CN112451463B - Skin care composition for resisting skin photoaging - Google Patents
Skin care composition for resisting skin photoaging Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Abstract
The invention relates to a skin care composition for resisting skin photoaging, which consists of effective components and medically acceptable auxiliary materials, and is characterized in that the effective components consist of pearl aqueous extract and sea grape extract in the following weight percentage: 16-24% of pearl water extract and 76-84% of sea grape extract; the pearl aqueous extract is prepared by the following steps: adding appropriate amount of water into Margarita powder, stirring at 4 deg.C, centrifuging, collecting supernatant, dehydrating, and drying to obtain Margarita water extract; the sea grape extract is prepared by the following method: cleaning Ampelopsis grossedentata to remove salt, squeezing, centrifuging, collecting supernatant, dehydrating, and drying to obtain the Ampelopsis grossedentata extract. The effective components of the skin care composition, namely the pearl aqueous extract and the sea grape extract, have synergistic effect and remarkable effect of resisting skin photoaging.
Description
Technical Field
The invention relates to the field of cosmetics, in particular to a skin care composition containing a raw material with an undetermined structure from marine organisms and resisting photoaging.
Background
Skin aging is classified into intrinsic and extrinsic aging, which is skin aging caused by external environmental factors, mainly caused by ultraviolet radiation, and thus is also called photoaging. With the increasing severity of environmental problems, the ultraviolet rays reaching the ground are gradually increased due to the destruction of the ozone layer, the skin photoaging diseases are more common, the number of patients is on the rising trend year by year, the skin becomes rough and loose, deep wrinkles appear, other skin diseases such as the occurrence of photosensitive dermatitis and skin cancer can be caused, and the life quality of people is seriously affected.
The mechanism of the occurrence of ultraviolet-induced skin photoaging is not completely understood, and at present, the most accepted statement is that ultraviolet radiation induces abnormal rise of Reactive Oxygen Species (ROS) in skin tissues and causes the occurrence of skin inflammatory reaction, stimulates the release of inflammatory factors, ROS and inflammatory factors act synergistically to cause abnormal expression of Matrix Metalloproteinases (MMPs), has strong extracellular matrix (ECM) degradation capability, and finally causes skin photoaging damage. Under the healthy state of skin, an antioxidant defense system in skin tissues has the capability of removing ROS, the system comprises a plurality of antioxidant enzymes such as superoxide dismutase (SOD), Catalase (CAT) and glutathione peroxidase (GSH-Px), so that ROS and the antioxidant enzymes are in a dynamic balance, the ultraviolet radiation induces abnormal increase of ROS concentration and exceeds the removing capability of the antioxidant system of the body, the oxidation-antioxidant balance in the body is broken, and the oxidative stress pressure in the body is increased, which is the most fundamental reason for causing skin photoaging.
The ocean contains rich biological resources and is closely concerned by people all the time, and the ocean biological active extract has the functions of eliminating free radicals, resisting oxidative damage caused by the free radicals, repairing collagen regeneration and the like and is approved by international famous cosmetic brands. After the French Phytomer company applies the beneficial biological elements contained in the ocean to modern beauty products for the first time and obtains great success, each large international cosmetic company competes for developing own 'ocean series'. Since ancient times, there were cases of women using pearls for beauty treatment and skin care, pearls can make skin moist and tender, and are regarded as good beauty products, and "pearl coating surface is mentioned in Ben Cao gang mu" to make people moist and good in color. Applied to hands and feet, without skin retrogradation. Through detection, more than 95% of the components contained in the pearl are calcium carbonate (which cannot be absorbed by skin), the protein content is about 5%, and the trace elements are between 0.05% and 1%, while the pearl aqueous extract mainly contains protein (17 amino acids can be obtained after hydrolysis, 7 of the amino acids are essential amino acids for human bodies) and a small amount of trace elements, and the pearl aqueous extract has multiple pharmacological effects of oxidation resistance, aging resistance, radiation resistance, inflammation resistance and the like. The skin care product prepared from the pearl can supplement the components required by the skin, promote the metabolism of the skin, prevent the aging of the skin, moisten and smooth the skin, and make the skin tender and white. The marine organism is also another organism, sea grape, the scientific name of caulerpa lentillifera, is rich in a large amount of amino acids, unsaturated fatty acids, polysaccharides, vitamins, minerals and trace elements, and is called as 'green caviar' and 'longevity vegetable'. The sea grape contains rich algal polysaccharide, has the effects of resisting oxidation and aging, beautifying and protecting skin, and other scholars extract and research the fernalin in the sea grape to find that the sea grape has the effects of resisting oxidation and inflammation, so that the value of the sea grape is greatly improved. The Vitis amurensis can be used in cosmetics, and has effects of keeping moisture, increasing skin elasticity, and relieving skin. However, the effect of pearl and sea grape alone on skin photoaging resistance is still not ideal.
