CN109452495A - A kind of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive and the preparation method and application thereof - Google Patents
A kind of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive and the preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/83—Thymelaeaceae (Mezereum family), e.g. leatherwood or false ohelo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additives and the preparation method and application thereof, which includes following components: Wikstroemia indica, Fructus Aurantii, licorice, the seed of feather cockscomb, watermelon peel, dandelion, raw hawthorn, Radix Codonopsis, salt, carrier;Preparation method is to put into each component in mixing machine, is sent into micronizer and crushes after being sufficiently mixed, and smashed material is sieved to get prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive.Additive of the invention is added in freshwater crayfish feed stuff, it can be improved the immune and oxidation resistance of Procambius clarkii, raising stress, enhance disease resistance, the generation for preventing and treating Procambius clarkii spiral shell substance disease, the feed for being applicable in this feed premix material additive does not need addition antibiont drug, and Procambius clarkii product produced is nuisanceless green environmental protection food.
Description
Technical field
The invention belongs to aquatic product matched field of feed additive technology, and in particular to a kind of prevention and treatment Procambius clarkii spiral shell substance
Sick Chinese herbal feed additive and the preparation method and application thereof.
Background technique
In recent years, Procambius clarkii Procambarus clarkii is propagated artificially in the ascendant throughout the country.According to system
Meter, Jiangsu, many counties in Hubei cultivation scale just have reached tens of thousands of mus.But with expanding rapidly for artificial breeding area, disease
Harmful generation is also more and more frequent and severe.The country has reported some diseases of Procambius clarkii in succession, these diseases are
To Procambius clarkii, culturist brings heavy economic losses.
Spiral shell substance is that a kind of individual is minimum, form is changeable, the very Special Bacteria of not no cell wall, and the 1970s is first
Secondary to find in plant and insect bodies, some are the pathogenic bacteria of crops (corn, citrus etc.) and beneficial insect (honeybee).From trouble
The spiral shell substance being separated in the Eriocheir sinensis of " tremble disease " is the novel cause of disease found from aquatic crustacean for the first time, life
Entitled Eriocheir sinensis spiral shell substance (Spiroplasma eriocheiris sp.Nov), it is the pathogenic bacteria of " tremble disease ".This
People are expanded to waters by land to the distribution of spiral shell substance by one discovery.In addition to river crab, spiral shell substance is to other economic aquatic crusts
Animal also has extensive infectivity, such as also discovery in succession in Procambius clarkii, Penaeus Vannmei, Macrobrachium rosenbergii, Macrobrachium nipponensis
Spiral shell substance.It is ground through what molecular biology, immune analysis, cross-infection experiment and Ultrastructural Pathology feature compared etc.
Study carefully, the spiral shell substance for finally determining these different hosts sources is one species with the spiral shell substance for causing river crab " tremble disease ", is shown
This kind of spiral shell substance can carry out cross-infection and propagation between different aquatic crustacean species.
After Chinese herbal medicine compatibility, the specificity of body and the function of non-specific immunity not only can be improved;Also have anti-
The effect of bacterium, inhibition virus etc..In aquaculture, but various chemical synthetic drugs and hormone long-term or largely make
With aquatic livestock can develop drug resistance, and remain on the health that the intracorporal dose of aquatic livestock also will affect eater.And medium-height grass
Medicine additive does not have the drawbacks of chemical synthetic drug then, while having the double action of trophism and Drug concurrently.Therefore, Chinese herbal medicine
Additive has become the additive of new generation of substitution chemical synthetic drug and hormone.
It is comprehensive for the anti-hickie of Procambius clarkii about rheum officinale, Herba Epimedii, Radix Astragali, Radix Isatidis combination formula that there are reports
The report of simulator sickness, as CN103494038 B discloses a kind of anti-white spot syndrome virus Chinese herbal medicine Immune-enhancing effect of Procambius clarkii
Agent and its preparation method and application is mainly made from the following raw materials in parts by weight: 30-38 parts of rheum officinale, 28-35 parts of Herba Epimedii, yellow
15-25 parts of stilbene, 15-25 parts of Radix Isatidis.But the traditional Chinese medicinal components are mainly to raising immunity, especially anti-white spot syndrome virus
Poison has certain curative effect, which mainly has caused by white spot syndrome virus, and there is no remarkable effect to bacteriosis.Furthermore
There are also some reports about enhancing Procambius clarkii immunity, as a kind of raising Procambius clarkii juvenile prawn of CN103431244B is exempted from
The vitamin feed and additive of epidemic disease power, this report is mainly based on anti-microbial formulation, to Procambius clarkii spiral shell substance disease
Without remarkable result, a kind of Procambius clarkii of CN107996888A is at the novel disease-resistant additive of shrimp and preparation method, this report for another example
Immunity is mainly improved with polysaccharide component, improves yield, reduces the death rate, but to Procambius clarkii spiral shell substance disease also without
Remarkable result.For another example application of the CN101422469B terramycin in the drug of preparation treatment aquatic animal spiroplasmosis, the report
The spiral shell substance disease of aquatic animal is treated in road using terramycin as sole component, but sole component --- terramycin remains as antibiotics
Substance, easily lead to the intracorporal residual of aquatic livestock influences the health of the mankind again, exists with herbal control aquatic animal disease aobvious
Write difference.The current herbal control measure in relation to shellfish spiral shell substance disease has no related report.
Summary of the invention
The purpose of the present invention is the spiral shell substance diseases multiple for Procambius clarkii and shellfish, provide a kind of prevention and treatment gram
Crayfish and shellfish spiral shell substance disease Chinese herbal feed additive and the preparation method and application thereof, the additive can be with
The immunocompetence for improving Procambius clarkii and shellfish, builds up health, and reduces Procambius clarkii spiral shell substance disease and occurs, favorably
In the healthy growth of Procambius clarkii.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
A kind of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive includes following components: brother in parts by weight
12.0 parts of king 5.0-, 5.0-15.0 parts of Fructus Aurantii, 7.0-14.0 parts of licorice, 4.0-20.0 parts of the seed of feather cockscomb, watermelon peel 3.0-12.0
Part, 3.0-10.0 parts of dandelion, 8.0-20.0 parts of raw hawthorn, 5.0-20.0 parts of Radix Codonopsis, 3.0-8.0 parts of salt, carrier 10.0-
30.0 parts.
Wikstroemia indica Wikstroemia indica (Linn.) C.A.Mey, alias: wikstroemia indica, cotton skin, mountain cotton skin, Huang
Leather, cotton root, mountain beans, small drooping stringbush flower root and bark, paulownia leather, brother's spring scenery, sparrow infantile paralysis, belong to Thymelaeceae, canescent wikstroemia category shrub, there is heat-clearing
Removing toxic substances, dissipating bind are relieved oedema or abdominal distension through diuresis or purgation.For cough with lung heat, mumps, scrofula, rheumatic arthralgia, sore furuncle poison, oedema abdominal distension.
Fructus Aurantii is the drying immature fruit of rutaceae bitter orange Citrus aurantium L. and its variety.
Fructus Aurantii property is bitter, pungent, sour, warm, has the effect of regulating the flow of QI to ease the stomach, row stagnant dissipate-swelling, cures mainly the chest side of body stagnation of the circulation of vital energy, turgor pain, accumulation of food in the stomach and intes tine due to indigestion,
Stop in phlegm retention;Gastroptosis, rectal prolapse, the illnesss such as uterine prolapse.
Licorice Radix Glycyrrhizae. alias lantern, close sweet (honey is sweet), close grass, state is old, powder is careless, Herba Hedyotis cantonensis, sweet tea root
Son, is leguminous perennial herb at stick grass.Major function: invigorating the spleen and benefiting qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain is adjusted
With all medicines.For weakness of the spleen and the stomach, fatigue and weakness, shortness of breath and palpitation, coughing with a lot of sputum, stomach duct and abdomen, four limbs contraction, carbuncle sore tumefacting virus, delay
Solve drug toxicity, strong.Licorice is different from radix glycyrrhizae preparata, and radix glycyrrhizae preparata is mainly used for tonifying middle-Jiao and Qi, and for reconciling drug, also
Play the role of correcting flavour of a drug.
The seed of feather cockscomb is the dry mature seed of annual herb amaranthaceous plant feather cockscomb.Bitter, it is slightly cold.There are clearing away the liver-fire, wind-dispelling
The effect of heat, improving eyesight, blood pressure lowering.
Watermelon peel, alias EXOCARPIUM CITRULLI are the outside rind of cucurbitaceous plant watermelon.It is cool in nature, it is sweet in flavor, it is nontoxic;Enter spleen, stomach
Two warps.Clear heat is antipyretic, quenches the thirst, diuresis.For hot summer weather polydipsia, oliguresis, oedema, aphthae.Watermelon peel contains aquatic
The nutriments such as sugar needed for animal and thick protein, crude fat, crude fibre, chlorophyll, also containing carrotene, thiamine,
The mineral salts such as the vitamins such as riboflavin, niacin, ascorbic acid and calcium, phosphorus, iron.The water such as flathead, silver carp, grass, carp are fed with watermelon peel
Cultivation object is produced, palatability is good, absorption easy to digest.Therefore, using watermelon peel as aquatic animal feed raw material, feeding had both been expanded
Expect source, production cost 15%~20% can be reduced, and reduce environmental pollution.
Dandelion Taraxacum mongolicum Hand.-Mazz. is composite family, Dandelion herbaceos perennial.
With clearing heat and detoxicating, diuresis dissipating bind.Control acute mastitis, adenolymphitis, scrofula, boil poisonous soreness, acute conjunctivitis, flu hair
Heat, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections.
Raw hawthorn Crataegus pinnatifida Bunge also known as hawthorn, large-fruited Chinese hawthorn, rosaceae hawthorn have
Food digesting stomach fortifying activates blood circulation and disperses blood clots, convergence and dysentery and other effects.
Radix Codonopsis is the dry root of Campanulaceae Radix Codonopsis, codonopsis pilosula var. modesta or radix codonpsis tangshen etc..Radix Codonopsis is sweet in flavor, mild-natured.There is bowl spares
QI invigorating is quenched the thirst, strengthening spleen and tonifying lung, nourishes blood and promotes the production of body fluid.For spleen-lung Qi deficiency, deficiency of food and tireness, false asthma cough, insufficiency of vital energy and blood, sallow complexion,
Shortness of breath and palpitation, injury thirst, Heat Diabetes.Lack of desire to speak and qi, weakness of limbs, loss of appetite, the deficiency of vital energy, deficiency of qi and fluid, deficiency of qi and blood
And the diseases such as blood deficiency chlorosis.
Further, the carrier is one of wheat bran, wheat flour, rice bran or zeolite powder or a variety of mixing.
Each component is dried or is shone by the preparation method of above-mentioned prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive
It is put into mixing machine after dry, is sent into micronizer and is crushed after being sufficiently mixed, smashed material is crossed into 400-800 mesh
Sieve obtains the Chinese herbal feed additive of prevention and treatment Procambius clarkii spiral shell substance disease.
Further, the mixing velocity of mixing machine is 15-30rpm.
Further, the crushing speed of micronizer is 300-600rpm.
Application of the said herbal medicine feed addictive in the feed of preparation prevention or treatment Procambius clarkii spiral shell substance disease.
Further, additive amount of the Chinese herbal feed additive in freshwater crayfish feed stuff is 2-4kg/t.
Said herbal medicine feed addictive is in preparation abalone, scallop or raw oyster feed, or raises other shell-fish water
Produce the application on animal
Compared with the prior art, the advantages of the present invention are as follows:
1, Wikstroemia indica, Fructus Aurantii, licorice, the seed of feather cockscomb, watermelon peel, dandelion are antipyretic and antidotal type Chinese medicine, have heat-clearing solution
The effect of poison, dampness removing, astringing to arrest bleeding can be used for antibacterial, dysentery, and cold-resistant stress and murder harmful bacteria, reduce Procambius clarkii spiral shell
The generation of substance disease.
2, watermelon peel is containing there are many nutrition such as sugar and thick protein, crude fat, crude fibre, chlorophyll needed for aquatile
Substance also contains the mineral salts such as vitamins and calcium, phosphorus, iron such as carrotene, thiamine, riboflavin, niacin, ascorbic acid,
Also there is antibacterial, clearing heat and detoxicating and other effects simultaneously, can effectively reduce the generation of Procambius clarkii spiral shell substance disease, also reduce simultaneously
Feed cost.
3, licorice, raw hawthorn not only have the effect of clearing heat and detoxicating, and there are also stronger food calling appetizings to act on, in prevention and treatment gram
The generation of crayfish spiral shell substance disease can enhance ingesting for Procambius clarkii, promote growth.
4, above-mentioned medicinal material all has the effect for improving immunity, especially Procambius clarkii this kind without congenital immunity
For the animal of function, improves its immunity by ingesting and can achieve the effect that get twice the result with half the effort.
5, additive of the invention is added to salt and has certain attractant effect, increases palatability, is conducive to drop
The feed coefficient of low Procambius clarkii improves its survival rate.
6, additive of the invention can be improved the immune and oxidation resistance of Procambius clarkii, raising stress, enhancing is anti-
Sick ability, the feed for being applicable in this feed premix material additive do not need addition antibiont drug, and Procambius clarkii produced produces
Product are nuisanceless green environmental protection food.
Specific embodiment
Technical solution of the present invention is described further combined with specific embodiments below.
Embodiment 1
It prepares 20kg and prevents and treats Procambius clarkii spiral shell substance disease Chinese herbal feed additive, the composition of the additive is as follows:
Brother king 1.0kg, Fructus Aurantii 1.4kg, licorice 2.0kg, seed of feather cockscomb 2.0kg, watermelon peel 2.0kg, dandelion 1.2kg, raw hawthorn
2.4kg, Radix Codonopsis 2.0kg, salt 1.0kg, carrier 5.0kg.The carrier is wheat bran.
It takes in said components investment mixing machine, after 15r/min is sufficiently mixed, is crushed in micronizer, crush speed
Are as follows: 300r/min;Smashed material is crossed 400 mesh screens to raise to get product prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal medicine
Feed additives.Above-mentioned prevention and treatment Procambius clarkii spiral shell substance disease is added to the dedicated feeding of Procambius clarkii with Chinese herbal feed additive
Expect, 2.0kg additive is added in feed per ton.
Six mouthfuls of ponds of Procambius clarkii cultivation of Yancheng of Jiangsu Province Jiangsu Zheng Yuan Chuan Hui development in agricultural science and technology Co., Ltd, six
The area of mouth cultivating pool is 5 mu, and the pond depth of water is 1. meters or so.Current year in 2018 puts Procambius clarkii in a suitable place to breed, or so June shrimp body
Size average out to 5.7g.It cleans up the pond before experiment six mouthfuls and is cleaned up the pond disinfection with quick lime 120 kg/mu.Afterwards by Procambius clarkii seed
After being sterilized with METHIONINE AMINO-CARBOXYL IODINE COMPLEX, tested.Wherein three mouthfuls of 5 mu of shrimp pools are the control pool mouthful, are matched using conventional Procambius clarkii commodity
Feed is closed, another three mouthfuls of shrimp pools are the test pool, using (feed is emerging by Yancheng perseverance by the Procambrus clarkii special feed of above-mentioned additive
Feed corporation,Ltd).Reinforce process management in breeding process, according to weather condition, feed day 1 time, daily ration of feeding is shrimp weight
2% or so, and according to food situation, do suitable adjustment.Two months, acquire sample analysis.
Experimental result shows that the sick disease incidence of this additive group Procambius clarkii spiral shell substance disease is 0.52%, the spiral shell of control group
Substance disease disease incidence is 2.13% (p < 0.05).
Embodiment 2
It prepares 20kg and prevents and treats Procambius clarkii spiral shell substance disease Chinese herbal feed additive, the composition of the additive is as follows:
Brother king 1.2kg, Fructus Aurantii 1.6kg, licorice 1.8kg, seed of feather cockscomb 3.0kg, watermelon peel 1.6kg, dandelion 1.0kg, raw hawthorn
3.0kg, Radix Codonopsis 1.6kg, salt 1.2kg, carrier 4.0kg.The carrier is that wheat bran, wheat flour and rice bran mix.
It takes in said components investment mixing machine, after 28r/min is sufficiently mixed, is crushed in micronizer, crush speed
Are as follows: 500r/min;Smashed material is crossed into 600 mesh screens, product prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed adds
Add agent.By above-mentioned prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive.It is added to Procambrus clarkii special feed, often
3.0kg additive is added in ton feed.
