CN109908243A - It is a kind of to prevent sick Chinese medicine immunity enhancer of Eriocheir sinensis " the flat son of water " and its preparation method and application - Google Patents
It is a kind of to prevent sick Chinese medicine immunity enhancer of Eriocheir sinensis " the flat son of water " and its preparation method and application Download PDFInfo
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- CN109908243A CN109908243A CN201910266684.2A CN201910266684A CN109908243A CN 109908243 A CN109908243 A CN 109908243A CN 201910266684 A CN201910266684 A CN 201910266684A CN 109908243 A CN109908243 A CN 109908243A
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
Prevent sick Chinese medicine immunity enhancer of Eriocheir sinensis " the flat son of water " and its preparation method and application the invention discloses a kind of.It takes rheum officinale, Radix Astragali, Radix Isatidis, oriental wormwood, Radix Glycyrrhizae and betel nut according to a certain ratio, is crushed after first coarse crushing through micronizer, after sieving with 100 mesh sieve net, be uniformly mixed, powdered immunopotentiator is made.Chinese herbal medicine of the present invention is simple and easy to get, low in cost, effect stability, and method of preparation and use is simple.By improving Eriocheir sinensis resisting stress and oxidation resistance to improve its non-specific immunity, accelerate body injury reparation, finally prevents the generation of Eriocheir sinensis " the flat son of water " disease.
Description
Technical field
The present invention relates to aquiculture disease prevention and control fields, and in particular to a kind of to prevent in Eriocheir sinensis " the flat son of water " disease
Herbal medicine immunopotentiator and its preparation method and application.
Background technique
Eriocheir sinensis (Eriocheir sinensis) is also known as river crab, clear water crab and steamed crab, is under the jurisdiction of arthropod
Door (Arthropoda) Crustachia (Cmstacea) Decapoda (Decapoda) Gong Xie section (Varunidae) Eriocheir
(Eriocheir), delicious meat and nutritive value is higher is a kind of important economic species of aquaculture, by consumer
Like.
In recent years, with the continuous growth of the market demand, Eriocheir sinensis scale constantly expands, high-density breeding
A large amount of disease problem is brought simultaneously, the degeneration of germplasm and the deterioration of cultivation water have resulted in Eriocheir sinensis premunition
Decline, the great outburst of especially 2015 Jiangsu Province Eriocheir sinensis " the flat son of water " diseases causes ghost, hepatopancrease to bleach, water
Change and downright bad, causes great economic loss to raiser.
For the reason of Eriocheir sinensis " the flat son of water " disease, opinions vary, helminth, virus or bacterium infection, pesticide
And insecticide uses etc., is all more pathogenesis of disputing at present, traditional infringement for resisting disease, dependent on a large amount of
Antibiotic and disinfectant use, not only cause environmental pollution, it is also quite conceivable to the generation of drug resistance is caused, how from source
The generation for preventing " the flat son of water " disease on head, improves Eriocheir sinensis autoimmunity, enhances resisting stress, " water is flat to resist
The infringement of son " disease has become the direction of numerous scholar's researchs.
Shellfish is immunized, and dependent on the nospecific immunity of body, is mainly reflected in oxidation resistance and resists and answer
Swash the various nospecific immunity enzymes in reaction, at present common Testing index be glutamic-pyruvic transaminase (ALT), lysozyme (LZM),
Superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), by exempting to Eriocheir sinensis non-specificity
A kind of Chinese medicine immunity enhancer is developed in the measurement of epidemic disease enzyme, to improve Eriocheir sinensis immunity of organisms, resists " the flat son of water "
The infringement of disease, provides foundation to improve feed formula, the final long-term stability development for promoting Eriocheir sinensis industry.
