CN115444773A - Anti-inflammatory repairing composition containing glycol salicylate, preparation method and application - Google Patents
Anti-inflammatory repairing composition containing glycol salicylate, preparation method and application Download PDFInfo
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- CN115444773A CN115444773A CN202211022382.9A CN202211022382A CN115444773A CN 115444773 A CN115444773 A CN 115444773A CN 202211022382 A CN202211022382 A CN 202211022382A CN 115444773 A CN115444773 A CN 115444773A
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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Abstract
The invention discloses an anti-inflammatory repairing composition containing glycol salicylate, a preparation method and an application thereof, wherein the anti-inflammatory repairing composition containing glycol salicylate comprises the following components: a base oil phase comprising olive oil and jojoba oil; active phase including Rosa canina fruit oil, ethylhexyl ferulate, grapeseed oil, glycol salicylate, and Chrysanthemum morifolium oil. The anti-inflammatory repair composition containing glycol salicylate is verified by a plurality of tests such as chick embryo chorioallantoic membrane tests and the like, can solve the symptoms of various skin inflammations, adopts active substances such as Rosa canina fruit oil, ethylhexyl ferulate, grape seed oil, glycol salicylate and chamomile oil, and then mixes the base oil mixed by olive fruit oil and jojoba seed oil with the active substances to obtain the anti-inflammatory repair composition, and the anti-inflammatory repair composition can effectively solve the problems of various specific skin inflammations.
Description
Technical Field
The invention relates to the field of daily chemicals, in particular to an anti-inflammatory repairing composition containing glycol salicylate, a preparation method and application thereof.
Background
An allergic reaction is an extraordinary or pathological reaction of the human body to an antigen. During the allergic reaction, the allergen (antigen) plays a direct role, being the external cause of the allergic reaction; the body has low immunity, and the oxidative damage of a large number of free radicals to cells is the internal cause of anaphylactic reaction. Skin allergy is a common skin problem, and with the increasing use of cosmetics and other chemicals, environmental problems, accelerated pace of modern society life, increased stress and other problems becoming more severe, more and more people have sensitive skin.
The chamomile oil contains chamomile azulene, alpha-bisabolol and sesquiterpene lactone, can bring an anti-inflammatory effect, the jojoba seed oil can be mixed with sebum, regulates the humidity of the skin, reduces the loss of moisture of the epidermis, has a balanced fatty acid composition and contains a small amount of palmitate, provides good antioxidant stability, and has the content of linoleic acid and linolenic acid of the rosa canina fruit oil of about 80 percent, and the glycol salicylate is mainly used for effectively responding to various specific skin inflammations.
There is a need for a mild, non-irritating anti-inflammatory remedial composition and products for its use that provide remedial benefits.
Disclosure of Invention
The invention mainly aims to provide an anti-inflammatory repairing composition containing glycol salicylate, and preparation and application thereof, and aims to solve allergic symptoms and repair skin.
In order to achieve the above object, the present invention provides an anti-inflammatory repairing composition containing glycol salicylate, comprising:
a base oil phase comprising olive fruit oil and jojoba seed oil;
an active phase comprising Rosa canina fruit oil, ethylhexyl ferulate, grapeseed oil, glycol salicylate, and Chrysanthemum bailii oil.
Further, the anti-inflammatory repairing composition containing glycol salicylate comprises the following components in percentage by weight: 50-95 parts of olive fruit oil, 10-50 parts of jojoba seed oil, 1-5 parts of rosa canina fruit oil, 0.1-2.5 parts of ethylhexyl ferulate, 20-70 parts of grape seed oil, 1-5 parts of glycol salicylate and 0.1-9 parts of chamomile oil.
Further, the active phase comprises Rosa canina fruit oil, dimethyl methoxy chromanol, grapeseed oil, ethylene glycol salicylate, and Chrysanthemum Ieucanthemum oil.
Further, the active phase comprises Rosa canina fruit oil, BHT, grapeseed oil, glycol salicylate, and Chrysanthemum morifolium oil.
Further, the active phase comprises Rosa canina fruit oil, tocopherol (vitamin E), grapeseed oil, ethylene glycol salicylate, and chamomile oil.
Further, the anti-inflammatory repairing composition containing glycol salicylate can be compounded with any one or more of retinol, retinal, phytosterol, ceramide and HPR.
Furthermore, the anti-inflammatory repairing composition containing the glycol salicylate can be compounded with any one or more of ferulic acid, ethyl ferulate, 4-butyl resorcinol, phenethyl resorcinol and glycyrrhetinate.
The invention also provides a preparation method of the anti-inflammatory repairing composition containing glycol salicylate, which comprises the following steps:
s1: pre-dispersing ethylhexyl ferulate with Rosa canina fruit oil to obtain a first phase;
s2: mixing the chrysanthemum morifolium oil and glycol salicylate to obtain a second phase;
s3, mixing the olive fruit oil with jojoba seed oil to obtain a third substance phase;
s4: mixing grape seed oil into the first phase;
and S5, mixing the first phase, the second phase and the third phase to obtain the anti-inflammatory repairing composition containing glycol salicylate.
The invention also provides an anti-inflammatory repair cream containing glycol salicylate, the anti-inflammatory repair cream containing glycol salicylate comprises:
emulsifiers including cetearyl alcohol, cetearyl glucoside, water, PEG-100 stearate, glycerol;
a co-emulsifier comprising cetearyl alcohol;
an emollient comprising polydimethylsiloxane, isohexadecane;
a cooling agent comprising menthol, menthol lactate;
a solvent comprising water;
a thickening agent comprising carbomer, xanthan gum, gum arabic;
a preservative comprising propylparaben;
a chelating agent comprising disodium EDTA;
a humectant comprising glycerin, 1, 2-hexanediol;
an anti-inflammatory rejuvenating composition as claimed in any one of the preceding claims.
Further, the anti-inflammatory repair cream can be compounded with moisturizers and skin grease, wherein the moisturizers comprise sodium hyaluronate and panthenol; the skin caring lipid comprises isononyl isononanoate, shea butter and squalane.
Of course, the person skilled in the art will be able to apply the invention to other cosmetic products according to the actual requirements.
The anti-inflammatory repairing composition is prepared by premixing ethylhexyl ferulate with rosa canina fruit oil to obtain a first component capable of preventing oil rancidity, mixing grape seed oil, pre-dispersed chrysanthemum morifolium oil and glycol salicylate with the first component to obtain an active phase, and mixing a base oil phase formed by mixing olive fruit oil and jojoba seed oil with the active phase.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all directional indications (such as up, down, left, right, front, back \8230;) in the embodiments of the present invention are only used to explain the relative positional relationship between the components in a particular pose, the motion, etc., and if the particular pose is changed, the directional indication is correspondingly changed.
In addition, the descriptions relating to "first", "second", etc. in the present invention are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
The chemical formula is as follows:
ethylene glycol salicylate:
ethylhexyl ferulate:
example 1
An anti-inflammatory repairing composition containing glycol salicylate, which comprises the following components in percentage by weight:
58 parts of olive fruit oil, 35.5 parts of jojoba seed oil, 2 parts of ethylhexyl ferulate, 3 parts of rosa canina fruit oil, 0.5 part of chrysanthemum oil of white-flowered blume and 1 part of glycol salicylate.
The preparation method comprises the following steps:
s1: weighing 2 parts of ethylhexyl ferulate and 3 parts of rosa canina fruit oil, and pre-dispersing 2 parts of ethylhexyl ferulate with the rosa canina fruit oil to obtain a first phase;
s2: weighing 0.5 part of chrysanthemum morifolium oil and 1 part of glycol salicylate, and mixing to obtain a second phase;
s3, weighing 58 parts of olive fruit oil and 35.5 parts of jojoba seed oil, and mixing to obtain a third phase;
s4, mixing the first phase, the second phase and the third phase to obtain the example 1.
Example 2
An anti-inflammatory repairing composition containing glycol salicylate, which comprises the following components in percentage by weight:
58 parts of olive fruit oil, 31.5 parts of jojoba seed oil, 2 parts of ethylhexyl ferulate, 4 parts of rosa canina fruit oil, 3 parts of grape seed oil, 0.5 part of chamomile oil and 1 part of glycol salicylate.
The preparation method comprises the following steps:
s1: weighing 2 parts of ethylhexyl ferulate and 4 parts of rosa canina fruit oil, and pre-dispersing 2 parts of ethylhexyl ferulate with the rosa canina fruit oil to obtain a first phase;
s2: weighing 0.5 part of chrysanthemum oil and 1 part of glycol salicylate, and mixing to obtain a second phase;
s3, weighing 58 parts of olive fruit oil and 31.5 parts of jojoba seed oil, and mixing to obtain a third phase;
s4, weighing 3 parts of grape seed oil, and mixing 3 parts of grape seed oil into the first physical phase;
s5, the first phase, the second phase and the third phase are mixed to obtain example 2.
Example 3
An anti-inflammatory repairing composition containing glycol salicylate, which comprises the following components in percentage by weight:
57 parts of olive fruit oil, 35.5 parts of jojoba seed oil, 2 parts of ethylhexyl ferulate, 3 parts of rosa canina fruit oil, 0.5 part of chrysanthemum oil of white-flower spring yellow and 2 parts of glycol salicylate.
The preparation method comprises the following steps:
s1: weighing 2 parts of ethylhexyl ferulate and 3 parts of rosa canina fruit oil, and pre-dispersing 2 parts of ethylhexyl ferulate by using the rosa canina fruit oil to obtain a first phase;
s2: weighing 0.5 part of chrysanthemum oil and 2 parts of glycol salicylate, and mixing to obtain a second phase;
s3, weighing 57 parts of olive fruit oil and 35.5 parts of jojoba seed oil, and mixing to obtain a third phase;
s4, the first phase is mixed with the second phase and the third phase to obtain example 3.
Example 4
An anti-inflammatory repairing composition containing glycol salicylate, which comprises the following components in percentage by weight:
57 parts of olive fruit oil, 31.5 parts of jojoba seed oil, 2 parts of ethylhexyl ferulate, 4 parts of rosa canina fruit oil, 3 parts of grape seed oil, 0.5 part of chamomile oil and 2 parts of glycol salicylate.
The preparation method comprises the following steps:
s1: weighing 2 parts of ethylhexyl ferulate and 4 parts of rosa canina fruit oil, and pre-dispersing 2 parts of ethylhexyl ferulate with the rosa canina fruit oil to obtain a first phase;
s2: weighing 0.5 part of chrysanthemum morifolium oil and 2 parts of glycol salicylate, and mixing to obtain a second phase;
s3, weighing 57 parts of olive fruit oil and 31.5 parts of jojoba seed oil, and mixing to obtain a third phase;
s4, weighing 3 parts of grape seed oil, and mixing 3 parts of grape seed oil into the first physical phase;
s5, the first phase is mixed with the second phase and the third phase to obtain example 4.
Example 5
An anti-inflammatory repairing composition containing glycol salicylate, which comprises the following components in percentage by weight:
57 parts of olive fruit oil, 31.5 parts of jojoba seed oil, 2 parts of ethylhexyl ferulate, 3 parts of rosa canina fruit oil, 4 parts of grape seed oil, 0.5 part of chamomile oil and 2 parts of glycol salicylate.
The preparation method comprises the following steps:
s1: weighing 2 parts of ethylhexyl ferulate and 3 parts of rosa canina fruit oil, and pre-dispersing 2 parts of ethylhexyl ferulate with the rosa canina fruit oil to obtain a first phase;
s2: weighing 0.5 part of chrysanthemum oil and 2 parts of glycol salicylate, and mixing to obtain a second phase;
s3, weighing 57 parts of olive fruit oil and 31.5 parts of jojoba seed oil, and mixing to obtain a third phase;
s4, weighing 4 parts of grape seed oil, and mixing the 4 parts of grape seed oil into the first physical phase;
s5, the first phase, the second phase and the third phase are mixed to obtain example 5.
