CN115227624B - Infant skin care oil containing nut oil - Google Patents
Infant skin care oil containing nut oil Download PDFInfo
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- CN115227624B CN115227624B CN202211051884.4A CN202211051884A CN115227624B CN 115227624 B CN115227624 B CN 115227624B CN 202211051884 A CN202211051884 A CN 202211051884A CN 115227624 B CN115227624 B CN 115227624B
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- oil
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- bletilla striata
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- 239000003921 oil Substances 0.000 title claims abstract description 65
- 235000019198 oils Nutrition 0.000 title claims abstract description 65
- 235000019488 nut oil Nutrition 0.000 title claims abstract description 38
- 239000010466 nut oil Substances 0.000 title claims abstract description 38
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000000341 volatile oil Substances 0.000 claims abstract description 30
- 244000185386 Thladiantha grosvenorii Species 0.000 claims abstract description 26
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 claims abstract description 26
- 150000001875 compounds Chemical class 0.000 claims abstract description 24
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 16
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 16
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 16
- 229940046009 vitamin E Drugs 0.000 claims abstract description 16
- 239000011709 vitamin E Substances 0.000 claims abstract description 16
- 244000144725 Amygdalus communis Species 0.000 claims abstract description 13
- 235000011437 Amygdalus communis Nutrition 0.000 claims abstract description 12
- 239000008168 almond oil Substances 0.000 claims abstract description 12
- 235000019498 Walnut oil Nutrition 0.000 claims abstract description 10
- 239000008170 walnut oil Substances 0.000 claims abstract description 10
- 241001313857 Bletilla striata Species 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 238000002360 preparation method Methods 0.000 claims description 16
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- 238000005303 weighing Methods 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 9
- 238000010992 reflux Methods 0.000 claims description 8
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- 239000002537 cosmetic Substances 0.000 claims description 6
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- 208000024891 symptom Diseases 0.000 abstract description 9
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- 230000003110 anti-inflammatory effect Effects 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 description 21
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- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 14
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- VYZAHLCBVHPDDF-UHFFFAOYSA-N Dinitrochlorobenzene Chemical compound [O-][N+](=O)C1=CC=C(Cl)C([N+]([O-])=O)=C1 VYZAHLCBVHPDDF-UHFFFAOYSA-N 0.000 description 4
- 206010030113 Oedema Diseases 0.000 description 4
- 230000001154 acute effect Effects 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000019476 oil-water mixture Nutrition 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 235000014571 nuts Nutrition 0.000 description 3
- 241000723382 Corylus Species 0.000 description 2
- 235000007466 Corylus avellana Nutrition 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- URLKBWYHVLBVBO-UHFFFAOYSA-N Para-Xylene Chemical group CC1=CC=C(C)C=C1 URLKBWYHVLBVBO-UHFFFAOYSA-N 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 239000002035 hexane extract Substances 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 231100000344 non-irritating Toxicity 0.000 description 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 2
- 238000011552 rat model Methods 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000013112 stability test Methods 0.000 description 2
- RGZSQWQPBWRIAQ-CABCVRRESA-N (-)-alpha-Bisabolol Chemical compound CC(C)=CCC[C@](C)(O)[C@H]1CCC(C)=CC1 RGZSQWQPBWRIAQ-CABCVRRESA-N 0.000 description 1
- 239000001500 (2R)-6-methyl-2-[(1R)-4-methyl-1-cyclohex-3-enyl]hept-5-en-2-ol Substances 0.000 description 1
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- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 1
- 235000009025 Carya illinoensis Nutrition 0.000 description 1
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- 241000196324 Embryophyta Species 0.000 description 1
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- 241001465754 Metazoa Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- VCUFZILGIRCDQQ-KRWDZBQOSA-N N-[[(5S)-2-oxo-3-(2-oxo-3H-1,3-benzoxazol-6-yl)-1,3-oxazolidin-5-yl]methyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C1O[C@H](CN1C1=CC2=C(NC(O2)=O)C=C1)CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F VCUFZILGIRCDQQ-KRWDZBQOSA-N 0.000 description 1
- 206010033733 Papule Diseases 0.000 description 1
