaaat gcttcaaaaa cagtttgata aattcgtcaa cacctatttc taatccttgt 124440

ggactgttgt gaccatcaat ccccgactta gaactataga aagagtagtg ttcagacaga 124500

ccacgactgt acatcagaaa atgttcagca tctttccaat acccttcacc agtcaccagg 124560

gtgaaaatct ttaaaagaga ttccatttga cttgtggaaa tctttctggt agatacgcac 124620

acttcgtgaa tagatcgccc tgccacttta gctaaaataa gatctaacat ttcttctcca 124680

tctatgattt ctggttttgt ccctaagcgt ggaatatttt cataggagat agaatccacc 124740

cctaaaactt cagagaaaat aacaacgtca cgcccaccat gcccttcttc ccaagctttt 124800

tggagttgat cggcagtcca accattacca taaagcgcgg ctacagccgt atataaacgg 124860

gagttcccat catacagacg acttgctgcc atacgcagcg tatccttcat acagtagtag 124920

ataggcttcg aatcacacca ggctgtctcc aaaagcagat caatgaaatg ttcggtagtg 124980

gtaacagaag taacacctgc tggcagataa tcctcggagt gtgtagcatc aataccatta 125040

gatggatcaa agaacaaaaa ccctgtctct gcgcctacat cattacgaaa atcttcttcg 125100

ttgtcccacg gaccttgctg aatctgctcg caaagatcat agatcttggt aaactctgca 125160

tctgtcaggt cttctgtgtg gacataatat ttaaccaatt cttccatcga cttattcata 125220

aataaacccc tctctgtggt taagagcaat agatgctcgt ggttctgcta tgcctgtagc 125280

atacatgtac ggatgacctt tcggagaatt cctcattccg aatgacccta attctaaccc 125340

atatttgtct tccacgtcaa accctatttc agtttttact atacgtgttt ccagactata 125400

ataggtccaa agaaaattac acatatcctt tgcatatgct ataggtccat gtttcggata 125460

aaactcaatc aattccagtt tcagaaagat gttaaaatga ctttcatcaa gaacatcttc 125520

tgtgcggtaa catggggtca atgccatata agggtgttct gctacaaact caaaaccatc 125580

tttctccatc tggaggaagg attgttctgc gctggcaaca tagaatctcc cattaggatg 125640

ccccaaaggg caatcctctt taccttctgg tagtgtgtat gcaagaatat ctgcatctac 125700

caggtaaggt acttgaatca tttgtgcata gcgtccgtaa tagtggatcg cattggcaat 125760

gacttcccca ttcatgatct tcctcctgtg cggaaagcac gtttggtaag gtctgacata 125820

gtgatcaaat cttgcaacgt catcccagga aactgtgtct ccagcacacc ttcaacagca 125880

cccacaatat cagacaattc aaccaattgc ataagtctgc aatcctgttc gacagcatca 125940

aaaagttcat aaacttcttc ctgaatcttt tcaatactgc cgtaaggttg tttctcaatg 126000

tctattaaat gataacccgc catattaata ctcctcacat cgaatctgct tgatcttacg 126060

agccaatgct gcaaccatct catcttcttc agtcatttta ggagatgtgt ctccccctaa 126120

ttgctgcatg gttaagatct catgcactgt ctcattccac tgcaaacatt taatacaacg 126180

tgtgtgagca acatacagaa ggttacgttc agattcttcc agtcctaccc actcgcgtgt 126240

cttcttatca tagttcgaag ggaagtcatt tgcaacgata acttggtccc attccaaacc 126300

tttcgatttg tgtgctgtgg tcagcgtcac ctttgctgta ggtgagttac ggtgcatacg 126360

gagtgtgtac gcaatgttgt tagctttacc tgaaacaaca atattcagta gacgtttagc 126420

gtctggatct gattctgcac aatccacaaa ctcctgccag tctgcatacg ggagaacagt 126480

ctcatgcttc actttatcaa tatctccgcg acgcaatgca ttcacactgt ccaccatagc 126540

aataaaatct ttggtgtcaa cgttaatatt gatatcttct ccgttcgcaa tacgatccat 126600

tgcatccata agcaacgtca ggtttttacg gaagatgatt gtgtacggtt tggagaagtc 126660

cactatatcc gagtggaagc tacctgtcac cgtgttatac cctttcccta ccaatggatg 126720

ggccagggga gttttatcac taaggatcgc attagcaata tctgccactt taggaccaaa 126780

acggaaacat gctgtcaatg gtaattctac accatattga gccgtctctt tcatcatgtt 126840

ctgactacct cggaactgat aaatactctg gaactcatca cctacaacca caatacgact 126900

ggccttttct gcgttacgca ggatagacag gaacgcaggg ttaacatcct ggaactcatc 126960

accaaagatg atatcataac ctaaatcttc gcccgatagt tcgaaaagtt tcacataggt 127020

gtcatgagtc atgatcgttt cagagttagg atcaatacgc tcacgccaca gttgttttgc 127080

atatttaacg acttctgcga tcagcttatt ctcattcaca ttcttacgtt tcaggtcaga 127140

aatgtgcatt ttagggaagt gtttcaaaga gatatgctca tcatctgaaa actcgtattt 127200

agccaaagtt tgtttaacca ttaaaccgat catagcacga gtgaccaaag tctttccgtt 127260

tttatcgata aggttttgaa tcttaaactt cttggcgatc tcactacctg ttcccgccac 127320

attcacatac ttcccttcag gacgtgagag tttgtgacgt aaattttgcg gaagcttctg 127380

atagcagatt gagtgcagag ttcgacactc tacattactc ggcatcttac gacgcgcttc 127440

ttctgccatg cttttgttga acgccaggta caggatgctc ttgtctgtgc catttgccag 127500

catgtatagg gtagatgttt tccccgaacc agattttgct tgaatagtta caaaacggtt 127560

ttccccgaaa gcacgctcaa tagcaaattg ctcttcggtt ggagtgaaac tcaaattatt 127620

agacactaaa tcctccttag taaaaactct tgacaatatt cattaaatga aaggcccgac 127680

tcgcatacgg ctctctcaag tttggtggga gatgggaacg tatataacgt tcctgtttct 127740

cccatgacca ttttgttttg acttttggta ttgtatcacc tgcattgtta ttttcaactc 127800

ttatttgcac tgtccagttg gaagggaagt gttcaccacc tttcttgaac ggtatgtagt 127860

gccctaaagt aaactctttc ttgttcttta gtctcccagc aagattaagg ttgacaatca 127920

tgttacggaa tacaaaatat tgataaagat tgtcaagagt atatacagcg tagtatgccc 127980

taaacgcgct atccctgctc atacctttga tatccatacg gcaccagttt aggtttgtag 128040

