CN115279902A - 用于调控基因表达的系统 - Google Patents

用于调控基因表达的系统 Download PDF

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Publication number
CN115279902A
CN115279902A CN202080072368.2A CN202080072368A CN115279902A CN 115279902 A CN115279902 A CN 115279902A CN 202080072368 A CN202080072368 A CN 202080072368A CN 115279902 A CN115279902 A CN 115279902A
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China
Prior art keywords
polya
aptamer
nucleic acid
seq
ligand
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Pending
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CN202080072368.2A
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English (en)
Chinese (zh)
Inventor
L·严
L·罗
J·D-Y·杰
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Baylor College of Medicine
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Baylor College of Medicine
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Publication date
Application filed by Baylor College of Medicine filed Critical Baylor College of Medicine
Publication of CN115279902A publication Critical patent/CN115279902A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080072368.2A 2019-08-30 2020-08-28 用于调控基因表达的系统 Pending CN115279902A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962894611P 2019-08-30 2019-08-30
US62/894,611 2019-08-30
US201962904635P 2019-09-23 2019-09-23
US62/904,635 2019-09-23
US202063043504P 2020-06-24 2020-06-24
US63/043,504 2020-06-24
PCT/US2020/048561 WO2021041924A2 (en) 2019-08-30 2020-08-28 System for regulating gene expression

Publications (1)

Publication Number Publication Date
CN115279902A true CN115279902A (zh) 2022-11-01

Family

ID=74683442

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080072368.2A Pending CN115279902A (zh) 2019-08-30 2020-08-28 用于调控基因表达的系统

Country Status (11)

Country Link
US (1) US20220290147A1 (https=)
EP (1) EP4022065A4 (https=)
JP (2) JP7737152B2 (https=)
KR (1) KR20220049619A (https=)
CN (1) CN115279902A (https=)
AU (1) AU2020335909A1 (https=)
BR (1) BR112022003512A2 (https=)
CA (1) CA3152513A1 (https=)
IL (1) IL290944A (https=)
MX (1) MX2022002390A (https=)
WO (1) WO2021041924A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102904879B1 (ko) 2015-11-12 2025-12-30 베이롤 칼리지 오브 메드신 폴리아데닐화의 압타머 매개된 조정을 통한 포유류 유전자 발현의 외인성 제어
AU2022327174A1 (en) 2021-08-11 2024-02-15 Sana Biotechnology, Inc. Inducible systems for altering gene expression in hypoimmunogenic cells

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003295600A1 (en) * 2002-11-14 2004-06-15 Dharmacon, Inc. Functional and hyperfunctional sirna
CA2559280A1 (en) 2004-03-15 2005-09-29 Biogen Idec Ma Inc. Methods and constructs for expressing polypeptide multimers in eukaryotic cells using alternative splicing
WO2005106035A2 (en) * 2004-04-09 2005-11-10 Cornell Research Foundation, Inc. Modular design and construction of nucleic acid molecules, aptamer-derived nucleic acid constructs, rna scaffolds, their expression, and methods of use
WO2005116204A1 (ja) * 2004-05-11 2005-12-08 Rnai Co., Ltd. Ｒｎａ干渉を生じさせるポリヌクレオチド、および、これを用いた遺伝子発現抑制方法
US7825229B2 (en) * 2005-03-25 2010-11-02 Rosetta Genomics Ltd. Lung cancer-related nucleic acids
US8178503B2 (en) * 2006-03-03 2012-05-15 International Business Machines Corporation Ribonucleic acid interference molecules and binding sites derived by analyzing intergenic and intronic regions of genomes
US20150284738A1 (en) * 2006-12-29 2015-10-08 Rodina Holding S.A. Artificial dna sequence with optimized leader function in 5' (5'-utr) for the improved expression of heterologous proteins in plants
CN103108952A (zh) * 2010-09-01 2013-05-15 日本电气株式会社 检测目标物质的方法以及用于所述方法中的适体组、传感器和装置
EP2622084A1 (en) * 2010-10-01 2013-08-07 R1 B3 Holding B.V. Regulation of translation of expressed genes
WO2013119371A2 (en) * 2012-02-10 2013-08-15 The Board Of Trustees Of The Leland Stanford Junior University Mini-intronic plasmid vectors
US20150141320A1 (en) * 2012-05-16 2015-05-21 Rana Therapeutics, Inc. Compositions and methods for modulating gene expression
US9567581B2 (en) * 2012-08-07 2017-02-14 The General Hospital Corporation Selective reactivation of genes on the inactive X chromosome
KR102699584B1 (ko) 2015-02-02 2024-08-28 메이라지티엑스 유케이 Ii 리미티드 대안적 스플라이싱의 압타머 매개 조절에 의한 유전자 발현 제어
EP3268481B1 (en) * 2015-03-11 2020-05-27 The United States of America, as represented by The Secretary, Department of Health and Human Services Rpgr vectors for treating x-linked retinitis pigmentosa
KR102904879B1 (ko) * 2015-11-12 2025-12-30 베이롤 칼리지 오브 메드신 폴리아데닐화의 압타머 매개된 조정을 통한 포유류 유전자 발현의 외인성 제어

Also Published As

Publication number Publication date
AU2020335909A1 (en) 2022-04-14
US20220290147A1 (en) 2022-09-15
IL290944A (en) 2022-04-01
WO2021041924A2 (en) 2021-03-04
MX2022002390A (es) 2022-05-13
JP2025118711A (ja) 2025-08-13
JP2022546408A (ja) 2022-11-04
BR112022003512A2 (pt) 2022-05-17
KR20220049619A (ko) 2022-04-21
EP4022065A2 (en) 2022-07-06
JP7737152B2 (ja) 2025-09-10
WO2021041924A3 (en) 2021-04-08
EP4022065A4 (en) 2024-02-14
CA3152513A1 (en) 2021-03-04

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