JP4307713B2 - 酵素の固定化方法 - Google Patents

酵素の固定化方法 Download PDF

Info

Publication number
JP4307713B2
JP4307713B2 JP2000526622A JP2000526622A JP4307713B2 JP 4307713 B2 JP4307713 B2 JP 4307713B2 JP 2000526622 A JP2000526622 A JP 2000526622A JP 2000526622 A JP2000526622 A JP 2000526622A JP 4307713 B2 JP4307713 B2 JP 4307713B2
Authority
JP
Japan
Prior art keywords
enzyme
carrier
liquid
hydrophilic
fluidized bed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2000526622A
Other languages
English (en)
Japanese (ja)
Other versions
JP2002500007A (ja
JP2002500007A5 (enExample
Inventor
ウルツ クリステンセン，モルテン
キルク，オレ
ペデルセン，クリスチャン
Original Assignee
ノボザイムス アクティーゼルスカブ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ノボザイムス アクティーゼルスカブ filed Critical ノボザイムス アクティーゼルスカブ
Publication of JP2002500007A publication Critical patent/JP2002500007A/ja
Publication of JP2002500007A5 publication Critical patent/JP2002500007A5/ja
Application granted granted Critical
Publication of JP4307713B2 publication Critical patent/JP4307713B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier

Landscapes

  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2000526622A 1997-12-23 1998-12-16 酵素の固定化方法 Expired - Fee Related JP4307713B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK152797 1997-12-23
DK1527/97 1997-12-23
PCT/DK1998/000554 WO1999033964A1 (en) 1997-12-23 1998-12-16 A process for immobilisation of enzymes

Publications (3)

Publication Number Publication Date
JP2002500007A JP2002500007A (ja) 2002-01-08
JP2002500007A5 JP2002500007A5 (enExample) 2006-02-09
JP4307713B2 true JP4307713B2 (ja) 2009-08-05

Family

ID=8105764

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000526622A Expired - Fee Related JP4307713B2 (ja) 1997-12-23 1998-12-16 酵素の固定化方法

Country Status (8)

Country Link
EP (1) EP1042458B1 (enExample)
JP (1) JP4307713B2 (enExample)
CN (1) CN1152135C (enExample)
AT (1) ATE254170T1 (enExample)
AU (1) AU1556699A (enExample)
DE (1) DE69819782T2 (enExample)
DK (1) DK1042458T3 (enExample)
WO (1) WO1999033964A1 (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
EP1466980B1 (en) 1997-04-09 2006-06-28 Danisco A/S Lipase and use of same for improving doughs and baked products
ES2188190T5 (es) 1998-07-21 2007-11-16 Danisco A/S Producto alimentario.
US6268191B1 (en) * 1998-09-21 2001-07-31 Robert K. Prud'homme Enzyme immobilization by imbibing an enzyme solution into dehydrated hydrocolloid gel beads
DE10046039A1 (de) * 2000-09-18 2002-03-28 Basf Ag Polykondensation von organischen Siliciumverbindungen
ATE362315T1 (de) 2001-05-18 2007-06-15 Danisco Verfahren zur herstellung von teig mit einem enzym
US20040130968A1 (en) * 2002-10-09 2004-07-08 Novozymes A/S Method for improving particle compositions
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
DE602004030000D1 (de) 2003-01-17 2010-12-23 Danisco Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
PL2468853T3 (pl) * 2004-06-16 2015-08-31 Dsm Ip Assets Bv Sposób enzymatycznego odbarwiania feofityny
EP2267107A3 (en) 2004-07-16 2011-05-25 Danisco A/S Lipolytic Enzyme. Uses Thereof In The Food Industry
US8715982B2 (en) * 2005-03-08 2014-05-06 Agency For Science, Technology And Research Immobilised enzymes
CN101194014B (zh) * 2005-06-09 2011-10-19 日清奥利友集团株式会社 脂肪酶粉末组合物
EP1934342B1 (en) 2005-09-30 2014-12-10 Novozymes A/S Immobilization of enzymes
IL180598A0 (en) * 2007-01-08 2007-07-04 Basheer Sobhi Immobilized interfacial enzymes of improved and stabilized activity
DK2405007T5 (da) 2007-01-25 2014-06-23 Dupont Nutrition Biosci Aps Fremstilling af en lipid acyltransferase ud af transformerede Bacillus licheniformis-celler
IL183084A0 (en) 2007-05-09 2007-09-20 Trans Biodisel Ltd Modified-immobilized enzymes of high tolerance to hydrophilic substrates in organic media
DE102008006716A1 (de) * 2008-01-30 2009-08-13 BAM Bundesanstalt für Materialforschung und -prüfung Lipasenformulierung
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
JP2016522688A (ja) * 2013-05-31 2016-08-04 ディーエスエム アイピー アセッツ ビー．ブイ． 固定化プロリン特異的エンドプロテアーゼ
CN104593349B (zh) * 2015-01-14 2018-06-22 华南理工大学 一种gy2b降解菌固定化小球及其制备方法与应用
CN106811455A (zh) * 2015-11-27 2017-06-09 丰益(上海)生物技术研发中心有限公司 固定化酶及其制备方法
CN111630161A (zh) 2017-11-24 2020-09-04 诺维信公司 用于酯交换的小的酶颗粒
EP3744838A1 (en) 2019-05-29 2020-12-02 Novozymes A/S Lipolytic polymer particles for esterification and interesterification
JP2024507907A (ja) 2021-02-26 2024-02-21 ノボザイムス アクティーゼルスカブ リパーゼ固定用のポリスチレン／ジビニルベンゼン粒子
CN116240082B (zh) * 2023-02-21 2024-03-19 天津科技大学 一种基于固定化酶催化的黄水利用技术
WO2025083001A2 (en) 2023-10-16 2025-04-24 Novozymes A/S Method of producing interesterified fat product