Disclosure of Invention
The invention aims to provide a composition for resisting skin photoaging, which has a remarkable effect on resisting skin photoaging.
The technical scheme for solving the problems is as follows:
the skin care composition for resisting skin photoaging comprises effective components and medically acceptable auxiliary materials, and is characterized in that the effective components comprise the following pearl aqueous extract and sea grape extract in percentage by weight: 16-24% of pearl water extract and 76-84% of sea grape extract; wherein the content of the first and second substances,
the pearl aqueous extract is prepared by the following steps: adding appropriate amount of water into Margarita powder, stirring at 4 deg.C, centrifuging, collecting supernatant, dehydrating, and drying to obtain Margarita water extract;
the sea grape extract is prepared by the following method: cleaning Ampelopsis grossedentata to remove salt, squeezing, centrifuging, collecting supernatant, dehydrating, and drying to obtain the Ampelopsis grossedentata extract.
In the scheme, the pearl powder is a commercially available product, such as pearl powder produced by the offshore biological industry of north sea, ltd.
In the scheme, the optimal weight ratio of the pearl aqueous extract to the sea grape extract is as follows: 20% of pearl aqueous extract and 80% of sea grape extract.
The skin care composition of the present invention may be a conventional cream, gel or ointment. The skin care composition can also be skin lotion, skin soap, facial cleanser, facial mask or skin cream according to market demands.
The skin care composition is prepared by adding the effective components into auxiliary materials required by various formulations according to a conventional method, wherein the weight percentage of the effective components is 0.8-1.2%.
Compared with the prior art, the invention has the following beneficial effects:
the pearl aqueous extract and the sea grape extract which are effective components of the skin care composition have a synergistic interaction effect, and the skin photoaging resistance effect is obvious. Meanwhile, the active ingredients of the skin care composition only consist of pearl aqueous extract and sea grape extract, so that the skin care composition is easy to prepare and is beneficial to quality control.
To facilitate a better understanding of the present invention to the public, the following experiments and detailed description further illustrate the beneficial effects of the invention.
Drawings
Fig. 1 is a representative photograph of the macroscopic appearance of the back skin at the ninth week in each group of mice.
FIG. 2 is a histogram of the macroscopic scores of each week for each group of mice; in the figure, A represents blank group mouse macro score, B represents control group 1 mouse macro score, C represents control group 2 mouse macro score, D represents control group 3 mouse macro score, E represents experiment group 1 mouse macro score, F represents experiment group 2 mouse macro score, G represents experiment group 3 mouse macro score; p <0.05 compared to the blank mice macroscopic score.
FIG. 3 is a histogram of the skin lifting time of each group of mice; in the figure, A represents the skin lifting time of a blank group mouse, B represents the skin lifting time of a control group 1 mouse, C represents the skin lifting time of a control group 2 mouse, D represents the skin lifting time of a control group 3 mouse, E represents the skin lifting time of an experiment group 1 mouse, F represents the skin lifting time of an experiment group 2 mouse, and G represents the skin lifting time of an experiment group 3 mouse; p <0.05 compared to the time of skin lifting in the blank group of mice.
Detailed Description
Example 1 (emulsion)
1. The proportion of the effective components is as follows: 0.16g of pearl aqueous extract and 0.84g of sea grape extract.
