CN1152135C - 酶的固定化方法 - Google Patents

酶的固定化方法 Download PDF

Info

Publication number
CN1152135C
CN1152135C CNB988126494A CN98812649A CN1152135C CN 1152135 C CN1152135 C CN 1152135C CN B988126494 A CNB988126494 A CN B988126494A CN 98812649 A CN98812649 A CN 98812649A CN 1152135 C CN1152135 C CN 1152135C
Authority
CN
China
Prior art keywords
enzyme
carrier
reactant
lipase
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB988126494A
Other languages
English (en)
Chinese (zh)
Other versions
CN1283226A (zh
Inventor
M��W������˹̹ɭ
M·W·克里斯坦森
O·科克
�������ɭ
C·派德森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of CN1283226A publication Critical patent/CN1283226A/zh
Application granted granted Critical
Publication of CN1152135C publication Critical patent/CN1152135C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier

Landscapes

  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CNB988126494A 1997-12-23 1998-12-16 酶的固定化方法 Expired - Fee Related CN1152135C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK1527/1997 1997-12-23
DK152797 1997-12-23

Publications (2)

Publication Number Publication Date
CN1283226A CN1283226A (zh) 2001-02-07
CN1152135C true CN1152135C (zh) 2004-06-02

Family

ID=8105764

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB988126494A Expired - Fee Related CN1152135C (zh) 1997-12-23 1998-12-16 酶的固定化方法

Country Status (8)

Country Link
EP (1) EP1042458B1 (enExample)
JP (1) JP4307713B2 (enExample)
CN (1) CN1152135C (enExample)
AT (1) ATE254170T1 (enExample)
AU (1) AU1556699A (enExample)
DE (1) DE69819782T2 (enExample)
DK (1) DK1042458T3 (enExample)
WO (1) WO1999033964A1 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6406723B1 (en) 1997-04-09 2002-06-18 Danisco A/S Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase
ATE231186T1 (de) 1998-07-21 2003-02-15 Danisco Lebensmittel
US6268191B1 (en) * 1998-09-21 2001-07-31 Robert K. Prud'homme Enzyme immobilization by imbibing an enzyme solution into dehydrated hydrocolloid gel beads
DE10046039A1 (de) * 2000-09-18 2002-03-28 Basf Ag Polykondensation von organischen Siliciumverbindungen
WO2002094123A2 (en) 2001-05-18 2002-11-28 Danisco A/S Method of preparing a dought with an enzime
US20040130968A1 (en) * 2002-10-09 2004-07-08 Novozymes A/S Method for improving particle compositions
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
AU2005264938B2 (en) * 2004-06-16 2011-11-24 Verenium Corporation Compositions and methods for enzymatic decolorization of chlorophyll
CN102533440B (zh) 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
EP1859035B1 (en) * 2005-03-08 2016-01-13 Agency for Science, Technology and Research Immobilised enzymes
CA2611723C (en) 2005-06-09 2014-02-11 The Nisshin Oillio Group, Ltd. Lipase powder compositions
WO2007036235A1 (en) 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes
IL180598A0 (en) * 2007-01-08 2007-07-04 Basheer Sobhi Immobilized interfacial enzymes of improved and stabilized activity
CA2673954C (en) 2007-01-25 2015-09-15 Danisco A/S Production of a lipid acyltransferase from transformed bacillus licheniformis cells
IL183084A0 (en) 2007-05-09 2007-09-20 Trans Biodisel Ltd Modified-immobilized enzymes of high tolerance to hydrophilic substrates in organic media
DE102008006716A1 (de) * 2008-01-30 2009-08-13 BAM Bundesanstalt für Materialforschung und -prüfung Lipasenformulierung
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
DK3004344T3 (en) * 2013-05-31 2018-11-26 Dsm Ip Assets Bv IMMOBILIZED PROLINE SPECIFIC ENDOPROTEASE
CN104593349B (zh) * 2015-01-14 2018-06-22 华南理工大学 一种gy2b降解菌固定化小球及其制备方法与应用
CN106811455A (zh) * 2015-11-27 2017-06-09 丰益(上海)生物技术研发中心有限公司 固定化酶及其制备方法
RU2020120682A (ru) 2017-11-24 2021-12-24 Новозимс А/С Мелкие частицы на основе фермента для переэтерификации
EP3744838B1 (en) 2019-05-29 2026-07-08 Novozymes A/S Lipolytic polymer particles for esterification and interesterification
BR112023017230A2 (pt) 2021-02-26 2023-09-26 Novozymes As Partículas de poliestireno/divinilbenzeno para a imobilização de lipase
CN116240082B (zh) * 2023-02-21 2024-03-19 天津科技大学 一种基于固定化酶催化的黄水利用技术
AU2024363779A1 (en) 2023-10-16 2026-03-05 Novozymes A/S Method of producing interesterified fat product

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK402583D0 (da) * 1983-09-05 1983-09-05 Novo Industri As Fremgangsmade til fremstilling af et immobiliseret lipasepraeparat og anvendelse deraf
KR950702625A (ko) * 1993-05-18 1995-07-29 한스 발터 라벤 비살포성 효소의 제조방법(process for dust-free enzyme manufacture)
DE69531538T2 (de) * 1994-02-21 2004-06-24 Novozymes A/S Verfahren zur herstellung einer immobilisierten enzympräparation und ihre verwendung

Also Published As

Publication number Publication date
CN1283226A (zh) 2001-02-07
DE69819782D1 (de) 2003-12-18
DE69819782T2 (de) 2004-09-30
DK1042458T3 (da) 2004-03-22
AU1556699A (en) 1999-07-19
WO1999033964A1 (en) 1999-07-08
ATE254170T1 (de) 2003-11-15
JP4307713B2 (ja) 2009-08-05
JP2002500007A (ja) 2002-01-08
EP1042458B1 (en) 2003-11-12
EP1042458A1 (en) 2000-10-11

Similar Documents

Publication Publication Date Title
JP4307713B2 (ja) 酵素の固定化方法
US6156548A (en) Immobilization of enzymes with a fluidized bed for use in an organic medium
RESLOW et al. On the importance of the support material for bioorganic synthesis: Influence of water partition between solvent, enzyme and solid support in water‐poor reaction media
Shaw et al. Lipolytic activities of a lipase immobilized on six selected supporting materials
Cunha et al. Preparation of core–shell polymer supports to immobilize lipase B from Candida antarctica: effect of the support nature on catalytic properties
Bosley et al. Immobilization of lipases on porous polypropylene: reduction in esterification efficiency at low loading
JP2873251B2 (ja) 粒状固定化リパーゼ調製品、その製法およびその使用
KR100774281B1 (ko) 불용성 기질에 고정된 계면활성제-리파아제 복합물
WO2009010561A1 (en) Immobilization of enzymes
SK176397A3 (en) A method for immobilization of enzymes
Sánchez‐Otero et al. Polypropylene as a selective support for the immobilization of lipolytic enzymes: hyper‐activation, purification and biotechnological applications
CN106929501B (zh) 脂肪酶固定化载体、固定化脂肪酶及其制备方法和应用
WO2015081879A1 (zh) 催化酯化和酯交换的Sn-1,3选择性固定化脂肪酶及其制备方法
Kumar et al. Effective immobilisation of lipase to enhance esterification potential and reusability
Panzavolta et al. Acetylenic polymers as new immobilization matrices for lipolytic enzymes
MX2007014143A (es) Metodo para producir grasas o aceites.
CN102016019B (zh) 固定化酶的制造方法
CN116024197B (zh) 一种碳化花粉固定化脂肪酶及其制备方法
CN106811455A (zh) 固定化酶及其制备方法
CN112301025A (zh) 固定化酶载体的修饰方法及应用
JP2657887B2 (ja) 固定化酵素の調製方法
JPH0585157B2 (enExample)
CN113388601A (zh) 酶的制造方法及酶
CN113939589A (zh) 用于酯化和酯交换的脂肪分解聚合物颗粒
JP2676470B2 (ja) 固定化リパーゼ、その製造方法及び該リパーゼを用いた油脂類のエステル交換方法

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Owner name: NUOWOQIMEIZI CO.,LTD.

Free format text: FORMER OWNER: NOVO NORDISK A/S

Effective date: 20021212

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20021212

Address after: Denmark bagsvaerd

Applicant after: Novo Jymes A/S

Address before: Denmark bagsvaerd

Applicant before: Novo Nordisk A/S

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1080732

Country of ref document: HK

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20040602

Termination date: 20171216