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK402583D0 (da) * 1983-09-05 1983-09-05 Novo Industri As Fremgangsmade til fremstilling af et immobiliseret lipasepraeparat og anvendelse deraf
WO1994026883A2 (en) * 1993-05-18 1994-11-24 Gist-Brocades N.V. Process for dust-free enzyme manufacture
AU1806595A (en) * 1994-02-21 1995-09-04 Novo Nordisk A/S Method for production of an immobilized enzyme preparation and use of the immobilized enzyme preparation

Also Published As

Publication number Publication date
EP1042458A1 (en) 2000-10-11
EP1042458B1 (en) 2003-11-12
DK1042458T3 (da) 2004-03-22
JP2002500007A (ja) 2002-01-08
CN1283226A (zh) 2001-02-07
DE69819782D1 (de) 2003-12-18
WO1999033964A1 (en) 1999-07-08
AU1556699A (en) 1999-07-19
CN1152135C (zh) 2004-06-02
DE69819782T2 (de) 2004-09-30
ATE254170T1 (de) 2003-11-15

Similar Documents

Publication Publication Date Title
JP4307713B2 (ja) 酵素の固定化方法
US6156548A (en) Immobilization of enzymes with a fluidized bed for use in an organic medium
Bosley et al. Blueprint for a lipase support: Use of hydrophobic controlled‐pore glasses as model systems
Bosley et al. Immobilization of lipases on porous polypropylene: reduction in esterification efficiency at low loading
RESLOW et al. On the importance of the support material for bioorganic synthesis: Influence of water partition between solvent, enzyme and solid support in water‐poor reaction media
de Oliveira et al. Immobilisation studies and catalytic properties of microbial lipase onto styrene–divinylbenzene copolymer
JP2873251B2 (ja) 粒状固定化リパーゼ調製品、その製法およびその使用
Bayramoğlu et al. Immobilization of Candida rugosa lipase onto spacer-arm attached poly (GMA-HEMA-EGDMA) microspheres
JP2862509B2 (ja) リパーゼ固定化用担体及び固定化リパーゼ
WO2009010561A1 (en) Immobilization of enzymes
Ghiaci et al. Enzyme immobilization: Part 2: Immobilization of alkaline phosphatase on Na-bentonite and modified bentonite
JP2002500007A5 (enExample)
Arica et al. Reversible immobilization of lipase on phenylalanine containing hydrogel membranes
JPS6216637B2 (enExample)
Kumar et al. Effective immobilisation of lipase to enhance esterification potential and reusability
Panzavolta et al. Acetylenic polymers as new immobilization matrices for lipolytic enzymes
Suckling Immobilized enzymes
US4581338A (en) Preparation of catalyst supports and materials produced thereby
US20130183734A1 (en) Phospholipase-Carrier Complex
JPH0585157B2 (enExample)
EP3196315B1 (en) Spherical pellets, manufacturing process of such pellets, use, and a detection tube comprising such pellets
JP2676470B2 (ja) 固定化リパーゼ、その製造方法及び該リパーゼを用いた油脂類のエステル交換方法
Bhushan et al. Macroporous beads for lipase immobilization: kinetic resolution of a racemic drug intermediate
JPH01257485A (ja) 酵素によるトリグリセライドの合成方法
JPS6349996B2 (enExample)

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20051213

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20051213

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20080530

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20080909

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20081208

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20081215

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090306

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20090331

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20090430

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120515

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20120515

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130515

Year of fee payment: 4

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130515

Year of fee payment: 4

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees