CN115252681B - Chinese herbal medicine composition for preventing carassius auratus gibelio overwintering syndrome and preparation and application thereof - Google Patents
Chinese herbal medicine composition for preventing carassius auratus gibelio overwintering syndrome and preparation and application thereof Download PDFInfo
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Abstract
The invention discloses a Chinese herbal medicine composition for preventing the winter syndrome of carassius auratus gibelio, and preparation and application thereof. Taking common andrographis herb, coptis root, radix isatidis, virgate wormwood herb, chrysanthemum and liquorice according to a certain proportion, firstly coarsely crushing, then crushing by an ultrafine grinder, sieving by a 100-mesh sieve, uniformly mixing, and preparing the powdery Chinese herbal medicine composition. The Chinese herbal medicine composition can improve the immunity of fish bodies, resist multiple bacterial infection and reduce the incidence rate of the winter-through syndrome of the carassius auratus gibelio.
Description
Technical Field
The invention relates to the field of aquaculture disease control, in particular to a Chinese herbal medicine composition for preventing the 'overwintering syndrome' of carassius auratus gibelio, and preparation and application thereof.
Background
Carassius auratus gibelio (Carassius auratus gibelio) is an improved carp species, which is becoming increasingly popular due to its excellent taste and rapid growth (Zhang Song et al, 2008). The parents of the method are the offspring of the rectus Carassius auratus and the Xingguo red carp respectively, and the fertilized eggs are cultivated by heterosperms and gynogenesis, so that the method has the advantages of strong adaptability, wide eating range, hypoxia resistance, strong disease resistance, delicious meat quality and the like, and is a crucian (Wang Aimin and the like) which grows fast, has high yield and is easy to cultivate.
In recent years, along with the continuous increase of market demands, the cultivation scale of the carassius auratus gibelio is continuously enlarged, a great number of disease problems are brought to the high-density cultivation, the disease resistance of the carassius auratus gibelio is reduced due to the degradation of germplasm and the deterioration of cultivation water quality, and particularly, the carassius auratus gibelio's ' overwintering syndrome ' is greatly exploded in the spring of 2019, so that the carassius auratus gibelio generates a great number of scabies to further cause skin ulcer, intestinal ascites and hepatomegaly, and great economic loss is caused to a raiser.
For the reason of the 'overwintering syndrome' of the carassius auratus gibelio, researches show that the bacteria which are separated at present are mainly aeromonas hydrophila, aeromonas salmonicida, aeromonas verrucosa, shewanella putrefying and pseudomonas aeruginosa, how to prevent the 'overwintering syndrome' from occurring from the source, improve the autoimmune power of the carassius auratus gibelio and strengthen the stress resistance, thereby resisting the invasion of the 'overwintering syndrome', and becoming the direction of researches of a plurality of students.
Disclosure of Invention
The invention aims to provide a Chinese herbal medicine composition for preventing the 'overwintering syndrome' of carassius auratus gibelio.
Another object of the present invention is to provide the preparation and use of the herbal composition.
The technical scheme of the invention is as follows:
a Chinese herbal medicine composition for preventing the 'overwintering syndrome' of carassius auratus gibelio consists of the following components: 15-20 parts of common andrographis herb, 15-20 parts of coptis root, 10-15 parts of radix isatidis, 8-10 parts of virgate wormwood herb, 4-6 parts of chrysanthemum and 4-6 parts of liquorice.
The Chinese herbal medicine composition preferably comprises the following components: 15 parts of common andrographis herb, 20 parts of coptis chinensis, 15 parts of radix isatidis, 8 parts of virgate wormwood herb, 6 parts of chrysanthemum and 6 parts of liquorice.
The invention relates to an application of a Chinese herbal medicine composition in preparation of a preparation for preventing the 'overwintering syndrome' of carassius auratus gibelio.
The preparation method of the Chinese herbal medicine composition for preventing the carassius auratus gibelio overwintering syndrome comprises the steps of firstly coarsely crushing the components of the Chinese herbal medicine composition, then crushing the components by an ultrafine crusher, sieving the crushed components by a 100-mesh sieve, and uniformly mixing the crushed components in a mixer to prepare the Chinese herbal medicine composition.
The invention relates to an application of a Chinese herbal medicine composition in preparing a feed for preventing the overwintering syndrome of carassius auratus gibelio.
A feed for preventing the overwintering syndrome of carassius auratus gibelio contains the Chinese-medicinal composition for preventing the overwintering syndrome of carassius auratus gibelio.
The carassius auratus gibelio feed is preferably obtained by adding the Chinese herbal medicine composition into conventional carassius auratus gibelio basic feed according to the amount of 1 weight percent.
The beneficial effects are that:
the invention starts from the immune mechanism of fish, and aims at the lesions such as liver lesions, intestinal ascites, body surface ulcers and the like which occur when the carassius auratus gibelio generates the overwintering syndrome, the common andrographis herb, coptis root, radix isatidis, herba artemisiae, chrysanthemum and liquorice are screened from Chinese herbal medicines and proportioned according to the proportion.
Herba Andrographitis is a monarch, and has effects in invigorating spleen and replenishing qi, coordinating intestinal tract, promoting enterotoxin removal, and enhancing immunity.
Rhizoma coptidis is used as a ministerial drug for purging pathogenic fire and removing toxin, mainly treating wet sores, resisting inflammation and bacteria and promoting healthy development of intestinal tracts.
The radix isatidis is also ministerial, can clear heat, relieve sore throat, detoxify and reduce swelling, and has antiviral and anti-inflammatory effects.
Herba Artemisiae Scopariae has effects of removing liver and gallbladder, repairing liver injury, and increasing cholic acid and bilirubin discharge.
Flos Chrysanthemi is also adjuvant, and can increase the number of fish monocytes and lymphocytes, promote immunity and improve
Glycyrrhrizae radix has effects of invigorating spleen and replenishing qi, regulating middle energizer and relieving urgency, and harmonizing the original drugs.
Through the use of the mixture ratio of the Chinese herbal medicines, the body antioxidant and anti-stress capacity of the carassius auratus gibelio can be improved, the intestinal bacteria content of the carassius auratus gibelio can be obviously reduced, the body repair can be quickened, the liver can be protected, and the incidence rate of the carassius auratus gibelio overwintering syndrome can be reduced.
The Chinese herbal medicine provided by the invention is simple and easy to obtain, low in cost, stable in effect and simple in preparation and use methods.
Animal experiments show that the composition can improve the immunity of fish bodies, resist multiple bacterial infection and reduce the incidence of the winter syndrome of the carassius auratus gibelio.
Detailed Description
Example 1
Taking 15 parts by weight of common andrographis herb, 15 parts by weight of coptis chinensis, 10 parts by weight of radix isatidis, 8 parts by weight of virgate wormwood herb, 4 parts by weight of chrysanthemum and 4 parts by weight of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Example 2
15 parts of common andrographis herb, 20 parts of coptis root, 15 parts of radix isatidis, 10 parts of virgate wormwood herb, 6 parts of chrysanthemum and 6 parts of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Example 3
Taking 20 parts of common andrographis herb, 18 parts of coptis chinensis, 12 parts of radix isatidis, 8 parts of virgate wormwood herb, 5 parts of chrysanthemum and 5 parts of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Example 4
18 parts of common andrographis herb, 18 parts of coptis root, 14 parts of radix isatidis, 10 parts of virgate wormwood herb, 4 parts of chrysanthemum and 4 parts of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Example 5
Taking 20 parts of common andrographis herb, 20 parts of coptis chinensis, 11 parts of radix isatidis, 10 parts of virgate wormwood herb, 6 parts of chrysanthemum and 5 parts of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Example 6
18 parts of common andrographis herb, 20 parts of coptis root, 14 parts of radix isatidis, 10 parts of virgate wormwood herb, 5 parts of chrysanthemum and 5 parts of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Comparative example 1
Taking 15 parts by weight of common andrographis herb, 15 parts by weight of coptis chinensis, 10 parts by weight of radix isatidis, 4 parts by weight of chrysanthemum and 4 parts by weight of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Comparative example 2
Taking 15 parts by weight of common andrographis herb, 15 parts by weight of coptis chinensis, 10 parts by weight of radix isatidis, 8 parts by weight of virgate wormwood herb and 4 parts by weight of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Toxicity attack comparative test: the carassius auratus gibelio is provided by the muzzle base of the fresh water aquatic institute in Jiangsu province. The test selects a carassius auratus gibelio (average body weight: 50.+ -. 0.6 g) of substantially consistent health, specification and weight. The fish feed was randomly divided into 12 groups, including 6 groups of control group and 6 groups of test group, each group being provided with 3 parallel test groups, and 20 fish per parallel group. All are put into a sterilized cement pond for feeding, the specification of the cement pond is 4m multiplied by 2m, a control group 1 is fed with a carassius auratus gibelio basal feed produced by Nanjing Shuaifeng feed limited company, a control group 2 is added with a Chinese herbal medicine compound immunopotentiator disclosed in an example 1 of CN101549029A, a control group 3 is added with a Chinese herbal medicine compound immunopotentiator disclosed in an example 2 of CN102274383B, a control group 4 is added with an immunopotentiator disclosed in an example 1 of CN101720869A, a control group 5 is added with a composition prepared in a comparative example 1, control group 6 the composition prepared in comparative example 2 was added, the addition amounts of the control group 2-immunopotentiator were consistent with the patent publication, the test groups 1-6 and the control groups 5-6 were fed with the Chinese herbal medicine compositions prepared in examples 1-6 and comparative examples 1-2, respectively, which were added in 1wt% in the basic feed, respectively, and after 14d of the Carassius auratus gibelio, the feeding test was performed for 60d, and then, were intraperitoneally injected with Aeromonas hydrophila, aeromonas salmonicida, aeromonas veronii,Multiple bacteria mixed culture solution of Shewanella putrefying and Pseudomonas aeruginosa, all bacteria are isolated from Carassius auratus producing "overwintering syndrome", and physiological saline is diluted to 1×10 5 The injection quantity is 0.6mL per 100g of fish body weight, the batch feeding is continued for 30d, the infection condition of the carassius auratus gibelio is observed and recorded, then 10 fish are randomly extracted per parallel group, and the average value of various indexes of serum immune enzyme and the total number of intestinal colony per gram are measured.
TABLE 1
The results in table 1 show that: compared with the control group 1-4, the test group 1-6 obviously improves the activity of lysozyme and superoxide dismutase, reduces the infection rate and the total number of intestinal bacteria, the control group 2-4 has no obvious immune protection effect under the condition of multiple bacterial infection of the carassius auratus gibelio, and the control group 5-6 has the same main medicine as the invention, but the result shows that the main medicine lacks virgate wormwood or chrysanthemum, the activity of lysozyme and superoxide dismutase is obviously reduced, and the total number of intestinal bacteria is obviously improved. Therefore, the Chinese herbal medicine composition can obviously improve the immunity and the antioxidation capability of the carassius auratus gibelio, and provides a theoretical basis for preventing the overwintering syndrome.
Example 7
15 parts of common andrographis herb, 20 parts of coptis root, 15 parts of radix isatidis, 8 parts of virgate wormwood herb, 6 parts of chrysanthemum and 6 parts of liquorice; the components are firstly coarsely crushed, then crushed by an ultrafine crusher, screened by a 100-mesh screen, and then fully and uniformly mixed in a mixer to prepare the Chinese herbal medicine composition.
Selecting 5 breeding bases with higher incidence rate of 'overwintering syndrome' of carassius auratus gibelio in 2019-2011 of Dafengyang county, selecting 2 breeding ponds in each breeding base, setting a control group and a test group, wherein 'overwintering syndrome' of carassius auratus gibelio occurs in 2011 of the breeding ponds and the incidence rate reaches 35% -63%, selecting breeding ponds with no 'overwintering syndrome' from the pump ponds of the freshwater aquatic institute of Jiangsu province from the breeding ponds of the carassius auratus gibelio, feeding the basic feed of carassius auratus gibelio produced by Nanshua feed limited company in control groups 1-5, selecting the Chinese herbal medicine composition prepared in the embodiment in test group 1-3, selecting the Chinese herbal medicine composition in comparative example 1 in test group 4, and selecting the Chinese herbal medicine composition in comparative example 2 in test group 5; all the Chinese herbal medicine compositions of the test groups are added into basic feed according to 1 weight percent for feeding, the test period is 6 months, 100 carassius auratus gibelio is randomly selected from each breeding pond mouth after the test is finished, haemolymph sampling is carried out, the average value of nonspecific immune enzymes is analyzed, and the incidence rate of the overwintering syndrome is checked.
TABLE 2
The results show that: compared with the control group 1-3, the test groups 1-3 obviously improve the content of lysozyme and superoxide dismutase; compared with the control group 1, the incidence of the overwintering syndrome of the test group 1 is reduced from 46% to 24%; compared with the control group 2, the incidence rate of the overwintering syndrome of the test group 2 is reduced from 39% to 21%; compared with the control group 3, the incidence of the overwintering syndrome of the test group 3 is reduced from 29% to 11%, the test group 4 and the test group 5 lack herba artemisiae scopariae and chrysanthemum, and the content of lysozyme and superoxide dismutase is increased relative to the control group 4 and the control group 5, but the incidence is not obviously reduced, so that the herba artemisiae scopariae and the chrysanthemum play an important role in the Chinese herbal medicine composition; the Chinese herbal medicine composition can obviously improve the anti-stress and anti-oxidation capacity of the carassius auratus gibelio through a comparison test and a pond mouth clinical test, and finally can reduce the incidence of the carassius auratus gibelio overwintering syndrome, which indicates that the Chinese herbal medicine composition can prevent the carassius auratus gibelio overwintering syndrome.
Claims (7)
1. A Chinese herbal medicine composition for preventing the 'overwintering syndrome' of carassius auratus gibelio is characterized by comprising the following components: 15-20 parts of common andrographis herb, 15-20 parts of coptis root, 10-15 parts of radix isatidis, 8-10 parts of virgate wormwood herb, 4-6 parts of chrysanthemum and 4-6 parts of liquorice.
2. The herbal composition of claim 1, characterized by comprising the following components: 15 parts of common andrographis herb, 20 parts of coptis chinensis, 15 parts of radix isatidis, 8 parts of virgate wormwood herb, 6 parts of chrysanthemum and 6 parts of liquorice.
3. Use of a herbal composition according to any one of claims 1 to 2 for the preparation of a formulation for the prevention of the "overwintering syndrome" of carassius auratus gibelio.
4. A preparation method of a Chinese herbal medicine composition for preventing the winter syndrome of the carassius auratus gibelio is characterized in that the Chinese herbal medicine composition for preventing the winter syndrome of the carassius auratus gibelio is prepared by coarsely crushing the components of the Chinese herbal medicine composition according to claim 1 or 2, crushing the components by an ultrafine crusher, sieving the crushed components by a 100-mesh sieve, and uniformly mixing the crushed components in a mixer.
5. Use of a herbal composition according to any one of claims 1-2 for the preparation of a feed for the prevention of the "overwintering syndrome" of carassius auratus gibelio.
6. A feed for preventing the overwintering syndrome of a carassius auratus gibelio, which is characterized by comprising the Chinese herbal medicine composition for preventing the overwintering syndrome of the carassius auratus gibelio according to claim 1 or 2.
7. The feed for carassius auratus gibelio according to claim 6, wherein the feed for carassius auratus gibelio is obtained by adding the Chinese herbal composition according to claim 1 or 2 to a conventional basic feed for carassius auratus gibelio in an amount of 1 wt%.
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| CN103405512A (en) * | 2013-08-29 | 2013-11-27 | 天津生机集团股份有限公司 | Chinese medicine granule for preventing and treating hemorrhage of grass carp, and preparation method thereof |
| CN104186951A (en) * | 2014-09-17 | 2014-12-10 | 盐城工学院 | Chinese herbal medicine feed additive for promoting growth of allogynogenetic crucian carp |
| CN105454659A (en) * | 2015-11-24 | 2016-04-06 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Carp pond culture method by use of Chinese herbal medicine |
| CN109908243A (en) * | 2019-04-03 | 2019-06-21 | 江苏省淡水水产研究所 | It is a kind of to prevent sick Chinese medicine immunity enhancer of Eriocheir sinensis " the flat son of water " and its preparation method and application |
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| CN103405512A (en) * | 2013-08-29 | 2013-11-27 | 天津生机集团股份有限公司 | Chinese medicine granule for preventing and treating hemorrhage of grass carp, and preparation method thereof |
| CN104186951A (en) * | 2014-09-17 | 2014-12-10 | 盐城工学院 | Chinese herbal medicine feed additive for promoting growth of allogynogenetic crucian carp |
| CN105454659A (en) * | 2015-11-24 | 2016-04-06 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Carp pond culture method by use of Chinese herbal medicine |
| CN109908243A (en) * | 2019-04-03 | 2019-06-21 | 江苏省淡水水产研究所 | It is a kind of to prevent sick Chinese medicine immunity enhancer of Eriocheir sinensis " the flat son of water " and its preparation method and application |
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| 水产动物应激的营养调控;芦洪梅 等;《饲料工业》;第31卷(第24期);59-62 * |
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