CN111184653A - Vegetable oil skin care composition and preparation method thereof - Google Patents

Abstract

The invention relates to the field of cosmetics, in particular to a vegetable oil skin care composition and a preparation method thereof. The composite material consists of the following raw materials in parts by weight: 40-80 parts of camellia oil, 5-15 parts of jojoba oil, 0.5-5 parts of white pond flower seed oil, 0.01-0.5 part of tocopherol, 0.2-5 parts of linseed oil and 10-35 parts of olive squalane; the preparation method comprises the following steps: uniformly mixing jojoba oil, chinaroot greenbrier seed oil, tocopherol, linseed oil and olive squalane to obtain a pre-solution; and adding camellia oil into the pre-liquid, uniformly mixing, filtering and discharging to obtain the vegetable oil skin care composition. The invention adopts various natural vegetable oils to effectively reduce the irritation to human body; the obtained vegetable oil skin care composition can effectively repair the horny layer of the skin and has good anti-inflammatory effect.

Description

Vegetable oil skin care composition and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, in particular to a vegetable oil skin care composition and a preparation method thereof.

Background

The skin is the largest organ of the human body and is composed of the epidermal layer, the dermal layer and the subcutaneous adipose tissue. An intact human skin surface is of vital importance to the human body, as the skin is able to maintain fluid balance, regulate body temperature, and protect the human body. The stratum corneum is the outermost part of the epidermis and is composed of mainly 10 to 20 flat, dead cells without nuclei. When these cells are shed, the cells in the underlying stratum basale are pushed up to form a new stratum corneum. The stratum corneum serves primarily to protect its underlying tissues from infection, dehydration, and stress from chemical and external forces. The cells of the stratum corneum contain keratin, which helps to reduce water evaporation and even to absorb water, keeping the skin moist. The skin of many animals, including humans, can wrinkle after being soaked in water for a period of time due to the water absorption of keratin. The stratum corneum typically varies from 10 to 40 microns depending on how much protection is required for its corresponding body part.

After the cuticle falls off automatically or artificially, the cuticle layer can rapidly proliferate keratinocytes and transform the keratinocytes into dead anucleated keratinocytes to be transported to the skin surface, and the total process takes about 14 days. Excessive removal of the stratum corneum results in very thin stratum corneum, sensitive skin and severe red streaks. The resulting red, swollen, hot, painful, itchy skin may cause inflammatory reactions in the skin. Meanwhile, with the serious environmental pollution and the increase of daily activities such as irregular life, poor sleep, dyspepsia and the like under high-rhythm work, the generation of skin inflammatory reaction is gradually increased.

At present, most of skin repair modes realized by external products are hormone medicines, but different degrees of skin atrophy, thinning, telangiectasis, skin flushing or generation of stasis points and plaques, dry and black skin or ichthyosis-like change, alopecia, hirsutism, hormone acne and the like can occur on parts using the medicines for a long time. Such products are therefore not recommended as long-term skin repair products for daily use.

In addition to pharmaceutical products, there are many skin repair products listed in the cosmetic category in the commercial products and in the publicly issued literature and patents. The active ingredients are mostly ceramides which are the main ingredients of intercellular lipids, or some fruit/plant extracts having cell repairing effect.

The Chinese patent office discloses an invention patent application (CN110179711A) of essence with the function of repairing skin barrier in 2019, 08 and 30, and the components of the essence comprise hexapeptide-11, SCLEROTIUM rolfsii (SCLEROTIUM rolfsii) gum, cyclopentadimethylsiloxane, silver, ceramide-3, plant extracts, polyhydric alcohol, a surfactant, a humectant and water. The principle is to accelerate skin repair by supplementing ceramide, a main component of intercellular lipid. However, as a component of daily skin care products, ceramides impose high demands on the production process of cosmetics. It has high dissolving temperature, easy crystallization and no stability in the recipe. Secondly, the price cost is high, and the ingredient is difficult to be included in the skin care product with the same price.

The patent application (CN109700747A) of the Chinese patent office in 2019, 05 and 03 discloses a skin care composition with a skin repair function, and the main functional components of the skin care composition are pseudo-ginseng powder, an angelica dahurica extract, a bletilla striata extract, pearl powder and water-soluble chitosan. But are limited by the production volume and cannot be used in large quantities in daily cosmetics.

Currently, there are commercially available products for skin care of infants which use camellia oil as the sole raw material. It is used for the buttocks of infant, and has effects of preventing skin chapping and redness. However, the use of a single pure plant-derived oil as the sole raw material for a commercial product poses risks, including product stability problems associated with a single plant-derived raw material, regional and seasonal variation problems with trace elements, and the like. In addition, the efficacy of a single raw material is more single than that of a compound composition.

The patent office of China (CN110100909A) in 2019, 08 and 09, discloses a preparation method of a compound oily composition with broad-spectrum antibacterial effect and remarkable antibacterial effect and an application of the compound oily composition (CN110100909A), wherein safflower seed oil, peanut oil, linseed oil, walnut oil, sunflower seed oil, camellia oil and six substances are compounded, and an effective antibacterial ingredient chlorhexidine acetate is added to inhibit various pathogenic bacteria through synergistic action. The main bacteriostatic component in the patent is chlorhexidine acetate, and the maximum mass percentage content of 6 compounded oils is safflower seed oil (80-81%) and peanut oil (9-10%). The content of other oil and fat is not more than 5%.

The Chinese patent office in 2017, 04 and 26 discloses an invention patent application (CN106580762A) of a skin care product composition with wrinkle smoothing and resisting effects, wherein raw materials of the composition protected by the patent comprise 2-5% of a plant source emulsifier, and one or more selected from jojoba oil, sweet almond oil, olive oil, camellia oleosa seed oil, wheat germ oil, safflower seed oil, grape seed oil or white pond flower seed oil. But only as an emulsifier and not as a primary efficacy active ingredient.

The chinese patent office in 2018, 01, 19 discloses an invention patent application (CN107595751A) of a lip skin care composition, which mainly comprises a dust scented honey, a polar solvent, an emulsifier, a thickener, and an emollient. The emollient is selected from oil Camellia seed oil, meadowfoam seed oil, oleum Lini, jojoba seed oil, and at least one of other optional components. The above plant-extracted oils and fats in this patent are used only as emollients, not as main efficacy active ingredients.

Disclosure of Invention

The invention provides a vegetable oil skin care composition and a preparation method thereof, aiming at solving the problems that the existing commercially available skin care composition for repairing and protecting skin is difficult to prepare and high in cost, or limited in raw material yield and difficult to produce on a large scale, and a skin care product using a common and easily-obtained pure natural vegetable oil extract as a main functional active ingredient is not available. The invention aims to: firstly, screening specific high-quality vegetable oil with large yield as a main effective active ingredient through compounding research, and reducing irritation to a human body; secondly, compounding by adopting a mode of combining various vegetable oils, the raw materials are wide in source, and the influence caused by regional and seasonal differences is reduced; thirdly, the obtained composition can repair the stratum corneum of the skin; and fourthly, the use feeling of the skin care composition is improved, so that the skin care composition is smoother.

In order to achieve the purpose, the invention adopts the following technical scheme.

A vegetable oil skin care composition comprises vegetable oil and water,

the vegetable oil skin care composition comprises the following raw materials:

camellia oil, jojoba oil, chinlon oil, tocopherol, linseed oil and olive squalane.

The raw materials are common components in the used raw material catalog of cosmetics, have high safety and wide sources, and the preparation and extraction processes of the raw materials are mature. The ingredients are described below.

Camellia oil, also known as tea oil, camellia seed oil and the like, is composed of components such as fatty acid, camellin, phospholipid, saponin, vitamin E, tannin and the like, and generally, the tea oil is rich in unsaturated fatty acid, the fatty acid composition of the tea oil is very similar to that of olive oil, the camellia oil also has good skin care and skin moistening effects, triglyceride components in a skin lipid membrane can be effectively supplemented, the camellia oil not only can supplement components of the skin lipid membrane of a human body, but also has a promoting effect on percutaneous absorption of medicines, and therefore, the camellia oil has a good using effect when being used as base oil.

The jojoba oil is a waxy liquid product, has components very similar to natural oil produced by human skin, has natural moisturizing and repairing effects, is suitable for various skins including sensitive skins such as psoriasis, eczema and the like, does not generate irritation to the skins, and has very excellent affinity to the skin surfaces of human bodies.

The white pond flower seed oil is known as the most stable vegetable oil, has good oxidation resistance, can effectively improve the preservation and storage capacity of the whole vegetable oil skin care composition, can also achieve the purpose of resisting skin oxidation aging and the like during use, has good use effect, and can enrich the texture of the composition in the composition and provide excellent skin care performance.

The tocopherol is a hydrolysate of vitamin E (ve), is a natural oil-soluble antioxidant which is produced in large quantity at present, can play a very excellent antioxidant effect when being compounded with the meadowfoam seed oil for use, and the generated synergistic effect is more favorable for accelerating the repair effect of damaged skin.

The linseed oil contains high content of unsaturated fatty acid, especially contains more α -linolenic acid, and also contains Ve which is a strong and effective free radical scavenger, has the effects of delaying senility and resisting oxidation, enables the skin to be younger, more moist, softer and elastic, enables the skin to breathe and sweat normally, and solves or relieves various skin problems.

The olive squalane is derived from olive oil, is a natural skin-moistening oil, and has good oxidation resistance and thermal stability. Squalane is a lipid close to sebum of a human body, has strong affinity, can be integrated with the skin lipid membrane of human body, forms a natural barrier on the surface of the skin, can inhibit the peroxidation of skin lipid, can effectively permeate into the skin, promotes the proliferation of skin basal cells, and has obvious physiological effects of delaying skin aging, improving and eliminating chloasma.

As a preference, the first and second liquid crystal compositions are,

the vegetable oil skin care composition is prepared from the following raw materials in parts by weight:

40-80 parts of camellia oil, 5-15 parts of jojoba oil, 0.5-5 parts of chinlon oil, 0.01-0.5 part of tocopherol, 0.2-5 parts of linseed oil and 10-35 parts of olive squalane.

By adopting the proportion, various raw materials are reasonably combined, and the components can generate a better synergistic matching effect. Particularly, a plurality of vegetable oils are adopted to be matched for use, under the condition that the single camellia oil is adopted, the performance of the product is easy to generate larger fluctuation due to the difference of raw material sources, and the product can generate larger greasy feeling when in use, and the greasy feeling can be effectively reduced after the plurality of vegetable oils are compounded.

As a preference, the first and second liquid crystal compositions are,

the content of unsaturated fatty acid in the camellia oil is more than or equal to 85 percent.

The camellia oil with higher unsaturated fatty acid content can further improve the skin moistening and caring effects.

As a preference, the first and second liquid crystal compositions are,

the long-chain fatty acid content of the white Potentilla seed oil is more than or equal to 95%.

The long-chain fatty acid can effectively improve the oxidation resistance of the meadowfoam seed oil.

A method for preparing a vegetable oil skin care composition,

the preparation method comprises the following steps:

1) uniformly mixing jojoba oil, chinaroot greenbrier seed oil, tocopherol, linseed oil and olive squalane to obtain a pre-solution;

2) and adding camellia oil into the pre-liquid, uniformly mixing, filtering and discharging to obtain the vegetable oil skin care composition.

The preparation method is simple and efficient, can be used for rapid and large-scale preparation and production, and the camellia oil is used as a solvent phase, is firstly uniformly mixed with all the components, and then is added into the camellia oil, thereby being beneficial to improving the uniformity of all the components.

As a preference, the first and second liquid crystal compositions are,

heating the mixture in the step 1) to 40-55 ℃.

The preheating during mixing can effectively improve the mixing efficiency of each raw material.

As a preference, the first and second liquid crystal compositions are,

and 2) after uniformly mixing, naturally cooling to a temperature of less than or equal to 35 ℃.

After being mixed uniformly, the mixture is cooled down, so that insoluble impurities generated by the reaction among various components can be effectively separated out and removed.

The invention has the beneficial effects that:

1) various natural vegetable oils are adopted to replace the traditional artificial synthetic products, so that the components are more natural;

2) the used various vegetable oils have wide sources, and the influence of product performance fluctuation caused by regional and seasonal differences can be reduced, so that the product performance is more stable;

3) the obtained vegetable oil skin care composition can effectively repair the horny layer of the skin;

4) the obtained vegetable oil skin care composition has good use feeling, and is smooth and non-greasy;

drawings

FIG. 1 is a microscopic examination of a mouse skin section with stratum corneum repaired in test I;

FIG. 2 is a microscopic examination of skin sections of mice with unrepaired stratum corneum exfoliation in test I;

FIG. 3 is a microscopic photograph of a skin section of a normal mouse in test I;

FIG. 4 is a microscopic examination of a section of mouse skin at an initial state after exfoliation of the stratum corneum in test I.

Detailed Description

The invention is described in further detail below with reference to specific embodiments and the attached drawing figures. Those skilled in the art will be able to implement the invention based on these teachings. Moreover, the embodiments of the present invention described in the following description are generally only some embodiments of the present invention, and not all embodiments. Therefore, all other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.

Unless otherwise specified, the raw materials used in the examples of the present invention are all commercially available or available to those skilled in the art; unless otherwise specified, the methods used in the examples of the present invention are all those known to those skilled in the art. Unless otherwise specified, the percentages (%) in the examples of the present invention are all mass percentages (wt%).

If not specifically stated, the content of unsaturated fatty acids in the camellia oil used in the embodiment of the invention is 85-95%; the long-chain fatty acid content of the white chinaroot herb seed oil used in the embodiment of the invention is 97-98%.

Example 1

The formulation is shown in table 1:

table 1: example 1 formulation

Camellia oil	70％
		Jojoba oil	10％
Potentilla chinensis seed oil	2％
		Tocopherol	0.1％
Olive squalane	12.9％
		Linseed oil	5％

The preparation method comprises the following steps: sequentially adding olive squalane, jojoba oil, white chinlon oil, linseed oil and tocopherol into a reaction kettle, starting stirring, heating to 45 ℃, stirring until the mixture is fully mixed to obtain a pre-liquid, adding the pre-liquid into camellia oil, stirring for 20min to make the pre-liquid uniform, naturally cooling to below 35 ℃, and filtering to remove solid impurities to obtain the vegetable oil skin care composition.

Example 2

The formulation is shown in Table 2:

table 2: example 2 formulation

Camellia oil	80％
		Jojoba oil	5％
Potentilla chinensis seed oil	0.5％
		Tocopherol	0.1％
Olive squalane	11.4％
		Linseed oil	3％

The preparation method is the same as that of example 1.

Example 3

The formulation is shown in Table 3:

table 3: example 3 formulation

Camellia oil	60％
		Jojoba oil	15％
Potentilla chinensis seed oil	0.5％
		Tocopherol	0.1％
Olive squalane	20％
		Linseed oil	4.4％

The preparation method is the same as that of example 1.

Example 4

The formulation is shown in Table 4:

table 4: example 4 formulation

Phase a was prepared as in example 1, and phase B was prepared as follows: dispersing carbomer in partial deionized water to prepare 2% dispersion for later use; sodium dehydroacetate was dispersed in a portion of deionized water to make a 10% solution for use. Heating the rest deionized water to 65-70 deg.C, adding glycerol and polyglycerol-6 stearate, stirring to dissolve completely, adding dispersed carbomer, averaging for 10 min, adding sodium dehydroacetate water solution, and stirring while maintaining the temperature at 60-70 deg.C to obtain water phase. Adding oleum Camelliae Japonicae into the water phase, stirring for 10-20 min, maintaining the temperature at 53-55 deg.C, opening vacuum to-0.04 MPa, and opening the average value for 10 min. And finally, stirring, defoaming and simultaneously cooling, and when the temperature of the material body is reduced to below 35 ℃, discharging the material body until bubbles of the material body are completely removed, thus obtaining a phase B. Mixing the phase A and the phase B with stirring.

Example 5

The formulation is shown in Table 5:

table 5: example 5 formulation

Camellia oil	40％
		Jojoba oil	15％
Potentilla chinensis seed oil	5％
		Tocopherol	0.5％
Olive squalane	35％
		Linseed oil	4.5％

The preparation method is the same as that of example 1.

Example 6

The formulation is shown in Table 6:

table 6: example 6 formulation

Camellia oil	70％
		Jojoba oil	15％
Potentilla chinensis seed oil	4.79％
		Tocopherol	0.01％
Olive squalane	10％
		Linseed oil	0.2％

The preparation method is the same as that of example 1.

Example 7

The formulation is the same as example 1, and the preparation method comprises the following steps: sequentially adding olive squalane, jojoba oil, white chinlon oil, linseed oil and tocopherol into a reaction kettle, starting stirring, heating to 40 ℃, stirring until the mixture is fully mixed to obtain a pre-liquid, adding the pre-liquid into camellia oil, stirring for 20min to be uniform, cooling to 35 ℃, and filtering to remove solid impurities to obtain the vegetable oil skin care composition.