2. The preparation method comprises the following steps:
(1) taking 500g of pearl powder, adding 12 times of water, stirring at 4 ℃ for 11h, centrifuging at 3500r/min, taking supernatant, and spray drying to obtain 2g of pearl water extract;
(2) soaking 50g of sea grape in clear water and washing for 3 times, each time for 45 minutes, and removing salt; squeezing in a juicer for 5 min, filtering, centrifuging at 3000r/min, and spray drying the supernatant to obtain 5g of the Vitis heyneana extract;
(3) respectively weighing 0.16g of the pearl aqueous extract prepared in the step (1) and 0.84g of the sea grape extract prepared in the step (2), adding 20ml of distilled water, and stirring until the pearl aqueous extract and the sea grape extract are completely dissolved to obtain an effective component mixture;
uniformly mixing 5g of beeswax and 25g of liquid paraffin, and heating to 80 ℃ to prepare an oil phase;
uniformly mixing 2g of polysorbate 80, 0.2g of sorbic acid and 20ml of distilled water, and heating to 80 ℃ to obtain a water phase;
adding the obtained water phase into the obtained oil phase while stirring, stirring to room temperature, adding the obtained effective component mixture, adding distilled water to 100g, and stirring to obtain emulsion.
Example 2 (gelling agent)
1. The proportion of the effective components is as follows: 0.2g of pearl aqueous extract and 0.8g of sea grape extract.
2. The preparation method comprises the following steps:
(1) taking 500g of pearl powder, adding 15 times of water, stirring for 10h at 4 ℃, centrifuging at 5000r/min, taking supernatant, and freeze-drying to obtain 2.2g of pearl water extract;
(2) soaking 50g of sea grape in clear water and washing for 5 times, wherein each time is 25 minutes, and removing salt; squeezing in a juicer for 8 min, filtering, centrifuging at 5000r/min, and lyophilizing the supernatant to obtain extract 6g of Vitis amurensis Rupr;
(3) respectively weighing 0.2g of the pearl aqueous extract prepared in the step (1) and 0.8g of the sea grape extract prepared in the step (2), adding 10ml of distilled water, stirring until the mixture is completely dissolved, then adding 0.5g of swollen carbomer, 0.1g of ethylparaben and 50ml of distilled water, continuously stirring, finally adding distilled water to 100g, and uniformly stirring to obtain the gel.
Example 3 (ointment)
1. The proportion of the effective components is as follows: 0.24g of pearl aqueous extract and 0.76g of sea grape extract.
2. The preparation method comprises the following steps:
(1) taking 500g of pearl powder, adding 10 times of water, stirring at 4 ℃ for 12h, centrifuging at 3000r/min, taking supernatant, and freeze-drying to obtain 2.5g of pearl water extract;
(2) soaking 50g of sea grape in clear water and washing for 4 times, each time for 30 minutes, and removing salt; squeezing in a juicer for 6 min, filtering, centrifuging at 4500r/min, and spray drying the supernatant to obtain the extract 5.5 g;
(3) respectively weighing 0.24g of the pearl aqueous extract prepared in the step (1) and 0.76g of the sea grape extract prepared in the step (2), putting into a mortar for crushing, then mixing and grinding uniformly, adding 20g of glycerin for grinding and dissolving, adding 79g of vaseline in portions, and continuously grinding to obtain the ointment.
Example 4
First, experiment one (drug effect experiment)
(I) test materials
(1) Experimental animals: kunming mouse, weight 20 ~ 22g, provided by the Hunan Slek Jingda laboratory animals Limited, quality certification number: 1107272011006886.
(2) test drug
Control group 1: taking 2g of vitamin E powder, grinding, adding 100ml of glycerol solution for dissolving, and shaking uniformly before use to prepare a vitamin E solution;
control group 2: the vitis amurensis extract is removed according to the mixture ratio of the embodiment 2, and then the gel is prepared according to the method of the embodiment 2.
Control group 3: the pearl aqueous extract is removed according to the proportion of the example 2, and then the gel is prepared according to the method of the example 2.
Experimental group 1: the emulsion prepared in example 1 above was taken.
Experimental group 2: the gel prepared in example 2 above was taken.
Experimental group 3: the ointment prepared in example 3 was collected.
Hydroxyproline (HYP) kit, superoxide dismutase (SOD) kit, glutathione peroxidase (GSH-Px) kit, interleukin 1 beta (IL-1 beta) kit, interleukin 6(IL-6) kit, matrix metalloproteinase 1(MMP-1) kit and the like are purchased from Nanjing winged Fexue Biotech limited.
(II) Experimental method
70 female SPF-level Kunming mice with the weight of 20-22 g are taken, bred in an SPF environment, adaptively bred for a week and then subjected to back skin unhairing treatment by adopting an electric shaver every other day, wherein the unhairing area is about 2.5 multiplied by 3cm2(ii) a Then, 70 mice were randomly divided into a blank group, a control group 1, a control group 2, a control group 3, an experimental group 1, an experimental group 2, and an experimental group 3, each of which was 10 mice. After grouping, coating the corresponding medicine on the back of the mouse, and continuously administering the medicine once a day for nine weeks for each group; the weight of each administration is as follows, the blank group: 20mg/20g distilled water, control 1: 20mg/20g vitamin E, control 2: 10mg/20g pearl aqueous extract, control 2: 10mg/20g of Vitis heyneana extract, Experimental groups 1-3: 10mg/20g of effective components (pearl water extract and sea grape extract). From the day of first administration, mice in each group are given UVA and UVB combined ultraviolet irradiation according to the UVA and UVB radiation dose ratio of 9:1 in week 1-2 and week 4-6, and irradiated once every 2 hours after daily administration; wherein the combined ultraviolet irradiation dose of each time in the first week is 100mJ/cm2The combined ultraviolet irradiation dose of every time in the second week is 200mJ/cm2The combined ultraviolet irradiation dose of each time in the third week is 300mJ/cm2The combined ultraviolet irradiation dose of every four weeks to ninth week is 400mJ/cm2。
(1) Index evaluation
(ii) macroscopic evaluation of skin
During the experiment, the macroscopic characteristics (roughness, wrinkle condition, erythema condition, thickening phenomenon, etc.) of the skin of the back of the mouse, which are related to photoaging, were observed and evaluated. The median skin of the back of the mice was photographed after anesthetizing with ether and the skin was macroscopically scored according to the skin photoaging scoring criteria of table 1 (0-normal skin, 8-severely damaged skin), once a week for a total of nine weeks.
TABLE 1 skin photoaging Scoring criteria
Skin elasticity test: using the skin elasticity test method, after the mice were lightly anaesthetized with ether, the bare skin on the back of the mice was lifted as far as possible along the median line (to the extent that the limbs of the animals were not suspended), and then immediately released, and the time for the skin to return to the state before lifting the skin was recorded. The examination was performed once a week for nine weeks.
(iii) measurement of skin moisture
At the end of the ninth week, the depilated area of the back of each group of mice was about 2.5X 3cm2After a mouse is killed by cervical dislocation, the whole skin (without subcutaneous fat) at the depilated part of the back of the mouse is immediately taken down, about 0.2g of skin is quickly cut, the skin is precisely weighed as wet weight, then the skin is put into an oven and is dried for 4 hours at 105 ℃, the dry weight is weighed, and the water content of the skin of each group of animals is calculated according to the following formula: percent skin moisture (M)(Wet-Dry weight))/MWet weight)×100％。
Measurement of collagen content in skin tissue
Hydroxyproline (Hyp) is a specific amino acid of collagen in the body, and accounts for about 13.4 + -0.24% of collagen of mammals. Therefore, the content of collagen can be calculated by the content of Hyp, and the calculation formula is as follows: the collagen content (μ g/mgprot) ═ Hyp content (μ g/mgprot) × 7.46. Each group of mice was killed by cervical dislocation 2h after the last administration, 0.1g of the back skin (peeled subcutaneous fat) of the mice was precisely weighed, placed in a test tube with a stopper, added with 1ml of 6mol/L HCL, mixed well, capped and placed in a boiling water bath for hydrolysis 5 h. After hydrolysis, the operation is carried out according to the specification of the Hyp kit, the content of Hyp is measured, and the content of collagen is calculated according to a formula.
Measuring the content of antioxidant index in skin tissue
Each group of mice is killed by dislocation of cervical vertebra after last administration for 2h, 0.3g of back skin (peeling subcutaneous fat) of the mice is weighed, the mice are rinsed for 2 times by precooled physiological saline and then are wiped by filter paper, after precise weighing, tissues are cut into pieces under the ice bath condition and then are subpackaged, and the contents of SOD and GSH-Px are respectively determined according to the operation of a kit.
Determination of inflammatory factor content in skin tissue
Sixthly, carrying out cervical dislocation and sacrifice after 2 hours of last administration on each group of mice, weighing 0.3g of back skin (peeling subcutaneous fat) of the mice, rinsing for 2 times by precooled normal saline, wiping out filter paper, precisely weighing, shearing tissues under an ice bath condition, subpackaging, and respectively measuring the content of IL-1 beta and IL-6 according to the operation of an ELISA kit.
