CN115192472A - Whitening essence containing ionic liquid humanized type III collagen and preparation method thereof - Google Patents
Whitening essence containing ionic liquid humanized type III collagen and preparation method thereof Download PDFInfo
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Abstract
The invention discloses whitening essence milk containing ionic liquid humanized III type collagen and a preparation method thereof. In drug delivery, ILs have the effects of increasing drug solubility, improving drug stability, promoting drug permeability and the like, so that the permeability of alpha-arbutin in skin can be improved by adding the ionic liquid humanized type III collagen into the whitening essence milk, the addition amount of the alpha-arbutin is reduced on the premise of the same whitening effect of the whitening essence milk, high bioavailability at a lower concentration is realized, and continuous administration and sustained release effects are endowed.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to whitening essence milk containing ionic liquid humanized type III collagen and a preparation method thereof.
Background
Arbutin is a natural active substance extracted from various plants, is a glucosylated hydroquinone derivative, belongs to hydroquinone glucoside class in compound classification, has certain specific physiological functions, and is widely present in animal, plant and microbial cells. And arbutin has two isomers, alpha-arbutin and beta-arbutin. Research shows that the alpha-arbutin is far stronger than the beta-arbutin in the aspect of whitening efficacy, and the alpha-arbutin is far better than the beta-arbutin in the aspect of use safety.
The alpha-arbutin is a hydroquinone substance glycosylated under certain conditions, easily-broken glycosidic bonds are formed between glucose molecules and hydroquinone in a molecular structure, and after the glycosidic bonds are broken, arbutin can be decomposed to generate the hydroquinone substance; hydroquinone belongs to a phenolic compound and has toxicity, and when a small amount of hydroquinone is taken by mistake, a person can have symptoms of headache, dizziness, tinnitus, pale complexion and the like, and the hydroquinone can cause toxic action on skin cells when contacting the skin.
Hydroquinone is a starting material for synthesizing arbutin, after arbutin is decomposed under the action of enzyme existing in skin cells, trace hydroquinone is generated, pathological effects can be generated after the arbutin is used for a period of time, generally, cosmetics can achieve better whitening curative effects, wherein the dosage of arbutin is preferably 3%, when the dosage is lower than the value, the effect of arbutin on inhibiting tyrosinase catalytic activity in skin is not obvious, and when the dosage is more than 3%, the result that hydroquinone damages skin can be caused. However, the existing whitening products have no effects of ensuring whitening effect, safety and economy.
Accordingly, the prior art is yet to be improved and developed.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a whitening essence milk containing ionic liquid humanized type III collagen and a preparation method thereof, and aims to solve the problems of high content of alpha-arbutin and low bioavailability of the whitening product in the prior art.
The technical scheme of the invention is as follows:
the whitening essence milk containing the ionic liquid humanized type III collagen is characterized by comprising the following components in percentage by mass:
1-4% of emulsifier, 0.1-1% of emulsion stabilizer, 1-25% of emollient, 2-20% of humectant, 0.1-2% of thickener, 0.01-0.3% of aromatizer, 1-4% of ionic liquid humanized III type collagen, 1-2% of alpha-arbutin and the balance of water;
the ionic liquid humanized III type collagen is prepared by the reaction of humanized III type collagen and L-carnitine citrate ionic liquid.
The whitening essence containing the ionic liquid humanized type III collagen is characterized in that the emulsifier is at least one selected from C20-22 alcohol phosphate, tween-60, PEG-20 methyl glucose sesquistearate, polyglycerol-6 laurate, cetearyl glucoside, polyglycerol-10 isostearate and methyl glucose sesquistearate; the emulsion stabilizer is at least one selected from hexadecanol, 22 alcohol, PEG-100 stearate and glycerol stearate; the emollient is selected from squalane, jojoba oil, coco-caprylate/caprate, macadamia nut oil, camellia oil, hydrogenated polyisobutene, dimethiconol, vitamin E acetate, dimethicone, isononyl isononanoate, caprylic/capric triglyceride, and C12-15 alcohol benzoate.