Example 8

The preparation method comprises the following steps: sequentially adding olive squalane, jojoba oil, white chinlon oil, linseed oil and tocopherol into a reaction kettle, starting stirring, heating to 55 ℃, stirring until the mixture is fully mixed to obtain a pre-liquid, adding the pre-liquid into camellia oil, stirring for 20min to be uniform, cooling to 35 ℃, and filtering to remove solid impurities to obtain the vegetable oil skin care composition.

Comparative example 1

The camellia oil in the example 1 is replaced by the silicone oil, and the rest components, the content of each component and the preparation method are unchanged.

Comparative example 2

Pure camellia oil was used as comparative example 2.

Comparative example 3

The formulation is shown in Table 7:

table 7: comparative example 3 formulation

The preparation method comprises the following steps: dispersing carbomer in partial deionized water to prepare 2% dispersion for later use; dispersing sodium dehydroacetate in partial deionized water to prepare a 10% solution for later use, heating the rest deionized water to 65-70 ℃, adding glycerol and polyglycerol-6 stearate, stirring until the materials are completely dissolved, adding dispersed carbomer, averaging for 10 minutes, adding a sodium dehydroacetate aqueous solution, continuously stirring, keeping the temperature at 60-70 ℃ as a water phase, adding camellia oil into the water phase, continuously stirring for 10-20 minutes, keeping the temperature at 53-55 ℃, starting vacuum, adjusting the pressure to be about-0.04 MPa, starting averaging for 10 minutes, finally stirring, defoaming, simultaneously cooling, cooling when the temperature of a material body is reduced to below 35 ℃, and completely removing bubbles, and discharging.

Comparative example 4

The formulation is shown in Table 8:

table 8: comparative example 4 formulation

Camellia oil	10％
		Jojoba oil	10％
Potentilla chinensis seed oil	45％
		Tocopherol	0.1％
Olive squalane	29.9％
		Linseed oil	5％

The preparation method is the same as that of example 1.

Comparative example 5

The formulation is shown in Table 9:

table 9: comparative example 5 formulation

The preparation method is the same as that of example 1.

Comparative example 6

The formulation is shown in Table 10:

table 10: comparative example 6 formulation

Camellia oil	60％
		Jojoba oil	15％
Potentilla chinensis seed oil	0％
		Tocopherol	0.5％
Olive squalane	20％
		Linseed oil	4.5％

The preparation method is the same as that of example 1.

Comparative example 7

The formulation is shown in Table 11:

table 11: comparative example 7 formulation

Camellia oil	80％
		Jojoba oil	15％
Potentilla chinensis seed oil	0.5％
		Tocopherol	0.1％
Olive squalane	0％
		Linseed oil	4.4％

The preparation method is the same as that of example 1.

The detection tests were conducted on the examples 1 to 8 and comparative examples 1 to 7 thus obtained. The detection test comprises the following aspects:

test I: test for repairing function of skin cuticle of mouse

Selecting 6 (8-10 weeks) male nude mice, dividing into three groups A and B, firstly, continuously sticking and rapidly tearing off the skin 3cm x 3cm test parts on the left side and the right side of the nude mice by using an adhesive tape for a plurality of times until the skin has a small blood spot, and stopping sticking and tearing. The test parts on the left and right sides of the group A mice are respectively coated with the test example 3 of the invention, the test parts on the left and right sides of the group B mice are coated with the test example 1 of the invention, the test parts are coated once a day, after four days of continuous coating, the animals are killed, the skin tissues of the test parts are dissected, and pathological section microscopic examination is carried out for photographing.

Wherein, the example 3 is a pure plant skin care composition which meets the component proportion and the process requirement provided by the patent, the comparative example 1 is a composition which does not contain camellia oil, and other components are the same as the example 3 and are used as a control group.

Another 3 (8-10 weeks) male nude mice are taken, one side of each nude mouse is continuously pasted and quickly torn off by an adhesive tape for a plurality of times until the skin has a small blood spot, the sticking and tearing are stopped, the nude mice are used as a model group, the other side of each nude mouse is not processed and used as a normal group, the animals are killed, the skin tissues of the test part are dissected, and the skin tissues are taken for pathological section microscopic examination and photographing.

The section microscopic examination images are shown in fig. 1 to 4, wherein fig. 1 is a section microscopic examination image of the skin of a group A mouse, fig. 2 is a section microscopic examination image of the skin of a group B mouse, fig. 3 is a section microscopic examination image of the skin of a normal group mouse, and fig. 4 is a section microscopic examination image of the skin of a model group mouse. It is obvious from the figure that after the adhesive tape is continuously adhered and quickly torn off for a plurality of times, the figure 4 shows that the horny layer on the surface of the skin of the mouse falls off obviously, and if the horny layer cannot be repaired in time, inflammation is easy to generate, as shown in figure 2, the horny layer is not repaired after the product in the comparative example 1 is smeared for four days, but after the vegetable oil skin-care composition prepared in the example 3 is used, the horny layer is repaired, which shows that the vegetable oil skin-care composition plays a role in repairing the horny layer, and the thickness of the restored horny layer is basically equivalent to that of the normal group shown in figure 3, thus the repairing effect is good and effective.

An equivalent test was also further conducted using comparative example 5 and comparative example 6. After the test, the cuticle which is peeled off after being smeared for four days is repaired to a certain degree, but the cuticle is thinner and the repairing effect is more general, and the cuticle is basically repaired to the more normal thickness as shown in fig. 1 and fig. 3 after being smeared for nine days (comparative example 5) and eleven days (comparative example 6). The compound of the common coltsfoot herb seed oil and the tocopherol can effectively promote the repair of the cuticle and accelerate the repair speed, and the single use effect is poor.

Test II: anti-inflammatory test for inflammatory factors secreted by SDS-induced 3D skin model A whole human skin model purchased from Seanpun was maintained in a sterile environment for a period of time, and the skin model was removed, freed of residual agarose gel medium, and transferred to a 12-well plate previously supplemented with maintenance medium at 37 ℃ with 5% CO₂The culture was carried out for 24 hours.

A0.5% SDS solution, comparative example 1, comparative example 2, comparative example 4, example 1, example 2 was sterilized by filtration through a 0.22 μm filter and was used. The following experimental groups:

blank control group: add 40. mu.l PBS solution

SDS group: mu.l of 0.5% SDS was added to 20. mu.l of PBS solution

Comparative example 1: 20 μ l 0.5% SDS, 20 μ l comparative example 1 composition was added

Comparative example 2: 20 μ l of 0.5% SDS, 20 μ l of the purified camellia oil of comparative example 2 was added

Comparative example 4: 20 μ l 0.5% SDS, 20 μ l comparative example 4 composition was added

Example 1: mu.l of 0.5% SDS, 20. mu.l of the composition of example 1 was added

Example 2: mu.l of 0.5% SDS, 20. mu.l of the composition of example 2 was added

Transferring the 3D skin model in the culture basket to a 12-well plate containing 2ml of detection culture medium, loading the 3D skin model into the skin model basket according to experimental groups, smearing the skin model basket on the surface of the skin model, continuing culturing at 37 ℃ for 24h by 5% CO2, collecting the detection culture medium below the skin model, and detecting the content levels of inflammatory factors IL-1 β and IL-6 according to the operation method of the kit.

The test results are shown in table 12 below:

table 12: anti-inflammation test results for inflammatory factors secreted by SDS-induced 3D skin model

Grouping	1L-1 β content (ng/mL)	1L-6 content (ng/mL)
			Blank control group	20.94±1.16	11.48±1.79
0.5% SDS group	28.80±1.37	31.64±2.07
			Comparative example 1	27.69±1.16	30.68±2.49
Comparative example 2	22.06±2.09	12.09±3.4
			Comparative example 4	22.07±1.97	12.73±2.11
Example 1	17.42±0.08	5.54±1.03
			Example 2	17.87±0.39	6.64±0.20

The above-mentioned experimental results prove that,

the skin model in the blank control group had lower IL-6 and IL-1 β levels, and both IL-6 and IL-1 β levels increased 24h after 0.5% SDS stimulation, whereas examples 1 and 2 had inhibitory effects on IL-1 β and IL-6 and had statistical differences (P <0.05) compared to the SDS group after the intervention of examples 1 and 2;

IL-1 β -6 of the comparative example 1 group was not statistically different (P >0.05) from the SDS group, i.e., comparative example 1 had no inhibitory effect on IL-1 β and IL-6;

the IL-1 β -6 of the comparative examples 2 and 4 had statistical differences (P <0.05) compared to the SDS group, i.e., the comparative examples 2 and 4 also had inhibitory effects on IL-1 β and IL-6, but IL-1 β -6 was significantly higher than IL-1 β -6 of examples 1 and 2, i.e., the comparative examples 2 and 4 had significantly worse inhibitory effects on IL-1 β and IL-6 than those of examples 1 and 2.