gatcataatc atgagcatca atccactcct ccctttcttc aaactctgcg ttaaccgcag 128100

attgtccagc agtggcaacg aataaggaat ctacgtcaaa gaacatacta cctcctgtta 128160

tttcacatcg tgcagtgcat tacctacttt caggacgatc acatcattaa tagcaccttc 128220

tccgttcaga tcgctgaaca caggggtgac aaaggtaatg atataatatg catcaacttg 128280

aatcacggca atcttcccat caagctttac atctcgtctg aatttgaagc catatagctt 128340

ctctgttaaa gggagatcac cctctttcaa caaagccaaa tcaaagatac gatacttttc 128400

tgacccgaca taaatgtagg taagcttgtc ttcttcatag tggtacagca tagcttgctt 128460

gtcttgcaag acgcttgcac caaatacatc caccgcaata atgttcttca tagattctcc 128520

tcccagttcc aatacccatc cacaaacggt ggattacgtc tatcgtattc ccttcgcgcc 128580

catgccacgg ctgcaacgag cacatctccg tgacatttct ggggcgagca aaagcagacc 128640

acatcttttc cttccaactc caacaaatct tcaagactta tcttaccaga acgaatctga 128700

tcccacaacc attctttata acgttgaatt gtagatcctc tcggctcatc atcttttaat 128760

ttgaaggggt tggcaaactt agacgctttc aacccgaagt ggggcatagc gcgtccaatg 128820

taaactgctc catctggaat aactttacct aaatggtaga agttcaagac tttcatatta 128880

ccccaaacac atacgtgcga tcaagctttg cgttaacttt tctgccgctt tataatcgta 128940

atcgacttcc tctaccccac cgtcttcatc acggaagact ttcccttcca ggaatccggc 129000

atcaatcagt ccaatataca gatcatactg ccaacaggag ttaccaaaag gtcgtttacc 129060

gctgaagcta tcctcttctt cccataacgt ggaaagaagt tggcggaaga actcacgcac 129120

agagacttta cctaaatctt gatgttcgaa catcacatct aaagcgtctg catcaccttt 129180

atctacaaac gggacgtaat gttcaccatc aatagtgact tgcaatacca tctttctcac 129240

ttttcctcct ttttgtattc aatgaacata tctgcatagt aagctttacg ttcttcaaat 129300

ttagcagggt ttatcacaat gaacaacact tttcggttat cttgtggttc tttgaccgta 129360

atgtagattt ctttggtgca ataaaaacct gttgctttat aagcgtcgcc ccaattatct 129420

actccacgat ctatacgatc aaacttacag ttatactttt tatcatccac ctggtagaca 129480

gcttcaccac cgtaccaagt gaagaattgg ctaccttcca ccccaatctt gaaaccgtca 129540

cgggcggctg tagcattggt ggcaatcatc aacatcagga cacaaagtag ctttttcatt 129600

tagaattcct cttcagtctt ctgtcggttt ttgaagaata ctacatcata tacatctccg 129660

taaccatcat ttacagagaa catgataaaa cgatcatctt ctgctgttgg tgcgcgatgt 129720

tcaagttcaa aactttcaat aaaaccgcgc gaatttacgt agtagatttc gtcctcacca 129780

gagaacatcc agtccctgtc tatcagatag aaagataaac tcacaggcga caactcatcc 129840

ttcacagaat cattgtacag catgtgggct gcaactgcca aacttaaagt cgtattgtac 129900

tgcattacac ctcctcgtca gactcatctg ctttagagaa tacaacatta aacgtttcac 129960

cgcaaccatt atttacgaaa tacatgataa agttttcatc ttcatctaca gggccacggt 130020

attccagttc gtagctatac agagaactat tgcttgtggc tccctgcgga taccagaaat 130080

agatctcatc gaaagattta acccacttca aacctgtacg atcctcaaac actaatgagg 130140

aaggattgtc agcatctttg acagcttctt cataatcctg gtgaaacttg actgctgttg 130200

ataatgtgct cattatgcct cctcgtaatg tttgcaaaca acgtgtctcc acgtcttctt 130260

gtaatgttct cgcttcgtca tagcttcttg ataagaggga atacgctgta acagaagtct 130320

ccattcgcag tctacgccca tacgtccgta caaagcataa cgaacattat ttttcttgtc 130380

ttgcatactc cctccgatca gttaattaaa ctttttccca agtgccaaat gcacattcag 130440

ggtaaatccc cacctgcatg ttaccattgt catctttgaa tgtaaagctg ttgttctctt 130500

tattcaaagt ggtggagtag gttttaccga tcgtcacaca acctgcactg tgtcccagtt 130560

gagtcagtga cagacatttc attttttgtt tcttcgtagc catttaaatc tccttttgtt 130620

tagtgagatt gcattctatc agaatacaac ctcgtgatca agacttattt gcagattaag 130680

attattctcg gattgggcta acagtcagat gcgctttcaa gattttccac acacgatctt 130740

gagagaaggt atcttgcgga gaagtgatcc ctgtatcaaa cataactgct aaaccgccat 130800

ctactttctg ccagatagga ggctcaccag aagcgtcagc accgccatag acaaagaaag 130860

tgccttctgg aatatcccgc aggaagtaaa gcttaggcgg cttctctgtg taagagatgg 130920

tattcaatgt tcctaatgta tttgccaggt tgctatctga cccactctct tggtagtcac 130980

ggattgcttg caagttgata atagagtgga accaatcttc agcagtttcc aggctacgtg 131040

gtgcagggta gtaatccagg aagttgctat caccccaacc gtaagttgta actgggccat 131100

gattacgtgg ataggcagaa tacaggttta aggtgccatc ctggttctgt gtcatgtatt 131160

gagcatcgtc acgaagcaca ctaagatcca taaccatcgc atagtctaac aatttcattc 131220

tttatctcct aagtttaaac agtttaagaa gtcacgaagg ataatcattt gttctgtaga 131280

caatgagagt acaccttcac cataaatatc accagcccat tcagcagtaa gatctaatac 131340

gccagtccca gggaaccaat ccacagtgat cctattacca ttcgagtcgg gcagtatacc 131400

attaattaac ataacacctc ctctatatta tgttcttgat cttacgatca tctaacgata 131460

agaacaagat ctaagtttct gttcttcaaa caatctaacg atagaaacta taattactac 131520

cctctaccct aacgggaggg taacacttat acctcgctcc cgctcggcac ttcgtgggta 131580

gggtagcata tctgaaaaca aaagtcaaca gttatttttt ggttaacaga taaatcagtt 131640

gttcacttgc ttctggtgca tagagtgcct tagtgtcaga gtcaaagaag tgagtgactc 131700

ctgtagcctt agatactgtg atagatagac gtgcaagctg gatcagttta tccatttcag 131760

caatctttgc acggaatact gtacgtgtag cgtcaaaagc tgcatgtggc atgtacttgc 131820

gatttagagc actgcgtttg aatctacgga agtctgcaat aatccggtta cgttctttat 131880

tcaagtgttg taccagtgca gtgtatacat gtggttcttt gttagccatt gttaatctcc 131940

tcttcataag ttggtagttc atgatatgcg atcacaggtg ccaagtcaaa ataaaaatga 132000

ccattgataa cagctaccgt gccattgcgt gttttgttaa cttcacagaa tcccttcttg 132060

ccgtttgcca tgattacaaa ccactctccg ataggaacat cctcggtgcc agatacttta 132120

atccatccac tcattttact tcccctttgt aatcagacac tacaccaggt cttacttttg 132180

ccttaagatg tttcaactct gccataggag ttgccacttg tatagggtta acttcaacct 132240

cgttgatagt atactcataa cccgcatact tcccgatgga gtgcaggtat ttgatagcat 132300

gttcaggtga atctgcttca acatacccca aatcttttgt agtgcggcct tcacagtcgc 132360

cttctgtagt tacgtgataa agtgtcataa aacctccttc ttgttggctg cttcaatcat 132420

agcgttaaga gtctccatac cgctttcaac tgcttgagca atctcttgga acacagactt 132480

cttaaagatg tataaagacc ctgcctgtaa gcgttgcaca caacataaga tcgcttggtg 132540

atccatcagg aagtcgccgt aggctgaatc aatcttacct gtaattgggt tctctcgtga 132600

acgaggatca tactctaaag taatgtgtgt cacgcctggc aaggtaacaa actggcagcg 132660

aatattaaca cgcggtgcga ttgacacgct aaacagattg ttggacattt aaacctcctc 132720

gtagtaaggc agagccttga tcaattgttc tgaaatctca ttctgcaccc atgtagtccc 132780

gttgtcaagc gaaacataac cttttttatc gacaaactta cgtagacaac cagtgaactt 132840

gtttttgtag atcatttctt ctttcctttc ttcagtttgt tatgaccttt acctttgtaa 132900

agcttgtgat tacgagcaga cggtttattt tggttatcga tacgggatac cggagtttgc 132960

cacattgtat ctccagtggc gaaagatgcc gccacaagaa gttccaagtg atggtagtcc 133020

agttcaggca tctttgtttc acgcatttcg cgttctacat tagacaatag ctcttcttcc 133080

tcaatcacag accaaccctc ttcttgccaa gcctcctctc tgaaccaagt ttccatgtta 133140

taaccgtgtt cgtcgagaat aacaccatcc cgcatctcaa accattcacc accacaaaaa 133200

tggcggtgct caacacgtct accttccttc attgcttctt tagcttcggt ccaagtcagt 133260

ttgatgtttt gcatttgttt tctcctctca tttggtatga gctaatcata acagaacaaa 133320

aaagcttgtc aatacttgat ctgaaatatt tttagtgtac aatagcccta cattattaag 133380

agaggtgaac aatgaacatt acattagcaa ctaagaacat ttcacaaact atcaacggtt 133440

ttggtgttat cgtggctttg cacgggaata atgagaagtg gttagcatct ttcgaagaag 133500

atgaaaagca caatgttatc ctgaacgctc ctatcaaggt gatgcacatc gttggtgaga 133560

gtaaggtact tacttcttct cgttcagagg aacaaattac aaatgaatta gtaaaactgc 133620

ttgacaacgt tcttcggatg tttgataatg cacccatcga cacaaaacgt gtcttaatgt 133680

gagaggagaa atacaatggc ttaccctgac tacatcgacg aacgtgctac tggcttcttc 133740

cgtaacttct tggttggtcg ttctcacggg gatggtggtt ccagttataa aacaagctgg 133800

gatagtcaat gtggcgctaa ggccaccaaa actttgaaaa tgccaggtcg tccattgtgc 133860

gcgtattgcg gaaatgaagc cttatccctt cagcctggta ttgagcatgg ggattatgat 133920

attaaaggtt acacttgtgt ctgcaaaggc gcaatggacg aggcggattg ggtgaaagaa 133980

aaggcagaga tggaggaacg tcacagacga gaagtttatg aaatggaaaa acgtatgcct 134040

gttccagatc ttcaagttaa gaaagatatt gttgcaaaaa tgatggaaaa agttcagaaa 134100

gcagagactg aacaagaact aaaccgtgcg atcgagaact tacgtaattt taaagaagaa 134160

aaagagttgc gcgagattat gtggtaaggt atactacttc catcaactaa gagaggagaa 134220

caaaatgtac tattcacaag acgaagtggc aaacatcaaa ttaacagttg gcgatcttat 134280

tgtatcccct aactatcact gggataacac ggacatggtt gaagtagtaa cccatgtcgg 134340

ggttgaaggc gttgttctct acactctggg tggaggtgtt gttggggaaa tggaacgtta 134400

tacgacaacg aagaaactgg tgagttctta tcgtatgttc ttacacaaag gttcggaaga 134460

gtatgcagcc ttcttattga aattggacgc agaacgagga gaaacaaaat gaaacttatc 134520

catgatgaat tacagtttga cgtagaagat ctgcaagatt tggacgatac gttctccgcg 134580

attatctgtg ctggattgta tgcgttcaag gcacgtattg tggaacgagg ggatatcctt 134640

atcccatcta agatttgggc taaatatgta tcagattacc gtaaactaac agaagagcag 134700

gagcgggaag ctacagtgga gtggttatcc attattgatc aaatgatcga aggttttaaa 134760

cctgaatcag ttatgaccaa aacagataaa atggaaggtc gttgcctgtt tgctgaatac 134820

tttcataact tgtggagcta actatgtctg aattttttac agtggttttc ttagggattt 134880

atctggttat taatgctgtt ggcttaatca tgtctctaat agagaatatg ggaattaagt 134940

gggcttgtac attcggtata gctcttatgc ttagtcctgt ttggatttac atctgcaagg 135000

cagttcaact aattagttaa ggaggaaata tgtttagtaa agaaatgaga gatgtgatcg 135060

ctactctggt agttgatcac ggtattgagg ttgagcgcca ggtttggaaa gatgaggcac 135120

ctttctttga cttagcatca cgagcgaaaa gtcacatgca tctcctggaa gacggtaatg 135180

agtttgttct ccgtatgcgt tatgatcgag aatttcgaat tcctcttgac actgaaccgg 135240

atggtgtagt attgtggttg gcagaacatt tcgcatacga tgcactacac ggacggggtt 135300