Comparative example 1
An anti-inflammatory repairing composition containing glycol salicylate, which comprises the following components in percentage by weight:
57 parts of olive fruit oil, 30.5 parts of jojoba seed oil, 2 parts of ethylhexyl ferulate, 3 parts of rosa canina fruit oil, 0.5 part of chrysanthemum oil of white-flower spring yellow and 7 parts of glycol salicylate.
The preparation method comprises the following steps:
s1: weighing 2 parts of ethylhexyl ferulate and 3 parts of rosa canina fruit oil, and pre-dispersing 2 parts of ethylhexyl ferulate with the rosa canina fruit oil to obtain a first phase;
s2: weighing 0.5 part of chrysanthemum morifolium oil and 7 parts of glycol salicylate, and mixing to obtain a second phase;
s3, weighing 57 parts of olive fruit oil and 30.5 parts of jojoba seed oil, and mixing to obtain a third phase;
s4, mixing the first phase, the second phase and the third phase to obtain a comparative example 1.
Comparative example 2
An anti-inflammatory repairing composition containing glycol salicylate, which comprises the following components in percentage by weight:
57 parts of olive fruit oil, 35.5 parts of jojoba seed oil, 3 parts of ethylhexyl ferulate, 3 parts of rosa canina fruit oil, 0.5 part of chrysanthemum oil of white-flower spring yellow and 1 part of glycol salicylate.
The preparation method comprises the following steps:
s1: weighing 3 parts of ethylhexyl ferulate and 3 parts of rosa canina fruit oil, and pre-dispersing the 3 parts of ethylhexyl ferulate by using the rosa canina fruit oil to obtain a first phase;
s2: weighing 0.5 part of chrysanthemum morifolium oil and 1 part of glycol salicylate, and mixing to obtain a second phase;
s3, weighing 57 parts of olive fruit oil and 35.5 parts of jojoba seed oil, and mixing to obtain a third phase;
s4, mixing the first phase, the second phase and the third phase to obtain a comparative example 2.
The following are comparisons of the effectiveness tests performed using examples 1-5 and comparative examples 1-2, and the specific tests are as follows:
chick embryo chorioallantoic membrane irritation test:
chick embryo chorioallantoic membrane (CAM) test is performed to determine whether the product is irritating at different concentration ratios. The egg shell of the air chamber part of a nine-day-old chick embryo is stripped, a white egg membrane is exposed, the egg membrane is moistened by 0.9% sodium chloride (NaCl) solution, the intima is removed by forceps, the vascular membrane is guaranteed not to be damaged, and a preliminary experiment and a formal experiment are carried out after the damaged blood vessel is confirmed not to be damaged.
The experimental steps are as follows:
s1, grouping tests, wherein each group contains at least 6 chick embryos, and a negative control, a positive control and a blank control are required to be set in each test;
s2, preparing CAM, namely performing egg lighting inspection on 9-day-old chick embryos, peeling off eggshell parts of air chambers, dripping physiological saline on the surfaces of eggshell membranes to fully wet the eggshell membranes, and then removing the eggshell membranes;
s3, placing the test ring on the CAM, and sucking 200 mu L of test substance to drip into the test ring;
s4, immediately observing the change degree of each toxic effect after acting for 3min, and giving a stimulation score (ES).
And (4) observing results:
bleeding: blood flows from the blood vessels or capillaries of the CAM. No bleeding, mild bleeding, moderate bleeding, and severe bleeding were scored 0, 1,2, and 3, respectively.
Blood coagulation: it refers to the condition of protein denaturation inside and outside blood vessels, such as thrombus, closed swelling, blood coagulation spots inside and outside blood vessels, opacification and turbidity outside blood vessels, etc. The blood coagulation was judged to be 0, 1,2 and 3 points for non-coagulation, mild coagulation, moderate coagulation and moderate coagulation, respectively.
Vascular lysis: refers to ablation of blood vessels on the CAM membrane. The scores of 0, 1,2 and 3 are respectively judged according to avascular ablation, mild vascular ablation, moderate vascular ablation and severe vascular ablation.
Results calculation and evaluation: the average score of ES was calculated according to the following formula, and ES = (sum of degrees of bleeding, coagulation, and vascular lysis observed in 6 chick embryos)/3 was judged for stimulation classification according to Table 1
| Endpoint scoring | Irritation classification |
| ES≤12 | No/light irritation |
| 12<ES<16 | Moderate irritation |
| ES≥16 | Severe irritation property |
TABLE 1 evaluation of ES results
TABLE 2 chick embryo irritation test results
The experimental conclusion is that: the product has no irritation under the condition of optimal concentration ratio.
(II) testing the antioxidant capacity of the vegetable oil by a DPPH free radical method:
DPPH radical scavenging ability
The experimental steps are as follows:
s1, setting a positive control substance, and dissolving and diluting the positive control substance into 0.08mg/mL, 0.04mg/mL, 0.02mg/mL and 0.01mg/mL series of concentration gradients by using 95% ethanol to verify a test system;
s2, treating a test object, diluting a water-soluble test object into a multi-concentration sample by using water, and diluting an oil-soluble test object into a multi-concentration sample by using 95% ethanol;
s3, setting a sample tube (T) and a sample background (T) by using a 10ml test tube 0 ) DPPH tube (C) and solvent background (C) 0 ) 3 parallel tubes need to be set for each sample tube (T) with each tested concentration of each sample, and three parallel tubes need to be set for the DPPH tube (C);
s4, in the sample tube (T) and the sample background (T) 0 ) Adding 1ml of sample solution with the same concentration into each sample solution;
s5, in all test tubes (T, T) 0 、C、C 0 ) Supplementing solvent, supplementing 3ml and mixing;
s6, in sample tube (T) and DPPH tube(C) Adding 1ml of DPPH ethanol solution, and obtaining the background sample (T) 0 ) And solvent background (C) 0 ) Replacing with 95% ethanol, shaking gently, and standing at room temperature for 5 min;
s7, transferring each reaction solution into a 1cm cuvette, and measuring the light absorption value at 517 nm.
DPPH clearance calculation formula: clearance (%) = (1- (T-T) 0 )/(C-C 0 ))×100％
In the formula:
t is the light absorption value of the sample tube, namely the light absorption value of the solution after the sample reacts with DPPH;
T 0 -sample background absorbance values;
c is the average value of 3 times of light absorption values of the DPPH tube, namely the light absorption value of the DPPH solution when no sample is added;
C 0 -background absorbance of solvent.
TABLE 3DPPH radical scavenging Capacity
The experimental conclusion is that: within the allowable adding range, the irritation of oxidative stress to the skin can be reduced by using the sample of 0.2mg/ml, so that the stronger the capacity of removing free radicals, the stronger the capacity of repairing the skin barrier.
(iii) capsaicin stimulates keratinocytes (soothing test):
the experimental steps are as follows:
s1, cell inoculation, according to 1.8E 5 (ii) inoculation Density of cells/well keratinocyte into six-well plate, and setting to 37 ℃, 5% CO 2 The incubator of (2) is incubated overnight;
s2, preparing a solution, wherein the concentration of the prepared positive control solution is 15.6 mu g/ml, and the concentration of the prepared sample is 0.8mg/ml;
s3, administration, namely performing grouped administration when the cell plating rate in the six-hole plate to be detected reaches 40-50%, wherein each group is provided with 3 multiple holes, and the administration amount of each hole is 2ml;
s4, placing the six-well plate at 37 ℃ after completion of the administration, 5% CO 2 The incubator is incubated for 24 hours;
s5, detecting capsaicin receptor (TRPV 1), namely fixing with 4% paraformaldehyde for 30min, and then carrying out immunofluorescence detection and analysis of the capsaicin receptor (TRPV 1).
TABLE 4 results of the relaxation test
And (4) experimental conclusion:
compared with the BC group, the TRPV1 protein content of the NC group is obviously increased, which indicates that the stimulation condition of the test is effective.