Seventhly, measuring the content of matrix metalloproteinase in the skin tissue
Each group of mice was sacrificed by dislocation of cervical vertebrae after 2h of the last administration, 0.2g of back skin (peeled subcutaneous fat) of the mice was weighed, rinsed for 2 times with pre-cooled physiological saline, wiped with filter paper, precisely weighed, cut tissue into pieces under ice bath condition, and subpackaged, and the content of MMP-1 was determined according to the ELISA kit operation.
(2) Statistical examination
Statistical analysis of all data was performed using SPSS 21.0 statistical software, data are in "mean ± SD
"means. The difference between groups was compared by one-way anova, and the two-by-two multiple comparisons within a group were by the LSD method (when the variance was uniform) or the Dunnett's method (when the variance was not uniform). P <0.05 indicates significant difference, and P <0.01 indicates very significant difference.
(III) results of the experiment
(1) Macroscopic improvement of photoaging mouse skin
Representative photographs of the macroscopic appearance of the back skin at the ninth week in each group of mice are shown in fig. 1. After four weeks of uv-irradiation, the mice in the placebo group began to develop typical photoaging macroscopic lesions such as wrinkles, erythema, desquamation, irregular thickening, etc. At the end of the ninth week, the skin of the back of the mice in experimental group 1, experimental group 2, and experimental group 3 appeared healthy and smooth with a small amount of fine wrinkles; the control group 1 occasionally showed a slight erythema lesion and appeared with a small number of visible light wrinkles; the control group 2 and the control group 3 exhibited light aging phenomena such as a small amount of deep wrinkles and slight erythema.
Weekly macroscopic scores were recorded for each group of mice and the results are shown in figure 2. At the end of the ninth week of the experiment, the result of the macroscopic score is shown in table 2, the macroscopic score of the skin of each group of mice is obviously reduced (P is less than 0.01) compared with the blank group, the macroscopic score of the control group 1, the experimental group 2 and the experimental group 3 is not statistically significant (P is more than 0.05) compared with the macroscopic score of the experimental group 1, and the macroscopic score of the skin of the control group 2 and the control group 3 is obviously increased (P is less than 0.01) compared with the skin of the mice of the experimental group 1; the experimental results show that the combination of the pearl aqueous extract and the sea grape extract has an inhibiting effect on the macroscopic damage of the skin induced by ultraviolet radiation.
TABLE 2 macroscale results for animals at week nine: (
n＝10)
(note: as compared to the blank group,*P<0.01, compared with the experimental group 1,▲P<0.01，★P>0.05)
(2) improvement of skin elasticity of photoaged mice
The skin lifting time test can laterally reflect the degree of skin relaxation, so as to evaluate the elasticity of the skin. The skin lifting test results of the mice in each week are shown in figure 3, and the experimental results show that the recovery time of the skin of each group of mice after the fourth week is greatly different. The results of the skin peeling test time at the end of the ninth week are shown in table 3. The recovery time of the skin of each group of mice is obviously reduced compared with that of the blank group (P is less than 0.01), and compared with the control group 1, the experimental group 2 and the experimental group 3, the recovery time of the skin has no statistical significance (P is more than 0.05); compared with the experimental group 1, the skin recovery time of the mice is obviously prolonged in the control group 2 and the control group 3 (P is less than 0.01); the experimental results show that the combination of the pearl aqueous extract and the sea grape extract which are locally administered has obvious treatment effect on the skin elasticity reduction of mice induced by ultraviolet radiation.
TABLE 3 results of skin lifting recovery time of animals in the ninth week
n＝10)
(note: as compared to the blank group,*P<0.01, compared with the experimental group 1,▲P<0.01，★P>0.05)
(3) improve the water content of the skin of the photoaged mouse
The skin of animals is exposed to ultraviolet radiation for a long time, which accelerates the loss of water, causes the skin to be dry and dull, and accelerates the aging of the skin and the generation of wrinkles. The results of the test on the skin moisture content of the mice are shown in table 4, the skin moisture content of the mice in each group is obviously increased (P is less than 0.01) compared with that of the blank group, and the skin moisture content of the mice in the control group 1, the experimental group 2 and the experimental group 3 has no statistical significance (P is more than 0.05) compared with that of the experimental group 1; compared with the experimental group 1, the water content of the skin of the mice in the control group 2 and the control group 3 is obviously reduced (P is less than 0.01); the above experimental results suggest that the combination of the pearl water extract and the sea grape extract can prevent the skin water loss caused by ultraviolet irradiation to a certain extent and improve the hydration ability of the skin.
Table 4 skin moisture content results (
n＝10)
(note: as compared to the blank group,*P<0.01, compared with the experimental group 1,▲P<0.01，★P>0.05)
(4) increasing the content of collagen in skin tissues of photoaged mice
The collagen content results are shown in table 5, the collagen content of the skin of each group of mice is obviously increased (P is less than 0.01) compared with that of the blank group, and the collagen content of the skin of the mice of the control group 1, the experimental group 2, the experimental group 3 and the experimental group 1 has no statistical significance (P is more than 0.05); the collagen of the skin of the mice is obviously reduced (P is less than 0.01) compared with the control group 2 and the control group 3 and the experimental group 1. The above experimental results suggest that the combination of the pearl water extract and the sea grape extract can promote the synthesis of collagen or inhibit the degradation of collagen.
Table 5 skin collagen content results (
n＝10)
(note: as compared to the blank group,*P<0.01, compared with the experimental group 1,▲P<0.01，★P>0.05)
(5) improving the oxidation resistance of skin tissues of photoaged mice
The activity of antioxidant enzymes (such as SOD and GSH-Px) is an important index for evaluating the antioxidant capacity of organisms. As shown in Table 6, the SOD content in the skin of each group of mice is significantly increased (P is less than 0.01) compared with that in the blank group, while the SOD content in the skin of the mice in the control group 1, the experiment group 2, the experiment group 3 and the experiment group 1 has no statistical significance (P is more than 0.05); compared with the experimental group 1, the content of SOD in the skin of the mice is obviously reduced in the control group 2 and the control group 3 (P is less than 0.01).
As shown in Table 6, the content of GSH-Px in the skin of each group of mice is obviously increased (P is less than 0.01) compared with that in the blank group, while the content of antioxidant enzyme in the skin of the mice in the control group 1, the experimental group 2, the experimental group 3 and the experimental group 1 has no statistical significance (P is more than 0.05); compared with the experimental group 1, the content of the GSH-Px in the skin of the mice is obviously reduced in the control group 2 and the control group 3 (P is less than 0.01).
The experimental results indicate that the combination of the pearl water extract freeze-dried powder and the sea grape liquid freeze-dried powder can obviously improve the oxidation resistance of skin tissues, thereby resisting the obvious rise of in vivo oxidative stress pressure induced by ultraviolet radiation.
Table 6 skin antioxidant enzyme results (
n＝10)
(note: as compared to the blank group,*P<0.01, compared with the experimental group 1,▲P<0.01，★P>0.05)
(6) inhibiting the over-expression of inflammatory factors in skin tissue of photoaged mice
Inflammatory factor levels are an important indicator of inflammatory progression. As shown in Table 7, the content of the skin inflammatory factor IL-1 beta of each group of mice is obviously reduced (P is less than 0.01) compared with that of the blank group, and the content of the skin inflammatory factor IL-1 beta of the mice in the control group 1, the experimental group 2, the experimental group 3 and the experimental group 1 has no statistical significance (P is more than 0.05); compared with the experimental group 1, the content of the mouse skin inflammatory factor IL-1 beta in the control group 2 and the control group 3 is obviously increased (P is less than 0.01).
As shown in Table 7, the content of the mouse skin inflammatory factor IL-6 in each group is remarkably reduced (P is less than 0.01) compared with that in the blank group, and the content of the mouse skin inflammatory factor IL-6 in the control group 1, the experimental group 2, the experimental group 3 and the experimental group 1 has no statistical significance (P is more than 0.05); compared with the experimental group 1, the content of the inflammatory factors in the skin of the mice in the control group 2 and the control group 3 is obviously increased (P is less than 0.01).
The experimental results indicate that the combination of the pearl water extract freeze-dried powder and the sea grape liquid freeze-dried powder can inhibit the over-expression of inflammatory factors related to skin photoaging.