tcggtagtga acgttgggat gctgtttgtc gtaaacacca tattgaacca gactacggga 135360

gtatgtaatg ttaagcgtta agcctaagat gatcagtgaa gaccagaaag atttcattta 135420

caaatgtgga cgaatctgga gagagtatac caagggtaac gaggtcgtgg tggttactga 135480

acaccagatt gaaatctctg atcttttcgg tgattatttg caagtgatca aattaggcat 135540

tgacagaggt ttggttgaag tgctaacctt atctcaagtt gaggcacacc acttagataa 135600

aactattgtg gaattgtagg aggtaatatg ttagatctta ttatcaaagc aagtttgaca 135660

ggttgtttgc taagtgcgtt gtctgcacct atcataggcg aatttcattc tcaattaggg 135720

atgtttatcg ctttaagcgt ggctactctg ttcttggttg tagctgcatt atgtatgttg 135780

ttggaataag aggagatcac catgaaaaag attattttag ccgtagccct gctgtctgca 135840

agtttcgtag catctgctgc accgtttgtg gctgcacaat ccacaagtac cggagaagtt 135900

gcagtcaccg ctaaaggtca gttcgcttat actgatcctg atggcaagtt cttttcttgt 135960

acggatgtaa ccccgtctgt ggatgtggac aattctaatc gcccatacgt ggctatcggc 136020

gctgtgtgtg gcaacaactt catcatcatg aagcagtata aggatgatcg cggtggtagc 136080

ctcctgatcc gtcaagatgt tgaacgtaag aagaaagctg tggtgatgga agctatcatc 136140

actaaaggtg aagggatcta aaataagtat tgacaagacg tattagacaa gagataatac 136200

gtctattcca acagaagagg agaaagtaaa tgaaatacac agtttatggg gatgttgcaa 136260

ttgttaataa agacttcggt gtagaggttg ataaactccc cgctggcgtt tggactgtca 136320

attgtgatcc acatggtaac tattctctta gtcgtgctga taagttcacg attcctgatc 136380

gcctgtacgg ggaaaccaaa gaacgtgcag cccacgttat ccgtaccttc gctcgtcgta 136440

gtaaagaagg taaaaacaca ggtgttctgt tgtcgggtac aaaaggcagt ggcaagacaa 136500

tgctggcaaa actgatcagt aacactctgt tggaagcaga agacctggga atcccaacta 136560

ttctggtaac gcagccatac tctgatgcca acttcctgga gttcatgagt aagatctctg 136620

aacgcgcagt agtactgttc gatgagttcg acaaagtgta ttccaagaag gaagatcagg 136680

aagcactgct gactctgctg gatggcacag gtagcggtaa caaactgttc atgctgacaa 136740

agaacagcgg atacatctcg gaatacttcg tcaaccgtcc gagccgtatc ttctatagct 136800

tcaactacga caaactgtct attgaaacta tgctggacta cctggacaag aatctgaaga 136860

atcgcaaaca tgttgaaagc ttccaacgtt tgtgggatgt tagcactgaa ctgtctttcg 136920

atgttattca agggatcgta gaagagttaa acttctatcc gaaaatgacg ttcaagacct 136980

gtctggagat gatgggtatc tctctgggta acgatgcacg ctggacgatt agctctattg 137040

ttgtcaacgg taaagctatc aagcctaact gggtcaacaa ctggcatgag ttcactccta 137100

ccaagttctt gtctggcgca tgtgaactga acatctacct ggaagcggtg aaagacgaag 137160

aactggagcc tctcgaagat agcagttacg tcatcctgga tgatgatggt gatccacgca 137220

tcgacctgaa agctactgcc gaaggtctga agtgtgacct gattgaaggt ggtaagattg 137280

tagtagtgag cgataaacct ggagatacgt tccgtgtggt tctggaacca accactacac 137340

gatctgcaac actgagcagt gtgttctgat aaagatgccc cgcaaggggc atttctcaca 137400

tatggaggaa attatgttag ttgatgttca aatcatgcgt aacgaaaaaa tggatttctt 137460

tgaagcagta gagaaacatc tgattcgatg ggactatgaa tttgttcctg ctggatgtgg 137520

accagtagag catttagcgg aagacgatga tgtatacatg atcattcgtc aagtgtacca 137580

gacagaccag tgtgaagaaa ctcctgaact gattctgttt gaatgtgcag accttgcaaa 137640

atactttgat tacgttgttg ctaacagcga cgagttggct tatactgaat ccagatacag 137700

taaacgtcta cttatcttag attacatgaa agagaacaaa cacaaactca tcgacgcaga 137760

cgcggtttat ggctactggt ggatggacta acaaaggaga aattatggca cgtttagcag 137820

atcatgaaat ctttaaacct attctgaacc actatgatga aattaaagca gtgttggatt 137880

gttatagaga ggatgaagaa cgtttggaag tcaatcttaa gatagttctg atgaaagctt 137940

ttcaagacgg gcaagctgaa gtcttaagag gtatgaaaaa tgcgaataac tgatgaatat 138000

gtattcttct tttcacataa agatgtgttt agcaattggt acattgcgcc atttacagag 138060

acggaaccag ggtacagtga cactttctgc tgtgtagagc aatacatgat gtggcgtaag 138120

gctttgctgt tcaaggatca ggtaatcgca gcagctatcc tttatcactc aatgaagcgt 138180

gacgacgaga aggcacaggc atactacaag cgcatgggtc gagcggtgtc tggttttaat 138240

aatgatgtgt gggaagagca ccgagagcgt atcgtaatgc gaggcttatg cttgaagtat 138300

acacagaacc ctgatcttta ctctgatttg cgactctacc aattcaaaac gttcgttgag 138360

gcgagtcctt atgataaggt ttacggaatt ggaatgggta tgtatgaacc aggggtgcta 138420

aaccctgcca actggaaagg gcagaatctt ttaggacaat accacaacaa tctgattaaa 138480

atcttgttcc cagccaaaat accataaagg cgatattcgt gatattttga aaaaattcgt 138540

ttttaaaaag aatacgtcaa ctttgtgcca cagaaataag gctcctacaa caccgtagag 138600

agccttttct ctttagctac accaacactc atcttttaga gtttacctcg ttagacgagc 138660

attcaggtag gttgtaggct gcgtagcatc ttcaggaaag attatctcaa atcttacaag 138720

caaactatct caaaaatccc tacaattatt taaacttttt atgagtttac aaaagtgaga 138780

atccaaaacc aaattctaaa gtttacgtac attatctcaa atgggtagac cagatcgcaa 138840

agcaaaaaac cgagaaaaat tttttaaaaa gtcgcgcgca ccaggtattt aaagtttttt 138900

tgcttttaca aaagtgaaag ttgaaaagca aattctaaag tttgcgtgaa aattaccgaa 138960

atgagaatta ttctcactta aaatcgaaat acgaatgaga atgagaatga ttctcattca 139020

actactaaat gcgaatgagt cttatttgca aatgagaatt tttctcattt ccagaatgac 139080

gggcaggtcc ggtgattgtc tcgcgctccc gtgtcccctc gccatgtaat aagaataaca 139140

gatccaacca ggaaagcaag cgattatttt tatctttaca tttctttaca aatatatagc 139200

gcccagcctc ccggcgatcg accttccaac cagtgcccct ggtatgttag ccccgccttt 139260

acgccgtggg gtggcgcttc ctcttctgta tgtaaaacag tataagcccg atccctgggc 139320

ttgtcaagat tattttttat cttcttgcca ggataggaca cctaaccaaa aatacatatc 139380

ccccgcgccc ctggcggtat attgctttcc ccgccgccac tgcaagccca taaataacca 139440

tcagcgaacg aatcaaaatg atctatctct tccactttat gatcgcccag ggtgagaact 139500

gtttcaacct ttaagcccgt tgcgcttgct acttggtgga ccgtgccgcc ttgccagccc 139560

aacgagtgca taaggtgtaa tgtagtagtt gtcatggtgt cgcccttctt tgttgtgcgc 139620

cctggttggc gcggtagaaa gatgatagcc ctttccttta tacgtagcaa gcaaaaagaa 139680

aataaaaaag attgaaaagt ggttgacgtt ccgcacctgg tagcgtagta ttcatcttgt 139740

cgaggggggc gaggcacaac gacaataaat aaaaggaggg cgcaatgcag gacgggcaaa 139800

ggtgaggatc tccataatcc accgtcaacc aacaaaaaac atcattcatc acctggagtt 139860

aagatcatgg caacattgca catagtagca aaagcaggac aaatcagcgt agtggtaaac 139920

ggtactaatg aaatgagtgt acccgatgac gaatatatca cggtgctttc ctttcttgat 139980

tcactggtag aaatgggcgt tataacagaa gatcagcacg ctattgctaa tcgccgtgtc 140040

ttacatatcg tagggcttga ataaaaagta ttgacaacag gcaacaacct gataagatgt 140100

atcacgtaga gcggcggaat ggtccaccgc tcacaactaa gagagattat catcatggca 140160

tcatatagcg caacaatgat cgtaactggc accactggta aacctatgag cggtggcgga 140220

tctttcgcta atatcaatat tgacgggcgt ttatccctgg acgcagcgat cgccattgca 140280

cgggaaacat ttaagaaaga atgtaatttc aacaaacaag attatctcgg cttcgctatt 140340

gaaaaaaccg cgcgttttgt caattataaa agcccgaaaa tggttgatac tacattaaca 140400

gcaagacaag taaacttttt gctgtaaggg ggctaacgtg cattttctta aggtatgcgg 140460

gaagatcata gcctttcgta acgaggaatt ttataacctt gctcgttatg ttatgatcta 140520

ccctggcaag gtatataaaa acagtccgac aagttacact atttacaatg tgctatcagg 140580

taaaaccacg gagatcataa aagcaggggt gagagattat cgcacatctt tctacccttg 140640

ccgtgattcc gccagtttat tttttgtgtg tgaaatgatt gttaaaaggg gtgtataatg 140700

gggcgcgaat atgtgatcca gtcctgggta aaacatgacg ctaatgatcc gcgtattata 140760

ccggatgaca tgatcgatca tgctcctggc tatagtggcg agcaaatagg taacagatcg 140820

gcgcgtagtc atgggaatac caaaacacgc agcgatcgcc ctggttggct ggcgaatggt 140880

aacgctgaca ttgcaagcgc cgcagatgat agcgacggcg cgaaagataa tccgctgtac 140940

aaggtagatc cggcttttcg tgtcctggat gaatacccca gcaagatagc ggcgcttgat 141000

gccgcgctcg aaagtaagcg catcacctgg acggcgtaca gtactgcaaa aagcgtatta 141060

gatggtaagc tgatccaggc tgaaaggaaa ttggccaaaa ttgcagccat agcccaggag 141120

gaagaccaag aagaggaaga cgatcacgcg gttgagtgcc tcgcctttga tcacttcctc 141180

ccagtttttg aggcatcgca ggaagagaag aaagaagccg aaaaaaatta tctttatttt 141240

gatgatttag gttgacaggt aaaacgtgat cacttatagt taactcatcg ggcggcaacg 141300

gtgccgctca caactgaaag gatagaaaaa tgtttactac ttccctattt tttgcaatca 141360

attttattcc tggtttccgt gctgcacttg aaatcattct ttggacactg taaggaaaac 141420

atgatgatca agaaagaaaa tagtttgaca gttggaacgc ttaacgctat tatcttggtt 141480

gttccatttt ggatcaccgt ggcagttatc acaacaatca ttttcaagta aggggcacac 141540

tatgagtaag gttatccgtc acgaaaaact aagccctaca ttatctatta gtgaatgcaa 141600

tgatggttat tggctatacg atgaaacgcg cggcatgaat ttagctatgc aagcgaaaac 141660

cgaacaaacg gcatatgtgc aagccctgga atactaccag cgacgactac aggaagtcga 141720

aaacgactat aaaagcctga aatcaaaggt tgatcacttt atcagccagg tagcagatga 141780

cgacgatgat cactactgcg aacgatgcgg atcatatagt taaaaatatc tttgacaacg 141840

tttaaaatta gtgtaagatg tatcacatag ggcggcggaa tggtccaccg ctcaaaacag 141900

aagagagatt atcatgaaac acttatcatc tatcaccaac cataatgaaa ttttcgttaa 141960

tgaaaaaggt ttcgcctgga ttcagttaaa agagatgaaa aataaccgtc gtgtaacggt 142020

tgttcgtaaa atcaagagta aaccttttag caatttttca gtggacaccg tgcggatcaa 142080

gaatgaagaa tacttgatcg atagtcaaat cacttggagc ttaacggcat cagccgccgc 142140

gccatatttc gcaaactaag aaaaaaacgt ttgacactgt tttaaattct gatatagttt 142200

atcacgtagg gcggcaatgg tgccgccaac aataaaaggt taacatcatg gctatcattg 142260

caaaaactga gattataggt ttagattcga tcctcctggc ttatgaggaa actttaaaaa 142320

tgtatgtagt gacctatggt gattatgtgc gtgaatacga atacattgat cttgctttcg 142380

aatgttacat tcgccaggtc aataaagcct ataacgatta tcgttaaaag ttttagtctt 142440

atactattga caatgagtga tagtataagc ataataactt ttatcgggaa tacttcccac 142500

taaccaaaaa gagagagtaa catcatggct attaatatta atgtttccgc ttatgctacc 142560

ctggctaata ctgctaaaat tgaactatcc agcattgagg ctgtgcacat tagcgcccgt 142620