Compared with the NC group, the TRPV1 protein content of the PC group is obviously reduced, which indicates that the positive control detection is effective.
Compared with the NC group, when the concentration of the sample is 0.80mg/mL, the TRPV1 protein content is obviously reduced, and the protein expression quantity is reduced by 28.3%.
The experimental result shows that the anti-inflammatory repair composition containing glycol salicylate has anti-inflammatory repair efficacy, the irritation is obviously reduced compared with a comparative example in the prior art, and the anti-allergic effect of the product can be improved by adding the white flower spring yellow chrysanthemum oil.
The anti-inflammatory repair composition containing glycol salicylate is proved to have remarkable curative effect, no toxic or side effect, good stability and convenient use by patients through chick embryo chorioallantoic membrane test and DPPH free radical method determination vegetable oil oxidation resistance test and relaxation test. The irritation is improved when the proportion of the product is higher than the optimal proportion, so that repeated tests prove that the product has no irritation and free radical scavenging capacity in the optimal concentration.
With reference to all the embodiments, the anti-inflammatory repair composition containing glycol salicylate is verified by a chick embryo chorioallantoic membrane test, a DPPH free radical method determination, a soothing test and the like, and can be proved to have the function of aiming at the symptoms and mechanisms of skin inflammation.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the present specification and directly/indirectly applied to other related technical fields within the spirit of the present invention are included in the scope of the present invention.
Claims (10)
1. An anti-inflammatory remedial composition comprising glycol salicylate, comprising the following components: a base oil phase comprising olive fruit oil and jojoba seed oil;
the active phase comprises one or more of Rosa canina fruit oil, ethylhexyl ferulate, grapeseed oil, glycol salicylate, and Chrysanthemum bailii oil.
2. The anti-inflammatory remedial composition comprising glycol salicylate of claim 1 wherein the anti-inflammatory remedial composition comprising glycol salicylate comprises, in weight percent: 50-95 parts of olive fruit oil, 10-50 parts of jojoba seed oil, 1-5 parts of Rosa canina fruit oil, 0.1-2.5 parts of ethylhexyl ferulate, 20-70 parts of grape seed oil, 1-5 parts of glycol salicylate and 0.1-9 parts of chamomile oil.
3. The anti-inflammatory rejuvenating composition containing glycol salicylate of claim 1 wherein said active phase comprises Rosa canina fruit oil, dimethylmethoxychromanol, grapeseed oil, glycol salicylate, and Chrysanthemum bailii oil.
4. The anti-inflammatory remedial composition comprising glycol salicylate of claim 1 wherein the active phase comprises Rosa canina fruit oil, BHT, grapeseed oil, glycol salicylate, and Chrysanthemum morifolium oil.
5. The anti-inflammatory rejuvenating composition containing glycol salicylate of claim 1 wherein said active phase comprises Rosa canina fruit oil, tocopherol (vitamin E), grapeseed oil, glycol salicylate, and Chrysanthemum bailii oil.
6. The anti-inflammatory remedial composition comprising glycol salicylate of claim 1, wherein the anti-inflammatory remedial composition comprising glycol salicylate is capable of being formulated with any one or more of retinol, retinal, phytosterols, ceramides, HPR.
7. The anti-inflammatory remedial composition comprising glycol salicylate of claim 1 wherein the anti-inflammatory remedial composition comprising glycol salicylate is capable of formulating any one or more of ferulic acid, ethyl ferulate, 4-butyl resorcinol, phenylethyl resorcinol, and glycyrrhetinates.
8. A method of preparing an anti-inflammatory remedial composition comprising glycol salicylate, the method of preparing the anti-inflammatory remedial composition comprising glycol salicylate comprising the steps of:
pre-dispersing ethylhexyl ferulate with Rosa canina fruit oil to obtain a first phase;
mixing the chrysanthemum morifolium oil and glycol salicylate to obtain a second phase;
mixing olive fruit oil with jojoba seed oil to obtain a third phase;
mixing grape seed oil into the first phase;
mixing the first phase with the second phase and the third phase to obtain the anti-inflammatory repairing composition containing glycol salicylate.
9. An anti-inflammatory cream comprising glycol salicylate, wherein the anti-inflammatory cream comprises:
emulsifiers including cetearyl alcohol, cetearyl glucoside, water, PEG-100 stearate, glycerin;
a co-emulsifier comprising cetearyl alcohol;
an emollient comprising polydimethylsiloxane, isohexadecane;
a cooling agent comprising menthol, menthol lactate;
a solvent comprising water;
thickening agents including carbomers, xanthan gum, acacia gum;
a preservative comprising propylparaben;
a chelating agent comprising disodium EDTA;
a humectant comprising glycerin, 1, 2-hexanediol;
an anti-inflammatory remedial composition according to any of claims 1 to 7.
10. The anti-inflammatory repair cream of claim 9, wherein the anti-inflammatory repair cream is capable of formulating moisturizers, skin creams, said moisturizers comprising sodium hyaluronate, panthenol; the skin caring lipid comprises isononyl isononanoate, shea butter and squalane.
Priority Applications (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116115540A (en) * | 2023-03-20 | 2023-05-16 | 广东雅丽洁精细化工有限公司 | Skin anti-aging composition, application and daily chemical product |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004096169A1 (en) * | 2003-04-29 | 2004-11-11 | Provincia Italiana Della Congregazione Dei Figli Dell'immacolata Concezione - Istituto Dermopatico Dell'immacolata | Dermatological formulation |
| RU2668537C1 (en) * | 2017-04-19 | 2018-10-01 | Ирина Александровна Брынцева | Cosmetic product |
| CN108815039A (en) * | 2018-08-04 | 2018-11-16 | 彭红兵 | A kind of maintenance nourishing oil |
| CN109966183A (en) * | 2019-04-30 | 2019-07-05 | 广州栋方生物科技股份有限公司 | A kind of moisturizing remediation composition and its preparation method and application |
| CN110302103A (en) * | 2019-08-15 | 2019-10-08 | 武汉洛园生物科技有限公司 | A kind of the combination oil and preparation method of maintenance skin barrier |
| CN114533578A (en) * | 2022-03-10 | 2022-05-27 | 陕西畅想制药有限公司 | Cosmetic composition for removing pustular pox and preparation process thereof |
-
2022
- 2022-08-23 CN CN202211022382.9A patent/CN115444773B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004096169A1 (en) * | 2003-04-29 | 2004-11-11 | Provincia Italiana Della Congregazione Dei Figli Dell'immacolata Concezione - Istituto Dermopatico Dell'immacolata | Dermatological formulation |
| RU2668537C1 (en) * | 2017-04-19 | 2018-10-01 | Ирина Александровна Брынцева | Cosmetic product |
| CN108815039A (en) * | 2018-08-04 | 2018-11-16 | 彭红兵 | A kind of maintenance nourishing oil |
| CN109966183A (en) * | 2019-04-30 | 2019-07-05 | 广州栋方生物科技股份有限公司 | A kind of moisturizing remediation composition and its preparation method and application |
| CN110302103A (en) * | 2019-08-15 | 2019-10-08 | 武汉洛园生物科技有限公司 | A kind of the combination oil and preparation method of maintenance skin barrier |
| CN114533578A (en) * | 2022-03-10 | 2022-05-27 | 陕西畅想制药有限公司 | Cosmetic composition for removing pustular pox and preparation process thereof |
Non-Patent Citations (1)
| Title |
|---|
| 丛林;廖勇;杨蓉娅;: "敏感性皮肤治疗进展", 中国美容医学, no. 01 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116115540A (en) * | 2023-03-20 | 2023-05-16 | 广东雅丽洁精细化工有限公司 | Skin anti-aging composition, application and daily chemical product |
| CN116115540B (en) * | 2023-03-20 | 2023-10-24 | 广东雅丽洁精细化工有限公司 | Skin anti-aging composition, application and daily chemical product |
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