Table 7 skin inflammatory factor results (
n＝10)
(note: as compared to the blank group,*P<0.01, compared with the experimental group 1,▲P<0.01，★P>0.05)
(7) inhibiting the abnormal secretion of MMP-1 in the skin tissue of photoaged mice
MMP-1 is the most important enzyme for causing aging symptoms such as wrinkles on the skin, and is also closely related to synthesis and secretion of other metalloproteases. As shown in Table 8, the content of MMP-1 in the skin of mice in each group is significantly reduced (P is less than 0.01) compared with that in the blank group, and the content of MMP-1 in the skin of mice in the control group 1, the experimental group 2 and the experimental group 3 has no statistical significance (P is more than 0.05) compared with that in the experimental group 1; compared with the experimental group 1, the content of MMP-1 in the skin of the mice in the control group 2 and the control group 3 is obviously increased (P is less than 0.01); the experimental results show that the pearl water extract freeze-dried powder and the sea grape liquid freeze-dried powder can be used together to remarkably reduce the abnormal secretion of MMP-1 induced by ultraviolet radiation, thereby inhibiting the excessive degradation of collagen.
TABLE 8 skin MMP-1 results (
n＝10)
(note: as compared to the blank group,*P<0.01, compared with the experimental group 1,▲P<0.01，★P>0.05)
second, experiment two (3)2Analysis of variance of factorial design)
(I) Experimental method
In the experiment, the pearl water extract and the sea grape extract are respectively used as analysis factors, the two-factor three-level factorization design is carried out on each factor by respectively using the respective dosage of the pearl water extract and the sea grape extract in the examples 1 to 3 as one level, and then the variance analysis is carried out on the experimental data. The experimental drugs of the 9 experimental groups were all gels (obviously, emulsions or ointments are also possible), and the specific preparation method was as described in example 2 above; the concrete research method is referred to as the "second experimental method" in the first experiment.
Statistical analysis of all data was performed using SPSS 21.0 statistical software, data are in "mean ± SD
"means; the main effect of the pearl water extract and the sea grape extract and the combined interaction effect of the pearl water extract and the sea grape extract are both 32Researching a cause analysis designed variance analysis method; p <0.05 indicates significant difference, and P <0.01 indicates very significant difference.
(II) results of the experiment
(1) Skin macroscopic scoring
Results from the ninth week experiment are reported in table 9.
TABLE 9 Effect of combination of Pearl aqueous extract and Ampelopsis grossedentata extract on skin Macro-Scoring
The data in table 9 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 10, wherein P of the two main effects is less than 0.01, P of the interaction effect is less than 0.05, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract freeze-dried powder has an effect of inhibiting the ultraviolet radiation-induced skin macroscopic damage.
TABLE 10 analysis of variance table for skin macro-scale scores using combination of pearl water extract and sea grape extract
(2) Time to skin recovery
The results of the ninth week experiment are reported in Table 11.
TABLE 11 Effect of combination of Pearl Water extract and Ampelopsis grossedentata extract on skin recovery time
The data in table 11 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 12, where P of the two main effects is less than 0.01, P of the interaction effects is less than 0.01, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the lyophilized powder of the sea grape aqueous extract has therapeutic effect on the skin elasticity reduction induced by ultraviolet radiation.
TABLE 12 analysis of variance of skin recovery time using combination of pearl water extract and sea grape extract
(3) Water content of photoaged mouse skin
The results of the ninth week experiment are reported in Table 13.
TABLE 13 Effect of combination of Pearl Water extract and Ampelopsis grossedentata extract on skin moisture content
The data in table 13 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 14, where P of the two main effects is less than 0.01, P of the interaction effect is less than 0.05, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract lyophilized powder has therapeutic effect on the skin moisture induced by ultraviolet radiation.
TABLE 14 analysis of variance of skin moisture content using combination of pearl water extract and sea grape extract
(4) Collagen content in skin tissue of photoaged mice
Results from the ninth week experiment are reported in table 15.
TABLE 15 Effect of combination of Pearl aqueous extract and Ampelopsis grossedentata extract on skin collagen
The data in table 15 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 16, wherein P of the two main effects is less than 0.01, P of the interaction effect is less than 0.05, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract freeze-dried powder has therapeutic effect on the decrease of the skin collagen content induced by ultraviolet radiation.
TABLE 16 analysis of variance of skin collagen using the combination of pearl aqueous extract and sea grape extract
(5) Anti-oxidation index of skin tissue of photoaging mouse
Firstly, observing indexes: SOD content
The results of the ninth week experiment are reported in Table 17.
TABLE 17 Effect of combination of Pearl Water extract and Ampelopsis grossedentata extract on skin SOD content
The data in table 17 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 18, wherein P of the two main effects is less than 0.01, P of the interaction effect is less than 0.05, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract lyophilized powder has therapeutic effect on the decrease of skin SOD content induced by ultraviolet radiation.
TABLE 18 analysis of variance of skin SOD content using combination of pearl water extract and sea grape extract
Observation indexes: GSH-Px content
Results from the ninth week experiment are reported in table 19.
TABLE 19 Effect of combination of Pearl Water extract and sea grape extract on skin GSH-Px content
The data in table 19 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 20, wherein P of the two main effects is less than 0.01, P of the interaction effect is less than 0.05, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract lyophilized powder has therapeutic effect on the reduction of the skin GSH-Px content induced by ultraviolet radiation.
TABLE 20 analysis of variance of skin GSH-Px content using combination of pearl water extract and sea grape extract
(6) Inflammatory factors in skin tissue of photoaged mice
Firstly, observing indexes: IL-1 beta content
The results of the ninth week experiment are reported in Table 21.
TABLE 21 Effect of combination of Pearl Water extract and Ampelopsis grossedentata extract on the IL-1. beta. content of skin
The data in table 21 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 22, wherein P of the two main effects is less than 0.01, P of the interaction effect is less than 0.05, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract lyophilized powder has therapeutic effect on the decrease of skin SOD content induced by ultraviolet radiation.
TABLE 22 analysis of variance of skin IL-1 beta content using combination of pearl aqueous extract and sea grape extract
Observation indexes: IL-6 content
The results of the ninth week experiment are reported in table 23.
TABLE 23 Effect of combination of Pearl Water extract and Ampelopsis grossedentata extract on the IL-6 content of skin
The data in table 23 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 24, wherein P of the two main effects is less than 0.01, P of the interaction effect is less than 0.01, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract freeze-dried powder has an inhibitory effect on the increase of the content of skin IL-6 induced by ultraviolet radiation.
TABLE 24 analysis of variance of skin IL-6 content using combination of pearl water extract and sea grape extract
(7) MMP-1 in skin tissue of photoaged mice
Results from the ninth week experiment are reported in table 25.
TABLE 25 Effect of combination of Pearl Water extract and sea grape extract on skin MMP-1
The data in table 25 were subjected to statistical analysis of the main effects and the interaction effects of the factors, and the results are shown in table 26, wherein P of the two main effects is less than 0.01, P of the interaction effect is less than 0.05, and the differences have statistical significance, which indicates that the interaction between the pearl aqueous extract and the sea grape aqueous extract freeze-dried powder has an inhibitory effect on the increase of the content of MMP-1 in the skin induced by ultraviolet radiation.
TABLE 26 ANOVA TABLE OF COMBINATION OF ZHENZHU WATER EXTRACT AND HAIMETA EXTRACT FOR SKIN MMP-1
Third, conclusion of experiment
In conclusion, the combination of the pearl aqueous extract and the sea grape extract has a synergistic effect on the anti-skin photoaging aspect, the anti-skin photoaging effect of the mouse skin photoaging composition is obviously better than that of the single pearl aqueous extract and the sea grape extract, and the skin of the photoaging mouse can be protected from being damaged by ultraviolet radiation.
Claims (4)
1. The skin care composition for resisting skin photoaging comprises effective components and medically acceptable auxiliary materials, and is characterized in that the effective components comprise pearl aqueous extract and sea grape extract in the following weight percentages: 16-24% of pearl water extract and 76-84% of sea grape extract; wherein the content of the first and second substances,
the pearl aqueous extract is prepared by the following steps: adding appropriate amount of water into Margarita powder, stirring at 4 deg.C, centrifuging, collecting supernatant, dehydrating, and drying to obtain Margarita water extract;
the sea grape extract is prepared by the following method: cleaning Ampelopsis grossedentata to remove salt, squeezing, centrifuging, collecting supernatant, dehydrating, and drying to obtain the Ampelopsis grossedentata extract.
2. The skin care composition for resisting skin photoaging, according to claim 1, wherein the effective components comprise the following components in percentage by weight: 20% of pearl aqueous extract and 80% of sea grape extract.
3. The skin care composition according to claim 1 or 2, wherein the active ingredient is present in an amount of 0.8 to 1.2% by weight of the skin care composition.
4. The skin care composition of claim 2, wherein said skin care composition is a cream, gel or ointment.
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