- 206010040844 Skin exfoliation Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- RGZSQWQPBWRIAQ-LSDHHAIUSA-N alpha-Bisabolol Natural products CC(C)=CCC[C@@](C)(O)[C@@H]1CCC(C)=CC1 RGZSQWQPBWRIAQ-LSDHHAIUSA-N 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000021302 avocado oil Nutrition 0.000 description 1
- 239000008163 avocado oil Substances 0.000 description 1
- 239000002199 base oil Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 235000020226 cashew nut Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940021231 clearskin Drugs 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
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- 239000000469 ethanolic extract Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
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- 238000000465 moulding Methods 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 229960002446 octanoic acid Drugs 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000002390 rotary evaporation Methods 0.000 description 1
- 230000036548 skin texture Effects 0.000 description 1
- 238000001256 steam distillation Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- LADGBHLMCUINGV-UHFFFAOYSA-N tricaprin Chemical compound CCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCC)COC(=O)CCCCCCCCC LADGBHLMCUINGV-UHFFFAOYSA-N 0.000 description 1
- 229940052016 turmeric extract Drugs 0.000 description 1
- 235000020240 turmeric extract Nutrition 0.000 description 1
- 239000008513 turmeric extract Substances 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
Abstract
The application discloses a baby skin care oil containing nut oil, which comprises 100 parts of compound nut oil, 1-3 parts of momordica grosvenori volatile oil and 3-8 parts of vitamin E; wherein, the mass ratio of the compound nut oil is 1:0.1 to 0.2 percent of sweet almond oil and walnut oil are uniformly mixed. The infant skin care oil prepared by the application has higher stability, can relieve eczema symptoms and has anti-inflammatory effect.
Description
Technical Field
The application relates to the field of skin care product preparation. More particularly, the present application relates to a nut oil-containing infant skin care oil.
Background
Nuts are a plant material with high oil content, common nuts comprise almonds, walnuts, cashews, hazelnuts, pecans and the like, and the oil extracted from the nuts can be applied to the fields of foods, cosmetics, skin care products and the like, such as sweet almond oil, walnut oil, hazelnuts and the like. In the prior art, sweet almond oil is often used as basic grease of skin care oil, has the magic effects of easy absorption, nourishing and moisturizing, and is convenient to use. At present, most of the existing skin care oil for infants only has the effects of moisturizing or nourishing skin, has single functions, and needs to develop more skin care oil products with safety and other effects.
Disclosure of Invention
The application aims to solve at least the problems and provide the infant skin care oil containing the nut oil, which has better treatment effect on eczema by adding the momordica grosvenori volatile oil and the bletilla striata treated substance, has a certain anti-inflammatory effect and improves the efficacy of the infant skin care oil.
To achieve these objects and other advantages and in accordance with the purpose of the application, there is provided a nut oil-containing infant skin care oil comprising the following components in parts by weight:
100 parts of compound nut oil, 1-3 parts of momordica grosvenori volatile oil and 3-8 parts of vitamin E;
wherein, the mass ratio of the compound nut oil is 1: and mixing 0.1-0.2 of sweet almond oil and walnut oil uniformly.
Preferably, the preparation of the momordica grosvenori volatile oil comprises the following steps: and (3) placing the crushed fructus momordicae in deionized water, uniformly stirring, placing at 100 ℃ and refluxing for 5-6 hours to obtain an oil-water mixed solution, and extracting and steaming the oil-water mixed solution to obtain the fructus momordicae volatile oil.
Preferably, before the crushed fructus momordicae is placed in deionized water, placing the crushed fructus momordicae in 0.2M phosphoric acid buffer solution, then placing the fructus momordicae in 45-50 ℃ for heating for 15-20 min, then placing the fructus momordicae in an ultrasonic environment for ultrasonic treatment for 15-20 min to obtain treatment solution, and then adding deionized water into the treatment solution for reflux; the mass ratio of the crushed fructus momordicae to the phosphate buffer solution is 1:2.5.
preferably, the composition further comprises 5-10 parts by weight of bletilla striata treatment substance.
Preferably, the preparation of the bletilla striata treatment product comprises the following steps: weighing crushed bletilla striata, placing the crushed bletilla striata into white oil special for cosmetics, slowly stirring the mixture at 40-45 ℃ for 12-24 hours, filtering the mixture, and collecting filtrate and cooling the filtrate to room temperature to obtain the bletilla striata treated matter; wherein, the mass ratio of the bletilla striata to the white oil is 1: 5-10.
Preferably, the skin care oil is prepared specifically as follows: and weighing the compound nut oil, the momordica grosvenori volatile oil, the vitamin E and the bletilla striata treated matter according to the proportion, and uniformly stirring at 20-25 ℃ to obtain the compound nut oil.
The application at least comprises the following beneficial effects:
firstly, the momordica grosvenori volatile oil is added, so that the eczema symptom can be effectively relieved; the treatment effect of eczema can be further improved in an auxiliary way by adding the bletilla striata treatment substance.
The skin care oil for infants is prepared from natural substances, has no other chemical additives, is safe to use, can effectively relieve eczema symptoms, has higher stability and has a certain anti-inflammatory effect.
Additional advantages, objects, and features of the application will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the application.
Detailed Description
The present application is described in further detail below with reference to examples to enable those skilled in the art to practice the same by referring to the description.
The infant skin care oil containing nut oil of the application takes the disclosed components (compound nut oil, momordica grosvenori volatile oil, vitamin E and bletilla striata treatment substance) as the basic active components, and other functional components such as moisturizing and nourishing component agents (e.g. hyaluronic acid, avocado oil unsaponifiable substances and the like), water quality film repairing factors (e.g. caprylic acid/capric triglyceride), anti-irritation components (e.g. turmeric extract, alpha-bisabolol and the like), preservative, antioxidant and the like can be added when the infant skin care oil is prepared into commodities.
Example 1 ]
A nut oil-containing infant skin care oil comprising the following formulation components:
100 parts of compound nut oil, 3 parts of momordica grosvenori volatile oil and 8 parts of vitamin E; wherein, the mass ratio of the compound nut oil is 1:0.2, uniformly mixing sweet almond oil and walnut oil; the sweet almond oil and the walnut oil are skin care base oil well known to those skilled in the art, the sweet almond oil, the walnut oil and the vitamin E are purchased in the market, and the momordica grosvenori volatile oil is prepared by a conventional steam distillation method;
skin care oil preparation: weighing the compound nut oil, the momordica grosvenori volatile oil and the vitamin E according to the proportion, and uniformly stirring at 25 ℃ to obtain the skin care oil.
Example 2 ]
A nut oil-containing infant skin care oil comprising the following formulation components:
100 parts of compound nut oil, 1 part of momordica grosvenori volatile oil and 3 parts of vitamin E; wherein, the mass ratio of the compound nut oil is 1:0.1, uniformly mixing sweet almond oil and walnut oil;
the preparation method of the momordica grosvenori volatile oil comprises the following steps: placing crushed fructus Siraitiae Grosvenorii in deionized water, stirring uniformly, refluxing at 100deg.C for 5 hr to obtain oil-water mixture, extracting the oil-water mixture with n-hexane, collecting n-hexane extract layer, and steaming to remove n-hexane to obtain fructus Siraitiae Grosvenorii volatile oil; wherein, the mass ratio of the crushed fructus momordicae to the deionized water is 1:6, preparing a base material;
skin care oil preparation: weighing the compound nut oil, the momordica grosvenori volatile oil and the vitamin E according to the proportion, and uniformly stirring at 25 ℃ to obtain the skin care oil.
Example 3 ]
A nut oil-containing infant skin care oil comprising the following formulation components:
100 parts of compound nut oil, 3 parts of momordica grosvenori volatile oil, 8 parts of vitamin E and 10 parts of bletilla striata treated matter; wherein, the mass ratio of the compound nut oil is 1:0.2, uniformly mixing sweet almond oil and walnut oil;
the preparation method of the momordica grosvenori volatile oil comprises the following steps: placing crushed fructus Siraitiae Grosvenorii in deionized water, stirring uniformly, refluxing at 100deg.C for 6 hr to obtain oil-water mixture, extracting the oil-water mixture with n-hexane, collecting n-hexane extract layer, and steaming to remove n-hexane to obtain fructus Siraitiae Grosvenorii volatile oil; wherein, the mass ratio of the crushed fructus momordicae to the deionized water is 1:6, preparing a base material;
the preparation of the bletilla striata treatment substance comprises the following steps: weighing crushed bletilla striata, placing into white oil special for cosmetics, slowly stirring at 45 ℃ for 24 hours, filtering, collecting filtrate, and cooling to room temperature to obtain the bletilla striata treated matter; wherein, the mass ratio of the bletilla striata to the white oil is 1:10.
skin care oil preparation: weighing the compound nut oil, the momordica grosvenori volatile oil, the vitamin E and the bletilla striata treated matter according to the proportion, and uniformly stirring at 25 ℃ to obtain the skin care oil.
Example 4 ]
A nut oil-containing infant skin care oil comprising the following formulation components:
100 parts of compound nut oil, 3 parts of momordica grosvenori volatile oil, 8 parts of vitamin E and 5 parts of bletilla striata treated matter; wherein, the mass ratio of the compound nut oil is 1:0.2, uniformly mixing sweet almond oil and walnut oil;
the preparation method of the momordica grosvenori volatile oil comprises the following steps: placing crushed fructus momordicae in 0.2M phosphoric acid buffer solution, heating at 50 ℃ for 15min, placing in an ultrasonic environment with ultrasonic frequency of 40Hz for ultrasonic treatment for 15min to obtain treatment liquid, adding deionized water into the treatment liquid, placing at 100 ℃ for reflux for 6h to obtain oil-water mixed solution, extracting the oil-water mixed solution by using n-hexane, taking an n-hexane extraction layer, and removing n-hexane by rotary evaporation to obtain the fructus momordicae volatile oil; wherein, the mass ratio of the crushed fructus momordicae to the phosphate buffer solution is 1-2.5, and the mass ratio of the crushed fructus momordicae to the deionized water is 1:6, preparing a base material;
the preparation of the bletilla striata treatment substance comprises the following steps: weighing crushed bletilla striata, placing into white oil special for cosmetics, slowly stirring at 40 ℃ for 12 hours, filtering, collecting filtrate, and cooling to room temperature to obtain the bletilla striata treated matter; wherein, the mass ratio of the bletilla striata to the white oil is 1:5.
skin care oil preparation: weighing the compound nut oil, the momordica grosvenori volatile oil, the vitamin E and the bletilla striata treated matter according to the proportion, and uniformly stirring at 25 ℃ to obtain the skin care oil.
Comparative example 1 ]
An infant skin care oil containing nut oil was the same as in example 2 except that no Momordica grosvenori essential oil was added in comparative example 1.
Skin care oil preparation: weighing the compound nut oil and the vitamin E according to the proportion, and uniformly stirring at 25 ℃ to obtain the skin care oil.
Comparative example 2 ]
An infant skin care oil containing nut oil is the same as in example 2, except that the momordica grosvenori volatile oil in example 2 is replaced by momordica grosvenori ethanol extract, and extraction is performed in a conventional extraction mode, wherein the ethanol concentration is 70%, and the feed-to-liquid ratio is 1:15, reflux extraction time is 4h, and extraction temperature is 70 ℃.
< acute skin irritation test >
An acute skin irritation test was performed with reference to cosmetic safety Specification (2021), specifically as follows:
5 SD rats were selected from the male and female rats, and the hairs on both sides of the back spine were cut off 24. 24h before the test, and the skin was not damaged, and the left and right rats were approximately 2cm. Times.2 cm. 0.5ml of the test object is directly smeared on one side of skin, then covered by two layers of gauze, and then fixed by using non-irritating adhesive tape. The other side of the skin is a blank. The application time was 4 hours, after which the residual test object was removed with warm water. At 2 hours after removal of the test subjects, the skin of the back-smeared part of SD rats was observed for erythema and edema, and the degree of erythema and edema was scored according to tables 1 and 2, and the average value of the scores of the 10 groups of tests (i.e., the reaction average score) was taken as the final score. The skin irritation was graded by the test object in table 3. Wherein, the mean response score = (total erythema plus total edema) per total animals.
TABLE 1 erythema degree scoring criteria
TABLE 2 edema degree scoring criteria
TABLE 3 skin irritation intensity evaluation criteria
Sample: the skin care oils of examples 1 to 4 and comparative examples 1 to 2 were used as test substances, and an acute skin irritation test was performed to record the skin irritation intensity, and the specific results are shown in Table 4.
Table 4 results of acute skin irritation test for each of examples and comparative examples
As can be seen from Table 4, the skin care oils prepared in examples 1 to 4 and comparative examples 1 to 2 are all non-irritating to the skin of the rats tested, indicating that the skin care oils prepared in the application are relatively safe.
< stability test >
The skin care oil prepared in examples 1 to 4 and comparative example 1 was placed in an oven at 45 ℃ for 30 days, taken out, compared with a sample placed at room temperature, observed for color and smelled, and the results are shown in table 5:
TABLE 5 results of stability test of skin care oils in examples and comparative examples
As can be seen from Table 5, the skin care oils prepared in examples 1 to 4 and comparative examples 1 to 2 have good stability.
< replication of rat model of chronic eczema >
1. Experimental reagent
2, 4-Dinitrochlorobenzene (DNCB) (analytically pure), acetone solution (analytically pure), depilatory cream (from Midating), baby emollient oil (from Qiangsheng)
2. Experimental animals and groups
90 healthy SD male rats, 200+ -20 g in body weight; after feeding the SD rats with the basic feed and purified water for one week, the rats were randomly divided into blank group, chronic eczema group, example 1 group, example 2 group, example 3 group, example 4 group, comparative example 1 group, comparative example 2 group and model group, and 10 rats were fed in separate cages.
3. Replication of animal models
The skin on the back of the rat was dehaired by using the dehaired paste, the dehaired area was 4cm×4cm, and the dehaired area was stimulated by applying 100 μl of 5% DNCB acetone solution to the skin, once every 5d, and continuously for 4 times. Wherein, the blank group is not excited at all, and the rest experimental groups are excited. The skin at the dehaired sites after the first challenge was gradually developed as erythema, papules, scratches, scales, crusting, after each challenge the skin lesions were recorded and scored against table 6, taking the average score of 10 groups of experiments, taking the average of four challenge averages as the final skin lesions score, and the specific results are shown in table 7.
4. Molding result
The back skin of the rat is repeatedly stimulated by using 2, 4-dinitrochlorobenzene, so that the back skin of the rat has skin damage manifestations such as erythema, pimple, scale, moss and the like which accord with clinical manifestations of chronic eczema, and a chronic eczema rat model is established. The results show that: the back skin of the rats in the blank group is asymptomatic, is light red, and has clear skin texture and soft texture; more specifically, as shown in table 7, the backs of the rats in the example 1 group to the example 4 group and the comparative example 1 group to the comparative example 2 group all had obvious skin lesions, and had skin symptoms such as visible erythema, scales, crusting, rough thickening, pigmentation and the like, which met the diagnosis criteria of chronic eczema, and the rat modeling was successful.
TABLE 6 skin lesion manifestation scoring criteria
| Manifestations of skin lesions | Score value |
| No skin damage (asymptomatic) | 0 |
| Slight skin lesions (symptoms only visible after careful observation) | 0~1 |
| Moderate skin damage (symptoms can be seen immediately) | 1~2 |
| Severe skin damage (symptoms are very obvious) | 2~3 |
TABLE 7 skin loss scoring results for each experimental group
| Experimental group | Skin damage scoring |
| Blank space | 0 |
| Chronic eczema group | 2.813 |
| Example 1 | 2.790 |
| Example 2 | 2.788 |
| Example 3 | 2.775 |
| Example 4 | 2.800 |
| Comparative example 1 | 2.810 |
| Comparative example 2 | 2.820 |
| Model | 2.805 |
5. Therapeutic effects
In each experimental group, the blank group and the chronic eczema group are not treated by medicines, the skin care oil prepared in the examples 1-4 is applied in the examples 1-4, the skin care oil prepared in the comparative examples 1-2 is applied in the comparative examples 1-2, the skin care oil is applied in the model group, the skin care oil is applied to skin of a newborn infant, the skin care oil is applied in an amount of 0.1mL per square centimeter of skin, the skin care oil is applied twice daily for 12 hours each time, the application is continuous for 7 days, no water is forbidden after the last application, the skin damage of the affected part of each group of rats is scored after 12 hours, and the experimental results are shown in Table 8.
Table 8 results of scoring skin lesions on the back of rats for each experimental group
| Experimental group | Skin damage scoring |
| Blank space | 0 |
| Chronic eczema group | 2.860 |
| Example 1 | 0.52 |
| Example 2 | 0.50 |
| Implementation of the embodimentsExample 3 | 0.30 |
| Example 4 | 0.29 |
| Comparative example 1 | 2.04 |
| Comparative example 2 | 1.61 |
| Model | 2.13 |
As can be seen from table 8, the skin care oil prepared by the present application can effectively relieve chronic eczema symptoms of rats by applying the skin care oil, wherein the data of examples 3 to 4 and examples 1 to 2 show that the addition of the bletilla striata treatment substance can further treat chronic eczema of rats.