The whitening essence milk containing the ionic liquid humanized type III collagen is characterized in that the humectant is one selected from glycerol, glycerol glucoside, butanediol, propylene glycol, sorbitol, betaine, diglycerol and sodium hyaluronate; the thickening agent is selected from one of sphingomonas ferment product extract, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyl taurate/VP copolymer, polyacrylamide, polyacrylate cross-linked polymer-6, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the aromatizer is at least one of essence, essential oil and flower water.
The whitening essence milk containing the ionic liquid humanized type III collagen consists of 2.5 percent of C20-22 alcohol phosphate, 0.6 percent of hexadecanol, 4 percent of caprylic/capric triglyceride, 4 percent of isononyl isononanoate, 3 percent of hydrogenated polyisobutene, 1 percent of vitamin E acetate, 0.2 percent of Tween-60, 3 percent of polydimethylsiloxane, 1 percent of dimethiconol, 6 percent of glycerol, 3 percent of betaine, 1.5 percent of glycerol glucoside, 0.5 percent of polyacrylamide dimethyl taurine, 0.15 percent of sphingomonas ferment extract, 2 percent of diglycerol, 0.02 percent of essence, 3 percent of the ionic liquid humanized type III collagen, 2 percent of alpha-arbutin and the balance of water according to mass percentage.
A preparation method of whitening essence milk containing ionic liquid humanized type III collagen comprises the following steps:
stirring and mixing C20-22 alcohol phosphate, cetostearyl alcohol, caprylic/capric triglyceride, isononyl isononanoate, hydrogenated polyisobutene, vitamin E acetate, tween-60, polydimethylsiloxane and dimethiconol at a first temperature to obtain a first mixture;
adding glycerol, betaine, glycerol glucoside, sodium polyacryloyldimethyl taurate, sphingomonas fermentation product extract and diglycerol into water, and stirring and dissolving at a first temperature to obtain a second mixture;
adding the first mixture into the second mixture, homogenizing, then stirring and cooling to a second temperature, adding the ionic liquid humanized type III collagen and the alpha-arbutin, and continuing stirring;
and finally, adding essence, stirring and cooling to a third temperature to prepare the whitening essence milk containing the ionic liquid humanized III type collagen.
The preparation method of the whitening essence milk containing the ionic liquid humanized type III collagen comprises the following steps of (1) controlling the first temperature to be 80-85 ℃; the second temperature is less than 50 ℃; the third temperature is 35-40 ℃.
The preparation method of the whitening essence milk containing the ionic liquid humanized type III collagen comprises the following steps:
providing an L-carnitine citrate ionic liquid, wherein the structural formula of the L-carnitine citrate ionic liquid is as follows:
and mixing the L-carnitine citrate ionic liquid and the humanized III type collagen solution, stirring, and then sequentially homogenizing, filtering, dialyzing and concentrating to obtain the ionic liquid humanized III type collagen.
The preparation method of the whitening essence milk containing the ionic liquid humanized type III collagen comprises the following steps of (1) preparing a mixture of L-carnitine citrate ionic liquid and humanized type III collagen according to a mass ratio of 10.
The preparation method of the whitening essence milk containing the ionic liquid and the humanized type III collagen comprises the following steps of mixing an L-carnitine citrate ionic liquid and a humanized type III collagen solution, and specifically comprises the following steps:
adding the humanized III type collagen solution into the L-carnitine citrate ionic liquid within 10-60 minutes under the conditions of 50-300 rpm stirring speed and water bath at-5-40 ℃.
The preparation method of the whitening essence milk containing the ionic liquid humanized type III collagen comprises the following homogenization treatment conditions: the pressure is 0-80MPa, the flow is 30-60L/h, and the homogenizing time is 3-5min.
Has the beneficial effects that: the invention provides a whitening essence milk containing ionic liquid humanized III type collagen and a preparation method thereof, wherein the Ionic Liquid (ILs) humanized III type collagen in the whitening essence milk containing the ionic liquid humanized III type collagen has the characteristics of low volatility, high stability, high conductivity, permeability promotion and the like, and the characteristics conform to the pharmaceutical concept of 'green chemistry'. In drug delivery, ILs have the effects of increasing drug solubility, improving drug stability, promoting drug permeability and the like, so that the permeability of alpha-arbutin in skin can be improved by adding the ionic liquid humanized type III collagen into the whitening essence milk, the addition amount of the alpha-arbutin is reduced on the premise of the same whitening effect of the whitening essence milk, high bioavailability at a lower concentration is realized, and continuous administration and sustained release effects are endowed.
Drawings
FIG. 1 is a bar graph of the MI values of melanin at D0 and D28 for comparative example 1 and examples 1-5 of the present invention;
FIG. 2 is a bar graph showing MI differences of melanin in comparative example 1 and examples 1 to 5 according to the present invention;
FIG. 3 is a bar graph of the ratio of mottle area at D0 to D28 for comparative example 1 and examples 1-5 of the present invention;
FIG. 4 is a bar graph of the area fraction difference of the color patches of comparative example 1 and examples 1-5 of the present invention.
Detailed Description
The present invention provides a whitening essence milk containing an ionic liquid humanized type III collagen and a preparation method thereof, and the present invention is further described in detail below in order to make the objects, technical solutions and effects of the present invention clearer and clearer. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
In the embodiments and claims, the articles "a", "an", "the" and "the" may include plural forms as well, unless the context specifically dictates otherwise. If there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature.
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The molecular structure of arbutin has two structural and functional groups: one is a glucose residue; the second is phenolic hydroxyl. Therefore, the principle of arbutin whitening is that the glucose residue has hydrophilicity and the phenolic hydroxyl group has the function of melanin synthase, so that the arbutin whitening additive can play a whitening role to a certain extent when being used as a whitening additive playing a whitening role and added into the whitening additive. And arbutin has two isomers, alpha-arbutin and beta-arbutin. Research shows that the alpha-arbutin is far stronger than the beta-arbutin in the aspect of whitening efficacy, and the alpha-arbutin is far better than the beta-arbutin in the aspect of use safety.
However, the alpha-arbutin content in the existing whitening essence milk generally needs to be added to more than 3% to have a good whitening effect, but when the dosage of the alpha-arbutin is more than 3%, certain safety risk exists, and skin damage can be caused seriously; and when the content is less than 3 percent, the effect of the alpha-arbutin on inhibiting the tyrosinase catalytic activity in the skin is not obvious.
Therefore, it is needed to reduce the addition amount of alpha-arbutin and ensure the safety and economy of the whitening essence milk on the premise of the same whitening effect.
Based on the above, the invention provides a whitening essence milk containing ionic liquid humanized type III collagen, which comprises the following components in percentage by mass:
1-4% of emulsifier, 0.1-1% of emulsion stabilizer, 1-25% of emollient, 2-20% of humectant, 0.1-2% of thickener, 0.01-0.3% of aromatizer, 1-4% of ionic liquid humanized type III collagen, 1-2% of alpha-arbutin and the balance of water; (ii) a
The ionic liquid humanized III type collagen is prepared by the reaction of humanized III type collagen and L-carnitine citrate ionic liquid.
In drug delivery, ionic Liquids (ILs) have the effects of increasing drug solubility, improving drug stability, promoting drug permeability and the like, and ILs can also serve as a pharmaceutical active ingredient to form a novel drug delivery system of API-ILs. In addition, the natural biological ILs of the L-carnitine and the citric acid can realize the effects of resisting oxidation, removing cutin and the like. Therefore, the invention makes the humanized III type collagen react with the L-carnitine citrate ionic liquid to prepare the ionic liquid humanized III type collagen, improves the bioavailability of the alpha-arbutin by utilizing the functions of improving the stability, promoting the permeability and the like of the ionic liquid humanized III type collagen, can reduce the addition amount of the alpha-arbutin in the whitening essence milk on the premise of the same whitening effect, realizes high bioavailability at lower concentration, and endows the effects of continuous administration and sustained release.
In some embodiments, the emulsifier is selected from at least one of C20-22 alcohol phosphate, tween-60, PEG-20 methylglucamine sesquistearate, polyglycerol-6 laurate, cetearyl glucoside, polyglycerol-10 isostearate, methylglucamine sesquistearate; the emulsion stabilizer is at least one selected from the group consisting of cetostearyl alcohol, 22 alcohol, PEG-100 stearate and glyceryl stearate; the emollient is selected from squalane, jojoba oil, coco-caprylate/caprate, macadamia nut oil, camellia oil, hydrogenated polyisobutene, dimethiconol, vitamin E acetate, dimethicone, isononyl isononanoate, caprylic/capric triglyceride, and C12-15 alcohol benzoate.