cgctggtttc aacagtccta cggcaataca tatttttctt taagtgtaga cgttgagatc 142680

tgcggtaaat tgattgaagt ggttaacgtg ccgtttcaat atggttatgg tgatcatttc 142740

gataccgtcg ccctggaaga atttagtaag gttatcaacc tggaaggcaa ggaattttca 142800

cgcggcgcgt atctttcccg cttttgccgt gacaatggga tcactgtata cagtcatgca 142860

agcgatgtta aacgtaaaaa agatttataa aaagcattga cagcacgagt aaaaagagta 142920

aactgtatta catagggcgc gggaatagtt cccgccccac tgaaaaaaga gattaacatc 142980

atggcaaaat caaaaatgtt cttaggtcac gctaacaaaa ctcattggaa tgtaagcctt 143040

tggattaata acgatgaatc attgtatcgg ttggccctgg attttattgc cgctaatact 143100

aatcgtaatg atgcggcccg tcatatgatg ctatttttag aacaaactgg acaagataaa 143160

actccagacg gtttcaagta tagcactacc gcgatccgcg ccgctatggt tggaatgtaa 143220

aggagacata aaaatgaaac atcgttcgac ctatctcaca aaacgtttta gcttagatca 143280

taaattgggc tattacggaa gaaatagaaa ataatttgac aaagttttaa attagtgtaa 143340

gatgtatcac ataggggcga caagcgcccc gcctaacaga agagagaaaa atcatggcta 143400

tcaatattgt tcgcgtatcc ggcaccgttg gcacttccca ggttattgca gttgtaacaa 143460

gcaaaaagat taatgatgta tgtgagatct tgaataagtg ggctattgct aattttggag 143520

aatatgcgag cgttgagtac atcgaagctt atcaggtgcc aaccatcagc ctgaccgatc 143580

tcaacgttga tcgcctgtta tgcctggatg aattaatctg aaaaagattg ttgacaccgg 143640

aaagaaaaag agtaaactgt attacgtagg gcggcaatgg tgccgctcac aacaaagaga 143700

gattatcccg atggctaata tccgcacact gttaaaaaac gcaagtcaag ttttattttc 143760

cgatcacgat catttcaata aagacgttac aggcgtaaaa aatatcaaag atgtaaaaag 143820

tgttatgggt atctattgca atatgaaacg tgttggaacg ttcggcgata aagtattata 143880

tcaggcgggg gattacaatt ttactttgac aattatttca taaaaccgct tgcaatcttg 143940

ataaaataaa gtaaactgta ttacatcaag gcggcggaat ggtccaccgc ctaacaaaga 144000

gaagaggaaa acatcatgat tacttttact acccgtgaag ccgctcgcaa cgttgcccgt 144060

aaagttaacg ccgctaatct gggcgcaagt gtcaaggcac ctgtaaaaga tgaatccggc 144120

gcttggatct tccctggtct gcgtcacgct gataatctgg gcaccttaag ccgtaaaaaa 144180

taatttgact tttcgttata acgccctata atgggcgtta tagagaagag aaaccaatta 144240

ccacaaagag agaaagaaaa tggcaatccg taaaattaaa gttatttgca ttgaatcaac 144300

tggtggcgct tttgtagttg gtcagaaata taatgcggtt ttaatgggca tggattggaa 144360

ggttaaaggt ctggatggtt acgaatacgg cactactcac ggattgcgtg gtcgaatcgg 144420

atacgtgagc ttcgacaaca aagaaaaatt tgttttcgat attgttgtga gtaaatagca 144480

tgaattgcta aagactaatc tgcgaattgc cgtattatca actaatcgaa atgaaacaca 144540

taaagagaaa aacattatga tcactttcgc tattgtcgcc gttgtcgctg gtcaagttta 144600

cgtaaacgat acattcacca cagaagacaa agcagcggga gacattaccg ccgcttataa 144660

cgagtgcaag gaagtaggaa ggatgttagc gccttgcttg cttatgagtg aacgccagga 144720

cgggatcacg gtgtttacta accttgaaac agggtatcaa atgatagtag aaaaatgatt 144780

gacaagtttt aaaaatccgg taagatgtaa caagtagggc ggcaatggtg ccgccaacaa 144840

cagaagagag acaccatgaa agcacaaaat gcaattaagc tttatattct ggcctcaatc 144900

gattatagtg agtatggcat cacagaagat aaagagaaga tcgcggaatt gatgcgccgc 144960

tttaagtctg agtactggta tcccgataat caaaaacgtt acgatcataa cattatcaga 145020

gcaatagcat catggttaca aggtttaccg tcagatatta atatcgcttt cgagtattgg 145080

gaagtagatc ggttgttaac atcctggggg tacattaacg agcgtagtag cgaaaacaag 145140

atccagaaag aaagggaaaa ctattggtta tatattgcag gtgtgatcct tacactttcc 145200

cacgaataaa aagtttttaa aagtagttga caggcatcaa gcaataaagt aaatttaaac 145260

acgtagggcg gcaatggtgc cgctcacaac taaaaagagg aaacatcatg aatacatcta 145320

atcgcgccga aaaaatccaa tcttccaaaa cacgcgtagc gtggaatgag gaagtgatcc 145380

gcaagggcga caagcgcaac aaaactaagc gcgaccgcac cgagaaacgt gcgtggatgg 145440

acctggaaga ctaaaacaaa tcaagggcgc aaatagcgcc ctttcttata tctaacgaga 145500

ggataacagc acatgacctt aaacgagatt aaaacagcct acaacgcagc gaacaaagtg 145560

gcagcacgtt accgcgacga atacggtttt attgcgcaag gcgtgatcct gggcgtttct 145620

aacgatggtt tgatttacat tgatgcttat cataaccaag ttaaaccgga aaacgttatc 145680

agtataaaat atctttaaaa gtggttgaca ccataaccgg acctgttaaa ctgtattaca 145740

tcaggcgggg aatagttccc caccatacaa aaaggtaaat attatgatca ccgcaaaaca 145800

agaaaaaatc attatcgacg ttgcagccgt tgccgctatt gctattattg gatcttatta 145860

tgatacccgt ttcccatcgg ctatgcatga gatcgccgcc gtggttagtg tcgctgtact 145920

gttttacgcg ggtaaacgtt atttgtcacc aattgcaaat aaacttgctt tttggaagaa 145980

ataaggtaaa gtgtattacg tagggcggca atggtgccgc gcccgtttga ggatcaaatc 146040

atggttaaag ctaacacggt gctactgttt tccctggtta atgatgccgc ttacttgtcc 146100

aacatgacgc aagatgatag cacattaacg gcaacgttta ccgatggact taaccgagtg 146160

atcatcgttt cactgaaaac agggagtaaa ggcttttact cgttggctta tcattgggta 146220

acagaaagcg acgagacaag ccaggactat agcgccccat tccctgaatc aatggcgcgg 146280