The Procambius clarkii of farm, Yancheng, Jiangsu Province Funing County is 10 at 6 mouthfuls of ponds of shrimp aquaculture, the area of 6 mouthfuls of cultivating pools
Mu, the pond depth of water are 1 meter or so.Current year in 2018 puts Procambius clarkii seed in a suitable place to breed, or so June shrimp body size average out to 5.8g.
To cleaning up the pond with 150 kgs/acre of quick lime disinfections of cleaning up the pond before experiment.It is carried out after Procambius clarkii shrimp species are sterilized with METHIONINE AMINO-CARBOXYL IODINE COMPLEX afterwards
Test.Three mouthfuls of 10 mu of ponds are randomly selected as the control pool mouthful, using conventional Procambius clarkii commodity mixed feed, another three mouthfuls of ponds
To test the pool, feed addictive is added using by the freshwater crayfish feed stuff of above-mentioned formula.Reinforce process management in breeding process,
It according to weather condition, feeds day 1 time, daily ration of feeding is 2% or so of shrimp weight, and according to food situation, does suitable adjustment.
After two months, sample analysis is acquired.
The sick disease incidence of this additive group Procambius clarkii spiral shell substance disease is 0.73%, and the spiral shell substance disease disease incidence of control group is
2.55% (p < 0.05).
Embodiment 3
It prepares 20kg and prevents and treats Procambius clarkii spiral shell substance disease Chinese herbal feed additive, the composition of the additive is as follows:
Brother king 1.4kg, Fructus Aurantii 1.8kg, licorice 2.2kg, seed of feather cockscomb 2.4kg, watermelon peel 1.8kg, dandelion 1.6kg, raw hawthorn
2.6kg, Radix Codonopsis 2.4kg, salt 0.8kg, carrier 3.0kg.The carrier be wheat bran, wheat flour, rice bran and zeolite powder mixing and
At.
It takes in said components investment mixing machine, after 30r/min is sufficiently mixed, is crushed in micronizer, crush speed
Are as follows: 600r/min;Smashed material is crossed into 800 mesh screens, product prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed adds
Add agent.By above-mentioned prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive.It is added to Procambrus clarkii special feed, often
4.0kg additive is added in ton feed.
The Procambius clarkii of Jiangsu Huai'an Xuyi river agricultural development Co., Ltd cultivates 6 mouthfuls of ponds, 6 mouthfuls of cultivating pools
Area be 2 mu, the pond depth of water be 1.0 meters or so.Current year in 2018 puts Procambius clarkii seed in a suitable place to breed, or so June shrimp body size
Average out to 5.8g.It is cleaned up the pond before experiment with 150 kgs/acre of quick lime disinfections of cleaning up the pond.Afterwards by Procambius clarkii seed METHIONINE AMINO-CARBOXYL IODINE COMPLEX
It is tested after disinfection.Three mouthfuls of 2 mu of ponds are randomly selected as the control pool mouthful, using conventional Procambius clarkii commodity mixed feed,
Another three mouthfuls of ponds are the test pool, add the Chinese herbal feed using conventional feed.Reinforce process management in breeding process, according to day
Gas situation is fed 1 day, and daily ration of feeding is 2% or so of shrimp weight, and according to food situation, does suitable adjustment.Two months
Afterwards, sample analysis is acquired.
The sick disease incidence of this additive group Procambius clarkii spiral shell substance disease is 0.42%, and the spiral shell substance disease disease incidence of control group is
2.34% (p < 0.05).
To the analyses of sample other indexs, the results are shown in Table 1.
1 Chinese herbal feed additive of table is grown to Procambius clarkii and the influence of spiral shell substance disease disease incidence
Index | Control group | Chinese herbal medicine group |
Disease incidence | 2.34% ± 0.25a | 0.42 ± 0.05%b |
Original body mass (g) | 5.8±0.41 | 5.8±0.52 |
Test opisthosoma weight (g) | 19.23±1.85b | 28.75±1.58a |
Rate of body weight gain | 2.32±0.19b | 3.96±0.41a |
Note: different lowercase letter indication differences of going together are significant (P < 0.05)
After experiment, control group and Chinese herbal feed additive group Procambius clarkii blood is taken to be placed in -20 DEG C of refrigerators respectively
It saves.The kit measurement of Bioengineering Research Institute's offer is built up with Nanjing, detailed results are shown in Table 2.