In some embodiments, the humectant is selected from one of glycerin, glycerol glucoside, butylene glycol, propylene glycol, sorbitol, betaine, diglycerin, sodium hyaluronate; the thickening agent is selected from one of sphingomonas ferment product extract, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyl taurate/VP copolymer, polyacrylamide, polyacrylate cross-linked polymer-6, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the aromatizer is at least one of essence, essential oil and flower water.
In some embodiments, the whitening essence containing ionic liquid humanized type III collagen consists of, by mass percent, C20-22 alcohol phosphate 2.5%, cetostearyl alcohol 0.6%, caprylic/capric triglyceride 4%, isononyl isononanoate 4%, hydrogenated polyisobutene 3%, vitamin E acetate 1%, tween-60.2%, polydimethylsiloxane 3%, dimethiconol 1%, glycerin 6%, betaine 3%, glycerol glucoside 1.5%, sodium polyacryloyldimethyltaurinate 0.5%, sphingomonas bacterium fermentation product extract 0.15%, diglycerin 2%, essence 0.02%, ionic liquid humanized type III collagen 3%, alpha-arbutin 2%, and balance water. Tests show that the whitening essence milk containing the ionic liquid humanized III type collagen has the most obvious effect of improving skin melanin according to the mass percentage, and the skin has no adverse reaction after being used; the whitening essence milk containing the ionic liquid humanized type III collagen is proved to have the efficacy of permeation and absorption of alpha-arbutin and reduce the safety risk of products although the alpha-arbutin is less than 3%.
In addition, the invention also provides a preparation method of the whitening essence milk containing the ionic liquid humanized III type collagen, which comprises the following steps:
step S10: stirring and mixing C20-22 alcohol phosphate, cetostearyl alcohol, caprylic/capric triglyceride, isononyl isononanoate, hydrogenated polyisobutene, vitamin E acetate, tween-60, polydimethylsiloxane and dimethiconol at a first temperature to obtain a first mixture;
step S20: adding glycerol, betaine, glycerol glucoside, sodium polyacryloyldimethyl taurate, sphingomonas fermentation product extract and diglycerol into water, and stirring and dissolving at a first temperature to obtain a second mixture;
step S30: adding the first mixture into the second mixture, homogenizing, then stirring and cooling to a second temperature, adding the ionic liquid humanized type III collagen and the alpha-arbutin, and continuing stirring;
step S40: and finally, adding essence, stirring and cooling to a third temperature to prepare the whitening essence milk containing the ionic liquid humanized III type collagen.
The preparation method of the whitening essence milk containing the ionic liquid humanized type III collagen is simple in process, and the ionic liquid humanized type III collagen and the alpha-arbutin are added when the temperature of the mixed solution is reduced to the second temperature, so that the active ingredients of the ionic liquid humanized type III collagen and the alpha-arbutin can be effectively stored, and the ionic liquid humanized type III collagen and the alpha-arbutin have a synergistic effect in the whitening essence milk, namely the ionic liquid humanized type III collagen is used for improving the permeability of the alpha-arbutin in the skin, so that the bioavailability of the alpha-arbutin is improved, the content of the ionic liquid humanized type III collagen in the whitening essence milk is reduced under the condition of the same whitening effect, and the safety of a whitening product is ensured.
In some embodiments, the first temperature is 80-85 ℃; the second temperature is less than 50 ℃; the third temperature is 35-40 ℃. Under the temperature range, the biological activity and functional bonds of each component can be retained to the maximum extent, so that the whitening essence milk achieves the maximum whitening effect.
In some embodiments, the method of preparing the ionic liquid humanized type III collagen comprises the steps of:
step S100: providing an L-carnitine citrate ionic liquid, wherein the structural formula of the L-carnitine citrate ionic liquid is as follows:
step S200: and mixing the L-carnitine citrate ionic liquid and the humanized III type collagen solution, stirring, and then sequentially homogenizing, filtering, dialyzing and concentrating to obtain the ionic liquid humanized III type collagen.
According to the embodiment of the invention, the humanized type III collagen is delivered by adopting the L-carnitine citric acid ionic liquid, so that the delivery efficiency of the humanized type III collagen is improved while the excellent performance of the L-carnitine citric acid ionic liquid is maintained, and the bioavailability of the alpha-arbutin is improved.
In some embodiments, the mass ratio of the l-carnitine citrate ionic liquid to the humanized type III collagen is 10; too much or too little addition of the L-carnitine citrate ionic liquid can cause unstable properties of the obtained ionic liquid humanized type III collagen, and the effect of improving the delivery efficiency cannot be achieved.
In some embodiments, the step of mixing the l-carnitine citrate ionic liquid and the humanized type III collagen solution specifically comprises: adding humanized III type collagen solution into the L-carnitine citric acid ionic liquid within 10-60 minutes under the conditions of stirring speed of 50-300 rpm and water bath at-5-40 ℃; under the condition, the humanized III-type collagen solution is added into the L-carnitine citrate ionic liquid, so that the agglomeration of humanized III-type collagen can be effectively avoided, and the humanized III-type collagen is wrapped by the L-carnitine citrate ionic liquid.
In some embodiments, in step S30, the conditions of the homogenization process include: the pressure is 0-80MPa, the flow is 30-60L/h, and the homogenizing time is 3-5min. This step may allow the first mixture and the second mixture to be thoroughly mixed using a homogenization process.
The present invention will be described in further detail with reference to examples. It is also to be understood that the following examples are illustrative of the present invention and are not to be construed as limiting the scope of the invention, and that certain insubstantial modifications and adaptations of the invention by those skilled in the art may be made in light of the above teachings.
Specifically, table 1 is a formulation list of the whitening essence milk containing the ionic liquid humanized type III collagen in percentage by mass of comparative example 1 and examples 1 to 5.
The preparation method of the whitening essence milk containing the ionic liquid humanized III type collagen is utilized to respectively prepare the whitening essence milk corresponding to the comparative example 1 and the examples 1-5, and then the whitening essence milk is respectively subjected to a human body whitening efficacy test, wherein the test scheme is as follows:
1. the number of tested persons: 15 persons, age: 25-45 years old.
2. The test method comprises the following steps: the test sample is tried on the face of a subject, and the whitening and freckle removing effects of the test sample are comprehensively evaluated through instrument detection, and the test is implemented by referring to' method for testing whitening and whitening effects of cosmetics (second method) in a revision summary table of a method for testing related cosmetics by informing accessories of a method for testing related cosmetics in 2021 of the national drug administration.
3. The test types are as follows: random, self-back-and-forth control tests;
4. and (3) testing environment: RH at 20-22 deg.C and 40-60% humidity;
5. evaluation indexes are as follows: skin melanin MI value and color spot area (mm) 2 )；
Number of measurements and time points: a total of 2 measurements, day0 (Day 1) and Day28 (Day 28), respectively;
6. the test method comprises the following steps: instrumental testing
1) Thoroughly cleaning the face with a mild face cleaning product, and then standing in a constant temperature and humidity laboratory for 20min;
2) Shooting a face Visia image;
3) Measuring MI value of melanin of facial skin;
4) Dispensing a test sample and teaching a method of use/recovering a test sample;
7. the results of the melanin tests at D0 and D28 are shown in table 2, and the MI difference between D28 and D0 is shown in table 3; the results of the melanin tests at D0 and D28 are presented as a bar graph in FIG. 1, and the MI difference between D28 and D0 is presented as a bar graph in FIG. 2.
8. The test results of the color spot area ratios at D0 and D28 are shown in table 4, and the difference between the color spot area ratios of D28 and D0 is shown in table 5; the test results of the ratio of the area of color stains between D0 and D28 are shown in the form of a histogram in fig. 3, and the ratio of the area of color stains between D28 and D0 is shown in the form of a histogram in fig. 4.
The melanin values of comparative example 1 and examples 1-5 were significantly improved (P < 0.05) by human trials of the product of each example 15 persons for 28 days; the area occupancy ratio of the color spots of comparative example 1 and examples 1-5 was significantly improved (P < 0.001); the whitening essence milk samples prepared in the comparative example 1 and the examples 1 to 5 have the whitening and freckle removing effects; as can be seen from the improvement values, the melanin improvement is most significant in example 2 and example 4, and example 3 times, example 1 and comparative example 1 are worse than example 3, and example 5 is the case with the least improvement.