gagatcatcg ccgccgttgt aaatgatggt ctgtttactg cataaaaata atttagaaaa 146340

aggcttgcaa tcattttggg atagtgtata tttaagtcat agggcggcgg gaatagttct 146400

caaagcaagc gagaggataa cacaatgaac aaatacacag tagaatacac ggtacaaaat 146460

ccggcagtat ccgaagggcg taagactacg atcgccgttg gtgttatcgc tttcgttatc 146520

actatcttat caatcatctt ataaggtgca caccatgaaa acagtaaacg ttatcaggtc 146580

atcaaacatc attaaagaga tcgcccttgc cactggcatt gagatcgagg aagtaatcgc 146640

gctcaatctt acagaaaacg atctgcgcaa ctatgaagcg gaaagcgtga aagcaagcgg 146700

cgcggtcctg gcatataatc cggctaacaa gtaaggggct aacatgaatc cgaaagatat 146760

gcaagcatgg aaagggtacg acgatctaag tgccttgcgc aataagtcaa gccgtaacct 146820

tagacggttg gaagcaaaga aaaagaaaaa tattaagggg gtgcaccatg cataacaata 146880

tcattgcaga tcgtgccgct actattcgcc caggagatcg ggcgcgttta caggttggcg 146940

tttatccgtt cccgttttat gatttctgta aggtaacggc tatcgaaaac aatccgtttg 147000

atcctgacac cgtgatcatc tggtacgaag gaaggggaaa cagatatgag gatatcatag 147060

ggcgtgatga atccgtaaac atcctacgct actaatcgcc agctaacagc ctaaaaggtg 147120

taaggcaatg caatccccta cctgatttaa ttagtgcgcc ctggcttatg ctggggcgtt 147180

ttacgttgta gaaaataatt ttaaaaaata tgcttgacgc ctggaacgtt ggcgagtata 147240

gtttatctca tagggcggcg gaacggtcca ccgcaaaaca gaagagaaaa catcatgact 147300

acttacaaga aaacgaaaga actggtaaaa ggcgacctgg ttaaaaacat ccttaacggg 147360

aagtattatc cggtagtatc agttgaacgt tggaatagcg ggtacatcgt taaacttgat 147420

agccgtgacg atgggtgtga gttttacgct gaatacagcg atcgccacac cgtgcaataa 147480

aaatagttga cagatatccc gaaagggatt atctttatca gacaggggcg ataaacgccc 147540

cataacaaga aaggtaacaa catgaaaaag attgattctt cccgcgtatt gtgtgaaatc 147600

gatgttatca tcggtaacgg caccggacac cataaactga tcaagggtaa ggcttatcgc 147660

ctggcaacgc tcaaagcaga tatgcgccta acagcaagtc agatcaaaaa gttttttcgc 147720

ccgttgttga caacagcaaa gtaagcgcgt agtattcatc ttgtagggcg gggcaataag 147780

gcaacgccca ttaatgaggg tagcatcatg ttagagaaaa ttttcacggt agtaatcatt 147840

ggtctgactt tatcggttgg agtggttgca actatggcta acatgatcct atcttcccac 147900

ggtattggat aaggtgaatc aaatgatcac gaataagaca ggtaaaacag aatttttacg 147960

cggcttgcag atcggcaagc ctgagatctg gcattgcagc aagtccgcac ataacgaaat 148020

gaaagacttt accgctacgt tacaacgtgt taaaatatct attacacaga aaaaagtctt 148080

gttagtagta gaaaacgaga tcccgcaaac tattattatt gtcgagcgta cagcatgaaa 148140

acagaaatca ttattgaaat catcaacggt tataaagaag cctttatttt tgctgaatca 148200

gccagggatc acgaaggtaa ttttattgat aacttagacg attatgattt ttcagatcaa 148260

gcccatcagc ggatcattca tgacgtagcg gcgttttgtg atgccaatga tgccgctatc 148320

ttggaagcga tgagcgacgg cgcaacagcc aaccagatcg ggaatgattt acattttacc 148380

cgtaatggtc acggcgtagg cttttgggat cgccctgaaa tatacaccac taacacagcc 148440

aaccgcttaa ccaatgcagc taagtttatg ccgaacgtgt cggcttacat tggtgaagat 148500

aatttgattt atattgaata aaaagtattg acacagatcg ccagtgtgat aatatttacc 148560

acgtagggcg gcaatggtgc cgctcaccga aaaaggtaaa catcatgaaa actatcagct 148620

acctggaatc gaataacatg ttttttgctc ccgttgatga aatcatcgaa gccttaaaca 148680

acgaagaggg gatcaatggt ttatcttaca ctgaactttt acaaatggtt gcagcaaatg 148740

aagagcttgc ttactatgtt gaaaattttg atgaagctgt aatctttaca aaataatcct 148800

tgatctaatc tcttagataa cttatattta atgcgtaggg cggcaatggt gccgccgata 148860

atgaggaaat taaaatggaa actatcgcta ttgttaccgt agaaaaactg gttgttgggg 148920

atgtattcgc gttaatggtg cctaacagca gcaaactggg cgcttatgaa gtgatggatc 148980

ataaaaatga atcatttggc gatggttgca caatggcggt aaaatcctta aaggatggaa 149040

tcactcgcca ggctaactgg cacaaaggga atcaagtcgg tttaatatcc gctaatttct 149100

tgccagaaaa gcaaacatta gaccaactat caggcggtaa gacgttagcc gaactactgg 149160

aaacagccat ctaatcaaca aaggggcggc aacgccccac atcagaaagg aaaagattat 149220

gagtattgca acagtattaa acgccgttaa aaccgctgaa tcatccggct tacatgttga 149280

gcttatagat caaccaggtg gcgatctcct tgtccaggtt tacccgccga aagtagggga 149340

tgattattat tggtgctatt ctgatacgtt cgaacaagta gacacgaaag cgatcacagc 149400

ttttatttta aaagccgttg aagaaaataa ctttcatcat aacatcaatc aataaaccat 149460

aagcggggcg gcaacgcccc cattaaacgc aataggaaga ggatagacac atgaaaaaga 149520

tgatcgcagt agttgccctg gttgttggta ttaacgcgcc tgtaatggcg aaagagctgg 149580

caaaacacgt tgacctaacg gatatccatg ccgtcaatcg gatcggggtt acttatcgtt 149640

tatgccttgc cgctaaaact tttaaggacg gcacaacaga gtattacgga ttcgagggta 149700

tccgctgcgg tgagcttgca cgcgacctta aggaagatcc agaccttgaa accatgcaga 149760

tgaccgtaaa cggtaaagct tacgtaaacc gccaacaatg agaaaggtaa tcatgacaat 149820

ggggatcata gtagcaacgc tggcgctaat ccccttcctg attagatatg tgatagtaga 149880

tctggtttta gttgttagtg acctgctatc ttccatcggc acactgtttt aagggtacat 149940