< Effect of paraxylene-induced mouse auricle swelling >
30 mice (18-22 g) were selected and each half was randomly divided into a blank group, a group of example 2 and a group of comparative example 1 according to gender and weight, and 10 mice each. The right ears of the mice in the blank group are uniformly coated with 0.5ml of physiological saline, the right ears of the mice in the embodiment 2 group are uniformly coated with 0.5ml of skin care oil in the embodiment 2, the right ears of the mice in the comparison 1 group are uniformly coated with 0.5ml of skin care oil in the comparison 1 group, and after 10 minutes of coating, each right ear of each group of mice is uniformly coated with 0.05ml of dimethylbenzene inflammation, and the left ear of each right ear of each group is not coated with the skin care oil in the comparison. After 30 minutes of xylene application, mice were sacrificed, left and right ears of the mice were cleaned with distilled water and the water was wiped dry, the same area of the left and right ears was cut off with a punch with a diameter of 8mm, and the mice were weighed with an electronic balance. The swelling degree of each experimental group was calculated using the difference between the left and right ear weights as a swelling degree index, and the specific results are shown in table 9.
Table 9 results of swelling degree and swelling inhibition ratio of each experimental group
| Experimental group | Number of mice | Swelling degree (mg) |
| Blank space | 10 | 9.2 |
| Example 2 | 10 | 3.8 |
| Comparative example 1 | 10 | 6.3 |
As can be seen from table 9, the swelling degree of mice is effectively relieved by applying the skin care oil of the present application, which shows that the skin care oil of the present application has a certain anti-inflammatory effect.
In conclusion, the skin care oil prepared from the momordica grosvenori volatile oil has certain effects of relieving chronic eczema and diminishing inflammation, and the symptoms of the chronic eczema can be further relieved after the bletilla striata treatment substance is added, and the skin care oil is relatively safe to use due to the fact that all components are derived from natural components.
Although embodiments of the present application have been disclosed above, it is not limited to the details and embodiments shown, it is well suited to various fields of use for which the application is suited, and further modifications may be readily made by one skilled in the art, and the application is therefore not to be limited to the particular details and examples shown and described herein, without departing from the general concepts defined by the claims and the equivalents thereof.
Claims (1)
1. The infant skin care oil containing the nut oil is characterized by comprising the following components in parts by weight:
100 parts of compound nut oil, 1-3 parts of momordica grosvenori volatile oil, 3-8 parts of vitamin E and 5-10 parts of bletilla striata treated matter; wherein, the mass ratio of the compound nut oil is 1: 0.1-0.2 of sweet almond oil and walnut oil are uniformly mixed to prepare the sweet almond oil;
the preparation method of the momordica grosvenori volatile oil comprises the following steps: placing crushed fructus momordicae in deionized water, uniformly stirring, placing at 100 ℃ and refluxing for 5-6 hours to obtain an oil-water mixed solution, and extracting and rotary steaming the oil-water mixed solution to obtain the fructus momordicae volatile oil; before placing crushed fructus momordicae in deionized water, placing the crushed fructus momordicae in 0.2M phosphoric acid buffer solution, then placing the fructus momordicae in 45-50 ℃ for heating for 15-20 min, then placing the fructus momordicae in an ultrasonic environment for ultrasonic treatment for 15-20 min to obtain treatment solution, and then adding deionized water into the treatment solution for reflux; the mass ratio of the crushed fructus momordicae to the phosphate buffer solution is 1:2.5;
the preparation of the bletilla striata treatment substance comprises the following steps: weighing crushed bletilla striata, placing the crushed bletilla striata into white oil special for cosmetics, slowly stirring the mixture at 40-45 ℃ for 12-24 hours, filtering the mixture, and collecting filtrate and cooling the filtrate to room temperature to obtain the bletilla striata treated matter; wherein, the mass ratio of the bletilla striata to the white oil is 1: 5-10;
the preparation of the skin care oil specifically comprises the following steps: and weighing the compound nut oil, the momordica grosvenori volatile oil, the vitamin E and the bletilla striata treated matter according to the proportion, and uniformly stirring at 20-25 ℃ to obtain the compound nut oil.
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