The result shows that when the content of the alpha-arbutin is 2%, the ionic liquid humanized III type collagen of 2-3% is contained in the whitening essence milk, so that the bioavailability of the alpha-arbutin can be improved, and the whitening effect of the whitening essence milk can be improved.
9. The 30 examples of human closed patch tests for safety used in examples 1-5 and comparative example 1 were verified as follows:
comparative example 1: 6 cases of grade 1 adverse skin reactions occur in 0.5h, 4 cases of grade 1 adverse skin reactions occur in 24h, and no adverse skin reactions occur in 48h, which indicates that the sample in the comparative example 1 has adverse skin contact reactions on human bodies;
example 1: 3 cases of grade 1 adverse skin reactions occur in 0.5h, 2 cases of grade 1 adverse skin reactions occur in 24h, and no adverse skin reactions occur in 48h, which indicates that the sample in example 1 has no adverse contact reactions on human skin;
example 2: 3 cases of grade 1 adverse skin reactions occur in 0.5h, no adverse skin reactions occur in 24h, and no adverse skin reactions occur in 48h, which indicates that the sample in example 2 has no adverse contact reactions on human skin;
example 3: 1 case of grade 1 adverse skin reaction occurs in 0.5h, no adverse skin reaction occurs in 24h, and no adverse skin reaction occurs in 48h, which indicates that the sample in example 3 has no adverse contact reaction to human skin;
example 4: 1 case of 1 grade 1 adverse skin reaction occurs in 0.5h, no adverse skin reaction occurs in 24h, and no adverse skin reaction occurs in 48h, which indicates that the sample in example 4 has no adverse contact reaction to human skin;
example 5: no adverse reaction on skin occurs in 0.5h, no adverse reaction on skin occurs in 24h, and no adverse reaction on skin occurs in 48h, which indicates that the sample in example 5 has no adverse reaction on human skin;
and (4) patch conclusion: through 30 human body enclosed patch tests, the comparative example has adverse reaction of skin contact on human body, and the examples 1-5 have no adverse reaction; the ionic liquid humanized type III collagen has the effect of promoting permeation and absorption of the alpha-arbutin, and the safety risk of the product is reduced.
In conclusion, the invention provides the whitening essence milk containing the ionic liquid humanized III type collagen and the preparation method thereof, the Ionic Liquid (ILs) humanized III type collagen in the whitening essence milk containing the ionic liquid humanized III type collagen has the characteristics of low volatility, high stability, high conductivity, permeability promotion and the like, and the characteristics conform to the pharmaceutical concept of 'green chemistry'. In drug delivery, ILs have the effects of increasing drug solubility, improving drug stability, promoting drug permeability and the like, so that the permeability of alpha-arbutin in skin can be improved by adding the ionic liquid humanized type III collagen into the whitening essence milk, the addition amount of the alpha-arbutin is reduced on the premise of the same whitening effect of the whitening essence milk, high bioavailability at a lower concentration is realized, and continuous administration and sustained release effects are endowed.
It is to be understood that the invention is not limited to the examples described above, but that modifications and variations may be effected thereto by those of ordinary skill in the art in light of the foregoing description, and that all such modifications and variations are intended to be within the scope of the invention as defined by the appended claims.
Claims (10)
1. The whitening essence milk containing the ionic liquid humanized type III collagen is characterized by comprising the following components in percentage by mass:
the ionic liquid humanized III type collagen is prepared by the reaction of humanized III type collagen and L-carnitine citrate ionic liquid.
2. The whitening essence milk containing ionic liquid humanized type III collagen according to claim 1, characterized in that the emulsifier is selected from at least one of C20-22 alcohol phosphate, tween-60, PEG-20 methylgluco-sesquistearate, polyglyceryl-6 laurate, cetearyl glucoside, polyglyceryl-10 isostearate, methylgluco-sesquistearate; the emulsion stabilizer is at least one selected from the group consisting of cetostearyl alcohol, 22 alcohol, PEG-100 stearate and glyceryl stearate; the emollient is selected from one of squalane, jojoba oil, coco-caprylate/caprate, macadamia nut oil, camellia oil, hydrogenated polyisobutene, dimethiconol, vitamin E acetate, dimethicone, isononyl isononanoate, caprylic/capric triglyceride, and C12-15 alcohol benzoate.
3. The whitening essence milk containing ionic liquid humanized type III collagen according to claim 2, characterized in that the humectant is selected from one of glycerin, glycerol glucoside, butylene glycol, propylene glycol, sorbitol, betaine, diglycerin, sodium hyaluronate; the thickening agent is selected from one of sphingomonas ferment product extract, sodium polyacryloyldimethyl taurate, ammonium acryloyldimethyl taurate/VP copolymer, polyacrylamide, polyacrylate cross-linked polymer-6, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the aromatizer is at least one of essence, essential oil and flower water.
4. The whitening essence milk containing ionic liquid humanized type III collagen according to claim 3, characterized in that it consists of 2.5% by mass of C20-22 alcohol phosphate, 0.6% by mass of cetostearyl alcohol, 4% by mass of caprylic/capric triglyceride, 4% by mass of isononyl isononanoate, 3% by mass of hydrogenated polyisobutene, 1% by mass of vitamin E acetate, 600.2% by mass of tween, 3% by mass of polydimethylsiloxane, 1% by mass of dimethiconol, 6% by mass of glycerin, 3% by mass of betaine, 1.5% by mass of glycerol glucoside, 0.5% by mass of sodium polyacryloyldimethyl taurate, 0.15% by mass of sphingomonas bacterium fermentation product extract, 2% by mass of diglycerol, 0.02% by mass of essence, 3% by mass of ionic liquid humanized type III collagen, 2% by mass of α -arbutin, and the balance of water.
5. The preparation method of the whitening essence milk containing the ionic liquid humanized type III collagen is characterized by comprising the following steps of:
stirring and mixing C20-22 alcohol phosphate, cetostearyl alcohol, caprylic/capric triglyceride, isononyl isononanoate, hydrogenated polyisobutene, vitamin E acetate, tween-60, polydimethylsiloxane and dimethiconol at a first temperature to obtain a first mixture;
adding glycerol, betaine, glycerol glucoside, sodium polyacryloyldimethyl taurate, sphingomonas fermentation product extract and diglycerol into water, and stirring and dissolving at a first temperature to obtain a second mixture;
adding the first mixture into the second mixture, homogenizing, then stirring and cooling to a second temperature, adding the ionic liquid humanized type III collagen and the alpha-arbutin, and continuing stirring;
and finally, adding essence, stirring and cooling to a third temperature to prepare the whitening essence milk containing the ionic liquid humanized type III collagen.
6. The method for preparing the whitening essence milk containing the ionic liquid humanized type III collagen according to claim 5, wherein the first temperature is 80-85 ℃; the second temperature is less than 50 ℃; the third temperature is 35-40 ℃.
7. The method of claim 5, wherein the method of preparing the ionic liquid humanized type III collagen comprises the steps of:
providing an L-carnitine citrate ionic liquid, wherein the structural formula of the L-carnitine citrate ionic liquid is as follows:
and mixing the L-carnitine citrate ionic liquid and the humanized III type collagen solution, stirring, and then sequentially homogenizing, filtering, dialyzing and concentrating to obtain the ionic liquid humanized III type collagen.
8. The preparation method of the whitening essence milk containing the ionic liquid humanized type III collagen according to claim 7, characterized in that the mass ratio of the L-carnitine citrate ionic liquid to the humanized type III collagen is 10.
9. The method for preparing the whitening essence milk containing ionic liquid humanized type III collagen according to claim 7, characterized in that the step of mixing the L-carnitine citrate ionic liquid and the humanized type III collagen solution specifically comprises:
adding the humanized III type collagen solution into the L-carnitine citrate ionic liquid within 10-60 minutes under the conditions of 50-300 rpm stirring speed and water bath at-5-40 ℃.
10. The method for preparing the whitening essence milk containing the ionic liquid humanized type III collagen according to claim 5, characterized in that the homogenization treatment conditions comprise: the pressure is 0-80MPa, the flow is 30-60L/h, and the homogenizing time is 3-5min.
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