catgaacgtg gaacaagtta gaaaggtatt agcagcgtta cgctatcaca accatagcaa 150000

ggtgaagaat gatcctgatt atgttgattc aagcctggaa gaagacacct tgcaagctat 150060

aagccatatg caagaattgc ttaaacgtat gacagagaag aacagatctg attatatagg 150120

tgacgagatc taatctttac aaaacagggc ttgtttttgt cctgtttttt gttatgctta 150180

ttgaacgggc ggcactggca acgggggagg taatcaccgg acgaccccgc tatatattga 150240

tagctaacac ctatcaaaca acaaaacatc aaagagataa actatcgaat ctttgcaatt 150300

ctacctattg acaccggatc acccttatat tattatttat cacgtagggc gggaatgttt 150360

cccgtctaat ataggaataa catcatgacc gaacaaatta aaagcactaa accgaacgtt 150420

aacggcaatg tttcacactt tatcggtgat aatgagagag gtacacccta cgccgccgta 150480

accgttcaac ctgacggtaa ttattggttt accgtttacg gatcttattc ctttggacgt 150540

ccggcacatg ataagatctt gcaagcggtt aagcctaccg gaaagaatat cacggcagaa 150600

aaattttata aaaaataact tgctttatag ctcctgttaa cttatagtta ttgcgtaggg 150660

cgagcaatac agcaagccca caaagaggaa aagaagatga gcaaatcagc aattaaagca 150720

gttgaaaaag cgcaagaagt gatcgcaacg tccaccactg gcgcaaaggt taatctcgtt 150780

ggttatctga cagatgaaac tatccgtaaa aattggcgca acgctgatct actggcttgc 150840

ttattgatta atagcgtcgc taatgctttc cagtgtgaaa cagccgaaag cctggaggaa 150900

aacttgttcg acaatatgga tcaagccatt gaaacctata ttcaatcaaa cttggatagc 150960

tggaacgcta agacatggcc cgacagttta cgcgatacaa ctgatcaact gatccgcctt 151020

gccctgatgg ttgaacgcga taacgtttac aacagcacaa agtaagattg tacaaggggc 151080

gcactaagcg ccctttaacg taaagaaatg taaataatgc gctatcacct attgacaggg 151140

gatcggcgtt atattatatt taactcactg gcagcaaaca acgaaacgaa acaaaggaca 151200

acaacatgaa cattaaatca aataatccgg cttactcctt cgataacgct ttttgccacg 151260

gtagcgccgc gaaaggcttc gaaattgatg gtaagctggt tattgtgaac gttgacgggg 151320

aagaggttgc agtatatccg gtaaacgatg ttaaaagccg tttacaatgc cgcatggatg 151380

ttaccgcgta tcagcttgat ccgatccgtg tagttgccct ggtcctggaa gaagagatca 151440

aagcaatgca tcatagtaac aagtttattg gtgacgcgca ggacaatatg aaagtatgcc 151500

gcagtaaagt taatgaagcc ttgaaagaat gcggttttaa ggatctgatg attaaacgtg 151560

atgaaaaatt ccgctttcgt aatgacctgg cgaaccggat ctactcaata tatgaagcgt 151620

tataattgac aacaaaacaa gggcgcacaa agcgcccttt ctttttgccc gaacaatgtt 151680

gatagcttcc gcctatgaaa aaccgacaca gaacaaaact gaatagatcg cggtatcgag 151740

ctaatcaacc attaaacgaa taaccataca ccccagggca acaggctgtt agagagctat 151800

ttagagcggt taaaatcgat caacaaccaa caacaaacga ctgaaaaaga ctgttttctt 151860

cctttccagt gcaatgataa ccattatcat ctacccttgc gtatctatgc aatataagtg 151920

cataactata atgttgataa tagttcgcaa caactaacca accaacaacc taaaacaata 151980

agcaaactaa tcataatcaa aggaataata gaaagatgat tgataacgac aaacaatcaa 152040

accatgatga atactaagca agataacgat aacacgacaa aacaaccaac taactaacga 152100

taacaaatga aaccattaaa ataataacta ttgccgaaat agtgaagact gtaataattg 152160

caaaatgaat cattccaagc ctcatcatta gcacgctacg ccggacacgt agcgagctaa 152220

taataagcca actacacccc cacaatcata actatcggtg atagttattt cttgtgatta 152280

tgtagcttgc aattattgca agcgtattac gcttacacta attacgctgg cttattatta 152340

gcattgtatg taagaatagt taagcgtaat ataatactca atgttattgt tgcatgatgt 152400

aatcattagc agggttaagg attatgcggg gggcaaggcg agcgaatagc gataccttgc 152460

acactgtaca gatatacaga aaataatctt ttgcggggga gacatgacac gaccgaaggg 152520

cggggatgtt g 152531

Claims (16)

1. 一种用于预防或治疗由致病性大肠杆菌引起的感染或疾病的组合物，包括：

具有裂解致病性大肠杆菌的能力的肌尾噬菌体科噬菌体，和

药学上可接受的载体。

2. 根据权利要求1所述的组合物，其中肌尾噬菌体科噬菌体包括如SEQ ID NO:1所述的序列。

3. 根据权利要求1所述的组合物，其中肌尾噬菌体科噬菌体的浓度为1×10¹ pfu/ml至1×10³⁰ pfu/ml或1×10¹ pfu/g至1×10³⁰pfu/g。

4. 根据权利要求3所述的组合物，其中肌尾噬菌体科噬菌体的浓度为1×10⁴ pfu/ml至1×10¹⁵pfu/ml或1×10⁴pfu/g至1×10¹⁵pfu/g。

5.根据权利要求1所述的组合物，其中药学上可接受的载体是乳糖、葡萄糖、蔗糖、山梨醇、甘露醇、淀粉、阿拉伯胶、磷酸钙、海藻酸盐、明胶、硅酸钙、微晶纤维素、聚乙烯吡咯烷酮、纤维素、水、糖浆、甲基纤维素、甲基羟基苯甲酸酯、丙基羟基苯甲酸酯、滑石粉、硬脂酸镁或矿物油。

6.根据权利要求1所述的组合物，还包括：

选自由润滑剂、润湿剂、甜味剂、香味剂、乳化剂、悬浮剂和防腐剂组成的组的一个或多个。

7.根据权利要求1所述的组合物，其中致病性大肠杆菌是肠出血性大肠杆菌、产肠毒素大肠杆菌、肠侵袭性大肠杆菌、肠致病性大肠杆菌、肠聚集性大肠杆菌或致癌性大肠杆菌。

8.根据权利要求1所述的组合物，其中感染或疾病是食物中毒、胃肠炎、腹泻、尿路感染、新生儿脑膜炎、溶血性尿毒症综合征、腹膜炎、乳腺炎、败血症、革兰氏阴性肺炎、志贺氏菌病、痢疾或癌症。

9.根据权利要求1所述的组合物，其中组合物是溶液、悬浮液、油乳剂、水溶性介质、提取物、粉末、颗粒、片剂或胶囊。

10. 根据权利要求1所述的组合物，其中肌尾噬菌体科噬菌体具有20-70分钟的潜伏期和80-200 PFU/感染细胞的裂解量。

11. 根据权利要求1所述的组合物，其中潜伏期为35-40分钟，裂解量为110-120 PFU/感染细胞。

12.根据权利要求1所述的组合物，进一步包括：

具有裂解致病性大肠杆菌或非大肠杆菌细菌菌种的能力的第二噬菌体。

13. 一种用于预防或治疗由致病性大肠杆菌引起的感染或疾病的方法，包括：

向受试者给药肌尾噬菌体科噬菌体；以及

用肌尾噬菌体科噬菌体裂解致病性大肠杆菌。

14. 根据权利要求13所述的方法，其中肌尾噬菌体科噬菌体包括如SEQ ID NO:1所述的序列。

15. 根据权利要求13所述的方法，其中肌尾噬菌体科噬菌体的浓度为1×10¹ pfu/ml至1×10³⁰ pfu/ml或1×10¹ pfu/g至1×10³⁰ pfu/g。

16. 根据权利要求15所述的方法，其中肌尾噬菌体科噬菌体的浓度为1×10⁴ pfu/ml至1×10¹⁵ pfu/ml或1×10⁴ pfu/g至1×10¹⁵ pfu/g。

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