Influence of 2 Chinese herbal feed additive of table to Procambius clarkii blood antioxidant enzyme activity
Index | Control group | Chinese herbal medicine group |
Superoxide dismutase/(Umg-1)SOD | 428.33±18.24b | 520.31±20.69a |
Nitric oxide (μm olL-1)NO | 40.56±4.21b | 70.52±5.25a |
Albumin (gL-1)ALB | 16.15±1.22b | 37.23±1.61a |
Lysozyme/(μ g/mL) LZM | 90.92±5.34b | 140.66±9.34a |
Alkaline phosphatase (U/L) AKP | 7.12±0.41b | 12.25+1.16a |
Acid phosphatase (U/L) (ACP) | 8.24±1.32b | 14.68±1.68a |
Total protein/(g/L) TP | 78.25±4.31b | 121.22±5.65a |
Note: different lowercase letter indication differences of going together are significant (P < 0.05)
Most important Antioxidant Indexes include SOD in shrimp body, basic function be remove superoxide radical such as super oxygen yin from
Son (O2-) and hydrogen peroxide (H2O2).The above results show that Chinese herbal medicine group significantly enhances the inoxidizability of Procambius clarkii.Blood
Nitric oxide plays a part of messenger molecule in liquid.When endothelium will be issued to muscle loosens instruction to promote blood circulation, it
Some nitric oxide molecules will be generated, these molecule very littles can easily pass through cell membrane.Circumvascular smooth muscle is thin
Born of the same parents receive diastole after signal, distend the blood vessels.The raising of albumin and total protein shows that the immunocompetence of test group is constantly mentioning
It is high.Acid phosphatase and alkaline phosphatase are the important components of phagolysosome in crustacean body, are carried out in haemocyte
It can be along with the release of acid phosphatase and alkaline phosphatase in phagocytosis and encapsulation response.There is bright ACP and AKP vigor in test group
It is aobvious to increase, show that test group Chinese herbal medicine has immunological enhancement to Procambius clarkii.
The foregoing is only a preferred embodiment of the present invention, the scope of protection of the present invention is not limited to this, it is any ripe
Know those skilled in the art within the technical scope of the present disclosure, the letter for the technical solution that can be become apparent to
Altered or equivalence replacement are fallen within the protection scope of the present invention.
Claims (8)
1. a kind of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive, which is characterized in that include in parts by weight
Following components: 5.0-12.0 parts of Wikstroemia indica, 5.0-15.0 parts of Fructus Aurantii, 7.0-14.0 parts of licorice, 4.0-20.0 parts of the seed of feather cockscomb, west
3.0-12.0 parts of melon skin, 3.0-10.0 parts of dandelion, 8.0-20.0 parts of raw hawthorn, 5.0-20.0 parts of Radix Codonopsis, salt 3.0-8.0
Part, 10.0-30.0 parts of carrier.
2. prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive according to claim 1, feature exist
In: the carrier is one or more in wheat bran, wheat flour, rice bran or zeolite powder mixes.
3. based on the preparation method of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive described in claim 1,
It is characterized by: being put into mixing machine after each component is dried or dried, it is sent into micronizer after being sufficiently mixed and carries out powder
It is broken, smashed material is crossed into 400-800 mesh screen, obtains prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive.
4. the preparation method of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive according to claim 3,
It is characterized by: the mixing velocity of mixing machine is 15-30r/min.
5. the preparation method of prevention and treatment Procambius clarkii spiral shell substance disease Chinese herbal feed additive according to claim 3,
It is characterized by: the crushing speed of micronizer is 300-600 r/min.
6. sick in preparation prevention and treatment or treatment Procambius clarkii spiral shell substance based on Chinese herbal feed additive described in claim 1
Application in feed.
7. application according to claim 6, it is characterised in that: the Chinese herbal feed additive is in freshwater crayfish feed stuff
In additive amount be 2-4kg/t.
8. the application based on Chinese herbal feed additive as claimed in claim 6 in preparation abalone, scallop or raw oyster feed.
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CN111264706A (en) * | 2020-03-06 | 2020-06-12 | 江苏敖众生物科技有限公司 | Solid mixed feed additive for livestock breeding |
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