Use Chinese herbal medicine less as the report of shell-fish additive in aquaculture at present, as CN103494038 is disclosed
A kind of Procambius clarkii resists the Chinese medicine immunity enhancer of white spot syndrome, which mainly improves
The immunity of organisms of Procambius clarkii to resist leukodermal generation, for object be Procambius clarkii；
CN102389499A discloses a kind of Chinese medicine extract for preventing and treating aquaculture Disease, for object be prawn and
Fish, and both herbal composites are explicitly for virus infection, and to caused by this unknown cause of Eriocheir sinensis
Disease problem, effect is unobvious, and the object being directed to and Eriocheir sinensis belong to not equal biology on taxology, to same
The absorption of kind drug is different, and target spot that drug plays a role etc. may also be different, utilizes CN103494038 and CN102389499A
Immunopotentiator disclosed in disclosed herbal composite and this patent is cooked comparative test, finds the Chinese medicine of the two patent disclosures
Composition is unobvious to Eriocheir sinensis immunity of organisms improvement effect.At present to rheum officinale, Radix Astragali, Radix Isatidis, oriental wormwood, Radix Glycyrrhizae
And betel nut compatible use and its on Eriocheir sinensis influence have not been reported, therefore, exempted from by Eriocheir sinensis non-specificity
The research of the sick death condition of the measurement and generation " the flat son of water " of epidemic disease enzyme, filters out and promotes Eriocheir sinensis nospecific immunity function
The Chinese medicine immunity enhancer of energy, to prevent the generation of Eriocheir sinensis " the flat son of water " disease, to Eriocheir sinensis aquaculture
Development is of great significance.
Summary of the invention
The object of the present invention is to provide a kind of herbal composites for preventing Eriocheir sinensis " the flat son of water " disease.
It is a further object of the present invention to provide the preparation methods of the herbal composite.
Is applied it is yet another object of the invention to provide the composition
Technical scheme is as follows:
A kind of herbal composite preventing Eriocheir sinensis " the flat son of water " disease, is made of the following components: rheum officinale 25-40 weight
Measure part, Radix Astragali 15-25 parts by weight, Radix Isatidis 15-25 parts by weight, oriental wormwood 10-20 parts by weight, Radix Glycyrrhizae 10-20 parts by weight, betel nut 4-
6 parts by weight.
The herbal composite is preferably made of the following components: rheum officinale 30-40 parts by weight, Radix Astragali 15-22 parts by weight,
Radix Isatidis 15-20 parts by weight, oriental wormwood 10-20 parts by weight, Radix Glycyrrhizae 12-20 parts by weight, betel nut 4-6 parts by weight.
Application of the herbal composite of the present invention in the preparation of preparation prevention Eriocheir sinensis " the flat son of water " disease.
The preparation method of the Chinese medicine immunity enhancer of prevention Eriocheir sinensis " the flat son of water " disease of the present invention, by institute
The Chinese medicine immunity enhancer each component elder generation coarse crushing stated is crushed through micronizer again, after sieving with 100 mesh sieve net, in mixing machine
It is uniformly mixed, that is, the immunopotentiator is made.
The Chinese medicine immunity enhancer of prevention Eriocheir sinensis " the flat son of the water " disease prepared according to the method described above.
Application of the herbal composite of the present invention in the preparation that preparation improves Eriocheir sinensis immunity.
A kind of Chinese eriocheir sinensises feed preventing Eriocheir sinensis " the flat son of water " disease, contains anti-Sinensis of the present invention
The Chinese medicine immunity enhancer of chela crab " the flat son of water " disease.
The Chinese medicine immunity enhancer is preferably added to often by the Chinese eriocheir sinensises feed according to the amount of 1wt%
Advise gained in Eriocheir sinensis basal feed.
The utility model has the advantages that
The present invention occurs when " the flat son of water " disease occurs for Eriocheir sinensis from the immune mechanism of shellfish
Hepatopancrease syndrome, sick crab Pancreas Glutamate-Pyruvate Transaminase Vigor is extremely significant to be higher than healthy crab (Yang Zongying；Zeng Liugen；Wang Yulan；Yao Yi；Marquis
It is pure as jade；Lei little Qing；Xu Liangqing；Xiong Chunxian；Yang Xianle；；Eriocheir sinensis " the flat son of water " disease Ultrastructural pathology and pathologic, physiologic research
[J]；Zhejiang Agriculture journal；07 phase in 2018), rheum officinale, Radix Astragali, Radix Isatidis, oriental wormwood, Radix Glycyrrhizae and Bin are filtered out from Chinese herbal medicine
It is bulky, and proportionally matched, rheum officinale and Radix Astragali mutually make, and can improve immunity of organisms, Radix Isatidis can be clear with tonifying middle-Jiao and Qi
Thermal detoxification play antiviral efficacy, oriental wormwood clearing liver gallbladder, Radix Glycyrrhizae liver protecting and removing toxic substances purging intense heat, betel nut can expelling parasites and food tagnancy, pass through this
Several Chinese herbal medicines proportion uses, and can be improved that Eriocheir sinensis body is anti-oxidant and anti-stress ability, significantly reduces Gu Bingzhuan ammonia
The content of enzyme, accelerates body reparation, and liver protecting reduces the generation of Eriocheir sinensis " the flat son of water " disease.
Chinese herbal medicine of the present invention is simple and easy to get, low in cost, effect stability, and method of preparation and use is simple.
Specific embodiment
Embodiment 1
Take 25 parts of rheum officinale weight, 15 parts of astragalus weight, 25 parts by weight of Radix Isatidis, 12 parts by weight of oriental wormwood, 15 parts by weight of Radix Glycyrrhizae,
4 parts by weight of betel nut；It will be crushed after the coarse crushing of above-mentioned each component elder generation through micronizer, net sieved with 100 mesh sieve, then in mixing machine
It mixes well, immunopotentiator is made.
Embodiment 2
Take 35 parts by weight of rheum officinale, 20 parts by weight of Radix Astragali, 20 parts by weight of Radix Isatidis, 18 parts by weight of oriental wormwood, 10 parts by weight of Radix Glycyrrhizae,
5 parts by weight of betel nut；It will be crushed after the coarse crushing of above-mentioned each component elder generation through micronizer, net sieved with 100 mesh sieve, then in mixing machine
It mixes well, immunopotentiator is made.
Embodiment 3
Take 40 parts by weight of rheum officinale, 18 parts by weight of Radix Astragali, 18 parts by weight of Radix Isatidis, 10 parts by weight of oriental wormwood, 20 parts by weight of Radix Glycyrrhizae,
5 parts by weight of betel nut；It will be crushed after the coarse crushing of above-mentioned each component elder generation through micronizer, net sieved with 100 mesh sieve, then in mixing machine
It mixes well, immunopotentiator is made.
Embodiment 4
Take 28 parts by weight of rheum officinale, 22 parts by weight of Radix Astragali, 16 parts by weight of Radix Isatidis, 16 parts by weight of oriental wormwood, 18 parts by weight of Radix Glycyrrhizae,
4 parts by weight of betel nut；It will be crushed after the coarse crushing of above-mentioned each component elder generation through micronizer, net sieved with 100 mesh sieve, then in mixing machine
It mixes well, immunopotentiator is made.
Embodiment 5
Take 30 parts by weight of rheum officinale, 15 parts by weight of Radix Astragali, 20 parts by weight of Radix Isatidis, 20 parts by weight of oriental wormwood, 12 parts by weight of Radix Glycyrrhizae,
6 parts by weight of betel nut；It will be crushed after the coarse crushing of above-mentioned each component elder generation through micronizer, net sieved with 100 mesh sieve, then in mixing machine
It mixes well, immunopotentiator is made.
Embodiment 6
Take 30 parts by weight of rheum officinale, 20 parts by weight of Radix Astragali, 20 parts by weight of Radix Isatidis, 15 parts by weight of oriental wormwood, 16 parts by weight of Radix Glycyrrhizae,
5 parts by weight of betel nut；It will be crushed after the coarse crushing of above-mentioned each component elder generation through micronizer, net sieved with 100 mesh sieve, then in mixing machine
It mixes well, immunopotentiator is made.
Comparative test: Eriocheir japonica sinensis is raised middle base by Jiangsu Fresh Water Aquatic Product Inst. and is provided.Test selection health, rule
Lattice and the almost the same young crab (average weight: 9.62 ± 0.05g) of weight.4 groups are randomly divided into, every group sets 2 parallel test groups,
50 test crabs of every parallel group.The cement pit for being all put in sterilized mistake is raised, and cement pit specification is 4m × 4m × 2m,
Control group 1 feeds Eriocheir sinensis basal feed (crude protein 38%, the crude fat produced by Nanjing Shuaifeng Feed Co., Ltd.
5.2%, crude fibre 6.9%, coarse ash 17.8%, total phosphorus 1.2%, lysine 2.1%), control group 2 (adds in basal feed
Add 1.0% rheum officinale), control group 3 (adds 0.5% rheum officinale and 0.5% Radix Astragali) in basal feed, and control group 4 adds
The Chinese medicine immunity enhancer of CN103494038 embodiment 1, control group 5 add No. 3 Chinese herbal medicines disclosed in CN102389499A and mention
Liquid is taken, control group 4 and 5 Chinese herbal and crude drugs preparations additive amount of control group are consistent with its patent disclosure, and test group 1-6 is fed respectively
According to the Chinese medicine immunity enhancer of the embodiment 1-6 preparation of 1wt% addition in basal feed, river crab carries out after 7 days raise and train
Test is fed, its immuno-enzymatic indices average value is measured after 90 days.
Table 1
The result shows that: compared with control group 1-5, test group 1-6 significantly improve lysozyme, super superoxide dismutase,
The content of catalase and peroxidase, and the content of glutamic-pyruvic transaminase is reduced, 5 pairs of China of control group 4 and control group
Eriocheir immunity of organisms improvement effect is unobvious, and therefore, Chinese medicine immunity enhancer of the present invention can significantly improve Eriocheir sinensia
Crab is immunized and oxidation resistance, provides theoretical basis for 7 clinical practice of subsequent embodiment.
Embodiment 7
Take 35 parts by weight of rheum officinale, 20 parts by weight of Radix Astragali, 20 parts by weight of Radix Isatidis, 15 parts by weight of oriental wormwood, 15 parts by weight of Radix Glycyrrhizae,
5 parts by weight of betel nut will crush through micronizer after the coarse crushing of above-mentioned each component elder generation, net be sieved with 100 mesh sieve, then in mixing machine
It mixes well, immunopotentiator is made.
Select the Xinghua City higher cultivation base 3 of Eriocheir sinensis " the flat son of water " sick disease incidence in 2015, each cultivation
2 culture pond mouths of base selection, if control group and test group, every year Eriocheir sinensis " the flat son of water " occurred for culture pond mouth 2015
Sick and disease incidence reaches 45%-53%, and Eriocheir japonica sinensis seed raises middle base selected from Jiangsu Fresh Water Aquatic Product Inst., and never
The breeding pool mouthful of river crab " the flat son of water " disease occurred, control group selects the Eriocheir sinensia produced by Nanjing Shuaifeng Feed Co., Ltd.
Crab basal feed is fed, and is tested group selection Chinese medicine immunity enhancer prepared by the present invention, is added to basis according to 1wt%
It being fed in feed, the test period is 6 months, and each culture pond mouth selects Eriocheir sinensis 100 at random after the test,
Hemolymph sampling is carried out, analyzes nospecific immunity enzyme average value, and check the disease incidence of " the flat son of water " disease.
Table 2
The result shows that: compared with control group 1-3, test group 1-3 significantly improves lysozyme, is more than oxide disproportionation
The content of enzyme, catalase and peroxidase, and reduce the content of glutamic-pyruvic transaminase；Compared with control group 1, test group 1
" the flat son of water " disease disease incidence drops to 14% by 41%；Compared with control group 2, test group 2 " the flat son of water " disease disease incidence is by 38%
Drop to 11%；；Compared with control group 3, test group 3 " the flat son of water " disease disease incidence drops to 5% by 29%；Pass through comparative test
And the pool mouthful clinical test, show that the Chinese medicine immunity enhancer can significantly improve Eriocheir sinensis resisting stress and anti-oxidant energy
Power, and finally can reduce the disease incidence of Eriocheir sinensis " the flat son of water " disease, show that the Chinese medicine immunity enhancer can be pre-
The generation of anti-Eriocheir sinensis " the flat son of water " disease.
Claims (9)
1. a kind of herbal composite for preventing Eriocheir sinensis " the flat son of water " disease, it is characterised in that be made of the following components: big
Yellow 25-40 parts by weight, Radix Astragali 15-25 parts by weight, Radix Isatidis 15-25 parts by weight, oriental wormwood 10-20 parts by weight, Radix Glycyrrhizae 10-20 weight
Part, betel nut 4-6 parts by weight.
2. herbal composite according to claim 1, it is characterised in that be made of the following components: rheum officinale 30-40 weight
Part, Radix Astragali 15-22 parts by weight, Radix Isatidis 15-20 parts by weight, oriental wormwood 10-20 parts by weight, Radix Glycyrrhizae 12-20 parts by weight, betel nut 4-6
Parts by weight.
3. herbal composite according to any one of claims 1 to 2 is in preparation prevention Eriocheir sinensis " the flat son of water " disease
Application in preparation.
4. a kind of preparation method for the Chinese medicine immunity enhancer for preventing Eriocheir sinensis " the flat son of water " disease, it is characterised in that will weigh
Benefit require 1 or 2 described in the coarse crushing of herbal composite each component elder generation crushed again through micronizer, after sieving with 100 mesh sieve net,
It is uniformly mixed in mixing machine, that is, the Chinese medicine immunity enhancer of described prevention Eriocheir sinensis " the flat son of the water " disease is made.
Increase 5. the Chinese herbal medicine of prevention Eriocheir sinensis " the flat son of water " disease of preparation method preparation is immune according to claim 4
Strong agent.
6. the preparation that herbal composite according to any one of claims 1 to 2 improves Eriocheir sinensis immunity in preparation
In application.
7. Chinese medicine immunity enhancer described in claim 5 is preparing the application in Chinese eriocheir sinensises feed.
8. a kind of Chinese eriocheir sinensises feed for preventing Eriocheir sinensis " the flat son of water " disease, it is characterised in that contain claim 5 institute
The Chinese medicine immunity enhancer of prevention Eriocheir sinensis " the flat son of the water " disease stated.
9. Chinese eriocheir sinensises feed according to claim 8, it is characterised in that the Chinese eriocheir sinensises feed is will to weigh
Benefit require 5 described in Chinese medicine immunity enhancer according to the amount of 1wt% be added in conventional Eriocheir sinensis basal feed gained.
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|---|---|---|---|
| CN201910266684.2A CN109908243B (en) | 2019-04-03 | 2019-04-03 | Chinese herbal medicine immunopotentiator for preventing Chinese mitten crab 'shrivelled crab' disease and preparation method and application thereof |
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| CN201910266684.2A CN109908243B (en) | 2019-04-03 | 2019-04-03 | Chinese herbal medicine immunopotentiator for preventing Chinese mitten crab 'shrivelled crab' disease and preparation method and application thereof |
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Cited By (1)
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| CN115252681A (en) * | 2022-07-27 | 2022-11-01 | 江苏省淡水水产研究所 | Chinese herbal medicine composition for preventing carassius auratus gibelio overwintering syndrome as well as preparation and application thereof |
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Cited By (2)
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| CN115252681B (en) * | 2022-07-27 | 2023-06-23 | 江苏省淡水水产研究所 | Chinese herbal medicine composition for preventing carassius auratus gibelio overwintering syndrome and preparation and application thereof |
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