Test III: smoothness test on the forearm of the subject

The skin friction test was performed on the forearms of 16 subjects (eight each of men and women) aged 25-36. The instruments used were a multifunctional skin test platform (MPA4) and a skin friction test probe (FR700) from CK, germany. The subject tested the skin friction baseline after 30 minutes between constant temperature and humidity. The test areas of the left and right forearms of the subject were then coated with the product (2 mg/cm) according to the following groups, respectively²)：

Comparative example 3: certain skin cream

Example 3: vegetable oil skin care composition

Example 4: comparative example 3 skin cream was mixed with the skin care composition of example 1 in equal amount

The subjects who had finished applying the product continued sitting still for 1 hour at constant temperature and humidity, and then tested the skin friction of each test area.

The test results are shown in table 13 below:

table 13: results of smoothness tests on the forearm of the subject

Grouping	Skin friction force (AU)
		Basal skin value	100.14±42.12
Example 3	94.01±33.67
		Example 4	139.94±66.10
Comparative example 3	489.25±119.85

The above experimental results show that the skin is clean and has less friction without any skin care product applied, and comparative example 3 alone significantly reduces the skin friction (i.e., improves skin smoothness). The skin friction of example 4 (i.e., comparative example 3 cream mixed with equal mass of the skin care composition of example 1) was not statistically different (P >0.05) from the skin base. This demonstrates that the skin care composition used in combination with comparative example 3 improves its skin feel (skin smoothness). Example 3 is a characteristic that the vegetable oil skin care composition can more effectively maintain the body height smoothness.

And (3) test IV: greasy feeling test

The test is a double-blind test, random passers-by tests are carried out on a large market square in a total of 21 days after three weeks, and each passer-by is respectively coated on the back of the hand by using the products of example 3 and comparative example 7 to carry out greasy feeling tests and fill in a form. The total number of valid data tables is 1131 cases, where: 35 test road persons in the age group of 8-12 years are used, 97 test road persons in the age group of 13-18 years are used, 416 test road persons in the age group of 19-23 years are used, 466 test road persons in the age group of 24-40 years are used, and 117 test road persons in the age group of more than 40 years are used; the total number of male test passers is only about 9.4%, the test result mainly takes females as main results, but the data obtained by the cosmetics of the type mainly take females as main use groups still have high credibility.

The filling-up is mainly used for scoring and judging the greasy feeling, the score is 1-10, and the higher the score is, the more the greasy the filling-up is. All fractions were averaged, one decimal place. The test results are shown in table 14 below:

table 14: evaluation of greasy feeling test

Group of	Mean score
		Example 3	8.9
Comparative example 7	5.5

From the above scoring results, it is apparent that the greasiness of the vegetable oil skin care composition is greatly increased without the addition of olive squalane, and it is mainly known from passersby that there is a feeling of gas impermeability.

Claims (7)

1. A vegetable oil skin care composition characterized in that,

the vegetable oil skin care composition comprises the following raw materials:

camellia oil, jojoba oil, chinlon oil, tocopherol, linseed oil and olive squalane.

2. The vegetable oil skin care composition according to claim 1,

the vegetable oil skin care composition is prepared from the following raw materials in parts by weight:

40-80 parts of camellia oil, 5-15 parts of jojoba oil, 0.5-5 parts of chinlon oil, 0.01-0.5 part of tocopherol, 0.2-5 parts of linseed oil and 10-35 parts of olive squalane.

3. The vegetable oil skin care composition according to claim 1 or 2,

the content of unsaturated fatty acid in the camellia oil is more than or equal to 85 percent.

4. The vegetable oil skin care composition according to claim 1 or 2,

the long-chain fatty acid content of the white Potentilla seed oil is more than or equal to 95%.

5. A method of preparing a vegetable oil skin care composition according to any one of claims 1 to 4,

the preparation method comprises the following steps:

1) uniformly mixing jojoba oil, chinaroot greenbrier seed oil, tocopherol, linseed oil and olive squalane to obtain a pre-solution;

2) and adding camellia oil into the pre-liquid, uniformly mixing, filtering and discharging to obtain the vegetable oil skin care composition.

6. The method of preparing a vegetable oil skin care composition according to claim 5,

heating the mixture in the step 1) to 40-55 ℃.

7. The method of preparing a vegetable oil skin care composition according to claim 5,

and 2) after uniformly mixing, naturally cooling to a temperature of less than or equal to 35 ℃.

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