CN115380052A - 多肽的生产和纯化方法 - Google Patents

多肽的生产和纯化方法 Download PDF

Info

Publication number
CN115380052A
CN115380052A CN202080077127.7A CN202080077127A CN115380052A CN 115380052 A CN115380052 A CN 115380052A CN 202080077127 A CN202080077127 A CN 202080077127A CN 115380052 A CN115380052 A CN 115380052A
Authority
CN
China
Prior art keywords
fusion polypeptide
self
polypeptide
peptide
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202080077127.7A
Other languages
English (en)
Inventor
林章凛
景艳芸
杨晓锋
赵镭
佩吉·保利·艾米索·邓格
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South China University of Technology SCUT
Original Assignee
South China University of Technology SCUT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South China University of Technology SCUT filed Critical South China University of Technology SCUT
Publication of CN115380052A publication Critical patent/CN115380052A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/555Interferons [IFN]
    • C07K14/56IFN-alpha
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormone [GH], i.e. somatotropin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/06Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/90Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185Escherichia
    • C12R2001/19Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)

Abstract

本发明提供了包含目的多肽部分和自聚集肽部分的融合多肽,以及通过表达该融合多肽来生产和纯化目的多肽的方法。

Description

PCT国内申请,说明书已公开。

Claims (40)

  1. PCT国内申请,权利要求书已公开。
CN202080077127.7A 2019-10-31 2020-10-30 多肽的生产和纯化方法 Pending CN115380052A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201911053169.2A CN112745393A (zh) 2019-10-31 2019-10-31 多肽的生产和纯化方法
CN2019110531692 2019-10-31
PCT/CN2020/125054 WO2021083301A1 (zh) 2019-10-31 2020-10-30 多肽的生产和纯化方法

Publications (1)

Publication Number Publication Date
CN115380052A true CN115380052A (zh) 2022-11-22

Family

ID=75645030

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201911053169.2A Pending CN112745393A (zh) 2019-10-31 2019-10-31 多肽的生产和纯化方法
CN202080077127.7A Pending CN115380052A (zh) 2019-10-31 2020-10-30 多肽的生产和纯化方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN201911053169.2A Pending CN112745393A (zh) 2019-10-31 2019-10-31 多肽的生产和纯化方法

Country Status (3)

Country Link
US (1) US20220372074A1 (zh)
CN (2) CN112745393A (zh)
WO (1) WO2021083301A1 (zh)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113549144A (zh) * 2021-06-30 2021-10-26 华南理工大学 一种特立帕肽hPTH(1-34)的生产和纯化方法
CN114133435B (zh) * 2021-07-21 2024-06-18 浙江大学 一种类弹性蛋白多肽及应用
CN114350695A (zh) * 2021-12-20 2022-04-15 华南理工大学 一种含二硫键多肽人脑利钠肽hBNP的生产与纯化方法及应用
CN114891817A (zh) * 2022-04-15 2022-08-12 华南理工大学 一种多聚肽及其制备方法与应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102226172B (zh) * 2011-05-09 2013-06-05 清华大学 基于自聚集短肽诱导的酶聚集体的蛋白质纯化方法
CN104755502B (zh) * 2012-10-12 2018-05-18 清华大学 多肽的产生和纯化方法
CN107629129B (zh) * 2016-07-19 2023-08-04 清华大学 生产和纯化多肽的方法
CN108570439A (zh) * 2017-03-13 2018-09-25 浙江京新药业股份有限公司 氧化还原酶的融合蛋白、基因工程菌及其制备方法和用途
CN109627290B (zh) * 2018-12-12 2022-03-29 华南理工大学 α螺旋自组装短肽及其在蛋白质纯化中的应用

Also Published As

Publication number Publication date
CN112745393A (zh) 2021-05-04
WO2021083301A1 (zh) 2021-05-06
US20220372074A1 (en) 2022-11-24

Similar Documents

Publication Publication Date Title
CN115380052A (zh) 多肽的生产和纯化方法
US9200306B2 (en) Methods for production and purification of polypeptides
WO2020259403A1 (zh) 重组串联融合蛋白制备目标多肽的方法
CN107698684B (zh) 包含突变的免疫球蛋白Fc部分的GLP-1融合蛋白
EP2992021A2 (en) Novel cloning, expression & purification method for the preparation of ranibizumab
CN113549144A (zh) 一种特立帕肽hPTH(1-34)的生产和纯化方法
WO2022241831A1 (zh) 多肽的制备方法
KR101026526B1 (ko) 대장균에서 외래단백질을 분비 생산하는 방법
JP4088584B2 (ja) 融合タンパク質から目的タンパク質を分離する方法。
CN107629129B (zh) 生产和纯化多肽的方法
CN111363048B (zh) 一种具有穿膜活性的可溶性重组苦荞金属硫蛋白FtMT及其制备方法
US20110092424A1 (en) Production of glucagon like peptide 2 and analogs
Yang et al. High-level production of a candidacidal peptide lactoferrampin in Escherichia coli by fusion expression
WO1986003779A1 (en) Polypeptide secretion-causing vector, microorganisms transformed by said vector, and process for preparing polypeptide using said microorganisms
KR20220058631A (ko) 재조합 치료 펩타이드의 발현을 위한 n-말단 연장부 서열
US20200024321A1 (en) Expression and large-scale production of peptides
JP3251592B2 (ja) Il―16活性を有するプロセスされたポリペプチド、それらの製法、及びそれらの使用
KR20150009953A (ko) 1→3 해독 프레임 이동의 감소 방법
KR100535265B1 (ko) 융합 단백질로부터 목적 단백질을 분리하는 방법
CN117801123B (zh) 沃索利肽可溶性中间体、中间体制备方法及沃索利肽的制备方法
CN114350695A (zh) 一种含二硫键多肽人脑利钠肽hBNP的生产与纯化方法及应用
Wang et al. Overexpression and purification of recombinant atrial natriuretic peptide using hybrid fusion protein REF-ANP in Escherichia coli
US20230041904A1 (en) Method for enhancing water solubility of target protein by whep domain fusion
WO2009005201A1 (en) A method for producing stromal cell-derived factor-1a as an active soluble protein form
WO2000015664A1 (en) Modified human growth hormone

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination